crim2 cDNA ORF clone, Hydra vulgaris

Gene
Clones
gRNAs

Summary

Gene Symbol	crim2
Entrez Gene ID	100189550
Full Name	cysteine rich BMP regulator 2
Gene Type	protein-coding
Organism	Hydra vulgaris

mRNA and Protein(s)

mRNA	Protein	Name
NM_001280890.1	NP_001267819.1	cysteine rich BMP regulator 2 precursor

Pathways from BioSystems
Gene Ontology

The following crim2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crim2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHc13121	NM_001280890.1 Latest version!	Hydra vulgaris cysteine rich BMP regulator 2 (crim2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crim2 ( NM_001280890.1 ) cDNA ORF clone, Hydra vulgaris -> NP_001267819.1 Hydra vulgaris cysteine rich BMP regulator 2 (crim2), mRNA.

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CloneID	OHc13121
Clone ID Related Accession (Same CDS sequence)	NM_001280890.1
Accession Version	NM_001280890.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-04
Organism	Hydra vulgaris
Product	cysteine rich BMP regulator 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY225467.1. On Jul 24, 2013 this sequence version replaced XM_002161355.2. ##Evidence-Data-START## Transcript exon combination :: AY225467.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1711201, SAMEA1711202 [ECO:0000350] ##Evidence-Data-END##

