KNTC1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017020254.1	XP_016875743.1	kinetochore-associated protein 1 isoform X3
XM_017020254.2	XP_016875743.1	kinetochore-associated protein 1 isoform X3
XM_017020257.1	XP_016875746.1	kinetochore-associated protein 1 isoform X8
NM_014708.6	NP_055523.1	kinetochore-associated protein 1
NM_014708.5	NP_055523.1	kinetochore-associated protein 1
XM_011539029.3	XP_011537331.1	kinetochore-associated protein 1 isoform X5
XM_017020256.2	XP_016875745.1	kinetochore-associated protein 1 isoform X6
XM_011539030.1	XP_011537332.1	kinetochore-associated protein 1 isoform X7
XM_017020255.2	XP_016875744.1	kinetochore-associated protein 1 isoform X4
XM_011539029.2	XP_011537331.1	kinetochore-associated protein 1 isoform X5
NM_014708.4	NP_055523.1	kinetochore-associated protein 1
XM_006719706.2	XP_006719769.1	kinetochore-associated protein 1 isoform X1
XM_011539027.3	XP_011537329.1	kinetochore-associated protein 1 isoform X2
XM_024449285.1	XP_024305053.1	kinetochore-associated protein 1 isoform X9
XM_011539027.2	XP_011537329.1	kinetochore-associated protein 1 isoform X2
XM_017020255.1	XP_016875744.1	kinetochore-associated protein 1 isoform X4
XM_017020256.1	XP_016875745.1	kinetochore-associated protein 1 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu103807	XM_024449285.1 Latest version!	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu84343	XM_017020257.1 Latest version!	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu84342	XM_017020256.2 Latest version!	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu63243	XM_011539029.3 Latest version!	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu63244	XM_011539030.1 Latest version!	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu84340	XM_017020254.2 Latest version!	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu84341	XM_017020255.2 Latest version!	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu10474	NM_014708.6 Latest version!	Homo sapiens kinetochore associated 1 (KNTC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu10474	XM_006719706.2 Latest version!	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu63241	XM_011539027.3 Latest version!	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu84340	XM_017020254.1	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu10474	NM_014708.5	Homo sapiens kinetochore associated 1 (KNTC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu63243	XM_011539029.2	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu10474	NM_014708.4	Homo sapiens kinetochore associated 1 (KNTC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu63241	XM_011539027.2	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu84341	XM_017020255.1	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu84342	XM_017020256.1	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

KNTC1 ( XM_024449285.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024305053.1 Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu103807
Clone ID Related Accession (Same CDS sequence)	XM_024449285.1
Accession Version	XM_024449285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	kinetochore-associated protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAACCA 
GTTTTCAGGC AACATTTTCT CACCAAGAAA GACCTCATTA AAGCTCTTGT GGAGAATATA 
GATATGGACA CAAGCCTCAT TTTGGAATAT TGCAGGCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024305053.1
CDS	146..4507
Translation	MWNDIELLTNDDTGSGYLSVGSRKEHGTALYQVDLLVKISSEKASLNPKIQACSLSDGFIIVADQSVILLDSICRSLQLHLVFDTEVDVVGLCQEGKFLLVGERSGNLHLIHVTSKQTLLTNAFVQKANDENRRTYQNLVIEKDGSNEGTYYMLLLTYSGFFCITNLQLLKIQQAIENVDFSTAKKLQGQIKSSFISTENYHTLGCLSLVAGDLASEVPVIIGGTGNCAFSKWEPDSSKKGMTVKNLIDAEIIKGAKKFQLIDNLLFVLDTDNVLSLWDIYTLTPVWNWPSLHVEEFLLTTEADSPSSVTWQGITNLKLIALTASANKKMKNLMVYSLPTMEILYSLEVSSVSSLVQTGISTDTIYLLEGVCKNDPKLSEDSVSVLVLRCLTEALPENRLSRLLHKHRFAEAESFAIQFGLDVELVYKVKSNHILEKLALSSVDASEQTEWQQLVDDAKENLHKIQDDEFVVNYCLKAQWITYETTQEMLNYAKTRLLKKEDKTALIYSDGLKEVLRAHAKLTTFYGAFGPEKFSGSSWIEFLNNEDDLKDIFLQLKEGNLVCAQYLWLRHRANFESRFDVKMLESLLNSMSASVSLQKLCPWFKNDVIPFVRRTVPEGQIILAKWLEQAARNLELTDKANWPENGLQLAEIFFTAEKTDELGLASSWHWISLKDYQNTEEVCQLRTLVNNLRELITLHRKYNCKLALSDFEKENTTTIVFRMFDKVLAPELIPSILEKFIRVYMREHDLQEEELLLLYIEDLLNRCSSKSTSLFETAWEAKAMAVIACLSDTDLIFDAVLKIMYAAVVPWSAAVEQLVKQHLEMDHPKVKLLQESYKLMEMKKLLRGYGIREVNLLNKEIMRVVRYILKQDVPSSLEDALKVAQAFMLSDDEIYSLRIIDLIDREQGEDCLLLLKSLPPAEAEKTAERVIIWARLALQEEPDHSKEGKAWRMSVAKTSVDILKILCDIQKDNLQKKDECEEMLKLFKEVASLQENFEVFLSFEDYSNSSLVADLREQHIKAHEVAQAKHKPGSTPEPIAAEVRSPSMESKLHRQALALQMSKQELEAELTLRALKDGNIKTALKKCSDLFKYHCNADTGKLLFLTCQKLCQMLADNVPVTVPVGLNLPSMIHDLASQAATICSPDFLLDALELCKHTLMAVELSRQCQMDDCGILMKASFGTHKDPYEEWSYSDFFSEDGIVLESQMVLPVIYELISSLVPLAESKRYPLESTSLPYCSLNEGDGLVLPVINSISALLQNLQESSQWELALRFVVGSFGTCLQHSVSNFMNATLSEKLFGETTLVKSRHVVMELKEKAVIFIRENATTLLHKVFNCRLVDLDLALGYCTLLPQKDVFENLWKLIDKAWQNYDKILAISLVGSELASLYQEIEMGLKFRELSTDAQWGIRLGKLGISFQPVFRQHFLTKKDLIKALVENIDMDTSLILEYCRH

Target ORF information:

RefSeq Version	XM_024449285.1
Organism	Homo sapiens(human)
Definition	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024449285.1

ORF Insert Sequence:

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAACCA 
GTTTTCAGGC AACATTTTCT CACCAAGAAA GACCTCATTA AAGCTCTTGT GGAGAATATA 
GATATGGACA CAAGCCTCAT TTTGGAATAT TGCAGGCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNTC1 ( XM_017020257.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875746.1 Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu84343
Clone ID Related Accession (Same CDS sequence)	XM_017020257.1
Accession Version	XM_017020257.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	kinetochore-associated protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTCTGTT TGCAATAATC 
TTGCTACTGC TCACTCTTTG GGTCCACGCT GTTTTTATGA GCTGTGAGAC TCACATGAAG 
ATCTGCAGCT TCTTTCCTGA GTGGAGACCA CGAGCCCACC GAGAGGAAGG AACAACTCCA 
GACGCTCTGC CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875746.1
CDS	146..4540
Translation	MWNDIELLTNDDTGSGYLSVGSRKEHGTALYQVDLLVKISSEKASLNPKIQACSLSDGFIIVADQSVILLDSICRSLQLHLVFDTEVDVVGLCQEGKFLLVGERSGNLHLIHVTSKQTLLTNAFVQKANDENRRTYQNLVIEKDGSNEGTYYMLLLTYSGFFCITNLQLLKIQQAIENVDFSTAKKLQGQIKSSFISTENYHTLGCLSLVAGDLASEVPVIIGGTGNCAFSKWEPDSSKKGMTVKNLIDAEIIKGAKKFQLIDNLLFVLDTDNVLSLWDIYTLTPVWNWPSLHVEEFLLTTEADSPSSVTWQGITNLKLIALTASANKKMKNLMVYSLPTMEILYSLEVSSVSSLVQTGISTDTIYLLEGVCKNDPKLSEDSVSVLVLRCLTEALPENRLSRLLHKHRFAEAESFAIQFGLDVELVYKVKSNHILEKLALSSVDASEQTEWQQLVDDAKENLHKIQDDEFVVNYCLKAQWITYETTQEMLNYAKTRLLKKEDKTALIYSDGLKEVLRAHAKLTTFYGAFGPEKFSGSSWIEFLNNEDDLKDIFLQLKEGNLVCAQYLWLRHRANFESRFDVKMLESLLNSMSASVSLQKLCPWFKNDVIPFVRRTVPEGQIILAKWLEQAARNLELTDKANWPENGLQLAEIFFTAEKTDELGLASSWHWISLKDYQNTEEVCQLRTLVNNLRELITLHRKYNCKLALSDFEKENTTTIVFRMFDKVLAPELIPSILEKFIRVYMREHDLQEEELLLLYIEDLLNRCSSKSTSLFETAWEAKAMAVIACLSDTDLIFDAVLKIMYAAVVPWSAAVEQLVKQHLEMDHPKVKLLQESYKLMEMKKLLRGYGIREVNLLNKEIMRVVRYILKQDVPSSLEDALKVAQAFMLSDDEIYSLRIIDLIDREQGEDCLLLLKSLPPAEAEKTAERVIIWARLALQEEPDHSKEGKAWRMSVAKTSVDILKILCDIQKDNLQKKDECEEMLKLFKEVASLQENFEVFLSFEDYSNSSLVADLREQHIKAHEVAQAKHKPGSTPEPIAAEVRSPSMESKLHRQALALQMSKQELEAELTLRALKDGNIKTALKKCSDLFKYHCNADTGKLLFLTCQKLCQMLADNVPVTVPVGLNLPSMIHDLASQAATICSPDFLLDALELCKHTLMAVELSRQCQMDDCGILMKASFGTHKDPYEEWSYSDFFSEDGIVLESQMVLPVIYELISSLVPLAESKRYPLESTSLPYCSLNEGDGLVLPVINSISALLQNLQESSQWELALRFVVGSFGTCLQHSVSNFMNATLSEKLFGETTLVKSRHVVMELKEKAVIFIRENATTLLHKVFNCRLVDLDLALGYCTLLPQKDVFENLWKLIDKAWQNYDKILAISLVGSELASLYQEIEMGLKFRELSTDAQWGIRLGKLGLFAIILLLLTLWVHAVFMSCETHMKICSFFPEWRPRAHREEGTTPDALP

Target ORF information:

RefSeq Version	XM_017020257.1
Organism	Homo sapiens(human)
Definition	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020257.1

ORF Insert Sequence:

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTCTGTT TGCAATAATC 
TTGCTACTGC TCACTCTTTG GGTCCACGCT GTTTTTATGA GCTGTGAGAC TCACATGAAG 
ATCTGCAGCT TCTTTCCTGA GTGGAGACCA CGAGCCCACC GAGAGGAAGG AACAACTCCA 
GACGCTCTGC CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNTC1 ( XM_017020256.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875745.1 Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X6, mRNA.

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CloneID	OHu84342
Clone ID Related Accession (Same CDS sequence)	XM_017020256.2 , XM_017020256.1
Accession Version	XM_017020256.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	kinetochore-associated protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017020256.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAGA GCTTGCTCAA CTCAATGTCT GCATCAGTCT CTTTGCAAAA GCTGTGTCCA 
TGGTTTAAAA ATGATGTGAT TCCATTTGTA AGAAGGACTG TGCCTGAAGG ACAGATAATT 
CTTGCAAAAT GGTTGGAACA AGCAGCCAGG AACCTTGAAT TAACTGATAA GGCAAATTGG 
CCAGAAAATG GACTTCAATT GGCAGAGATA TTTTTTACAG CAGAAAAAAC AGACGAGTTG 
GGATTGGCAT CTTCCTGGCA TTGGATTTCC TTGAAAGATT ATCAGAACAC AGAGGAAGTA 
TGTCAGCTAA GGACTTTGGT AAATAACTTG CGAGAGTTGA TCACGTTGCA TAGGAAGTAC 
AACTGCAAAT TAGCCCTCTC TGATTTTGAG AAGGAAAATA CAACCACCAT AGTGTTCCGA 
ATGTTTGATA AAGTGCTGGC CCCAGAGCTT ATTCCCTCCA TCTTAGAGAA GTTTATAAGA 
GTTTACATGA GAGAACATGA CTTGCAAGAG GAGGAACTTC TCTTGCTGTA CATAGAGGAT 
TTACTGAATA GATGCAGCTC AAAGTCCACA TCACTCTTTG AAACAGCATG GGAAGCAAAG 
GCCATGGCAG TAATAGCGTG TTTATCTGAC ACGGACCTCA TATTTGATGC CGTGCTCAAG 
ATCATGTATG CGGCAGTGGT TCCTTGGAGT GCAGCTGTGG AGCAACTGGT GAAACAGCAC 
CTGGAAATGG ACCATCCCAA AGTCAAGTTA TTACAGGAAA GTTACAAACT AATGGAGATG 
AAAAAACTTT TACGAGGCTA TGGAATAAGA GAGGTAAATC TCTTAAACAA GGAAATAATG 
AGAGTGGTTA GATACATTCT CAAACAAGAT GTCCCATCTT CTTTAGAAGA TGCTTTAAAG 
GTAGCCCAAG CGTTTATGTT ATCTGATGAT GAGATCTACA GTCTAAGAAT TATTGACCTG 
ATTGATAGAG AACAGGGTGA AGACTGTCTC CTTCTGTTGA AGTCTTTGCC TCCTGCTGAA 
GCTGAGAAAA CTGCAGAAAG AGTCATCATA TGGGCACGAC TGGCATTACA AGAAGAGCCA 
GATCATTCTA AAGAGGGCAA GGCCTGGAGA ATGTCTGTAG CGAAGACATC CGTGGACATT 
CTTAAGATAC TATGTGACAT TCAGAAAGAC AATCTGCAGA AGAAGGACGA ATGTGAAGAA 
ATGTTGAAAC TATTTAAAGA GGTTGCTAGC TTACAGGAGA ACTTTGAGGT CTTTCTTTCA 
TTTGAAGATT ATAGCAATAG TTCCCTGGTA GCAGATCTCC GTGAGCAGCA CATTAAAGCT 
CACGAAGTTG CACAGGCGAA ACACAAACCT GGGAGCACCC CAGAGCCCAT AGCTGCTGAG 
GTGAGGAGCC CAAGCATGGA ATCAAAGCTG CACAGACAGG CACTGGCCCT GCAGATGTCC 
AAACAAGAGC TGGAGGCAGA GCTGACCTTG AGAGCCTTAA AAGATGGGAA CATCAAAACA 
GCACTGAAAA AATGCAGCGA CTTGTTTAAG TATCACTGCA ATGCTGACAC TGGGAAATTG 
CTATTTCTGA CATGTCAGAA GCTTTGTCAG ATGTTGGCTG ATAATGTCCC AGTGACAGTG 
CCTGTGGGAC TGAATCTTCC TTCCATGATA CATGATCTAG CAAGCCAAGC TGCCACCATT 
TGCAGTCCAG ATTTTTTACT AGATGCTTTA GAACTATGTA AACATACTTT AATGGCTGTA 
GAGCTTTCCA GACAATGCCA AATGGATGAC TGTGGAATCC TCATGAAAGC TTCTTTTGGG 
ACACATAAAG ATCCATATGA AGAGTGGTCT TACAGTGACT TCTTCAGTGA AGATGGAATT 
GTTCTTGAGT CACAGATGGT GCTTCCAGTG ATTTATGAAC TGATTTCATC TCTTGTGCCT 
CTAGCTGAAA GCAAGAGATA TCCCTTGGAG TCTACCAGTT TGCCATACTG CTCCCTTAAT 
GAAGGAGATG GCCTTGTTTT ACCTGTTATA AATTCCATCT CTGCCCTGCT TCAGAATCTT 
CAGGAATCTA GCCAGTGGGA GCTAGCCCTA AGATTTGTGG TTGGTTCATT TGGTACCTGT 
CTTCAGCACT CTGTGTCAAA CTTCATGAAT GCCACTTTGA GTGAAAAGTT ATTTGGAGAG 
ACTACATTAG TTAAATCAAG GCATGTTGTT ATGGAATTGA AAGAAAAAGC TGTTATATTT 
ATCAGGGAAA ATGCTACAAC ACTACTGCAC AAAGTATTTA ATTGTCGCTT GGTAGATCTT 
GACCTGGCGT TGGGTTACTG CACTCTCTTA CCTCAAAAAG ATGTGTTTGA AAATCTCTGG 
AAGCTCATAG ATAAAGCATG GCAGAATTAC GACAAAATCT TGGCAATATC TCTGGTGGGC 
TCTGAGCTGG CAAGTCTCTA TCAGGAAATA GAAATGGGGC TTAAGTTCCG TGAACTCAGT 
ACTGATGCCC AGTGGGGCAT TCGTCTTGGT AAACTTGGTA TTTCTTTTCA ACCAGTTTTC 
AGGCAACATT TTCTCACCAA GAAAGACCTC ATTAAAGCTC TTGTGGAGAA TATAGATATG 
GACACAAGCC TCATTTTGGA ATATTGCAGC ACATTTCAGT TGGACTGCGA TGCAGTTCTT 
CAGCTCTTCA TTGAAACGCT GCTCCACAAC ACAAATGCCG GCCAAGGCCA GGGAGATGCA 
AGCATGGACT CTGCAAAGCG GCGGCATCCC AAACTCCTGG CCAAAGCCCT TGAGATGGTT 
CCTTTACTGA CGAGCACAAA AGATTTGGTC ATCAGTCTTA GTGGAATACT ACATAAGTTG 
GATCCTTATG ACTATGAAAT GATTGAAGTT GTCTTGAAAG TTATAGAACG AGCTGATGAA 
AAGATAACCA ATATTAATAT TAATCAGGCA TTGAGTATTC TGAAACATTT GAAGTCATAC 
AGAAGAATTT CTCCTCCCGT GGATCTAGAA TATCAGTATA TGTTGGAACA TGTCATAACT 
TTGCCATCAG CTGCCCAAAC TAGACTGCCT TTTCACCTGA TATTCTTTGG CACAGCACAG 
AACTTCTGGA AAATTCTCTC TACAGAACTC AGTGAAGAAT CTTTCCCAAC ATTGCTCTTA 
ATTTCGAAAT TAATGAAGTT CTCTCTGGAC ACTCTGTACG TGTCTACAGC AAAACACGTT 
TTCGAAAAAA AACTGAAGCC AAAGCTCCTG AAGTTAACAC AAGCTAAATC CTCAACACTG 
ATTAACAAGG AAATAACTAA GATCACGCAG ACCATCGAAT CCTGCTTACT CTCTATAGTC 
AACCCAGAGT GGGCTGTAGC TATTGCCATC AGCCTTGCCC AGGATATCCC TGAAGGTTCC 
TTCAAGATAT CTGCTTTGAA ATTCTGCCTT TATTTAGCTG AGAGATGGCT ACAGAATATC 
CCATCGCAGG ACGAAAAACG TGAAAAAGCC GAGGCTTTGT TGAAGAAGCT TCATATCCAG 
TACCGGCGAT CGGGCACAGA AGCTGTGCTC ATAGCCCACA AGCTGAACAC TGAGGAATAT 
TTAAGAGTGA TCGGAAAGCC AGCACATCTT ATTGTCAGTC TCTACGAACA TCCTAGCATC 
AATCAAAGAA TTCAGAATTC ATCTGGCACA GATTATCCTG ATATTCATGC AGCAGCTAAA 
GAAATAGCCG AAGTCAATGA AATTAATTTG GAAAAAGTCT GGGACATGTT GTTGGAAAAA 
TGGCTATGCC CTTCAACAAA ACCTGGTGAA AAACCATCAG AATTATTTGA ACTTCAAGAA 
GATGAAGCCC TACGAAGAGT GCAGTATCTC CTCCTGTCTC GTCCAATTGA TTATAGTTCA 
AGAATGCTGT TTGTATTTGC AACATCAACT ACAACCACAT TAGGTATGCA TCAGTTAACT 
TTTGCCCATA GAACTCGAGC TCTTCAGTGT CTCTTCTATT TGGCTGACAA GGAAACTATA 
GAATCTCTCT TTAAAAAACC CATTGAAGAA GTGAAATCTT ATTTGAGATG TATAACTTTT 
CTGGCATCAT TTGAGACTTT GAATATCCCC ATCACATATG AATTATTTTG CAGCAGTCCT 
AAAGAAGGAA TGATTAAGGG TCTGTGGAAA AACCACAGCC ACGAGTCCAT GGCAGTAAGA 
TTGGTGACTG AGCTGTGTTT AGAATACAAA ATCTATGACC TGCAGCTTTG GAATGGACTC 
TTGCAAAAGC TTCTGGGCTT CAATATGATT CCTTATCTAA GGAAAGTTTT AAAAGCCATC 
TCCAGTATCC ATTCTTTATG GCAGGTTCCC TACTTCAGCA AAGCGTGGCA GCGTGTGATA 
CAGATACCAC TGCTTTCAGC CTCTTGTCCT TTAAGTCCTG ATCAGCTGTC AGATTGTTCT 
GAGAGTCTCA TCGCTGTCCT CGAATGTCCA GTCTCAGGTG ATCTTGACCT GATCGGAGTC 
GCCAGGCAGT ATATCCAGTT AGAACTTCCG GCTTTTGCAT TAGCTTGTCT GATGCTCATG 
CCCCACTCAG AGAAAAGACA CCAGCAAATT AAGAATTTTC TGGGTTCCTG TGACCCTCAG 
GTTATTTTAA AGCAATTGGA AGAGCATATG AACACGGGCC AGCTAGCAGG ATTTTCACAT 
CAAATTAGAA GTCTGATTTT GAATAATATC ATCAATAAGA AGGAGTTTGG GATTTTGGCA 
AAGACCAAAT ACTTTCAAAT GTTGAAGATG CATGCGATGA ATACCAACAA TATCACTGAG 
CTAGTGAACT ATTTGGCAAA TGACTTAAGT TTAGATGAAG CTTCAGTCTT GATAACTGAA 
TATTCAAAGC ACTGCGGGAA ACCTGTGCCT CCAGACACTG CTCCCTGTGA AATTCTGAAG 
ATGTTTCTTA GTGGATTATC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875745.1
CDS	166..5049
Translation	MLESLLNSMSASVSLQKLCPWFKNDVIPFVRRTVPEGQIILAKWLEQAARNLELTDKANWPENGLQLAEIFFTAEKTDELGLASSWHWISLKDYQNTEEVCQLRTLVNNLRELITLHRKYNCKLALSDFEKENTTTIVFRMFDKVLAPELIPSILEKFIRVYMREHDLQEEELLLLYIEDLLNRCSSKSTSLFETAWEAKAMAVIACLSDTDLIFDAVLKIMYAAVVPWSAAVEQLVKQHLEMDHPKVKLLQESYKLMEMKKLLRGYGIREVNLLNKEIMRVVRYILKQDVPSSLEDALKVAQAFMLSDDEIYSLRIIDLIDREQGEDCLLLLKSLPPAEAEKTAERVIIWARLALQEEPDHSKEGKAWRMSVAKTSVDILKILCDIQKDNLQKKDECEEMLKLFKEVASLQENFEVFLSFEDYSNSSLVADLREQHIKAHEVAQAKHKPGSTPEPIAAEVRSPSMESKLHRQALALQMSKQELEAELTLRALKDGNIKTALKKCSDLFKYHCNADTGKLLFLTCQKLCQMLADNVPVTVPVGLNLPSMIHDLASQAATICSPDFLLDALELCKHTLMAVELSRQCQMDDCGILMKASFGTHKDPYEEWSYSDFFSEDGIVLESQMVLPVIYELISSLVPLAESKRYPLESTSLPYCSLNEGDGLVLPVINSISALLQNLQESSQWELALRFVVGSFGTCLQHSVSNFMNATLSEKLFGETTLVKSRHVVMELKEKAVIFIRENATTLLHKVFNCRLVDLDLALGYCTLLPQKDVFENLWKLIDKAWQNYDKILAISLVGSELASLYQEIEMGLKFRELSTDAQWGIRLGKLGISFQPVFRQHFLTKKDLIKALVENIDMDTSLILEYCSTFQLDCDAVLQLFIETLLHNTNAGQGQGDASMDSAKRRHPKLLAKALEMVPLLTSTKDLVISLSGILHKLDPYDYEMIEVVLKVIERADEKITNININQALSILKHLKSYRRISPPVDLEYQYMLEHVITLPSAAQTRLPFHLIFFGTAQNFWKILSTELSEESFPTLLLISKLMKFSLDTLYVSTAKHVFEKKLKPKLLKLTQAKSSTLINKEITKITQTIESCLLSIVNPEWAVAIAISLAQDIPEGSFKISALKFCLYLAERWLQNIPSQDEKREKAEALLKKLHIQYRRSGTEAVLIAHKLNTEEYLRVIGKPAHLIVSLYEHPSINQRIQNSSGTDYPDIHAAAKEIAEVNEINLEKVWDMLLEKWLCPSTKPGEKPSELFELQEDEALRRVQYLLLSRPIDYSSRMLFVFATSTTTTLGMHQLTFAHRTRALQCLFYLADKETIESLFKKPIEEVKSYLRCITFLASFETLNIPITYELFCSSPKEGMIKGLWKNHSHESMAVRLVTELCLEYKIYDLQLWNGLLQKLLGFNMIPYLRKVLKAISSIHSLWQVPYFSKAWQRVIQIPLLSASCPLSPDQLSDCSESLIAVLECPVSGDLDLIGVARQYIQLELPAFALACLMLMPHSEKRHQQIKNFLGSCDPQVILKQLEEHMNTGQLAGFSHQIRSLILNNIINKKEFGILAKTKYFQMLKMHAMNTNNITELVNYLANDLSLDEASVLITEYSKHCGKPVPPDTAPCEILKMFLSGLS

Target ORF information:

RefSeq Version	XM_017020256.2
Organism	Homo sapiens(human)
Definition	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020256.2

ORF Insert Sequence:

ATGCTGGAGA GCTTGCTCAA CTCAATGTCT GCATCAGTCT CTTTGCAAAA GCTGTGTCCA 
TGGTTTAAAA ATGATGTGAT TCCATTTGTA AGAAGGACTG TGCCTGAAGG ACAGATAATT 
CTTGCAAAAT GGTTGGAACA AGCAGCCAGG AACCTTGAAT TAACTGATAA GGCAAATTGG 
CCAGAAAATG GACTTCAATT GGCAGAGATA TTTTTTACAG CAGAAAAAAC AGACGAGTTG 
GGATTGGCAT CTTCCTGGCA TTGGATTTCC TTGAAAGATT ATCAGAACAC AGAGGAAGTA 
TGTCAGCTAA GGACTTTGGT AAATAACTTG CGAGAGTTGA TCACGTTGCA TAGGAAGTAC 
AACTGCAAAT TAGCCCTCTC TGATTTTGAG AAGGAAAATA CAACCACCAT AGTGTTCCGA 
ATGTTTGATA AAGTGCTGGC CCCAGAGCTT ATTCCCTCCA TCTTAGAGAA GTTTATAAGA 
GTTTACATGA GAGAACATGA CTTGCAAGAG GAGGAACTTC TCTTGCTGTA CATAGAGGAT 
TTACTGAATA GATGCAGCTC AAAGTCCACA TCACTCTTTG AAACAGCATG GGAAGCAAAG 
GCCATGGCAG TAATAGCGTG TTTATCTGAC ACGGACCTCA TATTTGATGC CGTGCTCAAG 
ATCATGTATG CGGCAGTGGT TCCTTGGAGT GCAGCTGTGG AGCAACTGGT GAAACAGCAC 
CTGGAAATGG ACCATCCCAA AGTCAAGTTA TTACAGGAAA GTTACAAACT AATGGAGATG 
AAAAAACTTT TACGAGGCTA TGGAATAAGA GAGGTAAATC TCTTAAACAA GGAAATAATG 
AGAGTGGTTA GATACATTCT CAAACAAGAT GTCCCATCTT CTTTAGAAGA TGCTTTAAAG 
GTAGCCCAAG CGTTTATGTT ATCTGATGAT GAGATCTACA GTCTAAGAAT TATTGACCTG 
ATTGATAGAG AACAGGGTGA AGACTGTCTC CTTCTGTTGA AGTCTTTGCC TCCTGCTGAA 
GCTGAGAAAA CTGCAGAAAG AGTCATCATA TGGGCACGAC TGGCATTACA AGAAGAGCCA 
GATCATTCTA AAGAGGGCAA GGCCTGGAGA ATGTCTGTAG CGAAGACATC CGTGGACATT 
CTTAAGATAC TATGTGACAT TCAGAAAGAC AATCTGCAGA AGAAGGACGA ATGTGAAGAA 
ATGTTGAAAC TATTTAAAGA GGTTGCTAGC TTACAGGAGA ACTTTGAGGT CTTTCTTTCA 
TTTGAAGATT ATAGCAATAG TTCCCTGGTA GCAGATCTCC GTGAGCAGCA CATTAAAGCT 
CACGAAGTTG CACAGGCGAA ACACAAACCT GGGAGCACCC CAGAGCCCAT AGCTGCTGAG 
GTGAGGAGCC CAAGCATGGA ATCAAAGCTG CACAGACAGG CACTGGCCCT GCAGATGTCC 
AAACAAGAGC TGGAGGCAGA GCTGACCTTG AGAGCCTTAA AAGATGGGAA CATCAAAACA 
GCACTGAAAA AATGCAGCGA CTTGTTTAAG TATCACTGCA ATGCTGACAC TGGGAAATTG 
CTATTTCTGA CATGTCAGAA GCTTTGTCAG ATGTTGGCTG ATAATGTCCC AGTGACAGTG 
CCTGTGGGAC TGAATCTTCC TTCCATGATA CATGATCTAG CAAGCCAAGC TGCCACCATT 
TGCAGTCCAG ATTTTTTACT AGATGCTTTA GAACTATGTA AACATACTTT AATGGCTGTA 
GAGCTTTCCA GACAATGCCA AATGGATGAC TGTGGAATCC TCATGAAAGC TTCTTTTGGG 
ACACATAAAG ATCCATATGA AGAGTGGTCT TACAGTGACT TCTTCAGTGA AGATGGAATT 
GTTCTTGAGT CACAGATGGT GCTTCCAGTG ATTTATGAAC TGATTTCATC TCTTGTGCCT 
CTAGCTGAAA GCAAGAGATA TCCCTTGGAG TCTACCAGTT TGCCATACTG CTCCCTTAAT 
GAAGGAGATG GCCTTGTTTT ACCTGTTATA AATTCCATCT CTGCCCTGCT TCAGAATCTT 
CAGGAATCTA GCCAGTGGGA GCTAGCCCTA AGATTTGTGG TTGGTTCATT TGGTACCTGT 
CTTCAGCACT CTGTGTCAAA CTTCATGAAT GCCACTTTGA GTGAAAAGTT ATTTGGAGAG 
ACTACATTAG TTAAATCAAG GCATGTTGTT ATGGAATTGA AAGAAAAAGC TGTTATATTT 
ATCAGGGAAA ATGCTACAAC ACTACTGCAC AAAGTATTTA ATTGTCGCTT GGTAGATCTT 
GACCTGGCGT TGGGTTACTG CACTCTCTTA CCTCAAAAAG ATGTGTTTGA AAATCTCTGG 
AAGCTCATAG ATAAAGCATG GCAGAATTAC GACAAAATCT TGGCAATATC TCTGGTGGGC 
TCTGAGCTGG CAAGTCTCTA TCAGGAAATA GAAATGGGGC TTAAGTTCCG TGAACTCAGT 
ACTGATGCCC AGTGGGGCAT TCGTCTTGGT AAACTTGGTA TTTCTTTTCA ACCAGTTTTC 
AGGCAACATT TTCTCACCAA GAAAGACCTC ATTAAAGCTC TTGTGGAGAA TATAGATATG 
GACACAAGCC TCATTTTGGA ATATTGCAGC ACATTTCAGT TGGACTGCGA TGCAGTTCTT 
CAGCTCTTCA TTGAAACGCT GCTCCACAAC ACAAATGCCG GCCAAGGCCA GGGAGATGCA 
AGCATGGACT CTGCAAAGCG GCGGCATCCC AAACTCCTGG CCAAAGCCCT TGAGATGGTT 
CCTTTACTGA CGAGCACAAA AGATTTGGTC ATCAGTCTTA GTGGAATACT ACATAAGTTG 
GATCCTTATG ACTATGAAAT GATTGAAGTT GTCTTGAAAG TTATAGAACG AGCTGATGAA 
AAGATAACCA ATATTAATAT TAATCAGGCA TTGAGTATTC TGAAACATTT GAAGTCATAC 
AGAAGAATTT CTCCTCCCGT GGATCTAGAA TATCAGTATA TGTTGGAACA TGTCATAACT 
TTGCCATCAG CTGCCCAAAC TAGACTGCCT TTTCACCTGA TATTCTTTGG CACAGCACAG 
AACTTCTGGA AAATTCTCTC TACAGAACTC AGTGAAGAAT CTTTCCCAAC ATTGCTCTTA 
ATTTCGAAAT TAATGAAGTT CTCTCTGGAC ACTCTGTACG TGTCTACAGC AAAACACGTT 
TTCGAAAAAA AACTGAAGCC AAAGCTCCTG AAGTTAACAC AAGCTAAATC CTCAACACTG 
ATTAACAAGG AAATAACTAA GATCACGCAG ACCATCGAAT CCTGCTTACT CTCTATAGTC 
AACCCAGAGT GGGCTGTAGC TATTGCCATC AGCCTTGCCC AGGATATCCC TGAAGGTTCC 
TTCAAGATAT CTGCTTTGAA ATTCTGCCTT TATTTAGCTG AGAGATGGCT ACAGAATATC 
CCATCGCAGG ACGAAAAACG TGAAAAAGCC GAGGCTTTGT TGAAGAAGCT TCATATCCAG 
TACCGGCGAT CGGGCACAGA AGCTGTGCTC ATAGCCCACA AGCTGAACAC TGAGGAATAT 
TTAAGAGTGA TCGGAAAGCC AGCACATCTT ATTGTCAGTC TCTACGAACA TCCTAGCATC 
AATCAAAGAA TTCAGAATTC ATCTGGCACA GATTATCCTG ATATTCATGC AGCAGCTAAA 
GAAATAGCCG AAGTCAATGA AATTAATTTG GAAAAAGTCT GGGACATGTT GTTGGAAAAA 
TGGCTATGCC CTTCAACAAA ACCTGGTGAA AAACCATCAG AATTATTTGA ACTTCAAGAA 
GATGAAGCCC TACGAAGAGT GCAGTATCTC CTCCTGTCTC GTCCAATTGA TTATAGTTCA 
AGAATGCTGT TTGTATTTGC AACATCAACT ACAACCACAT TAGGTATGCA TCAGTTAACT 
TTTGCCCATA GAACTCGAGC TCTTCAGTGT CTCTTCTATT TGGCTGACAA GGAAACTATA 
GAATCTCTCT TTAAAAAACC CATTGAAGAA GTGAAATCTT ATTTGAGATG TATAACTTTT 
CTGGCATCAT TTGAGACTTT GAATATCCCC ATCACATATG AATTATTTTG CAGCAGTCCT 
AAAGAAGGAA TGATTAAGGG TCTGTGGAAA AACCACAGCC ACGAGTCCAT GGCAGTAAGA 
TTGGTGACTG AGCTGTGTTT AGAATACAAA ATCTATGACC TGCAGCTTTG GAATGGACTC 
TTGCAAAAGC TTCTGGGCTT CAATATGATT CCTTATCTAA GGAAAGTTTT AAAAGCCATC 
TCCAGTATCC ATTCTTTATG GCAGGTTCCC TACTTCAGCA AAGCGTGGCA GCGTGTGATA 
CAGATACCAC TGCTTTCAGC CTCTTGTCCT TTAAGTCCTG ATCAGCTGTC AGATTGTTCT 
GAGAGTCTCA TCGCTGTCCT CGAATGTCCA GTCTCAGGTG ATCTTGACCT GATCGGAGTC 
GCCAGGCAGT ATATCCAGTT AGAACTTCCG GCTTTTGCAT TAGCTTGTCT GATGCTCATG 
CCCCACTCAG AGAAAAGACA CCAGCAAATT AAGAATTTTC TGGGTTCCTG TGACCCTCAG 
GTTATTTTAA AGCAATTGGA AGAGCATATG AACACGGGCC AGCTAGCAGG ATTTTCACAT 
CAAATTAGAA GTCTGATTTT GAATAATATC ATCAATAAGA AGGAGTTTGG GATTTTGGCA 
AAGACCAAAT ACTTTCAAAT GTTGAAGATG CATGCGATGA ATACCAACAA TATCACTGAG 
CTAGTGAACT ATTTGGCAAA TGACTTAAGT TTAGATGAAG CTTCAGTCTT GATAACTGAA 
TATTCAAAGC ACTGCGGGAA ACCTGTGCCT CCAGACACTG CTCCCTGTGA AATTCTGAAG 
ATGTTTCTTA GTGGATTATC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNTC1 ( XM_017020256.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875745.1 Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X6, mRNA.

