RHNO1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RHNO1
Entrez Gene ID	83695
Full Name	RAD9-HUS1-RAD1 interacting nuclear orphan 1
Synonyms	C12orf32,HKMT1188,RHINO
General protein information	Preferred Names RAD9-HUS1-RAD1 interacting nuclear orphan 1 Names RAD9, HUS1, RAD1-interacting nuclear orphan protein 1 Rad9, Rad1, Hus1 interacting nuclear orphan rAD9, RAD1, HUS1-interacting nuclear orphan protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12p13.33
Disorder MIM:	614085

mRNA and Protein(s)

mRNA	Protein	Name
NM_001257098.2	NP_001244027.1	RAD9, HUS1, RAD1-interacting nuclear orphan protein 1 isoform 1
NM_001252500.3	NP_001239429.1	RAD9, HUS1, RAD1-interacting nuclear orphan protein 1 isoform 2
NM_001252499.2	NP_001239428.1	RAD9, HUS1, RAD1-interacting nuclear orphan protein 1 isoform 1
NM_001252500.2	NP_001239429.1	RAD9, HUS1, RAD1-interacting nuclear orphan protein 1 isoform 2
NM_001252499.3	NP_001239428.1	RAD9, HUS1, RAD1-interacting nuclear orphan protein 1 isoform 1
NM_001257098.1	NP_001244027.1	RAD9, HUS1, RAD1-interacting nuclear orphan protein 1 isoform 1
NM_001257097.1	NP_001244026.1	RAD9, HUS1, RAD1-interacting nuclear orphan protein 1 isoform 1

Pathways from BioSystems

Homologies

	LOC709008	XP_001098385.1
Canis lupus familiaris (dog)	RHNO1	XP_003433526.1
Xenopus tropicalis (tropical clawed frog)	rhno1	NP_001039069.1
Homo sapiens (human)	RHNO1	NP_001244027.1
Pan troglodytes (chimpanzee)	RHNO1	XP_508931.1
Bos taurus (cattle)	RHNO1	NP_001069099.1
Gallus gallus (chicken)	C12ORF32	NP_001006563.1

Gene Ontology
Bibliography

Related articles in PubMed

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

RHINO forms a stoichiometric complex with the 9-1-1 checkpoint clamp and mediates ATR-Chk1 signaling.
Lindsey-Boltz LA, Kemp MG, Capp C, Sancar A
Cell cycle (Georgetown, Tex.)14(1)99-108(2015)

Evaluation of the RHINO gene for breast cancer predisposition in Finnish breast cancer families.
Heikkinen T, Khan S, Huovari E, Vilske S, Schleutker J, Kallioniemi A, Blomqvist C, Aittom?ki K, Nevanlinna H
Breast cancer research and treatment144(2)437-41(2014 Apr)

A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling.
Cotta-Ramusino C, McDonald ER 3rd, Hurov K, Sowa ME, Harper JW, Elledge SJ
Science (New York, N.Y.)332(6035)1313-7(2011 Jun)

Involvement of C12orf32 overexpression in breast carcinogenesis.
Kim JW, Fukukawa C, Ueda K, Nishidate T, Katagiri T, Nakamura Y
International journal of oncology37(4)861-7(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knockdown of RHINO in human cells partially abrogated ATR-Chk1 kinase signaling following UV irradiation but did not impact the loading of Rad9-Hus1-Rad1 complex on chromatin or the association of Rad9-Hus1-Rad1 complex with TopBP1.
Title: RHINO forms a stoichiometric complex with the 9-1-1 checkpoint clamp and mediates ATR-Chk1 signaling.

Germline variation in the RHINO gene is unlikely to influence inherited susceptibility to breast cancer.
Title: Evaluation of the RHINO gene for breast cancer predisposition in Finnish breast cancer families.

We suggest that RHINO functions together with the 9-1-1 complex and TopBP1 to fully activate ATR (ataxia telangiectasia and Rad3-related.
Title: A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling.

Identified C12orf32 to be significantly up-regulated in the great majority of clinical breast cancer specimens.
Title: Involvement of C12orf32 overexpression in breast carcinogenesis.