ATGAAGAGCA TGAAACTGTT ACTTTTGCAA TTATTATTCT TTGGAGTTTA CAGCAAACCC 
GCTACAAAAG TAATTCTAGA TGCATCTGAT AAAACCTGTC CTCCCTGCAA TGAAGAATGC 
CCAACTTTAG ACAAATGTGA TTTTGGAACA GTAAAAGAAA AATGTGGTTG CTGTAAGGTT 
TGCATTCGAA ATGAAGGAGA TATTTGTGGT GGAATTAATG AAATTTATGG AGTTTGCAAA 
GAAGGCTTAG TTTGTAAAGT TCGACATCCG TTTTCTCTTA AAAATTTAAA TCTAGAGATG 
TCAGATAAAA TAGGGCGCTG TGAGACAGAG CTTTGCAGCA GTACAACATG CGGAAGGCAT 
CAGTGGTGTA AGGTTGATAA TGAGTTACCA ACATGTTTTT GCAGAAATGA CTGTGAATCG 
GATGAAGAAA AGTTGTGTGA TAATGATGGT AATAAGTATA AAAACATATG TGAACTGCGC 
AGTAAAGAAT GTAAACTCCA ACAAAAAATT CCGCACTTTT CATGCAAAGA TTGCCAACGT 
ACTGCAGAAA GTAAATTAGA AACAATTGAG TCTGGTGTTG TTCTAAACAC ATCCAGGATG 
TGTGAAAAAG AAGAATGTGT AAATGGGGTA TTTAAAGTAT CATTAGACAA TACTTGTTTA 
AAGGCAGAAG GTTTTACTTG TTCGGAAGGT TCAAACATTC ATCAGGATAT CACCTGCCCA 
GTAGGATATT ACTGTAATAT AACAAAATTA GAGCTTAACC CTGAGTATGG ATATCCAAAT 
CTTGGACAAT GTTCTGCAAT TGATAGCTCT GAAGTTTGTA AACTTGCACC AGATACTGGT 
GCTTGCTACG CTTATTTTCC AAGATGGCAT TTTGACATTT CTACTGGTAC TTGCAAAGAG 
TTTATTTATG GTGGTTGCCA GGGTAACAAA AATAATTTCA AGTCAAAAGA TGAATGTCTT 
AGATTGTGTG GAGATAGAGA TAAGCCTTTA GCAGCAATGC CAATGAATGC GTTTCCTTTT 
GTGGGTATGC CAATGTTTCC TTTTGCTGGC AAAGTCCAGG AAAGACCAAT GAGTGATAAA 
TCAATGACCA TAAGTGGATT AGTTATTTTA CCACAAACCA TCCAAATTCC TCCAAAATCG 
TGTTTAGTTG TTCATTTCCA AGATGTCACT TTAGCAGATG TTGCATCCCG GATTATAGCT 
ACTCAAATTG TACATTTTTC TGAGGTCACC ACTAAAGATG TAATTGATTA TAAGATTAAT 
AGTAAGATGC CCTTACCAGA AGATATCGGT AGAATATACT CTGTTAGAGC ATTTCTAAAC 
ATTGGTTGGT GCAGCAGAAC AGTAGATGAT GGTAGATCTA TTCAAAAGAA AGATTATCTT 
TCAGATGAGA GAACCACAGT TATGTTAAGT AATGACAAAG ATGAATATAT AAAAGATATT 
ACTATAAAAT GTTATGACTG TGATAAAACA GATTTGCTTA CTGATATACA AATGGGATTT 
CCAATGGAAG AACCTGAATT TGAACTAGAA AAGAAAGAAG AATCTGATTT TAGTGGTGAT 
GATGATACGG ATTCCAAATT TCCTTATGAT GGACCTCAAA TGAGCAGCAC TGTGATAGAA 
ATAAGAGGTT CAGTTAAATT CTCTAATATC ACTGAAAGTG TATTAAAAAA AGATTCTTGC 
CTTTGGATCT CTTTGAGTGA TGTCACATTA CAAGACGCAA AATCTATAAC TCTTTCTTCA 
ACATTTATGG ATATGTCGTT CTACAAAGTA GGTAAAAGTA TTCAATATGT TCTTCAGTCA 
ATGAAACCGA TGAATGAAGA ATTGAGTCGT ACTTATGTGG TTCAAGCTGT CTTAAATAAC 
GGATGGTGTT ATAAAAAAGG TTCAGATAAA TGGCTCAAAA AAGGGGATTT TTTAAATACA 
GTAACCCACA CTATTAATCT AAATAAGGAA TCAAACAGCT ATAATTTAGA TGTTAACGTA 
ATATGCTATG AATGTGAAAC CAAAGAAGAT TTATCATCGG CCTCAAAATC TCTAAATAAT 
GGTAATGAAA TGATGTGGGT AAATGGTACA TTATTCTTTC CTGATGTACC TAAAGTCAAT 
GCATCGTCTT GCTTGATAGT TTCATTCAGA GATGTTTCTA TTGCTGATTA TAAGTCAAAA 
ACTCTCGCCA TGCTTGTGTT AAGCGTATCA CAATTTAAAG ACAAAGAATA TAACTATGTT 
CTCCAGATAA AAAAGCCATC CGATTTATCT GGACGTTTTA GTGTACATGC AGTACTAAAT 