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CloneID	OHu84342
Clone ID Related Accession (Same CDS sequence)	XM_017020256.2 , XM_017020256.1
Accession Version	XM_017020256.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	kinetochore-associated protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAGA GCTTGCTCAA CTCAATGTCT GCATCAGTCT CTTTGCAAAA GCTGTGTCCA 
TGGTTTAAAA ATGATGTGAT TCCATTTGTA AGAAGGACTG TGCCTGAAGG ACAGATAATT 
CTTGCAAAAT GGTTGGAACA AGCAGCCAGG AACCTTGAAT TAACTGATAA GGCAAATTGG 
CCAGAAAATG GACTTCAATT GGCAGAGATA TTTTTTACAG CAGAAAAAAC AGACGAGTTG 
GGATTGGCAT CTTCCTGGCA TTGGATTTCC TTGAAAGATT ATCAGAACAC AGAGGAAGTA 
TGTCAGCTAA GGACTTTGGT AAATAACTTG CGAGAGTTGA TCACGTTGCA TAGGAAGTAC 
AACTGCAAAT TAGCCCTCTC TGATTTTGAG AAGGAAAATA CAACCACCAT AGTGTTCCGA 
ATGTTTGATA AAGTGCTGGC CCCAGAGCTT ATTCCCTCCA TCTTAGAGAA GTTTATAAGA 
GTTTACATGA GAGAACATGA CTTGCAAGAG GAGGAACTTC TCTTGCTGTA CATAGAGGAT 
TTACTGAATA GATGCAGCTC AAAGTCCACA TCACTCTTTG AAACAGCATG GGAAGCAAAG 
GCCATGGCAG TAATAGCGTG TTTATCTGAC ACGGACCTCA TATTTGATGC CGTGCTCAAG 
ATCATGTATG CGGCAGTGGT TCCTTGGAGT GCAGCTGTGG AGCAACTGGT GAAACAGCAC 
CTGGAAATGG ACCATCCCAA AGTCAAGTTA TTACAGGAAA GTTACAAACT AATGGAGATG 
AAAAAACTTT TACGAGGCTA TGGAATAAGA GAGGTAAATC TCTTAAACAA GGAAATAATG 
AGAGTGGTTA GATACATTCT CAAACAAGAT GTCCCATCTT CTTTAGAAGA TGCTTTAAAG 
GTAGCCCAAG CGTTTATGTT ATCTGATGAT GAGATCTACA GTCTAAGAAT TATTGACCTG 
ATTGATAGAG AACAGGGTGA AGACTGTCTC CTTCTGTTGA AGTCTTTGCC TCCTGCTGAA 
GCTGAGAAAA CTGCAGAAAG AGTCATCATA TGGGCACGAC TGGCATTACA AGAAGAGCCA 
GATCATTCTA AAGAGGGCAA GGCCTGGAGA ATGTCTGTAG CGAAGACATC CGTGGACATT 
CTTAAGATAC TATGTGACAT TCAGAAAGAC AATCTGCAGA AGAAGGACGA ATGTGAAGAA 
ATGTTGAAAC TATTTAAAGA GGTTGCTAGC TTACAGGAGA ACTTTGAGGT CTTTCTTTCA 
TTTGAAGATT ATAGCAATAG TTCCCTGGTA GCAGATCTCC GTGAGCAGCA CATTAAAGCT 
CACGAAGTTG CACAGGCGAA ACACAAACCT GGGAGCACCC CAGAGCCCAT AGCTGCTGAG 
GTGAGGAGCC CAAGCATGGA ATCAAAGCTG CACAGACAGG CACTGGCCCT GCAGATGTCC 
AAACAAGAGC TGGAGGCAGA GCTGACCTTG AGAGCCTTAA AAGATGGGAA CATCAAAACA 
GCACTGAAAA AATGCAGCGA CTTGTTTAAG TATCACTGCA ATGCTGACAC TGGGAAATTG 
CTATTTCTGA CATGTCAGAA GCTTTGTCAG ATGTTGGCTG ATAATGTCCC AGTGACAGTG 
CCTGTGGGAC TGAATCTTCC TTCCATGATA CATGATCTAG CAAGCCAAGC TGCCACCATT 
TGCAGTCCAG ATTTTTTACT AGATGCTTTA GAACTATGTA AACATACTTT AATGGCTGTA 
GAGCTTTCCA GACAATGCCA AATGGATGAC TGTGGAATCC TCATGAAAGC TTCTTTTGGG 
ACACATAAAG ATCCATATGA AGAGTGGTCT TACAGTGACT TCTTCAGTGA AGATGGAATT 
GTTCTTGAGT CACAGATGGT GCTTCCAGTG ATTTATGAAC TGATTTCATC TCTTGTGCCT 
CTAGCTGAAA GCAAGAGATA TCCCTTGGAG TCTACCAGTT TGCCATACTG CTCCCTTAAT 
GAAGGAGATG GCCTTGTTTT ACCTGTTATA AATTCCATCT CTGCCCTGCT TCAGAATCTT 
CAGGAATCTA GCCAGTGGGA GCTAGCCCTA AGATTTGTGG TTGGTTCATT TGGTACCTGT 
CTTCAGCACT CTGTGTCAAA CTTCATGAAT GCCACTTTGA GTGAAAAGTT ATTTGGAGAG 
ACTACATTAG TTAAATCAAG GCATGTTGTT ATGGAATTGA AAGAAAAAGC TGTTATATTT 
ATCAGGGAAA ATGCTACAAC ACTACTGCAC AAAGTATTTA ATTGTCGCTT GGTAGATCTT 
GACCTGGCGT TGGGTTACTG CACTCTCTTA CCTCAAAAAG ATGTGTTTGA AAATCTCTGG 
AAGCTCATAG ATAAAGCATG GCAGAATTAC GACAAAATCT TGGCAATATC TCTGGTGGGC 
TCTGAGCTGG CAAGTCTCTA TCAGGAAATA GAAATGGGGC TTAAGTTCCG TGAACTCAGT 
ACTGATGCCC AGTGGGGCAT TCGTCTTGGT AAACTTGGTA TTTCTTTTCA ACCAGTTTTC 
AGGCAACATT TTCTCACCAA GAAAGACCTC ATTAAAGCTC TTGTGGAGAA TATAGATATG 
GACACAAGCC TCATTTTGGA ATATTGCAGC ACATTTCAGT TGGACTGCGA TGCAGTTCTT 
CAGCTCTTCA TTGAAACGCT GCTCCACAAC ACAAATGCCG GCCAAGGCCA GGGAGATGCA 
AGCATGGACT CTGCAAAGCG GCGGCATCCC AAACTCCTGG CCAAAGCCCT TGAGATGGTT 
CCTTTACTGA CGAGCACAAA AGATTTGGTC ATCAGTCTTA GTGGAATACT ACATAAGTTG 
GATCCTTATG ACTATGAAAT GATTGAAGTT GTCTTGAAAG TTATAGAACG AGCTGATGAA 
AAGATAACCA ATATTAATAT TAATCAGGCA TTGAGTATTC TGAAACATTT GAAGTCATAC 
AGAAGAATTT CTCCTCCCGT GGATCTAGAA TATCAGTATA TGTTGGAACA TGTCATAACT 
TTGCCATCAG CTGCCCAAAC TAGACTGCCT TTTCACCTGA TATTCTTTGG CACAGCACAG 
AACTTCTGGA AAATTCTCTC TACAGAACTC AGTGAAGAAT CTTTCCCAAC ATTGCTCTTA 
ATTTCGAAAT TAATGAAGTT CTCTCTGGAC ACTCTGTACG TGTCTACAGC AAAACACGTT 
TTCGAAAAAA AACTGAAGCC AAAGCTCCTG AAGTTAACAC AAGCTAAATC CTCAACACTG 
ATTAACAAGG AAATAACTAA GATCACGCAG ACCATCGAAT CCTGCTTACT CTCTATAGTC 
AACCCAGAGT GGGCTGTAGC TATTGCCATC AGCCTTGCCC AGGATATCCC TGAAGGTTCC 
TTCAAGATAT CTGCTTTGAA ATTCTGCCTT TATTTAGCTG AGAGATGGCT ACAGAATATC 
CCATCGCAGG ACGAAAAACG TGAAAAAGCC GAGGCTTTGT TGAAGAAGCT TCATATCCAG 
TACCGGCGAT CGGGCACAGA AGCTGTGCTC ATAGCCCACA AGCTGAACAC TGAGGAATAT 
TTAAGAGTGA TCGGAAAGCC AGCACATCTT ATTGTCAGTC TCTACGAACA TCCTAGCATC 
AATCAAAGAA TTCAGAATTC ATCTGGCACA GATTATCCTG ATATTCATGC AGCAGCTAAA 
GAAATAGCCG AAGTCAATGA AATTAATTTG GAAAAAGTCT GGGACATGTT GTTGGAAAAA 
TGGCTATGCC CTTCAACAAA ACCTGGTGAA AAACCATCAG AATTATTTGA ACTTCAAGAA 
GATGAAGCCC TACGAAGAGT GCAGTATCTC CTCCTGTCTC GTCCAATTGA TTATAGTTCA 
AGAATGCTGT TTGTATTTGC AACATCAACT ACAACCACAT TAGGTATGCA TCAGTTAACT 
TTTGCCCATA GAACTCGAGC TCTTCAGTGT CTCTTCTATT TGGCTGACAA GGAAACTATA 
GAATCTCTCT TTAAAAAACC CATTGAAGAA GTGAAATCTT ATTTGAGATG TATAACTTTT 
CTGGCATCAT TTGAGACTTT GAATATCCCC ATCACATATG AATTATTTTG CAGCAGTCCT 
AAAGAAGGAA TGATTAAGGG TCTGTGGAAA AACCACAGCC ACGAGTCCAT GGCAGTAAGA 
TTGGTGACTG AGCTGTGTTT AGAATACAAA ATCTATGACC TGCAGCTTTG GAATGGACTC 
TTGCAAAAGC TTCTGGGCTT CAATATGATT CCTTATCTAA GGAAAGTTTT AAAAGCCATC 
TCCAGTATCC ATTCTTTATG GCAGGTTCCC TACTTCAGCA AAGCGTGGCA GCGTGTGATA 
CAGATACCAC TGCTTTCAGC CTCTTGTCCT TTAAGTCCTG ATCAGCTGTC AGATTGTTCT 
GAGAGTCTCA TCGCTGTCCT CGAATGTCCA GTCTCAGGTG ATCTTGACCT GATCGGAGTC 
GCCAGGCAGT ATATCCAGTT AGAACTTCCG GCTTTTGCAT TAGCTTGTCT GATGCTCATG 
CCCCACTCAG AGAAAAGACA CCAGCAAATT AAGAATTTTC TGGGTTCCTG TGACCCTCAG 
GTTATTTTAA AGCAATTGGA AGAGCATATG AACACGGGCC AGCTAGCAGG ATTTTCACAT 
CAAATTAGAA GTCTGATTTT GAATAATATC ATCAATAAGA AGGAGTTTGG GATTTTGGCA 
AAGACCAAAT ACTTTCAAAT GTTGAAGATG CATGCGATGA ATACCAACAA TATCACTGAG 
CTAGTGAACT ATTTGGCAAA TGACTTAAGT TTAGATGAAG CTTCAGTCTT GATAACTGAA 
TATTCAAAGC ACTGCGGGAA ACCTGTGCCT CCAGACACTG CTCCCTGTGA AATTCTGAAG 
ATGTTTCTTA GTGGATTATC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875745.1
CDS	166..5049
Translation	MLESLLNSMSASVSLQKLCPWFKNDVIPFVRRTVPEGQIILAKWLEQAARNLELTDKANWPENGLQLAEIFFTAEKTDELGLASSWHWISLKDYQNTEEVCQLRTLVNNLRELITLHRKYNCKLALSDFEKENTTTIVFRMFDKVLAPELIPSILEKFIRVYMREHDLQEEELLLLYIEDLLNRCSSKSTSLFETAWEAKAMAVIACLSDTDLIFDAVLKIMYAAVVPWSAAVEQLVKQHLEMDHPKVKLLQESYKLMEMKKLLRGYGIREVNLLNKEIMRVVRYILKQDVPSSLEDALKVAQAFMLSDDEIYSLRIIDLIDREQGEDCLLLLKSLPPAEAEKTAERVIIWARLALQEEPDHSKEGKAWRMSVAKTSVDILKILCDIQKDNLQKKDECEEMLKLFKEVASLQENFEVFLSFEDYSNSSLVADLREQHIKAHEVAQAKHKPGSTPEPIAAEVRSPSMESKLHRQALALQMSKQELEAELTLRALKDGNIKTALKKCSDLFKYHCNADTGKLLFLTCQKLCQMLADNVPVTVPVGLNLPSMIHDLASQAATICSPDFLLDALELCKHTLMAVELSRQCQMDDCGILMKASFGTHKDPYEEWSYSDFFSEDGIVLESQMVLPVIYELISSLVPLAESKRYPLESTSLPYCSLNEGDGLVLPVINSISALLQNLQESSQWELALRFVVGSFGTCLQHSVSNFMNATLSEKLFGETTLVKSRHVVMELKEKAVIFIRENATTLLHKVFNCRLVDLDLALGYCTLLPQKDVFENLWKLIDKAWQNYDKILAISLVGSELASLYQEIEMGLKFRELSTDAQWGIRLGKLGISFQPVFRQHFLTKKDLIKALVENIDMDTSLILEYCSTFQLDCDAVLQLFIETLLHNTNAGQGQGDASMDSAKRRHPKLLAKALEMVPLLTSTKDLVISLSGILHKLDPYDYEMIEVVLKVIERADEKITNININQALSILKHLKSYRRISPPVDLEYQYMLEHVITLPSAAQTRLPFHLIFFGTAQNFWKILSTELSEESFPTLLLISKLMKFSLDTLYVSTAKHVFEKKLKPKLLKLTQAKSSTLINKEITKITQTIESCLLSIVNPEWAVAIAISLAQDIPEGSFKISALKFCLYLAERWLQNIPSQDEKREKAEALLKKLHIQYRRSGTEAVLIAHKLNTEEYLRVIGKPAHLIVSLYEHPSINQRIQNSSGTDYPDIHAAAKEIAEVNEINLEKVWDMLLEKWLCPSTKPGEKPSELFELQEDEALRRVQYLLLSRPIDYSSRMLFVFATSTTTTLGMHQLTFAHRTRALQCLFYLADKETIESLFKKPIEEVKSYLRCITFLASFETLNIPITYELFCSSPKEGMIKGLWKNHSHESMAVRLVTELCLEYKIYDLQLWNGLLQKLLGFNMIPYLRKVLKAISSIHSLWQVPYFSKAWQRVIQIPLLSASCPLSPDQLSDCSESLIAVLECPVSGDLDLIGVARQYIQLELPAFALACLMLMPHSEKRHQQIKNFLGSCDPQVILKQLEEHMNTGQLAGFSHQIRSLILNNIINKKEFGILAKTKYFQMLKMHAMNTNNITELVNYLANDLSLDEASVLITEYSKHCGKPVPPDTAPCEILKMFLSGLS

Target ORF information:

RefSeq Version	XM_017020256.1
Organism	Homo sapiens(human)
Definition	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020256.1

ORF Insert Sequence:

ATGCTGGAGA GCTTGCTCAA CTCAATGTCT GCATCAGTCT CTTTGCAAAA GCTGTGTCCA 
TGGTTTAAAA ATGATGTGAT TCCATTTGTA AGAAGGACTG TGCCTGAAGG ACAGATAATT 
CTTGCAAAAT GGTTGGAACA AGCAGCCAGG AACCTTGAAT TAACTGATAA GGCAAATTGG 
CCAGAAAATG GACTTCAATT GGCAGAGATA TTTTTTACAG CAGAAAAAAC AGACGAGTTG 
GGATTGGCAT CTTCCTGGCA TTGGATTTCC TTGAAAGATT ATCAGAACAC AGAGGAAGTA 
TGTCAGCTAA GGACTTTGGT AAATAACTTG CGAGAGTTGA TCACGTTGCA TAGGAAGTAC 
AACTGCAAAT TAGCCCTCTC TGATTTTGAG AAGGAAAATA CAACCACCAT AGTGTTCCGA 
ATGTTTGATA AAGTGCTGGC CCCAGAGCTT ATTCCCTCCA TCTTAGAGAA GTTTATAAGA 
GTTTACATGA GAGAACATGA CTTGCAAGAG GAGGAACTTC TCTTGCTGTA CATAGAGGAT 
TTACTGAATA GATGCAGCTC AAAGTCCACA TCACTCTTTG AAACAGCATG GGAAGCAAAG 
GCCATGGCAG TAATAGCGTG TTTATCTGAC ACGGACCTCA TATTTGATGC CGTGCTCAAG 
ATCATGTATG CGGCAGTGGT TCCTTGGAGT GCAGCTGTGG AGCAACTGGT GAAACAGCAC 
CTGGAAATGG ACCATCCCAA AGTCAAGTTA TTACAGGAAA GTTACAAACT AATGGAGATG 
AAAAAACTTT TACGAGGCTA TGGAATAAGA GAGGTAAATC TCTTAAACAA GGAAATAATG 
AGAGTGGTTA GATACATTCT CAAACAAGAT GTCCCATCTT CTTTAGAAGA TGCTTTAAAG 
GTAGCCCAAG CGTTTATGTT ATCTGATGAT GAGATCTACA GTCTAAGAAT TATTGACCTG 
ATTGATAGAG AACAGGGTGA AGACTGTCTC CTTCTGTTGA AGTCTTTGCC TCCTGCTGAA 
GCTGAGAAAA CTGCAGAAAG AGTCATCATA TGGGCACGAC TGGCATTACA AGAAGAGCCA 
GATCATTCTA AAGAGGGCAA GGCCTGGAGA ATGTCTGTAG CGAAGACATC CGTGGACATT 
CTTAAGATAC TATGTGACAT TCAGAAAGAC AATCTGCAGA AGAAGGACGA ATGTGAAGAA 
ATGTTGAAAC TATTTAAAGA GGTTGCTAGC TTACAGGAGA ACTTTGAGGT CTTTCTTTCA 
TTTGAAGATT ATAGCAATAG TTCCCTGGTA GCAGATCTCC GTGAGCAGCA CATTAAAGCT 
CACGAAGTTG CACAGGCGAA ACACAAACCT GGGAGCACCC CAGAGCCCAT AGCTGCTGAG 
GTGAGGAGCC CAAGCATGGA ATCAAAGCTG CACAGACAGG CACTGGCCCT GCAGATGTCC 
AAACAAGAGC TGGAGGCAGA GCTGACCTTG AGAGCCTTAA AAGATGGGAA CATCAAAACA 
GCACTGAAAA AATGCAGCGA CTTGTTTAAG TATCACTGCA ATGCTGACAC TGGGAAATTG 
CTATTTCTGA CATGTCAGAA GCTTTGTCAG ATGTTGGCTG ATAATGTCCC AGTGACAGTG 
CCTGTGGGAC TGAATCTTCC TTCCATGATA CATGATCTAG CAAGCCAAGC TGCCACCATT 
TGCAGTCCAG ATTTTTTACT AGATGCTTTA GAACTATGTA AACATACTTT AATGGCTGTA 
GAGCTTTCCA GACAATGCCA AATGGATGAC TGTGGAATCC TCATGAAAGC TTCTTTTGGG 
ACACATAAAG ATCCATATGA AGAGTGGTCT TACAGTGACT TCTTCAGTGA AGATGGAATT 
GTTCTTGAGT CACAGATGGT GCTTCCAGTG ATTTATGAAC TGATTTCATC TCTTGTGCCT 
CTAGCTGAAA GCAAGAGATA TCCCTTGGAG TCTACCAGTT TGCCATACTG CTCCCTTAAT 
GAAGGAGATG GCCTTGTTTT ACCTGTTATA AATTCCATCT CTGCCCTGCT TCAGAATCTT 
CAGGAATCTA GCCAGTGGGA GCTAGCCCTA AGATTTGTGG TTGGTTCATT TGGTACCTGT 
CTTCAGCACT CTGTGTCAAA CTTCATGAAT GCCACTTTGA GTGAAAAGTT ATTTGGAGAG 
ACTACATTAG TTAAATCAAG GCATGTTGTT ATGGAATTGA AAGAAAAAGC TGTTATATTT 
ATCAGGGAAA ATGCTACAAC ACTACTGCAC AAAGTATTTA ATTGTCGCTT GGTAGATCTT 
GACCTGGCGT TGGGTTACTG CACTCTCTTA CCTCAAAAAG ATGTGTTTGA AAATCTCTGG 
AAGCTCATAG ATAAAGCATG GCAGAATTAC GACAAAATCT TGGCAATATC TCTGGTGGGC 
TCTGAGCTGG CAAGTCTCTA TCAGGAAATA GAAATGGGGC TTAAGTTCCG TGAACTCAGT 
ACTGATGCCC AGTGGGGCAT TCGTCTTGGT AAACTTGGTA TTTCTTTTCA ACCAGTTTTC 
AGGCAACATT TTCTCACCAA GAAAGACCTC ATTAAAGCTC TTGTGGAGAA TATAGATATG 
GACACAAGCC TCATTTTGGA ATATTGCAGC ACATTTCAGT TGGACTGCGA TGCAGTTCTT 
CAGCTCTTCA TTGAAACGCT GCTCCACAAC ACAAATGCCG GCCAAGGCCA GGGAGATGCA 
AGCATGGACT CTGCAAAGCG GCGGCATCCC AAACTCCTGG CCAAAGCCCT TGAGATGGTT 
CCTTTACTGA CGAGCACAAA AGATTTGGTC ATCAGTCTTA GTGGAATACT ACATAAGTTG 
GATCCTTATG ACTATGAAAT GATTGAAGTT GTCTTGAAAG TTATAGAACG AGCTGATGAA 
AAGATAACCA ATATTAATAT TAATCAGGCA TTGAGTATTC TGAAACATTT GAAGTCATAC 
AGAAGAATTT CTCCTCCCGT GGATCTAGAA TATCAGTATA TGTTGGAACA TGTCATAACT 
TTGCCATCAG CTGCCCAAAC TAGACTGCCT TTTCACCTGA TATTCTTTGG CACAGCACAG 
AACTTCTGGA AAATTCTCTC TACAGAACTC AGTGAAGAAT CTTTCCCAAC ATTGCTCTTA 
ATTTCGAAAT TAATGAAGTT CTCTCTGGAC ACTCTGTACG TGTCTACAGC AAAACACGTT 
TTCGAAAAAA AACTGAAGCC AAAGCTCCTG AAGTTAACAC AAGCTAAATC CTCAACACTG 
ATTAACAAGG AAATAACTAA GATCACGCAG ACCATCGAAT CCTGCTTACT CTCTATAGTC 
AACCCAGAGT GGGCTGTAGC TATTGCCATC AGCCTTGCCC AGGATATCCC TGAAGGTTCC 
TTCAAGATAT CTGCTTTGAA ATTCTGCCTT TATTTAGCTG AGAGATGGCT ACAGAATATC 
CCATCGCAGG ACGAAAAACG TGAAAAAGCC GAGGCTTTGT TGAAGAAGCT TCATATCCAG 
TACCGGCGAT CGGGCACAGA AGCTGTGCTC ATAGCCCACA AGCTGAACAC TGAGGAATAT 
TTAAGAGTGA TCGGAAAGCC AGCACATCTT ATTGTCAGTC TCTACGAACA TCCTAGCATC 
AATCAAAGAA TTCAGAATTC ATCTGGCACA GATTATCCTG ATATTCATGC AGCAGCTAAA 
GAAATAGCCG AAGTCAATGA AATTAATTTG GAAAAAGTCT GGGACATGTT GTTGGAAAAA 
TGGCTATGCC CTTCAACAAA ACCTGGTGAA AAACCATCAG AATTATTTGA ACTTCAAGAA 
GATGAAGCCC TACGAAGAGT GCAGTATCTC CTCCTGTCTC GTCCAATTGA TTATAGTTCA 
AGAATGCTGT TTGTATTTGC AACATCAACT ACAACCACAT TAGGTATGCA TCAGTTAACT 
TTTGCCCATA GAACTCGAGC TCTTCAGTGT CTCTTCTATT TGGCTGACAA GGAAACTATA 
GAATCTCTCT TTAAAAAACC CATTGAAGAA GTGAAATCTT ATTTGAGATG TATAACTTTT 
CTGGCATCAT TTGAGACTTT GAATATCCCC ATCACATATG AATTATTTTG CAGCAGTCCT 
AAAGAAGGAA TGATTAAGGG TCTGTGGAAA AACCACAGCC ACGAGTCCAT GGCAGTAAGA 
TTGGTGACTG AGCTGTGTTT AGAATACAAA ATCTATGACC TGCAGCTTTG GAATGGACTC 
TTGCAAAAGC TTCTGGGCTT CAATATGATT CCTTATCTAA GGAAAGTTTT AAAAGCCATC 
TCCAGTATCC ATTCTTTATG GCAGGTTCCC TACTTCAGCA AAGCGTGGCA GCGTGTGATA 
CAGATACCAC TGCTTTCAGC CTCTTGTCCT TTAAGTCCTG ATCAGCTGTC AGATTGTTCT 
GAGAGTCTCA TCGCTGTCCT CGAATGTCCA GTCTCAGGTG ATCTTGACCT GATCGGAGTC 
GCCAGGCAGT ATATCCAGTT AGAACTTCCG GCTTTTGCAT TAGCTTGTCT GATGCTCATG 
CCCCACTCAG AGAAAAGACA CCAGCAAATT AAGAATTTTC TGGGTTCCTG TGACCCTCAG 
GTTATTTTAA AGCAATTGGA AGAGCATATG AACACGGGCC AGCTAGCAGG ATTTTCACAT 
CAAATTAGAA GTCTGATTTT GAATAATATC ATCAATAAGA AGGAGTTTGG GATTTTGGCA 
AAGACCAAAT ACTTTCAAAT GTTGAAGATG CATGCGATGA ATACCAACAA TATCACTGAG 
CTAGTGAACT ATTTGGCAAA TGACTTAAGT TTAGATGAAG CTTCAGTCTT GATAACTGAA 
TATTCAAAGC ACTGCGGGAA ACCTGTGCCT CCAGACACTG CTCCCTGTGA AATTCTGAAG 
ATGTTTCTTA GTGGATTATC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNTC1 ( XM_011539029.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537331.1 Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X5, mRNA.

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CloneID	OHu63243
Clone ID Related Accession (Same CDS sequence)	XM_011539029.3 , XM_011539029.2
Accession Version	XM_011539029.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	kinetochore-associated protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011539029.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAACCA 
GTTTTCAGGC AACATTTTCT CACCAAGAAA GACCTCATTA AAGCTCTTGT GGAGAATATA 
GATATGGACA CAAGCCTCAT TTTGGAATAT TGCAGCACAT TTCAGTTGGA CTGCGATGCA 
GTTCTTCAGC TCTTCATTGA AACGCTGCTC CACAACACAA ATGCCGGCCA AGGCCAGGGA 
GATGCAAGCA TGGACTCTGC AAAGCGGCGG CATCCCAAAC TCCTGGCCAA AGCCCTTGAG 
ATGGTTCCTT TACTGACGAG CACAAAAGAT TTGGTCATCA GTCTTAGTGG AATACTACAT 
AAGTTGGATC CTTATGACTA TGAAATGATT GAAGTTGTCT TGAAAGTTAT AGAACGAGCT 
GATGAAAAGA TAACCAATAT TAATATTAAT CAGGTGATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537331.1
CDS	146..4807
Translation	MWNDIELLTNDDTGSGYLSVGSRKEHGTALYQVDLLVKISSEKASLNPKIQACSLSDGFIIVADQSVILLDSICRSLQLHLVFDTEVDVVGLCQEGKFLLVGERSGNLHLIHVTSKQTLLTNAFVQKANDENRRTYQNLVIEKDGSNEGTYYMLLLTYSGFFCITNLQLLKIQQAIENVDFSTAKKLQGQIKSSFISTENYHTLGCLSLVAGDLASEVPVIIGGTGNCAFSKWEPDSSKKGMTVKNLIDAEIIKGAKKFQLIDNLLFVLDTDNVLSLWDIYTLTPVWNWPSLHVEEFLLTTEADSPSSVTWQGITNLKLIALTASANKKMKNLMVYSLPTMEILYSLEVSSVSSLVQTGISTDTIYLLEGVCKNDPKLSEDSVSVLVLRCLTEALPENRLSRLLHKHRFAEAESFAIQFGLDVELVYKVKSNHILEKLALSSVDASEQTEWQQLVDDAKENLHKIQDDEFVVNYCLKAQWITYETTQEMLNYAKTRLLKKEDKTALIYSDGLKEVLRAHAKLTTFYGAFGPEKFSGSSWIEFLNNEDDLKDIFLQLKEGNLVCAQYLWLRHRANFESRFDVKMLESLLNSMSASVSLQKLCPWFKNDVIPFVRRTVPEGQIILAKWLEQAARNLELTDKANWPENGLQLAEIFFTAEKTDELGLASSWHWISLKDYQNTEEVCQLRTLVNNLRELITLHRKYNCKLALSDFEKENTTTIVFRMFDKVLAPELIPSILEKFIRVYMREHDLQEEELLLLYIEDLLNRCSSKSTSLFETAWEAKAMAVIACLSDTDLIFDAVLKIMYAAVVPWSAAVEQLVKQHLEMDHPKVKLLQESYKLMEMKKLLRGYGIREVNLLNKEIMRVVRYILKQDVPSSLEDALKVAQAFMLSDDEIYSLRIIDLIDREQGEDCLLLLKSLPPAEAEKTAERVIIWARLALQEEPDHSKEGKAWRMSVAKTSVDILKILCDIQKDNLQKKDECEEMLKLFKEVASLQENFEVFLSFEDYSNSSLVADLREQHIKAHEVAQAKHKPGSTPEPIAAEVRSPSMESKLHRQALALQMSKQELEAELTLRALKDGNIKTALKKCSDLFKYHCNADTGKLLFLTCQKLCQMLADNVPVTVPVGLNLPSMIHDLASQAATICSPDFLLDALELCKHTLMAVELSRQCQMDDCGILMKASFGTHKDPYEEWSYSDFFSEDGIVLESQMVLPVIYELISSLVPLAESKRYPLESTSLPYCSLNEGDGLVLPVINSISALLQNLQESSQWELALRFVVGSFGTCLQHSVSNFMNATLSEKLFGETTLVKSRHVVMELKEKAVIFIRENATTLLHKVFNCRLVDLDLALGYCTLLPQKDVFENLWKLIDKAWQNYDKILAISLVGSELASLYQEIEMGLKFRELSTDAQWGIRLGKLGISFQPVFRQHFLTKKDLIKALVENIDMDTSLILEYCSTFQLDCDAVLQLFIETLLHNTNAGQGQGDASMDSAKRRHPKLLAKALEMVPLLTSTKDLVISLSGILHKLDPYDYEMIEVVLKVIERADEKITNININQVI

Target ORF information:

RefSeq Version	XM_011539029.3
Organism	Homo sapiens(human)
Definition	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539029.3

ORF Insert Sequence:

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAACCA 
GTTTTCAGGC AACATTTTCT CACCAAGAAA GACCTCATTA AAGCTCTTGT GGAGAATATA 
GATATGGACA CAAGCCTCAT TTTGGAATAT TGCAGCACAT TTCAGTTGGA CTGCGATGCA 
GTTCTTCAGC TCTTCATTGA AACGCTGCTC CACAACACAA ATGCCGGCCA AGGCCAGGGA 
GATGCAAGCA TGGACTCTGC AAAGCGGCGG CATCCCAAAC TCCTGGCCAA AGCCCTTGAG 
ATGGTTCCTT TACTGACGAG CACAAAAGAT TTGGTCATCA GTCTTAGTGG AATACTACAT 
AAGTTGGATC CTTATGACTA TGAAATGATT GAAGTTGTCT TGAAAGTTAT AGAACGAGCT 
GATGAAAAGA TAACCAATAT TAATATTAAT CAGGTGATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNTC1 ( XM_011539029.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537331.1 Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X5, mRNA.