The following RHNO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RHNO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02490	NM_001252499.3 Latest version!	Homo sapiens RAD9-HUS1-RAD1 interacting nuclear orphan 1 (RHNO1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu02490	NM_001257097.1 Latest version!	Homo sapiens RAD9-HUS1-RAD1 interacting nuclear orphan 1 (RHNO1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu02490	NM_001257098.2 Latest version!	Homo sapiens RAD9-HUS1-RAD1 interacting nuclear orphan 1 (RHNO1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu02491	NM_001252500.3 Latest version!	Homo sapiens RAD9-HUS1-RAD1 interacting nuclear orphan 1 (RHNO1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu02491	NM_001252500.2	Homo sapiens RAD9-HUS1-RAD1 interacting nuclear orphan 1 (RHNO1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu02490	NM_001252499.2	Homo sapiens RAD9-HUS1-RAD1 interacting nuclear orphan 1 (RHNO1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu02490	NM_001257098.1	Homo sapiens RAD9-HUS1-RAD1 interacting nuclear orphan 1 (RHNO1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RHNO1 ( NM_001257098.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001244027.1 Homo sapiens RAD9-HUS1-RAD1 interacting nuclear orphan 1 (RHNO1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu02490
Clone ID Related Accession (Same CDS sequence)	NM_001257098.2 , NM_001252499.2 , NM_001252499.3 , NM_001257098.1 , NM_001257097.1
Accession Version	NM_001257098.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	RAD9, HUS1, RAD1-interacting nuclear orphan protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA679606.1, AB073599.1 and AI631627.1. On Jun 1, 2019 this sequence version replaced NM_001257098.1. Transcript Variant: This variant (5) differs in the 5' UTR compared to variant 1. Variants 1, 4 and 5 all encode isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.752577.1, DA679606.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCCCA GAAAAAAACG CCGCCAGCCT TCCCAGAAAG CCCCGCTGCT GTTCCACCAA 
CAACCACTGG AGGGCCCCAA ACACAGCTGT GCATCTACAC AGCTTCCCAT CACTCACACT 
CGACAGGTGC CCAGCAAGCC CATTGACCAC AGCACCATCA CTTCCTGGGT ATCACCTGAT 
TTTGATACAG CAGCAGGAAG CTTGTTCCCA GCCTACCAGA AACACCAAAA CCGGGCGAGA 
CACTCAAGTC GAAAACCTAC CACCTCCAAG TTTCCACATC TAACTTTTGA GAGTCCGCAA 
TCTTCCAGTT CAGAGACATT GGGGATCCCC TTAATCCGAG AGTGCCCCAG TGAATCAGAA 
AAGGATGTTT CCAGAAGACC CTTAGTTCCA GTGCTCAGTC CCCAAAGCTG TGGGAACATG 
TCAGTGCAGG CACTTCAGAG CTTACCTTAT GTGTTCATTC CACCTGATAT CCAGACCCCA 
GAGTCATCGT CTGTGAAGGA AGAACTCATT CCCCAAGATC AGAAGGAAAA CAGCCTTCTA 
AGCTGCACTC TTCACACTGG CACTCCTAAT AGCCCAGAGC CTGGACCTGT TCTGGTTAAA 
GACACCCCCG AGGACAAGTA TGGAATAAAG GTCACATGGA GGAGACGACA GCACCTGCTT 
GCTTACCTCA GGGAGAGAGG GAAGCTGAGC AGAAGCCAAT TCCTTGTGAA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001244027.1
CDS	165..881
Translation	MPPRKKRRQPSQKAPLLFHQQPLEGPKHSCASTQLPITHTRQVPSKPIDHSTITSWVSPDFDTAAGSLFPAYQKHQNRARHSSRKPTTSKFPHLTFESPQSSSSETLGIPLIRECPSESEKDVSRRPLVPVLSPQSCGNMSVQALQSLPYVFIPPDIQTPESSSVKEELIPQDQKENSLLSCTLHTGTPNSPEPGPVLVKDTPEDKYGIKVTWRRRQHLLAYLRERGKLSRSQFLVKS

Target ORF information:

RefSeq Version	NM_001257098.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RAD9-HUS1-RAD1 interacting nuclear orphan 1 (RHNO1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001257098.2

ORF Insert Sequence:

ATGCCTCCCA GAAAAAAACG CCGCCAGCCT TCCCAGAAAG CCCCGCTGCT GTTCCACCAA 
CAACCACTGG AGGGCCCCAA ACACAGCTGT GCATCTACAC AGCTTCCCAT CACTCACACT 
CGACAGGTGC CCAGCAAGCC CATTGACCAC AGCACCATCA CTTCCTGGGT ATCACCTGAT 
TTTGATACAG CAGCAGGAAG CTTGTTCCCA GCCTACCAGA AACACCAAAA CCGGGCGAGA 
CACTCAAGTC GAAAACCTAC CACCTCCAAG TTTCCACATC TAACTTTTGA GAGTCCGCAA 
TCTTCCAGTT CAGAGACATT GGGGATCCCC TTAATCCGAG AGTGCCCCAG TGAATCAGAA 
AAGGATGTTT CCAGAAGACC CTTAGTTCCA GTGCTCAGTC CCCAAAGCTG TGGGAACATG 
TCAGTGCAGG CACTTCAGAG CTTACCTTAT GTGTTCATTC CACCTGATAT CCAGACCCCA 
GAGTCATCGT CTGTGAAGGA AGAACTCATT CCCCAAGATC AGAAGGAAAA CAGCCTTCTA 
AGCTGCACTC TTCACACTGG CACTCCTAAT AGCCCAGAGC CTGGACCTGT TCTGGTTAAA 
GACACCCCCG AGGACAAGTA TGGAATAAAG GTCACATGGA GGAGACGACA GCACCTGCTT 
GCTTACCTCA GGGAGAGAGG GAAGCTGAGC AGAAGCCAAT TCCTTGTGAA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHNO1 ( NM_001252499.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001239428.1 Homo sapiens RAD9-HUS1-RAD1 interacting nuclear orphan 1 (RHNO1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02490
Clone ID Related Accession (Same CDS sequence)	NM_001257098.2 , NM_001252499.2 , NM_001252499.3 , NM_001257098.1 , NM_001257097.1
Accession Version	NM_001252499.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	RAD9, HUS1, RAD1-interacting nuclear orphan protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA679606.1, AB073599.1 and AI631627.1. On or before Sep 18, 2012 this sequence version replaced NM_031465.2, NR_027363.1, NM_001252499.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 1, 4 and 5 all encode isoform 1. ##Evidence-Data-START## Transcript exon combination :: DA923751.1, SRR1803615.132148.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCCCA GAAAAAAACG CCGCCAGCCT TCCCAGAAAG CCCCGCTGCT GTTCCACCAA 
CAACCACTGG AGGGCCCCAA ACACAGCTGT GCATCTACAC AGCTTCCCAT CACTCACACT 
CGACAGGTGC CCAGCAAGCC CATTGACCAC AGCACCATCA CTTCCTGGGT ATCACCTGAT 
TTTGATACAG CAGCAGGAAG CTTGTTCCCA GCCTACCAGA AACACCAAAA CCGGGCGAGA 
CACTCAAGTC GAAAACCTAC CACCTCCAAG TTTCCACATC TAACTTTTGA GAGTCCGCAA 
TCTTCCAGTT CAGAGACATT GGGGATCCCC TTAATCCGAG AGTGCCCCAG TGAATCAGAA 
AAGGATGTTT CCAGAAGACC CTTAGTTCCA GTGCTCAGTC CCCAAAGCTG TGGGAACATG 
TCAGTGCAGG CACTTCAGAG CTTACCTTAT GTGTTCATTC CACCTGATAT CCAGACCCCA 
GAGTCATCGT CTGTGAAGGA AGAACTCATT CCCCAAGATC AGAAGGAAAA CAGCCTTCTA 
AGCTGCACTC TTCACACTGG CACTCCTAAT AGCCCAGAGC CTGGACCTGT TCTGGTTAAA 
GACACCCCCG AGGACAAGTA TGGAATAAAG GTCACATGGA GGAGACGACA GCACCTGCTT 
GCTTACCTCA GGGAGAGAGG GAAGCTGAGC AGAAGCCAAT TCCTTGTGAA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239428.1
CDS	169..885
Translation	MPPRKKRRQPSQKAPLLFHQQPLEGPKHSCASTQLPITHTRQVPSKPIDHSTITSWVSPDFDTAAGSLFPAYQKHQNRARHSSRKPTTSKFPHLTFESPQSSSSETLGIPLIRECPSESEKDVSRRPLVPVLSPQSCGNMSVQALQSLPYVFIPPDIQTPESSSVKEELIPQDQKENSLLSCTLHTGTPNSPEPGPVLVKDTPEDKYGIKVTWRRRQHLLAYLRERGKLSRSQFLVKS

Target ORF information:

RefSeq Version	NM_001252499.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RAD9-HUS1-RAD1 interacting nuclear orphan 1 (RHNO1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252499.2

ORF Insert Sequence:

ATGCCTCCCA GAAAAAAACG CCGCCAGCCT TCCCAGAAAG CCCCGCTGCT GTTCCACCAA 
CAACCACTGG AGGGCCCCAA ACACAGCTGT GCATCTACAC AGCTTCCCAT CACTCACACT 
CGACAGGTGC CCAGCAAGCC CATTGACCAC AGCACCATCA CTTCCTGGGT ATCACCTGAT 
TTTGATACAG CAGCAGGAAG CTTGTTCCCA GCCTACCAGA AACACCAAAA CCGGGCGAGA 
CACTCAAGTC GAAAACCTAC CACCTCCAAG TTTCCACATC TAACTTTTGA GAGTCCGCAA 
TCTTCCAGTT CAGAGACATT GGGGATCCCC TTAATCCGAG AGTGCCCCAG TGAATCAGAA 
AAGGATGTTT CCAGAAGACC CTTAGTTCCA GTGCTCAGTC CCCAAAGCTG TGGGAACATG 
TCAGTGCAGG CACTTCAGAG CTTACCTTAT GTGTTCATTC CACCTGATAT CCAGACCCCA 
GAGTCATCGT CTGTGAAGGA AGAACTCATT CCCCAAGATC AGAAGGAAAA CAGCCTTCTA 
AGCTGCACTC TTCACACTGG CACTCCTAAT AGCCCAGAGC CTGGACCTGT TCTGGTTAAA 
GACACCCCCG AGGACAAGTA TGGAATAAAG GTCACATGGA GGAGACGACA GCACCTGCTT 
GCTTACCTCA GGGAGAGAGG GAAGCTGAGC AGAAGCCAAT TCCTTGTGAA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHNO1 ( NM_001252499.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001239428.1 Homo sapiens RAD9-HUS1-RAD1 interacting nuclear orphan 1 (RHNO1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02490
Clone ID Related Accession (Same CDS sequence)	NM_001257098.2 , NM_001252499.2 , NM_001252499.3 , NM_001257098.1 , NM_001257097.1
Accession Version	NM_001252499.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	RAD9, HUS1, RAD1-interacting nuclear orphan protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB073599.1 and AI631627.1. On Nov 22, 2018 this sequence version replaced NM_001252499.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 1, 4 and 5 all encode isoform 1. ##Evidence-Data-START## Transcript exon combination :: AB073599.1, SRR1660807.139389.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000489288.7/ ENSP00000438590.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCTCCCA GAAAAAAACG CCGCCAGCCT TCCCAGAAAG CCCCGCTGCT GTTCCACCAA 
CAACCACTGG AGGGCCCCAA ACACAGCTGT GCATCTACAC AGCTTCCCAT CACTCACACT 
CGACAGGTGC CCAGCAAGCC CATTGACCAC AGCACCATCA CTTCCTGGGT ATCACCTGAT 
TTTGATACAG CAGCAGGAAG CTTGTTCCCA GCCTACCAGA AACACCAAAA CCGGGCGAGA 
CACTCAAGTC GAAAACCTAC CACCTCCAAG TTTCCACATC TAACTTTTGA GAGTCCGCAA 
TCTTCCAGTT CAGAGACATT GGGGATCCCC TTAATCCGAG AGTGCCCCAG TGAATCAGAA 
AAGGATGTTT CCAGAAGACC CTTAGTTCCA GTGCTCAGTC CCCAAAGCTG TGGGAACATG 
TCAGTGCAGG CACTTCAGAG CTTACCTTAT GTGTTCATTC CACCTGATAT CCAGACCCCA 
GAGTCATCGT CTGTGAAGGA AGAACTCATT CCCCAAGATC AGAAGGAAAA CAGCCTTCTA 
AGCTGCACTC TTCACACTGG CACTCCTAAT AGCCCAGAGC CTGGACCTGT TCTGGTTAAA 
GACACCCCCG AGGACAAGTA TGGAATAAAG GTCACATGGA GGAGACGACA GCACCTGCTT 
GCTTACCTCA GGGAGAGAGG GAAGCTGAGC AGAAGCCAAT TCCTTGTGAA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239428.1
CDS	145..861
Translation	MPPRKKRRQPSQKAPLLFHQQPLEGPKHSCASTQLPITHTRQVPSKPIDHSTITSWVSPDFDTAAGSLFPAYQKHQNRARHSSRKPTTSKFPHLTFESPQSSSSETLGIPLIRECPSESEKDVSRRPLVPVLSPQSCGNMSVQALQSLPYVFIPPDIQTPESSSVKEELIPQDQKENSLLSCTLHTGTPNSPEPGPVLVKDTPEDKYGIKVTWRRRQHLLAYLRERGKLSRSQFLVKS

Target ORF information:

RefSeq Version	NM_001252499.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RAD9-HUS1-RAD1 interacting nuclear orphan 1 (RHNO1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252499.3

ORF Insert Sequence:

ATGCCTCCCA GAAAAAAACG CCGCCAGCCT TCCCAGAAAG CCCCGCTGCT GTTCCACCAA 
CAACCACTGG AGGGCCCCAA ACACAGCTGT GCATCTACAC AGCTTCCCAT CACTCACACT 
CGACAGGTGC CCAGCAAGCC CATTGACCAC AGCACCATCA CTTCCTGGGT ATCACCTGAT 
TTTGATACAG CAGCAGGAAG CTTGTTCCCA GCCTACCAGA AACACCAAAA CCGGGCGAGA 
CACTCAAGTC GAAAACCTAC CACCTCCAAG TTTCCACATC TAACTTTTGA GAGTCCGCAA 
TCTTCCAGTT CAGAGACATT GGGGATCCCC TTAATCCGAG AGTGCCCCAG TGAATCAGAA 
AAGGATGTTT CCAGAAGACC CTTAGTTCCA GTGCTCAGTC CCCAAAGCTG TGGGAACATG 
TCAGTGCAGG CACTTCAGAG CTTACCTTAT GTGTTCATTC CACCTGATAT CCAGACCCCA 
GAGTCATCGT CTGTGAAGGA AGAACTCATT CCCCAAGATC AGAAGGAAAA CAGCCTTCTA 
AGCTGCACTC TTCACACTGG CACTCCTAAT AGCCCAGAGC CTGGACCTGT TCTGGTTAAA 
GACACCCCCG AGGACAAGTA TGGAATAAAG GTCACATGGA GGAGACGACA GCACCTGCTT 
GCTTACCTCA GGGAGAGAGG GAAGCTGAGC AGAAGCCAAT TCCTTGTGAA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHNO1 ( NM_001257098.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001244027.1 Homo sapiens RAD9-HUS1-RAD1 interacting nuclear orphan 1 (RHNO1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu02490
Clone ID Related Accession (Same CDS sequence)	NM_001257098.2 , NM_001252499.2 , NM_001252499.3 , NM_001257098.1 , NM_001257097.1
Accession Version	NM_001257098.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-10
Organism	Homo sapiens(human)
Product	RAD9, HUS1, RAD1-interacting nuclear orphan protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA679606.1, AB073599.1 and AI631627.1. Transcript Variant: This variant (5) differs in the 5' UTR compared to variant 1. Variants 1, 4 and 5 all encode isoform 1. ##Evidence-Data-START## Transcript exon combination :: DA679606.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCCCA GAAAAAAACG CCGCCAGCCT TCCCAGAAAG CCCCGCTGCT GTTCCACCAA 
CAACCACTGG AGGGCCCCAA ACACAGCTGT GCATCTACAC AGCTTCCCAT CACTCACACT 
CGACAGGTGC CCAGCAAGCC CATTGACCAC AGCACCATCA CTTCCTGGGT ATCACCTGAT 
TTTGATACAG CAGCAGGAAG CTTGTTCCCA GCCTACCAGA AACACCAAAA CCGGGCGAGA 
CACTCAAGTC GAAAACCTAC CACCTCCAAG TTTCCACATC TAACTTTTGA GAGTCCGCAA 
TCTTCCAGTT CAGAGACATT GGGGATCCCC TTAATCCGAG AGTGCCCCAG TGAATCAGAA 
AAGGATGTTT CCAGAAGACC CTTAGTTCCA GTGCTCAGTC CCCAAAGCTG TGGGAACATG 
TCAGTGCAGG CACTTCAGAG CTTACCTTAT GTGTTCATTC CACCTGATAT CCAGACCCCA 
GAGTCATCGT CTGTGAAGGA AGAACTCATT CCCCAAGATC AGAAGGAAAA CAGCCTTCTA 
AGCTGCACTC TTCACACTGG CACTCCTAAT AGCCCAGAGC CTGGACCTGT TCTGGTTAAA 
GACACCCCCG AGGACAAGTA TGGAATAAAG GTCACATGGA GGAGACGACA GCACCTGCTT 
GCTTACCTCA GGGAGAGAGG GAAGCTGAGC AGAAGCCAAT TCCTTGTGAA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001244027.1
CDS	189..905
Translation	MPPRKKRRQPSQKAPLLFHQQPLEGPKHSCASTQLPITHTRQVPSKPIDHSTITSWVSPDFDTAAGSLFPAYQKHQNRARHSSRKPTTSKFPHLTFESPQSSSSETLGIPLIRECPSESEKDVSRRPLVPVLSPQSCGNMSVQALQSLPYVFIPPDIQTPESSSVKEELIPQDQKENSLLSCTLHTGTPNSPEPGPVLVKDTPEDKYGIKVTWRRRQHLLAYLRERGKLSRSQFLVKS

Target ORF information:

RefSeq Version	NM_001257098.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RAD9-HUS1-RAD1 interacting nuclear orphan 1 (RHNO1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001257098.1

ORF Insert Sequence:

ATGCCTCCCA GAAAAAAACG CCGCCAGCCT TCCCAGAAAG CCCCGCTGCT GTTCCACCAA 
CAACCACTGG AGGGCCCCAA ACACAGCTGT GCATCTACAC AGCTTCCCAT CACTCACACT 
CGACAGGTGC CCAGCAAGCC CATTGACCAC AGCACCATCA CTTCCTGGGT ATCACCTGAT 
TTTGATACAG CAGCAGGAAG CTTGTTCCCA GCCTACCAGA AACACCAAAA CCGGGCGAGA 
CACTCAAGTC GAAAACCTAC CACCTCCAAG TTTCCACATC TAACTTTTGA GAGTCCGCAA 
TCTTCCAGTT CAGAGACATT GGGGATCCCC TTAATCCGAG AGTGCCCCAG TGAATCAGAA 
AAGGATGTTT CCAGAAGACC CTTAGTTCCA GTGCTCAGTC CCCAAAGCTG TGGGAACATG 
TCAGTGCAGG CACTTCAGAG CTTACCTTAT GTGTTCATTC CACCTGATAT CCAGACCCCA 
GAGTCATCGT CTGTGAAGGA AGAACTCATT CCCCAAGATC AGAAGGAAAA CAGCCTTCTA 
AGCTGCACTC TTCACACTGG CACTCCTAAT AGCCCAGAGC CTGGACCTGT TCTGGTTAAA 
GACACCCCCG AGGACAAGTA TGGAATAAAG GTCACATGGA GGAGACGACA GCACCTGCTT 
GCTTACCTCA GGGAGAGAGG GAAGCTGAGC AGAAGCCAAT TCCTTGTGAA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHNO1 ( NM_001257097.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001244026.1 Homo sapiens RAD9-HUS1-RAD1 interacting nuclear orphan 1 (RHNO1), transcript variant 4, mRNA.