GTAAACTGGT GCTCGTCAGA TTCATCTCAA AAATGGATTG AAAAAGGAGA CTACTTGACA 
GTTACTTCTT ACCAAGTTGA TCTTAAAGAA AATACAAACA CTTATAGCCA AGATGTGCAC 
CTAATTTGTT ACAGCTGTAT ATCAAGTTCT CAGAAAGAAG ATAGCAAAAA GTTTTGCCTG 
GATAAAAAAA ATAACATAAC TCGCAAACAC GATGAAACAT GGCTAGCTGA TCCATGTACT 
ACATGTGTGT GTGATGATGG ATTTTCAGCC TGTGCAATTA AAAGTTGTGT GTCTAACTGC 
CCTACACCAA TTCCAAAACC AGGAGAATGT TGTTCTCAAT GCTCAAGTAC ATGTTTATAT 
GAAAATAAAT TTTACAATGA AGGAGATGAA TGGTCCCCAG ATTCATGTAC TAAATGCAAT 
TGCATTAATG GGGAGAAATT ATGCTCAGTT GTTGATTGTC TACCAGATAG CATGTTGCCG 
TGCAAAAATC CTGTGTTGAT AGAAGGTAAT TGTTGCAGAT CATGTCCTAT ACTGATGTCA 
TCACCAGTAA AACAAGAATG TTTCTATGAA CAAACAAACA AAACTTATCA AGATGGTGAT 
GAGTGGACTT CGCCAGATTT TTGCGCTTCA TGTGTTTGTG ACAAAGGTAA TACCATGTGT 
GCAACACCAA TGTGTGCTTT ACCTCCGTGC TCAGAAGATA AGATAATTAA TATTCCAGGC 
AGATGTTGCC CTATTTGTCC TGAGACAAAT GTTTGTAAAG ACCTTAACAC CAATCGAGCA 
TATACTGAGG GAGAAATATG GCAAAATTCA GATTGTAATG TTTGTAGATG TACTTCAAAT 
GACACAGTTT GTGAAAAACC ATTAGCTGAT TATGAAAGTT GTGAAAACAA GGTTAAGTTA 
ACAAAAAATT GTCCTGCATT CTGCTTTATT GAAACGGACA GCAAGCCTAA ATGTGAAGAT 
AGTAGTAATT ATTACTCAGT CGGACAAGAA ATAGAAAGAG ATTGCAATAA ATGTATATGT 
GTTGAGCAAG GCAAATGGGA ATGTACTAAA CGTAGTTGTC CTATTATGAT AGAGGGCTTA 
CCATCTGGAG CAACAGATGA AGCTGAAGTA GCAGATGATG GTCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001267819.1
CDS	112..3519
Translation	MKSMKLLLLQLLFFGVYSKPATKVILDASDKTCPPCNEECPTLDKCDFGTVKEKCGCCKVCIRNEGDICGGINEIYGVCKEGLVCKVRHPFSLKNLNLEMSDKIGRCETELCSSTTCGRHQWCKVDNELPTCFCRNDCESDEEKLCDNDGNKYKNICELRSKECKLQQKIPHFSCKDCQRTAESKLETIESGVVLNTSRMCEKEECVNGVFKVSLDNTCLKAEGFTCSEGSNIHQDITCPVGYYCNITKLELNPEYGYPNLGQCSAIDSSEVCKLAPDTGACYAYFPRWHFDISTGTCKEFIYGGCQGNKNNFKSKDECLRLCGDRDKPLAAMPMNAFPFVGMPMFPFAGKVQERPMSDKSMTISGLVILPQTIQIPPKSCLVVHFQDVTLADVASRIIATQIVHFSEVTTKDVIDYKINSKMPLPEDIGRIYSVRAFLNIGWCSRTVDDGRSIQKKDYLSDERTTVMLSNDKDEYIKDITIKCYDCDKTDLLTDIQMGFPMEEPEFELEKKEESDFSGDDDTDSKFPYDGPQMSSTVIEIRGSVKFSNITESVLKKDSCLWISLSDVTLQDAKSITLSSTFMDMSFYKVGKSIQYVLQSMKPMNEELSRTYVVQAVLNNGWCYKKGSDKWLKKGDFLNTVTHTINLNKESNSYNLDVNVICYECETKEDLSSASKSLNNGNEMMWVNGTLFFPDVPKVNASSCLIVSFRDVSIADYKSKTLAMLVLSVSQFKDKEYNYVLQIKKPSDLSGRFSVHAVLNVNWCSSDSSQKWIEKGDYLTVTSYQVDLKENTNTYSQDVHLICYSCISSSQKEDSKKFCLDKKNNITRKHDETWLADPCTTCVCDDGFSACAIKSCVSNCPTPIPKPGECCSQCSSTCLYENKFYNEGDEWSPDSCTKCNCINGEKLCSVVDCLPDSMLPCKNPVLIEGNCCRSCPILMSSPVKQECFYEQTNKTYQDGDEWTSPDFCASCVCDKGNTMCATPMCALPPCSEDKIINIPGRCCPICPETNVCKDLNTNRAYTEGEIWQNSDCNVCRCTSNDTVCEKPLADYESCENKVKLTKNCPAFCFIETDSKPKCEDSSNYYSVGQEIERDCNKCICVEQGKWECTKRSCPIMIEGLPSGATDEAEVADDGP

