LOC105648432 cDNA ORF clone, Jatropha curcas

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105648432
Entrez Gene ID	105648432
Full Name	proline--tRNA ligase, chloroplastic/mitochondrial
Gene Type	protein-coding
Organism	Jatropha curcas

mRNA and Protein(s)

mRNA	Protein	Name
XM_012234813.2	XP_012090203.1	proline--tRNA ligase, chloroplastic/mitochondrial

Pathways from BioSystems
Gene Ontology

The following LOC105648432 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105648432 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OJa64798	XM_012234813.2 Latest version!	Jatropha curcas proline--tRNA ligase, chloroplastic/mitochondrial (LOC105648432), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105648432 ( XM_012234813.2 ) cDNA ORF clone, Jatropha curcas -> XP_012090203.1 Jatropha curcas proline--tRNA ligase, chloroplastic/mitochondrial (LOC105648432), mRNA.

Price & Availability↑

CloneID	OJa64798
Clone ID Related Accession (Same CDS sequence)	XM_012234813.2
Accession Version	XM_012234813.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-05
Organism	Jatropha curcas
Product	proline--tRNA ligase, chloroplastic/mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012125302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 6, 2017 this sequence version replaced XM_012234813.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Jatropha curcas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTGT CTCTTAGACT ACCTTCTCTC ACTTCCCTCT TTTTCCATAC TCCATCAATT 
CGCCGGTCGT CGGCGTTTTG TAGGCGGCGC CACTATTGCC GTTCGCCGCC TCTAACATCG 
TCCTTCTCTG CTCAGAGTGC GGCTTCGAGC CCTCCGGAGA CCGAGGAACG AGTTGATAAC 
CTGAAGAATC GAGTCAACCA GGACCGAGTC ATTACTCCCC GATCTCAAGA CTTCAATGCT 
TGGTATCTCG ATGTTATTGC CAACGCCGAG CTTGCTGATT ATGGTCCTGT TCGTGGTACT 
ATGGTAATTC GTCCTTATGG ATATGCTATC TGGGAGGCAA TCCAGGATTA TTTAAATGTT 
AAGTTCAAGG AGACTGGTCA CAGCAACATG TACTTTCCTC AGTTTATACC TTACTCGTTT 
ATAGAGAAAG AAGCTAGTCA TGTTGAGGGC TTTAGTCCTG AATTGGCTTT GGTAACTATT 
GGTGGAGGAA AGGAGCTTGA AGAAAAACTT GTGGTTCGAC CTACTAGTGA AACTATAGTG 
AATCACATGT TTACTCAGTG GATTCATAGT TACCGCGATC TTCCTCTCAT GATCAACCAG 
TGGGCAAATG TCACAAGATG GGAGATGCGC ACAAAACCAT TTGTGAGGAC TCTTGAGTTT 
CTCTGGCAAG AGGGTCATAC AGCTCATGCT ACTCCTGAGG AAGCGGAAAA AGAGGCTTTA 
CAGATGATTG AAGTCTATAC AAAATTTGCT TATGAGCAAG CTGCAATACC TGTTGTTGCA 
GGTCGAAAGT CAAGGTTGGA AACCTTTGCT GGCGCTGTTA GGACATATAC AATAGAGGCT 
ATGATGGGTG ATCGTAAGGC ATTACAGGCT GGAACCAGCC ACAACCTTGG CCAAAATTTT 
TCTCGGGCTT TTGGAACTCA GTTCACTGAC GAAAGTGGAC AAAGGCAGCA TGTCTGGCAG 
ACATCATGGG CCATCAGTAC CCGCTTTGTT GGTGGTATTA TCATGACACA TGGAGATGAT 
GCAGGCTTAA TGCTTCCCCC CAAAGTAGCA CCAATACAGG TTGTGATTGT GCCAATTTGG 
AAGAAGGATA ATGAGAAAGC TGGAGTTCTC AGTGCAGCAT CATCTGTGAA AGAAGTTCTG 
CAATCTGCTG GTCTCAAAGT TAAACTTGAT GATTCAGATC AGAGAACCCC AGGATGGAAG 
TTCAATTTCT GGGAGATGAA GGGAGTACCT TTGAGGATTG AAATTGGTCC TCGAGATGTA 
TCAAGTGGAA GCGTGGTCAT ATCTAGGAGA GATATTCCTG GTAAGCAAGG AAAAGTTTTT 
GGAATATCTA TGGAGCCTTC AATTTTAGAA GGTTATGTCA AGGACAAGTT GGATGAGATC 
CAGTCATCCC TTTTGGAAAG AGCAACATCA TATCGGGACA GTAATATTGT GGATGTTAGC 
TCGTATGATG AACTCAAAGA AGCAATATCC CTTGGCAAAT GGGCAAGGGG TCCTTGGTCA 
GCCAGTGATG CTGATGAATT AAAGGTGAAA GAAGAAACAG GAGCAACAAT TCGATGTTTT 
CCCTTTGAAC AGCCTCAAGG GACTAAAACA TGTTTGATGA CTGGCGAGCC AGCAGAAGAA 
GTTGCGATTT TTGCTAAATC TTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012090203.1
CDS	119..1765
Translation	MVVSLRLPSLTSLFFHTPSIRRSSAFCRRRHYCRSPPLTSSFSAQSAASSPPETEERVDNLKNRVNQDRVITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQDYLNVKFKETGHSNMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHATPEEAEKEALQMIEVYTKFAYEQAAIPVVAGRKSRLETFAGAVRTYTIEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDESGQRQHVWQTSWAISTRFVGGIIMTHGDDAGLMLPPKVAPIQVVIVPIWKKDNEKAGVLSAASSVKEVLQSAGLKVKLDDSDQRTPGWKFNFWEMKGVPLRIEIGPRDVSSGSVVISRRDIPGKQGKVFGISMEPSILEGYVKDKLDEIQSSLLERATSYRDSNIVDVSSYDELKEAISLGKWARGPWSASDADELKVKEETGATIRCFPFEQPQGTKTCLMTGEPAEEVAIFAKSY

