FRAS1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FRAS1
Entrez Gene ID	80144
Full Name	Fraser extracellular matrix complex subunit 1
Synonyms	FRASRS1
General protein information	Preferred Names Fraser extracellular matrix complex subunit 1 Names extracellular matrix protein FRAS1 Fraser syndrome 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q21.21
Summary	This gene encodes an extracellular matrix protein that appears to function in the regulation of epidermal-basement membrane adhesion and organogenesis during development. Mutations in this gene cause Fraser syndrome, a multisystem malformation that can include craniofacial, urogenital and respiratory system abnormalities. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009].
Disorder MIM:	607830
Disorder Html:	Fraser syndrome 1, 219000 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001166133.1	NP_001159605.1	extracellular matrix protein FRAS1 isoform 2 precursor
XM_006714316.3	XP_006714379.1	extracellular matrix protein FRAS1 isoform X1
XM_006714316.2	XP_006714379.1	extracellular matrix protein FRAS1 isoform X1
NM_025074.7	NP_079350.5	extracellular matrix protein FRAS1 isoform 1 precursor
NM_025074.6	NP_079350.5	extracellular matrix protein FRAS1 isoform 1 precursor

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	fras1	XP_002935497.2
Gallus gallus (chicken)	FRAS1	XP_420470.4
Danio rerio (zebrafish)	fras1	NP_001124312.1
Homo sapiens (human)	FRAS1	NP_079350.5
Pan troglodytes (chimpanzee)	FRAS1	XP_517202.2
Mus musculus (house mouse)	Fras1	NP_780682.3
Canis lupus familiaris (dog)	FRAS1	XP_005639157.1
Rattus norvegicus (Norway rat)	Fras1	NP_001178524.1

Gene Ontology
Bibliography

Related articles in PubMed

Mild recessive mutations in six Fraser syndrome-related genes cause isolated congenital anomalies of the kidney and urinary tract.
Kohl S, Hwang DY, Dworschak GC, Hilger AC, Saisawat P, Vivante A, Stajic N, Bogdanovic R, Reutter HM, Kehinde EO, Tasic V, Hildebrandt F
Journal of the American Society of Nephrology : JASN25(9)1917-22(2014 Sep)

A genome wide association study of plasma uric acid levels in obese cases and never-overweight controls.
Li WD, Jiao H, Wang K, Zhang CK, Glessner JT, Grant SF, Zhao H, Hakonarson H, Arlen Price R
Obesity (Silver Spring, Md.)21(9)E490-4(2013 Sep)

Expanding the mutation spectrum for Fraser syndrome: identification of a novel heterozygous deletion in FRAS1.
Hoefele J, Wilhelm C, Schiesser M, Mack R, Heinrich U, Weber LT, Biskup S, Daumer-Haas C, Klein HG, Rost I
Gene520(2)194-7(2013 May)

A puzzle over several decades: eye anomalies with FRAS1 and STRA6 mutations in the same family.
Ng WY, Pasutto F, Bardakjian TM, Wilson MJ, Watson G, Schneider A, Mackey DA, Grigg JR, Zenker M, Jamieson RV
Clinical genetics83(2)162-8(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In 15 of 590 families, we identified recessive mutations in the genes FRAS1, FREM2, GRIP1, FREM1, ITGA8, and GREM1, all of which function in the interaction of the ureteric bud and the metanephric mesenchyme.
Title: Mild recessive mutations in six Fraser syndrome-related genes cause isolated congenital anomalies of the kidney and urinary tract.

First case of a family with two patients affected by Fraser syndrome due to a deletion of 64 kb (deletion 4q21.21) and an additional novel frameshift mutation in exon 66 of the FRAS1 gene.
Title: Expanding the mutation spectrum for Fraser syndrome: identification of a novel heterozygous deletion in FRAS1.

Analysis of FRAS1 and STRA6 mutations in the same family with eye anomalies.
Title: A puzzle over several decades: eye anomalies with FRAS1 and STRA6 mutations in the same family.

Heterozygous missense mutations in FRAS1 cause non-syndromic congenital abnormalities of the kidney and urinary tract in humans.
Title: Identification of two novel CAKUT-causing genes by massively parallel exon resequencing of candidate genes in patients with unilateral renal agenesis.

molecular, clinical findings of 4 fetuses with Fraser syndrome from 2 families; in family one, found nonsense mutation (c.3730C>T, p.R1244X) previously described; in family 2 found a novel nonsense mutation previously not known (c.370C>T, p.R124X)
Title: Clinical and molecular studies in two families with Fraser syndrome: a new FRAS1 gene mutation, prenatal ultrasound findings and implications for genetic counselling.

The following FRAS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FRAS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28520	NM_001166133.1 Latest version!	Homo sapiens Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	Quote Price
OHu28517	NM_025074.7 Latest version!	Homo sapiens Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu39089	XM_006714316.3 Latest version!	Homo sapiens Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OHu39089	XM_006714316.2	Homo sapiens Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu28517	NM_025074.6	Homo sapiens Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FRAS1 ( NM_001166133.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159605.1 Homo sapiens Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28520
Clone ID Related Accession (Same CDS sequence)	NM_001166133.1
Accession Version	NM_001166133.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Homo sapiens(human)
Product	extracellular matrix protein FRAS1 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA725113.1, DA881824.1, BC131820.1, AC093886.2 and AK025684.1. Transcript Variant: This variant (2) differs in the presence and absence of exons in its 3' coding region and 3' UTR, compared to variant 1, resulting in an isoform (2) with a distinct and significantly shorter C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC131820.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTGTCC TCAAAGTGTG GCTCGGGCTG GCCCTAGCGT TGGCGGAATT TGCAGTATTG 
CCTCATCATT CCGAAGGTGC TTGTGTCTAT CAGGATTCCT TGTTGGCGGA TGCCACAATT 
TGGAAGCCCG ATTCATGCCA GAGCTGCCGT TGCCATGGTG ATATTGTTAT CTGCAAACCT 
GCTGTTTGCA GAAACCCTCA ATGTGCCTTT GAGAAGGGAG AAGTGCTTCA AATAGCTGCC 
AACCAATGCT GTCCTGAGTG TGTTTTGAGG ACTCCAGGAT CTTGCCATCA TGAAAAGAAA 
ATCCATGAGC ATGGGACAGA ATGGGCCTCT TCTCCATGTA GTGTGTGCTC TTGCAATCAT 
GGGGAAGTCC GATGTACCCC CCAACCATGC CCACCGCTGT CATGTGGACA CCAGGAGCTG 
GCATTCATCC CTGAAGGAAG CTGCTGCCCA GTTTGTGTGG GCCTTGGGAA ACCCTGTTCC 
TATGAAGGCC ATGTGTTTCA GGATGGGGAG GACTGGCGGC TGAGCCGGTG TGCCAAATGT 
CTGTGTAGAA ATGGGGTTGC CCAGTGCTTC ACAGCTCAGT GTCAGCCTCT ATTTTGTAAC 
CAGGATGAGA CTGTAGTCCG AGTCCCTGGA AAATGTTGCC CGCAGTGCTC TGCAAGATCC 
TGCTCTGCAG CTGGCCAAGT ATACGAGCAT GGTGAGCAGT GGAGCGAAAA TGCCTGCACC 
ACGTGTATAT GTGACCGGGG TGAGGTCAGG TGTCACAAGC AGGCCTGCCT GCCCCTGAGA 
TGCGGAAAGG GTCAGAGCAG GGCTCGGCGT CATGGGCAAT GCTGTGAGGA ATGTGTGTCT 
CCTGCCGGGA GCTGCTCCTA TGATGGAGTT GTGCGGTACC AGGACGAAAT GTGGAAGGGC 
TCGGCCTGTG AGTTCTGCAT GTGTGATCAT GGCCAAGTGA CCTGCCAGAC TGGAGAGTGT 
GCCAAAGTGG AGTGTGCCCG GGATGAAGAA TTAATTCACT TAGATGGAAA GTGTTGTCCT 
GAATGCATTT CAAGGAATGG TTATTGTGTT TATGAAGAAA CTGGAGAATT TATGTCATCA 
AATGCTAGTG AAGTTAAACG TATTCCAGAG GGAGAGAAGT GGGAAGATGG CCCTTGCAAG 
GTGTGTGAGT GCCGAGGGGC TCAGGTAACT TGCTACGAGC CCTCTTGCCC ACCATGTCCA 
GTGGGCACAC TGGCCTTAGA GGTGAAGGGA CAGTGCTGTC CAGACTGCAC ATCAGTTCAT 
TGCCATCCAG ATTGTTTGAC ATGCTCTCAG TCTCCAGACC ACTGTGACCT CTGCCAAGAT 
CCTACCAAGT TACTGCAGAA TGGATGGTGT GTGCACAGCT GTGGACTGGG TTTTTACCAA 
GCTGGCAGTC TCTGTTTAGC CTGCCAGCCC CAGTGCTCCA CGTGTACCAG TGGGCTGGAG 
TGCTCATCCT GCCAGCCTCC CCTGCTGATG CGGCACGGGC AGTGTGTGCC TACCTGTGGG 
GACGGCTTCT ACCAAGATCG CCATTCCTGT GCAGTCTGCC ATGAGTCCTG TGCAGGTTGC 
TGGGGCCCAA CGGAGAAGCA CTGCTTGGCC TGCAGAGATC CCCTCCACGT GCTGAGAGAT 
GGCGGCTGTG AGAGCAGCTG TGGAAAAGGC TTCTACAACA GGCAGGGCAC CTGTAGCGCT 
TGTGACCAAT CCTGTGACAG TTGTGGCCCC AGTAGCCCCA GGTGTCTTAC CTGTACTGAG 
AAGACAGTGC TGCATGATGG GAAATGCATG TCTGAATGCC CTGGCGGGTA CTATGCTGAT 
GCCACTGGCA GGTGCAAAGT TTGTCATAAC TCATGTGCCA GCTGCTCTGG GCCCACACCC 
TCTCACTGTA CAGCCTGCAG CCCCCCCAAG GCTCTGCGTC AAGGCCACTG TCTGCCCCGC 
TGTGGAGAGG GTTTCTACTC TGACCATGGA GTCTGCAAAG CCTGTCACTC CTCCTGCCTG 
GCTTGTATGG GTCCCGCACC CTCTCACTGT ACTGGGTGTA AGAAGCCAGA GGAAGGACTG 
CAAGTGGAGC AGCTGTCTGA CGTGGGCATC CCCTCTGGCG AGTGTCTAGC CCAGTGTAGA 
GCCCATTTTT ACTTGGAGAG CACTGGCATA TGTGAAGCTT GCCACCAGTC CTGTTTCAGA 
TGTGCAGGGA AAAGCCCACA TAACTGCACA GACTGTGGGC CTTCCCATGT GCTGTTGGAT 
GGGCAGTGCC TCTCCCAGTG CCCAGATGGC TACTTTCACC AGGAAGGTAG TTGCACAGAG 
TGTCACCCAA CCTGCAGGCA GTGTCATGGG CCGTTGGAGT CTGACTGCAT CTCCTGTTAC 
CCTCACATCT CTCTTACCAA TGGTAACTGC AGGACCAGCT GCAGGGAAGA GCAGTTCCTC 
AACCTCGTGG GATACTGTGC TGACTGCCAT CACCTGTGCC AGCACTGTGC AGCTGATCTC 
CACAACACTG GGAGCATCTG CCTCAGGTGC CAGAATGCCC ACTACCTGCT GCTCGGGGAC 
CACTGTGTTC CTGACTGCCC TTCAGGATAC TATGCAGAGA GAGGAGCTTG TAAAAAATGC 
CACTCCTCCT GCAGAACCTG CCAGGGCAGA GGACCTTTCT CCTGCTCCTC ATGTGACACC 
AACCTCGTGC TGTCCCACAC TGGCACCTGC AGCACCACCT GCTTCCCTGG GCACTATCTT 
GATGACAATC ATGTTTGCCA GCCATGCAAC ACACACTGTG GAAGCTGTGA TTCACAGGCC 
AGCTGTACCT CCTGCCGAGA TCCAAACAAG GTTCTGCTCT TTGGGGAATG TCAATACGAG 
AGCTGCGCCC CACAGTACTA TCTTGACTTC TCCACCAACA CGTGCAAAGA GTGTGATTGG 
AGCTGCAGTG CATGCAGTGG GCCCCTGAAA ACAGACTGCC TGCAGTGCAT GGATGGCTAT 
GTTCTCCAGG ATGGGGCCTG CGTGGAGCAG TGCTTGTCAT CATTTTACCA GGACTCGGGC 
CTCTGCAAGA ACTGTGACAG CTACTGTCTC CAGTGCCAAG GTCCCCATGA GTGTACCCGC 
TGCAAAGGGC CATTTCTCCT CTTGGAAGCC CAGTGTGTCC AGGAATGTGG GAAGGGGTAC 
TTTGCAGATC ATGCAAAGCA CAAATGCACA GCCTGCCCTC AGGGGTGCTT GCAGTGCAGC 
CACAGGGACC GTTGTCACCT CTGTGACCAT GGGTTCTTTC TGAAGAGTGG CCTCTGTGTT 
TACAACTGTG TTCCTGGCTT TTCTGTCCAC ACCTCTAATG AAACATGTTC TGGCAAAATA 
CACACCCCTA GTCTTCATGT GAATGGTTCC CTGATCCTCC CAATTGGTTC AATAAAGCCA 
CTGGATTTTT CCCTCCTGAA TGTCCAAGAC CAGGAGGGTA GGGTCGAAGA TCTCCTATTT 
CATGTTGTGA GCACTCCCAC CAATGGTCAG CTAGTGCTCT CAAGAAATGG AAAAGAGGTT 
CAGCTGGACA AGGCTGGCCG TTTTAGCTGG AAAGATGTGA ACGAGAAGAA AGTGCGTTTT 
GTGCACAGCA AAGAAAAACT CAGGAAAGGT TACCTTTTCC TGAAAATTAG TGACCAGCAG 
TTCTTCTCTG AGCCACAGCT GATCAACATA CAAGCATTTT CAACACAGGC CCCCTATGTG 
CTGAGAAATG AAGTTCTCCA CATTAGCAGA GGAGAGAGGG CAACCATCAC CACCCAGATG 
CTTGACATCC GAGATGATGA CAACCCACAG GATGTGGTCA TTGAAATAAT CGATCCTCCA 
CTTCATGGCC AATTGCTTCA GACACTTCAG TCCCCGGCAA CCCCTATCTA TCAATTCCAG 
CTGGATGAAC TCTCTAGAGG CCTTCTCCAC TATGCTCATG ATGGTTCAGA CAGCACATCC 
GATGTTGCAG TCTTGCAGGC CAATGATGGA CACTCCTTCC ATAATATACT GTTCCAAGTG 
AAGACCGTGC CTCAGAATGA CAGGGGTCTT CAGCTTGTGG CTAATTCGAT GGTGTGGGTT 
CCAGAAGGGG GGATGCTGCA GATCACCAAC AGAATCTTAC AGGCCGAGGC TCCTGGTGCC 
AGTGCTGAAG AAATCATCTA CAAGATTACA CAAGACTACC CCCAGTTTGG GGAGGTGGTC 
CTTCTAGTGA ATATGCCTGC AGACAGCCCT GCAGATGAAG GGCAGCACCT GCCTGATGGG 
AGGACAGCTA CCCCCACCAG CACCTTCACC CAGCAGGACA TCAATGAAGG CATCGTATGG 
TACAGGCACT CAGGAGCCCC AGCCCAGAGC GACTCCTTCC GCTTCGAGGT GTCCAGTGCC 
TCCAATGCCC AGACCCGCCT GGAGAGCCAC ATGTTCAACA TCGCGATCTT ACCACAGACA 
CCTGAAGCAC CTAAAGTGTC TCTGGAAGCA TCTCTCCATA TGACTGCTAG AGAAGATGGC 
CTGACTGTTA TTCAGCCTCA TTCCCTCTCC TTCATAAACT CTGAGAAGCC AAGTGGAAAG 
ATTGTCTACA ACATCACTCT ACCTCTGCAT CCAAATCAAG GTATCATCGA GCACCGGGAC 
CACCCTCACT CTCCTATCCG GTATTTCACG CAAGAGGATA TTAACCAGGG CAAAGTCATG 
TACCGCCCTC CCCCGGCAGC ACCCCACCTC CAGGAGCTCA TGGCCTTCTC GTTCGCTGGT 
CTCCCAGAAT CAGTGAAATT CCACTTCACA GTTTCAGATG GAGAACACAC AAGTCCGGAG 
ATGGTCCTCA CCATTCACTT ACTTCCCAGT GATCAGCAAC TGCCAGTGTT CCAGGTCACA 
GCTCCACGGC TGGCGGTCAG CCCAGGAGGC AGCACTTCTG TAGGACTTCA GGTGGTAGTA 
AGAGATGCTG AGACAGCGCC CAAAGAACTC TTCTTTGAGC TTCGGAGACC TCCACAGCAT 
GGTGTGCTTC TTAAGCATAC AGCTGAGTTC CGAAGGCCGA TGGCCACAGG TGACACTTTC 
ACCTATGAGG ATGTTGAGAA AAATGCTCTA CAGTATATAC ATGATGGTTC CTCTACCCGG 
GAAGACAGCA TGGAGATCTC AGTCACAGAT GGCCTCACAG TGACAATGCT GGAGGTGAGA 
GTAGAGGTGT CCCTGTCAGA AGACCGAGGG CCTCGACTGG CTGCTGGCTC CTCTCTGAGC 
ATTACTGTTG CCAGTAAAAG CACAGCCATA ATCACTAGGT CACACCTTGC TTACGTGGAT 
GATTCTTCCC CCGACCCAGA GATCTGGATT CAGTTAAATT ATCTGCCCTC ATATGGTACT 
CTCTTAAGAA TCTCAGGATC TGAGGTGGAA GAGCTCTCAG AAGTTTCCAA TTTCACAATG 
GAAGACATCA ATAACAAGAA AATCAGATAC TCAGCTGTGT TTGAAACTGA TGGTCATCTG 
GTTACTGATA GCTTCTATTT CTCTGTCTCT GACATGGACC ACAACCATCT GGATAATCAG 
ATATTTACCA TCATGATCAC TCCTGCTGAA AATCCACCTC CAGTCATTGC TTTTGCTGAC 
CTTATCACGG TTGATGAGGG AGGGAGAGCA CCACTCTCAT TTCACCATTT TTTTGCTACT 
GATGATGATG ACAACCTCCA GAGAGATGCC ATCATTAAAC TAAGTGCTCT GCCCAAATAT 
GGCTGCATTG AGAACACAGG AACAGGTGAT CGTTTTGGCC CTGAAACTGC CAGTGACCTA 
GAGGCATCAT TTCCTATTCA AGACGTCCTG GAAAACTACA TTTACTACTT TCAGAGTGTT 
CATGAAAGCA TTGAGCCAAC CCATGATATT TTTAGTTTTT ATGTGAGTGA TGGAACCAGT 
CGTTCAGAAA TTCACAGCAT CAATATCACC ATTGAGGTAA AGACTTTGGA AGTTGGAAAG 
GTTGAGCCCT TGACCACAAT ATTTCACACC ATTAGAGAGC TTTCTTTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159605.1
CDS	441..6371
Translation	MGVLKVWLGLALALAEFAVLPHHSEGACVYQDSLLADATIWKPDSCQSCRCHGDIVICKPAVCRNPQCAFEKGEVLQIAANQCCPECVLRTPGSCHHEKKIHEHGTEWASSPCSVCSCNHGEVRCTPQPCPPLSCGHQELAFIPEGSCCPVCVGLGKPCSYEGHVFQDGEDWRLSRCAKCLCRNGVAQCFTAQCQPLFCNQDETVVRVPGKCCPQCSARSCSAAGQVYEHGEQWSENACTTCICDRGEVRCHKQACLPLRCGKGQSRARRHGQCCEECVSPAGSCSYDGVVRYQDEMWKGSACEFCMCDHGQVTCQTGECAKVECARDEELIHLDGKCCPECISRNGYCVYEETGEFMSSNASEVKRIPEGEKWEDGPCKVCECRGAQVTCYEPSCPPCPVGTLALEVKGQCCPDCTSVHCHPDCLTCSQSPDHCDLCQDPTKLLQNGWCVHSCGLGFYQAGSLCLACQPQCSTCTSGLECSSCQPPLLMRHGQCVPTCGDGFYQDRHSCAVCHESCAGCWGPTEKHCLACRDPLHVLRDGGCESSCGKGFYNRQGTCSACDQSCDSCGPSSPRCLTCTEKTVLHDGKCMSECPGGYYADATGRCKVCHNSCASCSGPTPSHCTACSPPKALRQGHCLPRCGEGFYSDHGVCKACHSSCLACMGPAPSHCTGCKKPEEGLQVEQLSDVGIPSGECLAQCRAHFYLESTGICEACHQSCFRCAGKSPHNCTDCGPSHVLLDGQCLSQCPDGYFHQEGSCTECHPTCRQCHGPLESDCISCYPHISLTNGNCRTSCREEQFLNLVGYCADCHHLCQHCAADLHNTGSICLRCQNAHYLLLGDHCVPDCPSGYYAERGACKKCHSSCRTCQGRGPFSCSSCDTNLVLSHTGTCSTTCFPGHYLDDNHVCQPCNTHCGSCDSQASCTSCRDPNKVLLFGECQYESCAPQYYLDFSTNTCKECDWSCSACSGPLKTDCLQCMDGYVLQDGACVEQCLSSFYQDSGLCKNCDSYCLQCQGPHECTRCKGPFLLLEAQCVQECGKGYFADHAKHKCTACPQGCLQCSHRDRCHLCDHGFFLKSGLCVYNCVPGFSVHTSNETCSGKIHTPSLHVNGSLILPIGSIKPLDFSLLNVQDQEGRVEDLLFHVVSTPTNGQLVLSRNGKEVQLDKAGRFSWKDVNEKKVRFVHSKEKLRKGYLFLKISDQQFFSEPQLINIQAFSTQAPYVLRNEVLHISRGERATITTQMLDIRDDDNPQDVVIEIIDPPLHGQLLQTLQSPATPIYQFQLDELSRGLLHYAHDGSDSTSDVAVLQANDGHSFHNILFQVKTVPQNDRGLQLVANSMVWVPEGGMLQITNRILQAEAPGASAEEIIYKITQDYPQFGEVVLLVNMPADSPADEGQHLPDGRTATPTSTFTQQDINEGIVWYRHSGAPAQSDSFRFEVSSASNAQTRLESHMFNIAILPQTPEAPKVSLEASLHMTAREDGLTVIQPHSLSFINSEKPSGKIVYNITLPLHPNQGIIEHRDHPHSPIRYFTQEDINQGKVMYRPPPAAPHLQELMAFSFAGLPESVKFHFTVSDGEHTSPEMVLTIHLLPSDQQLPVFQVTAPRLAVSPGGSTSVGLQVVVRDAETAPKELFFELRRPPQHGVLLKHTAEFRRPMATGDTFTYEDVEKNALQYIHDGSSTREDSMEISVTDGLTVTMLEVRVEVSLSEDRGPRLAAGSSLSITVASKSTAIITRSHLAYVDDSSPDPEIWIQLNYLPSYGTLLRISGSEVEELSEVSNFTMEDINNKKIRYSAVFETDGHLVTDSFYFSVSDMDHNHLDNQIFTIMITPAENPPPVIAFADLITVDEGGRAPLSFHHFFATDDDDNLQRDAIIKLSALPKYGCIENTGTGDRFGPETASDLEASFPIQDVLENYIYYFQSVHESIEPTHDIFSFYVSDGTSRSEIHSINITIEVKTLEVGKVEPLTTIFHTIRELSL