Target ORF information:

RefSeq Version	NM_001280890.1
Organism	Hydra vulgaris
Definition	Hydra vulgaris cysteine rich BMP regulator 2 (crim2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001280890.1

ORF Insert Sequence:

ATGAAGAGCA TGAAACTGTT ACTTTTGCAA TTATTATTCT TTGGAGTTTA CAGCAAACCC 
GCTACAAAAG TAATTCTAGA TGCATCTGAT AAAACCTGTC CTCCCTGCAA TGAAGAATGC 
CCAACTTTAG ACAAATGTGA TTTTGGAACA GTAAAAGAAA AATGTGGTTG CTGTAAGGTT 
TGCATTCGAA ATGAAGGAGA TATTTGTGGT GGAATTAATG AAATTTATGG AGTTTGCAAA 
GAAGGCTTAG TTTGTAAAGT TCGACATCCG TTTTCTCTTA AAAATTTAAA TCTAGAGATG 
TCAGATAAAA TAGGGCGCTG TGAGACAGAG CTTTGCAGCA GTACAACATG CGGAAGGCAT 
CAGTGGTGTA AGGTTGATAA TGAGTTACCA ACATGTTTTT GCAGAAATGA CTGTGAATCG 
GATGAAGAAA AGTTGTGTGA TAATGATGGT AATAAGTATA AAAACATATG TGAACTGCGC 
AGTAAAGAAT GTAAACTCCA ACAAAAAATT CCGCACTTTT CATGCAAAGA TTGCCAACGT 
ACTGCAGAAA GTAAATTAGA AACAATTGAG TCTGGTGTTG TTCTAAACAC ATCCAGGATG 
TGTGAAAAAG AAGAATGTGT AAATGGGGTA TTTAAAGTAT CATTAGACAA TACTTGTTTA 
AAGGCAGAAG GTTTTACTTG TTCGGAAGGT TCAAACATTC ATCAGGATAT CACCTGCCCA 
GTAGGATATT ACTGTAATAT AACAAAATTA GAGCTTAACC CTGAGTATGG ATATCCAAAT 
CTTGGACAAT GTTCTGCAAT TGATAGCTCT GAAGTTTGTA AACTTGCACC AGATACTGGT 
GCTTGCTACG CTTATTTTCC AAGATGGCAT TTTGACATTT CTACTGGTAC TTGCAAAGAG 
TTTATTTATG GTGGTTGCCA GGGTAACAAA AATAATTTCA AGTCAAAAGA TGAATGTCTT 
AGATTGTGTG GAGATAGAGA TAAGCCTTTA GCAGCAATGC CAATGAATGC GTTTCCTTTT 
GTGGGTATGC CAATGTTTCC TTTTGCTGGC AAAGTCCAGG AAAGACCAAT GAGTGATAAA 
TCAATGACCA TAAGTGGATT AGTTATTTTA CCACAAACCA TCCAAATTCC TCCAAAATCG 
TGTTTAGTTG TTCATTTCCA AGATGTCACT TTAGCAGATG TTGCATCCCG GATTATAGCT 
ACTCAAATTG TACATTTTTC TGAGGTCACC ACTAAAGATG TAATTGATTA TAAGATTAAT 
AGTAAGATGC CCTTACCAGA AGATATCGGT AGAATATACT CTGTTAGAGC ATTTCTAAAC 
ATTGGTTGGT GCAGCAGAAC AGTAGATGAT GGTAGATCTA TTCAAAAGAA AGATTATCTT 
TCAGATGAGA GAACCACAGT TATGTTAAGT AATGACAAAG ATGAATATAT AAAAGATATT 
ACTATAAAAT GTTATGACTG TGATAAAACA GATTTGCTTA CTGATATACA AATGGGATTT 
CCAATGGAAG AACCTGAATT TGAACTAGAA AAGAAAGAAG AATCTGATTT TAGTGGTGAT 
GATGATACGG ATTCCAAATT TCCTTATGAT GGACCTCAAA TGAGCAGCAC TGTGATAGAA 
