RNF185 cDNA ORF clone, Homo sapiens(Human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RNF185
Entrez Gene ID	91445
Full Name	ring finger protein 185
General protein information	Preferred Names ring finger protein 185 Names E3 ubiquitin-protein ligase RNF185 BSK65-MONO1 BSK65-MONO2 BSK65-PANC1 BSK65-PANC2 BSK65-TEST1 BSK65-TEST2 BSK65-TEST3 RING-type E3 ubiquitin transferase RNF185
Gene Type	protein-coding
Organism	Homo sapiens(Human)
Genome	Chromosome: 22 Map Location: 22q12.2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001135825.2	NP_001129297.1	E3 ubiquitin-protein ligase RNF185 isoform 2
NM_152267.3	NP_689480.2	E3 ubiquitin-protein ligase RNF185 isoform 1
NM_001135825.1	NP_001129297.1	E3 ubiquitin-protein ligase RNF185 isoform 2
NM_152267.4	NP_689480.2	E3 ubiquitin-protein ligase RNF185 isoform 1
XM_006724374.2	XP_006724437.1	E3 ubiquitin-protein ligase RNF185 isoform X1
XM_006724373.2	XP_006724436.1	E3 ubiquitin-protein ligase RNF185 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	RNF185	NP_689480.2
Caenorhabditis elegans (roundworm)	rnf-5	NP_497830.1
Bos taurus (cattle)	RNF185	NP_001077172.1
Danio rerio (zebrafish)	rnf185	NP_998202.1
Pan troglodytes (chimpanzee)	RNF185	XP_515084.1
Canis lupus familiaris (dog)	RNF185	XP_852634.1
Rattus norvegicus (Norway rat)	Rnf185	NP_001019442.1
Gallus gallus (chicken)	RNF185	NP_001007841.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006041	XP_316088.3
Mus musculus (house mouse)	Rnf185	NP_663330.2
Drosophila melanogaster (fruit fly)	CG8974	NP_727897.1
Drosophila melanogaster (fruit fly)	CG32581	NP_727898.2

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

The E3 ubiquitin ligase RNF185 facilitates the cGAS-mediated innate immune response.
Wang Q, Huang L, Hong Z, Lv Z, Mao Z, Tang Y, Kong X, Li S, Cui Y, Liu H, Zhang L, Zhang X, Jiang L, Wang C, Zhou Q
PLoS pathogens13(3)e1006264(2017 Mar)

Genome-wide identification and gene expression profiling of ubiquitin ligases for endoplasmic reticulum protein degradation.
Kaneko M, Iwase I, Yamasaki Y, Takai T, Wu Y, Kanemoto S, Matsuhisa K, Asada R, Okuma Y, Watanabe T, Imaizumi K, Nomura Y
Scientific reports630955(2016 Aug)

The E3 ligase RNF185 negatively regulates osteogenic differentiation by targeting Dvl2 for degradation.
Zhou Y, Shang H, Zhang C, Liu Y, Zhao Y, Shuang F, Zhong H, Tang J, Hou S
Biochemical and biophysical research communications447(3)431-6(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study uncovers RNF185 as the first E3 ubiquitin ligase of cyclic GMP-AMP synthase (cGAS), shedding light on the regulation of cGAS activity in innate immune responses
Title: The E3 ubiquitin ligase RNF185 facilitates the cGAS-mediated innate immune response.

these results indicate that RNF185 negatively regulates osteogenesis through the degradation of Dvl2 and down-regulation of canonical Wnt signaling pathway and suggest a possible therapeutic target in osteoporosis.
Title: The E3 ligase RNF185 negatively regulates osteogenic differentiation by targeting Dvl2 for degradation.

data thus identify RNF185 and RNF5 as a novel E3 ligase module that is central to the control of CFTR degradation.
Title: RNF185 is a novel E3 ligase of endoplasmic reticulum-associated degradation (ERAD) that targets cystic fibrosis transmembrane conductance regulator (CFTR).