Target ORF information:

RefSeq Version	NM_001166133.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166133.1

ORF Insert Sequence:

ATGGGTGTCC TCAAAGTGTG GCTCGGGCTG GCCCTAGCGT TGGCGGAATT TGCAGTATTG 
CCTCATCATT CCGAAGGTGC TTGTGTCTAT CAGGATTCCT TGTTGGCGGA TGCCACAATT 
TGGAAGCCCG ATTCATGCCA GAGCTGCCGT TGCCATGGTG ATATTGTTAT CTGCAAACCT 
GCTGTTTGCA GAAACCCTCA ATGTGCCTTT GAGAAGGGAG AAGTGCTTCA AATAGCTGCC 
AACCAATGCT GTCCTGAGTG TGTTTTGAGG ACTCCAGGAT CTTGCCATCA TGAAAAGAAA 
ATCCATGAGC ATGGGACAGA ATGGGCCTCT TCTCCATGTA GTGTGTGCTC TTGCAATCAT 
GGGGAAGTCC GATGTACCCC CCAACCATGC CCACCGCTGT CATGTGGACA CCAGGAGCTG 
GCATTCATCC CTGAAGGAAG CTGCTGCCCA GTTTGTGTGG GCCTTGGGAA ACCCTGTTCC 
TATGAAGGCC ATGTGTTTCA GGATGGGGAG GACTGGCGGC TGAGCCGGTG TGCCAAATGT 
CTGTGTAGAA ATGGGGTTGC CCAGTGCTTC ACAGCTCAGT GTCAGCCTCT ATTTTGTAAC 
CAGGATGAGA CTGTAGTCCG AGTCCCTGGA AAATGTTGCC CGCAGTGCTC TGCAAGATCC 
TGCTCTGCAG CTGGCCAAGT ATACGAGCAT GGTGAGCAGT GGAGCGAAAA TGCCTGCACC 
ACGTGTATAT GTGACCGGGG TGAGGTCAGG TGTCACAAGC AGGCCTGCCT GCCCCTGAGA 
TGCGGAAAGG GTCAGAGCAG GGCTCGGCGT CATGGGCAAT GCTGTGAGGA ATGTGTGTCT 
CCTGCCGGGA GCTGCTCCTA TGATGGAGTT GTGCGGTACC AGGACGAAAT GTGGAAGGGC 
TCGGCCTGTG AGTTCTGCAT GTGTGATCAT GGCCAAGTGA CCTGCCAGAC TGGAGAGTGT 
GCCAAAGTGG AGTGTGCCCG GGATGAAGAA TTAATTCACT TAGATGGAAA GTGTTGTCCT 
GAATGCATTT CAAGGAATGG TTATTGTGTT TATGAAGAAA CTGGAGAATT TATGTCATCA 
AATGCTAGTG AAGTTAAACG TATTCCAGAG GGAGAGAAGT GGGAAGATGG CCCTTGCAAG 
GTGTGTGAGT GCCGAGGGGC TCAGGTAACT TGCTACGAGC CCTCTTGCCC ACCATGTCCA 
GTGGGCACAC TGGCCTTAGA GGTGAAGGGA CAGTGCTGTC CAGACTGCAC ATCAGTTCAT 
TGCCATCCAG ATTGTTTGAC ATGCTCTCAG TCTCCAGACC ACTGTGACCT CTGCCAAGAT 
CCTACCAAGT TACTGCAGAA TGGATGGTGT GTGCACAGCT GTGGACTGGG TTTTTACCAA 
GCTGGCAGTC TCTGTTTAGC CTGCCAGCCC CAGTGCTCCA CGTGTACCAG TGGGCTGGAG 
TGCTCATCCT GCCAGCCTCC CCTGCTGATG CGGCACGGGC AGTGTGTGCC TACCTGTGGG 
GACGGCTTCT ACCAAGATCG CCATTCCTGT GCAGTCTGCC ATGAGTCCTG TGCAGGTTGC 
TGGGGCCCAA CGGAGAAGCA CTGCTTGGCC TGCAGAGATC CCCTCCACGT GCTGAGAGAT 
GGCGGCTGTG AGAGCAGCTG TGGAAAAGGC TTCTACAACA GGCAGGGCAC CTGTAGCGCT 
TGTGACCAAT CCTGTGACAG TTGTGGCCCC AGTAGCCCCA GGTGTCTTAC CTGTACTGAG 
AAGACAGTGC TGCATGATGG GAAATGCATG TCTGAATGCC CTGGCGGGTA CTATGCTGAT 
GCCACTGGCA GGTGCAAAGT TTGTCATAAC TCATGTGCCA GCTGCTCTGG GCCCACACCC 
TCTCACTGTA CAGCCTGCAG CCCCCCCAAG GCTCTGCGTC AAGGCCACTG TCTGCCCCGC 
TGTGGAGAGG GTTTCTACTC TGACCATGGA GTCTGCAAAG CCTGTCACTC CTCCTGCCTG 
GCTTGTATGG GTCCCGCACC CTCTCACTGT ACTGGGTGTA AGAAGCCAGA GGAAGGACTG 
CAAGTGGAGC AGCTGTCTGA CGTGGGCATC CCCTCTGGCG AGTGTCTAGC CCAGTGTAGA 
GCCCATTTTT ACTTGGAGAG CACTGGCATA TGTGAAGCTT GCCACCAGTC CTGTTTCAGA 
TGTGCAGGGA AAAGCCCACA TAACTGCACA GACTGTGGGC CTTCCCATGT GCTGTTGGAT 
GGGCAGTGCC TCTCCCAGTG CCCAGATGGC TACTTTCACC AGGAAGGTAG TTGCACAGAG 
TGTCACCCAA CCTGCAGGCA GTGTCATGGG CCGTTGGAGT CTGACTGCAT CTCCTGTTAC 
CCTCACATCT CTCTTACCAA TGGTAACTGC AGGACCAGCT GCAGGGAAGA GCAGTTCCTC 
AACCTCGTGG GATACTGTGC TGACTGCCAT CACCTGTGCC AGCACTGTGC AGCTGATCTC 
CACAACACTG GGAGCATCTG CCTCAGGTGC CAGAATGCCC ACTACCTGCT GCTCGGGGAC 
CACTGTGTTC CTGACTGCCC TTCAGGATAC TATGCAGAGA GAGGAGCTTG TAAAAAATGC 
CACTCCTCCT GCAGAACCTG CCAGGGCAGA GGACCTTTCT CCTGCTCCTC ATGTGACACC 
AACCTCGTGC TGTCCCACAC TGGCACCTGC AGCACCACCT GCTTCCCTGG GCACTATCTT 
GATGACAATC ATGTTTGCCA GCCATGCAAC ACACACTGTG GAAGCTGTGA TTCACAGGCC 
AGCTGTACCT CCTGCCGAGA TCCAAACAAG GTTCTGCTCT TTGGGGAATG TCAATACGAG 
AGCTGCGCCC CACAGTACTA TCTTGACTTC TCCACCAACA CGTGCAAAGA GTGTGATTGG 
AGCTGCAGTG CATGCAGTGG GCCCCTGAAA ACAGACTGCC TGCAGTGCAT GGATGGCTAT 
GTTCTCCAGG ATGGGGCCTG CGTGGAGCAG TGCTTGTCAT CATTTTACCA GGACTCGGGC 
CTCTGCAAGA ACTGTGACAG CTACTGTCTC CAGTGCCAAG GTCCCCATGA GTGTACCCGC 
TGCAAAGGGC CATTTCTCCT CTTGGAAGCC CAGTGTGTCC AGGAATGTGG GAAGGGGTAC 
TTTGCAGATC ATGCAAAGCA CAAATGCACA GCCTGCCCTC AGGGGTGCTT GCAGTGCAGC 
CACAGGGACC GTTGTCACCT CTGTGACCAT GGGTTCTTTC TGAAGAGTGG CCTCTGTGTT 
TACAACTGTG TTCCTGGCTT TTCTGTCCAC ACCTCTAATG AAACATGTTC TGGCAAAATA 
CACACCCCTA GTCTTCATGT GAATGGTTCC CTGATCCTCC CAATTGGTTC AATAAAGCCA 
CTGGATTTTT CCCTCCTGAA TGTCCAAGAC CAGGAGGGTA GGGTCGAAGA TCTCCTATTT 
CATGTTGTGA GCACTCCCAC CAATGGTCAG CTAGTGCTCT CAAGAAATGG AAAAGAGGTT 
CAGCTGGACA AGGCTGGCCG TTTTAGCTGG AAAGATGTGA ACGAGAAGAA AGTGCGTTTT 
GTGCACAGCA AAGAAAAACT CAGGAAAGGT TACCTTTTCC TGAAAATTAG TGACCAGCAG 
TTCTTCTCTG AGCCACAGCT GATCAACATA CAAGCATTTT CAACACAGGC CCCCTATGTG 
CTGAGAAATG AAGTTCTCCA CATTAGCAGA GGAGAGAGGG CAACCATCAC CACCCAGATG 
CTTGACATCC GAGATGATGA CAACCCACAG GATGTGGTCA TTGAAATAAT CGATCCTCCA 
CTTCATGGCC AATTGCTTCA GACACTTCAG TCCCCGGCAA CCCCTATCTA TCAATTCCAG 
CTGGATGAAC TCTCTAGAGG CCTTCTCCAC TATGCTCATG ATGGTTCAGA CAGCACATCC 
GATGTTGCAG TCTTGCAGGC CAATGATGGA CACTCCTTCC ATAATATACT GTTCCAAGTG 
AAGACCGTGC CTCAGAATGA CAGGGGTCTT CAGCTTGTGG CTAATTCGAT GGTGTGGGTT 
CCAGAAGGGG GGATGCTGCA GATCACCAAC AGAATCTTAC AGGCCGAGGC TCCTGGTGCC 
AGTGCTGAAG AAATCATCTA CAAGATTACA CAAGACTACC CCCAGTTTGG GGAGGTGGTC 
CTTCTAGTGA ATATGCCTGC AGACAGCCCT GCAGATGAAG GGCAGCACCT GCCTGATGGG 
AGGACAGCTA CCCCCACCAG CACCTTCACC CAGCAGGACA TCAATGAAGG CATCGTATGG 
TACAGGCACT CAGGAGCCCC AGCCCAGAGC GACTCCTTCC GCTTCGAGGT GTCCAGTGCC 
TCCAATGCCC AGACCCGCCT GGAGAGCCAC ATGTTCAACA TCGCGATCTT ACCACAGACA 
CCTGAAGCAC CTAAAGTGTC TCTGGAAGCA TCTCTCCATA TGACTGCTAG AGAAGATGGC 
CTGACTGTTA TTCAGCCTCA TTCCCTCTCC TTCATAAACT CTGAGAAGCC AAGTGGAAAG 
ATTGTCTACA ACATCACTCT ACCTCTGCAT CCAAATCAAG GTATCATCGA GCACCGGGAC 
CACCCTCACT CTCCTATCCG GTATTTCACG CAAGAGGATA TTAACCAGGG CAAAGTCATG 
TACCGCCCTC CCCCGGCAGC ACCCCACCTC CAGGAGCTCA TGGCCTTCTC GTTCGCTGGT 
CTCCCAGAAT CAGTGAAATT CCACTTCACA GTTTCAGATG GAGAACACAC AAGTCCGGAG 
ATGGTCCTCA CCATTCACTT ACTTCCCAGT GATCAGCAAC TGCCAGTGTT CCAGGTCACA 
GCTCCACGGC TGGCGGTCAG CCCAGGAGGC AGCACTTCTG TAGGACTTCA GGTGGTAGTA 
AGAGATGCTG AGACAGCGCC CAAAGAACTC TTCTTTGAGC TTCGGAGACC TCCACAGCAT 
GGTGTGCTTC TTAAGCATAC AGCTGAGTTC CGAAGGCCGA TGGCCACAGG TGACACTTTC 
ACCTATGAGG ATGTTGAGAA AAATGCTCTA CAGTATATAC ATGATGGTTC CTCTACCCGG 
GAAGACAGCA TGGAGATCTC AGTCACAGAT GGCCTCACAG TGACAATGCT GGAGGTGAGA 
GTAGAGGTGT CCCTGTCAGA AGACCGAGGG CCTCGACTGG CTGCTGGCTC CTCTCTGAGC 
ATTACTGTTG CCAGTAAAAG CACAGCCATA ATCACTAGGT CACACCTTGC TTACGTGGAT 
GATTCTTCCC CCGACCCAGA GATCTGGATT CAGTTAAATT ATCTGCCCTC ATATGGTACT 
CTCTTAAGAA TCTCAGGATC TGAGGTGGAA GAGCTCTCAG AAGTTTCCAA TTTCACAATG 
GAAGACATCA ATAACAAGAA AATCAGATAC TCAGCTGTGT TTGAAACTGA TGGTCATCTG 
GTTACTGATA GCTTCTATTT CTCTGTCTCT GACATGGACC ACAACCATCT GGATAATCAG 
ATATTTACCA TCATGATCAC TCCTGCTGAA AATCCACCTC CAGTCATTGC TTTTGCTGAC 
CTTATCACGG TTGATGAGGG AGGGAGAGCA CCACTCTCAT TTCACCATTT TTTTGCTACT 
GATGATGATG ACAACCTCCA GAGAGATGCC ATCATTAAAC TAAGTGCTCT GCCCAAATAT 
GGCTGCATTG AGAACACAGG AACAGGTGAT CGTTTTGGCC CTGAAACTGC CAGTGACCTA 
GAGGCATCAT TTCCTATTCA AGACGTCCTG GAAAACTACA TTTACTACTT TCAGAGTGTT 
CATGAAAGCA TTGAGCCAAC CCATGATATT TTTAGTTTTT ATGTGAGTGA TGGAACCAGT 
CGTTCAGAAA TTCACAGCAT CAATATCACC ATTGAGGTAA AGACTTTGGA AGTTGGAAAG 
GTTGAGCCCT TGACCACAAT ATTTCACACC ATTAGAGAGC TTTCTTTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FRAS1 ( NM_025074.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_079350.5 Homo sapiens Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant 1, mRNA.

