PRODH cDNA ORF clone, Jatropha curcas

Gene
Clones
gRNAs

Summary

Gene Symbol	PRODH
Entrez Gene ID	105646802
Full Name	proline dehydrogenase (oxidase) 1
Gene Type	protein-coding
Organism	Jatropha curcas

mRNA and Protein(s)

mRNA	Protein	Name
NM_001308804.1	NP_001295733.1	proline dehydrogenase 1, mitochondrial

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Molecular cloning and expression analysis of the gene encoding proline dehydrogenase from Jatropha curcas L.
Wang H, Ao P, Yang S, Zou Z, Wang S, Gong M
Applied biochemistry and biotechnology175(5)2413-26(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PRODH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRODH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OJa39719	NM_001308804.1 Latest version!	Jatropha curcas proline dehydrogenase (oxidase) 1 (PRODH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRODH ( NM_001308804.1 ) cDNA ORF clone, Jatropha curcas -> NP_001295733.1 Jatropha curcas proline dehydrogenase (oxidase) 1 (PRODH), mRNA.

Price & Availability↑

CloneID	OJa39719
Clone ID Related Accession (Same CDS sequence)	NM_001308804.1
Accession Version	NM_001308804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-29
Organism	Jatropha curcas
Product	proline dehydrogenase 1, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from KF879446.1. On May 15, 2015 this sequence version replaced XM_012232741.1. ##Evidence-Data-START## Transcript exon combination :: KF879446.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00188808, SAMN01894327 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCCATCA AATCCCTTTC TCCAAAACTT CTCAAAAACC TCCGTTTTTT CATTAGACCT 
CTCAACTCCT CCACCTCTTC CTCCTCCATC TCCGCCATCT CCTCACTTAA CTTGGCTCAA 
AAACCCGAAC CCGCCCTTGC CCCTGCCTCC GCCACGGTAA CCAAAGCACC ATCAATTCTA 
GATTTAAACA ATCATCAAAG CCTTTTCTCC TCTATACCCA CTAAAAAGCT TTTACATGCT 
TCTCTTATCC TTCAAATGGC GGCTTTAGAG CCTATAGTTG ATTTGGGCAT GTGGGTTCTG 
AATTCGAGGT TGATGGAGAT GGAAATCGTA CGTAATGTTA TATTGGGAGT TGTACGACGC 
AGTTTTTATG AACATTTCTG TGGTGGTGAA AATAGTTTCG AGGCTGTAAA GTGTGTAAGG 
AGATTGAATG AGTTTGGATT AAGAGCTAAG ATGGATTTTG CTGTGGAGTT TACTGTTGAT 
AATGATGCTT GTGATCGGAA TTTTAATGGG TTTCTTCAAG CTATTGAAGC AGCTAAATCT 
CTTCCTTCAT CTTCTGTGAG TTCTGTGGTT GTAAAGATAA CAGCAATTTG CCCTCTGAAC 
CTACTTGAGA GGATTAGTGA TCAATTGAGG TGGCAACAGA AAGACCCATC TTTAAATCTT 
CCATGGAAAG ATGACACTTT TCCAATTTTC TCTAATTCAA GCCCTTTATA TCACACACTA 
AAAAAACCAG AACCATTAAC CATTGAAGAA GAAGAAGGTC TTCTCTTAGC CCAACAAAGA 
CTACTCAAAC TGTGCGAAAA ATGTGTTGAA GCCAATTTAC CATTAGTGAT TGATGCAGAG 
CACACAGCAG TTCAACCTGC AATTGACTAC TTGACGTATT TATCAGCAAT TAAGTATAAT 
AAAGATGACA ATCCAATTGT CTATGGAACG ATTCAAGCTT ATTTGAAAGA TGCAAAAGAG 
AGATTGTTAT TAGCAACAAA AGCAGCTGAT AAAACGGGAG TTCCTATGGG ATTCAAGTTA 
GTAAGAGGTG CTTATATGTC AAGTGAAAGA AAATTAGCAT CTTCTTTAGG ATATGAATCT 
CCAATTCATA ATTCCATTCA AGAAACTCAT GATTGCTTCA ATGATTGTGC TTCTATCTTG 
CTTGAAACGA TCTCTAATGG CTCGCATGGA GTCATTTTGG CAACACATAA TGTTGAATCA 
GGGAAATTGG CTGCAAAAAG AGCAATAGAG TTGGGAATTG GGACTGGAAA TTATCAGAAG 
CTTGAGTTTG CACAGCTATA TGGAATGGCA GAAGTGCTTT CTTTGAGTCT AAAAAATGCA 
GGTTTTCAAG TAAGCAAGTA TATGCCTTAT GGACCTATAG ATACAGTTAT ACCATATCTT 
CTAAGGAGAG CTGAAGAAAA CAGGGGACTC TTATCTGCTT CAAGCCTTGA CAGAGACCTC 
TTTAGGAAGG AATTGACCAG AAGACTAAAA GCTGCAATCT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295733.1
CDS	1..1485
Translation	MAIKSLSPKLLKNLRFFIRPLNSSTSSSSISAISSLNLAQKPEPALAPASATVTKAPSILDLNNHQSLFSSIPTKKLLHASLILQMAALEPIVDLGMWVLNSRLMEMEIVRNVILGVVRRSFYEHFCGGENSFEAVKCVRRLNEFGLRAKMDFAVEFTVDNDACDRNFNGFLQAIEAAKSLPSSSVSSVVVKITAICPLNLLERISDQLRWQQKDPSLNLPWKDDTFPIFSNSSPLYHTLKKPEPLTIEEEEGLLLAQQRLLKLCEKCVEANLPLVIDAEHTAVQPAIDYLTYLSAIKYNKDDNPIVYGTIQAYLKDAKERLLLATKAADKTGVPMGFKLVRGAYMSSERKLASSLGYESPIHNSIQETHDCFNDCASILLETISNGSHGVILATHNVESGKLAAKRAIELGIGTGNYQKLEFAQLYGMAEVLSLSLKNAGFQVSKYMPYGPIDTVIPYLLRRAEENRGLLSASSLDRDLFRKELTRRLKAAIF