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CloneID	OHu63243
Clone ID Related Accession (Same CDS sequence)	XM_011539029.3 , XM_011539029.2
Accession Version	XM_011539029.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	kinetochore-associated protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011539029.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAACCA 
GTTTTCAGGC AACATTTTCT CACCAAGAAA GACCTCATTA AAGCTCTTGT GGAGAATATA 
GATATGGACA CAAGCCTCAT TTTGGAATAT TGCAGCACAT TTCAGTTGGA CTGCGATGCA 
GTTCTTCAGC TCTTCATTGA AACGCTGCTC CACAACACAA ATGCCGGCCA AGGCCAGGGA 
GATGCAAGCA TGGACTCTGC AAAGCGGCGG CATCCCAAAC TCCTGGCCAA AGCCCTTGAG 
ATGGTTCCTT TACTGACGAG CACAAAAGAT TTGGTCATCA GTCTTAGTGG AATACTACAT 
AAGTTGGATC CTTATGACTA TGAAATGATT GAAGTTGTCT TGAAAGTTAT AGAACGAGCT 
GATGAAAAGA TAACCAATAT TAATATTAAT CAGGTGATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537331.1
CDS	146..4807
Translation	MWNDIELLTNDDTGSGYLSVGSRKEHGTALYQVDLLVKISSEKASLNPKIQACSLSDGFIIVADQSVILLDSICRSLQLHLVFDTEVDVVGLCQEGKFLLVGERSGNLHLIHVTSKQTLLTNAFVQKANDENRRTYQNLVIEKDGSNEGTYYMLLLTYSGFFCITNLQLLKIQQAIENVDFSTAKKLQGQIKSSFISTENYHTLGCLSLVAGDLASEVPVIIGGTGNCAFSKWEPDSSKKGMTVKNLIDAEIIKGAKKFQLIDNLLFVLDTDNVLSLWDIYTLTPVWNWPSLHVEEFLLTTEADSPSSVTWQGITNLKLIALTASANKKMKNLMVYSLPTMEILYSLEVSSVSSLVQTGISTDTIYLLEGVCKNDPKLSEDSVSVLVLRCLTEALPENRLSRLLHKHRFAEAESFAIQFGLDVELVYKVKSNHILEKLALSSVDASEQTEWQQLVDDAKENLHKIQDDEFVVNYCLKAQWITYETTQEMLNYAKTRLLKKEDKTALIYSDGLKEVLRAHAKLTTFYGAFGPEKFSGSSWIEFLNNEDDLKDIFLQLKEGNLVCAQYLWLRHRANFESRFDVKMLESLLNSMSASVSLQKLCPWFKNDVIPFVRRTVPEGQIILAKWLEQAARNLELTDKANWPENGLQLAEIFFTAEKTDELGLASSWHWISLKDYQNTEEVCQLRTLVNNLRELITLHRKYNCKLALSDFEKENTTTIVFRMFDKVLAPELIPSILEKFIRVYMREHDLQEEELLLLYIEDLLNRCSSKSTSLFETAWEAKAMAVIACLSDTDLIFDAVLKIMYAAVVPWSAAVEQLVKQHLEMDHPKVKLLQESYKLMEMKKLLRGYGIREVNLLNKEIMRVVRYILKQDVPSSLEDALKVAQAFMLSDDEIYSLRIIDLIDREQGEDCLLLLKSLPPAEAEKTAERVIIWARLALQEEPDHSKEGKAWRMSVAKTSVDILKILCDIQKDNLQKKDECEEMLKLFKEVASLQENFEVFLSFEDYSNSSLVADLREQHIKAHEVAQAKHKPGSTPEPIAAEVRSPSMESKLHRQALALQMSKQELEAELTLRALKDGNIKTALKKCSDLFKYHCNADTGKLLFLTCQKLCQMLADNVPVTVPVGLNLPSMIHDLASQAATICSPDFLLDALELCKHTLMAVELSRQCQMDDCGILMKASFGTHKDPYEEWSYSDFFSEDGIVLESQMVLPVIYELISSLVPLAESKRYPLESTSLPYCSLNEGDGLVLPVINSISALLQNLQESSQWELALRFVVGSFGTCLQHSVSNFMNATLSEKLFGETTLVKSRHVVMELKEKAVIFIRENATTLLHKVFNCRLVDLDLALGYCTLLPQKDVFENLWKLIDKAWQNYDKILAISLVGSELASLYQEIEMGLKFRELSTDAQWGIRLGKLGISFQPVFRQHFLTKKDLIKALVENIDMDTSLILEYCSTFQLDCDAVLQLFIETLLHNTNAGQGQGDASMDSAKRRHPKLLAKALEMVPLLTSTKDLVISLSGILHKLDPYDYEMIEVVLKVIERADEKITNININQVI

Target ORF information:

RefSeq Version	XM_011539029.2
Organism	Homo sapiens(human)
Definition	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539029.2

ORF Insert Sequence:

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAACCA 
GTTTTCAGGC AACATTTTCT CACCAAGAAA GACCTCATTA AAGCTCTTGT GGAGAATATA 
GATATGGACA CAAGCCTCAT TTTGGAATAT TGCAGCACAT TTCAGTTGGA CTGCGATGCA 
GTTCTTCAGC TCTTCATTGA AACGCTGCTC CACAACACAA ATGCCGGCCA AGGCCAGGGA 
GATGCAAGCA TGGACTCTGC AAAGCGGCGG CATCCCAAAC TCCTGGCCAA AGCCCTTGAG 
ATGGTTCCTT TACTGACGAG CACAAAAGAT TTGGTCATCA GTCTTAGTGG AATACTACAT 
AAGTTGGATC CTTATGACTA TGAAATGATT GAAGTTGTCT TGAAAGTTAT AGAACGAGCT 
GATGAAAAGA TAACCAATAT TAATATTAAT CAGGTGATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNTC1 ( XM_011539030.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537332.1 Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu63244
Clone ID Related Accession (Same CDS sequence)	XM_011539030.1
Accession Version	XM_011539030.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	kinetochore-associated protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAACCA 
GTTTTCAGGC AACATTTTCT CACCAAGAAA GACCTCATTA AAGCTCTTGT GGAGAATATA 
GATATGGACA CAAGCCTCAT TTTGGAATAT TGCAGATTTC TCTCCAATAT TCTAACAGTT 
TACACACCCA CCAGGAATAA TACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537332.1
CDS	146..4552
Translation	MWNDIELLTNDDTGSGYLSVGSRKEHGTALYQVDLLVKISSEKASLNPKIQACSLSDGFIIVADQSVILLDSICRSLQLHLVFDTEVDVVGLCQEGKFLLVGERSGNLHLIHVTSKQTLLTNAFVQKANDENRRTYQNLVIEKDGSNEGTYYMLLLTYSGFFCITNLQLLKIQQAIENVDFSTAKKLQGQIKSSFISTENYHTLGCLSLVAGDLASEVPVIIGGTGNCAFSKWEPDSSKKGMTVKNLIDAEIIKGAKKFQLIDNLLFVLDTDNVLSLWDIYTLTPVWNWPSLHVEEFLLTTEADSPSSVTWQGITNLKLIALTASANKKMKNLMVYSLPTMEILYSLEVSSVSSLVQTGISTDTIYLLEGVCKNDPKLSEDSVSVLVLRCLTEALPENRLSRLLHKHRFAEAESFAIQFGLDVELVYKVKSNHILEKLALSSVDASEQTEWQQLVDDAKENLHKIQDDEFVVNYCLKAQWITYETTQEMLNYAKTRLLKKEDKTALIYSDGLKEVLRAHAKLTTFYGAFGPEKFSGSSWIEFLNNEDDLKDIFLQLKEGNLVCAQYLWLRHRANFESRFDVKMLESLLNSMSASVSLQKLCPWFKNDVIPFVRRTVPEGQIILAKWLEQAARNLELTDKANWPENGLQLAEIFFTAEKTDELGLASSWHWISLKDYQNTEEVCQLRTLVNNLRELITLHRKYNCKLALSDFEKENTTTIVFRMFDKVLAPELIPSILEKFIRVYMREHDLQEEELLLLYIEDLLNRCSSKSTSLFETAWEAKAMAVIACLSDTDLIFDAVLKIMYAAVVPWSAAVEQLVKQHLEMDHPKVKLLQESYKLMEMKKLLRGYGIREVNLLNKEIMRVVRYILKQDVPSSLEDALKVAQAFMLSDDEIYSLRIIDLIDREQGEDCLLLLKSLPPAEAEKTAERVIIWARLALQEEPDHSKEGKAWRMSVAKTSVDILKILCDIQKDNLQKKDECEEMLKLFKEVASLQENFEVFLSFEDYSNSSLVADLREQHIKAHEVAQAKHKPGSTPEPIAAEVRSPSMESKLHRQALALQMSKQELEAELTLRALKDGNIKTALKKCSDLFKYHCNADTGKLLFLTCQKLCQMLADNVPVTVPVGLNLPSMIHDLASQAATICSPDFLLDALELCKHTLMAVELSRQCQMDDCGILMKASFGTHKDPYEEWSYSDFFSEDGIVLESQMVLPVIYELISSLVPLAESKRYPLESTSLPYCSLNEGDGLVLPVINSISALLQNLQESSQWELALRFVVGSFGTCLQHSVSNFMNATLSEKLFGETTLVKSRHVVMELKEKAVIFIRENATTLLHKVFNCRLVDLDLALGYCTLLPQKDVFENLWKLIDKAWQNYDKILAISLVGSELASLYQEIEMGLKFRELSTDAQWGIRLGKLGISFQPVFRQHFLTKKDLIKALVENIDMDTSLILEYCRFLSNILTVYTPTRNNT

Target ORF information:

RefSeq Version	XM_011539030.1
Organism	Homo sapiens(human)
Definition	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539030.1

ORF Insert Sequence:

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAACCA 
GTTTTCAGGC AACATTTTCT CACCAAGAAA GACCTCATTA AAGCTCTTGT GGAGAATATA 
GATATGGACA CAAGCCTCAT TTTGGAATAT TGCAGATTTC TCTCCAATAT TCTAACAGTT 
TACACACCCA CCAGGAATAA TACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNTC1 ( XM_017020254.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875743.1 Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu84340
Clone ID Related Accession (Same CDS sequence)	XM_017020254.1 , XM_017020254.2
Accession Version	XM_017020254.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	kinetochore-associated protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAACCA 
GTTTTCAGGC AACATTTTCT CACCAAGAAA GACCTCATTA AAGCTCTTGT GGAGAATATA 
GATATGGACA CAAGCCTCAT TTTGGAATAT TGCAGCACAT TTCAGTTGGA CTGCGATGCA 
GTTCTTCAGC TCTTCATTGA AACGCTGCTC CACAACACAA ATGCCGGCCA AGGCCAGGGA 
GATGCAAGCA TGGACTCTGC AAAGCGGCGG CATCCCAAAC TCCTGGCCAA AGCCCTTGAG 
ATGGTTCCTT TACTGACGAG CACAAAAGAT TTGGTCATCA GTCTTAGTGG AATACTACAT 
AAGGCATTGA GTATTCTGAA ACATTTGAAG TCATACAGAA GAATTTCTCC TCCCGTGGAT 
CTAGAATATC AGTATATGTT GGAACATGTC ATAACTTTGC CATCAGCTGC CCAAACTAGA 
CTGCCTTTTC ACCTGATATT CTTTGGCACA GCACAGAACT TCTGGAAAAT TCTCTCTACA 
GAACTCAGTG AAGAATCTTT CCCAACATTG CTCTTAATTT CGAAATTAAT GAAGTTCTCT 
CTGGACACTC TGTACGTGTC TACAGCAAAA CACGTTTTCG AAAAAAAACT GAAGCCAAAG 
CTCCTGAAGT TAACACAAGC TAAATCCTCA ACACTGATTA ACAAGGAAAT AACTAAGATC 
ACGCAGACCA TCGAATCCTG CTTACTCTCT ATAGTCAACC CAGAGTGGGC TGTAGCTATT 
GCCATCAGCC TTGCCCAGGA TATCCCTGAA GGTTCCTTCA AGATATCTGC TTTGAAATTC 
TGCCTTTATT TAGCTGAGAG ATGGCTACAG AATATCCCAT CGCAGGACGA AAAACGTGAA 
AAAGCCGAGG CTTTGTTGAA GAAGCTTCAT ATCCAGTACC GGCGATCGGG CACAGAAGCT 
GTGCTCATAG CCCACAAGCT GAACACTGAG GAATATTTAA GAGTGATCGG AAAGCCAGCA 
CATCTTATTG TCAGTCTCTA CGAACATCCT AGCATCAATC AAAGAATTCA GAATTCATCT 
GGCACAGATT ATCCTGATAT TCATGCAGCA GCTAAAGAAA TAGCCGAAGT CAATGAAATT 
AATTTGGAAA AAGTCTGGGA CATGTTGTTG GAAAAATGGC TATGCCCTTC AACAAAACCT 
GGTGAAAAAC CATCAGAATT ATTTGAACTT CAAGAAGATG AAGCCCTACG AAGAGTGCAG 
TATCTCCTCC TGTCTCGTCC AATTGATTAT AGTTCAAGAA TGCTGTTTGT ATTTGCAACA 
TCAACTACAA CCACATTAGG TATGCATCAG TTAACTTTTG CCCATAGAAC TCGAGCTCTT 
CAGTGTCTCT TCTATTTGGC TGACAAGGAA ACTATAGAAT CTCTCTTTAA AAAACCCATT 
GAAGAAGTGA AATCTTATTT GAGATGTATA ACTTTTCTGG CATCATTTGA GACTTTGAAT 
ATCCCCATCA CATATGAATT ATTTTGCAGC AGTCCTAAAG AAGGAATGAT TAAGGGTCTG 
TGGAAAAACC ACAGCCACGA GTCCATGGCA GTAAGATTGG TGACTGAGCT GTGTTTAGAA 
TACAAAATCT ATGACCTGCA GCTTTGGAAT GGACTCTTGC AAAAGCTTCT GGGCTTCAAT 
ATGATTCCTT ATCTAAGGAA AGTTTTAAAA GCCATCTCCA GTATCCATTC TTTATGGCAG 
GTTCCCTACT TCAGCAAAGC GTGGCAGCGT GTGATACAGA TACCACTGCT TTCAGCCTCT 
TGTCCTTTAA GTCCTGATCA GCTGTCAGAT TGTTCTGAGA GTCTCATCGC TGTCCTCGAA 
TGTCCAGTCT CAGGTGATCT TGACCTGATC GGAGTCGCCA GGCAGTATAT CCAGTTAGAA 
CTTCCGGCTT TTGCATTAGC TTGTCTGATG CTCATGCCCC ACTCAGAGAA AAGACACCAG 
CAAATTAAGA ATTTTCTGGG TTCCTGTGAC CCTCAGGTTA TTTTAAAGCA ATTGGAAGAG 
CATATGAACA CGGGCCAGCT AGCAGGATTT TCACATCAAA TTAGAAGTCT GATTTTGAAT 
AATATCATCA ATAAGAAGGA GTTTGGGATT TTGGCAAAGA CCAAATACTT TCAAATGTTG 
AAGATGCATG CGATGAATAC CAACAATATC ACTGAGCTAG TGAACTATTT GGCAAATGAC 
TTAAGTTTAG ATGAAGCTTC AGTCTTGATA ACTGAATATT CAAAGCACTG CGGGAAACCT 
GTGCCTCCAG ACACTGCTCC CTGTGAAATT CTGAAGATGT TTCTTAGTGG ATTATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875743.1
CDS	146..6685
Translation	MWNDIELLTNDDTGSGYLSVGSRKEHGTALYQVDLLVKISSEKASLNPKIQACSLSDGFIIVADQSVILLDSICRSLQLHLVFDTEVDVVGLCQEGKFLLVGERSGNLHLIHVTSKQTLLTNAFVQKANDENRRTYQNLVIEKDGSNEGTYYMLLLTYSGFFCITNLQLLKIQQAIENVDFSTAKKLQGQIKSSFISTENYHTLGCLSLVAGDLASEVPVIIGGTGNCAFSKWEPDSSKKGMTVKNLIDAEIIKGAKKFQLIDNLLFVLDTDNVLSLWDIYTLTPVWNWPSLHVEEFLLTTEADSPSSVTWQGITNLKLIALTASANKKMKNLMVYSLPTMEILYSLEVSSVSSLVQTGISTDTIYLLEGVCKNDPKLSEDSVSVLVLRCLTEALPENRLSRLLHKHRFAEAESFAIQFGLDVELVYKVKSNHILEKLALSSVDASEQTEWQQLVDDAKENLHKIQDDEFVVNYCLKAQWITYETTQEMLNYAKTRLLKKEDKTALIYSDGLKEVLRAHAKLTTFYGAFGPEKFSGSSWIEFLNNEDDLKDIFLQLKEGNLVCAQYLWLRHRANFESRFDVKMLESLLNSMSASVSLQKLCPWFKNDVIPFVRRTVPEGQIILAKWLEQAARNLELTDKANWPENGLQLAEIFFTAEKTDELGLASSWHWISLKDYQNTEEVCQLRTLVNNLRELITLHRKYNCKLALSDFEKENTTTIVFRMFDKVLAPELIPSILEKFIRVYMREHDLQEEELLLLYIEDLLNRCSSKSTSLFETAWEAKAMAVIACLSDTDLIFDAVLKIMYAAVVPWSAAVEQLVKQHLEMDHPKVKLLQESYKLMEMKKLLRGYGIREVNLLNKEIMRVVRYILKQDVPSSLEDALKVAQAFMLSDDEIYSLRIIDLIDREQGEDCLLLLKSLPPAEAEKTAERVIIWARLALQEEPDHSKEGKAWRMSVAKTSVDILKILCDIQKDNLQKKDECEEMLKLFKEVASLQENFEVFLSFEDYSNSSLVADLREQHIKAHEVAQAKHKPGSTPEPIAAEVRSPSMESKLHRQALALQMSKQELEAELTLRALKDGNIKTALKKCSDLFKYHCNADTGKLLFLTCQKLCQMLADNVPVTVPVGLNLPSMIHDLASQAATICSPDFLLDALELCKHTLMAVELSRQCQMDDCGILMKASFGTHKDPYEEWSYSDFFSEDGIVLESQMVLPVIYELISSLVPLAESKRYPLESTSLPYCSLNEGDGLVLPVINSISALLQNLQESSQWELALRFVVGSFGTCLQHSVSNFMNATLSEKLFGETTLVKSRHVVMELKEKAVIFIRENATTLLHKVFNCRLVDLDLALGYCTLLPQKDVFENLWKLIDKAWQNYDKILAISLVGSELASLYQEIEMGLKFRELSTDAQWGIRLGKLGISFQPVFRQHFLTKKDLIKALVENIDMDTSLILEYCSTFQLDCDAVLQLFIETLLHNTNAGQGQGDASMDSAKRRHPKLLAKALEMVPLLTSTKDLVISLSGILHKALSILKHLKSYRRISPPVDLEYQYMLEHVITLPSAAQTRLPFHLIFFGTAQNFWKILSTELSEESFPTLLLISKLMKFSLDTLYVSTAKHVFEKKLKPKLLKLTQAKSSTLINKEITKITQTIESCLLSIVNPEWAVAIAISLAQDIPEGSFKISALKFCLYLAERWLQNIPSQDEKREKAEALLKKLHIQYRRSGTEAVLIAHKLNTEEYLRVIGKPAHLIVSLYEHPSINQRIQNSSGTDYPDIHAAAKEIAEVNEINLEKVWDMLLEKWLCPSTKPGEKPSELFELQEDEALRRVQYLLLSRPIDYSSRMLFVFATSTTTTLGMHQLTFAHRTRALQCLFYLADKETIESLFKKPIEEVKSYLRCITFLASFETLNIPITYELFCSSPKEGMIKGLWKNHSHESMAVRLVTELCLEYKIYDLQLWNGLLQKLLGFNMIPYLRKVLKAISSIHSLWQVPYFSKAWQRVIQIPLLSASCPLSPDQLSDCSESLIAVLECPVSGDLDLIGVARQYIQLELPAFALACLMLMPHSEKRHQQIKNFLGSCDPQVILKQLEEHMNTGQLAGFSHQIRSLILNNIINKKEFGILAKTKYFQMLKMHAMNTNNITELVNYLANDLSLDEASVLITEYSKHCGKPVPPDTAPCEILKMFLSGLS

Target ORF information:

RefSeq Version	XM_017020254.1
Organism	Homo sapiens(human)
Definition	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020254.1

ORF Insert Sequence:

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAACCA 
GTTTTCAGGC AACATTTTCT CACCAAGAAA GACCTCATTA AAGCTCTTGT GGAGAATATA 
GATATGGACA CAAGCCTCAT TTTGGAATAT TGCAGCACAT TTCAGTTGGA CTGCGATGCA 
GTTCTTCAGC TCTTCATTGA AACGCTGCTC CACAACACAA ATGCCGGCCA AGGCCAGGGA 
GATGCAAGCA TGGACTCTGC AAAGCGGCGG CATCCCAAAC TCCTGGCCAA AGCCCTTGAG 
ATGGTTCCTT TACTGACGAG CACAAAAGAT TTGGTCATCA GTCTTAGTGG AATACTACAT 
AAGGCATTGA GTATTCTGAA ACATTTGAAG TCATACAGAA GAATTTCTCC TCCCGTGGAT 
CTAGAATATC AGTATATGTT GGAACATGTC ATAACTTTGC CATCAGCTGC CCAAACTAGA 
CTGCCTTTTC ACCTGATATT CTTTGGCACA GCACAGAACT TCTGGAAAAT TCTCTCTACA 
GAACTCAGTG AAGAATCTTT CCCAACATTG CTCTTAATTT CGAAATTAAT GAAGTTCTCT 
CTGGACACTC TGTACGTGTC TACAGCAAAA CACGTTTTCG AAAAAAAACT GAAGCCAAAG 
CTCCTGAAGT TAACACAAGC TAAATCCTCA ACACTGATTA ACAAGGAAAT AACTAAGATC 
ACGCAGACCA TCGAATCCTG CTTACTCTCT ATAGTCAACC CAGAGTGGGC TGTAGCTATT 
GCCATCAGCC TTGCCCAGGA TATCCCTGAA GGTTCCTTCA AGATATCTGC TTTGAAATTC 
TGCCTTTATT TAGCTGAGAG ATGGCTACAG AATATCCCAT CGCAGGACGA AAAACGTGAA 
AAAGCCGAGG CTTTGTTGAA GAAGCTTCAT ATCCAGTACC GGCGATCGGG CACAGAAGCT 
GTGCTCATAG CCCACAAGCT GAACACTGAG GAATATTTAA GAGTGATCGG AAAGCCAGCA 
CATCTTATTG TCAGTCTCTA CGAACATCCT AGCATCAATC AAAGAATTCA GAATTCATCT 
GGCACAGATT ATCCTGATAT TCATGCAGCA GCTAAAGAAA TAGCCGAAGT CAATGAAATT 
AATTTGGAAA AAGTCTGGGA CATGTTGTTG GAAAAATGGC TATGCCCTTC AACAAAACCT 
GGTGAAAAAC CATCAGAATT ATTTGAACTT CAAGAAGATG AAGCCCTACG AAGAGTGCAG 
TATCTCCTCC TGTCTCGTCC AATTGATTAT AGTTCAAGAA TGCTGTTTGT ATTTGCAACA 
TCAACTACAA CCACATTAGG TATGCATCAG TTAACTTTTG CCCATAGAAC TCGAGCTCTT 
CAGTGTCTCT TCTATTTGGC TGACAAGGAA ACTATAGAAT CTCTCTTTAA AAAACCCATT 
GAAGAAGTGA AATCTTATTT GAGATGTATA ACTTTTCTGG CATCATTTGA GACTTTGAAT 
ATCCCCATCA CATATGAATT ATTTTGCAGC AGTCCTAAAG AAGGAATGAT TAAGGGTCTG 
TGGAAAAACC ACAGCCACGA GTCCATGGCA GTAAGATTGG TGACTGAGCT GTGTTTAGAA 
TACAAAATCT ATGACCTGCA GCTTTGGAAT GGACTCTTGC AAAAGCTTCT GGGCTTCAAT 
ATGATTCCTT ATCTAAGGAA AGTTTTAAAA GCCATCTCCA GTATCCATTC TTTATGGCAG 
GTTCCCTACT TCAGCAAAGC GTGGCAGCGT GTGATACAGA TACCACTGCT TTCAGCCTCT 
TGTCCTTTAA GTCCTGATCA GCTGTCAGAT TGTTCTGAGA GTCTCATCGC TGTCCTCGAA 
TGTCCAGTCT CAGGTGATCT TGACCTGATC GGAGTCGCCA GGCAGTATAT CCAGTTAGAA 
CTTCCGGCTT TTGCATTAGC TTGTCTGATG CTCATGCCCC ACTCAGAGAA AAGACACCAG 
CAAATTAAGA ATTTTCTGGG TTCCTGTGAC CCTCAGGTTA TTTTAAAGCA ATTGGAAGAG 
CATATGAACA CGGGCCAGCT AGCAGGATTT TCACATCAAA TTAGAAGTCT GATTTTGAAT 
AATATCATCA ATAAGAAGGA GTTTGGGATT TTGGCAAAGA CCAAATACTT TCAAATGTTG 
AAGATGCATG CGATGAATAC CAACAATATC ACTGAGCTAG TGAACTATTT GGCAAATGAC 
TTAAGTTTAG ATGAAGCTTC AGTCTTGATA ACTGAATATT CAAAGCACTG CGGGAAACCT 
GTGCCTCCAG ACACTGCTCC CTGTGAAATT CTGAAGATGT TTCTTAGTGG ATTATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNTC1 ( XM_017020254.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875743.1 Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X3, mRNA.

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CloneID	OHu84340
Clone ID Related Accession (Same CDS sequence)	XM_017020254.1 , XM_017020254.2
Accession Version	XM_017020254.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	kinetochore-associated protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017020254.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAACCA 
GTTTTCAGGC AACATTTTCT CACCAAGAAA GACCTCATTA AAGCTCTTGT GGAGAATATA 
GATATGGACA CAAGCCTCAT TTTGGAATAT TGCAGCACAT TTCAGTTGGA CTGCGATGCA 
GTTCTTCAGC TCTTCATTGA AACGCTGCTC CACAACACAA ATGCCGGCCA AGGCCAGGGA 
GATGCAAGCA TGGACTCTGC AAAGCGGCGG CATCCCAAAC TCCTGGCCAA AGCCCTTGAG 
ATGGTTCCTT TACTGACGAG CACAAAAGAT TTGGTCATCA GTCTTAGTGG AATACTACAT 
AAGGCATTGA GTATTCTGAA ACATTTGAAG TCATACAGAA GAATTTCTCC TCCCGTGGAT 
CTAGAATATC AGTATATGTT GGAACATGTC ATAACTTTGC CATCAGCTGC CCAAACTAGA 
CTGCCTTTTC ACCTGATATT CTTTGGCACA GCACAGAACT TCTGGAAAAT TCTCTCTACA 
GAACTCAGTG AAGAATCTTT CCCAACATTG CTCTTAATTT CGAAATTAAT GAAGTTCTCT 
CTGGACACTC TGTACGTGTC TACAGCAAAA CACGTTTTCG AAAAAAAACT GAAGCCAAAG 
CTCCTGAAGT TAACACAAGC TAAATCCTCA ACACTGATTA ACAAGGAAAT AACTAAGATC 
ACGCAGACCA TCGAATCCTG CTTACTCTCT ATAGTCAACC CAGAGTGGGC TGTAGCTATT 
GCCATCAGCC TTGCCCAGGA TATCCCTGAA GGTTCCTTCA AGATATCTGC TTTGAAATTC 
TGCCTTTATT TAGCTGAGAG ATGGCTACAG AATATCCCAT CGCAGGACGA AAAACGTGAA 
AAAGCCGAGG CTTTGTTGAA GAAGCTTCAT ATCCAGTACC GGCGATCGGG CACAGAAGCT 
GTGCTCATAG CCCACAAGCT GAACACTGAG GAATATTTAA GAGTGATCGG AAAGCCAGCA 
CATCTTATTG TCAGTCTCTA CGAACATCCT AGCATCAATC AAAGAATTCA GAATTCATCT 
GGCACAGATT ATCCTGATAT TCATGCAGCA GCTAAAGAAA TAGCCGAAGT CAATGAAATT 
AATTTGGAAA AAGTCTGGGA CATGTTGTTG GAAAAATGGC TATGCCCTTC AACAAAACCT 
GGTGAAAAAC CATCAGAATT ATTTGAACTT CAAGAAGATG AAGCCCTACG AAGAGTGCAG 
TATCTCCTCC TGTCTCGTCC AATTGATTAT AGTTCAAGAA TGCTGTTTGT ATTTGCAACA 
TCAACTACAA CCACATTAGG TATGCATCAG TTAACTTTTG CCCATAGAAC TCGAGCTCTT 
CAGTGTCTCT TCTATTTGGC TGACAAGGAA ACTATAGAAT CTCTCTTTAA AAAACCCATT 
GAAGAAGTGA AATCTTATTT GAGATGTATA ACTTTTCTGG CATCATTTGA GACTTTGAAT 
ATCCCCATCA CATATGAATT ATTTTGCAGC AGTCCTAAAG AAGGAATGAT TAAGGGTCTG 
TGGAAAAACC ACAGCCACGA GTCCATGGCA GTAAGATTGG TGACTGAGCT GTGTTTAGAA 
TACAAAATCT ATGACCTGCA GCTTTGGAAT GGACTCTTGC AAAAGCTTCT GGGCTTCAAT 
ATGATTCCTT ATCTAAGGAA AGTTTTAAAA GCCATCTCCA GTATCCATTC TTTATGGCAG 
GTTCCCTACT TCAGCAAAGC GTGGCAGCGT GTGATACAGA TACCACTGCT TTCAGCCTCT 
TGTCCTTTAA GTCCTGATCA GCTGTCAGAT TGTTCTGAGA GTCTCATCGC TGTCCTCGAA 
TGTCCAGTCT CAGGTGATCT TGACCTGATC GGAGTCGCCA GGCAGTATAT CCAGTTAGAA 
CTTCCGGCTT TTGCATTAGC TTGTCTGATG CTCATGCCCC ACTCAGAGAA AAGACACCAG 
CAAATTAAGA ATTTTCTGGG TTCCTGTGAC CCTCAGGTTA TTTTAAAGCA ATTGGAAGAG 
CATATGAACA CGGGCCAGCT AGCAGGATTT TCACATCAAA TTAGAAGTCT GATTTTGAAT 
AATATCATCA ATAAGAAGGA GTTTGGGATT TTGGCAAAGA CCAAATACTT TCAAATGTTG 
AAGATGCATG CGATGAATAC CAACAATATC ACTGAGCTAG TGAACTATTT GGCAAATGAC 
TTAAGTTTAG ATGAAGCTTC AGTCTTGATA ACTGAATATT CAAAGCACTG CGGGAAACCT 
GTGCCTCCAG ACACTGCTCC CTGTGAAATT CTGAAGATGT TTCTTAGTGG ATTATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875743.1
CDS	146..6685
Translation	MWNDIELLTNDDTGSGYLSVGSRKEHGTALYQVDLLVKISSEKASLNPKIQACSLSDGFIIVADQSVILLDSICRSLQLHLVFDTEVDVVGLCQEGKFLLVGERSGNLHLIHVTSKQTLLTNAFVQKANDENRRTYQNLVIEKDGSNEGTYYMLLLTYSGFFCITNLQLLKIQQAIENVDFSTAKKLQGQIKSSFISTENYHTLGCLSLVAGDLASEVPVIIGGTGNCAFSKWEPDSSKKGMTVKNLIDAEIIKGAKKFQLIDNLLFVLDTDNVLSLWDIYTLTPVWNWPSLHVEEFLLTTEADSPSSVTWQGITNLKLIALTASANKKMKNLMVYSLPTMEILYSLEVSSVSSLVQTGISTDTIYLLEGVCKNDPKLSEDSVSVLVLRCLTEALPENRLSRLLHKHRFAEAESFAIQFGLDVELVYKVKSNHILEKLALSSVDASEQTEWQQLVDDAKENLHKIQDDEFVVNYCLKAQWITYETTQEMLNYAKTRLLKKEDKTALIYSDGLKEVLRAHAKLTTFYGAFGPEKFSGSSWIEFLNNEDDLKDIFLQLKEGNLVCAQYLWLRHRANFESRFDVKMLESLLNSMSASVSLQKLCPWFKNDVIPFVRRTVPEGQIILAKWLEQAARNLELTDKANWPENGLQLAEIFFTAEKTDELGLASSWHWISLKDYQNTEEVCQLRTLVNNLRELITLHRKYNCKLALSDFEKENTTTIVFRMFDKVLAPELIPSILEKFIRVYMREHDLQEEELLLLYIEDLLNRCSSKSTSLFETAWEAKAMAVIACLSDTDLIFDAVLKIMYAAVVPWSAAVEQLVKQHLEMDHPKVKLLQESYKLMEMKKLLRGYGIREVNLLNKEIMRVVRYILKQDVPSSLEDALKVAQAFMLSDDEIYSLRIIDLIDREQGEDCLLLLKSLPPAEAEKTAERVIIWARLALQEEPDHSKEGKAWRMSVAKTSVDILKILCDIQKDNLQKKDECEEMLKLFKEVASLQENFEVFLSFEDYSNSSLVADLREQHIKAHEVAQAKHKPGSTPEPIAAEVRSPSMESKLHRQALALQMSKQELEAELTLRALKDGNIKTALKKCSDLFKYHCNADTGKLLFLTCQKLCQMLADNVPVTVPVGLNLPSMIHDLASQAATICSPDFLLDALELCKHTLMAVELSRQCQMDDCGILMKASFGTHKDPYEEWSYSDFFSEDGIVLESQMVLPVIYELISSLVPLAESKRYPLESTSLPYCSLNEGDGLVLPVINSISALLQNLQESSQWELALRFVVGSFGTCLQHSVSNFMNATLSEKLFGETTLVKSRHVVMELKEKAVIFIRENATTLLHKVFNCRLVDLDLALGYCTLLPQKDVFENLWKLIDKAWQNYDKILAISLVGSELASLYQEIEMGLKFRELSTDAQWGIRLGKLGISFQPVFRQHFLTKKDLIKALVENIDMDTSLILEYCSTFQLDCDAVLQLFIETLLHNTNAGQGQGDASMDSAKRRHPKLLAKALEMVPLLTSTKDLVISLSGILHKALSILKHLKSYRRISPPVDLEYQYMLEHVITLPSAAQTRLPFHLIFFGTAQNFWKILSTELSEESFPTLLLISKLMKFSLDTLYVSTAKHVFEKKLKPKLLKLTQAKSSTLINKEITKITQTIESCLLSIVNPEWAVAIAISLAQDIPEGSFKISALKFCLYLAERWLQNIPSQDEKREKAEALLKKLHIQYRRSGTEAVLIAHKLNTEEYLRVIGKPAHLIVSLYEHPSINQRIQNSSGTDYPDIHAAAKEIAEVNEINLEKVWDMLLEKWLCPSTKPGEKPSELFELQEDEALRRVQYLLLSRPIDYSSRMLFVFATSTTTTLGMHQLTFAHRTRALQCLFYLADKETIESLFKKPIEEVKSYLRCITFLASFETLNIPITYELFCSSPKEGMIKGLWKNHSHESMAVRLVTELCLEYKIYDLQLWNGLLQKLLGFNMIPYLRKVLKAISSIHSLWQVPYFSKAWQRVIQIPLLSASCPLSPDQLSDCSESLIAVLECPVSGDLDLIGVARQYIQLELPAFALACLMLMPHSEKRHQQIKNFLGSCDPQVILKQLEEHMNTGQLAGFSHQIRSLILNNIINKKEFGILAKTKYFQMLKMHAMNTNNITELVNYLANDLSLDEASVLITEYSKHCGKPVPPDTAPCEILKMFLSGLS

Target ORF information:

RefSeq Version	XM_017020254.2
Organism	Homo sapiens(human)
Definition	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020254.2

ORF Insert Sequence:

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAACCA 
GTTTTCAGGC AACATTTTCT CACCAAGAAA GACCTCATTA AAGCTCTTGT GGAGAATATA 
GATATGGACA CAAGCCTCAT TTTGGAATAT TGCAGCACAT TTCAGTTGGA CTGCGATGCA 
GTTCTTCAGC TCTTCATTGA AACGCTGCTC CACAACACAA ATGCCGGCCA AGGCCAGGGA 
GATGCAAGCA TGGACTCTGC AAAGCGGCGG CATCCCAAAC TCCTGGCCAA AGCCCTTGAG 
ATGGTTCCTT TACTGACGAG CACAAAAGAT TTGGTCATCA GTCTTAGTGG AATACTACAT 
AAGGCATTGA GTATTCTGAA ACATTTGAAG TCATACAGAA GAATTTCTCC TCCCGTGGAT 
CTAGAATATC AGTATATGTT GGAACATGTC ATAACTTTGC CATCAGCTGC CCAAACTAGA 
CTGCCTTTTC ACCTGATATT CTTTGGCACA GCACAGAACT TCTGGAAAAT TCTCTCTACA 
GAACTCAGTG AAGAATCTTT CCCAACATTG CTCTTAATTT CGAAATTAAT GAAGTTCTCT 
CTGGACACTC TGTACGTGTC TACAGCAAAA CACGTTTTCG AAAAAAAACT GAAGCCAAAG 
CTCCTGAAGT TAACACAAGC TAAATCCTCA ACACTGATTA ACAAGGAAAT AACTAAGATC 
ACGCAGACCA TCGAATCCTG CTTACTCTCT ATAGTCAACC CAGAGTGGGC TGTAGCTATT 
GCCATCAGCC TTGCCCAGGA TATCCCTGAA GGTTCCTTCA AGATATCTGC TTTGAAATTC 
TGCCTTTATT TAGCTGAGAG ATGGCTACAG AATATCCCAT CGCAGGACGA AAAACGTGAA 
AAAGCCGAGG CTTTGTTGAA GAAGCTTCAT ATCCAGTACC GGCGATCGGG CACAGAAGCT 
GTGCTCATAG CCCACAAGCT GAACACTGAG GAATATTTAA GAGTGATCGG AAAGCCAGCA 
CATCTTATTG TCAGTCTCTA CGAACATCCT AGCATCAATC AAAGAATTCA GAATTCATCT 
GGCACAGATT ATCCTGATAT TCATGCAGCA GCTAAAGAAA TAGCCGAAGT CAATGAAATT 
AATTTGGAAA AAGTCTGGGA CATGTTGTTG GAAAAATGGC TATGCCCTTC AACAAAACCT 
GGTGAAAAAC CATCAGAATT ATTTGAACTT CAAGAAGATG AAGCCCTACG AAGAGTGCAG 
TATCTCCTCC TGTCTCGTCC AATTGATTAT AGTTCAAGAA TGCTGTTTGT ATTTGCAACA 
TCAACTACAA CCACATTAGG TATGCATCAG TTAACTTTTG CCCATAGAAC TCGAGCTCTT 
CAGTGTCTCT TCTATTTGGC TGACAAGGAA ACTATAGAAT CTCTCTTTAA AAAACCCATT 
GAAGAAGTGA AATCTTATTT GAGATGTATA ACTTTTCTGG CATCATTTGA GACTTTGAAT 
ATCCCCATCA CATATGAATT ATTTTGCAGC AGTCCTAAAG AAGGAATGAT TAAGGGTCTG 
TGGAAAAACC ACAGCCACGA GTCCATGGCA GTAAGATTGG TGACTGAGCT GTGTTTAGAA 
TACAAAATCT ATGACCTGCA GCTTTGGAAT GGACTCTTGC AAAAGCTTCT GGGCTTCAAT 
ATGATTCCTT ATCTAAGGAA AGTTTTAAAA GCCATCTCCA GTATCCATTC TTTATGGCAG 
GTTCCCTACT TCAGCAAAGC GTGGCAGCGT GTGATACAGA TACCACTGCT TTCAGCCTCT 
TGTCCTTTAA GTCCTGATCA GCTGTCAGAT TGTTCTGAGA GTCTCATCGC TGTCCTCGAA 
TGTCCAGTCT CAGGTGATCT TGACCTGATC GGAGTCGCCA GGCAGTATAT CCAGTTAGAA 
CTTCCGGCTT TTGCATTAGC TTGTCTGATG CTCATGCCCC ACTCAGAGAA AAGACACCAG 
CAAATTAAGA ATTTTCTGGG TTCCTGTGAC CCTCAGGTTA TTTTAAAGCA ATTGGAAGAG 
CATATGAACA CGGGCCAGCT AGCAGGATTT TCACATCAAA TTAGAAGTCT GATTTTGAAT 
AATATCATCA ATAAGAAGGA GTTTGGGATT TTGGCAAAGA CCAAATACTT TCAAATGTTG 
AAGATGCATG CGATGAATAC CAACAATATC ACTGAGCTAG TGAACTATTT GGCAAATGAC 
TTAAGTTTAG ATGAAGCTTC AGTCTTGATA ACTGAATATT CAAAGCACTG CGGGAAACCT 
GTGCCTCCAG ACACTGCTCC CTGTGAAATT CTGAAGATGT TTCTTAGTGG ATTATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNTC1 ( XM_017020255.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875744.1 Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X4, mRNA.