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CloneID	OHu28517
Clone ID Related Accession (Same CDS sequence)	NM_025074.7 , NM_025074.6
Accession Version	NM_025074.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	extracellular matrix protein FRAS1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC093652.5, DA725113.1, DA881824.1, BC131820.1, AC093886.2, AB040933.2, AC104808.4, BX647949.1 and BX647420.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_025074.6. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology MANE Ensembl match :: ENST00000512123.4/ ENSP00000422834.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGTGTCC TCAAAGTGTG GCTCGGGCTG GCCCTAGCGT TGGCGGAATT TGCAGTATTG 
CCTCATCATT CCGAAGGTGC TTGTGTCTAT CAGGATTCCT TGTTGGCGGA TGCCACAATT 
TGGAAGCCCG ATTCATGCCA GAGCTGCCGT TGCCATGGTG ATATTGTTAT CTGCAAACCT 
GCTGTTTGCA GAAACCCTCA ATGTGCCTTT GAGAAGGGAG AAGTGCTTCA AATAGCTGCC 
AACCAATGCT GTCCTGAGTG TGTTTTGAGG ACTCCAGGAT CTTGCCATCA TGAAAAGAAA 
ATCCATGAGC ATGGGACAGA ATGGGCCTCT TCTCCATGTA GTGTGTGCTC TTGCAATCAT 
GGGGAAGTCC GATGTACCCC CCAACCATGC CCACCGCTGT CATGTGGACA CCAGGAGCTG 
GCATTCATCC CTGAAGGAAG CTGCTGCCCA GTTTGTGTGG GCCTTGGGAA ACCCTGTTCC 
TATGAAGGCC ATGTGTTTCA GGATGGGGAG GACTGGCGGC TGAGCCGGTG TGCCAAATGT 
CTGTGTAGAA ATGGGGTTGC CCAGTGCTTC ACAGCTCAGT GTCAGCCTCT ATTTTGTAAC 
CAGGATGAGA CTGTAGTCCG AGTCCCTGGA AAATGTTGCC CGCAGTGCTC TGCAAGATCC 
TGCTCTGCAG CTGGCCAAGT ATACGAGCAT GGTGAGCAGT GGAGCGAAAA TGCCTGCACC 
ACGTGTATAT GTGACCGGGG TGAGGTCAGG TGTCACAAGC AGGCCTGCCT GCCCCTGAGA 
TGCGGAAAGG GTCAGAGCAG GGCTCGGCGT CATGGGCAAT GCTGTGAGGA ATGTGTGTCT 
CCTGCCGGGA GCTGCTCCTA TGATGGAGTT GTGCGGTACC AGGACGAAAT GTGGAAGGGC 
TCGGCCTGTG AGTTCTGCAT GTGTGATCAT GGCCAAGTGA CCTGCCAGAC TGGAGAGTGT 
GCCAAAGTGG AGTGTGCCCG GGATGAAGAA TTAATTCACT TAGATGGAAA GTGTTGTCCT 
GAATGCATTT CAAGGAATGG TTATTGTGTT TATGAAGAAA CTGGAGAATT TATGTCATCA 
AATGCTAGTG AAGTTAAACG TATTCCAGAG GGAGAGAAGT GGGAAGATGG CCCTTGCAAG 
GTGTGTGAGT GCCGAGGGGC TCAGGTAACT TGCTACGAGC CCTCTTGCCC ACCATGTCCA 
GTGGGCACAC TGGCCTTAGA GGTGAAGGGA CAGTGCTGTC CAGACTGCAC ATCAGTTCAT 
TGCCATCCAG ATTGTTTGAC ATGCTCTCAG TCTCCAGACC ACTGTGACCT CTGCCAAGAT 
CCTACCAAGT TACTGCAGAA TGGATGGTGT GTGCACAGCT GTGGACTGGG TTTTTACCAA 
GCTGGCAGTC TCTGTTTAGC CTGCCAGCCC CAGTGCTCCA CGTGTACCAG TGGGCTGGAG 
TGCTCATCCT GCCAGCCTCC CCTGCTGATG CGGCACGGGC AGTGTGTGCC TACCTGTGGG 
GACGGCTTCT ACCAAGATCG CCATTCCTGT GCAGTCTGCC ATGAGTCCTG TGCAGGTTGC 
TGGGGCCCAA CGGAGAAGCA CTGCTTGGCC TGCAGAGATC CCCTCCACGT GCTGAGAGAT 
GGCGGCTGTG AGAGCAGCTG TGGAAAAGGC TTCTACAACA GGCAGGGCAC CTGTAGCGCT 
TGTGACCAAT CCTGTGACAG TTGTGGCCCC AGTAGCCCCA GGTGTCTTAC CTGTACTGAG 
AAGACAGTGC TGCATGATGG GAAATGCATG TCTGAATGCC CTGGCGGGTA CTATGCTGAT 
GCCACTGGCA GGTGCAAAGT TTGTCATAAC TCATGTGCCA GCTGCTCTGG GCCCACACCC 
TCTCACTGTA CAGCCTGCAG CCCCCCCAAG GCTCTGCGTC AAGGCCACTG TCTGCCCCGC 
TGTGGAGAGG GTTTCTACTC TGACCATGGA GTCTGCAAAG CCTGTCACTC CTCCTGCCTG 
GCTTGTATGG GTCCCGCACC CTCTCACTGT ACTGGGTGTA AGAAGCCAGA GGAAGGACTG 
CAAGTGGAGC AGCTGTCTGA CGTGGGCATC CCCTCTGGCG AGTGTCTAGC CCAGTGTAGA 
GCCCATTTTT ACTTGGAGAG CACTGGCATA TGTGAAGCTT GCCACCAGTC CTGTTTCAGA 
TGTGCAGGGA AAAGCCCACA TAACTGCACA GACTGTGGGC CTTCCCATGT GCTGTTGGAT 
GGGCAGTGCC TCTCCCAGTG CCCAGATGGC TACTTTCACC AGGAAGGTAG TTGCACAGAG 
TGTCACCCAA CCTGCAGGCA GTGTCATGGG CCGTTGGAGT CTGACTGCAT CTCCTGTTAC 
CCTCACATCT CTCTTACCAA TGGTAACTGC AGGACCAGCT GCAGGGAAGA GCAGTTCCTC 
AACCTCGTGG GATACTGTGC TGACTGCCAT CACCTGTGCC AGCACTGTGC AGCTGATCTC 
CACAACACTG GGAGCATCTG CCTCAGGTGC CAGAATGCCC ACTACCTGCT GCTCGGGGAC 
CACTGTGTTC CTGACTGCCC TTCAGGATAC TATGCAGAGA GAGGAGCTTG TAAAAAATGC 
CACTCCTCCT GCAGAACCTG CCAGGGCAGA GGACCTTTCT CCTGCTCCTC ATGTGACACC 
AACCTCGTGC TGTCCCACAC TGGCACCTGC AGCACCACCT GCTTCCCTGG GCACTATCTT 
GATGACAATC ATGTTTGCCA GCCATGCAAC ACACACTGTG GAAGCTGTGA TTCACAGGCC 
AGCTGTACCT CCTGCCGAGA TCCAAACAAG GTTCTGCTCT TTGGGGAATG TCAATACGAG 
AGCTGCGCCC CACAGTACTA TCTTGACTTC TCCACCAACA CGTGCAAAGA GTGTGATTGG 
AGCTGCAGTG CATGCAGTGG GCCCCTGAAA ACAGACTGCC TGCAGTGCAT GGATGGCTAT 
GTTCTCCAGG ATGGGGCCTG CGTGGAGCAG TGCTTGTCAT CATTTTACCA GGACTCGGGC 
CTCTGCAAGA ACTGTGACAG CTACTGTCTC CAGTGCCAAG GTCCCCATGA GTGTACCCGC 
TGCAAAGGGC CATTTCTCCT CTTGGAAGCC CAGTGTGTCC AGGAATGTGG GAAGGGGTAC 
TTTGCAGATC ATGCAAAGCA CAAATGCACA GCCTGCCCTC AGGGGTGCTT GCAGTGCAGC 
CACAGGGACC GTTGTCACCT CTGTGACCAT GGGTTCTTTC TGAAGAGTGG CCTCTGTGTT 
TACAACTGTG TTCCTGGCTT TTCTGTCCAC ACCTCTAATG AAACATGTTC TGGCAAAATA 
CACACCCCTA GTCTTCATGT GAATGGTTCC CTGATCCTCC CAATTGGTTC AATAAAGCCA 
CTGGATTTTT CCCTCCTGAA TGTCCAAGAC CAGGAGGGTA GGGTCGAAGA TCTCCTATTT 
CATGTTGTGA GCACTCCCAC CAATGGTCAG CTAGTGCTCT CAAGAAATGG AAAAGAGGTT 
CAGCTGGACA AGGCTGGCCG TTTTAGCTGG AAAGATGTGA ACGAGAAGAA AGTGCGTTTT 
GTGCACAGCA AAGAAAAACT CAGGAAAGGT TACCTTTTCC TGAAAATTAG TGACCAGCAG 
TTCTTCTCTG AGCCACAGCT GATCAACATA CAAGCATTTT CAACACAGGC CCCCTATGTG 
CTGAGAAATG AAGTTCTCCA CATTAGCAGA GGAGAGAGGG CAACCATCAC CACCCAGATG 
CTTGACATCC GAGATGATGA CAACCCACAG GATGTGGTCA TTGAAATAAT CGATCCTCCA 
CTTCATGGCC AATTGCTTCA GACACTTCAG TCCCCGGCAA CCCCTATCTA TCAATTCCAG 
CTGGATGAAC TCTCTAGAGG CCTTCTCCAC TATGCTCATG ATGGTTCAGA CAGCACATCC 
GATGTTGCAG TCTTGCAGGC CAATGATGGA CACTCCTTCC ATAATATACT GTTCCAAGTG 
AAGACCGTGC CTCAGAATGA CAGGGGTCTT CAGCTTGTGG CTAATTCGAT GGTGTGGGTT 
CCAGAAGGGG GGATGCTGCA GATCACCAAC AGAATCTTAC AGGCCGAGGC TCCTGGTGCC 
AGTGCTGAAG AAATCATCTA CAAGATTACA CAAGACTACC CCCAGTTTGG GGAGGTGGTC 
CTTCTAGTGA ATATGCCTGC AGACAGCCCT GCAGATGAAG GGCAGCACCT GCCTGATGGG 
AGGACAGCTA CCCCCACCAG CACCTTCACC CAGCAGGACA TCAATGAAGG CATCGTATGG 
TACAGGCACT CAGGAGCCCC AGCCCAGAGC GACTCCTTCC GCTTCGAGGT GTCCAGTGCC 
TCCAATGCCC AGACCCGCCT GGAGAGCCAC ATGTTCAACA TCGCGATCTT ACCACAGACA 
CCTGAAGCAC CTAAAGTGTC TCTGGAAGCA TCTCTCCATA TGACTGCTAG AGAAGATGGC 
CTGACTGTTA TTCAGCCTCA TTCCCTCTCC TTCATAAACT CTGAGAAGCC AAGTGGAAAG 
ATTGTCTACA ACATCACTCT ACCTCTGCAT CCAAATCAAG GTATCATCGA GCACCGGGAC 
CACCCTCACT CTCCTATCCG GTATTTCACG CAAGAGGATA TTAACCAGGG CAAAGTCATG 
TACCGCCCTC CCCCGGCAGC ACCCCACCTC CAGGAGCTCA TGGCCTTCTC GTTCGCTGGT 
CTCCCAGAAT CAGTGAAATT CCACTTCACA GTTTCAGATG GAGAACACAC AAGTCCGGAG 
ATGGTCCTCA CCATTCACTT ACTTCCCAGT GATCAGCAAC TGCCAGTGTT CCAGGTCACA 
GCTCCACGGC TGGCGGTCAG CCCAGGAGGC AGCACTTCTG TAGGACTTCA GGTGGTAGTA 
AGAGATGCTG AGACAGCGCC CAAAGAACTC TTCTTTGAGC TTCGGAGACC TCCACAGCAT 
GGTGTGCTTC TTAAGCATAC AGCTGAGTTC CGAAGGCCGA TGGCCACAGG TGACACTTTC 
ACCTATGAGG ATGTTGAGAA AAATGCTCTA CAGTATATAC ATGATGGTTC CTCTACCCGG 
GAAGACAGCA TGGAGATCTC AGTCACAGAT GGCCTCACAG TGACAATGCT GGAGGTGAGA 
GTAGAGGTGT CCCTGTCAGA AGACCGAGGG CCTCGACTGG CTGCTGGCTC CTCTCTGAGC 
ATTACTGTTG CCAGTAAAAG CACAGCCATA ATCACTAGGT CACACCTTGC TTACGTGGAT 
GATTCTTCCC CCGACCCAGA GATCTGGATT CAGTTAAATT ATCTGCCCTC ATATGGTACT 
CTCTTAAGAA TCTCAGGATC TGAGGTGGAA GAGCTCTCAG AAGTTTCCAA TTTCACAATG 
GAAGACATCA ATAACAAGAA AATCAGATAC TCAGCTGTGT TTGAAACTGA TGGTCATCTG 
GTTACTGATA GCTTCTATTT CTCTGTCTCT GACATGGACC ACAACCATCT GGATAATCAG 
ATATTTACCA TCATGATCAC TCCTGCTGAA AATCCACCTC CAGTCATTGC TTTTGCTGAC 
CTTATCACGG TTGATGAGGG AGGGAGAGCA CCACTCTCAT TTCACCATTT TTTTGCTACT 
GATGATGATG ACAACCTCCA GAGAGATGCC ATCATTAAAC TAAGTGCTCT GCCCAAATAT 
GGCTGCATTG AGAACACAGG AACAGGTGAT CGTTTTGGCC CTGAAACTGC CAGTGACCTA 
GAGGCATCAT TTCCTATTCA AGACGTCCTG GAAAACTACA TTTACTACTT TCAGAGTGTT 
CATGAAAGCA TTGAGCCAAC CCATGATATT TTTAGTTTTT ATGTGAGTGA TGGAACCAGT 
CGTTCAGAAA TTCACAGCAT CAATATCACC ATTGAGAGGA AGAACGATGA GCCTCCCAGG 
ATGACCTTGC AGCCCCTCAG AGTGCAGCTG AGCTCGGGAG TGGTGATAAG CAATTCTTCT 
TTGAGCCTGC AAGACCTGGA CACCCCAGAT AATGAGCTCA TTTTTGTATT GACAAAAAAG 
CCTGACCACG GTCATGTACT CTGGAGGCAA ACTGCTTCTG AGCCTCTGGA GAATGGGAGA 
GTTTTAGTCC AGGGCTCAAC CTTCACCTAC CAGGATATCC TAGCTGGGCT GGTTGGGTAT 
GTGCCTAGTG TCCCTGGCAT GGTCGTGGAT GAGTTCCAGT TCTCCCTCAC TGATGGCCTC 
CACGTGGACA CAGGGAGGAT GAAGATCTAC ACAGAACTGC CTGCAAGTGA CACACCTCAC 
TTGGCTATAA ACCAAGGCCT ACAGCTCTCA GCAGGGTCTG TAGCACGCAT CACAGAACAG 
CACTTGAAAG TGACAGATAT TGACTCAGAT GACCATCAGG TTATGTACAT CATGAAGGAA 
GATCCTGGTG CAGGGCGCCT GCAGATGATG AAGCATGGCA ACCTGGAGCA AATTTCTATT 
AAAGGCCCCA TCCGAAGTTT CACCCAGGCA GACATTAGCC AAGGCCACGT AGAATATAGT 
CATGGAACAG GAGAACCTGG AGGGAGCTTT GCTTTTAAAT TTGATGTGGT TGATGGAGAA 
GGCAACAGAT TGATTGACAA GTCATTTTCC ATCAGCATTC TAGAAGACAA ATCCCCACCA 
GTCATCACCA CCAATAAAGG ACTGGTCTTG GATGAAAACT CAGTGAAGAA AATCACCACC 
CTGCAGCTGT CTGCCACTGA CCAGGACAGT GGGCCTACAG AATTGATCTA CAGAATCACC 
AGACAGCCCC AGCTGGGCCA CTTGGAACAT GCAGCATCAC CAGGTATCCA GATTAGTTCC 
TTTACTCAAG CTGATCTGAC TTCACGAAAT GTTCAGTATG TCCATTCTAG TGAGGCTGAG 
AAACATTCAG ATGCCTTCAG CTTTACACTG TCTGATGGAG TCAGTGAGGT GACTCAGACT 
TTCCATATCA CTCTTCACCC TGTCGATGAT TCGCTGCCCG TCGTACAGAA CTTAGGAATG 
CGGGTGCAGG AGGGCATGAG GAAGACCATC ACAGAGTTTG AGCTTAAGGC GGTGGATGCT 
GACACAGAGG CCGAGTCTGT CACATTCACC ATCGTGCAGC CTCCACGCCA TGGCACCATC 
GAGCGAACCA GCAATGGGCA GCATTTCCAC CTCACCTCCA CCTTCACCAT GAAAGATATC 
TACCAGAACC GGGTCAGCTA CAGCCATGAC GGCAGTAACT CCCTCAAGGA CCGGTTCACC 
TTCACTGTTT CTGATGGGAC AAACCCCTTC TTTATCATTG AGGAAGGGGG AAAAGAGATT 
ATGACAGCAG CACCTCAGCC GTTCCGAGTA GACATCCTCC CGGTAGATGA TGGCACGCCT 
AGAATTGTCA CCAACCTGGG ACTCCAGTGG CTGGAATACA TGGATGGCAA GGCAACCAAC 
CTGATCACCA AGAAGGAACT GCTGACCATG GACCCAGACA CCGAGGACGC GCAGCTTGTC 
TATGAGATAA CGACGGGCCC TAAGCATGGC TTTGTGGAGA ACAAGCTGCA GCCTGGCAGA 
GCTGCTGCCA CTTTCACCCA GGAGGATGTG AACTTGGGGT TGATTCGTTA TGTGTTGCAC 
