LOC108277567 cDNA ORF clone, Ictalurus punctatus(channel catfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108277567
Entrez Gene ID	108277567
Full Name	E3 ubiquitin/ISG15 ligase TRIM25-like
Gene Type	protein-coding
Organism	Ictalurus punctatus(channel catfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017490387.1	XP_017345876.1	E3 ubiquitin/ISG15 ligase TRIM25-like

Pathways from BioSystems
Gene Ontology

The following LOC108277567 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108277567 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIc12447	XM_017490387.1 Latest version!	Ictalurus punctatus E3 ubiquitin/ISG15 ligase TRIM25-like (LOC108277567), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108277567 ( XM_017490387.1 ) cDNA ORF clone, Ictalurus punctatus(channel catfish) -> XP_017345876.1 Ictalurus punctatus E3 ubiquitin/ISG15 ligase TRIM25-like (LOC108277567), mRNA.

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CloneID	OIc12447
Clone ID Related Accession (Same CDS sequence)	XM_017490387.1
Accession Version	XM_017490387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-05
Organism	Ictalurus punctatus(channel catfish)
Product	E3 ubiquitin/ISG15 ligase TRIM25-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030432.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictalurus punctatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCAAAGG CTAGTACTTT AAAATATGAT GAAGCGTTGA CCTGTCCAGC ATGTCTGGAA 
TTACTGAAGG ATCCAATGAC TACTCCCTGT AACCACAATT TCTGTAATGT GTGTATTAAT 
CGTTGCTGGT GTGAGGATAA GCTGGGCATC TACAGCTGTC CCCAGTGCAG AGAGACTTTC 
AATTCAAGGC CTGATCTACA CAGGAACAAC ATGCTGGCTG AAATGGTGAA GAAACTGAAG 
AAGACTGAAC TCCAAGCTGC TTCTCCTGCT CACTGTTACG CTGGACCTGG AGATGTGGAG 
TGTGATTCCT GCATTGGGAG AAAACACAAA GCCATCAAGT CCTGTCTGGT GTGTCTGGCC 
TCCTTTTGTG AAGATCATCT TAAACCTCAC TATCAGTCCT CTGCCTTTAA GAAGCACAAG 
TTAGTTGAAG CCTGTGCAGA GCTCCAAGAG AAGATCTGCT CTAAGCATGA CAAACCGATC 
GAGATCTACT GTCGTACCGA CCAAAGCTTC ATCTGTTACT TATGTATGAT GGATGAACAC 
AAAACCCATG ATACTGTTGC AGCTGAAGCT GAAAGAACTG AAAAACAGAA TGAGCTAAAG 
GAGGAGCAGC TGAAATCCCA GCAGAAGATA CAGGAGAAGC AGAAGAAGGT GCAGGAGCTG 
AAAAAGGCTC TGGATACTAT TAAGAGGCAT TTACAAGCAG CCGTGGACGA CAGTGAGAGG 
CCCTTTACTG AGATTAACTT ACACTCTGAA GTGAAGGAGC TGATCAGAGA TCAGGAGAAA 
GCTGAACTGA GTCGAGCTGA ACGACTCCTG AAGCAACTGG AGCAAGAGAT TGCTGCTCTT 
AAGAGGAGAG TCACTGAGCT GGAGCAGCTT TCACACACAC ACGATCACAT CCACTTCCTC 
AAGAGTTTCC CGTCTCTCTG TGTTTCTCCT GGATCTGAGG ACTCACCCAG CATCACTGTC 
AATCAACATC TCTCATTTGA TGGAGTGTGG AAATTTCCCT CATATCTGGA AAAACGAGTC 
GAGGAAATCT GTGAGGAGGA ATTCAACAAA ATCCGTCCAC AAGCTACATC AGTTTGGATA 
CATCTATCCC CGGAACCAAA GAGAAAAGAA GATTTTCTGA AGTATTTCTT TTATCTAACC 
CTGGATCCCA ACACAGTACA TCATCAACTC ATACTCTCTG AGAGGAACAG AGCGGTGACA 
TACTGTGAGA GAAAACAGGG ATACTCTGAT CATACAGAGA GATTTGGCTC CTGGTCTCAG 
GTGTTGTGTA AGGAGAGAGT GTGTGGACGC TGTTACTGGG AGGTGGAGTG GAGCACTGCT 
GAGTTTGTGG ATATATCAGT CTCATATATT GGGATTAGGA GGAAAGGACT GGGTTTCAAG 
AGTGGGTTTG GGTACAACAG TCAGTCCTGG AGTCTGGAGT TGTCTCGTTT TTCTGTCTTT 
TTTCGGCACA ACGACACTGA GACTAAGATC AGAGGTCCAG TATCCTCCAG AATAGGAGTG 
TATGTAGATC ACAGTGCAGG AACTCTGTCC TTCTACAGCA TCTCTGACAC GATGAGGCTC 
CTCCACAGAG TCCACACCAC ATTCACTCAG CCTTTATACG CTGGGTTTAC TCTGTGCAAT 
GCAGGATCAA CTGTGAGGTT ATGTAATCTA AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017345876.1
CDS	52..1707
Translation	MAKASTLKYDEALTCPACLELLKDPMTTPCNHNFCNVCINRCWCEDKLGIYSCPQCRETFNSRPDLHRNNMLAEMVKKLKKTELQAASPAHCYAGPGDVECDSCIGRKHKAIKSCLVCLASFCEDHLKPHYQSSAFKKHKLVEACAELQEKICSKHDKPIEIYCRTDQSFICYLCMMDEHKTHDTVAAEAERTEKQNELKEEQLKSQQKIQEKQKKVQELKKALDTIKRHLQAAVDDSERPFTEINLHSEVKELIRDQEKAELSRAERLLKQLEQEIAALKRRVTELEQLSHTHDHIHFLKSFPSLCVSPGSEDSPSITVNQHLSFDGVWKFPSYLEKRVEEICEEEFNKIRPQATSVWIHLSPEPKRKEDFLKYFFYLTLDPNTVHHQLILSERNRAVTYCERKQGYSDHTERFGSWSQVLCKERVCGRCYWEVEWSTAEFVDISVSYIGIRRKGLGFKSGFGYNSQSWSLELSRFSVFFRHNDTETKIRGPVSSRIGVYVDHSAGTLSFYSISDTMRLLHRVHTTFTQPLYAGFTLCNAGSTVRLCNLK