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CloneID	OHu02490
Clone ID Related Accession (Same CDS sequence)	NM_001257098.2 , NM_001252499.2 , NM_001252499.3 , NM_001257098.1 , NM_001257097.1
Accession Version	NM_001257097.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	RAD9, HUS1, RAD1-interacting nuclear orphan protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA679606.1, DA749974.1, AB073599.1 and AI631627.1. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1, 4 and 5 all encode isoform 1. ##Evidence-Data-START## Transcript exon combination :: DA749974.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCCCA GAAAAAAACG CCGCCAGCCT TCCCAGAAAG CCCCGCTGCT GTTCCACCAA 
CAACCACTGG AGGGCCCCAA ACACAGCTGT GCATCTACAC AGCTTCCCAT CACTCACACT 
CGACAGGTGC CCAGCAAGCC CATTGACCAC AGCACCATCA CTTCCTGGGT ATCACCTGAT 
TTTGATACAG CAGCAGGAAG CTTGTTCCCA GCCTACCAGA AACACCAAAA CCGGGCGAGA 
CACTCAAGTC GAAAACCTAC CACCTCCAAG TTTCCACATC TAACTTTTGA GAGTCCGCAA 
TCTTCCAGTT CAGAGACATT GGGGATCCCC TTAATCCGAG AGTGCCCCAG TGAATCAGAA 
AAGGATGTTT CCAGAAGACC CTTAGTTCCA GTGCTCAGTC CCCAAAGCTG TGGGAACATG 
TCAGTGCAGG CACTTCAGAG CTTACCTTAT GTGTTCATTC CACCTGATAT CCAGACCCCA 
GAGTCATCGT CTGTGAAGGA AGAACTCATT CCCCAAGATC AGAAGGAAAA CAGCCTTCTA 
AGCTGCACTC TTCACACTGG CACTCCTAAT AGCCCAGAGC CTGGACCTGT TCTGGTTAAA 
GACACCCCCG AGGACAAGTA TGGAATAAAG GTCACATGGA GGAGACGACA GCACCTGCTT 
GCTTACCTCA GGGAGAGAGG GAAGCTGAGC AGAAGCCAAT TCCTTGTGAA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001244026.1
CDS	290..1006
Translation	MPPRKKRRQPSQKAPLLFHQQPLEGPKHSCASTQLPITHTRQVPSKPIDHSTITSWVSPDFDTAAGSLFPAYQKHQNRARHSSRKPTTSKFPHLTFESPQSSSSETLGIPLIRECPSESEKDVSRRPLVPVLSPQSCGNMSVQALQSLPYVFIPPDIQTPESSSVKEELIPQDQKENSLLSCTLHTGTPNSPEPGPVLVKDTPEDKYGIKVTWRRRQHLLAYLRERGKLSRSQFLVKS

Target ORF information:

RefSeq Version	NM_001257097.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RAD9-HUS1-RAD1 interacting nuclear orphan 1 (RHNO1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001257097.1

ORF Insert Sequence:

ATGCCTCCCA GAAAAAAACG CCGCCAGCCT TCCCAGAAAG CCCCGCTGCT GTTCCACCAA 
CAACCACTGG AGGGCCCCAA ACACAGCTGT GCATCTACAC AGCTTCCCAT CACTCACACT 
CGACAGGTGC CCAGCAAGCC CATTGACCAC AGCACCATCA CTTCCTGGGT ATCACCTGAT 
TTTGATACAG CAGCAGGAAG CTTGTTCCCA GCCTACCAGA AACACCAAAA CCGGGCGAGA 
CACTCAAGTC GAAAACCTAC CACCTCCAAG TTTCCACATC TAACTTTTGA GAGTCCGCAA 
TCTTCCAGTT CAGAGACATT GGGGATCCCC TTAATCCGAG AGTGCCCCAG TGAATCAGAA 
AAGGATGTTT CCAGAAGACC CTTAGTTCCA GTGCTCAGTC CCCAAAGCTG TGGGAACATG 
TCAGTGCAGG CACTTCAGAG CTTACCTTAT GTGTTCATTC CACCTGATAT CCAGACCCCA 
GAGTCATCGT CTGTGAAGGA AGAACTCATT CCCCAAGATC AGAAGGAAAA CAGCCTTCTA 
AGCTGCACTC TTCACACTGG CACTCCTAAT AGCCCAGAGC CTGGACCTGT TCTGGTTAAA 
GACACCCCCG AGGACAAGTA TGGAATAAAG GTCACATGGA GGAGACGACA GCACCTGCTT 
GCTTACCTCA GGGAGAGAGG GAAGCTGAGC AGAAGCCAAT TCCTTGTGAA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHNO1 ( NM_001252500.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001239429.1 Homo sapiens RAD9-HUS1-RAD1 interacting nuclear orphan 1 (RHNO1), transcript variant 2, mRNA.