AAGGAGAAGA TCCGTGAGAT GATGGATAGT TTTCAGTTTC TGGTGAAAGA CAGTAAACCC 
AATGTGGTCA GCGACAATGT CTTCCATATC CAGTGGTCAC TCATCAGCTT TAAATATACC 
AGCTACAATG TCAGTGAGAA GGCAGGGTCT GTCAGTGTCA CGGTGCAGAG GACTGGGAAC 
CTGAACCAAT ATGCCATCGT CCTGTGTCGC ACCGAGCAAG GCACCGCCAG CTCCAGCTCC 
AGGGTCAGCT CCCAACCTGG GCAACAGGAC TATGTAGAGT ATGCTGGCCA GGTCCAGTTT 
GATGAGCGAG AGGACACCAA GTCCTGCACC ATTGTCATCA ACGATGATGA CGTGTTTGAA 
AATGTTGAGA GTTTCACTGT GGAGCTCAGC ATGCCAGCTT ATGCCCTGTT AGGGGAATTC 
ACCCAGGCGA AGGTCATTAT CAACGATACC GAGGATGAAC CCACATTAGA GTTTGACAAG 
AAGATCTACT GGGTTAACGA GAGCGCTGGT TTTCTGTTTG CACCTATTGA AAGAAAAGGA 
GATGCAAGCA GCATTGTATC TGCAATTTGC TACACAGTCC CTAAGTCAGC TATGGGAAGT 
AGCCTCTATG CTCTAGAATC AGGCTCTGAT TTTAAATCTA GAGGGATGTC TGCCGCGAGT 
CGTGTGATAT TCGGGCCTGG TGTGACCATG TCCACCTGTG ATGTCATGCT TATTGATGAC 
AGCGAGTATG AAGAGGAAGA AGAGTTTGAG ATTGCCTTGG CAGATGCCTC TGACAATGCC 
CGCATTGGAA GGGTGGCGAC AGCCAAGGTG CTCATTAGTG GTCCCAACGA TGCCTCGACT 
GTGTCCCTGG GCAACACGGC TTTCACTGTC AGTGAGGACG CAGGCACAGT AAAGATTCCA 
GTTATCCGCC ATGGTACTGA CCTCTCTACT TTCGCATCTG TCTGGTGTGC AACGCGGCCC 
TCAGACCCAG CTTCTGCCAC ACCAGGAGTT GACTACGTTC CCAGCTCTCG GAAGGTGGAA 
TTTGGGCCTG GTGTCATTGA ACAGTATTGC ACCTTGACTA TCTTGGATGA CACTCAGTAT 
CCGGTAATTG AAGGACTGGA GACATTTGTG GTTTTCCTCA GCTCAGCACA AGGAGCCGAA 
CTGACCAAAC CCTTCCAGGC AGTCATTGCA ATTAATGACA CATTCCAAGA TGTGCCCAGC 
ATGCAGTTTG CCAAGGATTT GCTCCTAGTG AAGGAGAAGG AGGGTGTCCT GCATGTCCCT 
ATCACTCGGA GCGGAGACCT GAGCTATGAG TCATCAGTGA GGTGCTATAC TCAGAGCCAT 
TCCGCTCAGG TCATGGAGGA CTTTGAGGAG AGACAAAATG CAGACTCTTC ACGGATTACA 
TTTCTCAAAG GGGACAAAGT GAAGAACTGT ACGGTCTATA TCCACGATGA CTCCATGTTT 
GAGCCAGAGG AACAGTTCAG GGTCTACCTC GGCCTTCCTC TTGGAAACCA CTGGAGTGGA 
GCTAGAATTG GAAAGAATAA CATGGCCACC ATCACCATAT CCAATGATGA AGATGCCCCC 
ACCATTGAGT TTGAAGAAGC TGCATACCAA GTCCGGGAAC CCGCAGGCCC AGATGCCATT 
GCGATTCTGA ACATCAAGGT GATCCGCAGA GGGGATCAGA ACAGGACCTC CAAGGTTCGC 
TGCAGCACGC GGGATGGCTC TGCCCAGTCT GGTGTGGATT ATTACCCAAA GAGCCGAGTC 
TTGAAGTTCA GTCCCGGTGT GGATCATATC TTTTTTAAAG TTGAGATCCT GTCCAATGAA 
GACCGGGAAT GGCATGAATC TTTCTCACTA GTCCTTGGCC CAGATGACCC AGTGGAAGCA 
GTTCTTGGGG ATGTGACTAC TGCCACGGTG ACAATTCTAG ACCAGGAGGC AGCAGGGAGC 
CTCATATTGC CAGCACCACC CATTGTGGTC ACACTTGCTG ACTATGACCA TGTGGAAGAA 
GTTACCAAGG AAGGAGTCAA GAAATCCCCC TCCCCAGGCT ACCCACTGGT CTGTGTCACC 
CCCTGCGACC CTCATTTCCC CAGATACGCT GTCATGAAGG AGCGCTGCAG TGAGGCCGGC 
ATCAACCAGA CATCTGTGCA GTTCAGCTGG GAAGTGGCTG CCCCCACTGA TGGCAATGGG 
GCCCGGTCTC CCTTTGAAAC CATCACTGAC AACACACCAT TCACCAGTGT CAACCACATG 
GTCCTGGACA GCATTTACTT CAGCCGGAGG TTCCATGTGC GTTGTGTGGC CAAGGCTGTG 
GACAAGGTGG GCCATGTGGG GACCCCCTTA AGGAGCAACA TTGTTACCAT TGGAACAGAC 
AGTGCTATCT GCCACACACC AGTGGTGGCT GGGACATCCA GAGGCTTCCA GGCTCAGTCC 
TTCATCGCAA CCTTGAAATA CCTGGATGTC AAACATAAGG AGCATCCGAA CAGAATCCAC 
ATTTCGGTGC AGATCCCACA CCAGGATGGA ATGCTGCCCC TTATCTCCAC CATGCCGTTG 
CACAACTTAC ATTTTCTACT GTCTGAGTCC ATCTACAGAC ACCAGCACGT CTGCTCCAAT 
TTAGTTACCA CCTATGACCT GAGAGGCATC TCAGAGGCAG GGTTCCTGGA TGATGTGGTC 
TATGATAGCA CTGCCCTGGG GCCTGGCTAC GATCGCCCCT TCCAGTTTGA CCCCAGCGTG 
CGAGAGCCGA AGACCATCCA GCTCTACAAA CACCTGAACC TGAAGAGCTG CGTGTGGACC 
TTTGATGCTT ATTATGACAT GACTGAGCTG ATTGACGTCT GTGGGGGCTC TGTAACCGCT 
GACTTCCAGG TGAGGGACTC TGCCCAGTCC TTCTTGACAG TGCACGTGCC TCTATATGTG 
TCCTACATCT ATGTGACAGC CCCCAGGGGC TGGGCCTCCT TGGAGCACCA CACCGAGATG 
GAGTTTTCTT TCTTCTATGA CACTGTTCTC TGGAGAACAG GAATCCAGAC AGACAGCGTG 
CTCTCTGCAA GGCTTCAGAT AATAAGAATC TACATTCGAG AGGATGGCCG TCTTGTCATT 
GAATTCAAGA CCCATGCCAA ATTCAGAGGA CAGTTTGTGA TGGAGCATCA CACTCTCCCA 
GAAGTGAAAT CTTTCGTATT GACTCCAGAC CACCTAGGAG GAATTGAATT TGACTTGCAG 
CTATTATGGA GCGCTCAGAC TTTTGATTCT CCACATCAAC TCTGGAGAGC CACAAGCTCT 
TATAACAGGA AGGACTACTC AGGAGAGTAC ACCATCTACC TGATCCCTTG CACAGTGCAG 
CCCACACAGC CATGGGTTGA CCCAGGAGAG AAGCCTTTGG CCTGCACTGC ACATGCCCCA 
GAAAGATTCC TGATACCCAT TGCATTCCAG CAGACCAACC GCCCTGTGCC AGTTGTGTAT 
TCACTTAACA CTGAATTTCA GCTCTGCAAT AATGAGAAGG TGTTCCTAAT GGATCCCAAT 
ACATCTGATA TGTCACTAGC AGAAATGGAT TACAAAGGAG CCTTTTCAAA AGGTCAAATC 
CTTTATGGCC GAGTACTTTG GAATCCAGAA CAAAATCTTA ATTCTGCTTA CAAACTCCAG 
CTGGAGAAAG TCTATCTTTG TACGGGCAAG GATGGTTATG TGCCTTTCTT TGATCCCACG 
GGGACAATCT ACAATGAAGG GCCCCAGTAT GGATGCATTC AGCCAAACAA ACACCTAAAA 
CACAGATTCC TGCTGTTGGA CCGCAATCAG CCAGAGGTAA CTGATAAGTA CTTCCATGAT 
GTGCCTTTTG AGGCTCACTT TGCCTCTGAG TTGCCTGATT TCCATGTGGT CAGTAACATG 
CCAGGTGTGG ATGGATTTAC TCTAAAAGTA GATGCACTCT ATAAGGTGGA AGCAGGACAC 
CAGTGGTATC TCCAGGTCAT CTACATCATT GGCCCTGACA CCATCTCAGG GCCCCGGGTC 
CAGCGCTCTC TCACAGCTCC ACTCAGACGC AACCGAAGGG ACCTGGTAGA GCCCGATGGC 
CAGCTGATCC TTGATGATTC CCTCATCTAT GACAATGAAG GAGACCAAGT CAAGAATGGC 
ACCAATATGA AGTCCCTGAA TCTGGAGATG CAAGAGTTGG CGGTAGCTGC GTCCCTGTCA 
CAGACTGGGG CGTCCATTGG CAGTGCCCTG GCTGCAATCA TGCTTCTACT TCTGGTGTTT 
TTGGTGGCTT GTTTTATCAA CAGGAAATGC CAGAAACAGA GGAAGAAGAA GCCCGCAGAG 
GACATTTTGG AAGAATATCC TCTGAATACC AAGGTAGAAG TGCCCAAGAG GCACCCGGAC 
CGGGTGGAGA AGAACGTGAA TAGACACTAC TGCACTGTGC GGAACGTCAA CATCCTGAGT 
GAGCCTGAGG CGGCTTACAC GTTCAAAGGT GCTAAAGTCA AAAGACTGAA TCTAGAAGTC 
AGAGTTCACA ACAATTTACA AGATGGAACA GAAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079350.5
CDS	688..12726
Translation	MGVLKVWLGLALALAEFAVLPHHSEGACVYQDSLLADATIWKPDSCQSCRCHGDIVICKPAVCRNPQCAFEKGEVLQIAANQCCPECVLRTPGSCHHEKKIHEHGTEWASSPCSVCSCNHGEVRCTPQPCPPLSCGHQELAFIPEGSCCPVCVGLGKPCSYEGHVFQDGEDWRLSRCAKCLCRNGVAQCFTAQCQPLFCNQDETVVRVPGKCCPQCSARSCSAAGQVYEHGEQWSENACTTCICDRGEVRCHKQACLPLRCGKGQSRARRHGQCCEECVSPAGSCSYDGVVRYQDEMWKGSACEFCMCDHGQVTCQTGECAKVECARDEELIHLDGKCCPECISRNGYCVYEETGEFMSSNASEVKRIPEGEKWEDGPCKVCECRGAQVTCYEPSCPPCPVGTLALEVKGQCCPDCTSVHCHPDCLTCSQSPDHCDLCQDPTKLLQNGWCVHSCGLGFYQAGSLCLACQPQCSTCTSGLECSSCQPPLLMRHGQCVPTCGDGFYQDRHSCAVCHESCAGCWGPTEKHCLACRDPLHVLRDGGCESSCGKGFYNRQGTCSACDQSCDSCGPSSPRCLTCTEKTVLHDGKCMSECPGGYYADATGRCKVCHNSCASCSGPTPSHCTACSPPKALRQGHCLPRCGEGFYSDHGVCKACHSSCLACMGPAPSHCTGCKKPEEGLQVEQLSDVGIPSGECLAQCRAHFYLESTGICEACHQSCFRCAGKSPHNCTDCGPSHVLLDGQCLSQCPDGYFHQEGSCTECHPTCRQCHGPLESDCISCYPHISLTNGNCRTSCREEQFLNLVGYCADCHHLCQHCAADLHNTGSICLRCQNAHYLLLGDHCVPDCPSGYYAERGACKKCHSSCRTCQGRGPFSCSSCDTNLVLSHTGTCSTTCFPGHYLDDNHVCQPCNTHCGSCDSQASCTSCRDPNKVLLFGECQYESCAPQYYLDFSTNTCKECDWSCSACSGPLKTDCLQCMDGYVLQDGACVEQCLSSFYQDSGLCKNCDSYCLQCQGPHECTRCKGPFLLLEAQCVQECGKGYFADHAKHKCTACPQGCLQCSHRDRCHLCDHGFFLKSGLCVYNCVPGFSVHTSNETCSGKIHTPSLHVNGSLILPIGSIKPLDFSLLNVQDQEGRVEDLLFHVVSTPTNGQLVLSRNGKEVQLDKAGRFSWKDVNEKKVRFVHSKEKLRKGYLFLKISDQQFFSEPQLINIQAFSTQAPYVLRNEVLHISRGERATITTQMLDIRDDDNPQDVVIEIIDPPLHGQLLQTLQSPATPIYQFQLDELSRGLLHYAHDGSDSTSDVAVLQANDGHSFHNILFQVKTVPQNDRGLQLVANSMVWVPEGGMLQITNRILQAEAPGASAEEIIYKITQDYPQFGEVVLLVNMPADSPADEGQHLPDGRTATPTSTFTQQDINEGIVWYRHSGAPAQSDSFRFEVSSASNAQTRLESHMFNIAILPQTPEAPKVSLEASLHMTAREDGLTVIQPHSLSFINSEKPSGKIVYNITLPLHPNQGIIEHRDHPHSPIRYFTQEDINQGKVMYRPPPAAPHLQELMAFSFAGLPESVKFHFTVSDGEHTSPEMVLTIHLLPSDQQLPVFQVTAPRLAVSPGGSTSVGLQVVVRDAETAPKELFFELRRPPQHGVLLKHTAEFRRPMATGDTFTYEDVEKNALQYIHDGSSTREDSMEISVTDGLTVTMLEVRVEVSLSEDRGPRLAAGSSLSITVASKSTAIITRSHLAYVDDSSPDPEIWIQLNYLPSYGTLLRISGSEVEELSEVSNFTMEDINNKKIRYSAVFETDGHLVTDSFYFSVSDMDHNHLDNQIFTIMITPAENPPPVIAFADLITVDEGGRAPLSFHHFFATDDDDNLQRDAIIKLSALPKYGCIENTGTGDRFGPETASDLEASFPIQDVLENYIYYFQSVHESIEPTHDIFSFYVSDGTSRSEIHSINITIERKNDEPPRMTLQPLRVQLSSGVVISNSSLSLQDLDTPDNELIFVLTKKPDHGHVLWRQTASEPLENGRVLVQGSTFTYQDILAGLVGYVPSVPGMVVDEFQFSLTDGLHVDTGRMKIYTELPASDTPHLAINQGLQLSAGSVARITEQHLKVTDIDSDDHQVMYIMKEDPGAGRLQMMKHGNLEQISIKGPIRSFTQADISQGHVEYSHGTGEPGGSFAFKFDVVDGEGNRLIDKSFSISILEDKSPPVITTNKGLVLDENSVKKITTLQLSATDQDSGPTELIYRITRQPQLGHLEHAASPGIQISSFTQADLTSRNVQYVHSSEAEKHSDAFSFTLSDGVSEVTQTFHITLHPVDDSLPVVQNLGMRVQEGMRKTITEFELKAVDADTEAESVTFTIVQPPRHGTIERTSNGQHFHLTSTFTMKDIYQNRVSYSHDGSNSLKDRFTFTVSDGTNPFFIIEEGGKEIMTAAPQPFRVDILPVDDGTPRIVTNLGLQWLEYMDGKATNLITKKELLTMDPDTEDAQLVYEITTGPKHGFVENKLQPGRAAATFTQEDVNLGLIRYVLHKEKIREMMDSFQFLVKDSKPNVVSDNVFHIQWSLISFKYTSYNVSEKAGSVSVTVQRTGNLNQYAIVLCRTEQGTASSSSRVSSQPGQQDYVEYAGQVQFDEREDTKSCTIVINDDDVFENVESFTVELSMPAYALLGEFTQAKVIINDTEDEPTLEFDKKIYWVNESAGFLFAPIERKGDASSIVSAICYTVPKSAMGSSLYALESGSDFKSRGMSAASRVIFGPGVTMSTCDVMLIDDSEYEEEEEFEIALADASDNARIGRVATAKVLISGPNDASTVSLGNTAFTVSEDAGTVKIPVIRHGTDLSTFASVWCATRPSDPASATPGVDYVPSSRKVEFGPGVIEQYCTLTILDDTQYPVIEGLETFVVFLSSAQGAELTKPFQAVIAINDTFQDVPSMQFAKDLLLVKEKEGVLHVPITRSGDLSYESSVRCYTQSHSAQVMEDFEERQNADSSRITFLKGDKVKNCTVYIHDDSMFEPEEQFRVYLGLPLGNHWSGARIGKNNMATITISNDEDAPTIEFEEAAYQVREPAGPDAIAILNIKVIRRGDQNRTSKVRCSTRDGSAQSGVDYYPKSRVLKFSPGVDHIFFKVEILSNEDREWHESFSLVLGPDDPVEAVLGDVTTATVTILDQEAAGSLILPAPPIVVTLADYDHVEEVTKEGVKKSPSPGYPLVCVTPCDPHFPRYAVMKERCSEAGINQTSVQFSWEVAAPTDGNGARSPFETITDNTPFTSVNHMVLDSIYFSRRFHVRCVAKAVDKVGHVGTPLRSNIVTIGTDSAICHTPVVAGTSRGFQAQSFIATLKYLDVKHKEHPNRIHISVQIPHQDGMLPLISTMPLHNLHFLLSESIYRHQHVCSNLVTTYDLRGISEAGFLDDVVYDSTALGPGYDRPFQFDPSVREPKTIQLYKHLNLKSCVWTFDAYYDMTELIDVCGGSVTADFQVRDSAQSFLTVHVPLYVSYIYVTAPRGWASLEHHTEMEFSFFYDTVLWRTGIQTDSVLSARLQIIRIYIREDGRLVIEFKTHAKFRGQFVMEHHTLPEVKSFVLTPDHLGGIEFDLQLLWSAQTFDSPHQLWRATSSYNRKDYSGEYTIYLIPCTVQPTQPWVDPGEKPLACTAHAPERFLIPIAFQQTNRPVPVVYSLNTEFQLCNNEKVFLMDPNTSDMSLAEMDYKGAFSKGQILYGRVLWNPEQNLNSAYKLQLEKVYLCTGKDGYVPFFDPTGTIYNEGPQYGCIQPNKHLKHRFLLLDRNQPEVTDKYFHDVPFEAHFASELPDFHVVSNMPGVDGFTLKVDALYKVEAGHQWYLQVIYIIGPDTISGPRVQRSLTAPLRRNRRDLVEPDGQLILDDSLIYDNEGDQVKNGTNMKSLNLEMQELAVAASLSQTGASIGSALAAIMLLLLVFLVACFINRKCQKQRKKKPAEDILEEYPLNTKVEVPKRHPDRVEKNVNRHYCTVRNVNILSEPEAAYTFKGAKVKRLNLEVRVHNNLQDGTEV