Human BNIP1 colocalizes with RNF185 at mitochondria and is polyubiquitinated by RNF185 through K63-based ubiquitin linkage in vivo.
Title: RNF185, a novel mitochondrial ubiquitin E3 ligase, regulates autophagy through interaction with BNIP1.

The following RNF185 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNF185 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31573	NM_152267.4 Latest version!	Homo sapiens ring finger protein 185 (RNF185), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu31573	XM_006724374.2 Latest version!	Homo sapiens ring finger protein 185 (RNF185), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu31573	XM_006724373.2 Latest version!	Homo sapiens ring finger protein 185 (RNF185), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu07362	NM_001135825.2 Latest version!	Homo sapiens ring finger protein 185 (RNF185), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu07362	NM_001135825.1	Homo sapiens ring finger protein 185 (RNF185), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu31573	NM_152267.3	Homo sapiens ring finger protein 185 (RNF185), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNF185 ( NM_152267.3 ) cDNA ORF clone, Homo sapiens(Human) -> NP_689480.2 Homo sapiens ring finger protein 185 (RNF185), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31573
Clone ID Related Accession (Same CDS sequence)	NM_152267.3 , NM_152267.4 , XM_006724374.2 , XM_006724373.2
Accession Version	NM_152267.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-30
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF185 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB134817.1, BC033166.1 and AA258379.1. On Oct 16, 2008 this sequence version replaced NM_152267.2. Transcript Variant: This variant (1) represents the predominant transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1803611.85764.1, SRR1803612.47633.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGCA AGGGGCCCTC GGCCTCTGCA TCTCCTGAGA ACTCCAGTGC AGGGGGGCCC 
AGTGGGAGCA GCAATGGCGC TGGCGAGAGC GGAGGGCAGG ACAGCACTTT CGAGTGCAAC 
ATCTGCTTGG ACACAGCCAA GGATGCCGTC ATCAGCCTGT GTGGCCACCT CTTCTGTTGG 
CCGTGTTTAC ATCAGTGGTT GGAGACCAGA CCTAACAGAC AGGTGTGTCC TGTTTGCAAA 
GCTGGCATCA GCCGAGACAA GGTCATCCCC CTCTATGGAA GGGGCAGCAC TGGGCAACAG 
GACCCCAGAG AGAAGACCCC TCCTCGTCCT CAAGGACAGA GGCCAGAGCC GGAGAATAGA 
GGGGGATTTC AAGGATTTGG ATTTGGAGAT GGTGGCTTCC AGATGTCTTT TGGAATTGGG 
GCATTTCCCT TTGGGATATT TGCCACAGCA TTTAATATAA ATGATGGGCG GCCTCCTCCA 
GCTGTCCCTG GGACACCCCA GTATGTGGAC GAGCAGTTCC TGTCACGCCT CTTCCTATTT 
GTGGCCCTGG TGATCATGTT CTGGCTCCTG ATTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689480.2
CDS	201..779
Translation	MASKGPSASASPENSSAGGPSGSSNGAGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKTPPRPQGQRPEPENRGGFQGFGFGDGGFQMSFGIGAFPFGIFATAFNINDGRPPPAVPGTPQYVDEQFLSRLFLFVALVIMFWLLIA

Target ORF information:

RefSeq Version	NM_152267.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 185 (RNF185), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152267.3

ORF Insert Sequence:

ATGGCAAGCA AGGGGCCCTC GGCCTCTGCA TCTCCTGAGA ACTCCAGTGC AGGGGGGCCC 
AGTGGGAGCA GCAATGGCGC TGGCGAGAGC GGAGGGCAGG ACAGCACTTT CGAGTGCAAC 
ATCTGCTTGG ACACAGCCAA GGATGCCGTC ATCAGCCTGT GTGGCCACCT CTTCTGTTGG 
CCGTGTTTAC ATCAGTGGTT GGAGACCAGA CCTAACAGAC AGGTGTGTCC TGTTTGCAAA 
GCTGGCATCA GCCGAGACAA GGTCATCCCC CTCTATGGAA GGGGCAGCAC TGGGCAACAG 
GACCCCAGAG AGAAGACCCC TCCTCGTCCT CAAGGACAGA GGCCAGAGCC GGAGAATAGA 
GGGGGATTTC AAGGATTTGG ATTTGGAGAT GGTGGCTTCC AGATGTCTTT TGGAATTGGG 
GCATTTCCCT TTGGGATATT TGCCACAGCA TTTAATATAA ATGATGGGCG GCCTCCTCCA 
GCTGTCCCTG GGACACCCCA GTATGTGGAC GAGCAGTTCC TGTCACGCCT CTTCCTATTT 
GTGGCCCTGG TGATCATGTT CTGGCTCCTG ATTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF185 ( NM_152267.4 ) cDNA ORF clone, Homo sapiens(Human) -> NP_689480.2 Homo sapiens ring finger protein 185 (RNF185), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31573
Clone ID Related Accession (Same CDS sequence)	NM_152267.3 , NM_152267.4 , XM_006724374.2 , XM_006724373.2
Accession Version	NM_152267.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF185 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB134817.1, BC033166.1 and AA258379.1. On Nov 23, 2018 this sequence version replaced NM_152267.3. Transcript Variant: This variant (1) represents the predominant transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1803613.215631.1, SRR1803611.272208.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000326132.11/ ENSP00000320508.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCAAGCA AGGGGCCCTC GGCCTCTGCA TCTCCTGAGA ACTCCAGTGC AGGGGGGCCC 
AGTGGGAGCA GCAATGGCGC TGGCGAGAGC GGAGGGCAGG ACAGCACTTT CGAGTGCAAC 
ATCTGCTTGG ACACAGCCAA GGATGCCGTC ATCAGCCTGT GTGGCCACCT CTTCTGTTGG 
CCGTGTTTAC ATCAGTGGTT GGAGACCAGA CCTAACAGAC AGGTGTGTCC TGTTTGCAAA 
GCTGGCATCA GCCGAGACAA GGTCATCCCC CTCTATGGAA GGGGCAGCAC TGGGCAACAG 
GACCCCAGAG AGAAGACCCC TCCTCGTCCT CAAGGACAGA GGCCAGAGCC GGAGAATAGA 
GGGGGATTTC AAGGATTTGG ATTTGGAGAT GGTGGCTTCC AGATGTCTTT TGGAATTGGG 
GCATTTCCCT TTGGGATATT TGCCACAGCA TTTAATATAA ATGATGGGCG GCCTCCTCCA 
GCTGTCCCTG GGACACCCCA GTATGTGGAC GAGCAGTTCC TGTCACGCCT CTTCCTATTT 
GTGGCCCTGG TGATCATGTT CTGGCTCCTG ATTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689480.2
CDS	171..749
Translation	MASKGPSASASPENSSAGGPSGSSNGAGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKTPPRPQGQRPEPENRGGFQGFGFGDGGFQMSFGIGAFPFGIFATAFNINDGRPPPAVPGTPQYVDEQFLSRLFLFVALVIMFWLLIA

Target ORF information:

RefSeq Version	NM_152267.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 185 (RNF185), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152267.4

ORF Insert Sequence:

ATGGCAAGCA AGGGGCCCTC GGCCTCTGCA TCTCCTGAGA ACTCCAGTGC AGGGGGGCCC 
AGTGGGAGCA GCAATGGCGC TGGCGAGAGC GGAGGGCAGG ACAGCACTTT CGAGTGCAAC 
ATCTGCTTGG ACACAGCCAA GGATGCCGTC ATCAGCCTGT GTGGCCACCT CTTCTGTTGG 
CCGTGTTTAC ATCAGTGGTT GGAGACCAGA CCTAACAGAC AGGTGTGTCC TGTTTGCAAA 
GCTGGCATCA GCCGAGACAA GGTCATCCCC CTCTATGGAA GGGGCAGCAC TGGGCAACAG 
GACCCCAGAG AGAAGACCCC TCCTCGTCCT CAAGGACAGA GGCCAGAGCC GGAGAATAGA 
GGGGGATTTC AAGGATTTGG ATTTGGAGAT GGTGGCTTCC AGATGTCTTT TGGAATTGGG 
GCATTTCCCT TTGGGATATT TGCCACAGCA TTTAATATAA ATGATGGGCG GCCTCCTCCA 
GCTGTCCCTG GGACACCCCA GTATGTGGAC GAGCAGTTCC TGTCACGCCT CTTCCTATTT 
GTGGCCCTGG TGATCATGTT CTGGCTCCTG ATTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF185 ( XM_006724374.2 ) cDNA ORF clone, Homo sapiens(Human) -> XP_006724437.1 Homo sapiens ring finger protein 185 (RNF185), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu31573
Clone ID Related Accession (Same CDS sequence)	NM_152267.3 , NM_152267.4 , XM_006724374.2 , XM_006724373.2
Accession Version	XM_006724374.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	E3 ubiquitin-protein ligase RNF185 isoform X1
Comment

ATGGCAAGCA AGGGGCCCTC GGCCTCTGCA TCTCCTGAGA ACTCCAGTGC AGGGGGGCCC 
AGTGGGAGCA GCAATGGCGC TGGCGAGAGC GGAGGGCAGG ACAGCACTTT CGAGTGCAAC 
ATCTGCTTGG ACACAGCCAA GGATGCCGTC ATCAGCCTGT GTGGCCACCT CTTCTGTTGG 
CCGTGTTTAC ATCAGTGGTT GGAGACCAGA CCTAACAGAC AGGTGTGTCC TGTTTGCAAA 
GCTGGCATCA GCCGAGACAA GGTCATCCCC CTCTATGGAA GGGGCAGCAC TGGGCAACAG 
GACCCCAGAG AGAAGACCCC TCCTCGTCCT CAAGGACAGA GGCCAGAGCC GGAGAATAGA 
GGGGGATTTC AAGGATTTGG ATTTGGAGAT GGTGGCTTCC AGATGTCTTT TGGAATTGGG 
GCATTTCCCT TTGGGATATT TGCCACAGCA TTTAATATAA ATGATGGGCG GCCTCCTCCA 
GCTGTCCCTG GGACACCCCA GTATGTGGAC GAGCAGTTCC TGTCACGCCT CTTCCTATTT 
GTGGCCCTGG TGATCATGTT CTGGCTCCTG ATTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006724437.1
Translation	MASKGPSASASPENSSAGGPSGSSNGAGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKTPPRPQGQRPEPENRGGFQGFGFGDGGFQMSFGIGAFPFGIFATAFNINDGRPPPAVPGTPQYVDEQFLSRLFLFVALVIMFWLLIA

Target ORF information:

RefSeq Version	XM_006724374.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens ring finger protein 185 (RNF185), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006724374.2

ORF Insert Sequence:

ATGGCAAGCA AGGGGCCCTC GGCCTCTGCA TCTCCTGAGA ACTCCAGTGC AGGGGGGCCC 
AGTGGGAGCA GCAATGGCGC TGGCGAGAGC GGAGGGCAGG ACAGCACTTT CGAGTGCAAC 
ATCTGCTTGG ACACAGCCAA GGATGCCGTC ATCAGCCTGT GTGGCCACCT CTTCTGTTGG 
CCGTGTTTAC ATCAGTGGTT GGAGACCAGA CCTAACAGAC AGGTGTGTCC TGTTTGCAAA 
GCTGGCATCA GCCGAGACAA GGTCATCCCC CTCTATGGAA GGGGCAGCAC TGGGCAACAG 
GACCCCAGAG AGAAGACCCC TCCTCGTCCT CAAGGACAGA GGCCAGAGCC GGAGAATAGA 
GGGGGATTTC AAGGATTTGG ATTTGGAGAT GGTGGCTTCC AGATGTCTTT TGGAATTGGG 
GCATTTCCCT TTGGGATATT TGCCACAGCA TTTAATATAA ATGATGGGCG GCCTCCTCCA 
GCTGTCCCTG GGACACCCCA GTATGTGGAC GAGCAGTTCC TGTCACGCCT CTTCCTATTT 
GTGGCCCTGG TGATCATGTT CTGGCTCCTG ATTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF185 ( XM_006724373.2 ) cDNA ORF clone, Homo sapiens(Human) -> XP_006724436.1 Homo sapiens ring finger protein 185 (RNF185), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu31573
Clone ID Related Accession (Same CDS sequence)	NM_152267.3 , NM_152267.4 , XM_006724374.2 , XM_006724373.2
Accession Version	XM_006724373.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	E3 ubiquitin-protein ligase RNF185 isoform X1
Comment

ATGGCAAGCA AGGGGCCCTC GGCCTCTGCA TCTCCTGAGA ACTCCAGTGC AGGGGGGCCC 
AGTGGGAGCA GCAATGGCGC TGGCGAGAGC GGAGGGCAGG ACAGCACTTT CGAGTGCAAC 
ATCTGCTTGG ACACAGCCAA GGATGCCGTC ATCAGCCTGT GTGGCCACCT CTTCTGTTGG 
CCGTGTTTAC ATCAGTGGTT GGAGACCAGA CCTAACAGAC AGGTGTGTCC TGTTTGCAAA 
GCTGGCATCA GCCGAGACAA GGTCATCCCC CTCTATGGAA GGGGCAGCAC TGGGCAACAG 
GACCCCAGAG AGAAGACCCC TCCTCGTCCT CAAGGACAGA GGCCAGAGCC GGAGAATAGA 
GGGGGATTTC AAGGATTTGG ATTTGGAGAT GGTGGCTTCC AGATGTCTTT TGGAATTGGG 
GCATTTCCCT TTGGGATATT TGCCACAGCA TTTAATATAA ATGATGGGCG GCCTCCTCCA 
GCTGTCCCTG GGACACCCCA GTATGTGGAC GAGCAGTTCC TGTCACGCCT CTTCCTATTT 
GTGGCCCTGG TGATCATGTT CTGGCTCCTG ATTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006724436.1
Translation	MASKGPSASASPENSSAGGPSGSSNGAGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKTPPRPQGQRPEPENRGGFQGFGFGDGGFQMSFGIGAFPFGIFATAFNINDGRPPPAVPGTPQYVDEQFLSRLFLFVALVIMFWLLIA

Target ORF information:

RefSeq Version	XM_006724373.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens ring finger protein 185 (RNF185), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006724373.2

ORF Insert Sequence:

ATGGCAAGCA AGGGGCCCTC GGCCTCTGCA TCTCCTGAGA ACTCCAGTGC AGGGGGGCCC 
AGTGGGAGCA GCAATGGCGC TGGCGAGAGC GGAGGGCAGG ACAGCACTTT CGAGTGCAAC 
ATCTGCTTGG ACACAGCCAA GGATGCCGTC ATCAGCCTGT GTGGCCACCT CTTCTGTTGG 
CCGTGTTTAC ATCAGTGGTT GGAGACCAGA CCTAACAGAC AGGTGTGTCC TGTTTGCAAA 
GCTGGCATCA GCCGAGACAA GGTCATCCCC CTCTATGGAA GGGGCAGCAC TGGGCAACAG 
GACCCCAGAG AGAAGACCCC TCCTCGTCCT CAAGGACAGA GGCCAGAGCC GGAGAATAGA 
GGGGGATTTC AAGGATTTGG ATTTGGAGAT GGTGGCTTCC AGATGTCTTT TGGAATTGGG 
GCATTTCCCT TTGGGATATT TGCCACAGCA TTTAATATAA ATGATGGGCG GCCTCCTCCA 
GCTGTCCCTG GGACACCCCA GTATGTGGAC GAGCAGTTCC TGTCACGCCT CTTCCTATTT 
GTGGCCCTGG TGATCATGTT CTGGCTCCTG ATTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF185 ( NM_001135825.2 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001129297.1 Homo sapiens ring finger protein 185 (RNF185), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu07362
Clone ID Related Accession (Same CDS sequence)	NM_001135825.2 , NM_001135825.1
Accession Version	NM_001135825.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF185 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB134817.1, DQ296562.1 and AA258379.1. On May 31, 2019 this sequence version replaced NM_001135825.1. Transcript Variant: This variant (3) is lacking two in-frame coding exons compared to transcript variant 1, resulting in a shorter isoform (2) missing a 56 aa protein segment compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: DQ296562.1, SRR1660807.105978.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGCA AGGGGCCCTC GGCCTCTGCA TCTCCTGAGA ACTCCAGTGC AGGGGGGCCC 
AGTGGGAGCA GCAATGGCGC TGGCGAGAGC GGAGGGCAGG ACAGCACTTT CGAGTGCAAC 
ATCTGCTTGG ACACAGCCAA GGATGCCGTC ATCAGCCTGT GTGGCCACCT CTTCTGTTGG 
CCGTGTTTAC ATCAGGGATT TCAAGGATTT GGATTTGGAG ATGGTGGCTT CCAGATGTCT 
TTTGGAATTG GGGCATTTCC CTTTGGGATA TTTGCCACAG CATTTAATAT AAATGATGGG 
CGGCCTCCTC CAGCTGTCCC TGGGACACCC CAGTATGTGG ACGAGCAGTT CCTGTCACGC 
CTCTTCCTAT TTGTGGCCCT GGTGATCATG TTCTGGCTCC TGATTGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129297.1
CDS	171..581
Translation	MASKGPSASASPENSSAGGPSGSSNGAGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLHQGFQGFGFGDGGFQMSFGIGAFPFGIFATAFNINDGRPPPAVPGTPQYVDEQFLSRLFLFVALVIMFWLLIA

Target ORF information:

RefSeq Version	NM_001135825.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 185 (RNF185), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135825.2

ORF Insert Sequence:

ATGGCAAGCA AGGGGCCCTC GGCCTCTGCA TCTCCTGAGA ACTCCAGTGC AGGGGGGCCC 
AGTGGGAGCA GCAATGGCGC TGGCGAGAGC GGAGGGCAGG ACAGCACTTT CGAGTGCAAC 
ATCTGCTTGG ACACAGCCAA GGATGCCGTC ATCAGCCTGT GTGGCCACCT CTTCTGTTGG 
CCGTGTTTAC ATCAGGGATT TCAAGGATTT GGATTTGGAG ATGGTGGCTT CCAGATGTCT 
TTTGGAATTG GGGCATTTCC CTTTGGGATA TTTGCCACAG CATTTAATAT AAATGATGGG 
CGGCCTCCTC CAGCTGTCCC TGGGACACCC CAGTATGTGG ACGAGCAGTT CCTGTCACGC 
CTCTTCCTAT TTGTGGCCCT GGTGATCATG TTCTGGCTCC TGATTGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF185 ( NM_001135825.1 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001129297.1 Homo sapiens ring finger protein 185 (RNF185), transcript variant 3, mRNA.

