GADD45GIP1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GADD45GIP1
Entrez Gene ID	90480
Full Name	GADD45G interacting protein 1
Synonyms	CKBBP2,CKbetaBP2,CRIF1,MRP-L59,PLINP,PLINP-1,PRG6,Plinp1
General protein information	Preferred Names GADD45G interacting protein 1 Names growth arrest and DNA damage-inducible proteins-interacting protein 1 39S ribosomal protein L59, mitochondrial CKII beta binding protein 2 CKII beta-associating protein CR6 interacting factor 1 growth arrest and DNA-damage-inducible, gamma interacting protein 1 growth arrest- and DNA damage-inducible GADD45G-interacting protein mitochondrial large ribosomal subunit protein CRIF1 mitochondrial large ribosomal subunit protein mL64 p53-responsive gene 6 protein papillomavirus L2 interacting nuclear protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.13
Summary	This gene encodes a nuclear-localized protein that may be induced by p53 and regulates the cell cycle by inhibiting G1 to S phase progression. The encoded protein may interact with other cell cycle regulators. [provided by RefSeq, Aug 2012].
Disorder MIM:	605162

mRNA and Protein(s)

mRNA	Protein	Name
NM_052850.4	NP_443082.2	growth arrest and DNA damage-inducible proteins-interacting protein 1
NM_052850.3	NP_443082.2	growth arrest and DNA damage-inducible proteins-interacting protein 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GADD45GIP1	XP_533898.2
Bos taurus (cattle)	GADD45GIP1	NP_001073693.1
Mus musculus (house mouse)	Gadd45gip1	NP_899202.3
Danio rerio (zebrafish)	gadd45gip1	XP_001345133.3
Homo sapiens (human)	GADD45GIP1	NP_443082.2
Xenopus tropicalis (tropical clawed frog)	gadd45gip1	XP_002939166.1
Pan troglodytes (chimpanzee)	GADD45GIP1	XP_001170430.1
Rattus norvegicus (Norway rat)	Gadd45gip1	NP_001093974.1

Gene Ontology
Bibliography

Related articles in PubMed

CR6-Interacting Factor 1 Deficiency Impairs Vascular Function by Inhibiting the Sirt1-Endothelial Nitric Oxide Synthase Pathway.
Nagar H, Jung SB, Ryu MJ, Choi SJ, Piao S, Song HJ, Kang SK, Shin N, Kim DW, Jin SA, Jeong JO, Irani K, Jeon BH, Shong M, Kweon GR, Kim CS
Antioxidants & redox signaling27(4)234-249(2017 Aug)

CRIF1 enhances p53 activity via the chromatin remodeler SNF5 in the HCT116 colon cancer cell lines.
Yan HX, Zhang YJ, Zhang Y, Ren X, Shen YF, Cheng MB, Zhang Y
Biochimica et biophysica acta1860(4)516-522(2017 04)

Co-Activation of PKC-? by CRIF1 Modulates Oxidative Stress in Bone Marrow Multipotent Mesenchymal Stromal Cells after Irradiation by Phosphorylating NRF2 Ser40.
Chen L, Ran Q, Xiang Y, Xiang L, Chen L, Li F, Wu J, Wu C, Li Z
Theranostics7(10)2634-2648(2017)

Lymphocyte-specific protein tyrosine kinase (Lck) interacts with CR6-interacting factor 1 (CRIF1) in mitochondria to repress oxidative phosphorylation.
Vahedi S, Chueh FY, Chandran B, Yu CL
BMC cancer15551(2015 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SNF5 is indispensable for CRIF1-enhanced p53 activity and its function in the suppression of cell cycle arrest in human cancer cells.
Title: CRIF1 enhances p53 activity via the chromatin remodeler SNF5 in the HCT116 colon cancer cell lines.