Target ORF information:

RefSeq Version	NM_025074.7
Organism	Homo sapiens(human)
Definition	Homo sapiens Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025074.7

ORF Insert Sequence:

ATGGGTGTCC TCAAAGTGTG GCTCGGGCTG GCCCTAGCGT TGGCGGAATT TGCAGTATTG 
CCTCATCATT CCGAAGGTGC TTGTGTCTAT CAGGATTCCT TGTTGGCGGA TGCCACAATT 
TGGAAGCCCG ATTCATGCCA GAGCTGCCGT TGCCATGGTG ATATTGTTAT CTGCAAACCT 
GCTGTTTGCA GAAACCCTCA ATGTGCCTTT GAGAAGGGAG AAGTGCTTCA AATAGCTGCC 
AACCAATGCT GTCCTGAGTG TGTTTTGAGG ACTCCAGGAT CTTGCCATCA TGAAAAGAAA 
ATCCATGAGC ATGGGACAGA ATGGGCCTCT TCTCCATGTA GTGTGTGCTC TTGCAATCAT 
GGGGAAGTCC GATGTACCCC CCAACCATGC CCACCGCTGT CATGTGGACA CCAGGAGCTG 
GCATTCATCC CTGAAGGAAG CTGCTGCCCA GTTTGTGTGG GCCTTGGGAA ACCCTGTTCC 
TATGAAGGCC ATGTGTTTCA GGATGGGGAG GACTGGCGGC TGAGCCGGTG TGCCAAATGT 
CTGTGTAGAA ATGGGGTTGC CCAGTGCTTC ACAGCTCAGT GTCAGCCTCT ATTTTGTAAC 
CAGGATGAGA CTGTAGTCCG AGTCCCTGGA AAATGTTGCC CGCAGTGCTC TGCAAGATCC 
TGCTCTGCAG CTGGCCAAGT ATACGAGCAT GGTGAGCAGT GGAGCGAAAA TGCCTGCACC 
ACGTGTATAT GTGACCGGGG TGAGGTCAGG TGTCACAAGC AGGCCTGCCT GCCCCTGAGA 
TGCGGAAAGG GTCAGAGCAG GGCTCGGCGT CATGGGCAAT GCTGTGAGGA ATGTGTGTCT 
CCTGCCGGGA GCTGCTCCTA TGATGGAGTT GTGCGGTACC AGGACGAAAT GTGGAAGGGC 
TCGGCCTGTG AGTTCTGCAT GTGTGATCAT GGCCAAGTGA CCTGCCAGAC TGGAGAGTGT 
GCCAAAGTGG AGTGTGCCCG GGATGAAGAA TTAATTCACT TAGATGGAAA GTGTTGTCCT 
GAATGCATTT CAAGGAATGG TTATTGTGTT TATGAAGAAA CTGGAGAATT TATGTCATCA 
AATGCTAGTG AAGTTAAACG TATTCCAGAG GGAGAGAAGT GGGAAGATGG CCCTTGCAAG 
GTGTGTGAGT GCCGAGGGGC TCAGGTAACT TGCTACGAGC CCTCTTGCCC ACCATGTCCA 
GTGGGCACAC TGGCCTTAGA GGTGAAGGGA CAGTGCTGTC CAGACTGCAC ATCAGTTCAT 
TGCCATCCAG ATTGTTTGAC ATGCTCTCAG TCTCCAGACC ACTGTGACCT CTGCCAAGAT 
CCTACCAAGT TACTGCAGAA TGGATGGTGT GTGCACAGCT GTGGACTGGG TTTTTACCAA 
GCTGGCAGTC TCTGTTTAGC CTGCCAGCCC CAGTGCTCCA CGTGTACCAG TGGGCTGGAG 
TGCTCATCCT GCCAGCCTCC CCTGCTGATG CGGCACGGGC AGTGTGTGCC TACCTGTGGG 
GACGGCTTCT ACCAAGATCG CCATTCCTGT GCAGTCTGCC ATGAGTCCTG TGCAGGTTGC 
TGGGGCCCAA CGGAGAAGCA CTGCTTGGCC TGCAGAGATC CCCTCCACGT GCTGAGAGAT 
GGCGGCTGTG AGAGCAGCTG TGGAAAAGGC TTCTACAACA GGCAGGGCAC CTGTAGCGCT 
TGTGACCAAT CCTGTGACAG TTGTGGCCCC AGTAGCCCCA GGTGTCTTAC CTGTACTGAG 
AAGACAGTGC TGCATGATGG GAAATGCATG TCTGAATGCC CTGGCGGGTA CTATGCTGAT 
GCCACTGGCA GGTGCAAAGT TTGTCATAAC TCATGTGCCA GCTGCTCTGG GCCCACACCC 
TCTCACTGTA CAGCCTGCAG CCCCCCCAAG GCTCTGCGTC AAGGCCACTG TCTGCCCCGC 
TGTGGAGAGG GTTTCTACTC TGACCATGGA GTCTGCAAAG CCTGTCACTC CTCCTGCCTG 
GCTTGTATGG GTCCCGCACC CTCTCACTGT ACTGGGTGTA AGAAGCCAGA GGAAGGACTG 
CAAGTGGAGC AGCTGTCTGA CGTGGGCATC CCCTCTGGCG AGTGTCTAGC CCAGTGTAGA 
GCCCATTTTT ACTTGGAGAG CACTGGCATA TGTGAAGCTT GCCACCAGTC CTGTTTCAGA 
TGTGCAGGGA AAAGCCCACA TAACTGCACA GACTGTGGGC CTTCCCATGT GCTGTTGGAT 
GGGCAGTGCC TCTCCCAGTG CCCAGATGGC TACTTTCACC AGGAAGGTAG TTGCACAGAG 
TGTCACCCAA CCTGCAGGCA GTGTCATGGG CCGTTGGAGT CTGACTGCAT CTCCTGTTAC 
CCTCACATCT CTCTTACCAA TGGTAACTGC AGGACCAGCT GCAGGGAAGA GCAGTTCCTC 
AACCTCGTGG GATACTGTGC TGACTGCCAT CACCTGTGCC AGCACTGTGC AGCTGATCTC 
CACAACACTG GGAGCATCTG CCTCAGGTGC CAGAATGCCC ACTACCTGCT GCTCGGGGAC 
CACTGTGTTC CTGACTGCCC TTCAGGATAC TATGCAGAGA GAGGAGCTTG TAAAAAATGC 
CACTCCTCCT GCAGAACCTG CCAGGGCAGA GGACCTTTCT CCTGCTCCTC ATGTGACACC 
AACCTCGTGC TGTCCCACAC TGGCACCTGC AGCACCACCT GCTTCCCTGG GCACTATCTT 
GATGACAATC ATGTTTGCCA GCCATGCAAC ACACACTGTG GAAGCTGTGA TTCACAGGCC 
AGCTGTACCT CCTGCCGAGA TCCAAACAAG GTTCTGCTCT TTGGGGAATG TCAATACGAG 
AGCTGCGCCC CACAGTACTA TCTTGACTTC TCCACCAACA CGTGCAAAGA GTGTGATTGG 
AGCTGCAGTG CATGCAGTGG GCCCCTGAAA ACAGACTGCC TGCAGTGCAT GGATGGCTAT 
GTTCTCCAGG ATGGGGCCTG CGTGGAGCAG TGCTTGTCAT CATTTTACCA GGACTCGGGC 
CTCTGCAAGA ACTGTGACAG CTACTGTCTC CAGTGCCAAG GTCCCCATGA GTGTACCCGC 
TGCAAAGGGC CATTTCTCCT CTTGGAAGCC CAGTGTGTCC AGGAATGTGG GAAGGGGTAC 
TTTGCAGATC ATGCAAAGCA CAAATGCACA GCCTGCCCTC AGGGGTGCTT GCAGTGCAGC 
CACAGGGACC GTTGTCACCT CTGTGACCAT GGGTTCTTTC TGAAGAGTGG CCTCTGTGTT 
TACAACTGTG TTCCTGGCTT TTCTGTCCAC ACCTCTAATG AAACATGTTC TGGCAAAATA 
CACACCCCTA GTCTTCATGT GAATGGTTCC CTGATCCTCC CAATTGGTTC AATAAAGCCA 
CTGGATTTTT CCCTCCTGAA TGTCCAAGAC CAGGAGGGTA GGGTCGAAGA TCTCCTATTT 
CATGTTGTGA GCACTCCCAC CAATGGTCAG CTAGTGCTCT CAAGAAATGG AAAAGAGGTT 
CAGCTGGACA AGGCTGGCCG TTTTAGCTGG AAAGATGTGA ACGAGAAGAA AGTGCGTTTT 
GTGCACAGCA AAGAAAAACT CAGGAAAGGT TACCTTTTCC TGAAAATTAG TGACCAGCAG 
TTCTTCTCTG AGCCACAGCT GATCAACATA CAAGCATTTT CAACACAGGC CCCCTATGTG 
CTGAGAAATG AAGTTCTCCA CATTAGCAGA GGAGAGAGGG CAACCATCAC CACCCAGATG 
CTTGACATCC GAGATGATGA CAACCCACAG GATGTGGTCA TTGAAATAAT CGATCCTCCA 
CTTCATGGCC AATTGCTTCA GACACTTCAG TCCCCGGCAA CCCCTATCTA TCAATTCCAG 
CTGGATGAAC TCTCTAGAGG CCTTCTCCAC TATGCTCATG ATGGTTCAGA CAGCACATCC 
GATGTTGCAG TCTTGCAGGC CAATGATGGA CACTCCTTCC ATAATATACT GTTCCAAGTG 
AAGACCGTGC CTCAGAATGA CAGGGGTCTT CAGCTTGTGG CTAATTCGAT GGTGTGGGTT 
CCAGAAGGGG GGATGCTGCA GATCACCAAC AGAATCTTAC AGGCCGAGGC TCCTGGTGCC 
AGTGCTGAAG AAATCATCTA CAAGATTACA CAAGACTACC CCCAGTTTGG GGAGGTGGTC 
CTTCTAGTGA ATATGCCTGC AGACAGCCCT GCAGATGAAG GGCAGCACCT GCCTGATGGG 
AGGACAGCTA CCCCCACCAG CACCTTCACC CAGCAGGACA TCAATGAAGG CATCGTATGG 
TACAGGCACT CAGGAGCCCC AGCCCAGAGC GACTCCTTCC GCTTCGAGGT GTCCAGTGCC 
TCCAATGCCC AGACCCGCCT GGAGAGCCAC ATGTTCAACA TCGCGATCTT ACCACAGACA 
CCTGAAGCAC CTAAAGTGTC TCTGGAAGCA TCTCTCCATA TGACTGCTAG AGAAGATGGC 
CTGACTGTTA TTCAGCCTCA TTCCCTCTCC TTCATAAACT CTGAGAAGCC AAGTGGAAAG 
ATTGTCTACA ACATCACTCT ACCTCTGCAT CCAAATCAAG GTATCATCGA GCACCGGGAC 
CACCCTCACT CTCCTATCCG GTATTTCACG CAAGAGGATA TTAACCAGGG CAAAGTCATG 
TACCGCCCTC CCCCGGCAGC ACCCCACCTC CAGGAGCTCA TGGCCTTCTC GTTCGCTGGT 
CTCCCAGAAT CAGTGAAATT CCACTTCACA GTTTCAGATG GAGAACACAC AAGTCCGGAG 
ATGGTCCTCA CCATTCACTT ACTTCCCAGT GATCAGCAAC TGCCAGTGTT CCAGGTCACA 
GCTCCACGGC TGGCGGTCAG CCCAGGAGGC AGCACTTCTG TAGGACTTCA GGTGGTAGTA 
AGAGATGCTG AGACAGCGCC CAAAGAACTC TTCTTTGAGC TTCGGAGACC TCCACAGCAT 
GGTGTGCTTC TTAAGCATAC AGCTGAGTTC CGAAGGCCGA TGGCCACAGG TGACACTTTC 
ACCTATGAGG ATGTTGAGAA AAATGCTCTA CAGTATATAC ATGATGGTTC CTCTACCCGG 
GAAGACAGCA TGGAGATCTC AGTCACAGAT GGCCTCACAG TGACAATGCT GGAGGTGAGA 
GTAGAGGTGT CCCTGTCAGA AGACCGAGGG CCTCGACTGG CTGCTGGCTC CTCTCTGAGC 
ATTACTGTTG CCAGTAAAAG CACAGCCATA ATCACTAGGT CACACCTTGC TTACGTGGAT 
GATTCTTCCC CCGACCCAGA GATCTGGATT CAGTTAAATT ATCTGCCCTC ATATGGTACT 
CTCTTAAGAA TCTCAGGATC TGAGGTGGAA GAGCTCTCAG AAGTTTCCAA TTTCACAATG 
GAAGACATCA ATAACAAGAA AATCAGATAC TCAGCTGTGT TTGAAACTGA TGGTCATCTG 
GTTACTGATA GCTTCTATTT CTCTGTCTCT GACATGGACC ACAACCATCT GGATAATCAG 
ATATTTACCA TCATGATCAC TCCTGCTGAA AATCCACCTC CAGTCATTGC TTTTGCTGAC 
CTTATCACGG TTGATGAGGG AGGGAGAGCA CCACTCTCAT TTCACCATTT TTTTGCTACT 
GATGATGATG ACAACCTCCA GAGAGATGCC ATCATTAAAC TAAGTGCTCT GCCCAAATAT 
GGCTGCATTG AGAACACAGG AACAGGTGAT CGTTTTGGCC CTGAAACTGC CAGTGACCTA 
GAGGCATCAT TTCCTATTCA AGACGTCCTG GAAAACTACA TTTACTACTT TCAGAGTGTT 
CATGAAAGCA TTGAGCCAAC CCATGATATT TTTAGTTTTT ATGTGAGTGA TGGAACCAGT 
CGTTCAGAAA TTCACAGCAT CAATATCACC ATTGAGAGGA AGAACGATGA GCCTCCCAGG 
ATGACCTTGC AGCCCCTCAG AGTGCAGCTG AGCTCGGGAG TGGTGATAAG CAATTCTTCT 
TTGAGCCTGC AAGACCTGGA CACCCCAGAT AATGAGCTCA TTTTTGTATT GACAAAAAAG 
CCTGACCACG GTCATGTACT CTGGAGGCAA ACTGCTTCTG AGCCTCTGGA GAATGGGAGA 
GTTTTAGTCC AGGGCTCAAC CTTCACCTAC CAGGATATCC TAGCTGGGCT GGTTGGGTAT 
GTGCCTAGTG TCCCTGGCAT GGTCGTGGAT GAGTTCCAGT TCTCCCTCAC TGATGGCCTC 
CACGTGGACA CAGGGAGGAT GAAGATCTAC ACAGAACTGC CTGCAAGTGA CACACCTCAC 
TTGGCTATAA ACCAAGGCCT ACAGCTCTCA GCAGGGTCTG TAGCACGCAT CACAGAACAG 
CACTTGAAAG TGACAGATAT TGACTCAGAT GACCATCAGG TTATGTACAT CATGAAGGAA 
GATCCTGGTG CAGGGCGCCT GCAGATGATG AAGCATGGCA ACCTGGAGCA AATTTCTATT 
AAAGGCCCCA TCCGAAGTTT CACCCAGGCA GACATTAGCC AAGGCCACGT AGAATATAGT 
CATGGAACAG GAGAACCTGG AGGGAGCTTT GCTTTTAAAT TTGATGTGGT TGATGGAGAA 
GGCAACAGAT TGATTGACAA GTCATTTTCC ATCAGCATTC TAGAAGACAA ATCCCCACCA 
GTCATCACCA CCAATAAAGG ACTGGTCTTG GATGAAAACT CAGTGAAGAA AATCACCACC 
CTGCAGCTGT CTGCCACTGA CCAGGACAGT GGGCCTACAG AATTGATCTA CAGAATCACC 
AGACAGCCCC AGCTGGGCCA CTTGGAACAT GCAGCATCAC CAGGTATCCA GATTAGTTCC 
TTTACTCAAG CTGATCTGAC TTCACGAAAT GTTCAGTATG TCCATTCTAG TGAGGCTGAG 
AAACATTCAG ATGCCTTCAG CTTTACACTG TCTGATGGAG TCAGTGAGGT GACTCAGACT 
TTCCATATCA CTCTTCACCC TGTCGATGAT TCGCTGCCCG TCGTACAGAA CTTAGGAATG 
CGGGTGCAGG AGGGCATGAG GAAGACCATC ACAGAGTTTG AGCTTAAGGC GGTGGATGCT 
GACACAGAGG CCGAGTCTGT CACATTCACC ATCGTGCAGC CTCCACGCCA TGGCACCATC 
GAGCGAACCA GCAATGGGCA GCATTTCCAC CTCACCTCCA CCTTCACCAT GAAAGATATC 
TACCAGAACC GGGTCAGCTA CAGCCATGAC GGCAGTAACT CCCTCAAGGA CCGGTTCACC 
TTCACTGTTT CTGATGGGAC AAACCCCTTC TTTATCATTG AGGAAGGGGG AAAAGAGATT 
ATGACAGCAG CACCTCAGCC GTTCCGAGTA GACATCCTCC CGGTAGATGA TGGCACGCCT 
AGAATTGTCA CCAACCTGGG ACTCCAGTGG CTGGAATACA TGGATGGCAA GGCAACCAAC 
CTGATCACCA AGAAGGAACT GCTGACCATG GACCCAGACA CCGAGGACGC GCAGCTTGTC 
TATGAGATAA CGACGGGCCC TAAGCATGGC TTTGTGGAGA ACAAGCTGCA GCCTGGCAGA 
GCTGCTGCCA CTTTCACCCA GGAGGATGTG AACTTGGGGT TGATTCGTTA TGTGTTGCAC 
AAGGAGAAGA TCCGTGAGAT GATGGATAGT TTTCAGTTTC TGGTGAAAGA CAGTAAACCC 
AATGTGGTCA GCGACAATGT CTTCCATATC CAGTGGTCAC TCATCAGCTT TAAATATACC 
AGCTACAATG TCAGTGAGAA GGCAGGGTCT GTCAGTGTCA CGGTGCAGAG GACTGGGAAC 
CTGAACCAAT ATGCCATCGT CCTGTGTCGC ACCGAGCAAG GCACCGCCAG CTCCAGCTCC 
AGGGTCAGCT CCCAACCTGG GCAACAGGAC TATGTAGAGT ATGCTGGCCA GGTCCAGTTT 
GATGAGCGAG AGGACACCAA GTCCTGCACC ATTGTCATCA ACGATGATGA CGTGTTTGAA 
AATGTTGAGA GTTTCACTGT GGAGCTCAGC ATGCCAGCTT ATGCCCTGTT AGGGGAATTC 
ACCCAGGCGA AGGTCATTAT CAACGATACC GAGGATGAAC CCACATTAGA GTTTGACAAG 
AAGATCTACT GGGTTAACGA GAGCGCTGGT TTTCTGTTTG CACCTATTGA AAGAAAAGGA 
GATGCAAGCA GCATTGTATC TGCAATTTGC TACACAGTCC CTAAGTCAGC TATGGGAAGT 
AGCCTCTATG CTCTAGAATC AGGCTCTGAT TTTAAATCTA GAGGGATGTC TGCCGCGAGT 
CGTGTGATAT TCGGGCCTGG TGTGACCATG TCCACCTGTG ATGTCATGCT TATTGATGAC 
AGCGAGTATG AAGAGGAAGA AGAGTTTGAG ATTGCCTTGG CAGATGCCTC TGACAATGCC 
CGCATTGGAA GGGTGGCGAC AGCCAAGGTG CTCATTAGTG GTCCCAACGA TGCCTCGACT 
GTGTCCCTGG GCAACACGGC TTTCACTGTC AGTGAGGACG CAGGCACAGT AAAGATTCCA 
GTTATCCGCC ATGGTACTGA CCTCTCTACT TTCGCATCTG TCTGGTGTGC AACGCGGCCC 
TCAGACCCAG CTTCTGCCAC ACCAGGAGTT GACTACGTTC CCAGCTCTCG GAAGGTGGAA 
TTTGGGCCTG GTGTCATTGA ACAGTATTGC ACCTTGACTA TCTTGGATGA CACTCAGTAT 
CCGGTAATTG AAGGACTGGA GACATTTGTG GTTTTCCTCA GCTCAGCACA AGGAGCCGAA 
CTGACCAAAC CCTTCCAGGC AGTCATTGCA ATTAATGACA CATTCCAAGA TGTGCCCAGC 
ATGCAGTTTG CCAAGGATTT GCTCCTAGTG AAGGAGAAGG AGGGTGTCCT GCATGTCCCT 
ATCACTCGGA GCGGAGACCT GAGCTATGAG TCATCAGTGA GGTGCTATAC TCAGAGCCAT 
TCCGCTCAGG TCATGGAGGA CTTTGAGGAG AGACAAAATG CAGACTCTTC ACGGATTACA 
TTTCTCAAAG GGGACAAAGT GAAGAACTGT ACGGTCTATA TCCACGATGA CTCCATGTTT 
GAGCCAGAGG AACAGTTCAG GGTCTACCTC GGCCTTCCTC TTGGAAACCA CTGGAGTGGA 
GCTAGAATTG GAAAGAATAA CATGGCCACC ATCACCATAT CCAATGATGA AGATGCCCCC 
ACCATTGAGT TTGAAGAAGC TGCATACCAA GTCCGGGAAC CCGCAGGCCC AGATGCCATT 
GCGATTCTGA ACATCAAGGT GATCCGCAGA GGGGATCAGA ACAGGACCTC CAAGGTTCGC 
TGCAGCACGC GGGATGGCTC TGCCCAGTCT GGTGTGGATT ATTACCCAAA GAGCCGAGTC 
TTGAAGTTCA GTCCCGGTGT GGATCATATC TTTTTTAAAG TTGAGATCCT GTCCAATGAA 
GACCGGGAAT GGCATGAATC TTTCTCACTA GTCCTTGGCC CAGATGACCC AGTGGAAGCA 
GTTCTTGGGG ATGTGACTAC TGCCACGGTG ACAATTCTAG ACCAGGAGGC AGCAGGGAGC 
CTCATATTGC CAGCACCACC CATTGTGGTC ACACTTGCTG ACTATGACCA TGTGGAAGAA 
GTTACCAAGG AAGGAGTCAA GAAATCCCCC TCCCCAGGCT ACCCACTGGT CTGTGTCACC 
CCCTGCGACC CTCATTTCCC CAGATACGCT GTCATGAAGG AGCGCTGCAG TGAGGCCGGC 
ATCAACCAGA CATCTGTGCA GTTCAGCTGG GAAGTGGCTG CCCCCACTGA TGGCAATGGG 
GCCCGGTCTC CCTTTGAAAC CATCACTGAC AACACACCAT TCACCAGTGT CAACCACATG 
GTCCTGGACA GCATTTACTT CAGCCGGAGG TTCCATGTGC GTTGTGTGGC CAAGGCTGTG 
GACAAGGTGG GCCATGTGGG GACCCCCTTA AGGAGCAACA TTGTTACCAT TGGAACAGAC 
AGTGCTATCT GCCACACACC AGTGGTGGCT GGGACATCCA GAGGCTTCCA GGCTCAGTCC 
TTCATCGCAA CCTTGAAATA CCTGGATGTC AAACATAAGG AGCATCCGAA CAGAATCCAC 
ATTTCGGTGC AGATCCCACA CCAGGATGGA ATGCTGCCCC TTATCTCCAC CATGCCGTTG 
CACAACTTAC ATTTTCTACT GTCTGAGTCC ATCTACAGAC ACCAGCACGT CTGCTCCAAT 
TTAGTTACCA CCTATGACCT GAGAGGCATC TCAGAGGCAG GGTTCCTGGA TGATGTGGTC 
TATGATAGCA CTGCCCTGGG GCCTGGCTAC GATCGCCCCT TCCAGTTTGA CCCCAGCGTG 
CGAGAGCCGA AGACCATCCA GCTCTACAAA CACCTGAACC TGAAGAGCTG CGTGTGGACC 
TTTGATGCTT ATTATGACAT GACTGAGCTG ATTGACGTCT GTGGGGGCTC TGTAACCGCT 
GACTTCCAGG TGAGGGACTC TGCCCAGTCC TTCTTGACAG TGCACGTGCC TCTATATGTG 
TCCTACATCT ATGTGACAGC CCCCAGGGGC TGGGCCTCCT TGGAGCACCA CACCGAGATG 
GAGTTTTCTT TCTTCTATGA CACTGTTCTC TGGAGAACAG GAATCCAGAC AGACAGCGTG 
CTCTCTGCAA GGCTTCAGAT AATAAGAATC TACATTCGAG AGGATGGCCG TCTTGTCATT 
GAATTCAAGA CCCATGCCAA ATTCAGAGGA CAGTTTGTGA TGGAGCATCA CACTCTCCCA 
GAAGTGAAAT CTTTCGTATT GACTCCAGAC CACCTAGGAG GAATTGAATT TGACTTGCAG 
CTATTATGGA GCGCTCAGAC TTTTGATTCT CCACATCAAC TCTGGAGAGC CACAAGCTCT 
TATAACAGGA AGGACTACTC AGGAGAGTAC ACCATCTACC TGATCCCTTG CACAGTGCAG 
CCCACACAGC CATGGGTTGA CCCAGGAGAG AAGCCTTTGG CCTGCACTGC ACATGCCCCA 
GAAAGATTCC TGATACCCAT TGCATTCCAG CAGACCAACC GCCCTGTGCC AGTTGTGTAT 
TCACTTAACA CTGAATTTCA GCTCTGCAAT AATGAGAAGG TGTTCCTAAT GGATCCCAAT 
ACATCTGATA TGTCACTAGC AGAAATGGAT TACAAAGGAG CCTTTTCAAA AGGTCAAATC 
CTTTATGGCC GAGTACTTTG GAATCCAGAA CAAAATCTTA ATTCTGCTTA CAAACTCCAG 
CTGGAGAAAG TCTATCTTTG TACGGGCAAG GATGGTTATG TGCCTTTCTT TGATCCCACG 
GGGACAATCT ACAATGAAGG GCCCCAGTAT GGATGCATTC AGCCAAACAA ACACCTAAAA 
CACAGATTCC TGCTGTTGGA CCGCAATCAG CCAGAGGTAA CTGATAAGTA CTTCCATGAT 
GTGCCTTTTG AGGCTCACTT TGCCTCTGAG TTGCCTGATT TCCATGTGGT CAGTAACATG 
CCAGGTGTGG ATGGATTTAC TCTAAAAGTA GATGCACTCT ATAAGGTGGA AGCAGGACAC 
CAGTGGTATC TCCAGGTCAT CTACATCATT GGCCCTGACA CCATCTCAGG GCCCCGGGTC 
CAGCGCTCTC TCACAGCTCC ACTCAGACGC AACCGAAGGG ACCTGGTAGA GCCCGATGGC 
CAGCTGATCC TTGATGATTC CCTCATCTAT GACAATGAAG GAGACCAAGT CAAGAATGGC 
ACCAATATGA AGTCCCTGAA TCTGGAGATG CAAGAGTTGG CGGTAGCTGC GTCCCTGTCA 
CAGACTGGGG CGTCCATTGG CAGTGCCCTG GCTGCAATCA TGCTTCTACT TCTGGTGTTT 
TTGGTGGCTT GTTTTATCAA CAGGAAATGC CAGAAACAGA GGAAGAAGAA GCCCGCAGAG 
GACATTTTGG AAGAATATCC TCTGAATACC AAGGTAGAAG TGCCCAAGAG GCACCCGGAC 
CGGGTGGAGA AGAACGTGAA TAGACACTAC TGCACTGTGC GGAACGTCAA CATCCTGAGT 
GAGCCTGAGG CGGCTTACAC GTTCAAAGGT GCTAAAGTCA AAAGACTGAA TCTAGAAGTC 
AGAGTTCACA ACAATTTACA AGATGGAACA GAAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FRAS1 ( NM_025074.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_079350.5 Homo sapiens Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant 1, mRNA.