ATAAGAGGTT CAGTTAAATT CTCTAATATC ACTGAAAGTG TATTAAAAAA AGATTCTTGC 
CTTTGGATCT CTTTGAGTGA TGTCACATTA CAAGACGCAA AATCTATAAC TCTTTCTTCA 
ACATTTATGG ATATGTCGTT CTACAAAGTA GGTAAAAGTA TTCAATATGT TCTTCAGTCA 
ATGAAACCGA TGAATGAAGA ATTGAGTCGT ACTTATGTGG TTCAAGCTGT CTTAAATAAC 
GGATGGTGTT ATAAAAAAGG TTCAGATAAA TGGCTCAAAA AAGGGGATTT TTTAAATACA 
GTAACCCACA CTATTAATCT AAATAAGGAA TCAAACAGCT ATAATTTAGA TGTTAACGTA 
ATATGCTATG AATGTGAAAC CAAAGAAGAT TTATCATCGG CCTCAAAATC TCTAAATAAT 
GGTAATGAAA TGATGTGGGT AAATGGTACA TTATTCTTTC CTGATGTACC TAAAGTCAAT 
GCATCGTCTT GCTTGATAGT TTCATTCAGA GATGTTTCTA TTGCTGATTA TAAGTCAAAA 
ACTCTCGCCA TGCTTGTGTT AAGCGTATCA CAATTTAAAG ACAAAGAATA TAACTATGTT 
CTCCAGATAA AAAAGCCATC CGATTTATCT GGACGTTTTA GTGTACATGC AGTACTAAAT 
GTAAACTGGT GCTCGTCAGA TTCATCTCAA AAATGGATTG AAAAAGGAGA CTACTTGACA 
GTTACTTCTT ACCAAGTTGA TCTTAAAGAA AATACAAACA CTTATAGCCA AGATGTGCAC 
CTAATTTGTT ACAGCTGTAT ATCAAGTTCT CAGAAAGAAG ATAGCAAAAA GTTTTGCCTG 
GATAAAAAAA ATAACATAAC TCGCAAACAC GATGAAACAT GGCTAGCTGA TCCATGTACT 
ACATGTGTGT GTGATGATGG ATTTTCAGCC TGTGCAATTA AAAGTTGTGT GTCTAACTGC 
CCTACACCAA TTCCAAAACC AGGAGAATGT TGTTCTCAAT GCTCAAGTAC ATGTTTATAT 
GAAAATAAAT TTTACAATGA AGGAGATGAA TGGTCCCCAG ATTCATGTAC TAAATGCAAT 
TGCATTAATG GGGAGAAATT ATGCTCAGTT GTTGATTGTC TACCAGATAG CATGTTGCCG 
TGCAAAAATC CTGTGTTGAT AGAAGGTAAT TGTTGCAGAT CATGTCCTAT ACTGATGTCA 
TCACCAGTAA AACAAGAATG TTTCTATGAA CAAACAAACA AAACTTATCA AGATGGTGAT 
GAGTGGACTT CGCCAGATTT TTGCGCTTCA TGTGTTTGTG ACAAAGGTAA TACCATGTGT 
GCAACACCAA TGTGTGCTTT ACCTCCGTGC TCAGAAGATA AGATAATTAA TATTCCAGGC 
AGATGTTGCC CTATTTGTCC TGAGACAAAT GTTTGTAAAG ACCTTAACAC CAATCGAGCA 
TATACTGAGG GAGAAATATG GCAAAATTCA GATTGTAATG TTTGTAGATG TACTTCAAAT 
GACACAGTTT GTGAAAAACC ATTAGCTGAT TATGAAAGTT GTGAAAACAA GGTTAAGTTA 
ACAAAAAATT GTCCTGCATT CTGCTTTATT GAAACGGACA GCAAGCCTAA ATGTGAAGAT 
AGTAGTAATT ATTACTCAGT CGGACAAGAA ATAGAAAGAG ATTGCAATAA ATGTATATGT 
GTTGAGCAAG GCAAATGGGA ATGTACTAAA CGTAGTTGTC CTATTATGAT AGAGGGCTTA 
CCATCTGGAG CAACAGATGA AGCTGAAGTA GCAGATGATG GTCCATGA