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CloneID	OHu02491
Clone ID Related Accession (Same CDS sequence)	NM_001252500.3 , NM_001252500.2
Accession Version	NM_001252500.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	RAD9, HUS1, RAD1-interacting nuclear orphan protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK304613.1, AB073599.1 and AI631627.1. On May 31, 2019 this sequence version replaced NM_001252500.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in a shorter isoform (2), compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK304613.1, SRR3476690.780397.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCCCA GAAAAAAACG CCGCCAGCCT TCCCAGAAAG CCCCGCTGCT GTTCCACCAA 
CAACCACTGG AGGGCCCCAA ACACAGCTGT GCATCTACAC AGCTTCCCAT CACTCACACT 
CGACAGGTAT CACCTGATTT TGATACAGCA GCAGGAAGCT TGTTCCCAGC CTACCAGAAA 
CACCAAAACC GGGCGAGACA CTCAAGTCGA AAACCTACCA CCTCCAAGTT TCCACATCTA 
ACTTTTGAGA GTCCGCAATC TTCCAGTTCA GAGACATTGG GGATCCCCTT AATCCGAGAG 
TGCCCCAGTG AATCAGAAAA GGATGTTTCC AGAAGACCCT TAGTTCCAGT GCTCAGTCCC 
CAAAGCTGTG GGAACATGTC AGTGCAGGCA CTTCAGAGCT TACCTTATGT GTTCATTCCA 
CCTGATATCC AGACCCCAGA GTCATCGTCT GTGAAGGAAG AACTCATTCC CCAAGATCAG 
AAGGAAAACA GCCTTCTAAG CTGCACTCTT CACACTGGCA CTCCTAATAG CCCAGAGCCT 
GGACCTGTTC TGGTTAAAGA CACCCCCGAG GACAAGTATG GAATAAAGGT CACATGGAGG 
AGACGACAGC ACCTGCTTGC TTACCTCAGG GAGAGAGGGA AGCTGAGCAG AAGCCAATTC 
CTTGTGAAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239429.1
CDS	145..819
Translation	MPPRKKRRQPSQKAPLLFHQQPLEGPKHSCASTQLPITHTRQVSPDFDTAAGSLFPAYQKHQNRARHSSRKPTTSKFPHLTFESPQSSSSETLGIPLIRECPSESEKDVSRRPLVPVLSPQSCGNMSVQALQSLPYVFIPPDIQTPESSSVKEELIPQDQKENSLLSCTLHTGTPNSPEPGPVLVKDTPEDKYGIKVTWRRRQHLLAYLRERGKLSRSQFLVKS

Target ORF information:

RefSeq Version	NM_001252500.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RAD9-HUS1-RAD1 interacting nuclear orphan 1 (RHNO1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252500.3

ORF Insert Sequence:

ATGCCTCCCA GAAAAAAACG CCGCCAGCCT TCCCAGAAAG CCCCGCTGCT GTTCCACCAA 
CAACCACTGG AGGGCCCCAA ACACAGCTGT GCATCTACAC AGCTTCCCAT CACTCACACT 
CGACAGGTAT CACCTGATTT TGATACAGCA GCAGGAAGCT TGTTCCCAGC CTACCAGAAA 
CACCAAAACC GGGCGAGACA CTCAAGTCGA AAACCTACCA CCTCCAAGTT TCCACATCTA 
ACTTTTGAGA GTCCGCAATC TTCCAGTTCA GAGACATTGG GGATCCCCTT AATCCGAGAG 
TGCCCCAGTG AATCAGAAAA GGATGTTTCC AGAAGACCCT TAGTTCCAGT GCTCAGTCCC 
CAAAGCTGTG GGAACATGTC AGTGCAGGCA CTTCAGAGCT TACCTTATGT GTTCATTCCA 
CCTGATATCC AGACCCCAGA GTCATCGTCT GTGAAGGAAG AACTCATTCC CCAAGATCAG 
AAGGAAAACA GCCTTCTAAG CTGCACTCTT CACACTGGCA CTCCTAATAG CCCAGAGCCT 
GGACCTGTTC TGGTTAAAGA CACCCCCGAG GACAAGTATG GAATAAAGGT CACATGGAGG 
AGACGACAGC ACCTGCTTGC TTACCTCAGG GAGAGAGGGA AGCTGAGCAG AAGCCAATTC 
CTTGTGAAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHNO1 ( NM_001252500.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001239429.1 Homo sapiens RAD9-HUS1-RAD1 interacting nuclear orphan 1 (RHNO1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02491
Clone ID Related Accession (Same CDS sequence)	NM_001252500.3 , NM_001252500.2
Accession Version	NM_001252500.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	RAD9, HUS1, RAD1-interacting nuclear orphan protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA679606.1, AK304613.1, AB073599.1 and AI631627.1. On or before Mar 12, 2012 this sequence version replaced NR_027364.1, NM_001252500.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in a shorter isoform (2), compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.181528.1, AK304613.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCCCA GAAAAAAACG CCGCCAGCCT TCCCAGAAAG CCCCGCTGCT GTTCCACCAA 
CAACCACTGG AGGGCCCCAA ACACAGCTGT GCATCTACAC AGCTTCCCAT CACTCACACT 
CGACAGGTAT CACCTGATTT TGATACAGCA GCAGGAAGCT TGTTCCCAGC CTACCAGAAA 
CACCAAAACC GGGCGAGACA CTCAAGTCGA AAACCTACCA CCTCCAAGTT TCCACATCTA 
ACTTTTGAGA GTCCGCAATC TTCCAGTTCA GAGACATTGG GGATCCCCTT AATCCGAGAG 
TGCCCCAGTG AATCAGAAAA GGATGTTTCC AGAAGACCCT TAGTTCCAGT GCTCAGTCCC 
CAAAGCTGTG GGAACATGTC AGTGCAGGCA CTTCAGAGCT TACCTTATGT GTTCATTCCA 
CCTGATATCC AGACCCCAGA GTCATCGTCT GTGAAGGAAG AACTCATTCC CCAAGATCAG 
AAGGAAAACA GCCTTCTAAG CTGCACTCTT CACACTGGCA CTCCTAATAG CCCAGAGCCT 
GGACCTGTTC TGGTTAAAGA CACCCCCGAG GACAAGTATG GAATAAAGGT CACATGGAGG 
AGACGACAGC ACCTGCTTGC TTACCTCAGG GAGAGAGGGA AGCTGAGCAG AAGCCAATTC 
CTTGTGAAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239429.1
CDS	169..843
Translation	MPPRKKRRQPSQKAPLLFHQQPLEGPKHSCASTQLPITHTRQVSPDFDTAAGSLFPAYQKHQNRARHSSRKPTTSKFPHLTFESPQSSSSETLGIPLIRECPSESEKDVSRRPLVPVLSPQSCGNMSVQALQSLPYVFIPPDIQTPESSSVKEELIPQDQKENSLLSCTLHTGTPNSPEPGPVLVKDTPEDKYGIKVTWRRRQHLLAYLRERGKLSRSQFLVKS