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CloneID	OHu28517
Clone ID Related Accession (Same CDS sequence)	NM_025074.7 , NM_025074.6
Accession Version	NM_025074.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-17
Organism	Homo sapiens(human)
Product	extracellular matrix protein FRAS1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA725113.1, DA881824.1, BC131820.1, AC093886.2, AB040933.2, AC104808.4, BX647949.1 and BX647420.1. This sequence is a reference standard in the RefSeqGene project. On Aug 18, 2009 this sequence version replaced NM_025074.5. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGTGTCC TCAAAGTGTG GCTCGGGCTG GCCCTAGCGT TGGCGGAATT TGCAGTATTG 
CCTCATCATT CCGAAGGTGC TTGTGTCTAT CAGGATTCCT TGTTGGCGGA TGCCACAATT 
TGGAAGCCCG ATTCATGCCA GAGCTGCCGT TGCCATGGTG ATATTGTTAT CTGCAAACCT 
GCTGTTTGCA GAAACCCTCA ATGTGCCTTT GAGAAGGGAG AAGTGCTTCA AATAGCTGCC 
AACCAATGCT GTCCTGAGTG TGTTTTGAGG ACTCCAGGAT CTTGCCATCA TGAAAAGAAA 
ATCCATGAGC ATGGGACAGA ATGGGCCTCT TCTCCATGTA GTGTGTGCTC TTGCAATCAT 
GGGGAAGTCC GATGTACCCC CCAACCATGC CCACCGCTGT CATGTGGACA CCAGGAGCTG 
GCATTCATCC CTGAAGGAAG CTGCTGCCCA GTTTGTGTGG GCCTTGGGAA ACCCTGTTCC 
TATGAAGGCC ATGTGTTTCA GGATGGGGAG GACTGGCGGC TGAGCCGGTG TGCCAAATGT 
CTGTGTAGAA ATGGGGTTGC CCAGTGCTTC ACAGCTCAGT GTCAGCCTCT ATTTTGTAAC 
CAGGATGAGA CTGTAGTCCG AGTCCCTGGA AAATGTTGCC CGCAGTGCTC TGCAAGATCC 
TGCTCTGCAG CTGGCCAAGT ATACGAGCAT GGTGAGCAGT GGAGCGAAAA TGCCTGCACC 
ACGTGTATAT GTGACCGGGG TGAGGTCAGG TGTCACAAGC AGGCCTGCCT GCCCCTGAGA 
TGCGGAAAGG GTCAGAGCAG GGCTCGGCGT CATGGGCAAT GCTGTGAGGA ATGTGTGTCT 
CCTGCCGGGA GCTGCTCCTA TGATGGAGTT GTGCGGTACC AGGACGAAAT GTGGAAGGGC 
TCGGCCTGTG AGTTCTGCAT GTGTGATCAT GGCCAAGTGA CCTGCCAGAC TGGAGAGTGT 
GCCAAAGTGG AGTGTGCCCG GGATGAAGAA TTAATTCACT TAGATGGAAA GTGTTGTCCT 
GAATGCATTT CAAGGAATGG TTATTGTGTT TATGAAGAAA CTGGAGAATT TATGTCATCA 
AATGCTAGTG AAGTTAAACG TATTCCAGAG GGAGAGAAGT GGGAAGATGG CCCTTGCAAG 
GTGTGTGAGT GCCGAGGGGC TCAGGTAACT TGCTACGAGC CCTCTTGCCC ACCATGTCCA 
GTGGGCACAC TGGCCTTAGA GGTGAAGGGA CAGTGCTGTC CAGACTGCAC ATCAGTTCAT 
TGCCATCCAG ATTGTTTGAC ATGCTCTCAG TCTCCAGACC ACTGTGACCT CTGCCAAGAT 
CCTACCAAGT TACTGCAGAA TGGATGGTGT GTGCACAGCT GTGGACTGGG TTTTTACCAA 
GCTGGCAGTC TCTGTTTAGC CTGCCAGCCC CAGTGCTCCA CGTGTACCAG TGGGCTGGAG 
TGCTCATCCT GCCAGCCTCC CCTGCTGATG CGGCACGGGC AGTGTGTGCC TACCTGTGGG 
GACGGCTTCT ACCAAGATCG CCATTCCTGT GCAGTCTGCC ATGAGTCCTG TGCAGGTTGC 
TGGGGCCCAA CGGAGAAGCA CTGCTTGGCC TGCAGAGATC CCCTCCACGT GCTGAGAGAT 
GGCGGCTGTG AGAGCAGCTG TGGAAAAGGC TTCTACAACA GGCAGGGCAC CTGTAGCGCT 
TGTGACCAAT CCTGTGACAG TTGTGGCCCC AGTAGCCCCA GGTGTCTTAC CTGTACTGAG 
AAGACAGTGC TGCATGATGG GAAATGCATG TCTGAATGCC CTGGCGGGTA CTATGCTGAT 
GCCACTGGCA GGTGCAAAGT TTGTCATAAC TCATGTGCCA GCTGCTCTGG GCCCACACCC 
TCTCACTGTA CAGCCTGCAG CCCCCCCAAG GCTCTGCGTC AAGGCCACTG TCTGCCCCGC 
TGTGGAGAGG GTTTCTACTC TGACCATGGA GTCTGCAAAG CCTGTCACTC CTCCTGCCTG 
GCTTGTATGG GTCCCGCACC CTCTCACTGT ACTGGGTGTA AGAAGCCAGA GGAAGGACTG 
CAAGTGGAGC AGCTGTCTGA CGTGGGCATC CCCTCTGGCG AGTGTCTAGC CCAGTGTAGA 
GCCCATTTTT ACTTGGAGAG CACTGGCATA TGTGAAGCTT GCCACCAGTC CTGTTTCAGA 
TGTGCAGGGA AAAGCCCACA TAACTGCACA GACTGTGGGC CTTCCCATGT GCTGTTGGAT 
GGGCAGTGCC TCTCCCAGTG CCCAGATGGC TACTTTCACC AGGAAGGTAG TTGCACAGAG 
TGTCACCCAA CCTGCAGGCA GTGTCATGGG CCGTTGGAGT CTGACTGCAT CTCCTGTTAC 
CCTCACATCT CTCTTACCAA TGGTAACTGC AGGACCAGCT GCAGGGAAGA GCAGTTCCTC 
AACCTCGTGG GATACTGTGC TGACTGCCAT CACCTGTGCC AGCACTGTGC AGCTGATCTC 
CACAACACTG GGAGCATCTG CCTCAGGTGC CAGAATGCCC ACTACCTGCT GCTCGGGGAC 
CACTGTGTTC CTGACTGCCC TTCAGGATAC TATGCAGAGA GAGGAGCTTG TAAAAAATGC 
CACTCCTCCT GCAGAACCTG CCAGGGCAGA GGACCTTTCT CCTGCTCCTC ATGTGACACC 
AACCTCGTGC TGTCCCACAC TGGCACCTGC AGCACCACCT GCTTCCCTGG GCACTATCTT 
GATGACAATC ATGTTTGCCA GCCATGCAAC ACACACTGTG GAAGCTGTGA TTCACAGGCC 
AGCTGTACCT CCTGCCGAGA TCCAAACAAG GTTCTGCTCT TTGGGGAATG TCAATACGAG 
AGCTGCGCCC CACAGTACTA TCTTGACTTC TCCACCAACA CGTGCAAAGA GTGTGATTGG 
AGCTGCAGTG CATGCAGTGG GCCCCTGAAA ACAGACTGCC TGCAGTGCAT GGATGGCTAT 
GTTCTCCAGG ATGGGGCCTG CGTGGAGCAG TGCTTGTCAT CATTTTACCA GGACTCGGGC 
CTCTGCAAGA ACTGTGACAG CTACTGTCTC CAGTGCCAAG GTCCCCATGA GTGTACCCGC 
TGCAAAGGGC CATTTCTCCT CTTGGAAGCC CAGTGTGTCC AGGAATGTGG GAAGGGGTAC 
TTTGCAGATC ATGCAAAGCA CAAATGCACA GCCTGCCCTC AGGGGTGCTT GCAGTGCAGC 
CACAGGGACC GTTGTCACCT CTGTGACCAT GGGTTCTTTC TGAAGAGTGG CCTCTGTGTT 
TACAACTGTG TTCCTGGCTT TTCTGTCCAC ACCTCTAATG AAACATGTTC TGGCAAAATA 
CACACCCCTA GTCTTCATGT GAATGGTTCC CTGATCCTCC CAATTGGTTC AATAAAGCCA 
CTGGATTTTT CCCTCCTGAA TGTCCAAGAC CAGGAGGGTA GGGTCGAAGA TCTCCTATTT 
CATGTTGTGA GCACTCCCAC CAATGGTCAG CTAGTGCTCT CAAGAAATGG AAAAGAGGTT 
CAGCTGGACA AGGCTGGCCG TTTTAGCTGG AAAGATGTGA ACGAGAAGAA AGTGCGTTTT 
GTGCACAGCA AAGAAAAACT CAGGAAAGGT TACCTTTTCC TGAAAATTAG TGACCAGCAG 
TTCTTCTCTG AGCCACAGCT GATCAACATA CAAGCATTTT CAACACAGGC CCCCTATGTG 
CTGAGAAATG AAGTTCTCCA CATTAGCAGA GGAGAGAGGG CAACCATCAC CACCCAGATG 
CTTGACATCC GAGATGATGA CAACCCACAG GATGTGGTCA TTGAAATAAT CGATCCTCCA 
CTTCATGGCC AATTGCTTCA GACACTTCAG TCCCCGGCAA CCCCTATCTA TCAATTCCAG 
CTGGATGAAC TCTCTAGAGG CCTTCTCCAC TATGCTCATG ATGGTTCAGA CAGCACATCC 
GATGTTGCAG TCTTGCAGGC CAATGATGGA CACTCCTTCC ATAATATACT GTTCCAAGTG 
AAGACCGTGC CTCAGAATGA CAGGGGTCTT CAGCTTGTGG CTAATTCGAT GGTGTGGGTT 
CCAGAAGGGG GGATGCTGCA GATCACCAAC AGAATCTTAC AGGCCGAGGC TCCTGGTGCC 
AGTGCTGAAG AAATCATCTA CAAGATTACA CAAGACTACC CCCAGTTTGG GGAGGTGGTC 
CTTCTAGTGA ATATGCCTGC AGACAGCCCT GCAGATGAAG GGCAGCACCT GCCTGATGGG 
AGGACAGCTA CCCCCACCAG CACCTTCACC CAGCAGGACA TCAATGAAGG CATCGTATGG 
TACAGGCACT CAGGAGCCCC AGCCCAGAGC GACTCCTTCC GCTTCGAGGT GTCCAGTGCC 
TCCAATGCCC AGACCCGCCT GGAGAGCCAC ATGTTCAACA TCGCGATCTT ACCACAGACA 
CCTGAAGCAC CTAAAGTGTC TCTGGAAGCA TCTCTCCATA TGACTGCTAG AGAAGATGGC 
CTGACTGTTA TTCAGCCTCA TTCCCTCTCC TTCATAAACT CTGAGAAGCC AAGTGGAAAG 
ATTGTCTACA ACATCACTCT ACCTCTGCAT CCAAATCAAG GTATCATCGA GCACCGGGAC 
CACCCTCACT CTCCTATCCG GTATTTCACG CAAGAGGATA TTAACCAGGG CAAAGTCATG 
TACCGCCCTC CCCCGGCAGC ACCCCACCTC CAGGAGCTCA TGGCCTTCTC GTTCGCTGGT 
CTCCCAGAAT CAGTGAAATT CCACTTCACA GTTTCAGATG GAGAACACAC AAGTCCGGAG 
ATGGTCCTCA CCATTCACTT ACTTCCCAGT GATCAGCAAC TGCCAGTGTT CCAGGTCACA 
GCTCCACGGC TGGCGGTCAG CCCAGGAGGC AGCACTTCTG TAGGACTTCA GGTGGTAGTA 
AGAGATGCTG AGACAGCGCC CAAAGAACTC TTCTTTGAGC TTCGGAGACC TCCACAGCAT 
GGTGTGCTTC TTAAGCATAC AGCTGAGTTC CGAAGGCCGA TGGCCACAGG TGACACTTTC 
ACCTATGAGG ATGTTGAGAA AAATGCTCTA CAGTATATAC ATGATGGTTC CTCTACCCGG 
GAAGACAGCA TGGAGATCTC AGTCACAGAT GGCCTCACAG TGACAATGCT GGAGGTGAGA 
GTAGAGGTGT CCCTGTCAGA AGACCGAGGG CCTCGACTGG CTGCTGGCTC CTCTCTGAGC 
ATTACTGTTG CCAGTAAAAG CACAGCCATA ATCACTAGGT CACACCTTGC TTACGTGGAT 
GATTCTTCCC CCGACCCAGA GATCTGGATT CAGTTAAATT ATCTGCCCTC ATATGGTACT 
CTCTTAAGAA TCTCAGGATC TGAGGTGGAA GAGCTCTCAG AAGTTTCCAA TTTCACAATG 
GAAGACATCA ATAACAAGAA AATCAGATAC TCAGCTGTGT TTGAAACTGA TGGTCATCTG 
GTTACTGATA GCTTCTATTT CTCTGTCTCT GACATGGACC ACAACCATCT GGATAATCAG 
ATATTTACCA TCATGATCAC TCCTGCTGAA AATCCACCTC CAGTCATTGC TTTTGCTGAC 
CTTATCACGG TTGATGAGGG AGGGAGAGCA CCACTCTCAT TTCACCATTT TTTTGCTACT 
GATGATGATG ACAACCTCCA GAGAGATGCC ATCATTAAAC TAAGTGCTCT GCCCAAATAT 
GGCTGCATTG AGAACACAGG AACAGGTGAT CGTTTTGGCC CTGAAACTGC CAGTGACCTA 
GAGGCATCAT TTCCTATTCA AGACGTCCTG GAAAACTACA TTTACTACTT TCAGAGTGTT 
CATGAAAGCA TTGAGCCAAC CCATGATATT TTTAGTTTTT ATGTGAGTGA TGGAACCAGT 
CGTTCAGAAA TTCACAGCAT CAATATCACC ATTGAGAGGA AGAACGATGA GCCTCCCAGG 
ATGACCTTGC AGCCCCTCAG AGTGCAGCTG AGCTCGGGAG TGGTGATAAG CAATTCTTCT 
TTGAGCCTGC AAGACCTGGA CACCCCAGAT AATGAGCTCA TTTTTGTATT GACAAAAAAG 
CCTGACCACG GTCATGTACT CTGGAGGCAA ACTGCTTCTG AGCCTCTGGA GAATGGGAGA 
GTTTTAGTCC AGGGCTCAAC CTTCACCTAC CAGGATATCC TAGCTGGGCT GGTTGGGTAT 
GTGCCTAGTG TCCCTGGCAT GGTCGTGGAT GAGTTCCAGT TCTCCCTCAC TGATGGCCTC 
CACGTGGACA CAGGGAGGAT GAAGATCTAC ACAGAACTGC CTGCAAGTGA CACACCTCAC 
TTGGCTATAA ACCAAGGCCT ACAGCTCTCA GCAGGGTCTG TAGCACGCAT CACAGAACAG 
CACTTGAAAG TGACAGATAT TGACTCAGAT GACCATCAGG TTATGTACAT CATGAAGGAA 
GATCCTGGTG CAGGGCGCCT GCAGATGATG AAGCATGGCA ACCTGGAGCA AATTTCTATT 
AAAGGCCCCA TCCGAAGTTT CACCCAGGCA GACATTAGCC AAGGCCACGT AGAATATAGT 
CATGGAACAG GAGAACCTGG AGGGAGCTTT GCTTTTAAAT TTGATGTGGT TGATGGAGAA 
GGCAACAGAT TGATTGACAA GTCATTTTCC ATCAGCATTC TAGAAGACAA ATCCCCACCA 
GTCATCACCA CCAATAAAGG ACTGGTCTTG GATGAAAACT CAGTGAAGAA AATCACCACC 
CTGCAGCTGT CTGCCACTGA CCAGGACAGT GGGCCTACAG AATTGATCTA CAGAATCACC 
AGACAGCCCC AGCTGGGCCA CTTGGAACAT GCAGCATCAC CAGGTATCCA GATTAGTTCC 
TTTACTCAAG CTGATCTGAC TTCACGAAAT GTTCAGTATG TCCATTCTAG TGAGGCTGAG 
AAACATTCAG ATGCCTTCAG CTTTACACTG TCTGATGGAG TCAGTGAGGT GACTCAGACT 
TTCCATATCA CTCTTCACCC TGTCGATGAT TCGCTGCCCG TCGTACAGAA CTTAGGAATG 
CGGGTGCAGG AGGGCATGAG GAAGACCATC ACAGAGTTTG AGCTTAAGGC GGTGGATGCT 
GACACAGAGG CCGAGTCTGT CACATTCACC ATCGTGCAGC CTCCACGCCA TGGCACCATC 
GAGCGAACCA GCAATGGGCA GCATTTCCAC CTCACCTCCA CCTTCACCAT GAAAGATATC 
TACCAGAACC GGGTCAGCTA CAGCCATGAC GGCAGTAACT CCCTCAAGGA CCGGTTCACC 
TTCACTGTTT CTGATGGGAC AAACCCCTTC TTTATCATTG AGGAAGGGGG AAAAGAGATT 
ATGACAGCAG CACCTCAGCC GTTCCGAGTA GACATCCTCC CGGTAGATGA TGGCACGCCT 
AGAATTGTCA CCAACCTGGG ACTCCAGTGG CTGGAATACA TGGATGGCAA GGCAACCAAC 
CTGATCACCA AGAAGGAACT GCTGACCATG GACCCAGACA CCGAGGACGC GCAGCTTGTC 
TATGAGATAA CGACGGGCCC TAAGCATGGC TTTGTGGAGA ACAAGCTGCA GCCTGGCAGA 
GCTGCTGCCA CTTTCACCCA GGAGGATGTG AACTTGGGGT TGATTCGTTA TGTGTTGCAC 