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CloneID	OHu84341
Clone ID Related Accession (Same CDS sequence)	XM_017020255.2 , XM_017020255.1
Accession Version	XM_017020255.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	kinetochore-associated protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017020255.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACTTC TTACATACAG TGGATTTTTT TGTATTACAA ACCTTCAGCT TTTAAAAATT 
CAACAAGCAA TTGAGAATGT AGACTTCAGT ACAGCAAAAA AGTTACAAGG ACAAATCAAG 
TCCAGTTTTA TTTCTACTGA AAATTATCAT ACTCTTGGTT GTCTCAGTCT TGTGGCTGGA 
GATTTAGCAA GTGAAGTTCC TGTGATAATT GGGGGAACCG GTAATTGTGC ATTCTCAAAA 
TGGGAACCAG ATTCTTCCAA GAAAGGAATG ACAGTTAAGA ACCTTATTGA TGCAGAGATT 
ATTAAAGGTG CAAAGAAGTT CCAGCTGATA GACAATCTAC TTTTTGTTCT TGATACTGAT 
AACGTGCTGA GTTTATGGGA TATTTACACT CTAACTCCTG TATGGAACTG GCCCTCTCTT 
CACGTAGAAG AGTTTCTTCT TACTACAGAA GCAGACTCTC CTTCATCAGT CACGTGGCAA 
GGAATTACAA ATCTCAAATT AATAGCTCTG ACAGCTTCAG CTAATAAGAA GATGAAAAAC 
CTCATGGTTT ATTCATTACC TACAATGGAA ATACTATATT CTTTGGAAGT ATCTAGTGTT 
TCTTCTCTGG TCCAAACAGG AATTAGCACA GATACCATAT ACCTTTTAGA AGGAGTTTGC 
AAAAATGATC CAAAATTGTC TGAAGACTCA GTCTCTGTGT TAGTACTCAG ATGTCTTACG 
GAAGCTTTAC CAGAAAACAG ATTGAGTCGG TTACTTCACA AACACAGATT TGCTGAAGCT 
GAGAGTTTTG CCATTCAGTT TGGACTAGAT GTTGAGCTTG TTTACAAGGT CAAGTCAAAT 
CATATATTGG AGAAACTGGC ATTGAGTTCT GTGGATGCCA GTGAACAGAC CGAATGGCAA 
CAACTTGTAG ACGACGCTAA GGAAAATCTA CATAAGATCC AGGATGATGA ATTTGTGGTG 
AATTACTGCC TGAAAGCTCA GTGGATAACC TATGAAACCA CTCAAGAGAT GCTGAATTAT 
GCCAAAACCA GGCTTTTGAA GAAAGAAGAT AAAACTGCTC TCATTTATTC TGATGGCTTG 
AAAGAGGTGC TAAGAGCTCA TGCAAAATTG ACTACTTTTT ATGGAGCATT TGGACCAGAA 
AAATTCAGTG GCAGTTCTTG GATTGAATTT CTAAATAATG AAGATGATCT TAAAGATATT 
TTTTTACAGC TAAAAGAAGG AAACCTTGTT TGTGCACAGT ATCTTTGGCT TCGACATCGG 
GCAAACTTTG AAAGCAGATT TGATGTGAAA ATGCTGGAGA GCTTGCTCAA CTCAATGTCT 
GCATCAGTCT CTTTGCAAAA GCTGTGTCCA TGGTTTAAAA ATGATGTGAT TCCATTTGTA 
AGAAGGACTG TGCCTGAAGG ACAGATAATT CTTGCAAAAT GGTTGGAACA AGCAGCCAGG 
AACCTTGAAT TAACTGATAA GGCAAATTGG CCAGAAAATG GACTTCAATT GGCAGAGATA 
TTTTTTACAG CAGAAAAAAC AGACGAGTTG GGATTGGCAT CTTCCTGGCA TTGGATTTCC 
TTGAAAGATT ATCAGAACAC AGAGGAAGTA TGTCAGCTAA GGACTTTGGT AAATAACTTG 
CGAGAGTTGA TCACGTTGCA TAGGAAGTAC AACTGCAAAT TAGCCCTCTC TGATTTTGAG 
AAGGAAAATA CAACCACCAT AGTGTTCCGA ATGTTTGATA AAGTGCTGGC CCCAGAGCTT 
ATTCCCTCCA TCTTAGAGAA GTTTATAAGA GTTTACATGA GAGAACATGA CTTGCAAGAG 
GAGGAACTTC TCTTGCTGTA CATAGAGGAT TTACTGAATA GATGCAGCTC AAAGTCCACA 
TCACTCTTTG AAACAGCATG GGAAGCAAAG GCCATGGCAG TAATAGCGTG TTTATCTGAC 
ACGGACCTCA TATTTGATGC CGTGCTCAAG ATCATGTATG CGGCAGTGGT TCCTTGGAGT 
GCAGCTGTGG AGCAACTGGT GAAACAGCAC CTGGAAATGG ACCATCCCAA AGTCAAGTTA 
TTACAGGAAA GTTACAAACT AATGGAGATG AAAAAACTTT TACGAGGCTA TGGAATAAGA 
GAGGTAAATC TCTTAAACAA GGAAATAATG AGAGTGGTTA GATACATTCT CAAACAAGAT 
GTCCCATCTT CTTTAGAAGA TGCTTTAAAG GTAGCCCAAG CGTTTATGTT ATCTGATGAT 
GAGATCTACA GTCTAAGAAT TATTGACCTG ATTGATAGAG AACAGGGTGA AGACTGTCTC 
CTTCTGTTGA AGTCTTTGCC TCCTGCTGAA GCTGAGAAAA CTGCAGAAAG AGTCATCATA 
TGGGCACGAC TGGCATTACA AGAAGAGCCA GATCATTCTA AAGAGGGCAA GGCCTGGAGA 
ATGTCTGTAG CGAAGACATC CGTGGACATT CTTAAGATAC TATGTGACAT TCAGAAAGAC 
AATCTGCAGA AGAAGGACGA ATGTGAAGAA ATGTTGAAAC TATTTAAAGA GGTTGCTAGC 
TTACAGGAGA ACTTTGAGGT CTTTCTTTCA TTTGAAGATT ATAGCAATAG TTCCCTGGTA 
GCAGATCTCC GTGAGCAGCA CATTAAAGCT CACGAAGTTG CACAGGCGAA ACACAAACCT 
GGGAGCACCC CAGAGCCCAT AGCTGCTGAG GTGAGGAGCC CAAGCATGGA ATCAAAGCTG 
CACAGACAGG CACTGGCCCT GCAGATGTCC AAACAAGAGC TGGAGGCAGA GCTGACCTTG 
AGAGCCTTAA AAGATGGGAA CATCAAAACA GCACTGAAAA AATGCAGCGA CTTGTTTAAG 
TATCACTGCA ATGCTGACAC TGGGAAATTG CTATTTCTGA CATGTCAGAA GCTTTGTCAG 
ATGTTGGCTG ATAATGTCCC AGTGACAGTG CCTGTGGGAC TGAATCTTCC TTCCATGATA 
CATGATCTAG CAAGCCAAGC TGCCACCATT TGCAGTCCAG ATTTTTTACT AGATGCTTTA 
GAACTATGTA AACATACTTT AATGGCTGTA GAGCTTTCCA GACAATGCCA AATGGATGAC 
TGTGGAATCC TCATGAAAGC TTCTTTTGGG ACACATAAAG ATCCATATGA AGAGTGGTCT 
TACAGTGACT TCTTCAGTGA AGATGGAATT GTTCTTGAGT CACAGATGGT GCTTCCAGTG 
ATTTATGAAC TGATTTCATC TCTTGTGCCT CTAGCTGAAA GCAAGAGATA TCCCTTGGAG 
TCTACCAGTT TGCCATACTG CTCCCTTAAT GAAGGAGATG GCCTTGTTTT ACCTGTTATA 
AATTCCATCT CTGCCCTGCT TCAGAATCTT CAGGAATCTA GCCAGTGGGA GCTAGCCCTA 
AGATTTGTGG TTGGTTCATT TGGTACCTGT CTTCAGCACT CTGTGTCAAA CTTCATGAAT 
GCCACTTTGA GTGAAAAGTT ATTTGGAGAG ACTACATTAG TTAAATCAAG GCATGTTGTT 
ATGGAATTGA AAGAAAAAGC TGTTATATTT ATCAGGGAAA ATGCTACAAC ACTACTGCAC 
AAAGTATTTA ATTGTCGCTT GGTAGATCTT GACCTGGCGT TGGGTTACTG CACTCTCTTA 
CCTCAAAAAG ATGTGTTTGA AAATCTCTGG AAGCTCATAG ATAAAGCATG GCAGAATTAC 
GACAAAATCT TGGCAATATC TCTGGTGGGC TCTGAGCTGG CAAGTCTCTA TCAGGAAATA 
GAAATGGGGC TTAAGTTCCG TGAACTCAGT ACTGATGCCC AGTGGGGCAT TCGTCTTGGT 
AAACTTGGTA TTTCTTTTCA ACCAGTTTTC AGGCAACATT TTCTCACCAA GAAAGACCTC 
ATTAAAGCTC TTGTGGAGAA TATAGATATG GACACAAGCC TCATTTTGGA ATATTGCAGC 
ACATTTCAGT TGGACTGCGA TGCAGTTCTT CAGCTCTTCA TTGAAACGCT GCTCCACAAC 
ACAAATGCCG GCCAAGGCCA GGGAGATGCA AGCATGGACT CTGCAAAGCG GCGGCATCCC 
AAACTCCTGG CCAAAGCCCT TGAGATGGTT CCTTTACTGA CGAGCACAAA AGATTTGGTC 
ATCAGTCTTA GTGGAATACT ACATAAGTTG GATCCTTATG ACTATGAAAT GATTGAAGTT 
GTCTTGAAAG TTATAGAACG AGCTGATGAA AAGATAACCA ATATTAATAT TAATCAGGCA 
TTGAGTATTC TGAAACATTT GAAGTCATAC AGAAGAATTT CTCCTCCCGT GGATCTAGAA 
TATCAGTATA TGTTGGAACA TGTCATAACT TTGCCATCAG CTGCCCAAAC TAGACTGCCT 
TTTCACCTGA TATTCTTTGG CACAGCACAG AACTTCTGGA AAATTCTCTC TACAGAACTC 
AGTGAAGAAT CTTTCCCAAC ATTGCTCTTA ATTTCGAAAT TAATGAAGTT CTCTCTGGAC 
ACTCTGTACG TGTCTACAGC AAAACACGTT TTCGAAAAAA AACTGAAGCC AAAGCTCCTG 
AAGTTAACAC AAGCTAAATC CTCAACACTG ATTAACAAGG AAATAACTAA GATCACGCAG 
ACCATCGAAT CCTGCTTACT CTCTATAGTC AACCCAGAGT GGGCTGTAGC TATTGCCATC 
AGCCTTGCCC AGGATATCCC TGAAGGTTCC TTCAAGATAT CTGCTTTGAA ATTCTGCCTT 
TATTTAGCTG AGAGATGGCT ACAGAATATC CCATCGCAGG ACGAAAAACG TGAAAAAGCC 
GAGGCTTTGT TGAAGAAGCT TCATATCCAG TACCGGCGAT CGGGCACAGA AGCTGTGCTC 
ATAGCCCACA AGCTGAACAC TGAGGAATAT TTAAGAGTGA TCGGAAAGCC AGCACATCTT 
ATTGTCAGTC TCTACGAACA TCCTAGCATC AATCAAAGAA TTCAGAATTC ATCTGGCACA 
GATTATCCTG ATATTCATGC AGCAGCTAAA GAAATAGCCG AAGTCAATGA AATTAATTTG 
GAAAAAGTCT GGGACATGTT GTTGGAAAAA TGGCTATGCC CTTCAACAAA ACCTGGTGAA 
AAACCATCAG AATTATTTGA ACTTCAAGAA GATGAAGCCC TACGAAGAGT GCAGTATCTC 
CTCCTGTCTC GTCCAATTGA TTATAGTTCA AGAATGCTGT TTGTATTTGC AACATCAACT 
ACAACCACAT TAGGTATGCA TCAGTTAACT TTTGCCCATA GAACTCGAGC TCTTCAGTGT 
CTCTTCTATT TGGCTGACAA GGAAACTATA GAATCTCTCT TTAAAAAACC CATTGAAGAA 
GTGAAATCTT ATTTGAGATG TATAACTTTT CTGGCATCAT TTGAGACTTT GAATATCCCC 
ATCACATATG AATTATTTTG CAGCAGTCCT AAAGAAGGAA TGATTAAGGG TCTGTGGAAA 
AACCACAGCC ACGAGTCCAT GGCAGTAAGA TTGGTGACTG AGCTGTGTTT AGAATACAAA 
ATCTATGACC TGCAGCTTTG GAATGGACTC TTGCAAAAGC TTCTGGGCTT CAATATGATT 
CCTTATCTAA GGAAAGTTTT AAAAGCCATC TCCAGTATCC ATTCTTTATG GCAGGTTCCC 
TACTTCAGCA AAGCGTGGCA GCGTGTGATA CAGATACCAC TGCTTTCAGC CTCTTGTCCT 
TTAAGTCCTG ATCAGCTGTC AGATTGTTCT GAGAGTCTCA TCGCTGTCCT CGAATGTCCA 
GTCTCAGGTG ATCTTGACCT GATCGGAGTC GCCAGGCAGT ATATCCAGTT AGAACTTCCG 
GCTTTTGCAT TAGCTTGTCT GATGCTCATG CCCCACTCAG AGAAAAGACA CCAGCAAATT 
AAGAATTTTC TGGGTTCCTG TGACCCTCAG GTTATTTTAA AGCAATTGGA AGAGCATATG 
AACACGGGCC AGCTAGCAGG ATTTTCACAT CAAATTAGAA GTCTGATTTT GAATAATATC 
ATCAATAAGA AGGAGTTTGG GATTTTGGCA AAGACCAAAT ACTTTCAAAT GTTGAAGATG 
CATGCGATGA ATACCAACAA TATCACTGAG CTAGTGAACT ATTTGGCAAA TGACTTAAGT 
TTAGATGAAG CTTCAGTCTT GATAACTGAA TATTCAAAGC ACTGCGGGAA ACCTGTGCCT 
CCAGACACTG CTCCCTGTGA AATTCTGAAG ATGTTTCTTA GTGGATTATC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875744.1
CDS	574..6747
Translation	MLLLTYSGFFCITNLQLLKIQQAIENVDFSTAKKLQGQIKSSFISTENYHTLGCLSLVAGDLASEVPVIIGGTGNCAFSKWEPDSSKKGMTVKNLIDAEIIKGAKKFQLIDNLLFVLDTDNVLSLWDIYTLTPVWNWPSLHVEEFLLTTEADSPSSVTWQGITNLKLIALTASANKKMKNLMVYSLPTMEILYSLEVSSVSSLVQTGISTDTIYLLEGVCKNDPKLSEDSVSVLVLRCLTEALPENRLSRLLHKHRFAEAESFAIQFGLDVELVYKVKSNHILEKLALSSVDASEQTEWQQLVDDAKENLHKIQDDEFVVNYCLKAQWITYETTQEMLNYAKTRLLKKEDKTALIYSDGLKEVLRAHAKLTTFYGAFGPEKFSGSSWIEFLNNEDDLKDIFLQLKEGNLVCAQYLWLRHRANFESRFDVKMLESLLNSMSASVSLQKLCPWFKNDVIPFVRRTVPEGQIILAKWLEQAARNLELTDKANWPENGLQLAEIFFTAEKTDELGLASSWHWISLKDYQNTEEVCQLRTLVNNLRELITLHRKYNCKLALSDFEKENTTTIVFRMFDKVLAPELIPSILEKFIRVYMREHDLQEEELLLLYIEDLLNRCSSKSTSLFETAWEAKAMAVIACLSDTDLIFDAVLKIMYAAVVPWSAAVEQLVKQHLEMDHPKVKLLQESYKLMEMKKLLRGYGIREVNLLNKEIMRVVRYILKQDVPSSLEDALKVAQAFMLSDDEIYSLRIIDLIDREQGEDCLLLLKSLPPAEAEKTAERVIIWARLALQEEPDHSKEGKAWRMSVAKTSVDILKILCDIQKDNLQKKDECEEMLKLFKEVASLQENFEVFLSFEDYSNSSLVADLREQHIKAHEVAQAKHKPGSTPEPIAAEVRSPSMESKLHRQALALQMSKQELEAELTLRALKDGNIKTALKKCSDLFKYHCNADTGKLLFLTCQKLCQMLADNVPVTVPVGLNLPSMIHDLASQAATICSPDFLLDALELCKHTLMAVELSRQCQMDDCGILMKASFGTHKDPYEEWSYSDFFSEDGIVLESQMVLPVIYELISSLVPLAESKRYPLESTSLPYCSLNEGDGLVLPVINSISALLQNLQESSQWELALRFVVGSFGTCLQHSVSNFMNATLSEKLFGETTLVKSRHVVMELKEKAVIFIRENATTLLHKVFNCRLVDLDLALGYCTLLPQKDVFENLWKLIDKAWQNYDKILAISLVGSELASLYQEIEMGLKFRELSTDAQWGIRLGKLGISFQPVFRQHFLTKKDLIKALVENIDMDTSLILEYCSTFQLDCDAVLQLFIETLLHNTNAGQGQGDASMDSAKRRHPKLLAKALEMVPLLTSTKDLVISLSGILHKLDPYDYEMIEVVLKVIERADEKITNININQALSILKHLKSYRRISPPVDLEYQYMLEHVITLPSAAQTRLPFHLIFFGTAQNFWKILSTELSEESFPTLLLISKLMKFSLDTLYVSTAKHVFEKKLKPKLLKLTQAKSSTLINKEITKITQTIESCLLSIVNPEWAVAIAISLAQDIPEGSFKISALKFCLYLAERWLQNIPSQDEKREKAEALLKKLHIQYRRSGTEAVLIAHKLNTEEYLRVIGKPAHLIVSLYEHPSINQRIQNSSGTDYPDIHAAAKEIAEVNEINLEKVWDMLLEKWLCPSTKPGEKPSELFELQEDEALRRVQYLLLSRPIDYSSRMLFVFATSTTTTLGMHQLTFAHRTRALQCLFYLADKETIESLFKKPIEEVKSYLRCITFLASFETLNIPITYELFCSSPKEGMIKGLWKNHSHESMAVRLVTELCLEYKIYDLQLWNGLLQKLLGFNMIPYLRKVLKAISSIHSLWQVPYFSKAWQRVIQIPLLSASCPLSPDQLSDCSESLIAVLECPVSGDLDLIGVARQYIQLELPAFALACLMLMPHSEKRHQQIKNFLGSCDPQVILKQLEEHMNTGQLAGFSHQIRSLILNNIINKKEFGILAKTKYFQMLKMHAMNTNNITELVNYLANDLSLDEASVLITEYSKHCGKPVPPDTAPCEILKMFLSGLS

Target ORF information:

RefSeq Version	XM_017020255.2
Organism	Homo sapiens(human)
Definition	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020255.2

ORF Insert Sequence:

ATGCTACTTC TTACATACAG TGGATTTTTT TGTATTACAA ACCTTCAGCT TTTAAAAATT 
CAACAAGCAA TTGAGAATGT AGACTTCAGT ACAGCAAAAA AGTTACAAGG ACAAATCAAG 
TCCAGTTTTA TTTCTACTGA AAATTATCAT ACTCTTGGTT GTCTCAGTCT TGTGGCTGGA 
GATTTAGCAA GTGAAGTTCC TGTGATAATT GGGGGAACCG GTAATTGTGC ATTCTCAAAA 
TGGGAACCAG ATTCTTCCAA GAAAGGAATG ACAGTTAAGA ACCTTATTGA TGCAGAGATT 
ATTAAAGGTG CAAAGAAGTT CCAGCTGATA GACAATCTAC TTTTTGTTCT TGATACTGAT 
AACGTGCTGA GTTTATGGGA TATTTACACT CTAACTCCTG TATGGAACTG GCCCTCTCTT 
CACGTAGAAG AGTTTCTTCT TACTACAGAA GCAGACTCTC CTTCATCAGT CACGTGGCAA 
GGAATTACAA ATCTCAAATT AATAGCTCTG ACAGCTTCAG CTAATAAGAA GATGAAAAAC 
CTCATGGTTT ATTCATTACC TACAATGGAA ATACTATATT CTTTGGAAGT ATCTAGTGTT 
TCTTCTCTGG TCCAAACAGG AATTAGCACA GATACCATAT ACCTTTTAGA AGGAGTTTGC 
AAAAATGATC CAAAATTGTC TGAAGACTCA GTCTCTGTGT TAGTACTCAG ATGTCTTACG 
GAAGCTTTAC CAGAAAACAG ATTGAGTCGG TTACTTCACA AACACAGATT TGCTGAAGCT 
GAGAGTTTTG CCATTCAGTT TGGACTAGAT GTTGAGCTTG TTTACAAGGT CAAGTCAAAT 
CATATATTGG AGAAACTGGC ATTGAGTTCT GTGGATGCCA GTGAACAGAC CGAATGGCAA 
CAACTTGTAG ACGACGCTAA GGAAAATCTA CATAAGATCC AGGATGATGA ATTTGTGGTG 
AATTACTGCC TGAAAGCTCA GTGGATAACC TATGAAACCA CTCAAGAGAT GCTGAATTAT 
GCCAAAACCA GGCTTTTGAA GAAAGAAGAT AAAACTGCTC TCATTTATTC TGATGGCTTG 
AAAGAGGTGC TAAGAGCTCA TGCAAAATTG ACTACTTTTT ATGGAGCATT TGGACCAGAA 
AAATTCAGTG GCAGTTCTTG GATTGAATTT CTAAATAATG AAGATGATCT TAAAGATATT 
TTTTTACAGC TAAAAGAAGG AAACCTTGTT TGTGCACAGT ATCTTTGGCT TCGACATCGG 
GCAAACTTTG AAAGCAGATT TGATGTGAAA ATGCTGGAGA GCTTGCTCAA CTCAATGTCT 
GCATCAGTCT CTTTGCAAAA GCTGTGTCCA TGGTTTAAAA ATGATGTGAT TCCATTTGTA 
AGAAGGACTG TGCCTGAAGG ACAGATAATT CTTGCAAAAT GGTTGGAACA AGCAGCCAGG 
AACCTTGAAT TAACTGATAA GGCAAATTGG CCAGAAAATG GACTTCAATT GGCAGAGATA 
TTTTTTACAG CAGAAAAAAC AGACGAGTTG GGATTGGCAT CTTCCTGGCA TTGGATTTCC 
TTGAAAGATT ATCAGAACAC AGAGGAAGTA TGTCAGCTAA GGACTTTGGT AAATAACTTG 
CGAGAGTTGA TCACGTTGCA TAGGAAGTAC AACTGCAAAT TAGCCCTCTC TGATTTTGAG 
AAGGAAAATA CAACCACCAT AGTGTTCCGA ATGTTTGATA AAGTGCTGGC CCCAGAGCTT 
ATTCCCTCCA TCTTAGAGAA GTTTATAAGA GTTTACATGA GAGAACATGA CTTGCAAGAG 
GAGGAACTTC TCTTGCTGTA CATAGAGGAT TTACTGAATA GATGCAGCTC AAAGTCCACA 
TCACTCTTTG AAACAGCATG GGAAGCAAAG GCCATGGCAG TAATAGCGTG TTTATCTGAC 
ACGGACCTCA TATTTGATGC CGTGCTCAAG ATCATGTATG CGGCAGTGGT TCCTTGGAGT 
GCAGCTGTGG AGCAACTGGT GAAACAGCAC CTGGAAATGG ACCATCCCAA AGTCAAGTTA 
TTACAGGAAA GTTACAAACT AATGGAGATG AAAAAACTTT TACGAGGCTA TGGAATAAGA 
GAGGTAAATC TCTTAAACAA GGAAATAATG AGAGTGGTTA GATACATTCT CAAACAAGAT 
GTCCCATCTT CTTTAGAAGA TGCTTTAAAG GTAGCCCAAG CGTTTATGTT ATCTGATGAT 
GAGATCTACA GTCTAAGAAT TATTGACCTG ATTGATAGAG AACAGGGTGA AGACTGTCTC 
CTTCTGTTGA AGTCTTTGCC TCCTGCTGAA GCTGAGAAAA CTGCAGAAAG AGTCATCATA 
TGGGCACGAC TGGCATTACA AGAAGAGCCA GATCATTCTA AAGAGGGCAA GGCCTGGAGA 
ATGTCTGTAG CGAAGACATC CGTGGACATT CTTAAGATAC TATGTGACAT TCAGAAAGAC 
AATCTGCAGA AGAAGGACGA ATGTGAAGAA ATGTTGAAAC TATTTAAAGA GGTTGCTAGC 
TTACAGGAGA ACTTTGAGGT CTTTCTTTCA TTTGAAGATT ATAGCAATAG TTCCCTGGTA 
GCAGATCTCC GTGAGCAGCA CATTAAAGCT CACGAAGTTG CACAGGCGAA ACACAAACCT 
GGGAGCACCC CAGAGCCCAT AGCTGCTGAG GTGAGGAGCC CAAGCATGGA ATCAAAGCTG 
CACAGACAGG CACTGGCCCT GCAGATGTCC AAACAAGAGC TGGAGGCAGA GCTGACCTTG 
AGAGCCTTAA AAGATGGGAA CATCAAAACA GCACTGAAAA AATGCAGCGA CTTGTTTAAG 
TATCACTGCA ATGCTGACAC TGGGAAATTG CTATTTCTGA CATGTCAGAA GCTTTGTCAG 
ATGTTGGCTG ATAATGTCCC AGTGACAGTG CCTGTGGGAC TGAATCTTCC TTCCATGATA 
CATGATCTAG CAAGCCAAGC TGCCACCATT TGCAGTCCAG ATTTTTTACT AGATGCTTTA 
GAACTATGTA AACATACTTT AATGGCTGTA GAGCTTTCCA GACAATGCCA AATGGATGAC 
TGTGGAATCC TCATGAAAGC TTCTTTTGGG ACACATAAAG ATCCATATGA AGAGTGGTCT 
TACAGTGACT TCTTCAGTGA AGATGGAATT GTTCTTGAGT CACAGATGGT GCTTCCAGTG 
ATTTATGAAC TGATTTCATC TCTTGTGCCT CTAGCTGAAA GCAAGAGATA TCCCTTGGAG 
TCTACCAGTT TGCCATACTG CTCCCTTAAT GAAGGAGATG GCCTTGTTTT ACCTGTTATA 
AATTCCATCT CTGCCCTGCT TCAGAATCTT CAGGAATCTA GCCAGTGGGA GCTAGCCCTA 
AGATTTGTGG TTGGTTCATT TGGTACCTGT CTTCAGCACT CTGTGTCAAA CTTCATGAAT 
GCCACTTTGA GTGAAAAGTT ATTTGGAGAG ACTACATTAG TTAAATCAAG GCATGTTGTT 
ATGGAATTGA AAGAAAAAGC TGTTATATTT ATCAGGGAAA ATGCTACAAC ACTACTGCAC 
AAAGTATTTA ATTGTCGCTT GGTAGATCTT GACCTGGCGT TGGGTTACTG CACTCTCTTA 
CCTCAAAAAG ATGTGTTTGA AAATCTCTGG AAGCTCATAG ATAAAGCATG GCAGAATTAC 
GACAAAATCT TGGCAATATC TCTGGTGGGC TCTGAGCTGG CAAGTCTCTA TCAGGAAATA 
GAAATGGGGC TTAAGTTCCG TGAACTCAGT ACTGATGCCC AGTGGGGCAT TCGTCTTGGT 
AAACTTGGTA TTTCTTTTCA ACCAGTTTTC AGGCAACATT TTCTCACCAA GAAAGACCTC 
ATTAAAGCTC TTGTGGAGAA TATAGATATG GACACAAGCC TCATTTTGGA ATATTGCAGC 
ACATTTCAGT TGGACTGCGA TGCAGTTCTT CAGCTCTTCA TTGAAACGCT GCTCCACAAC 
ACAAATGCCG GCCAAGGCCA GGGAGATGCA AGCATGGACT CTGCAAAGCG GCGGCATCCC 
AAACTCCTGG CCAAAGCCCT TGAGATGGTT CCTTTACTGA CGAGCACAAA AGATTTGGTC 
ATCAGTCTTA GTGGAATACT ACATAAGTTG GATCCTTATG ACTATGAAAT GATTGAAGTT 
GTCTTGAAAG TTATAGAACG AGCTGATGAA AAGATAACCA ATATTAATAT TAATCAGGCA 
TTGAGTATTC TGAAACATTT GAAGTCATAC AGAAGAATTT CTCCTCCCGT GGATCTAGAA 
TATCAGTATA TGTTGGAACA TGTCATAACT TTGCCATCAG CTGCCCAAAC TAGACTGCCT 
TTTCACCTGA TATTCTTTGG CACAGCACAG AACTTCTGGA AAATTCTCTC TACAGAACTC 
AGTGAAGAAT CTTTCCCAAC ATTGCTCTTA ATTTCGAAAT TAATGAAGTT CTCTCTGGAC 
ACTCTGTACG TGTCTACAGC AAAACACGTT TTCGAAAAAA AACTGAAGCC AAAGCTCCTG 
AAGTTAACAC AAGCTAAATC CTCAACACTG ATTAACAAGG AAATAACTAA GATCACGCAG 
ACCATCGAAT CCTGCTTACT CTCTATAGTC AACCCAGAGT GGGCTGTAGC TATTGCCATC 
AGCCTTGCCC AGGATATCCC TGAAGGTTCC TTCAAGATAT CTGCTTTGAA ATTCTGCCTT 
TATTTAGCTG AGAGATGGCT ACAGAATATC CCATCGCAGG ACGAAAAACG TGAAAAAGCC 
GAGGCTTTGT TGAAGAAGCT TCATATCCAG TACCGGCGAT CGGGCACAGA AGCTGTGCTC 
ATAGCCCACA AGCTGAACAC TGAGGAATAT TTAAGAGTGA TCGGAAAGCC AGCACATCTT 
ATTGTCAGTC TCTACGAACA TCCTAGCATC AATCAAAGAA TTCAGAATTC ATCTGGCACA 
GATTATCCTG ATATTCATGC AGCAGCTAAA GAAATAGCCG AAGTCAATGA AATTAATTTG 
GAAAAAGTCT GGGACATGTT GTTGGAAAAA TGGCTATGCC CTTCAACAAA ACCTGGTGAA 
AAACCATCAG AATTATTTGA ACTTCAAGAA GATGAAGCCC TACGAAGAGT GCAGTATCTC 
CTCCTGTCTC GTCCAATTGA TTATAGTTCA AGAATGCTGT TTGTATTTGC AACATCAACT 
ACAACCACAT TAGGTATGCA TCAGTTAACT TTTGCCCATA GAACTCGAGC TCTTCAGTGT 
CTCTTCTATT TGGCTGACAA GGAAACTATA GAATCTCTCT TTAAAAAACC CATTGAAGAA 
GTGAAATCTT ATTTGAGATG TATAACTTTT CTGGCATCAT TTGAGACTTT GAATATCCCC 
ATCACATATG AATTATTTTG CAGCAGTCCT AAAGAAGGAA TGATTAAGGG TCTGTGGAAA 
AACCACAGCC ACGAGTCCAT GGCAGTAAGA TTGGTGACTG AGCTGTGTTT AGAATACAAA 
ATCTATGACC TGCAGCTTTG GAATGGACTC TTGCAAAAGC TTCTGGGCTT CAATATGATT 
CCTTATCTAA GGAAAGTTTT AAAAGCCATC TCCAGTATCC ATTCTTTATG GCAGGTTCCC 
TACTTCAGCA AAGCGTGGCA GCGTGTGATA CAGATACCAC TGCTTTCAGC CTCTTGTCCT 
TTAAGTCCTG ATCAGCTGTC AGATTGTTCT GAGAGTCTCA TCGCTGTCCT CGAATGTCCA 
GTCTCAGGTG ATCTTGACCT GATCGGAGTC GCCAGGCAGT ATATCCAGTT AGAACTTCCG 
GCTTTTGCAT TAGCTTGTCT GATGCTCATG CCCCACTCAG AGAAAAGACA CCAGCAAATT 
AAGAATTTTC TGGGTTCCTG TGACCCTCAG GTTATTTTAA AGCAATTGGA AGAGCATATG 
AACACGGGCC AGCTAGCAGG ATTTTCACAT CAAATTAGAA GTCTGATTTT GAATAATATC 
ATCAATAAGA AGGAGTTTGG GATTTTGGCA AAGACCAAAT ACTTTCAAAT GTTGAAGATG 
CATGCGATGA ATACCAACAA TATCACTGAG CTAGTGAACT ATTTGGCAAA TGACTTAAGT 
TTAGATGAAG CTTCAGTCTT GATAACTGAA TATTCAAAGC ACTGCGGGAA ACCTGTGCCT 
CCAGACACTG CTCCCTGTGA AATTCTGAAG ATGTTTCTTA GTGGATTATC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNTC1 ( XM_017020255.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875744.1 Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X4, mRNA.