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CloneID	OHu07362
Clone ID Related Accession (Same CDS sequence)	NM_001135825.2 , NM_001135825.1
Accession Version	NM_001135825.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF185 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB134817.1, DQ296562.1 and AA258379.1. Transcript Variant: This variant (3) is lacking two in-frame coding exons compared to transcript variant 1, resulting in a shorter isoform (2) missing a 56 aa protein segment compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.275208.1, SRR1803614.180115.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGCA AGGGGCCCTC GGCCTCTGCA TCTCCTGAGA ACTCCAGTGC AGGGGGGCCC 
AGTGGGAGCA GCAATGGCGC TGGCGAGAGC GGAGGGCAGG ACAGCACTTT CGAGTGCAAC 
ATCTGCTTGG ACACAGCCAA GGATGCCGTC ATCAGCCTGT GTGGCCACCT CTTCTGTTGG 
CCGTGTTTAC ATCAGGGATT TCAAGGATTT GGATTTGGAG ATGGTGGCTT CCAGATGTCT 
TTTGGAATTG GGGCATTTCC CTTTGGGATA TTTGCCACAG CATTTAATAT AAATGATGGG 
CGGCCTCCTC CAGCTGTCCC TGGGACACCC CAGTATGTGG ACGAGCAGTT CCTGTCACGC 
CTCTTCCTAT TTGTGGCCCT GGTGATCATG TTCTGGCTCC TGATTGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129297.1
CDS	201..611
Translation	MASKGPSASASPENSSAGGPSGSSNGAGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLHQGFQGFGFGDGGFQMSFGIGAFPFGIFATAFNINDGRPPPAVPGTPQYVDEQFLSRLFLFVALVIMFWLLIA

Target ORF information:

RefSeq Version	NM_001135825.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 185 (RNF185), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135825.1

ORF Insert Sequence:

ATGGCAAGCA AGGGGCCCTC GGCCTCTGCA TCTCCTGAGA ACTCCAGTGC AGGGGGGCCC 
AGTGGGAGCA GCAATGGCGC TGGCGAGAGC GGAGGGCAGG ACAGCACTTT CGAGTGCAAC 
ATCTGCTTGG ACACAGCCAA GGATGCCGTC ATCAGCCTGT GTGGCCACCT CTTCTGTTGG 
CCGTGTTTAC ATCAGGGATT TCAAGGATTT GGATTTGGAG ATGGTGGCTT CCAGATGTCT 
TTTGGAATTG GGGCATTTCC CTTTGGGATA TTTGCCACAG CATTTAATAT AAATGATGGG 
CGGCCTCCTC CAGCTGTCCC TGGGACACCC CAGTATGTGG ACGAGCAGTT CCTGTCACGC 
CTCTTCCTAT TTGTGGCCCT GGTGATCATG TTCTGGCTCC TGATTGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

The E3 ubiquitin ligase RNF185 facilitates the cGAS-mediated innate immune response.
PLoS pathogens13(3)e1006264(2017 Mar)
Wang Q,Huang L,Hong Z,Lv Z,Mao Z,Tang Y,Kong X,Li S,Cui Y,Liu H,Zhang L,Zhang X,Jiang L,Wang C,Zhou Q

Genome-wide identification and gene expression profiling of ubiquitin ligases for endoplasmic reticulum protein degradation.
Scientific reports630955(2016 Aug)
Kaneko M,Iwase I,Yamasaki Y,Takai T,Wu Y,Kanemoto S,Matsuhisa K,Asada R,Okuma Y,Watanabe T,Imaizumi K,Nomura Y

The E3 ligase RNF185 negatively regulates osteogenic differentiation by targeting Dvl2 for degradation.
Biochemical and biophysical research communications447(3)431-6(2014 May)
Zhou Y,Shang H,Zhang C,Liu Y,Zhao Y,Shuang F,Zhong H,Tang J,Hou S

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RNF185 cDNA ORF clone, Homo sapiens(Human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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