Target ORF information:

RefSeq Version	NM_001308804.1
Organism	Jatropha curcas
Definition	Jatropha curcas proline dehydrogenase (oxidase) 1 (PRODH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308804.1

ORF Insert Sequence:

ATGGCCATCA AATCCCTTTC TCCAAAACTT CTCAAAAACC TCCGTTTTTT CATTAGACCT 
CTCAACTCCT CCACCTCTTC CTCCTCCATC TCCGCCATCT CCTCACTTAA CTTGGCTCAA 
AAACCCGAAC CCGCCCTTGC CCCTGCCTCC GCCACGGTAA CCAAAGCACC ATCAATTCTA 
GATTTAAACA ATCATCAAAG CCTTTTCTCC TCTATACCCA CTAAAAAGCT TTTACATGCT 
TCTCTTATCC TTCAAATGGC GGCTTTAGAG CCTATAGTTG ATTTGGGCAT GTGGGTTCTG 
AATTCGAGGT TGATGGAGAT GGAAATCGTA CGTAATGTTA TATTGGGAGT TGTACGACGC 
AGTTTTTATG AACATTTCTG TGGTGGTGAA AATAGTTTCG AGGCTGTAAA GTGTGTAAGG 
AGATTGAATG AGTTTGGATT AAGAGCTAAG ATGGATTTTG CTGTGGAGTT TACTGTTGAT 
AATGATGCTT GTGATCGGAA TTTTAATGGG TTTCTTCAAG CTATTGAAGC AGCTAAATCT 
CTTCCTTCAT CTTCTGTGAG TTCTGTGGTT GTAAAGATAA CAGCAATTTG CCCTCTGAAC 
CTACTTGAGA GGATTAGTGA TCAATTGAGG TGGCAACAGA AAGACCCATC TTTAAATCTT 
CCATGGAAAG ATGACACTTT TCCAATTTTC TCTAATTCAA GCCCTTTATA TCACACACTA 
AAAAAACCAG AACCATTAAC CATTGAAGAA GAAGAAGGTC TTCTCTTAGC CCAACAAAGA 
CTACTCAAAC TGTGCGAAAA ATGTGTTGAA GCCAATTTAC CATTAGTGAT TGATGCAGAG 
CACACAGCAG TTCAACCTGC AATTGACTAC TTGACGTATT TATCAGCAAT TAAGTATAAT 
AAAGATGACA ATCCAATTGT CTATGGAACG ATTCAAGCTT ATTTGAAAGA TGCAAAAGAG 
AGATTGTTAT TAGCAACAAA AGCAGCTGAT AAAACGGGAG TTCCTATGGG ATTCAAGTTA 
GTAAGAGGTG CTTATATGTC AAGTGAAAGA AAATTAGCAT CTTCTTTAGG ATATGAATCT 
CCAATTCATA ATTCCATTCA AGAAACTCAT GATTGCTTCA ATGATTGTGC TTCTATCTTG 
CTTGAAACGA TCTCTAATGG CTCGCATGGA GTCATTTTGG CAACACATAA TGTTGAATCA 
GGGAAATTGG CTGCAAAAAG AGCAATAGAG TTGGGAATTG GGACTGGAAA TTATCAGAAG 
CTTGAGTTTG CACAGCTATA TGGAATGGCA GAAGTGCTTT CTTTGAGTCT AAAAAATGCA 
GGTTTTCAAG TAAGCAAGTA TATGCCTTAT GGACCTATAG ATACAGTTAT ACCATATCTT 
CTAAGGAGAG CTGAAGAAAA CAGGGGACTC TTATCTGCTT CAAGCCTTGA CAGAGACCTC 
TTTAGGAAGG AATTGACCAG AAGACTAAAA GCTGCAATCT TTTAG