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CloneID	OHu84341
Clone ID Related Accession (Same CDS sequence)	XM_017020255.2 , XM_017020255.1
Accession Version	XM_017020255.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	kinetochore-associated protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACTTC TTACATACAG TGGATTTTTT TGTATTACAA ACCTTCAGCT TTTAAAAATT 
CAACAAGCAA TTGAGAATGT AGACTTCAGT ACAGCAAAAA AGTTACAAGG ACAAATCAAG 
TCCAGTTTTA TTTCTACTGA AAATTATCAT ACTCTTGGTT GTCTCAGTCT TGTGGCTGGA 
GATTTAGCAA GTGAAGTTCC TGTGATAATT GGGGGAACCG GTAATTGTGC ATTCTCAAAA 
TGGGAACCAG ATTCTTCCAA GAAAGGAATG ACAGTTAAGA ACCTTATTGA TGCAGAGATT 
ATTAAAGGTG CAAAGAAGTT CCAGCTGATA GACAATCTAC TTTTTGTTCT TGATACTGAT 
AACGTGCTGA GTTTATGGGA TATTTACACT CTAACTCCTG TATGGAACTG GCCCTCTCTT 
CACGTAGAAG AGTTTCTTCT TACTACAGAA GCAGACTCTC CTTCATCAGT CACGTGGCAA 
GGAATTACAA ATCTCAAATT AATAGCTCTG ACAGCTTCAG CTAATAAGAA GATGAAAAAC 
CTCATGGTTT ATTCATTACC TACAATGGAA ATACTATATT CTTTGGAAGT ATCTAGTGTT 
TCTTCTCTGG TCCAAACAGG AATTAGCACA GATACCATAT ACCTTTTAGA AGGAGTTTGC 
AAAAATGATC CAAAATTGTC TGAAGACTCA GTCTCTGTGT TAGTACTCAG ATGTCTTACG 
GAAGCTTTAC CAGAAAACAG ATTGAGTCGG TTACTTCACA AACACAGATT TGCTGAAGCT 
GAGAGTTTTG CCATTCAGTT TGGACTAGAT GTTGAGCTTG TTTACAAGGT CAAGTCAAAT 
CATATATTGG AGAAACTGGC ATTGAGTTCT GTGGATGCCA GTGAACAGAC CGAATGGCAA 
CAACTTGTAG ACGACGCTAA GGAAAATCTA CATAAGATCC AGGATGATGA ATTTGTGGTG 
AATTACTGCC TGAAAGCTCA GTGGATAACC TATGAAACCA CTCAAGAGAT GCTGAATTAT 
GCCAAAACCA GGCTTTTGAA GAAAGAAGAT AAAACTGCTC TCATTTATTC TGATGGCTTG 
AAAGAGGTGC TAAGAGCTCA TGCAAAATTG ACTACTTTTT ATGGAGCATT TGGACCAGAA 
AAATTCAGTG GCAGTTCTTG GATTGAATTT CTAAATAATG AAGATGATCT TAAAGATATT 
TTTTTACAGC TAAAAGAAGG AAACCTTGTT TGTGCACAGT ATCTTTGGCT TCGACATCGG 
GCAAACTTTG AAAGCAGATT TGATGTGAAA ATGCTGGAGA GCTTGCTCAA CTCAATGTCT 
GCATCAGTCT CTTTGCAAAA GCTGTGTCCA TGGTTTAAAA ATGATGTGAT TCCATTTGTA 
AGAAGGACTG TGCCTGAAGG ACAGATAATT CTTGCAAAAT GGTTGGAACA AGCAGCCAGG 
AACCTTGAAT TAACTGATAA GGCAAATTGG CCAGAAAATG GACTTCAATT GGCAGAGATA 
TTTTTTACAG CAGAAAAAAC AGACGAGTTG GGATTGGCAT CTTCCTGGCA TTGGATTTCC 
TTGAAAGATT ATCAGAACAC AGAGGAAGTA TGTCAGCTAA GGACTTTGGT AAATAACTTG 
CGAGAGTTGA TCACGTTGCA TAGGAAGTAC AACTGCAAAT TAGCCCTCTC TGATTTTGAG 
AAGGAAAATA CAACCACCAT AGTGTTCCGA ATGTTTGATA AAGTGCTGGC CCCAGAGCTT 
ATTCCCTCCA TCTTAGAGAA GTTTATAAGA GTTTACATGA GAGAACATGA CTTGCAAGAG 
GAGGAACTTC TCTTGCTGTA CATAGAGGAT TTACTGAATA GATGCAGCTC AAAGTCCACA 
TCACTCTTTG AAACAGCATG GGAAGCAAAG GCCATGGCAG TAATAGCGTG TTTATCTGAC 
ACGGACCTCA TATTTGATGC CGTGCTCAAG ATCATGTATG CGGCAGTGGT TCCTTGGAGT 
GCAGCTGTGG AGCAACTGGT GAAACAGCAC CTGGAAATGG ACCATCCCAA AGTCAAGTTA 
TTACAGGAAA GTTACAAACT AATGGAGATG AAAAAACTTT TACGAGGCTA TGGAATAAGA 
GAGGTAAATC TCTTAAACAA GGAAATAATG AGAGTGGTTA GATACATTCT CAAACAAGAT 
GTCCCATCTT CTTTAGAAGA TGCTTTAAAG GTAGCCCAAG CGTTTATGTT ATCTGATGAT 
GAGATCTACA GTCTAAGAAT TATTGACCTG ATTGATAGAG AACAGGGTGA AGACTGTCTC 
CTTCTGTTGA AGTCTTTGCC TCCTGCTGAA GCTGAGAAAA CTGCAGAAAG AGTCATCATA 
TGGGCACGAC TGGCATTACA AGAAGAGCCA GATCATTCTA AAGAGGGCAA GGCCTGGAGA 
ATGTCTGTAG CGAAGACATC CGTGGACATT CTTAAGATAC TATGTGACAT TCAGAAAGAC 
AATCTGCAGA AGAAGGACGA ATGTGAAGAA ATGTTGAAAC TATTTAAAGA GGTTGCTAGC 
TTACAGGAGA ACTTTGAGGT CTTTCTTTCA TTTGAAGATT ATAGCAATAG TTCCCTGGTA 
GCAGATCTCC GTGAGCAGCA CATTAAAGCT CACGAAGTTG CACAGGCGAA ACACAAACCT 
GGGAGCACCC CAGAGCCCAT AGCTGCTGAG GTGAGGAGCC CAAGCATGGA ATCAAAGCTG 
CACAGACAGG CACTGGCCCT GCAGATGTCC AAACAAGAGC TGGAGGCAGA GCTGACCTTG 
AGAGCCTTAA AAGATGGGAA CATCAAAACA GCACTGAAAA AATGCAGCGA CTTGTTTAAG 
TATCACTGCA ATGCTGACAC TGGGAAATTG CTATTTCTGA CATGTCAGAA GCTTTGTCAG 
ATGTTGGCTG ATAATGTCCC AGTGACAGTG CCTGTGGGAC TGAATCTTCC TTCCATGATA 
CATGATCTAG CAAGCCAAGC TGCCACCATT TGCAGTCCAG ATTTTTTACT AGATGCTTTA 
GAACTATGTA AACATACTTT AATGGCTGTA GAGCTTTCCA GACAATGCCA AATGGATGAC 
TGTGGAATCC TCATGAAAGC TTCTTTTGGG ACACATAAAG ATCCATATGA AGAGTGGTCT 
TACAGTGACT TCTTCAGTGA AGATGGAATT GTTCTTGAGT CACAGATGGT GCTTCCAGTG 
ATTTATGAAC TGATTTCATC TCTTGTGCCT CTAGCTGAAA GCAAGAGATA TCCCTTGGAG 
TCTACCAGTT TGCCATACTG CTCCCTTAAT GAAGGAGATG GCCTTGTTTT ACCTGTTATA 
AATTCCATCT CTGCCCTGCT TCAGAATCTT CAGGAATCTA GCCAGTGGGA GCTAGCCCTA 
AGATTTGTGG TTGGTTCATT TGGTACCTGT CTTCAGCACT CTGTGTCAAA CTTCATGAAT 
GCCACTTTGA GTGAAAAGTT ATTTGGAGAG ACTACATTAG TTAAATCAAG GCATGTTGTT 
ATGGAATTGA AAGAAAAAGC TGTTATATTT ATCAGGGAAA ATGCTACAAC ACTACTGCAC 
AAAGTATTTA ATTGTCGCTT GGTAGATCTT GACCTGGCGT TGGGTTACTG CACTCTCTTA 
CCTCAAAAAG ATGTGTTTGA AAATCTCTGG AAGCTCATAG ATAAAGCATG GCAGAATTAC 
GACAAAATCT TGGCAATATC TCTGGTGGGC TCTGAGCTGG CAAGTCTCTA TCAGGAAATA 
GAAATGGGGC TTAAGTTCCG TGAACTCAGT ACTGATGCCC AGTGGGGCAT TCGTCTTGGT 
AAACTTGGTA TTTCTTTTCA ACCAGTTTTC AGGCAACATT TTCTCACCAA GAAAGACCTC 
ATTAAAGCTC TTGTGGAGAA TATAGATATG GACACAAGCC TCATTTTGGA ATATTGCAGC 
ACATTTCAGT TGGACTGCGA TGCAGTTCTT CAGCTCTTCA TTGAAACGCT GCTCCACAAC 
ACAAATGCCG GCCAAGGCCA GGGAGATGCA AGCATGGACT CTGCAAAGCG GCGGCATCCC 
AAACTCCTGG CCAAAGCCCT TGAGATGGTT CCTTTACTGA CGAGCACAAA AGATTTGGTC 
ATCAGTCTTA GTGGAATACT ACATAAGTTG GATCCTTATG ACTATGAAAT GATTGAAGTT 
GTCTTGAAAG TTATAGAACG AGCTGATGAA AAGATAACCA ATATTAATAT TAATCAGGCA 
TTGAGTATTC TGAAACATTT GAAGTCATAC AGAAGAATTT CTCCTCCCGT GGATCTAGAA 
TATCAGTATA TGTTGGAACA TGTCATAACT TTGCCATCAG CTGCCCAAAC TAGACTGCCT 
TTTCACCTGA TATTCTTTGG CACAGCACAG AACTTCTGGA AAATTCTCTC TACAGAACTC 
AGTGAAGAAT CTTTCCCAAC ATTGCTCTTA ATTTCGAAAT TAATGAAGTT CTCTCTGGAC 
ACTCTGTACG TGTCTACAGC AAAACACGTT TTCGAAAAAA AACTGAAGCC AAAGCTCCTG 
AAGTTAACAC AAGCTAAATC CTCAACACTG ATTAACAAGG AAATAACTAA GATCACGCAG 
ACCATCGAAT CCTGCTTACT CTCTATAGTC AACCCAGAGT GGGCTGTAGC TATTGCCATC 
AGCCTTGCCC AGGATATCCC TGAAGGTTCC TTCAAGATAT CTGCTTTGAA ATTCTGCCTT 
TATTTAGCTG AGAGATGGCT ACAGAATATC CCATCGCAGG ACGAAAAACG TGAAAAAGCC 
GAGGCTTTGT TGAAGAAGCT TCATATCCAG TACCGGCGAT CGGGCACAGA AGCTGTGCTC 
ATAGCCCACA AGCTGAACAC TGAGGAATAT TTAAGAGTGA TCGGAAAGCC AGCACATCTT 
ATTGTCAGTC TCTACGAACA TCCTAGCATC AATCAAAGAA TTCAGAATTC ATCTGGCACA 
GATTATCCTG ATATTCATGC AGCAGCTAAA GAAATAGCCG AAGTCAATGA AATTAATTTG 
GAAAAAGTCT GGGACATGTT GTTGGAAAAA TGGCTATGCC CTTCAACAAA ACCTGGTGAA 
AAACCATCAG AATTATTTGA ACTTCAAGAA GATGAAGCCC TACGAAGAGT GCAGTATCTC 
CTCCTGTCTC GTCCAATTGA TTATAGTTCA AGAATGCTGT TTGTATTTGC AACATCAACT 
ACAACCACAT TAGGTATGCA TCAGTTAACT TTTGCCCATA GAACTCGAGC TCTTCAGTGT 
CTCTTCTATT TGGCTGACAA GGAAACTATA GAATCTCTCT TTAAAAAACC CATTGAAGAA 
GTGAAATCTT ATTTGAGATG TATAACTTTT CTGGCATCAT TTGAGACTTT GAATATCCCC 
ATCACATATG AATTATTTTG CAGCAGTCCT AAAGAAGGAA TGATTAAGGG TCTGTGGAAA 
AACCACAGCC ACGAGTCCAT GGCAGTAAGA TTGGTGACTG AGCTGTGTTT AGAATACAAA 
ATCTATGACC TGCAGCTTTG GAATGGACTC TTGCAAAAGC TTCTGGGCTT CAATATGATT 
CCTTATCTAA GGAAAGTTTT AAAAGCCATC TCCAGTATCC ATTCTTTATG GCAGGTTCCC 
TACTTCAGCA AAGCGTGGCA GCGTGTGATA CAGATACCAC TGCTTTCAGC CTCTTGTCCT 
TTAAGTCCTG ATCAGCTGTC AGATTGTTCT GAGAGTCTCA TCGCTGTCCT CGAATGTCCA 
GTCTCAGGTG ATCTTGACCT GATCGGAGTC GCCAGGCAGT ATATCCAGTT AGAACTTCCG 
GCTTTTGCAT TAGCTTGTCT GATGCTCATG CCCCACTCAG AGAAAAGACA CCAGCAAATT 
AAGAATTTTC TGGGTTCCTG TGACCCTCAG GTTATTTTAA AGCAATTGGA AGAGCATATG 
AACACGGGCC AGCTAGCAGG ATTTTCACAT CAAATTAGAA GTCTGATTTT GAATAATATC 
ATCAATAAGA AGGAGTTTGG GATTTTGGCA AAGACCAAAT ACTTTCAAAT GTTGAAGATG 
CATGCGATGA ATACCAACAA TATCACTGAG CTAGTGAACT ATTTGGCAAA TGACTTAAGT 
TTAGATGAAG CTTCAGTCTT GATAACTGAA TATTCAAAGC ACTGCGGGAA ACCTGTGCCT 
CCAGACACTG CTCCCTGTGA AATTCTGAAG ATGTTTCTTA GTGGATTATC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875744.1
CDS	566..6739
Translation	MLLLTYSGFFCITNLQLLKIQQAIENVDFSTAKKLQGQIKSSFISTENYHTLGCLSLVAGDLASEVPVIIGGTGNCAFSKWEPDSSKKGMTVKNLIDAEIIKGAKKFQLIDNLLFVLDTDNVLSLWDIYTLTPVWNWPSLHVEEFLLTTEADSPSSVTWQGITNLKLIALTASANKKMKNLMVYSLPTMEILYSLEVSSVSSLVQTGISTDTIYLLEGVCKNDPKLSEDSVSVLVLRCLTEALPENRLSRLLHKHRFAEAESFAIQFGLDVELVYKVKSNHILEKLALSSVDASEQTEWQQLVDDAKENLHKIQDDEFVVNYCLKAQWITYETTQEMLNYAKTRLLKKEDKTALIYSDGLKEVLRAHAKLTTFYGAFGPEKFSGSSWIEFLNNEDDLKDIFLQLKEGNLVCAQYLWLRHRANFESRFDVKMLESLLNSMSASVSLQKLCPWFKNDVIPFVRRTVPEGQIILAKWLEQAARNLELTDKANWPENGLQLAEIFFTAEKTDELGLASSWHWISLKDYQNTEEVCQLRTLVNNLRELITLHRKYNCKLALSDFEKENTTTIVFRMFDKVLAPELIPSILEKFIRVYMREHDLQEEELLLLYIEDLLNRCSSKSTSLFETAWEAKAMAVIACLSDTDLIFDAVLKIMYAAVVPWSAAVEQLVKQHLEMDHPKVKLLQESYKLMEMKKLLRGYGIREVNLLNKEIMRVVRYILKQDVPSSLEDALKVAQAFMLSDDEIYSLRIIDLIDREQGEDCLLLLKSLPPAEAEKTAERVIIWARLALQEEPDHSKEGKAWRMSVAKTSVDILKILCDIQKDNLQKKDECEEMLKLFKEVASLQENFEVFLSFEDYSNSSLVADLREQHIKAHEVAQAKHKPGSTPEPIAAEVRSPSMESKLHRQALALQMSKQELEAELTLRALKDGNIKTALKKCSDLFKYHCNADTGKLLFLTCQKLCQMLADNVPVTVPVGLNLPSMIHDLASQAATICSPDFLLDALELCKHTLMAVELSRQCQMDDCGILMKASFGTHKDPYEEWSYSDFFSEDGIVLESQMVLPVIYELISSLVPLAESKRYPLESTSLPYCSLNEGDGLVLPVINSISALLQNLQESSQWELALRFVVGSFGTCLQHSVSNFMNATLSEKLFGETTLVKSRHVVMELKEKAVIFIRENATTLLHKVFNCRLVDLDLALGYCTLLPQKDVFENLWKLIDKAWQNYDKILAISLVGSELASLYQEIEMGLKFRELSTDAQWGIRLGKLGISFQPVFRQHFLTKKDLIKALVENIDMDTSLILEYCSTFQLDCDAVLQLFIETLLHNTNAGQGQGDASMDSAKRRHPKLLAKALEMVPLLTSTKDLVISLSGILHKLDPYDYEMIEVVLKVIERADEKITNININQALSILKHLKSYRRISPPVDLEYQYMLEHVITLPSAAQTRLPFHLIFFGTAQNFWKILSTELSEESFPTLLLISKLMKFSLDTLYVSTAKHVFEKKLKPKLLKLTQAKSSTLINKEITKITQTIESCLLSIVNPEWAVAIAISLAQDIPEGSFKISALKFCLYLAERWLQNIPSQDEKREKAEALLKKLHIQYRRSGTEAVLIAHKLNTEEYLRVIGKPAHLIVSLYEHPSINQRIQNSSGTDYPDIHAAAKEIAEVNEINLEKVWDMLLEKWLCPSTKPGEKPSELFELQEDEALRRVQYLLLSRPIDYSSRMLFVFATSTTTTLGMHQLTFAHRTRALQCLFYLADKETIESLFKKPIEEVKSYLRCITFLASFETLNIPITYELFCSSPKEGMIKGLWKNHSHESMAVRLVTELCLEYKIYDLQLWNGLLQKLLGFNMIPYLRKVLKAISSIHSLWQVPYFSKAWQRVIQIPLLSASCPLSPDQLSDCSESLIAVLECPVSGDLDLIGVARQYIQLELPAFALACLMLMPHSEKRHQQIKNFLGSCDPQVILKQLEEHMNTGQLAGFSHQIRSLILNNIINKKEFGILAKTKYFQMLKMHAMNTNNITELVNYLANDLSLDEASVLITEYSKHCGKPVPPDTAPCEILKMFLSGLS

Target ORF information:

RefSeq Version	XM_017020255.1
Organism	Homo sapiens(human)
Definition	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020255.1

ORF Insert Sequence:

ATGCTACTTC TTACATACAG TGGATTTTTT TGTATTACAA ACCTTCAGCT TTTAAAAATT 
CAACAAGCAA TTGAGAATGT AGACTTCAGT ACAGCAAAAA AGTTACAAGG ACAAATCAAG 
TCCAGTTTTA TTTCTACTGA AAATTATCAT ACTCTTGGTT GTCTCAGTCT TGTGGCTGGA 
GATTTAGCAA GTGAAGTTCC TGTGATAATT GGGGGAACCG GTAATTGTGC ATTCTCAAAA 
TGGGAACCAG ATTCTTCCAA GAAAGGAATG ACAGTTAAGA ACCTTATTGA TGCAGAGATT 
ATTAAAGGTG CAAAGAAGTT CCAGCTGATA GACAATCTAC TTTTTGTTCT TGATACTGAT 
AACGTGCTGA GTTTATGGGA TATTTACACT CTAACTCCTG TATGGAACTG GCCCTCTCTT 
CACGTAGAAG AGTTTCTTCT TACTACAGAA GCAGACTCTC CTTCATCAGT CACGTGGCAA 
GGAATTACAA ATCTCAAATT AATAGCTCTG ACAGCTTCAG CTAATAAGAA GATGAAAAAC 
CTCATGGTTT ATTCATTACC TACAATGGAA ATACTATATT CTTTGGAAGT ATCTAGTGTT 
TCTTCTCTGG TCCAAACAGG AATTAGCACA GATACCATAT ACCTTTTAGA AGGAGTTTGC 
AAAAATGATC CAAAATTGTC TGAAGACTCA GTCTCTGTGT TAGTACTCAG ATGTCTTACG 
GAAGCTTTAC CAGAAAACAG ATTGAGTCGG TTACTTCACA AACACAGATT TGCTGAAGCT 
GAGAGTTTTG CCATTCAGTT TGGACTAGAT GTTGAGCTTG TTTACAAGGT CAAGTCAAAT 
CATATATTGG AGAAACTGGC ATTGAGTTCT GTGGATGCCA GTGAACAGAC CGAATGGCAA 
CAACTTGTAG ACGACGCTAA GGAAAATCTA CATAAGATCC AGGATGATGA ATTTGTGGTG 
AATTACTGCC TGAAAGCTCA GTGGATAACC TATGAAACCA CTCAAGAGAT GCTGAATTAT 
GCCAAAACCA GGCTTTTGAA GAAAGAAGAT AAAACTGCTC TCATTTATTC TGATGGCTTG 
AAAGAGGTGC TAAGAGCTCA TGCAAAATTG ACTACTTTTT ATGGAGCATT TGGACCAGAA 
AAATTCAGTG GCAGTTCTTG GATTGAATTT CTAAATAATG AAGATGATCT TAAAGATATT 
TTTTTACAGC TAAAAGAAGG AAACCTTGTT TGTGCACAGT ATCTTTGGCT TCGACATCGG 
GCAAACTTTG AAAGCAGATT TGATGTGAAA ATGCTGGAGA GCTTGCTCAA CTCAATGTCT 
GCATCAGTCT CTTTGCAAAA GCTGTGTCCA TGGTTTAAAA ATGATGTGAT TCCATTTGTA 
AGAAGGACTG TGCCTGAAGG ACAGATAATT CTTGCAAAAT GGTTGGAACA AGCAGCCAGG 
AACCTTGAAT TAACTGATAA GGCAAATTGG CCAGAAAATG GACTTCAATT GGCAGAGATA 
TTTTTTACAG CAGAAAAAAC AGACGAGTTG GGATTGGCAT CTTCCTGGCA TTGGATTTCC 
TTGAAAGATT ATCAGAACAC AGAGGAAGTA TGTCAGCTAA GGACTTTGGT AAATAACTTG 
CGAGAGTTGA TCACGTTGCA TAGGAAGTAC AACTGCAAAT TAGCCCTCTC TGATTTTGAG 
AAGGAAAATA CAACCACCAT AGTGTTCCGA ATGTTTGATA AAGTGCTGGC CCCAGAGCTT 
ATTCCCTCCA TCTTAGAGAA GTTTATAAGA GTTTACATGA GAGAACATGA CTTGCAAGAG 
GAGGAACTTC TCTTGCTGTA CATAGAGGAT TTACTGAATA GATGCAGCTC AAAGTCCACA 
TCACTCTTTG AAACAGCATG GGAAGCAAAG GCCATGGCAG TAATAGCGTG TTTATCTGAC 
ACGGACCTCA TATTTGATGC CGTGCTCAAG ATCATGTATG CGGCAGTGGT TCCTTGGAGT 
GCAGCTGTGG AGCAACTGGT GAAACAGCAC CTGGAAATGG ACCATCCCAA AGTCAAGTTA 
TTACAGGAAA GTTACAAACT AATGGAGATG AAAAAACTTT TACGAGGCTA TGGAATAAGA 
GAGGTAAATC TCTTAAACAA GGAAATAATG AGAGTGGTTA GATACATTCT CAAACAAGAT 
GTCCCATCTT CTTTAGAAGA TGCTTTAAAG GTAGCCCAAG CGTTTATGTT ATCTGATGAT 
GAGATCTACA GTCTAAGAAT TATTGACCTG ATTGATAGAG AACAGGGTGA AGACTGTCTC 
CTTCTGTTGA AGTCTTTGCC TCCTGCTGAA GCTGAGAAAA CTGCAGAAAG AGTCATCATA 
TGGGCACGAC TGGCATTACA AGAAGAGCCA GATCATTCTA AAGAGGGCAA GGCCTGGAGA 
ATGTCTGTAG CGAAGACATC CGTGGACATT CTTAAGATAC TATGTGACAT TCAGAAAGAC 
AATCTGCAGA AGAAGGACGA ATGTGAAGAA ATGTTGAAAC TATTTAAAGA GGTTGCTAGC 
TTACAGGAGA ACTTTGAGGT CTTTCTTTCA TTTGAAGATT ATAGCAATAG TTCCCTGGTA 
GCAGATCTCC GTGAGCAGCA CATTAAAGCT CACGAAGTTG CACAGGCGAA ACACAAACCT 
GGGAGCACCC CAGAGCCCAT AGCTGCTGAG GTGAGGAGCC CAAGCATGGA ATCAAAGCTG 
CACAGACAGG CACTGGCCCT GCAGATGTCC AAACAAGAGC TGGAGGCAGA GCTGACCTTG 
AGAGCCTTAA AAGATGGGAA CATCAAAACA GCACTGAAAA AATGCAGCGA CTTGTTTAAG 
TATCACTGCA ATGCTGACAC TGGGAAATTG CTATTTCTGA CATGTCAGAA GCTTTGTCAG 
ATGTTGGCTG ATAATGTCCC AGTGACAGTG CCTGTGGGAC TGAATCTTCC TTCCATGATA 
CATGATCTAG CAAGCCAAGC TGCCACCATT TGCAGTCCAG ATTTTTTACT AGATGCTTTA 
GAACTATGTA AACATACTTT AATGGCTGTA GAGCTTTCCA GACAATGCCA AATGGATGAC 
TGTGGAATCC TCATGAAAGC TTCTTTTGGG ACACATAAAG ATCCATATGA AGAGTGGTCT 
TACAGTGACT TCTTCAGTGA AGATGGAATT GTTCTTGAGT CACAGATGGT GCTTCCAGTG 
ATTTATGAAC TGATTTCATC TCTTGTGCCT CTAGCTGAAA GCAAGAGATA TCCCTTGGAG 
TCTACCAGTT TGCCATACTG CTCCCTTAAT GAAGGAGATG GCCTTGTTTT ACCTGTTATA 
AATTCCATCT CTGCCCTGCT TCAGAATCTT CAGGAATCTA GCCAGTGGGA GCTAGCCCTA 
AGATTTGTGG TTGGTTCATT TGGTACCTGT CTTCAGCACT CTGTGTCAAA CTTCATGAAT 
GCCACTTTGA GTGAAAAGTT ATTTGGAGAG ACTACATTAG TTAAATCAAG GCATGTTGTT 
ATGGAATTGA AAGAAAAAGC TGTTATATTT ATCAGGGAAA ATGCTACAAC ACTACTGCAC 
AAAGTATTTA ATTGTCGCTT GGTAGATCTT GACCTGGCGT TGGGTTACTG CACTCTCTTA 
CCTCAAAAAG ATGTGTTTGA AAATCTCTGG AAGCTCATAG ATAAAGCATG GCAGAATTAC 
GACAAAATCT TGGCAATATC TCTGGTGGGC TCTGAGCTGG CAAGTCTCTA TCAGGAAATA 
GAAATGGGGC TTAAGTTCCG TGAACTCAGT ACTGATGCCC AGTGGGGCAT TCGTCTTGGT 
AAACTTGGTA TTTCTTTTCA ACCAGTTTTC AGGCAACATT TTCTCACCAA GAAAGACCTC 
ATTAAAGCTC TTGTGGAGAA TATAGATATG GACACAAGCC TCATTTTGGA ATATTGCAGC 
ACATTTCAGT TGGACTGCGA TGCAGTTCTT CAGCTCTTCA TTGAAACGCT GCTCCACAAC 
ACAAATGCCG GCCAAGGCCA GGGAGATGCA AGCATGGACT CTGCAAAGCG GCGGCATCCC 
AAACTCCTGG CCAAAGCCCT TGAGATGGTT CCTTTACTGA CGAGCACAAA AGATTTGGTC 
ATCAGTCTTA GTGGAATACT ACATAAGTTG GATCCTTATG ACTATGAAAT GATTGAAGTT 
GTCTTGAAAG TTATAGAACG AGCTGATGAA AAGATAACCA ATATTAATAT TAATCAGGCA 
TTGAGTATTC TGAAACATTT GAAGTCATAC AGAAGAATTT CTCCTCCCGT GGATCTAGAA 
TATCAGTATA TGTTGGAACA TGTCATAACT TTGCCATCAG CTGCCCAAAC TAGACTGCCT 
TTTCACCTGA TATTCTTTGG CACAGCACAG AACTTCTGGA AAATTCTCTC TACAGAACTC 
AGTGAAGAAT CTTTCCCAAC ATTGCTCTTA ATTTCGAAAT TAATGAAGTT CTCTCTGGAC 
ACTCTGTACG TGTCTACAGC AAAACACGTT TTCGAAAAAA AACTGAAGCC AAAGCTCCTG 
AAGTTAACAC AAGCTAAATC CTCAACACTG ATTAACAAGG AAATAACTAA GATCACGCAG 
ACCATCGAAT CCTGCTTACT CTCTATAGTC AACCCAGAGT GGGCTGTAGC TATTGCCATC 
AGCCTTGCCC AGGATATCCC TGAAGGTTCC TTCAAGATAT CTGCTTTGAA ATTCTGCCTT 
TATTTAGCTG AGAGATGGCT ACAGAATATC CCATCGCAGG ACGAAAAACG TGAAAAAGCC 
GAGGCTTTGT TGAAGAAGCT TCATATCCAG TACCGGCGAT CGGGCACAGA AGCTGTGCTC 
ATAGCCCACA AGCTGAACAC TGAGGAATAT TTAAGAGTGA TCGGAAAGCC AGCACATCTT 
ATTGTCAGTC TCTACGAACA TCCTAGCATC AATCAAAGAA TTCAGAATTC ATCTGGCACA 
GATTATCCTG ATATTCATGC AGCAGCTAAA GAAATAGCCG AAGTCAATGA AATTAATTTG 
GAAAAAGTCT GGGACATGTT GTTGGAAAAA TGGCTATGCC CTTCAACAAA ACCTGGTGAA 
AAACCATCAG AATTATTTGA ACTTCAAGAA GATGAAGCCC TACGAAGAGT GCAGTATCTC 
CTCCTGTCTC GTCCAATTGA TTATAGTTCA AGAATGCTGT TTGTATTTGC AACATCAACT 
ACAACCACAT TAGGTATGCA TCAGTTAACT TTTGCCCATA GAACTCGAGC TCTTCAGTGT 
CTCTTCTATT TGGCTGACAA GGAAACTATA GAATCTCTCT TTAAAAAACC CATTGAAGAA 
GTGAAATCTT ATTTGAGATG TATAACTTTT CTGGCATCAT TTGAGACTTT GAATATCCCC 
ATCACATATG AATTATTTTG CAGCAGTCCT AAAGAAGGAA TGATTAAGGG TCTGTGGAAA 
AACCACAGCC ACGAGTCCAT GGCAGTAAGA TTGGTGACTG AGCTGTGTTT AGAATACAAA 
ATCTATGACC TGCAGCTTTG GAATGGACTC TTGCAAAAGC TTCTGGGCTT CAATATGATT 
CCTTATCTAA GGAAAGTTTT AAAAGCCATC TCCAGTATCC ATTCTTTATG GCAGGTTCCC 
TACTTCAGCA AAGCGTGGCA GCGTGTGATA CAGATACCAC TGCTTTCAGC CTCTTGTCCT 
TTAAGTCCTG ATCAGCTGTC AGATTGTTCT GAGAGTCTCA TCGCTGTCCT CGAATGTCCA 
GTCTCAGGTG ATCTTGACCT GATCGGAGTC GCCAGGCAGT ATATCCAGTT AGAACTTCCG 
GCTTTTGCAT TAGCTTGTCT GATGCTCATG CCCCACTCAG AGAAAAGACA CCAGCAAATT 
AAGAATTTTC TGGGTTCCTG TGACCCTCAG GTTATTTTAA AGCAATTGGA AGAGCATATG 
AACACGGGCC AGCTAGCAGG ATTTTCACAT CAAATTAGAA GTCTGATTTT GAATAATATC 
ATCAATAAGA AGGAGTTTGG GATTTTGGCA AAGACCAAAT ACTTTCAAAT GTTGAAGATG 
CATGCGATGA ATACCAACAA TATCACTGAG CTAGTGAACT ATTTGGCAAA TGACTTAAGT 
TTAGATGAAG CTTCAGTCTT GATAACTGAA TATTCAAAGC ACTGCGGGAA ACCTGTGCCT 
CCAGACACTG CTCCCTGTGA AATTCTGAAG ATGTTTCTTA GTGGATTATC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNTC1 ( NM_014708.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_055523.1 Homo sapiens kinetochore associated 1 (KNTC1), mRNA.