Target ORF information:

RefSeq Version	XM_012234813.2
Organism	Jatropha curcas
Definition	Jatropha curcas proline--tRNA ligase, chloroplastic/mitochondrial (LOC105648432), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012234813.2

ORF Insert Sequence:

ATGGTGGTGT CTCTTAGACT ACCTTCTCTC ACTTCCCTCT TTTTCCATAC TCCATCAATT 
CGCCGGTCGT CGGCGTTTTG TAGGCGGCGC CACTATTGCC GTTCGCCGCC TCTAACATCG 
TCCTTCTCTG CTCAGAGTGC GGCTTCGAGC CCTCCGGAGA CCGAGGAACG AGTTGATAAC 
CTGAAGAATC GAGTCAACCA GGACCGAGTC ATTACTCCCC GATCTCAAGA CTTCAATGCT 
TGGTATCTCG ATGTTATTGC CAACGCCGAG CTTGCTGATT ATGGTCCTGT TCGTGGTACT 
ATGGTAATTC GTCCTTATGG ATATGCTATC TGGGAGGCAA TCCAGGATTA TTTAAATGTT 
AAGTTCAAGG AGACTGGTCA CAGCAACATG TACTTTCCTC AGTTTATACC TTACTCGTTT 
ATAGAGAAAG AAGCTAGTCA TGTTGAGGGC TTTAGTCCTG AATTGGCTTT GGTAACTATT 
GGTGGAGGAA AGGAGCTTGA AGAAAAACTT GTGGTTCGAC CTACTAGTGA AACTATAGTG 
AATCACATGT TTACTCAGTG GATTCATAGT TACCGCGATC TTCCTCTCAT GATCAACCAG 
TGGGCAAATG TCACAAGATG GGAGATGCGC ACAAAACCAT TTGTGAGGAC TCTTGAGTTT 
CTCTGGCAAG AGGGTCATAC AGCTCATGCT ACTCCTGAGG AAGCGGAAAA AGAGGCTTTA 
CAGATGATTG AAGTCTATAC AAAATTTGCT TATGAGCAAG CTGCAATACC TGTTGTTGCA 
GGTCGAAAGT CAAGGTTGGA AACCTTTGCT GGCGCTGTTA GGACATATAC AATAGAGGCT 
ATGATGGGTG ATCGTAAGGC ATTACAGGCT GGAACCAGCC ACAACCTTGG CCAAAATTTT 
TCTCGGGCTT TTGGAACTCA GTTCACTGAC GAAAGTGGAC AAAGGCAGCA TGTCTGGCAG 
ACATCATGGG CCATCAGTAC CCGCTTTGTT GGTGGTATTA TCATGACACA TGGAGATGAT 
GCAGGCTTAA TGCTTCCCCC CAAAGTAGCA CCAATACAGG TTGTGATTGT GCCAATTTGG 
AAGAAGGATA ATGAGAAAGC TGGAGTTCTC AGTGCAGCAT CATCTGTGAA AGAAGTTCTG 
CAATCTGCTG GTCTCAAAGT TAAACTTGAT GATTCAGATC AGAGAACCCC AGGATGGAAG 
TTCAATTTCT GGGAGATGAA GGGAGTACCT TTGAGGATTG AAATTGGTCC TCGAGATGTA 
TCAAGTGGAA GCGTGGTCAT ATCTAGGAGA GATATTCCTG GTAAGCAAGG AAAAGTTTTT 
GGAATATCTA TGGAGCCTTC AATTTTAGAA GGTTATGTCA AGGACAAGTT GGATGAGATC 
CAGTCATCCC TTTTGGAAAG AGCAACATCA TATCGGGACA GTAATATTGT GGATGTTAGC 
TCGTATGATG AACTCAAAGA AGCAATATCC CTTGGCAAAT GGGCAAGGGG TCCTTGGTCA 
GCCAGTGATG CTGATGAATT AAAGGTGAAA GAAGAAACAG GAGCAACAAT TCGATGTTTT 
CCCTTTGAAC AGCCTCAAGG GACTAAAACA TGTTTGATGA CTGGCGAGCC AGCAGAAGAA 
GTTGCGATTT TTGCTAAATC TTATTAA