ch25h cDNA ORF clone, Ictalurus punctatus(channel catfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ch25h
Entrez Gene ID	100528885
Full Name	cholesterol 25-hydroxylase
Gene Type	protein-coding
Organism	Ictalurus punctatus(channel catfish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001201155.1	NP_001188084.1	cholesterol 25-hydroxylase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).
Chen F, Lee Y, Jiang Y, Wang S, Peatman E, Abernathy J, Liu H, Liu S, Kucuktas H, Ke C, Liu Z
PloS one5(7)e11546(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ch25h gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ch25h cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIc38795	NM_001201155.1 Latest version!	Ictalurus punctatus cholesterol 25-hydroxylase (ch25h), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ch25h ( NM_001201155.1 ) cDNA ORF clone, Ictalurus punctatus(channel catfish) -> NP_001188084.1 Ictalurus punctatus cholesterol 25-hydroxylase (ch25h), mRNA.

Price & Availability↑

CloneID	OIc38795
Clone ID Related Accession (Same CDS sequence)	NM_001201155.1
Accession Version	NM_001201155.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-04
Organism	Ictalurus punctatus(channel catfish)
Product	cholesterol 25-hydroxylase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GU589076.1. ##Evidence-Data-START## Transcript exon combination :: GU589076.1 [ECO:0000332] ##Evidence-Data-END##

ATGTCTCAGC TCACACAGGA CCAGAGCAAC ATATCTCAGC TCACACAGGC ACAGAGCAAC 
ATGTCTCAGC TCCAGGGAAT CTGGAACTGT ATCCTACTGT ATGAGACCTG GTTACGGCCT 
CCCCTTTTCC CTGTACTGTT TTCACTGGCA GTCTACTTAA GCTTCTGTTT ACCTTTTGTG 
CTTCTGGACC TCCTGTCCTC CAGAGTGGAC CTTGTGAGGA GATACAAAAT CCAGCAGAAA 
CCAGTGACCT TGAGGATGAT GTGGAACTGC CTGGCTCTCT CCCTCTACAA TCATGTGGTG 
TACATCTTCC CGCTGAGCGT GCTGCACTGG TACTGGAGAC CAGTCATCTA CCCAGCAGAG 
GCTCCCAGGG TCCTTGAAGT CATCGGGCAC TTGGTGAGCT GTTTGTTGCT CTTCGATTTC 
CAGTACTTCA TCTGGCATGT GCTGCATCAC AAGGTGCCAT GGCTTTACCG CACATTCCAC 
AAGGTGCACC ATAAGCACAC GTCCACCTTC GTCCTCACTA CAGAGTACTC GGGTGCTTGG 
GAGACGCTCT CACTCGGCCT CTTTGCAGCG ATGAACCCCA TGCTGTTGGA CTGCCATCCG 
TTCACTGAGA TGCTTTTTCA TATCCTGAAC ATGTGGCTGT CAGTCGAGGA CCACTGTAGG 
TATGACCTGC CCTGGGCCAC ACACAGACTC GTTCCTTTTG GACTGTATGG AGGCGCGCCA 
CATCATGACC TTCATCACCA GAAGTTCAAG GCCAACTACG CTCCATACTT TACTCACTGG 
GACAAGCTGT TCGGGACACT TCACTCAGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188084.1
CDS	19..831
Translation	MSQLTQDQSNISQLTQAQSNMSQLQGIWNCILLYETWLRPPLFPVLFSLAVYLSFCLPFVLLDLLSSRVDLVRRYKIQQKPVTLRMMWNCLALSLYNHVVYIFPLSVLHWYWRPVIYPAEAPRVLEVIGHLVSCLLLFDFQYFIWHVLHHKVPWLYRTFHKVHHKHTSTFVLTTEYSGAWETLSLGLFAAMNPMLLDCHPFTEMLFHILNMWLSVEDHCRYDLPWATHRLVPFGLYGGAPHHDLHHQKFKANYAPYFTHWDKLFGTLHSE

Target ORF information:

RefSeq Version	NM_001201155.1
Organism	Ictalurus punctatus(channel catfish)
Definition	Ictalurus punctatus cholesterol 25-hydroxylase (ch25h), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201155.1

ORF Insert Sequence:

ATGTCTCAGC TCACACAGGA CCAGAGCAAC ATATCTCAGC TCACACAGGC ACAGAGCAAC 
ATGTCTCAGC TCCAGGGAAT CTGGAACTGT ATCCTACTGT ATGAGACCTG GTTACGGCCT 
CCCCTTTTCC CTGTACTGTT TTCACTGGCA GTCTACTTAA GCTTCTGTTT ACCTTTTGTG 
CTTCTGGACC TCCTGTCCTC CAGAGTGGAC CTTGTGAGGA GATACAAAAT CCAGCAGAAA 
CCAGTGACCT TGAGGATGAT GTGGAACTGC CTGGCTCTCT CCCTCTACAA TCATGTGGTG 
TACATCTTCC CGCTGAGCGT GCTGCACTGG TACTGGAGAC CAGTCATCTA CCCAGCAGAG 
GCTCCCAGGG TCCTTGAAGT CATCGGGCAC TTGGTGAGCT GTTTGTTGCT CTTCGATTTC 
CAGTACTTCA TCTGGCATGT GCTGCATCAC AAGGTGCCAT GGCTTTACCG CACATTCCAC 
AAGGTGCACC ATAAGCACAC GTCCACCTTC GTCCTCACTA CAGAGTACTC GGGTGCTTGG 
GAGACGCTCT CACTCGGCCT CTTTGCAGCG ATGAACCCCA TGCTGTTGGA CTGCCATCCG 
TTCACTGAGA TGCTTTTTCA TATCCTGAAC ATGTGGCTGT CAGTCGAGGA CCACTGTAGG 
TATGACCTGC CCTGGGCCAC ACACAGACTC GTTCCTTTTG GACTGTATGG AGGCGCGCCA 
CATCATGACC TTCATCACCA GAAGTTCAAG GCCAACTACG CTCCATACTT TACTCACTGG 
GACAAGCTGT TCGGGACACT TCACTCAGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).
PloS one5(7)e11546(2010 Jul)
Chen F,Lee Y,Jiang Y,Wang S,Peatman E,Abernathy J,Liu H,Liu S,Kucuktas H,Ke C,Liu Z