Study shows that Lck interacts with CRIF1 in the mitochondria and negatively regulates CRIF1-mediated translation of mitochondrion-encoded proteins.
Title: Lymphocyte-specific protein tyrosine kinase (Lck) interacts with CR6-interacting factor 1 (CRIF1) in mitochondria to repress oxidative phosphorylation.

our results support a novel function of nuclear Lck in promoting human leukemic T cell survival through interaction with a tumor suppressor, CRIF1
Title: Nuclear lymphocyte-specific protein tyrosine kinase and its interaction with CR6-interacting factor 1 promote the survival of human leukemic T cells.

Crif1 is an indispensable regulator of PKAalpha cat that modulates the PKA/CREB signaling pathway to promote adipogenic differentiation of bone marrow mesenchymal stem cells after irradiation
Title: Crif1 Promotes Adipogenic Differentiation of Bone Marrow Mesenchymal Stem Cells After Irradiation by Modulating the PKA/CREB Signaling Pathway.

The results identify the ROS-Sp1-Crif1 pathway to be a new mechanism underlying Abeta-induced mitochondrial dysfunction and suggest that ROS-mediated downregulation of Crif1 is a crucial event in AD pathology.
Title: CR6-interacting factor 1 is a key regulator in A?-induced mitochondrial disruption and pathogenesis of Alzheimer's disease.

The following GADD45GIP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GADD45GIP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01788	NM_052850.4 Latest version!	Homo sapiens GADD45G interacting protein 1 (GADD45GIP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu01788	NM_052850.3	Homo sapiens GADD45G interacting protein 1 (GADD45GIP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GADD45GIP1 ( NM_052850.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_443082.2 Homo sapiens GADD45G interacting protein 1 (GADD45GIP1), mRNA.

Price & Availability↑

CloneID	OHu01788
Clone ID Related Accession (Same CDS sequence)	NM_052850.4 , NM_052850.3
Accession Version	NM_052850.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	growth arrest and DNA damage-inducible proteins-interacting protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF479749.1, AI309741.1 and AC092069.2. On Nov 23, 2018 this sequence version replaced NM_052850.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.1158843.1, SRR3476690.885567.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000316939.3/ ENSP00000323065.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCGT CCGTGCGACA GGCACGCAGC CTACTAGGTG TGGCGGCGAC CCTGGCCCCG 
GGTTCCCGTG GCTACCGGGC GCGGCCGCCC CCGCGCCGCA GGCCGGGACC CCGGTGGCCA 
GACCCCGAGG ACCTCCTGAC CCCGCGGTGG CAGCTGGGAC CGCGCTACGC GGCTAAGCAG 
TTCGCGCGTT ACGGCGCCGC CTCCGGGGTG GTCCCCGGTT CGTTATGGCC GTCGCCGGAG 
CAGCTGCGGG AGCTGGAGGC CGAAGAACGC GAATGGTACC CGAGCCTGGC GACCATGCAG 
GAGTCGCTGC GGGTGAAGCA GCTGGCCGAA GAGCAGAAGC GTCGGGAGAG GGAGCAGCAC 
ATCGCAGAGT GCATGGCCAA GATGCCACAG ATGATTGTGA ACTGGCAGCA GCAGCAGCGG 
GAGAACTGGG AGAAGGCCCA GGCTGACAAG GAGAGGAGGG CCCGACTGCA GGCTGAGGCC 
CAGGAGCTCC TGGGCTACCA GGTGGACCCA AGGAGTGCCC GCTTCCAGGA GCTGCTCCAG 
GACCTAGAGA AGAAGGAGCG CAAGCGCCTC AAGGAGGAAA AACAGAAACG GAAGAAGGAG 
GCGCGAGCTG CTGCATTGGC TGCAGCTGTG GCTCAAGACC CAGCAGCCTC TGGGGCACCC 
AGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_443082.2
CDS	12..680
Translation	MAASVRQARSLLGVAATLAPGSRGYRARPPPRRRPGPRWPDPEDLLTPRWQLGPRYAAKQFARYGAASGVVPGSLWPSPEQLRELEAEEREWYPSLATMQESLRVKQLAEEQKRREREQHIAECMAKMPQMIVNWQQQQRENWEKAQADKERRARLQAEAQELLGYQVDPRSARFQELLQDLEKKERKRLKEEKQKRKKEARAAALAAAVAQDPAASGAPSS