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CloneID	OHu10474
Clone ID Related Accession (Same CDS sequence)	NM_014708.6 , NM_014708.5 , NM_014708.4 , XM_006719706.2
Accession Version	NM_014708.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	kinetochore-associated protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC127002.3 and AC026333.28. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_014708.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC150278.1, D79988.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000333479.12/ ENSP00000328236.6 RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAACCA 
GTTTTCAGGC AACATTTTCT CACCAAGAAA GACCTCATTA AAGCTCTTGT GGAGAATATA 
GATATGGACA CAAGCCTCAT TTTGGAATAT TGCAGCACAT TTCAGTTGGA CTGCGATGCA 
GTTCTTCAGC TCTTCATTGA AACGCTGCTC CACAACACAA ATGCCGGCCA AGGCCAGGGA 
GATGCAAGCA TGGACTCTGC AAAGCGGCGG CATCCCAAAC TCCTGGCCAA AGCCCTTGAG 
ATGGTTCCTT TACTGACGAG CACAAAAGAT TTGGTCATCA GTCTTAGTGG AATACTACAT 
AAGTTGGATC CTTATGACTA TGAAATGATT GAAGTTGTCT TGAAAGTTAT AGAACGAGCT 
GATGAAAAGA TAACCAATAT TAATATTAAT CAGGCATTGA GTATTCTGAA ACATTTGAAG 
TCATACAGAA GAATTTCTCC TCCCGTGGAT CTAGAATATC AGTATATGTT GGAACATGTC 
ATAACTTTGC CATCAGCTGC CCAAACTAGA CTGCCTTTTC ACCTGATATT CTTTGGCACA 
GCACAGAACT TCTGGAAAAT TCTCTCTACA GAACTCAGTG AAGAATCTTT CCCAACATTG 
CTCTTAATTT CGAAATTAAT GAAGTTCTCT CTGGACACTC TGTACGTGTC TACAGCAAAA 
CACGTTTTCG AAAAAAAACT GAAGCCAAAG CTCCTGAAGT TAACACAAGC TAAATCCTCA 
ACACTGATTA ACAAGGAAAT AACTAAGATC ACGCAGACCA TCGAATCCTG CTTACTCTCT 
ATAGTCAACC CAGAGTGGGC TGTAGCTATT GCCATCAGCC TTGCCCAGGA TATCCCTGAA 
GGTTCCTTCA AGATATCTGC TTTGAAATTC TGCCTTTATT TAGCTGAGAG ATGGCTACAG 
AATATCCCAT CGCAGGACGA AAAACGTGAA AAAGCCGAGG CTTTGTTGAA GAAGCTTCAT 
ATCCAGTACC GGCGATCGGG CACAGAAGCT GTGCTCATAG CCCACAAGCT GAACACTGAG 
GAATATTTAA GAGTGATCGG AAAGCCAGCA CATCTTATTG TCAGTCTCTA CGAACATCCT 
AGCATCAATC AAAGAATTCA GAATTCATCT GGCACAGATT ATCCTGATAT TCATGCAGCA 
GCTAAAGAAA TAGCCGAAGT CAATGAAATT AATTTGGAAA AAGTCTGGGA CATGTTGTTG 
GAAAAATGGC TATGCCCTTC AACAAAACCT GGTGAAAAAC CATCAGAATT ATTTGAACTT 
CAAGAAGATG AAGCCCTACG AAGAGTGCAG TATCTCCTCC TGTCTCGTCC AATTGATTAT 
AGTTCAAGAA TGCTGTTTGT ATTTGCAACA TCAACTACAA CCACATTAGG TATGCATCAG 
TTAACTTTTG CCCATAGAAC TCGAGCTCTT CAGTGTCTCT TCTATTTGGC TGACAAGGAA 
ACTATAGAAT CTCTCTTTAA AAAACCCATT GAAGAAGTGA AATCTTATTT GAGATGTATA 
ACTTTTCTGG CATCATTTGA GACTTTGAAT ATCCCCATCA CATATGAATT ATTTTGCAGC 
AGTCCTAAAG AAGGAATGAT TAAGGGTCTG TGGAAAAACC ACAGCCACGA GTCCATGGCA 
GTAAGATTGG TGACTGAGCT GTGTTTAGAA TACAAAATCT ATGACCTGCA GCTTTGGAAT 
GGACTCTTGC AAAAGCTTCT GGGCTTCAAT ATGATTCCTT ATCTAAGGAA AGTTTTAAAA 
GCCATCTCCA GTATCCATTC TTTATGGCAG GTTCCCTACT TCAGCAAAGC GTGGCAGCGT 
GTGATACAGA TACCACTGCT TTCAGCCTCT TGTCCTTTAA GTCCTGATCA GCTGTCAGAT 
TGTTCTGAGA GTCTCATCGC TGTCCTCGAA TGTCCAGTCT CAGGTGATCT TGACCTGATC 
GGAGTCGCCA GGCAGTATAT CCAGTTAGAA CTTCCGGCTT TTGCATTAGC TTGTCTGATG 
CTCATGCCCC ACTCAGAGAA AAGACACCAG CAAATTAAGA ATTTTCTGGG TTCCTGTGAC 
CCTCAGGTTA TTTTAAAGCA ATTGGAAGAG CATATGAACA CGGGCCAGCT AGCAGGATTT 
TCACATCAAA TTAGAAGTCT GATTTTGAAT AATATCATCA ATAAGAAGGA GTTTGGGATT 
TTGGCAAAGA CCAAATACTT TCAAATGTTG AAGATGCATG CGATGAATAC CAACAATATC 
ACTGAGCTAG TGAACTATTT GGCAAATGAC TTAAGTTTAG ATGAAGCTTC AGTCTTGATA 
ACTGAATATT CAAAGCACTG CGGGAAACCT GTGCCTCCAG ACACTGCTCC CTGTGAAATT 
CTGAAGATGT TTCTTAGTGG ATTATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055523.1
CDS	177..6806
Translation	MWNDIELLTNDDTGSGYLSVGSRKEHGTALYQVDLLVKISSEKASLNPKIQACSLSDGFIIVADQSVILLDSICRSLQLHLVFDTEVDVVGLCQEGKFLLVGERSGNLHLIHVTSKQTLLTNAFVQKANDENRRTYQNLVIEKDGSNEGTYYMLLLTYSGFFCITNLQLLKIQQAIENVDFSTAKKLQGQIKSSFISTENYHTLGCLSLVAGDLASEVPVIIGGTGNCAFSKWEPDSSKKGMTVKNLIDAEIIKGAKKFQLIDNLLFVLDTDNVLSLWDIYTLTPVWNWPSLHVEEFLLTTEADSPSSVTWQGITNLKLIALTASANKKMKNLMVYSLPTMEILYSLEVSSVSSLVQTGISTDTIYLLEGVCKNDPKLSEDSVSVLVLRCLTEALPENRLSRLLHKHRFAEAESFAIQFGLDVELVYKVKSNHILEKLALSSVDASEQTEWQQLVDDAKENLHKIQDDEFVVNYCLKAQWITYETTQEMLNYAKTRLLKKEDKTALIYSDGLKEVLRAHAKLTTFYGAFGPEKFSGSSWIEFLNNEDDLKDIFLQLKEGNLVCAQYLWLRHRANFESRFDVKMLESLLNSMSASVSLQKLCPWFKNDVIPFVRRTVPEGQIILAKWLEQAARNLELTDKANWPENGLQLAEIFFTAEKTDELGLASSWHWISLKDYQNTEEVCQLRTLVNNLRELITLHRKYNCKLALSDFEKENTTTIVFRMFDKVLAPELIPSILEKFIRVYMREHDLQEEELLLLYIEDLLNRCSSKSTSLFETAWEAKAMAVIACLSDTDLIFDAVLKIMYAAVVPWSAAVEQLVKQHLEMDHPKVKLLQESYKLMEMKKLLRGYGIREVNLLNKEIMRVVRYILKQDVPSSLEDALKVAQAFMLSDDEIYSLRIIDLIDREQGEDCLLLLKSLPPAEAEKTAERVIIWARLALQEEPDHSKEGKAWRMSVAKTSVDILKILCDIQKDNLQKKDECEEMLKLFKEVASLQENFEVFLSFEDYSNSSLVADLREQHIKAHEVAQAKHKPGSTPEPIAAEVRSPSMESKLHRQALALQMSKQELEAELTLRALKDGNIKTALKKCSDLFKYHCNADTGKLLFLTCQKLCQMLADNVPVTVPVGLNLPSMIHDLASQAATICSPDFLLDALELCKHTLMAVELSRQCQMDDCGILMKASFGTHKDPYEEWSYSDFFSEDGIVLESQMVLPVIYELISSLVPLAESKRYPLESTSLPYCSLNEGDGLVLPVINSISALLQNLQESSQWELALRFVVGSFGTCLQHSVSNFMNATLSEKLFGETTLVKSRHVVMELKEKAVIFIRENATTLLHKVFNCRLVDLDLALGYCTLLPQKDVFENLWKLIDKAWQNYDKILAISLVGSELASLYQEIEMGLKFRELSTDAQWGIRLGKLGISFQPVFRQHFLTKKDLIKALVENIDMDTSLILEYCSTFQLDCDAVLQLFIETLLHNTNAGQGQGDASMDSAKRRHPKLLAKALEMVPLLTSTKDLVISLSGILHKLDPYDYEMIEVVLKVIERADEKITNININQALSILKHLKSYRRISPPVDLEYQYMLEHVITLPSAAQTRLPFHLIFFGTAQNFWKILSTELSEESFPTLLLISKLMKFSLDTLYVSTAKHVFEKKLKPKLLKLTQAKSSTLINKEITKITQTIESCLLSIVNPEWAVAIAISLAQDIPEGSFKISALKFCLYLAERWLQNIPSQDEKREKAEALLKKLHIQYRRSGTEAVLIAHKLNTEEYLRVIGKPAHLIVSLYEHPSINQRIQNSSGTDYPDIHAAAKEIAEVNEINLEKVWDMLLEKWLCPSTKPGEKPSELFELQEDEALRRVQYLLLSRPIDYSSRMLFVFATSTTTTLGMHQLTFAHRTRALQCLFYLADKETIESLFKKPIEEVKSYLRCITFLASFETLNIPITYELFCSSPKEGMIKGLWKNHSHESMAVRLVTELCLEYKIYDLQLWNGLLQKLLGFNMIPYLRKVLKAISSIHSLWQVPYFSKAWQRVIQIPLLSASCPLSPDQLSDCSESLIAVLECPVSGDLDLIGVARQYIQLELPAFALACLMLMPHSEKRHQQIKNFLGSCDPQVILKQLEEHMNTGQLAGFSHQIRSLILNNIINKKEFGILAKTKYFQMLKMHAMNTNNITELVNYLANDLSLDEASVLITEYSKHCGKPVPPDTAPCEILKMFLSGLS

Target ORF information:

RefSeq Version	NM_014708.6
Organism	Homo sapiens(human)
Definition	Homo sapiens kinetochore associated 1 (KNTC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014708.6

ORF Insert Sequence:

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAACCA 
GTTTTCAGGC AACATTTTCT CACCAAGAAA GACCTCATTA AAGCTCTTGT GGAGAATATA 
GATATGGACA CAAGCCTCAT TTTGGAATAT TGCAGCACAT TTCAGTTGGA CTGCGATGCA 
GTTCTTCAGC TCTTCATTGA AACGCTGCTC CACAACACAA ATGCCGGCCA AGGCCAGGGA 
GATGCAAGCA TGGACTCTGC AAAGCGGCGG CATCCCAAAC TCCTGGCCAA AGCCCTTGAG 
ATGGTTCCTT TACTGACGAG CACAAAAGAT TTGGTCATCA GTCTTAGTGG AATACTACAT 
AAGTTGGATC CTTATGACTA TGAAATGATT GAAGTTGTCT TGAAAGTTAT AGAACGAGCT 
GATGAAAAGA TAACCAATAT TAATATTAAT CAGGCATTGA GTATTCTGAA ACATTTGAAG 
TCATACAGAA GAATTTCTCC TCCCGTGGAT CTAGAATATC AGTATATGTT GGAACATGTC 
ATAACTTTGC CATCAGCTGC CCAAACTAGA CTGCCTTTTC ACCTGATATT CTTTGGCACA 
GCACAGAACT TCTGGAAAAT TCTCTCTACA GAACTCAGTG AAGAATCTTT CCCAACATTG 
CTCTTAATTT CGAAATTAAT GAAGTTCTCT CTGGACACTC TGTACGTGTC TACAGCAAAA 
CACGTTTTCG AAAAAAAACT GAAGCCAAAG CTCCTGAAGT TAACACAAGC TAAATCCTCA 
ACACTGATTA ACAAGGAAAT AACTAAGATC ACGCAGACCA TCGAATCCTG CTTACTCTCT 
ATAGTCAACC CAGAGTGGGC TGTAGCTATT GCCATCAGCC TTGCCCAGGA TATCCCTGAA 
GGTTCCTTCA AGATATCTGC TTTGAAATTC TGCCTTTATT TAGCTGAGAG ATGGCTACAG 
AATATCCCAT CGCAGGACGA AAAACGTGAA AAAGCCGAGG CTTTGTTGAA GAAGCTTCAT 
ATCCAGTACC GGCGATCGGG CACAGAAGCT GTGCTCATAG CCCACAAGCT GAACACTGAG 
GAATATTTAA GAGTGATCGG AAAGCCAGCA CATCTTATTG TCAGTCTCTA CGAACATCCT 
AGCATCAATC AAAGAATTCA GAATTCATCT GGCACAGATT ATCCTGATAT TCATGCAGCA 
GCTAAAGAAA TAGCCGAAGT CAATGAAATT AATTTGGAAA AAGTCTGGGA CATGTTGTTG 
GAAAAATGGC TATGCCCTTC AACAAAACCT GGTGAAAAAC CATCAGAATT ATTTGAACTT 
CAAGAAGATG AAGCCCTACG AAGAGTGCAG TATCTCCTCC TGTCTCGTCC AATTGATTAT 
AGTTCAAGAA TGCTGTTTGT ATTTGCAACA TCAACTACAA CCACATTAGG TATGCATCAG 
TTAACTTTTG CCCATAGAAC TCGAGCTCTT CAGTGTCTCT TCTATTTGGC TGACAAGGAA 
ACTATAGAAT CTCTCTTTAA AAAACCCATT GAAGAAGTGA AATCTTATTT GAGATGTATA 
ACTTTTCTGG CATCATTTGA GACTTTGAAT ATCCCCATCA CATATGAATT ATTTTGCAGC 
AGTCCTAAAG AAGGAATGAT TAAGGGTCTG TGGAAAAACC ACAGCCACGA GTCCATGGCA 
GTAAGATTGG TGACTGAGCT GTGTTTAGAA TACAAAATCT ATGACCTGCA GCTTTGGAAT 
GGACTCTTGC AAAAGCTTCT GGGCTTCAAT ATGATTCCTT ATCTAAGGAA AGTTTTAAAA 
GCCATCTCCA GTATCCATTC TTTATGGCAG GTTCCCTACT TCAGCAAAGC GTGGCAGCGT 
GTGATACAGA TACCACTGCT TTCAGCCTCT TGTCCTTTAA GTCCTGATCA GCTGTCAGAT 
TGTTCTGAGA GTCTCATCGC TGTCCTCGAA TGTCCAGTCT CAGGTGATCT TGACCTGATC 
GGAGTCGCCA GGCAGTATAT CCAGTTAGAA CTTCCGGCTT TTGCATTAGC TTGTCTGATG 
CTCATGCCCC ACTCAGAGAA AAGACACCAG CAAATTAAGA ATTTTCTGGG TTCCTGTGAC 
CCTCAGGTTA TTTTAAAGCA ATTGGAAGAG CATATGAACA CGGGCCAGCT AGCAGGATTT 
TCACATCAAA TTAGAAGTCT GATTTTGAAT AATATCATCA ATAAGAAGGA GTTTGGGATT 
TTGGCAAAGA CCAAATACTT TCAAATGTTG AAGATGCATG CGATGAATAC CAACAATATC 
ACTGAGCTAG TGAACTATTT GGCAAATGAC TTAAGTTTAG ATGAAGCTTC AGTCTTGATA 
ACTGAATATT CAAAGCACTG CGGGAAACCT GTGCCTCCAG ACACTGCTCC CTGTGAAATT 
CTGAAGATGT TTCTTAGTGG ATTATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNTC1 ( NM_014708.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_055523.1 Homo sapiens kinetochore associated 1 (KNTC1), mRNA.

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CloneID	OHu10474
Clone ID Related Accession (Same CDS sequence)	NM_014708.6 , NM_014708.5 , NM_014708.4 , XM_006719706.2
Accession Version	NM_014708.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-09
Organism	Homo sapiens(human)
Product	kinetochore-associated protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC127002.3 and AC026333.28. This sequence is a reference standard in the RefSeqGene project. On Jul 5, 2017 this sequence version replaced NM_014708.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC150278.1, D79988.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAACCA 
GTTTTCAGGC AACATTTTCT CACCAAGAAA GACCTCATTA AAGCTCTTGT GGAGAATATA 
GATATGGACA CAAGCCTCAT TTTGGAATAT TGCAGCACAT TTCAGTTGGA CTGCGATGCA 
GTTCTTCAGC TCTTCATTGA AACGCTGCTC CACAACACAA ATGCCGGCCA AGGCCAGGGA 
GATGCAAGCA TGGACTCTGC AAAGCGGCGG CATCCCAAAC TCCTGGCCAA AGCCCTTGAG 
ATGGTTCCTT TACTGACGAG CACAAAAGAT TTGGTCATCA GTCTTAGTGG AATACTACAT 
AAGTTGGATC CTTATGACTA TGAAATGATT GAAGTTGTCT TGAAAGTTAT AGAACGAGCT 
GATGAAAAGA TAACCAATAT TAATATTAAT CAGGCATTGA GTATTCTGAA ACATTTGAAG 
TCATACAGAA GAATTTCTCC TCCCGTGGAT CTAGAATATC AGTATATGTT GGAACATGTC 
ATAACTTTGC CATCAGCTGC CCAAACTAGA CTGCCTTTTC ACCTGATATT CTTTGGCACA 
GCACAGAACT TCTGGAAAAT TCTCTCTACA GAACTCAGTG AAGAATCTTT CCCAACATTG 
CTCTTAATTT CGAAATTAAT GAAGTTCTCT CTGGACACTC TGTACGTGTC TACAGCAAAA 
CACGTTTTCG AAAAAAAACT GAAGCCAAAG CTCCTGAAGT TAACACAAGC TAAATCCTCA 
ACACTGATTA ACAAGGAAAT AACTAAGATC ACGCAGACCA TCGAATCCTG CTTACTCTCT 
ATAGTCAACC CAGAGTGGGC TGTAGCTATT GCCATCAGCC TTGCCCAGGA TATCCCTGAA 
GGTTCCTTCA AGATATCTGC TTTGAAATTC TGCCTTTATT TAGCTGAGAG ATGGCTACAG 
AATATCCCAT CGCAGGACGA AAAACGTGAA AAAGCCGAGG CTTTGTTGAA GAAGCTTCAT 
ATCCAGTACC GGCGATCGGG CACAGAAGCT GTGCTCATAG CCCACAAGCT GAACACTGAG 
GAATATTTAA GAGTGATCGG AAAGCCAGCA CATCTTATTG TCAGTCTCTA CGAACATCCT 
AGCATCAATC AAAGAATTCA GAATTCATCT GGCACAGATT ATCCTGATAT TCATGCAGCA 
GCTAAAGAAA TAGCCGAAGT CAATGAAATT AATTTGGAAA AAGTCTGGGA CATGTTGTTG 
GAAAAATGGC TATGCCCTTC AACAAAACCT GGTGAAAAAC CATCAGAATT ATTTGAACTT 
CAAGAAGATG AAGCCCTACG AAGAGTGCAG TATCTCCTCC TGTCTCGTCC AATTGATTAT 
AGTTCAAGAA TGCTGTTTGT ATTTGCAACA TCAACTACAA CCACATTAGG TATGCATCAG 
TTAACTTTTG CCCATAGAAC TCGAGCTCTT CAGTGTCTCT TCTATTTGGC TGACAAGGAA 
ACTATAGAAT CTCTCTTTAA AAAACCCATT GAAGAAGTGA AATCTTATTT GAGATGTATA 
ACTTTTCTGG CATCATTTGA GACTTTGAAT ATCCCCATCA CATATGAATT ATTTTGCAGC 
AGTCCTAAAG AAGGAATGAT TAAGGGTCTG TGGAAAAACC ACAGCCACGA GTCCATGGCA 
GTAAGATTGG TGACTGAGCT GTGTTTAGAA TACAAAATCT ATGACCTGCA GCTTTGGAAT 
GGACTCTTGC AAAAGCTTCT GGGCTTCAAT ATGATTCCTT ATCTAAGGAA AGTTTTAAAA 
GCCATCTCCA GTATCCATTC TTTATGGCAG GTTCCCTACT TCAGCAAAGC GTGGCAGCGT 
GTGATACAGA TACCACTGCT TTCAGCCTCT TGTCCTTTAA GTCCTGATCA GCTGTCAGAT 
TGTTCTGAGA GTCTCATCGC TGTCCTCGAA TGTCCAGTCT CAGGTGATCT TGACCTGATC 
GGAGTCGCCA GGCAGTATAT CCAGTTAGAA CTTCCGGCTT TTGCATTAGC TTGTCTGATG 
CTCATGCCCC ACTCAGAGAA AAGACACCAG CAAATTAAGA ATTTTCTGGG TTCCTGTGAC 
CCTCAGGTTA TTTTAAAGCA ATTGGAAGAG CATATGAACA CGGGCCAGCT AGCAGGATTT 
TCACATCAAA TTAGAAGTCT GATTTTGAAT AATATCATCA ATAAGAAGGA GTTTGGGATT 
TTGGCAAAGA CCAAATACTT TCAAATGTTG AAGATGCATG CGATGAATAC CAACAATATC 
ACTGAGCTAG TGAACTATTT GGCAAATGAC TTAAGTTTAG ATGAAGCTTC AGTCTTGATA 
ACTGAATATT CAAAGCACTG CGGGAAACCT GTGCCTCCAG ACACTGCTCC CTGTGAAATT 
CTGAAGATGT TTCTTAGTGG ATTATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055523.1
CDS	180..6809
Translation	MWNDIELLTNDDTGSGYLSVGSRKEHGTALYQVDLLVKISSEKASLNPKIQACSLSDGFIIVADQSVILLDSICRSLQLHLVFDTEVDVVGLCQEGKFLLVGERSGNLHLIHVTSKQTLLTNAFVQKANDENRRTYQNLVIEKDGSNEGTYYMLLLTYSGFFCITNLQLLKIQQAIENVDFSTAKKLQGQIKSSFISTENYHTLGCLSLVAGDLASEVPVIIGGTGNCAFSKWEPDSSKKGMTVKNLIDAEIIKGAKKFQLIDNLLFVLDTDNVLSLWDIYTLTPVWNWPSLHVEEFLLTTEADSPSSVTWQGITNLKLIALTASANKKMKNLMVYSLPTMEILYSLEVSSVSSLVQTGISTDTIYLLEGVCKNDPKLSEDSVSVLVLRCLTEALPENRLSRLLHKHRFAEAESFAIQFGLDVELVYKVKSNHILEKLALSSVDASEQTEWQQLVDDAKENLHKIQDDEFVVNYCLKAQWITYETTQEMLNYAKTRLLKKEDKTALIYSDGLKEVLRAHAKLTTFYGAFGPEKFSGSSWIEFLNNEDDLKDIFLQLKEGNLVCAQYLWLRHRANFESRFDVKMLESLLNSMSASVSLQKLCPWFKNDVIPFVRRTVPEGQIILAKWLEQAARNLELTDKANWPENGLQLAEIFFTAEKTDELGLASSWHWISLKDYQNTEEVCQLRTLVNNLRELITLHRKYNCKLALSDFEKENTTTIVFRMFDKVLAPELIPSILEKFIRVYMREHDLQEEELLLLYIEDLLNRCSSKSTSLFETAWEAKAMAVIACLSDTDLIFDAVLKIMYAAVVPWSAAVEQLVKQHLEMDHPKVKLLQESYKLMEMKKLLRGYGIREVNLLNKEIMRVVRYILKQDVPSSLEDALKVAQAFMLSDDEIYSLRIIDLIDREQGEDCLLLLKSLPPAEAEKTAERVIIWARLALQEEPDHSKEGKAWRMSVAKTSVDILKILCDIQKDNLQKKDECEEMLKLFKEVASLQENFEVFLSFEDYSNSSLVADLREQHIKAHEVAQAKHKPGSTPEPIAAEVRSPSMESKLHRQALALQMSKQELEAELTLRALKDGNIKTALKKCSDLFKYHCNADTGKLLFLTCQKLCQMLADNVPVTVPVGLNLPSMIHDLASQAATICSPDFLLDALELCKHTLMAVELSRQCQMDDCGILMKASFGTHKDPYEEWSYSDFFSEDGIVLESQMVLPVIYELISSLVPLAESKRYPLESTSLPYCSLNEGDGLVLPVINSISALLQNLQESSQWELALRFVVGSFGTCLQHSVSNFMNATLSEKLFGETTLVKSRHVVMELKEKAVIFIRENATTLLHKVFNCRLVDLDLALGYCTLLPQKDVFENLWKLIDKAWQNYDKILAISLVGSELASLYQEIEMGLKFRELSTDAQWGIRLGKLGISFQPVFRQHFLTKKDLIKALVENIDMDTSLILEYCSTFQLDCDAVLQLFIETLLHNTNAGQGQGDASMDSAKRRHPKLLAKALEMVPLLTSTKDLVISLSGILHKLDPYDYEMIEVVLKVIERADEKITNININQALSILKHLKSYRRISPPVDLEYQYMLEHVITLPSAAQTRLPFHLIFFGTAQNFWKILSTELSEESFPTLLLISKLMKFSLDTLYVSTAKHVFEKKLKPKLLKLTQAKSSTLINKEITKITQTIESCLLSIVNPEWAVAIAISLAQDIPEGSFKISALKFCLYLAERWLQNIPSQDEKREKAEALLKKLHIQYRRSGTEAVLIAHKLNTEEYLRVIGKPAHLIVSLYEHPSINQRIQNSSGTDYPDIHAAAKEIAEVNEINLEKVWDMLLEKWLCPSTKPGEKPSELFELQEDEALRRVQYLLLSRPIDYSSRMLFVFATSTTTTLGMHQLTFAHRTRALQCLFYLADKETIESLFKKPIEEVKSYLRCITFLASFETLNIPITYELFCSSPKEGMIKGLWKNHSHESMAVRLVTELCLEYKIYDLQLWNGLLQKLLGFNMIPYLRKVLKAISSIHSLWQVPYFSKAWQRVIQIPLLSASCPLSPDQLSDCSESLIAVLECPVSGDLDLIGVARQYIQLELPAFALACLMLMPHSEKRHQQIKNFLGSCDPQVILKQLEEHMNTGQLAGFSHQIRSLILNNIINKKEFGILAKTKYFQMLKMHAMNTNNITELVNYLANDLSLDEASVLITEYSKHCGKPVPPDTAPCEILKMFLSGLS

Target ORF information:

RefSeq Version	NM_014708.5
Organism	Homo sapiens(human)
Definition	Homo sapiens kinetochore associated 1 (KNTC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014708.5

ORF Insert Sequence:

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAACCA 
GTTTTCAGGC AACATTTTCT CACCAAGAAA GACCTCATTA AAGCTCTTGT GGAGAATATA 
GATATGGACA CAAGCCTCAT TTTGGAATAT TGCAGCACAT TTCAGTTGGA CTGCGATGCA 
GTTCTTCAGC TCTTCATTGA AACGCTGCTC CACAACACAA ATGCCGGCCA AGGCCAGGGA 
GATGCAAGCA TGGACTCTGC AAAGCGGCGG CATCCCAAAC TCCTGGCCAA AGCCCTTGAG 
ATGGTTCCTT TACTGACGAG CACAAAAGAT TTGGTCATCA GTCTTAGTGG AATACTACAT 
AAGTTGGATC CTTATGACTA TGAAATGATT GAAGTTGTCT TGAAAGTTAT AGAACGAGCT 
GATGAAAAGA TAACCAATAT TAATATTAAT CAGGCATTGA GTATTCTGAA ACATTTGAAG 
TCATACAGAA GAATTTCTCC TCCCGTGGAT CTAGAATATC AGTATATGTT GGAACATGTC 
ATAACTTTGC CATCAGCTGC CCAAACTAGA CTGCCTTTTC ACCTGATATT CTTTGGCACA 
GCACAGAACT TCTGGAAAAT TCTCTCTACA GAACTCAGTG AAGAATCTTT CCCAACATTG 
CTCTTAATTT CGAAATTAAT GAAGTTCTCT CTGGACACTC TGTACGTGTC TACAGCAAAA 
CACGTTTTCG AAAAAAAACT GAAGCCAAAG CTCCTGAAGT TAACACAAGC TAAATCCTCA 
ACACTGATTA ACAAGGAAAT AACTAAGATC ACGCAGACCA TCGAATCCTG CTTACTCTCT 
ATAGTCAACC CAGAGTGGGC TGTAGCTATT GCCATCAGCC TTGCCCAGGA TATCCCTGAA 
GGTTCCTTCA AGATATCTGC TTTGAAATTC TGCCTTTATT TAGCTGAGAG ATGGCTACAG 
AATATCCCAT CGCAGGACGA AAAACGTGAA AAAGCCGAGG CTTTGTTGAA GAAGCTTCAT 
ATCCAGTACC GGCGATCGGG CACAGAAGCT GTGCTCATAG CCCACAAGCT GAACACTGAG 
GAATATTTAA GAGTGATCGG AAAGCCAGCA CATCTTATTG TCAGTCTCTA CGAACATCCT 
AGCATCAATC AAAGAATTCA GAATTCATCT GGCACAGATT ATCCTGATAT TCATGCAGCA 
GCTAAAGAAA TAGCCGAAGT CAATGAAATT AATTTGGAAA AAGTCTGGGA CATGTTGTTG 
GAAAAATGGC TATGCCCTTC AACAAAACCT GGTGAAAAAC CATCAGAATT ATTTGAACTT 
CAAGAAGATG AAGCCCTACG AAGAGTGCAG TATCTCCTCC TGTCTCGTCC AATTGATTAT 
AGTTCAAGAA TGCTGTTTGT ATTTGCAACA TCAACTACAA CCACATTAGG TATGCATCAG 
TTAACTTTTG CCCATAGAAC TCGAGCTCTT CAGTGTCTCT TCTATTTGGC TGACAAGGAA 
ACTATAGAAT CTCTCTTTAA AAAACCCATT GAAGAAGTGA AATCTTATTT GAGATGTATA 
ACTTTTCTGG CATCATTTGA GACTTTGAAT ATCCCCATCA CATATGAATT ATTTTGCAGC 
AGTCCTAAAG AAGGAATGAT TAAGGGTCTG TGGAAAAACC ACAGCCACGA GTCCATGGCA 
GTAAGATTGG TGACTGAGCT GTGTTTAGAA TACAAAATCT ATGACCTGCA GCTTTGGAAT 
GGACTCTTGC AAAAGCTTCT GGGCTTCAAT ATGATTCCTT ATCTAAGGAA AGTTTTAAAA 
GCCATCTCCA GTATCCATTC TTTATGGCAG GTTCCCTACT TCAGCAAAGC GTGGCAGCGT 
GTGATACAGA TACCACTGCT TTCAGCCTCT TGTCCTTTAA GTCCTGATCA GCTGTCAGAT 
TGTTCTGAGA GTCTCATCGC TGTCCTCGAA TGTCCAGTCT CAGGTGATCT TGACCTGATC 
GGAGTCGCCA GGCAGTATAT CCAGTTAGAA CTTCCGGCTT TTGCATTAGC TTGTCTGATG 
CTCATGCCCC ACTCAGAGAA AAGACACCAG CAAATTAAGA ATTTTCTGGG TTCCTGTGAC 
CCTCAGGTTA TTTTAAAGCA ATTGGAAGAG CATATGAACA CGGGCCAGCT AGCAGGATTT 
TCACATCAAA TTAGAAGTCT GATTTTGAAT AATATCATCA ATAAGAAGGA GTTTGGGATT 
TTGGCAAAGA CCAAATACTT TCAAATGTTG AAGATGCATG CGATGAATAC CAACAATATC 
ACTGAGCTAG TGAACTATTT GGCAAATGAC TTAAGTTTAG ATGAAGCTTC AGTCTTGATA 
ACTGAATATT CAAAGCACTG CGGGAAACCT GTGCCTCCAG ACACTGCTCC CTGTGAAATT 
CTGAAGATGT TTCTTAGTGG ATTATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNTC1 ( NM_014708.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055523.1 Homo sapiens kinetochore associated 1 (KNTC1), mRNA.