Target ORF information:

RefSeq Version	XM_017490387.1
Organism	Ictalurus punctatus(channel catfish)
Definition	Ictalurus punctatus E3 ubiquitin/ISG15 ligase TRIM25-like (LOC108277567), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017490387.1

ORF Insert Sequence:

ATGGCAAAGG CTAGTACTTT AAAATATGAT GAAGCGTTGA CCTGTCCAGC ATGTCTGGAA 
TTACTGAAGG ATCCAATGAC TACTCCCTGT AACCACAATT TCTGTAATGT GTGTATTAAT 
CGTTGCTGGT GTGAGGATAA GCTGGGCATC TACAGCTGTC CCCAGTGCAG AGAGACTTTC 
AATTCAAGGC CTGATCTACA CAGGAACAAC ATGCTGGCTG AAATGGTGAA GAAACTGAAG 
AAGACTGAAC TCCAAGCTGC TTCTCCTGCT CACTGTTACG CTGGACCTGG AGATGTGGAG 
TGTGATTCCT GCATTGGGAG AAAACACAAA GCCATCAAGT CCTGTCTGGT GTGTCTGGCC 
TCCTTTTGTG AAGATCATCT TAAACCTCAC TATCAGTCCT CTGCCTTTAA GAAGCACAAG 
TTAGTTGAAG CCTGTGCAGA GCTCCAAGAG AAGATCTGCT CTAAGCATGA CAAACCGATC 
GAGATCTACT GTCGTACCGA CCAAAGCTTC ATCTGTTACT TATGTATGAT GGATGAACAC 
AAAACCCATG ATACTGTTGC AGCTGAAGCT GAAAGAACTG AAAAACAGAA TGAGCTAAAG 
GAGGAGCAGC TGAAATCCCA GCAGAAGATA CAGGAGAAGC AGAAGAAGGT GCAGGAGCTG 
AAAAAGGCTC TGGATACTAT TAAGAGGCAT TTACAAGCAG CCGTGGACGA CAGTGAGAGG 
CCCTTTACTG AGATTAACTT ACACTCTGAA GTGAAGGAGC TGATCAGAGA TCAGGAGAAA 
GCTGAACTGA GTCGAGCTGA ACGACTCCTG AAGCAACTGG AGCAAGAGAT TGCTGCTCTT 
AAGAGGAGAG TCACTGAGCT GGAGCAGCTT TCACACACAC ACGATCACAT CCACTTCCTC 
AAGAGTTTCC CGTCTCTCTG TGTTTCTCCT GGATCTGAGG ACTCACCCAG CATCACTGTC 
AATCAACATC TCTCATTTGA TGGAGTGTGG AAATTTCCCT CATATCTGGA AAAACGAGTC 
GAGGAAATCT GTGAGGAGGA ATTCAACAAA ATCCGTCCAC AAGCTACATC AGTTTGGATA 
CATCTATCCC CGGAACCAAA GAGAAAAGAA GATTTTCTGA AGTATTTCTT TTATCTAACC 
CTGGATCCCA ACACAGTACA TCATCAACTC ATACTCTCTG AGAGGAACAG AGCGGTGACA 
TACTGTGAGA GAAAACAGGG ATACTCTGAT CATACAGAGA GATTTGGCTC CTGGTCTCAG 
GTGTTGTGTA AGGAGAGAGT GTGTGGACGC TGTTACTGGG AGGTGGAGTG GAGCACTGCT 
GAGTTTGTGG ATATATCAGT CTCATATATT GGGATTAGGA GGAAAGGACT GGGTTTCAAG 
AGTGGGTTTG GGTACAACAG TCAGTCCTGG AGTCTGGAGT TGTCTCGTTT TTCTGTCTTT 
TTTCGGCACA ACGACACTGA GACTAAGATC AGAGGTCCAG TATCCTCCAG AATAGGAGTG 
TATGTAGATC ACAGTGCAGG AACTCTGTCC TTCTACAGCA TCTCTGACAC GATGAGGCTC 
CTCCACAGAG TCCACACCAC ATTCACTCAG CCTTTATACG CTGGGTTTAC TCTGTGCAAT 
GCAGGATCAA CTGTGAGGTT ATGTAATCTA AAATGA