Target ORF information:

RefSeq Version	NM_052850.4
Organism	Homo sapiens(human)
Definition	Homo sapiens GADD45G interacting protein 1 (GADD45GIP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_052850.4

ORF Insert Sequence:

ATGGCGGCGT CCGTGCGACA GGCACGCAGC CTACTAGGTG TGGCGGCGAC CCTGGCCCCG 
GGTTCCCGTG GCTACCGGGC GCGGCCGCCC CCGCGCCGCA GGCCGGGACC CCGGTGGCCA 
GACCCCGAGG ACCTCCTGAC CCCGCGGTGG CAGCTGGGAC CGCGCTACGC GGCTAAGCAG 
TTCGCGCGTT ACGGCGCCGC CTCCGGGGTG GTCCCCGGTT CGTTATGGCC GTCGCCGGAG 
CAGCTGCGGG AGCTGGAGGC CGAAGAACGC GAATGGTACC CGAGCCTGGC GACCATGCAG 
GAGTCGCTGC GGGTGAAGCA GCTGGCCGAA GAGCAGAAGC GTCGGGAGAG GGAGCAGCAC 
ATCGCAGAGT GCATGGCCAA GATGCCACAG ATGATTGTGA ACTGGCAGCA GCAGCAGCGG 
GAGAACTGGG AGAAGGCCCA GGCTGACAAG GAGAGGAGGG CCCGACTGCA GGCTGAGGCC 
CAGGAGCTCC TGGGCTACCA GGTGGACCCA AGGAGTGCCC GCTTCCAGGA GCTGCTCCAG 
GACCTAGAGA AGAAGGAGCG CAAGCGCCTC AAGGAGGAAA AACAGAAACG GAAGAAGGAG 
GCGCGAGCTG CTGCATTGGC TGCAGCTGTG GCTCAAGACC CAGCAGCCTC TGGGGCACCC 
AGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GADD45GIP1 ( NM_052850.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_443082.2 Homo sapiens GADD45G interacting protein 1 (GADD45GIP1), mRNA.

Price & Availability↑

CloneID	OHu01788
Clone ID Related Accession (Same CDS sequence)	NM_052850.4 , NM_052850.3
Accession Version	NM_052850.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	growth arrest and DNA damage-inducible proteins-interacting protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI459923.1, AF479749.1 and AI309741.1. On Aug 4, 2012 this sequence version replaced NM_052850.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.505376.1, BI459923.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGT CCGTGCGACA GGCACGCAGC CTACTAGGTG TGGCGGCGAC CCTGGCCCCG 
GGTTCCCGTG GCTACCGGGC GCGGCCGCCC CCGCGCCGCA GGCCGGGACC CCGGTGGCCA 
GACCCCGAGG ACCTCCTGAC CCCGCGGTGG CAGCTGGGAC CGCGCTACGC GGCTAAGCAG 
TTCGCGCGTT ACGGCGCCGC CTCCGGGGTG GTCCCCGGTT CGTTATGGCC GTCGCCGGAG 
CAGCTGCGGG AGCTGGAGGC CGAAGAACGC GAATGGTACC CGAGCCTGGC GACCATGCAG 
GAGTCGCTGC GGGTGAAGCA GCTGGCCGAA GAGCAGAAGC GTCGGGAGAG GGAGCAGCAC 
ATCGCAGAGT GCATGGCCAA GATGCCACAG ATGATTGTGA ACTGGCAGCA GCAGCAGCGG 
GAGAACTGGG AGAAGGCCCA GGCTGACAAG GAGAGGAGGG CCCGACTGCA GGCTGAGGCC 
CAGGAGCTCC TGGGCTACCA GGTGGACCCA AGGAGTGCCC GCTTCCAGGA GCTGCTCCAG 
GACCTAGAGA AGAAGGAGCG CAAGCGCCTC AAGGAGGAAA AACAGAAACG GAAGAAGGAG 
GCGCGAGCTG CTGCATTGGC TGCAGCTGTG GCTCAAGACC CAGCAGCCTC TGGGGCACCC 
AGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_443082.2
CDS	43..711
Translation	MAASVRQARSLLGVAATLAPGSRGYRARPPPRRRPGPRWPDPEDLLTPRWQLGPRYAAKQFARYGAASGVVPGSLWPSPEQLRELEAEEREWYPSLATMQESLRVKQLAEEQKRREREQHIAECMAKMPQMIVNWQQQQRENWEKAQADKERRARLQAEAQELLGYQVDPRSARFQELLQDLEKKERKRLKEEKQKRKKEARAAALAAAVAQDPAASGAPSS