AAGGAGAAGA TCCGTGAGAT GATGGATAGT TTTCAGTTTC TGGTGAAAGA CAGTAAACCC 
AATGTGGTCA GCGACAATGT CTTCCATATC CAGTGGTCAC TCATCAGCTT TAAATATACC 
AGCTACAATG TCAGTGAGAA GGCAGGGTCT GTCAGTGTCA CGGTGCAGAG GACTGGGAAC 
CTGAACCAAT ATGCCATCGT CCTGTGTCGC ACCGAGCAAG GCACCGCCAG CTCCAGCTCC 
AGGGTCAGCT CCCAACCTGG GCAACAGGAC TATGTAGAGT ATGCTGGCCA GGTCCAGTTT 
GATGAGCGAG AGGACACCAA GTCCTGCACC ATTGTCATCA ACGATGATGA CGTGTTTGAA 
AATGTTGAGA GTTTCACTGT GGAGCTCAGC ATGCCAGCTT ATGCCCTGTT AGGGGAATTC 
ACCCAGGCGA AGGTCATTAT CAACGATACC GAGGATGAAC CCACATTAGA GTTTGACAAG 
AAGATCTACT GGGTTAACGA GAGCGCTGGT TTTCTGTTTG CACCTATTGA AAGAAAAGGA 
GATGCAAGCA GCATTGTATC TGCAATTTGC TACACAGTCC CTAAGTCAGC TATGGGAAGT 
AGCCTCTATG CTCTAGAATC AGGCTCTGAT TTTAAATCTA GAGGGATGTC TGCCGCGAGT 
CGTGTGATAT TCGGGCCTGG TGTGACCATG TCCACCTGTG ATGTCATGCT TATTGATGAC 
AGCGAGTATG AAGAGGAAGA AGAGTTTGAG ATTGCCTTGG CAGATGCCTC TGACAATGCC 
CGCATTGGAA GGGTGGCGAC AGCCAAGGTG CTCATTAGTG GTCCCAACGA TGCCTCGACT 
GTGTCCCTGG GCAACACGGC TTTCACTGTC AGTGAGGACG CAGGCACAGT AAAGATTCCA 
GTTATCCGCC ATGGTACTGA CCTCTCTACT TTCGCATCTG TCTGGTGTGC AACGCGGCCC 
TCAGACCCAG CTTCTGCCAC ACCAGGAGTT GACTACGTTC CCAGCTCTCG GAAGGTGGAA 
TTTGGGCCTG GTGTCATTGA ACAGTATTGC ACCTTGACTA TCTTGGATGA CACTCAGTAT 
CCGGTAATTG AAGGACTGGA GACATTTGTG GTTTTCCTCA GCTCAGCACA AGGAGCCGAA 
CTGACCAAAC CCTTCCAGGC AGTCATTGCA ATTAATGACA CATTCCAAGA TGTGCCCAGC 
ATGCAGTTTG CCAAGGATTT GCTCCTAGTG AAGGAGAAGG AGGGTGTCCT GCATGTCCCT 
ATCACTCGGA GCGGAGACCT GAGCTATGAG TCATCAGTGA GGTGCTATAC TCAGAGCCAT 
TCCGCTCAGG TCATGGAGGA CTTTGAGGAG AGACAAAATG CAGACTCTTC ACGGATTACA 
TTTCTCAAAG GGGACAAAGT GAAGAACTGT ACGGTCTATA TCCACGATGA CTCCATGTTT 
GAGCCAGAGG AACAGTTCAG GGTCTACCTC GGCCTTCCTC TTGGAAACCA CTGGAGTGGA 
GCTAGAATTG GAAAGAATAA CATGGCCACC ATCACCATAT CCAATGATGA AGATGCCCCC 
ACCATTGAGT TTGAAGAAGC TGCATACCAA GTCCGGGAAC CCGCAGGCCC AGATGCCATT 
GCGATTCTGA ACATCAAGGT GATCCGCAGA GGGGATCAGA ACAGGACCTC CAAGGTTCGC 
TGCAGCACGC GGGATGGCTC TGCCCAGTCT GGTGTGGATT ATTACCCAAA GAGCCGAGTC 
TTGAAGTTCA GTCCCGGTGT GGATCATATC TTTTTTAAAG TTGAGATCCT GTCCAATGAA 
GACCGGGAAT GGCATGAATC TTTCTCACTA GTCCTTGGCC CAGATGACCC AGTGGAAGCA 
GTTCTTGGGG ATGTGACTAC TGCCACGGTG ACAATTCTAG ACCAGGAGGC AGCAGGGAGC 
CTCATATTGC CAGCACCACC CATTGTGGTC ACACTTGCTG ACTATGACCA TGTGGAAGAA 
GTTACCAAGG AAGGAGTCAA GAAATCCCCC TCCCCAGGCT ACCCACTGGT CTGTGTCACC 
CCCTGCGACC CTCATTTCCC CAGATACGCT GTCATGAAGG AGCGCTGCAG TGAGGCCGGC 
ATCAACCAGA CATCTGTGCA GTTCAGCTGG GAAGTGGCTG CCCCCACTGA TGGCAATGGG 
GCCCGGTCTC CCTTTGAAAC CATCACTGAC AACACACCAT TCACCAGTGT CAACCACATG 
GTCCTGGACA GCATTTACTT CAGCCGGAGG TTCCATGTGC GTTGTGTGGC CAAGGCTGTG 
GACAAGGTGG GCCATGTGGG GACCCCCTTA AGGAGCAACA TTGTTACCAT TGGAACAGAC 
AGTGCTATCT GCCACACACC AGTGGTGGCT GGGACATCCA GAGGCTTCCA GGCTCAGTCC 
TTCATCGCAA CCTTGAAATA CCTGGATGTC AAACATAAGG AGCATCCGAA CAGAATCCAC 
ATTTCGGTGC AGATCCCACA CCAGGATGGA ATGCTGCCCC TTATCTCCAC CATGCCGTTG 
CACAACTTAC ATTTTCTACT GTCTGAGTCC ATCTACAGAC ACCAGCACGT CTGCTCCAAT 
TTAGTTACCA CCTATGACCT GAGAGGCATC TCAGAGGCAG GGTTCCTGGA TGATGTGGTC 
TATGATAGCA CTGCCCTGGG GCCTGGCTAC GATCGCCCCT TCCAGTTTGA CCCCAGCGTG 
CGAGAGCCGA AGACCATCCA GCTCTACAAA CACCTGAACC TGAAGAGCTG CGTGTGGACC 
TTTGATGCTT ATTATGACAT GACTGAGCTG ATTGACGTCT GTGGGGGCTC TGTAACCGCT 
GACTTCCAGG TGAGGGACTC TGCCCAGTCC TTCTTGACAG TGCACGTGCC TCTATATGTG 
TCCTACATCT ATGTGACAGC CCCCAGGGGC TGGGCCTCCT TGGAGCACCA CACCGAGATG 
GAGTTTTCTT TCTTCTATGA CACTGTTCTC TGGAGAACAG GAATCCAGAC AGACAGCGTG 
CTCTCTGCAA GGCTTCAGAT AATAAGAATC TACATTCGAG AGGATGGCCG TCTTGTCATT 
GAATTCAAGA CCCATGCCAA ATTCAGAGGA CAGTTTGTGA TGGAGCATCA CACTCTCCCA 
GAAGTGAAAT CTTTCGTATT GACTCCAGAC CACCTAGGAG GAATTGAATT TGACTTGCAG 
CTATTATGGA GCGCTCAGAC TTTTGATTCT CCACATCAAC TCTGGAGAGC CACAAGCTCT 
TATAACAGGA AGGACTACTC AGGAGAGTAC ACCATCTACC TGATCCCTTG CACAGTGCAG 
CCCACACAGC CATGGGTTGA CCCAGGAGAG AAGCCTTTGG CCTGCACTGC ACATGCCCCA 
GAAAGATTCC TGATACCCAT TGCATTCCAG CAGACCAACC GCCCTGTGCC AGTTGTGTAT 
TCACTTAACA CTGAATTTCA GCTCTGCAAT AATGAGAAGG TGTTCCTAAT GGATCCCAAT 
ACATCTGATA TGTCACTAGC AGAAATGGAT TACAAAGGAG CCTTTTCAAA AGGTCAAATC 
CTTTATGGCC GAGTACTTTG GAATCCAGAA CAAAATCTTA ATTCTGCTTA CAAACTCCAG 
CTGGAGAAAG TCTATCTTTG TACGGGCAAG GATGGTTATG TGCCTTTCTT TGATCCCACG 
GGGACAATCT ACAATGAAGG GCCCCAGTAT GGATGCATTC AGCCAAACAA ACACCTAAAA 
CACAGATTCC TGCTGTTGGA CCGCAATCAG CCAGAGGTAA CTGATAAGTA CTTCCATGAT 
GTGCCTTTTG AGGCTCACTT TGCCTCTGAG TTGCCTGATT TCCATGTGGT CAGTAACATG 
CCAGGTGTGG ATGGATTTAC TCTAAAAGTA GATGCACTCT ATAAGGTGGA AGCAGGACAC 
CAGTGGTATC TCCAGGTCAT CTACATCATT GGCCCTGACA CCATCTCAGG GCCCCGGGTC 
CAGCGCTCTC TCACAGCTCC ACTCAGACGC AACCGAAGGG ACCTGGTAGA GCCCGATGGC 
CAGCTGATCC TTGATGATTC CCTCATCTAT GACAATGAAG GAGACCAAGT CAAGAATGGC 
ACCAATATGA AGTCCCTGAA TCTGGAGATG CAAGAGTTGG CGGTAGCTGC GTCCCTGTCA 
CAGACTGGGG CGTCCATTGG CAGTGCCCTG GCTGCAATCA TGCTTCTACT TCTGGTGTTT 
TTGGTGGCTT GTTTTATCAA CAGGAAATGC CAGAAACAGA GGAAGAAGAA GCCCGCAGAG 
GACATTTTGG AAGAATATCC TCTGAATACC AAGGTAGAAG TGCCCAAGAG GCACCCGGAC 
CGGGTGGAGA AGAACGTGAA TAGACACTAC TGCACTGTGC GGAACGTCAA CATCCTGAGT 
GAGCCTGAGG CGGCTTACAC GTTCAAAGGT GCTAAAGTCA AAAGACTGAA TCTAGAAGTC 
AGAGTTCACA ACAATTTACA AGATGGAACA GAAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079350.5
CDS	441..12479
Translation	MGVLKVWLGLALALAEFAVLPHHSEGACVYQDSLLADATIWKPDSCQSCRCHGDIVICKPAVCRNPQCAFEKGEVLQIAANQCCPECVLRTPGSCHHEKKIHEHGTEWASSPCSVCSCNHGEVRCTPQPCPPLSCGHQELAFIPEGSCCPVCVGLGKPCSYEGHVFQDGEDWRLSRCAKCLCRNGVAQCFTAQCQPLFCNQDETVVRVPGKCCPQCSARSCSAAGQVYEHGEQWSENACTTCICDRGEVRCHKQACLPLRCGKGQSRARRHGQCCEECVSPAGSCSYDGVVRYQDEMWKGSACEFCMCDHGQVTCQTGECAKVECARDEELIHLDGKCCPECISRNGYCVYEETGEFMSSNASEVKRIPEGEKWEDGPCKVCECRGAQVTCYEPSCPPCPVGTLALEVKGQCCPDCTSVHCHPDCLTCSQSPDHCDLCQDPTKLLQNGWCVHSCGLGFYQAGSLCLACQPQCSTCTSGLECSSCQPPLLMRHGQCVPTCGDGFYQDRHSCAVCHESCAGCWGPTEKHCLACRDPLHVLRDGGCESSCGKGFYNRQGTCSACDQSCDSCGPSSPRCLTCTEKTVLHDGKCMSECPGGYYADATGRCKVCHNSCASCSGPTPSHCTACSPPKALRQGHCLPRCGEGFYSDHGVCKACHSSCLACMGPAPSHCTGCKKPEEGLQVEQLSDVGIPSGECLAQCRAHFYLESTGICEACHQSCFRCAGKSPHNCTDCGPSHVLLDGQCLSQCPDGYFHQEGSCTECHPTCRQCHGPLESDCISCYPHISLTNGNCRTSCREEQFLNLVGYCADCHHLCQHCAADLHNTGSICLRCQNAHYLLLGDHCVPDCPSGYYAERGACKKCHSSCRTCQGRGPFSCSSCDTNLVLSHTGTCSTTCFPGHYLDDNHVCQPCNTHCGSCDSQASCTSCRDPNKVLLFGECQYESCAPQYYLDFSTNTCKECDWSCSACSGPLKTDCLQCMDGYVLQDGACVEQCLSSFYQDSGLCKNCDSYCLQCQGPHECTRCKGPFLLLEAQCVQECGKGYFADHAKHKCTACPQGCLQCSHRDRCHLCDHGFFLKSGLCVYNCVPGFSVHTSNETCSGKIHTPSLHVNGSLILPIGSIKPLDFSLLNVQDQEGRVEDLLFHVVSTPTNGQLVLSRNGKEVQLDKAGRFSWKDVNEKKVRFVHSKEKLRKGYLFLKISDQQFFSEPQLINIQAFSTQAPYVLRNEVLHISRGERATITTQMLDIRDDDNPQDVVIEIIDPPLHGQLLQTLQSPATPIYQFQLDELSRGLLHYAHDGSDSTSDVAVLQANDGHSFHNILFQVKTVPQNDRGLQLVANSMVWVPEGGMLQITNRILQAEAPGASAEEIIYKITQDYPQFGEVVLLVNMPADSPADEGQHLPDGRTATPTSTFTQQDINEGIVWYRHSGAPAQSDSFRFEVSSASNAQTRLESHMFNIAILPQTPEAPKVSLEASLHMTAREDGLTVIQPHSLSFINSEKPSGKIVYNITLPLHPNQGIIEHRDHPHSPIRYFTQEDINQGKVMYRPPPAAPHLQELMAFSFAGLPESVKFHFTVSDGEHTSPEMVLTIHLLPSDQQLPVFQVTAPRLAVSPGGSTSVGLQVVVRDAETAPKELFFELRRPPQHGVLLKHTAEFRRPMATGDTFTYEDVEKNALQYIHDGSSTREDSMEISVTDGLTVTMLEVRVEVSLSEDRGPRLAAGSSLSITVASKSTAIITRSHLAYVDDSSPDPEIWIQLNYLPSYGTLLRISGSEVEELSEVSNFTMEDINNKKIRYSAVFETDGHLVTDSFYFSVSDMDHNHLDNQIFTIMITPAENPPPVIAFADLITVDEGGRAPLSFHHFFATDDDDNLQRDAIIKLSALPKYGCIENTGTGDRFGPETASDLEASFPIQDVLENYIYYFQSVHESIEPTHDIFSFYVSDGTSRSEIHSINITIERKNDEPPRMTLQPLRVQLSSGVVISNSSLSLQDLDTPDNELIFVLTKKPDHGHVLWRQTASEPLENGRVLVQGSTFTYQDILAGLVGYVPSVPGMVVDEFQFSLTDGLHVDTGRMKIYTELPASDTPHLAINQGLQLSAGSVARITEQHLKVTDIDSDDHQVMYIMKEDPGAGRLQMMKHGNLEQISIKGPIRSFTQADISQGHVEYSHGTGEPGGSFAFKFDVVDGEGNRLIDKSFSISILEDKSPPVITTNKGLVLDENSVKKITTLQLSATDQDSGPTELIYRITRQPQLGHLEHAASPGIQISSFTQADLTSRNVQYVHSSEAEKHSDAFSFTLSDGVSEVTQTFHITLHPVDDSLPVVQNLGMRVQEGMRKTITEFELKAVDADTEAESVTFTIVQPPRHGTIERTSNGQHFHLTSTFTMKDIYQNRVSYSHDGSNSLKDRFTFTVSDGTNPFFIIEEGGKEIMTAAPQPFRVDILPVDDGTPRIVTNLGLQWLEYMDGKATNLITKKELLTMDPDTEDAQLVYEITTGPKHGFVENKLQPGRAAATFTQEDVNLGLIRYVLHKEKIREMMDSFQFLVKDSKPNVVSDNVFHIQWSLISFKYTSYNVSEKAGSVSVTVQRTGNLNQYAIVLCRTEQGTASSSSRVSSQPGQQDYVEYAGQVQFDEREDTKSCTIVINDDDVFENVESFTVELSMPAYALLGEFTQAKVIINDTEDEPTLEFDKKIYWVNESAGFLFAPIERKGDASSIVSAICYTVPKSAMGSSLYALESGSDFKSRGMSAASRVIFGPGVTMSTCDVMLIDDSEYEEEEEFEIALADASDNARIGRVATAKVLISGPNDASTVSLGNTAFTVSEDAGTVKIPVIRHGTDLSTFASVWCATRPSDPASATPGVDYVPSSRKVEFGPGVIEQYCTLTILDDTQYPVIEGLETFVVFLSSAQGAELTKPFQAVIAINDTFQDVPSMQFAKDLLLVKEKEGVLHVPITRSGDLSYESSVRCYTQSHSAQVMEDFEERQNADSSRITFLKGDKVKNCTVYIHDDSMFEPEEQFRVYLGLPLGNHWSGARIGKNNMATITISNDEDAPTIEFEEAAYQVREPAGPDAIAILNIKVIRRGDQNRTSKVRCSTRDGSAQSGVDYYPKSRVLKFSPGVDHIFFKVEILSNEDREWHESFSLVLGPDDPVEAVLGDVTTATVTILDQEAAGSLILPAPPIVVTLADYDHVEEVTKEGVKKSPSPGYPLVCVTPCDPHFPRYAVMKERCSEAGINQTSVQFSWEVAAPTDGNGARSPFETITDNTPFTSVNHMVLDSIYFSRRFHVRCVAKAVDKVGHVGTPLRSNIVTIGTDSAICHTPVVAGTSRGFQAQSFIATLKYLDVKHKEHPNRIHISVQIPHQDGMLPLISTMPLHNLHFLLSESIYRHQHVCSNLVTTYDLRGISEAGFLDDVVYDSTALGPGYDRPFQFDPSVREPKTIQLYKHLNLKSCVWTFDAYYDMTELIDVCGGSVTADFQVRDSAQSFLTVHVPLYVSYIYVTAPRGWASLEHHTEMEFSFFYDTVLWRTGIQTDSVLSARLQIIRIYIREDGRLVIEFKTHAKFRGQFVMEHHTLPEVKSFVLTPDHLGGIEFDLQLLWSAQTFDSPHQLWRATSSYNRKDYSGEYTIYLIPCTVQPTQPWVDPGEKPLACTAHAPERFLIPIAFQQTNRPVPVVYSLNTEFQLCNNEKVFLMDPNTSDMSLAEMDYKGAFSKGQILYGRVLWNPEQNLNSAYKLQLEKVYLCTGKDGYVPFFDPTGTIYNEGPQYGCIQPNKHLKHRFLLLDRNQPEVTDKYFHDVPFEAHFASELPDFHVVSNMPGVDGFTLKVDALYKVEAGHQWYLQVIYIIGPDTISGPRVQRSLTAPLRRNRRDLVEPDGQLILDDSLIYDNEGDQVKNGTNMKSLNLEMQELAVAASLSQTGASIGSALAAIMLLLLVFLVACFINRKCQKQRKKKPAEDILEEYPLNTKVEVPKRHPDRVEKNVNRHYCTVRNVNILSEPEAAYTFKGAKVKRLNLEVRVHNNLQDGTEV