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CloneID	OHu10474
Clone ID Related Accession (Same CDS sequence)	NM_014708.6 , NM_014708.5 , NM_014708.4 , XM_006719706.2
Accession Version	NM_014708.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-21
Organism	Homo sapiens(Human)
Product	kinetochore-associated protein 1
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC127002.3 and AC026333.28. This sequence is a reference standard in the RefSeqGene project. On Aug 16, 2007 this sequence version replaced NM_014708.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC150278.1, D79988.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAACCA 
GTTTTCAGGC AACATTTTCT CACCAAGAAA GACCTCATTA AAGCTCTTGT GGAGAATATA 
GATATGGACA CAAGCCTCAT TTTGGAATAT TGCAGCACAT TTCAGTTGGA CTGCGATGCA 
GTTCTTCAGC TCTTCATTGA AACGCTGCTC CACAACACAA ATGCCGGCCA AGGCCAGGGA 
GATGCAAGCA TGGACTCTGC AAAGCGGCGG CATCCCAAAC TCCTGGCCAA AGCCCTTGAG 
ATGGTTCCTT TACTGACGAG CACAAAAGAT TTGGTCATCA GTCTTAGTGG AATACTACAT 
AAGTTGGATC CTTATGACTA TGAAATGATT GAAGTTGTCT TGAAAGTTAT AGAACGAGCT 
GATGAAAAGA TAACCAATAT TAATATTAAT CAGGCATTGA GTATTCTGAA ACATTTGAAG 
TCATACAGAA GAATTTCTCC TCCCGTGGAT CTAGAATATC AGTATATGTT GGAACATGTC 
ATAACTTTGC CATCAGCTGC CCAAACTAGA CTGCCTTTTC ACCTGATATT CTTTGGCACA 
GCACAGAACT TCTGGAAAAT TCTCTCTACA GAACTCAGTG AAGAATCTTT CCCAACATTG 
CTCTTAATTT CGAAATTAAT GAAGTTCTCT CTGGACACTC TGTACGTGTC TACAGCAAAA 
CACGTTTTCG AAAAAAAACT GAAGCCAAAG CTCCTGAAGT TAACACAAGC TAAATCCTCA 
ACACTGATTA ACAAGGAAAT AACTAAGATC ACGCAGACCA TCGAATCCTG CTTACTCTCT 
ATAGTCAACC CAGAGTGGGC TGTAGCTATT GCCATCAGCC TTGCCCAGGA TATCCCTGAA 
GGTTCCTTCA AGATATCTGC TTTGAAATTC TGCCTTTATT TAGCTGAGAG ATGGCTACAG 
AATATCCCAT CGCAGGACGA AAAACGTGAA AAAGCCGAGG CTTTGTTGAA GAAGCTTCAT 
ATCCAGTACC GGCGATCGGG CACAGAAGCT GTGCTCATAG CCCACAAGCT GAACACTGAG 
GAATATTTAA GAGTGATCGG AAAGCCAGCA CATCTTATTG TCAGTCTCTA CGAACATCCT 
AGCATCAATC AAAGAATTCA GAATTCATCT GGCACAGATT ATCCTGATAT TCATGCAGCA 
GCTAAAGAAA TAGCCGAAGT CAATGAAATT AATTTGGAAA AAGTCTGGGA CATGTTGTTG 
GAAAAATGGC TATGCCCTTC AACAAAACCT GGTGAAAAAC CATCAGAATT ATTTGAACTT 
CAAGAAGATG AAGCCCTACG AAGAGTGCAG TATCTCCTCC TGTCTCGTCC AATTGATTAT 
AGTTCAAGAA TGCTGTTTGT ATTTGCAACA TCAACTACAA CCACATTAGG TATGCATCAG 
TTAACTTTTG CCCATAGAAC TCGAGCTCTT CAGTGTCTCT TCTATTTGGC TGACAAGGAA 
ACTATAGAAT CTCTCTTTAA AAAACCCATT GAAGAAGTGA AATCTTATTT GAGATGTATA 
ACTTTTCTGG CATCATTTGA GACTTTGAAT ATCCCCATCA CATATGAATT ATTTTGCAGC 
AGTCCTAAAG AAGGAATGAT TAAGGGTCTG TGGAAAAACC ACAGCCACGA GTCCATGGCA 
GTAAGATTGG TGACTGAGCT GTGTTTAGAA TACAAAATCT ATGACCTGCA GCTTTGGAAT 
GGACTCTTGC AAAAGCTTCT GGGCTTCAAT ATGATTCCTT ATCTAAGGAA AGTTTTAAAA 
GCCATCTCCA GTATCCATTC TTTATGGCAG GTTCCCTACT TCAGCAAAGC GTGGCAGCGT 
GTGATACAGA TACCACTGCT TTCAGCCTCT TGTCCTTTAA GTCCTGATCA GCTGTCAGAT 
TGTTCTGAGA GTCTCATCGC TGTCCTCGAA TGTCCAGTCT CAGGTGATCT TGACCTGATC 
GGAGTCGCCA GGCAGTATAT CCAGTTAGAA CTTCCGGCTT TTGCATTAGC TTGTCTGATG 
CTCATGCCCC ACTCAGAGAA AAGACACCAG CAAATTAAGA ATTTTCTGGG TTCCTGTGAC 
CCTCAGGTTA TTTTAAAGCA ATTGGAAGAG CATATGAACA CGGGCCAGCT AGCAGGATTT 
TCACATCAAA TTAGAAGTCT GATTTTGAAT AATATCATCA ATAAGAAGGA GTTTGGGATT 
TTGGCAAAGA CCAAATACTT TCAAATGTTG AAGATGCATG CGATGAATAC CAACAATATC 
ACTGAGCTAG TGAACTATTT GGCAAATGAC TTAAGTTTAG ATGAAGCTTC AGTCTTGATA 
ACTGAATATT CAAAGCACTG CGGGAAACCT GTGCCTCCAG ACACTGCTCC CTGTGAAATT 
CTGAAGATGT TTCTTAGTGG ATTATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055523.1
CDS	164..6793
Translation	MWNDIELLTNDDTGSGYLSVGSRKEHGTALYQVDLLVKISSEKASLNPKIQACSLSDGFIIVADQSVILLDSICRSLQLHLVFDTEVDVVGLCQEGKFLLVGERSGNLHLIHVTSKQTLLTNAFVQKANDENRRTYQNLVIEKDGSNEGTYYMLLLTYSGFFCITNLQLLKIQQAIENVDFSTAKKLQGQIKSSFISTENYHTLGCLSLVAGDLASEVPVIIGGTGNCAFSKWEPDSSKKGMTVKNLIDAEIIKGAKKFQLIDNLLFVLDTDNVLSLWDIYTLTPVWNWPSLHVEEFLLTTEADSPSSVTWQGITNLKLIALTASANKKMKNLMVYSLPTMEILYSLEVSSVSSLVQTGISTDTIYLLEGVCKNDPKLSEDSVSVLVLRCLTEALPENRLSRLLHKHRFAEAESFAIQFGLDVELVYKVKSNHILEKLALSSVDASEQTEWQQLVDDAKENLHKIQDDEFVVNYCLKAQWITYETTQEMLNYAKTRLLKKEDKTALIYSDGLKEVLRAHAKLTTFYGAFGPEKFSGSSWIEFLNNEDDLKDIFLQLKEGNLVCAQYLWLRHRANFESRFDVKMLESLLNSMSASVSLQKLCPWFKNDVIPFVRRTVPEGQIILAKWLEQAARNLELTDKANWPENGLQLAEIFFTAEKTDELGLASSWHWISLKDYQNTEEVCQLRTLVNNLRELITLHRKYNCKLALSDFEKENTTTIVFRMFDKVLAPELIPSILEKFIRVYMREHDLQEEELLLLYIEDLLNRCSSKSTSLFETAWEAKAMAVIACLSDTDLIFDAVLKIMYAAVVPWSAAVEQLVKQHLEMDHPKVKLLQESYKLMEMKKLLRGYGIREVNLLNKEIMRVVRYILKQDVPSSLEDALKVAQAFMLSDDEIYSLRIIDLIDREQGEDCLLLLKSLPPAEAEKTAERVIIWARLALQEEPDHSKEGKAWRMSVAKTSVDILKILCDIQKDNLQKKDECEEMLKLFKEVASLQENFEVFLSFEDYSNSSLVADLREQHIKAHEVAQAKHKPGSTPEPIAAEVRSPSMESKLHRQALALQMSKQELEAELTLRALKDGNIKTALKKCSDLFKYHCNADTGKLLFLTCQKLCQMLADNVPVTVPVGLNLPSMIHDLASQAATICSPDFLLDALELCKHTLMAVELSRQCQMDDCGILMKASFGTHKDPYEEWSYSDFFSEDGIVLESQMVLPVIYELISSLVPLAESKRYPLESTSLPYCSLNEGDGLVLPVINSISALLQNLQESSQWELALRFVVGSFGTCLQHSVSNFMNATLSEKLFGETTLVKSRHVVMELKEKAVIFIRENATTLLHKVFNCRLVDLDLALGYCTLLPQKDVFENLWKLIDKAWQNYDKILAISLVGSELASLYQEIEMGLKFRELSTDAQWGIRLGKLGISFQPVFRQHFLTKKDLIKALVENIDMDTSLILEYCSTFQLDCDAVLQLFIETLLHNTNAGQGQGDASMDSAKRRHPKLLAKALEMVPLLTSTKDLVISLSGILHKLDPYDYEMIEVVLKVIERADEKITNININQALSILKHLKSYRRISPPVDLEYQYMLEHVITLPSAAQTRLPFHLIFFGTAQNFWKILSTELSEESFPTLLLISKLMKFSLDTLYVSTAKHVFEKKLKPKLLKLTQAKSSTLINKEITKITQTIESCLLSIVNPEWAVAIAISLAQDIPEGSFKISALKFCLYLAERWLQNIPSQDEKREKAEALLKKLHIQYRRSGTEAVLIAHKLNTEEYLRVIGKPAHLIVSLYEHPSINQRIQNSSGTDYPDIHAAAKEIAEVNEINLEKVWDMLLEKWLCPSTKPGEKPSELFELQEDEALRRVQYLLLSRPIDYSSRMLFVFATSTTTTLGMHQLTFAHRTRALQCLFYLADKETIESLFKKPIEEVKSYLRCITFLASFETLNIPITYELFCSSPKEGMIKGLWKNHSHESMAVRLVTELCLEYKIYDLQLWNGLLQKLLGFNMIPYLRKVLKAISSIHSLWQVPYFSKAWQRVIQIPLLSASCPLSPDQLSDCSESLIAVLECPVSGDLDLIGVARQYIQLELPAFALACLMLMPHSEKRHQQIKNFLGSCDPQVILKQLEEHMNTGQLAGFSHQIRSLILNNIINKKEFGILAKTKYFQMLKMHAMNTNNITELVNYLANDLSLDEASVLITEYSKHCGKPVPPDTAPCEILKMFLSGLS

Target ORF information:

RefSeq Version	NM_014708.4
Organism	Homo sapiens(Human)
Definition	Homo sapiens kinetochore associated 1 (KNTC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014708.4

ORF Insert Sequence:

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAACCA 
GTTTTCAGGC AACATTTTCT CACCAAGAAA GACCTCATTA AAGCTCTTGT GGAGAATATA 
GATATGGACA CAAGCCTCAT TTTGGAATAT TGCAGCACAT TTCAGTTGGA CTGCGATGCA 
GTTCTTCAGC TCTTCATTGA AACGCTGCTC CACAACACAA ATGCCGGCCA AGGCCAGGGA 
GATGCAAGCA TGGACTCTGC AAAGCGGCGG CATCCCAAAC TCCTGGCCAA AGCCCTTGAG 
ATGGTTCCTT TACTGACGAG CACAAAAGAT TTGGTCATCA GTCTTAGTGG AATACTACAT 
AAGTTGGATC CTTATGACTA TGAAATGATT GAAGTTGTCT TGAAAGTTAT AGAACGAGCT 
GATGAAAAGA TAACCAATAT TAATATTAAT CAGGCATTGA GTATTCTGAA ACATTTGAAG 
TCATACAGAA GAATTTCTCC TCCCGTGGAT CTAGAATATC AGTATATGTT GGAACATGTC 
ATAACTTTGC CATCAGCTGC CCAAACTAGA CTGCCTTTTC ACCTGATATT CTTTGGCACA 
GCACAGAACT TCTGGAAAAT TCTCTCTACA GAACTCAGTG AAGAATCTTT CCCAACATTG 
CTCTTAATTT CGAAATTAAT GAAGTTCTCT CTGGACACTC TGTACGTGTC TACAGCAAAA 
CACGTTTTCG AAAAAAAACT GAAGCCAAAG CTCCTGAAGT TAACACAAGC TAAATCCTCA 
ACACTGATTA ACAAGGAAAT AACTAAGATC ACGCAGACCA TCGAATCCTG CTTACTCTCT 
ATAGTCAACC CAGAGTGGGC TGTAGCTATT GCCATCAGCC TTGCCCAGGA TATCCCTGAA 
GGTTCCTTCA AGATATCTGC TTTGAAATTC TGCCTTTATT TAGCTGAGAG ATGGCTACAG 
AATATCCCAT CGCAGGACGA AAAACGTGAA AAAGCCGAGG CTTTGTTGAA GAAGCTTCAT 
ATCCAGTACC GGCGATCGGG CACAGAAGCT GTGCTCATAG CCCACAAGCT GAACACTGAG 
GAATATTTAA GAGTGATCGG AAAGCCAGCA CATCTTATTG TCAGTCTCTA CGAACATCCT 
AGCATCAATC AAAGAATTCA GAATTCATCT GGCACAGATT ATCCTGATAT TCATGCAGCA 
GCTAAAGAAA TAGCCGAAGT CAATGAAATT AATTTGGAAA AAGTCTGGGA CATGTTGTTG 
GAAAAATGGC TATGCCCTTC AACAAAACCT GGTGAAAAAC CATCAGAATT ATTTGAACTT 
CAAGAAGATG AAGCCCTACG AAGAGTGCAG TATCTCCTCC TGTCTCGTCC AATTGATTAT 
AGTTCAAGAA TGCTGTTTGT ATTTGCAACA TCAACTACAA CCACATTAGG TATGCATCAG 
TTAACTTTTG CCCATAGAAC TCGAGCTCTT CAGTGTCTCT TCTATTTGGC TGACAAGGAA 
ACTATAGAAT CTCTCTTTAA AAAACCCATT GAAGAAGTGA AATCTTATTT GAGATGTATA 
ACTTTTCTGG CATCATTTGA GACTTTGAAT ATCCCCATCA CATATGAATT ATTTTGCAGC 
AGTCCTAAAG AAGGAATGAT TAAGGGTCTG TGGAAAAACC ACAGCCACGA GTCCATGGCA 
GTAAGATTGG TGACTGAGCT GTGTTTAGAA TACAAAATCT ATGACCTGCA GCTTTGGAAT 
GGACTCTTGC AAAAGCTTCT GGGCTTCAAT ATGATTCCTT ATCTAAGGAA AGTTTTAAAA 
GCCATCTCCA GTATCCATTC TTTATGGCAG GTTCCCTACT TCAGCAAAGC GTGGCAGCGT 
GTGATACAGA TACCACTGCT TTCAGCCTCT TGTCCTTTAA GTCCTGATCA GCTGTCAGAT 
TGTTCTGAGA GTCTCATCGC TGTCCTCGAA TGTCCAGTCT CAGGTGATCT TGACCTGATC 
GGAGTCGCCA GGCAGTATAT CCAGTTAGAA CTTCCGGCTT TTGCATTAGC TTGTCTGATG 
CTCATGCCCC ACTCAGAGAA AAGACACCAG CAAATTAAGA ATTTTCTGGG TTCCTGTGAC 
CCTCAGGTTA TTTTAAAGCA ATTGGAAGAG CATATGAACA CGGGCCAGCT AGCAGGATTT 
TCACATCAAA TTAGAAGTCT GATTTTGAAT AATATCATCA ATAAGAAGGA GTTTGGGATT 
TTGGCAAAGA CCAAATACTT TCAAATGTTG AAGATGCATG CGATGAATAC CAACAATATC 
ACTGAGCTAG TGAACTATTT GGCAAATGAC TTAAGTTTAG ATGAAGCTTC AGTCTTGATA 
ACTGAATATT CAAAGCACTG CGGGAAACCT GTGCCTCCAG ACACTGCTCC CTGTGAAATT 
CTGAAGATGT TTCTTAGTGG ATTATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNTC1 ( XM_006719706.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719769.1 Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X1, mRNA.

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CloneID	OHu10474
Clone ID Related Accession (Same CDS sequence)	NM_014708.6 , NM_014708.5 , NM_014708.4 , XM_006719706.2
Accession Version	XM_006719706.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	kinetochore-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006719706.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAACCA 
GTTTTCAGGC AACATTTTCT CACCAAGAAA GACCTCATTA AAGCTCTTGT GGAGAATATA 
GATATGGACA CAAGCCTCAT TTTGGAATAT TGCAGCACAT TTCAGTTGGA CTGCGATGCA 
GTTCTTCAGC TCTTCATTGA AACGCTGCTC CACAACACAA ATGCCGGCCA AGGCCAGGGA 
GATGCAAGCA TGGACTCTGC AAAGCGGCGG CATCCCAAAC TCCTGGCCAA AGCCCTTGAG 
ATGGTTCCTT TACTGACGAG CACAAAAGAT TTGGTCATCA GTCTTAGTGG AATACTACAT 
AAGTTGGATC CTTATGACTA TGAAATGATT GAAGTTGTCT TGAAAGTTAT AGAACGAGCT 
GATGAAAAGA TAACCAATAT TAATATTAAT CAGGCATTGA GTATTCTGAA ACATTTGAAG 
TCATACAGAA GAATTTCTCC TCCCGTGGAT CTAGAATATC AGTATATGTT GGAACATGTC 
ATAACTTTGC CATCAGCTGC CCAAACTAGA CTGCCTTTTC ACCTGATATT CTTTGGCACA 
GCACAGAACT TCTGGAAAAT TCTCTCTACA GAACTCAGTG AAGAATCTTT CCCAACATTG 
CTCTTAATTT CGAAATTAAT GAAGTTCTCT CTGGACACTC TGTACGTGTC TACAGCAAAA 
CACGTTTTCG AAAAAAAACT GAAGCCAAAG CTCCTGAAGT TAACACAAGC TAAATCCTCA 
ACACTGATTA ACAAGGAAAT AACTAAGATC ACGCAGACCA TCGAATCCTG CTTACTCTCT 
ATAGTCAACC CAGAGTGGGC TGTAGCTATT GCCATCAGCC TTGCCCAGGA TATCCCTGAA 
GGTTCCTTCA AGATATCTGC TTTGAAATTC TGCCTTTATT TAGCTGAGAG ATGGCTACAG 
AATATCCCAT CGCAGGACGA AAAACGTGAA AAAGCCGAGG CTTTGTTGAA GAAGCTTCAT 
ATCCAGTACC GGCGATCGGG CACAGAAGCT GTGCTCATAG CCCACAAGCT GAACACTGAG 
GAATATTTAA GAGTGATCGG AAAGCCAGCA CATCTTATTG TCAGTCTCTA CGAACATCCT 
AGCATCAATC AAAGAATTCA GAATTCATCT GGCACAGATT ATCCTGATAT TCATGCAGCA 
GCTAAAGAAA TAGCCGAAGT CAATGAAATT AATTTGGAAA AAGTCTGGGA CATGTTGTTG 
GAAAAATGGC TATGCCCTTC AACAAAACCT GGTGAAAAAC CATCAGAATT ATTTGAACTT 
CAAGAAGATG AAGCCCTACG AAGAGTGCAG TATCTCCTCC TGTCTCGTCC AATTGATTAT 
AGTTCAAGAA TGCTGTTTGT ATTTGCAACA TCAACTACAA CCACATTAGG TATGCATCAG 
TTAACTTTTG CCCATAGAAC TCGAGCTCTT CAGTGTCTCT TCTATTTGGC TGACAAGGAA 
ACTATAGAAT CTCTCTTTAA AAAACCCATT GAAGAAGTGA AATCTTATTT GAGATGTATA 
ACTTTTCTGG CATCATTTGA GACTTTGAAT ATCCCCATCA CATATGAATT ATTTTGCAGC 
AGTCCTAAAG AAGGAATGAT TAAGGGTCTG TGGAAAAACC ACAGCCACGA GTCCATGGCA 
GTAAGATTGG TGACTGAGCT GTGTTTAGAA TACAAAATCT ATGACCTGCA GCTTTGGAAT 
GGACTCTTGC AAAAGCTTCT GGGCTTCAAT ATGATTCCTT ATCTAAGGAA AGTTTTAAAA 
GCCATCTCCA GTATCCATTC TTTATGGCAG GTTCCCTACT TCAGCAAAGC GTGGCAGCGT 
GTGATACAGA TACCACTGCT TTCAGCCTCT TGTCCTTTAA GTCCTGATCA GCTGTCAGAT 
TGTTCTGAGA GTCTCATCGC TGTCCTCGAA TGTCCAGTCT CAGGTGATCT TGACCTGATC 
GGAGTCGCCA GGCAGTATAT CCAGTTAGAA CTTCCGGCTT TTGCATTAGC TTGTCTGATG 
CTCATGCCCC ACTCAGAGAA AAGACACCAG CAAATTAAGA ATTTTCTGGG TTCCTGTGAC 
CCTCAGGTTA TTTTAAAGCA ATTGGAAGAG CATATGAACA CGGGCCAGCT AGCAGGATTT 
TCACATCAAA TTAGAAGTCT GATTTTGAAT AATATCATCA ATAAGAAGGA GTTTGGGATT 
TTGGCAAAGA CCAAATACTT TCAAATGTTG AAGATGCATG CGATGAATAC CAACAATATC 
ACTGAGCTAG TGAACTATTT GGCAAATGAC TTAAGTTTAG ATGAAGCTTC AGTCTTGATA 
ACTGAATATT CAAAGCACTG CGGGAAACCT GTGCCTCCAG ACACTGCTCC CTGTGAAATT 
CTGAAGATGT TTCTTAGTGG ATTATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719769.1
CDS	146..6775
Translation	MWNDIELLTNDDTGSGYLSVGSRKEHGTALYQVDLLVKISSEKASLNPKIQACSLSDGFIIVADQSVILLDSICRSLQLHLVFDTEVDVVGLCQEGKFLLVGERSGNLHLIHVTSKQTLLTNAFVQKANDENRRTYQNLVIEKDGSNEGTYYMLLLTYSGFFCITNLQLLKIQQAIENVDFSTAKKLQGQIKSSFISTENYHTLGCLSLVAGDLASEVPVIIGGTGNCAFSKWEPDSSKKGMTVKNLIDAEIIKGAKKFQLIDNLLFVLDTDNVLSLWDIYTLTPVWNWPSLHVEEFLLTTEADSPSSVTWQGITNLKLIALTASANKKMKNLMVYSLPTMEILYSLEVSSVSSLVQTGISTDTIYLLEGVCKNDPKLSEDSVSVLVLRCLTEALPENRLSRLLHKHRFAEAESFAIQFGLDVELVYKVKSNHILEKLALSSVDASEQTEWQQLVDDAKENLHKIQDDEFVVNYCLKAQWITYETTQEMLNYAKTRLLKKEDKTALIYSDGLKEVLRAHAKLTTFYGAFGPEKFSGSSWIEFLNNEDDLKDIFLQLKEGNLVCAQYLWLRHRANFESRFDVKMLESLLNSMSASVSLQKLCPWFKNDVIPFVRRTVPEGQIILAKWLEQAARNLELTDKANWPENGLQLAEIFFTAEKTDELGLASSWHWISLKDYQNTEEVCQLRTLVNNLRELITLHRKYNCKLALSDFEKENTTTIVFRMFDKVLAPELIPSILEKFIRVYMREHDLQEEELLLLYIEDLLNRCSSKSTSLFETAWEAKAMAVIACLSDTDLIFDAVLKIMYAAVVPWSAAVEQLVKQHLEMDHPKVKLLQESYKLMEMKKLLRGYGIREVNLLNKEIMRVVRYILKQDVPSSLEDALKVAQAFMLSDDEIYSLRIIDLIDREQGEDCLLLLKSLPPAEAEKTAERVIIWARLALQEEPDHSKEGKAWRMSVAKTSVDILKILCDIQKDNLQKKDECEEMLKLFKEVASLQENFEVFLSFEDYSNSSLVADLREQHIKAHEVAQAKHKPGSTPEPIAAEVRSPSMESKLHRQALALQMSKQELEAELTLRALKDGNIKTALKKCSDLFKYHCNADTGKLLFLTCQKLCQMLADNVPVTVPVGLNLPSMIHDLASQAATICSPDFLLDALELCKHTLMAVELSRQCQMDDCGILMKASFGTHKDPYEEWSYSDFFSEDGIVLESQMVLPVIYELISSLVPLAESKRYPLESTSLPYCSLNEGDGLVLPVINSISALLQNLQESSQWELALRFVVGSFGTCLQHSVSNFMNATLSEKLFGETTLVKSRHVVMELKEKAVIFIRENATTLLHKVFNCRLVDLDLALGYCTLLPQKDVFENLWKLIDKAWQNYDKILAISLVGSELASLYQEIEMGLKFRELSTDAQWGIRLGKLGISFQPVFRQHFLTKKDLIKALVENIDMDTSLILEYCSTFQLDCDAVLQLFIETLLHNTNAGQGQGDASMDSAKRRHPKLLAKALEMVPLLTSTKDLVISLSGILHKLDPYDYEMIEVVLKVIERADEKITNININQALSILKHLKSYRRISPPVDLEYQYMLEHVITLPSAAQTRLPFHLIFFGTAQNFWKILSTELSEESFPTLLLISKLMKFSLDTLYVSTAKHVFEKKLKPKLLKLTQAKSSTLINKEITKITQTIESCLLSIVNPEWAVAIAISLAQDIPEGSFKISALKFCLYLAERWLQNIPSQDEKREKAEALLKKLHIQYRRSGTEAVLIAHKLNTEEYLRVIGKPAHLIVSLYEHPSINQRIQNSSGTDYPDIHAAAKEIAEVNEINLEKVWDMLLEKWLCPSTKPGEKPSELFELQEDEALRRVQYLLLSRPIDYSSRMLFVFATSTTTTLGMHQLTFAHRTRALQCLFYLADKETIESLFKKPIEEVKSYLRCITFLASFETLNIPITYELFCSSPKEGMIKGLWKNHSHESMAVRLVTELCLEYKIYDLQLWNGLLQKLLGFNMIPYLRKVLKAISSIHSLWQVPYFSKAWQRVIQIPLLSASCPLSPDQLSDCSESLIAVLECPVSGDLDLIGVARQYIQLELPAFALACLMLMPHSEKRHQQIKNFLGSCDPQVILKQLEEHMNTGQLAGFSHQIRSLILNNIINKKEFGILAKTKYFQMLKMHAMNTNNITELVNYLANDLSLDEASVLITEYSKHCGKPVPPDTAPCEILKMFLSGLS

Target ORF information:

RefSeq Version	XM_006719706.2
Organism	Homo sapiens(human)
Definition	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006719706.2

ORF Insert Sequence:

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAACCA 
GTTTTCAGGC AACATTTTCT CACCAAGAAA GACCTCATTA AAGCTCTTGT GGAGAATATA 
GATATGGACA CAAGCCTCAT TTTGGAATAT TGCAGCACAT TTCAGTTGGA CTGCGATGCA 
GTTCTTCAGC TCTTCATTGA AACGCTGCTC CACAACACAA ATGCCGGCCA AGGCCAGGGA 
GATGCAAGCA TGGACTCTGC AAAGCGGCGG CATCCCAAAC TCCTGGCCAA AGCCCTTGAG 
ATGGTTCCTT TACTGACGAG CACAAAAGAT TTGGTCATCA GTCTTAGTGG AATACTACAT 
AAGTTGGATC CTTATGACTA TGAAATGATT GAAGTTGTCT TGAAAGTTAT AGAACGAGCT 
GATGAAAAGA TAACCAATAT TAATATTAAT CAGGCATTGA GTATTCTGAA ACATTTGAAG 
TCATACAGAA GAATTTCTCC TCCCGTGGAT CTAGAATATC AGTATATGTT GGAACATGTC 
ATAACTTTGC CATCAGCTGC CCAAACTAGA CTGCCTTTTC ACCTGATATT CTTTGGCACA 
GCACAGAACT TCTGGAAAAT TCTCTCTACA GAACTCAGTG AAGAATCTTT CCCAACATTG 
CTCTTAATTT CGAAATTAAT GAAGTTCTCT CTGGACACTC TGTACGTGTC TACAGCAAAA 
CACGTTTTCG AAAAAAAACT GAAGCCAAAG CTCCTGAAGT TAACACAAGC TAAATCCTCA 
ACACTGATTA ACAAGGAAAT AACTAAGATC ACGCAGACCA TCGAATCCTG CTTACTCTCT 
ATAGTCAACC CAGAGTGGGC TGTAGCTATT GCCATCAGCC TTGCCCAGGA TATCCCTGAA 
GGTTCCTTCA AGATATCTGC TTTGAAATTC TGCCTTTATT TAGCTGAGAG ATGGCTACAG 
AATATCCCAT CGCAGGACGA AAAACGTGAA AAAGCCGAGG CTTTGTTGAA GAAGCTTCAT 
ATCCAGTACC GGCGATCGGG CACAGAAGCT GTGCTCATAG CCCACAAGCT GAACACTGAG 
GAATATTTAA GAGTGATCGG AAAGCCAGCA CATCTTATTG TCAGTCTCTA CGAACATCCT 
AGCATCAATC AAAGAATTCA GAATTCATCT GGCACAGATT ATCCTGATAT TCATGCAGCA 
GCTAAAGAAA TAGCCGAAGT CAATGAAATT AATTTGGAAA AAGTCTGGGA CATGTTGTTG 
GAAAAATGGC TATGCCCTTC AACAAAACCT GGTGAAAAAC CATCAGAATT ATTTGAACTT 
CAAGAAGATG AAGCCCTACG AAGAGTGCAG TATCTCCTCC TGTCTCGTCC AATTGATTAT 
AGTTCAAGAA TGCTGTTTGT ATTTGCAACA TCAACTACAA CCACATTAGG TATGCATCAG 
TTAACTTTTG CCCATAGAAC TCGAGCTCTT CAGTGTCTCT TCTATTTGGC TGACAAGGAA 
ACTATAGAAT CTCTCTTTAA AAAACCCATT GAAGAAGTGA AATCTTATTT GAGATGTATA 
ACTTTTCTGG CATCATTTGA GACTTTGAAT ATCCCCATCA CATATGAATT ATTTTGCAGC 
AGTCCTAAAG AAGGAATGAT TAAGGGTCTG TGGAAAAACC ACAGCCACGA GTCCATGGCA 
GTAAGATTGG TGACTGAGCT GTGTTTAGAA TACAAAATCT ATGACCTGCA GCTTTGGAAT 
GGACTCTTGC AAAAGCTTCT GGGCTTCAAT ATGATTCCTT ATCTAAGGAA AGTTTTAAAA 
GCCATCTCCA GTATCCATTC TTTATGGCAG GTTCCCTACT TCAGCAAAGC GTGGCAGCGT 
GTGATACAGA TACCACTGCT TTCAGCCTCT TGTCCTTTAA GTCCTGATCA GCTGTCAGAT 
TGTTCTGAGA GTCTCATCGC TGTCCTCGAA TGTCCAGTCT CAGGTGATCT TGACCTGATC 
GGAGTCGCCA GGCAGTATAT CCAGTTAGAA CTTCCGGCTT TTGCATTAGC TTGTCTGATG 
CTCATGCCCC ACTCAGAGAA AAGACACCAG CAAATTAAGA ATTTTCTGGG TTCCTGTGAC 
CCTCAGGTTA TTTTAAAGCA ATTGGAAGAG CATATGAACA CGGGCCAGCT AGCAGGATTT 
TCACATCAAA TTAGAAGTCT GATTTTGAAT AATATCATCA ATAAGAAGGA GTTTGGGATT 
TTGGCAAAGA CCAAATACTT TCAAATGTTG AAGATGCATG CGATGAATAC CAACAATATC 
ACTGAGCTAG TGAACTATTT GGCAAATGAC TTAAGTTTAG ATGAAGCTTC AGTCTTGATA 
ACTGAATATT CAAAGCACTG CGGGAAACCT GTGCCTCCAG ACACTGCTCC CTGTGAAATT 
CTGAAGATGT TTCTTAGTGG ATTATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNTC1 ( XM_011539027.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537329.1 Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X2, mRNA.

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CloneID	OHu63241
Clone ID Related Accession (Same CDS sequence)	XM_011539027.3 , XM_011539027.2
Accession Version	XM_011539027.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	kinetochore-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011539027.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAACCA 
GTTTTCAGGC AACATTTTCT CACCAAGAAA GACCTCATTA AAGCTCTTGT GGAGAATATA 
GATATGGACA CAAGCCTCAT TTTGGAATAT TGCAGCACAT TTCAGTTGGA CTGCGATGCA 
GTTCTTCAGC TCTTCATTGA AACGCTGCTC CACAACACAA ATGCCGGCCA AGGCCAGGGA 
GATGCAAGCA TGGACTCTGC AAAGCGGCGG CATCCCAAAC TCCTGGCCAA AGCCCTTGAG 
ATGGTTCCTT TACTGACGAG CACAAAAGAT TTGGTCATCA GTCTTAGTGG AATACTACAT 
AAGTTGGATC CTTATGACTA TGAAATGATT GAAGTTGTCT TGAAAGTTAT AGAACGAGCT 
GATGAAAAGA TAACCAATAT TAATATTAAT CAGGCATTGA GTATTCTGAA ACATTTGAAG 
TCATACAGAA GAATTTCTCC TCCCGTGGAT CTAGAATATC AGTATATGTT GGAACATGTC 
ATAACTTTGC CATCAGCTGC CCAAACTAGA CTGCCTTTTC ACCTGATATT CTTTGGCACA 
GCACAGAACT TCTGGAAAAT TCTCTCTACA GAACTCAGTG AAGAATCTTT CCCAACATTG 
CTCTTAATTT CGAAATTAAT GAAGTTCTCT CTGGACACTC TGTACGTGTC TACAGCAAAA 
CACGTTTTCG AAAAAAAACT GAAGCCAAAG CTCCTGAAGT TAACACAAGC TAAATCCTCA 
ACACTGATTA ACAAGGAAAT AACTAAGATC ACGCAGACCA TCGAATCCTG CTTACTCTCT 
ATAGTCAACC CAGAGTGGGC TGTAGCTATT GCCATCAGCC TTGCCCAGGA TATCCCTGAA 
GGTTCCTTCA AGATATCTGC TTTGAAATTC TGCCTTTATT TAGCTGAGAG ATGGCTACAG 
AATATCCCAT CGCAGGACGA AAAACGTGAA AAAGCCGAGG CTTTGTTGAA GAAGCTTCAT 
ATCCAGTACC GGCGATCGGG CACAGAAGCT GTGCTCATAG CCCACAAGCT GAACACTGAG 
GAATATTTAA GAGTGATCGG AAAGCCAGCA CATCTTATTG TCAGTCTCTA CGAACATCCT 
AGCATCAATC AAAGAATTCA GAATTCATCT GGCACAGATT ATCCTGATAT TCATGCAGCA 
GCTAAAGAAA TAGCCGAAGT CAATGAAATT AATTTGGAAA AAGTCTGGGA CATGTTGTTG 
GAAAAATGGC TATGCCCTTC AACAAAACCT GGTGAAAAAC CATCAGAATT ATTTGAACTT 
CAAGAAGATG AAGCCCTACG AAGAGTGCAG TATCTCCTCC TGTCTCGTCC AATTGATTAT 
AGTTCAAGAA TGCTGTTTGT ATTTGCAACA TCAACTACAA CCACATTAGG TATGCATCAG 
TTAACTTTTG CCCATAGAAC TCGAGCTCTT CAGTGTCTCT TCTATTTGGC TGACAAGGAA 
ACTATAGAAT CTCTCTTTAA AAAACCCATT GAAGAAGTGA ACAGTCCTAA AGAAGGAATG 
ATTAAGGGTC TGTGGAAAAA CCACAGCCAC GAGTCCATGG CAGTAAGATT GGTGACTGAG 
CTGTGTTTAG AATACAAAAT CTATGACCTG CAGCTTTGGA ATGGACTCTT GCAAAAGCTT 
CTGGGCTTCA ATATGATTCC TTATCTAAGG AAAGTTTTAA AAGCCATCTC CAGTATCCAT 
TCTTTATGGC AGGTTCCCTA CTTCAGCAAA GCGTGGCAGC GTGTGATACA GATACCACTG 
CTTTCAGCCT CTTGTCCTTT AAGTCCTGAT CAGCTGTCAG ATTGTTCTGA GAGTCTCATC 
GCTGTCCTCG AATGTCCAGT CTCAGGTGAT CTTGACCTGA TCGGAGTCGC CAGGCAGTAT 
ATCCAGTTAG AACTTCCGGC TTTTGCATTA GCTTGTCTGA TGCTCATGCC CCACTCAGAG 
AAAAGACACC AGCAAATTAA GAATTTTCTG GGTTCCTGTG ACCCTCAGGT TATTTTAAAG 
CAATTGGAAG AGCATATGAA CACGGGCCAG CTAGCAGGAT TTTCACATCA AATTAGAAGT 
CTGATTTTGA ATAATATCAT CAATAAGAAG GAGTTTGGGA TTTTGGCAAA GACCAAATAC 
TTTCAAATGT TGAAGATGCA TGCGATGAAT ACCAACAATA TCACTGAGCT AGTGAACTAT 
TTGGCAAATG ACTTAAGTTT AGATGAAGCT TCAGTCTTGA TAACTGAATA TTCAAAGCAC 
TGCGGGAAAC CTGTGCCTCC AGACACTGCT CCCTGTGAAA TTCTGAAGAT GTTTCTTAGT 
GGATTATCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537329.1
CDS	146..6697
Translation	MWNDIELLTNDDTGSGYLSVGSRKEHGTALYQVDLLVKISSEKASLNPKIQACSLSDGFIIVADQSVILLDSICRSLQLHLVFDTEVDVVGLCQEGKFLLVGERSGNLHLIHVTSKQTLLTNAFVQKANDENRRTYQNLVIEKDGSNEGTYYMLLLTYSGFFCITNLQLLKIQQAIENVDFSTAKKLQGQIKSSFISTENYHTLGCLSLVAGDLASEVPVIIGGTGNCAFSKWEPDSSKKGMTVKNLIDAEIIKGAKKFQLIDNLLFVLDTDNVLSLWDIYTLTPVWNWPSLHVEEFLLTTEADSPSSVTWQGITNLKLIALTASANKKMKNLMVYSLPTMEILYSLEVSSVSSLVQTGISTDTIYLLEGVCKNDPKLSEDSVSVLVLRCLTEALPENRLSRLLHKHRFAEAESFAIQFGLDVELVYKVKSNHILEKLALSSVDASEQTEWQQLVDDAKENLHKIQDDEFVVNYCLKAQWITYETTQEMLNYAKTRLLKKEDKTALIYSDGLKEVLRAHAKLTTFYGAFGPEKFSGSSWIEFLNNEDDLKDIFLQLKEGNLVCAQYLWLRHRANFESRFDVKMLESLLNSMSASVSLQKLCPWFKNDVIPFVRRTVPEGQIILAKWLEQAARNLELTDKANWPENGLQLAEIFFTAEKTDELGLASSWHWISLKDYQNTEEVCQLRTLVNNLRELITLHRKYNCKLALSDFEKENTTTIVFRMFDKVLAPELIPSILEKFIRVYMREHDLQEEELLLLYIEDLLNRCSSKSTSLFETAWEAKAMAVIACLSDTDLIFDAVLKIMYAAVVPWSAAVEQLVKQHLEMDHPKVKLLQESYKLMEMKKLLRGYGIREVNLLNKEIMRVVRYILKQDVPSSLEDALKVAQAFMLSDDEIYSLRIIDLIDREQGEDCLLLLKSLPPAEAEKTAERVIIWARLALQEEPDHSKEGKAWRMSVAKTSVDILKILCDIQKDNLQKKDECEEMLKLFKEVASLQENFEVFLSFEDYSNSSLVADLREQHIKAHEVAQAKHKPGSTPEPIAAEVRSPSMESKLHRQALALQMSKQELEAELTLRALKDGNIKTALKKCSDLFKYHCNADTGKLLFLTCQKLCQMLADNVPVTVPVGLNLPSMIHDLASQAATICSPDFLLDALELCKHTLMAVELSRQCQMDDCGILMKASFGTHKDPYEEWSYSDFFSEDGIVLESQMVLPVIYELISSLVPLAESKRYPLESTSLPYCSLNEGDGLVLPVINSISALLQNLQESSQWELALRFVVGSFGTCLQHSVSNFMNATLSEKLFGETTLVKSRHVVMELKEKAVIFIRENATTLLHKVFNCRLVDLDLALGYCTLLPQKDVFENLWKLIDKAWQNYDKILAISLVGSELASLYQEIEMGLKFRELSTDAQWGIRLGKLGISFQPVFRQHFLTKKDLIKALVENIDMDTSLILEYCSTFQLDCDAVLQLFIETLLHNTNAGQGQGDASMDSAKRRHPKLLAKALEMVPLLTSTKDLVISLSGILHKLDPYDYEMIEVVLKVIERADEKITNININQALSILKHLKSYRRISPPVDLEYQYMLEHVITLPSAAQTRLPFHLIFFGTAQNFWKILSTELSEESFPTLLLISKLMKFSLDTLYVSTAKHVFEKKLKPKLLKLTQAKSSTLINKEITKITQTIESCLLSIVNPEWAVAIAISLAQDIPEGSFKISALKFCLYLAERWLQNIPSQDEKREKAEALLKKLHIQYRRSGTEAVLIAHKLNTEEYLRVIGKPAHLIVSLYEHPSINQRIQNSSGTDYPDIHAAAKEIAEVNEINLEKVWDMLLEKWLCPSTKPGEKPSELFELQEDEALRRVQYLLLSRPIDYSSRMLFVFATSTTTTLGMHQLTFAHRTRALQCLFYLADKETIESLFKKPIEEVNSPKEGMIKGLWKNHSHESMAVRLVTELCLEYKIYDLQLWNGLLQKLLGFNMIPYLRKVLKAISSIHSLWQVPYFSKAWQRVIQIPLLSASCPLSPDQLSDCSESLIAVLECPVSGDLDLIGVARQYIQLELPAFALACLMLMPHSEKRHQQIKNFLGSCDPQVILKQLEEHMNTGQLAGFSHQIRSLILNNIINKKEFGILAKTKYFQMLKMHAMNTNNITELVNYLANDLSLDEASVLITEYSKHCGKPVPPDTAPCEILKMFLSGLS