Target ORF information:

RefSeq Version	NM_052850.3
Organism	Homo sapiens(human)
Definition	Homo sapiens GADD45G interacting protein 1 (GADD45GIP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_052850.3

ORF Insert Sequence:

ATGGCGGCGT CCGTGCGACA GGCACGCAGC CTACTAGGTG TGGCGGCGAC CCTGGCCCCG 
GGTTCCCGTG GCTACCGGGC GCGGCCGCCC CCGCGCCGCA GGCCGGGACC CCGGTGGCCA 
GACCCCGAGG ACCTCCTGAC CCCGCGGTGG CAGCTGGGAC CGCGCTACGC GGCTAAGCAG 
TTCGCGCGTT ACGGCGCCGC CTCCGGGGTG GTCCCCGGTT CGTTATGGCC GTCGCCGGAG 
CAGCTGCGGG AGCTGGAGGC CGAAGAACGC GAATGGTACC CGAGCCTGGC GACCATGCAG 
GAGTCGCTGC GGGTGAAGCA GCTGGCCGAA GAGCAGAAGC GTCGGGAGAG GGAGCAGCAC 
ATCGCAGAGT GCATGGCCAA GATGCCACAG ATGATTGTGA ACTGGCAGCA GCAGCAGCGG 
GAGAACTGGG AGAAGGCCCA GGCTGACAAG GAGAGGAGGG CCCGACTGCA GGCTGAGGCC 
CAGGAGCTCC TGGGCTACCA GGTGGACCCA AGGAGTGCCC GCTTCCAGGA GCTGCTCCAG 
GACCTAGAGA AGAAGGAGCG CAAGCGCCTC AAGGAGGAAA AACAGAAACG GAAGAAGGAG 
GCGCGAGCTG CTGCATTGGC TGCAGCTGTG GCTCAAGACC CAGCAGCCTC TGGGGCACCC 
AGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CR6-Interacting Factor 1 Deficiency Impairs Vascular Function by Inhibiting the Sirt1-Endothelial Nitric Oxide Synthase Pathway.
Antioxidants & redox signaling27(4)234-249(2017 Aug)
Nagar H,Jung SB,Ryu MJ,Choi SJ,Piao S,Song HJ,Kang SK,Shin N,Kim DW,Jin SA,Jeong JO,i K,Jeon BH,Shong M,Kweon GR,Kim CS

CRIF1 enhances p53 activity via the chromatin remodeler SNF5 in the HCT116 colon cancer cell lines.
Biochimica et biophysica acta1860(4)516-522(2017 04)
Yan HX,Zhang YJ,Zhang Y,Ren X,Shen YF,Cheng MB,Zhang Y

Co-Activation of PKC-? by CRIF1 Modulates Oxidative Stress in Bone Marrow Multipotent Mesenchymal Stromal Cells after Irradiation by Phosphorylating NRF2 Ser40.
Theranostics7(10)2634-2648(2017)
Chen L,Ran Q,Xiang Y,Xiang L,Chen L,Li F,Wu J,Wu C,Li Z

Lymphocyte-specific protein tyrosine kinase (Lck) interacts with CR6-interacting factor 1 (CRIF1) in mitochondria to repress oxidative phosphorylation.
BMC cancer15551(2015 Jul)
Vahedi S,Chueh FY,Chandran B,Yu CL