Target ORF information:

RefSeq Version	NM_001252500.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RAD9-HUS1-RAD1 interacting nuclear orphan 1 (RHNO1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252500.2

ORF Insert Sequence:

ATGCCTCCCA GAAAAAAACG CCGCCAGCCT TCCCAGAAAG CCCCGCTGCT GTTCCACCAA 
CAACCACTGG AGGGCCCCAA ACACAGCTGT GCATCTACAC AGCTTCCCAT CACTCACACT 
CGACAGGTAT CACCTGATTT TGATACAGCA GCAGGAAGCT TGTTCCCAGC CTACCAGAAA 
CACCAAAACC GGGCGAGACA CTCAAGTCGA AAACCTACCA CCTCCAAGTT TCCACATCTA 
ACTTTTGAGA GTCCGCAATC TTCCAGTTCA GAGACATTGG GGATCCCCTT AATCCGAGAG 
TGCCCCAGTG AATCAGAAAA GGATGTTTCC AGAAGACCCT TAGTTCCAGT GCTCAGTCCC 
CAAAGCTGTG GGAACATGTC AGTGCAGGCA CTTCAGAGCT TACCTTATGT GTTCATTCCA 
CCTGATATCC AGACCCCAGA GTCATCGTCT GTGAAGGAAG AACTCATTCC CCAAGATCAG 
AAGGAAAACA GCCTTCTAAG CTGCACTCTT CACACTGGCA CTCCTAATAG CCCAGAGCCT 
GGACCTGTTC TGGTTAAAGA CACCCCCGAG GACAAGTATG GAATAAAGGT CACATGGAGG 
AGACGACAGC ACCTGCTTGC TTACCTCAGG GAGAGAGGGA AGCTGAGCAG AAGCCAATTC 
CTTGTGAAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP

RHINO forms a stoichiometric complex with the 9-1-1 checkpoint clamp and mediates ATR-Chk1 signaling.
Cell cycle (Georgetown, Tex.)14(1)99-108(2015)
Lindsey-Boltz LA,Kemp MG,Capp C,Sancar A

Evaluation of the RHINO gene for breast cancer predisposition in Finnish breast cancer families.
Breast cancer research and treatment144(2)437-41(2014 Apr)
Heikkinen T,Khan S,Huovari E,Vilske S,Schleutker J,Kallioniemi A,Blomqvist C,Aittom?ki K,Nevanlinna H

A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling.
Science (New York, N.Y.)332(6035)1313-7(2011 Jun)
Cotta-Ramusino C,McDonald ER 3rd,Hurov K,Sowa ME,Harper JW,Elledge SJ

Involvement of C12orf32 overexpression in breast carcinogenesis.
International journal of oncology37(4)861-7(2010 Oct)
Kim JW,Fukukawa C,Ueda K,Nishidate T,Katagiri T,Nakamura Y

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RHNO1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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