Target ORF information:

RefSeq Version	XM_011539027.3
Organism	Homo sapiens(human)
Definition	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539027.3

ORF Insert Sequence:

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAACCA 
GTTTTCAGGC AACATTTTCT CACCAAGAAA GACCTCATTA AAGCTCTTGT GGAGAATATA 
GATATGGACA CAAGCCTCAT TTTGGAATAT TGCAGCACAT TTCAGTTGGA CTGCGATGCA 
GTTCTTCAGC TCTTCATTGA AACGCTGCTC CACAACACAA ATGCCGGCCA AGGCCAGGGA 
GATGCAAGCA TGGACTCTGC AAAGCGGCGG CATCCCAAAC TCCTGGCCAA AGCCCTTGAG 
ATGGTTCCTT TACTGACGAG CACAAAAGAT TTGGTCATCA GTCTTAGTGG AATACTACAT 
AAGTTGGATC CTTATGACTA TGAAATGATT GAAGTTGTCT TGAAAGTTAT AGAACGAGCT 
GATGAAAAGA TAACCAATAT TAATATTAAT CAGGCATTGA GTATTCTGAA ACATTTGAAG 
TCATACAGAA GAATTTCTCC TCCCGTGGAT CTAGAATATC AGTATATGTT GGAACATGTC 
ATAACTTTGC CATCAGCTGC CCAAACTAGA CTGCCTTTTC ACCTGATATT CTTTGGCACA 
GCACAGAACT TCTGGAAAAT TCTCTCTACA GAACTCAGTG AAGAATCTTT CCCAACATTG 
CTCTTAATTT CGAAATTAAT GAAGTTCTCT CTGGACACTC TGTACGTGTC TACAGCAAAA 
CACGTTTTCG AAAAAAAACT GAAGCCAAAG CTCCTGAAGT TAACACAAGC TAAATCCTCA 
ACACTGATTA ACAAGGAAAT AACTAAGATC ACGCAGACCA TCGAATCCTG CTTACTCTCT 
ATAGTCAACC CAGAGTGGGC TGTAGCTATT GCCATCAGCC TTGCCCAGGA TATCCCTGAA 
GGTTCCTTCA AGATATCTGC TTTGAAATTC TGCCTTTATT TAGCTGAGAG ATGGCTACAG 
AATATCCCAT CGCAGGACGA AAAACGTGAA AAAGCCGAGG CTTTGTTGAA GAAGCTTCAT 
ATCCAGTACC GGCGATCGGG CACAGAAGCT GTGCTCATAG CCCACAAGCT GAACACTGAG 
GAATATTTAA GAGTGATCGG AAAGCCAGCA CATCTTATTG TCAGTCTCTA CGAACATCCT 
AGCATCAATC AAAGAATTCA GAATTCATCT GGCACAGATT ATCCTGATAT TCATGCAGCA 
GCTAAAGAAA TAGCCGAAGT CAATGAAATT AATTTGGAAA AAGTCTGGGA CATGTTGTTG 
GAAAAATGGC TATGCCCTTC AACAAAACCT GGTGAAAAAC CATCAGAATT ATTTGAACTT 
CAAGAAGATG AAGCCCTACG AAGAGTGCAG TATCTCCTCC TGTCTCGTCC AATTGATTAT 
AGTTCAAGAA TGCTGTTTGT ATTTGCAACA TCAACTACAA CCACATTAGG TATGCATCAG 
TTAACTTTTG CCCATAGAAC TCGAGCTCTT CAGTGTCTCT TCTATTTGGC TGACAAGGAA 
ACTATAGAAT CTCTCTTTAA AAAACCCATT GAAGAAGTGA ACAGTCCTAA AGAAGGAATG 
ATTAAGGGTC TGTGGAAAAA CCACAGCCAC GAGTCCATGG CAGTAAGATT GGTGACTGAG 
CTGTGTTTAG AATACAAAAT CTATGACCTG CAGCTTTGGA ATGGACTCTT GCAAAAGCTT 
CTGGGCTTCA ATATGATTCC TTATCTAAGG AAAGTTTTAA AAGCCATCTC CAGTATCCAT 
TCTTTATGGC AGGTTCCCTA CTTCAGCAAA GCGTGGCAGC GTGTGATACA GATACCACTG 
CTTTCAGCCT CTTGTCCTTT AAGTCCTGAT CAGCTGTCAG ATTGTTCTGA GAGTCTCATC 
GCTGTCCTCG AATGTCCAGT CTCAGGTGAT CTTGACCTGA TCGGAGTCGC CAGGCAGTAT 
ATCCAGTTAG AACTTCCGGC TTTTGCATTA GCTTGTCTGA TGCTCATGCC CCACTCAGAG 
AAAAGACACC AGCAAATTAA GAATTTTCTG GGTTCCTGTG ACCCTCAGGT TATTTTAAAG 
CAATTGGAAG AGCATATGAA CACGGGCCAG CTAGCAGGAT TTTCACATCA AATTAGAAGT 
CTGATTTTGA ATAATATCAT CAATAAGAAG GAGTTTGGGA TTTTGGCAAA GACCAAATAC 
TTTCAAATGT TGAAGATGCA TGCGATGAAT ACCAACAATA TCACTGAGCT AGTGAACTAT 
TTGGCAAATG ACTTAAGTTT AGATGAAGCT TCAGTCTTGA TAACTGAATA TTCAAAGCAC 
TGCGGGAAAC CTGTGCCTCC AGACACTGCT CCCTGTGAAA TTCTGAAGAT GTTTCTTAGT 
GGATTATCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KNTC1 ( XM_011539027.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537329.1 Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X2, mRNA.

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CloneID	OHu63241
Clone ID Related Accession (Same CDS sequence)	XM_011539027.3 , XM_011539027.2
Accession Version	XM_011539027.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	kinetochore-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011539027.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAACCA 
GTTTTCAGGC AACATTTTCT CACCAAGAAA GACCTCATTA AAGCTCTTGT GGAGAATATA 
GATATGGACA CAAGCCTCAT TTTGGAATAT TGCAGCACAT TTCAGTTGGA CTGCGATGCA 
GTTCTTCAGC TCTTCATTGA AACGCTGCTC CACAACACAA ATGCCGGCCA AGGCCAGGGA 
GATGCAAGCA TGGACTCTGC AAAGCGGCGG CATCCCAAAC TCCTGGCCAA AGCCCTTGAG 
ATGGTTCCTT TACTGACGAG CACAAAAGAT TTGGTCATCA GTCTTAGTGG AATACTACAT 
AAGTTGGATC CTTATGACTA TGAAATGATT GAAGTTGTCT TGAAAGTTAT AGAACGAGCT 
GATGAAAAGA TAACCAATAT TAATATTAAT CAGGCATTGA GTATTCTGAA ACATTTGAAG 
TCATACAGAA GAATTTCTCC TCCCGTGGAT CTAGAATATC AGTATATGTT GGAACATGTC 
ATAACTTTGC CATCAGCTGC CCAAACTAGA CTGCCTTTTC ACCTGATATT CTTTGGCACA 
GCACAGAACT TCTGGAAAAT TCTCTCTACA GAACTCAGTG AAGAATCTTT CCCAACATTG 
CTCTTAATTT CGAAATTAAT GAAGTTCTCT CTGGACACTC TGTACGTGTC TACAGCAAAA 
CACGTTTTCG AAAAAAAACT GAAGCCAAAG CTCCTGAAGT TAACACAAGC TAAATCCTCA 
ACACTGATTA ACAAGGAAAT AACTAAGATC ACGCAGACCA TCGAATCCTG CTTACTCTCT 
ATAGTCAACC CAGAGTGGGC TGTAGCTATT GCCATCAGCC TTGCCCAGGA TATCCCTGAA 
GGTTCCTTCA AGATATCTGC TTTGAAATTC TGCCTTTATT TAGCTGAGAG ATGGCTACAG 
AATATCCCAT CGCAGGACGA AAAACGTGAA AAAGCCGAGG CTTTGTTGAA GAAGCTTCAT 
ATCCAGTACC GGCGATCGGG CACAGAAGCT GTGCTCATAG CCCACAAGCT GAACACTGAG 
GAATATTTAA GAGTGATCGG AAAGCCAGCA CATCTTATTG TCAGTCTCTA CGAACATCCT 
AGCATCAATC AAAGAATTCA GAATTCATCT GGCACAGATT ATCCTGATAT TCATGCAGCA 
GCTAAAGAAA TAGCCGAAGT CAATGAAATT AATTTGGAAA AAGTCTGGGA CATGTTGTTG 
GAAAAATGGC TATGCCCTTC AACAAAACCT GGTGAAAAAC CATCAGAATT ATTTGAACTT 
CAAGAAGATG AAGCCCTACG AAGAGTGCAG TATCTCCTCC TGTCTCGTCC AATTGATTAT 
AGTTCAAGAA TGCTGTTTGT ATTTGCAACA TCAACTACAA CCACATTAGG TATGCATCAG 
TTAACTTTTG CCCATAGAAC TCGAGCTCTT CAGTGTCTCT TCTATTTGGC TGACAAGGAA 
ACTATAGAAT CTCTCTTTAA AAAACCCATT GAAGAAGTGA ACAGTCCTAA AGAAGGAATG 
ATTAAGGGTC TGTGGAAAAA CCACAGCCAC GAGTCCATGG CAGTAAGATT GGTGACTGAG 
CTGTGTTTAG AATACAAAAT CTATGACCTG CAGCTTTGGA ATGGACTCTT GCAAAAGCTT 
CTGGGCTTCA ATATGATTCC TTATCTAAGG AAAGTTTTAA AAGCCATCTC CAGTATCCAT 
TCTTTATGGC AGGTTCCCTA CTTCAGCAAA GCGTGGCAGC GTGTGATACA GATACCACTG 
CTTTCAGCCT CTTGTCCTTT AAGTCCTGAT CAGCTGTCAG ATTGTTCTGA GAGTCTCATC 
GCTGTCCTCG AATGTCCAGT CTCAGGTGAT CTTGACCTGA TCGGAGTCGC CAGGCAGTAT 
ATCCAGTTAG AACTTCCGGC TTTTGCATTA GCTTGTCTGA TGCTCATGCC CCACTCAGAG 
AAAAGACACC AGCAAATTAA GAATTTTCTG GGTTCCTGTG ACCCTCAGGT TATTTTAAAG 
CAATTGGAAG AGCATATGAA CACGGGCCAG CTAGCAGGAT TTTCACATCA AATTAGAAGT 
CTGATTTTGA ATAATATCAT CAATAAGAAG GAGTTTGGGA TTTTGGCAAA GACCAAATAC 
TTTCAAATGT TGAAGATGCA TGCGATGAAT ACCAACAATA TCACTGAGCT AGTGAACTAT 
TTGGCAAATG ACTTAAGTTT AGATGAAGCT TCAGTCTTGA TAACTGAATA TTCAAAGCAC 
TGCGGGAAAC CTGTGCCTCC AGACACTGCT CCCTGTGAAA TTCTGAAGAT GTTTCTTAGT 
GGATTATCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537329.1
CDS	146..6697
Translation	MWNDIELLTNDDTGSGYLSVGSRKEHGTALYQVDLLVKISSEKASLNPKIQACSLSDGFIIVADQSVILLDSICRSLQLHLVFDTEVDVVGLCQEGKFLLVGERSGNLHLIHVTSKQTLLTNAFVQKANDENRRTYQNLVIEKDGSNEGTYYMLLLTYSGFFCITNLQLLKIQQAIENVDFSTAKKLQGQIKSSFISTENYHTLGCLSLVAGDLASEVPVIIGGTGNCAFSKWEPDSSKKGMTVKNLIDAEIIKGAKKFQLIDNLLFVLDTDNVLSLWDIYTLTPVWNWPSLHVEEFLLTTEADSPSSVTWQGITNLKLIALTASANKKMKNLMVYSLPTMEILYSLEVSSVSSLVQTGISTDTIYLLEGVCKNDPKLSEDSVSVLVLRCLTEALPENRLSRLLHKHRFAEAESFAIQFGLDVELVYKVKSNHILEKLALSSVDASEQTEWQQLVDDAKENLHKIQDDEFVVNYCLKAQWITYETTQEMLNYAKTRLLKKEDKTALIYSDGLKEVLRAHAKLTTFYGAFGPEKFSGSSWIEFLNNEDDLKDIFLQLKEGNLVCAQYLWLRHRANFESRFDVKMLESLLNSMSASVSLQKLCPWFKNDVIPFVRRTVPEGQIILAKWLEQAARNLELTDKANWPENGLQLAEIFFTAEKTDELGLASSWHWISLKDYQNTEEVCQLRTLVNNLRELITLHRKYNCKLALSDFEKENTTTIVFRMFDKVLAPELIPSILEKFIRVYMREHDLQEEELLLLYIEDLLNRCSSKSTSLFETAWEAKAMAVIACLSDTDLIFDAVLKIMYAAVVPWSAAVEQLVKQHLEMDHPKVKLLQESYKLMEMKKLLRGYGIREVNLLNKEIMRVVRYILKQDVPSSLEDALKVAQAFMLSDDEIYSLRIIDLIDREQGEDCLLLLKSLPPAEAEKTAERVIIWARLALQEEPDHSKEGKAWRMSVAKTSVDILKILCDIQKDNLQKKDECEEMLKLFKEVASLQENFEVFLSFEDYSNSSLVADLREQHIKAHEVAQAKHKPGSTPEPIAAEVRSPSMESKLHRQALALQMSKQELEAELTLRALKDGNIKTALKKCSDLFKYHCNADTGKLLFLTCQKLCQMLADNVPVTVPVGLNLPSMIHDLASQAATICSPDFLLDALELCKHTLMAVELSRQCQMDDCGILMKASFGTHKDPYEEWSYSDFFSEDGIVLESQMVLPVIYELISSLVPLAESKRYPLESTSLPYCSLNEGDGLVLPVINSISALLQNLQESSQWELALRFVVGSFGTCLQHSVSNFMNATLSEKLFGETTLVKSRHVVMELKEKAVIFIRENATTLLHKVFNCRLVDLDLALGYCTLLPQKDVFENLWKLIDKAWQNYDKILAISLVGSELASLYQEIEMGLKFRELSTDAQWGIRLGKLGISFQPVFRQHFLTKKDLIKALVENIDMDTSLILEYCSTFQLDCDAVLQLFIETLLHNTNAGQGQGDASMDSAKRRHPKLLAKALEMVPLLTSTKDLVISLSGILHKLDPYDYEMIEVVLKVIERADEKITNININQALSILKHLKSYRRISPPVDLEYQYMLEHVITLPSAAQTRLPFHLIFFGTAQNFWKILSTELSEESFPTLLLISKLMKFSLDTLYVSTAKHVFEKKLKPKLLKLTQAKSSTLINKEITKITQTIESCLLSIVNPEWAVAIAISLAQDIPEGSFKISALKFCLYLAERWLQNIPSQDEKREKAEALLKKLHIQYRRSGTEAVLIAHKLNTEEYLRVIGKPAHLIVSLYEHPSINQRIQNSSGTDYPDIHAAAKEIAEVNEINLEKVWDMLLEKWLCPSTKPGEKPSELFELQEDEALRRVQYLLLSRPIDYSSRMLFVFATSTTTTLGMHQLTFAHRTRALQCLFYLADKETIESLFKKPIEEVNSPKEGMIKGLWKNHSHESMAVRLVTELCLEYKIYDLQLWNGLLQKLLGFNMIPYLRKVLKAISSIHSLWQVPYFSKAWQRVIQIPLLSASCPLSPDQLSDCSESLIAVLECPVSGDLDLIGVARQYIQLELPAFALACLMLMPHSEKRHQQIKNFLGSCDPQVILKQLEEHMNTGQLAGFSHQIRSLILNNIINKKEFGILAKTKYFQMLKMHAMNTNNITELVNYLANDLSLDEASVLITEYSKHCGKPVPPDTAPCEILKMFLSGLS

Target ORF information:

RefSeq Version	XM_011539027.2
Organism	Homo sapiens(human)
Definition	Homo sapiens kinetochore associated 1 (KNTC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539027.2

ORF Insert Sequence:

ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACCG GAAGTGGGTA CCTGAGTGTC 
GGTTCAAGAA AAGAACATGG AACTGCTTTA TATCAAGTAG ATTTGCTAGT GAAGATCTCT 
TCTGAAAAGG CCTCATTAAA TCCAAAGATA CAGGCATGCA GCTTAAGTGA TGGGTTTATT 
ATTGTAGCCG ACCAATCAGT GATATTGCTT GACAGTATTT GTAGATCACT TCAATTGCAT 
CTTGTCTTTG ATACTGAAGT GGATGTAGTT GGCCTTTGTC AAGAAGGAAA GTTTCTTTTG 
GTTGGCGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CATCAAAACA AACACTACTC 
ACTAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCGGC GGACTTACCA GAATCTTGTC 
ATTGAGAAGG ATGGTTCAAA TGAAGGTACC TATTATATGC TACTTCTTAC ATACAGTGGA 
TTTTTTTGTA TTACAAACCT TCAGCTTTTA AAAATTCAAC AAGCAATTGA GAATGTAGAC 
TTCAGTACAG CAAAAAAGTT ACAAGGACAA ATCAAGTCCA GTTTTATTTC TACTGAAAAT 
TATCATACTC TTGGTTGTCT CAGTCTTGTG GCTGGAGATT TAGCAAGTGA AGTTCCTGTG 
ATAATTGGGG GAACCGGTAA TTGTGCATTC TCAAAATGGG AACCAGATTC TTCCAAGAAA 
GGAATGACAG TTAAGAACCT TATTGATGCA GAGATTATTA AAGGTGCAAA GAAGTTCCAG 
CTGATAGACA ATCTACTTTT TGTTCTTGAT ACTGATAACG TGCTGAGTTT ATGGGATATT 
TACACTCTAA CTCCTGTATG GAACTGGCCC TCTCTTCACG TAGAAGAGTT TCTTCTTACT 
ACAGAAGCAG ACTCTCCTTC ATCAGTCACG TGGCAAGGAA TTACAAATCT CAAATTAATA 
GCTCTGACAG CTTCAGCTAA TAAGAAGATG AAAAACCTCA TGGTTTATTC ATTACCTACA 
ATGGAAATAC TATATTCTTT GGAAGTATCT AGTGTTTCTT CTCTGGTCCA AACAGGAATT 
AGCACAGATA CCATATACCT TTTAGAAGGA GTTTGCAAAA ATGATCCAAA ATTGTCTGAA 
GACTCAGTCT CTGTGTTAGT ACTCAGATGT CTTACGGAAG CTTTACCAGA AAACAGATTG 
AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GTTTTGCCAT TCAGTTTGGA 
CTAGATGTTG AGCTTGTTTA CAAGGTCAAG TCAAATCATA TATTGGAGAA ACTGGCATTG 
AGTTCTGTGG ATGCCAGTGA ACAGACCGAA TGGCAACAAC TTGTAGACGA CGCTAAGGAA 
AATCTACATA AGATCCAGGA TGATGAATTT GTGGTGAATT ACTGCCTGAA AGCTCAGTGG 
ATAACCTATG AAACCACTCA AGAGATGCTG AATTATGCCA AAACCAGGCT TTTGAAGAAA 
GAAGATAAAA CTGCTCTCAT TTATTCTGAT GGCTTGAAAG AGGTGCTAAG AGCTCATGCA 
AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGCAG TTCTTGGATT 
GAATTTCTAA ATAATGAAGA TGATCTTAAA GATATTTTTT TACAGCTAAA AGAAGGAAAC 
CTTGTTTGTG CACAGTATCT TTGGCTTCGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT 
GTGAAAATGC TGGAGAGCTT GCTCAACTCA ATGTCTGCAT CAGTCTCTTT GCAAAAGCTG 
TGTCCATGGT TTAAAAATGA TGTGATTCCA TTTGTAAGAA GGACTGTGCC TGAAGGACAG 
ATAATTCTTG CAAAATGGTT GGAACAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA 
AATTGGCCAG AAAATGGACT TCAATTGGCA GAGATATTTT TTACAGCAGA AAAAACAGAC 
GAGTTGGGAT TGGCATCTTC CTGGCATTGG ATTTCCTTGA AAGATTATCA GAACACAGAG 
GAAGTATGTC AGCTAAGGAC TTTGGTAAAT AACTTGCGAG AGTTGATCAC GTTGCATAGG 
AAGTACAACT GCAAATTAGC CCTCTCTGAT TTTGAGAAGG AAAATACAAC CACCATAGTG 
TTCCGAATGT TTGATAAAGT GCTGGCCCCA GAGCTTATTC CCTCCATCTT AGAGAAGTTT 
ATAAGAGTTT ACATGAGAGA ACATGACTTG CAAGAGGAGG AACTTCTCTT GCTGTACATA 
GAGGATTTAC TGAATAGATG CAGCTCAAAG TCCACATCAC TCTTTGAAAC AGCATGGGAA 
GCAAAGGCCA TGGCAGTAAT AGCGTGTTTA TCTGACACGG ACCTCATATT TGATGCCGTG 
CTCAAGATCA TGTATGCGGC AGTGGTTCCT TGGAGTGCAG CTGTGGAGCA ACTGGTGAAA 
CAGCACCTGG AAATGGACCA TCCCAAAGTC AAGTTATTAC AGGAAAGTTA CAAACTAATG 
GAGATGAAAA AACTTTTACG AGGCTATGGA ATAAGAGAGG TAAATCTCTT AAACAAGGAA 
ATAATGAGAG TGGTTAGATA CATTCTCAAA CAAGATGTCC CATCTTCTTT AGAAGATGCT 
TTAAAGGTAG CCCAAGCGTT TATGTTATCT GATGATGAGA TCTACAGTCT AAGAATTATT 
GACCTGATTG ATAGAGAACA GGGTGAAGAC TGTCTCCTTC TGTTGAAGTC TTTGCCTCCT 
GCTGAAGCTG AGAAAACTGC AGAAAGAGTC ATCATATGGG CACGACTGGC ATTACAAGAA 
GAGCCAGATC ATTCTAAAGA GGGCAAGGCC TGGAGAATGT CTGTAGCGAA GACATCCGTG 
GACATTCTTA AGATACTATG TGACATTCAG AAAGACAATC TGCAGAAGAA GGACGAATGT 
GAAGAAATGT TGAAACTATT TAAAGAGGTT GCTAGCTTAC AGGAGAACTT TGAGGTCTTT 
CTTTCATTTG AAGATTATAG CAATAGTTCC CTGGTAGCAG ATCTCCGTGA GCAGCACATT 
AAAGCTCACG AAGTTGCACA GGCGAAACAC AAACCTGGGA GCACCCCAGA GCCCATAGCT 
GCTGAGGTGA GGAGCCCAAG CATGGAATCA AAGCTGCACA GACAGGCACT GGCCCTGCAG 
ATGTCCAAAC AAGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAAGA TGGGAACATC 
AAAACAGCAC TGAAAAAATG CAGCGACTTG TTTAAGTATC ACTGCAATGC TGACACTGGG 
AAATTGCTAT TTCTGACATG TCAGAAGCTT TGTCAGATGT TGGCTGATAA TGTCCCAGTG 
ACAGTGCCTG TGGGACTGAA TCTTCCTTCC ATGATACATG ATCTAGCAAG CCAAGCTGCC 
ACCATTTGCA GTCCAGATTT TTTACTAGAT GCTTTAGAAC TATGTAAACA TACTTTAATG 
GCTGTAGAGC TTTCCAGACA ATGCCAAATG GATGACTGTG GAATCCTCAT GAAAGCTTCT 
TTTGGGACAC ATAAAGATCC ATATGAAGAG TGGTCTTACA GTGACTTCTT CAGTGAAGAT 
GGAATTGTTC TTGAGTCACA GATGGTGCTT CCAGTGATTT ATGAACTGAT TTCATCTCTT 
GTGCCTCTAG CTGAAAGCAA GAGATATCCC TTGGAGTCTA CCAGTTTGCC ATACTGCTCC 
CTTAATGAAG GAGATGGCCT TGTTTTACCT GTTATAAATT CCATCTCTGC CCTGCTTCAG 
AATCTTCAGG AATCTAGCCA GTGGGAGCTA GCCCTAAGAT TTGTGGTTGG TTCATTTGGT 
ACCTGTCTTC AGCACTCTGT GTCAAACTTC ATGAATGCCA CTTTGAGTGA AAAGTTATTT 
GGAGAGACTA CATTAGTTAA ATCAAGGCAT GTTGTTATGG AATTGAAAGA AAAAGCTGTT 
ATATTTATCA GGGAAAATGC TACAACACTA CTGCACAAAG TATTTAATTG TCGCTTGGTA 
GATCTTGACC TGGCGTTGGG TTACTGCACT CTCTTACCTC AAAAAGATGT GTTTGAAAAT 
CTCTGGAAGC TCATAGATAA AGCATGGCAG AATTACGACA AAATCTTGGC AATATCTCTG 
GTGGGCTCTG AGCTGGCAAG TCTCTATCAG GAAATAGAAA TGGGGCTTAA GTTCCGTGAA 
CTCAGTACTG ATGCCCAGTG GGGCATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAACCA 
GTTTTCAGGC AACATTTTCT CACCAAGAAA GACCTCATTA AAGCTCTTGT GGAGAATATA 
GATATGGACA CAAGCCTCAT TTTGGAATAT TGCAGCACAT TTCAGTTGGA CTGCGATGCA 
GTTCTTCAGC TCTTCATTGA AACGCTGCTC CACAACACAA ATGCCGGCCA AGGCCAGGGA 
GATGCAAGCA TGGACTCTGC AAAGCGGCGG CATCCCAAAC TCCTGGCCAA AGCCCTTGAG 
ATGGTTCCTT TACTGACGAG CACAAAAGAT TTGGTCATCA GTCTTAGTGG AATACTACAT 
AAGTTGGATC CTTATGACTA TGAAATGATT GAAGTTGTCT TGAAAGTTAT AGAACGAGCT 
GATGAAAAGA TAACCAATAT TAATATTAAT CAGGCATTGA GTATTCTGAA ACATTTGAAG 
TCATACAGAA GAATTTCTCC TCCCGTGGAT CTAGAATATC AGTATATGTT GGAACATGTC 
ATAACTTTGC CATCAGCTGC CCAAACTAGA CTGCCTTTTC ACCTGATATT CTTTGGCACA 
GCACAGAACT TCTGGAAAAT TCTCTCTACA GAACTCAGTG AAGAATCTTT CCCAACATTG 
CTCTTAATTT CGAAATTAAT GAAGTTCTCT CTGGACACTC TGTACGTGTC TACAGCAAAA 
CACGTTTTCG AAAAAAAACT GAAGCCAAAG CTCCTGAAGT TAACACAAGC TAAATCCTCA 
ACACTGATTA ACAAGGAAAT AACTAAGATC ACGCAGACCA TCGAATCCTG CTTACTCTCT 
ATAGTCAACC CAGAGTGGGC TGTAGCTATT GCCATCAGCC TTGCCCAGGA TATCCCTGAA 
GGTTCCTTCA AGATATCTGC TTTGAAATTC TGCCTTTATT TAGCTGAGAG ATGGCTACAG 
AATATCCCAT CGCAGGACGA AAAACGTGAA AAAGCCGAGG CTTTGTTGAA GAAGCTTCAT 
ATCCAGTACC GGCGATCGGG CACAGAAGCT GTGCTCATAG CCCACAAGCT GAACACTGAG 
GAATATTTAA GAGTGATCGG AAAGCCAGCA CATCTTATTG TCAGTCTCTA CGAACATCCT 
AGCATCAATC AAAGAATTCA GAATTCATCT GGCACAGATT ATCCTGATAT TCATGCAGCA 
GCTAAAGAAA TAGCCGAAGT CAATGAAATT AATTTGGAAA AAGTCTGGGA CATGTTGTTG 
GAAAAATGGC TATGCCCTTC AACAAAACCT GGTGAAAAAC CATCAGAATT ATTTGAACTT 
CAAGAAGATG AAGCCCTACG AAGAGTGCAG TATCTCCTCC TGTCTCGTCC AATTGATTAT 
AGTTCAAGAA TGCTGTTTGT ATTTGCAACA TCAACTACAA CCACATTAGG TATGCATCAG 
TTAACTTTTG CCCATAGAAC TCGAGCTCTT CAGTGTCTCT TCTATTTGGC TGACAAGGAA 
ACTATAGAAT CTCTCTTTAA AAAACCCATT GAAGAAGTGA ACAGTCCTAA AGAAGGAATG 
ATTAAGGGTC TGTGGAAAAA CCACAGCCAC GAGTCCATGG CAGTAAGATT GGTGACTGAG 
CTGTGTTTAG AATACAAAAT CTATGACCTG CAGCTTTGGA ATGGACTCTT GCAAAAGCTT 
CTGGGCTTCA ATATGATTCC TTATCTAAGG AAAGTTTTAA AAGCCATCTC CAGTATCCAT 
TCTTTATGGC AGGTTCCCTA CTTCAGCAAA GCGTGGCAGC GTGTGATACA GATACCACTG 
CTTTCAGCCT CTTGTCCTTT AAGTCCTGAT CAGCTGTCAG ATTGTTCTGA GAGTCTCATC 
GCTGTCCTCG AATGTCCAGT CTCAGGTGAT CTTGACCTGA TCGGAGTCGC CAGGCAGTAT 
ATCCAGTTAG AACTTCCGGC TTTTGCATTA GCTTGTCTGA TGCTCATGCC CCACTCAGAG 
AAAAGACACC AGCAAATTAA GAATTTTCTG GGTTCCTGTG ACCCTCAGGT TATTTTAAAG 
CAATTGGAAG AGCATATGAA CACGGGCCAG CTAGCAGGAT TTTCACATCA AATTAGAAGT 
CTGATTTTGA ATAATATCAT CAATAAGAAG GAGTTTGGGA TTTTGGCAAA GACCAAATAC 
TTTCAAATGT TGAAGATGCA TGCGATGAAT ACCAACAATA TCACTGAGCT AGTGAACTAT 
TTGGCAAATG ACTTAAGTTT AGATGAAGCT TCAGTCTTGA TAACTGAATA TTCAAAGCAC 
TGCGGGAAAC CTGTGCCTCC AGACACTGCT CCCTGTGAAA TTCTGAAGAT GTTTCTTAGT 
GGATTATCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	KNTC1
Entrez Gene ID	9735
Full Name	kinetochore associated 1
Synonyms	ROD
General protein information	Preferred Names kinetochore associated 1 Names kinetochore-associated protein 1 Rough Deal homolog, centromere/kinetochore protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.31
Summary	This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. Experimental evidence indicated that the encoded protein functioned in a similar manner to that of the Drosophila rough deal protein. [provided by RefSeq, Jul 2008].
Disorder MIM:	607363

Homo sapiens (human)	KNTC1	NP_055523.1
Bos taurus (cattle)	KNTC1	NP_001179020.2
Rattus norvegicus (Norway rat)	Kntc1	NP_001100610.1
Xenopus tropicalis (tropical clawed frog)	kntc1	XP_002935241.2
Gallus gallus (chicken)	KNTC1	XP_415157.4
Danio rerio (zebrafish)	kntc1	XP_686358.6
Mus musculus (house mouse)	Kntc1	NP_001035886.1
	KNTC1	XP_002808044.1
Pan troglodytes (chimpanzee)	KNTC1	XP_001168340.1
Canis lupus familiaris (dog)	KNTC1	XP_005636213.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

KNTC1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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