Target ORF information:

RefSeq Version	NM_025074.6
Organism	Homo sapiens(human)
Definition	Homo sapiens Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025074.6

ORF Insert Sequence:

ATGGGTGTCC TCAAAGTGTG GCTCGGGCTG GCCCTAGCGT TGGCGGAATT TGCAGTATTG 
CCTCATCATT CCGAAGGTGC TTGTGTCTAT CAGGATTCCT TGTTGGCGGA TGCCACAATT 
TGGAAGCCCG ATTCATGCCA GAGCTGCCGT TGCCATGGTG ATATTGTTAT CTGCAAACCT 
GCTGTTTGCA GAAACCCTCA ATGTGCCTTT GAGAAGGGAG AAGTGCTTCA AATAGCTGCC 
AACCAATGCT GTCCTGAGTG TGTTTTGAGG ACTCCAGGAT CTTGCCATCA TGAAAAGAAA 
ATCCATGAGC ATGGGACAGA ATGGGCCTCT TCTCCATGTA GTGTGTGCTC TTGCAATCAT 
GGGGAAGTCC GATGTACCCC CCAACCATGC CCACCGCTGT CATGTGGACA CCAGGAGCTG 
GCATTCATCC CTGAAGGAAG CTGCTGCCCA GTTTGTGTGG GCCTTGGGAA ACCCTGTTCC 
TATGAAGGCC ATGTGTTTCA GGATGGGGAG GACTGGCGGC TGAGCCGGTG TGCCAAATGT 
CTGTGTAGAA ATGGGGTTGC CCAGTGCTTC ACAGCTCAGT GTCAGCCTCT ATTTTGTAAC 
CAGGATGAGA CTGTAGTCCG AGTCCCTGGA AAATGTTGCC CGCAGTGCTC TGCAAGATCC 
TGCTCTGCAG CTGGCCAAGT ATACGAGCAT GGTGAGCAGT GGAGCGAAAA TGCCTGCACC 
ACGTGTATAT GTGACCGGGG TGAGGTCAGG TGTCACAAGC AGGCCTGCCT GCCCCTGAGA 
TGCGGAAAGG GTCAGAGCAG GGCTCGGCGT CATGGGCAAT GCTGTGAGGA ATGTGTGTCT 
CCTGCCGGGA GCTGCTCCTA TGATGGAGTT GTGCGGTACC AGGACGAAAT GTGGAAGGGC 
TCGGCCTGTG AGTTCTGCAT GTGTGATCAT GGCCAAGTGA CCTGCCAGAC TGGAGAGTGT 
GCCAAAGTGG AGTGTGCCCG GGATGAAGAA TTAATTCACT TAGATGGAAA GTGTTGTCCT 
GAATGCATTT CAAGGAATGG TTATTGTGTT TATGAAGAAA CTGGAGAATT TATGTCATCA 
AATGCTAGTG AAGTTAAACG TATTCCAGAG GGAGAGAAGT GGGAAGATGG CCCTTGCAAG 
GTGTGTGAGT GCCGAGGGGC TCAGGTAACT TGCTACGAGC CCTCTTGCCC ACCATGTCCA 
GTGGGCACAC TGGCCTTAGA GGTGAAGGGA CAGTGCTGTC CAGACTGCAC ATCAGTTCAT 
TGCCATCCAG ATTGTTTGAC ATGCTCTCAG TCTCCAGACC ACTGTGACCT CTGCCAAGAT 
CCTACCAAGT TACTGCAGAA TGGATGGTGT GTGCACAGCT GTGGACTGGG TTTTTACCAA 
GCTGGCAGTC TCTGTTTAGC CTGCCAGCCC CAGTGCTCCA CGTGTACCAG TGGGCTGGAG 
TGCTCATCCT GCCAGCCTCC CCTGCTGATG CGGCACGGGC AGTGTGTGCC TACCTGTGGG 
GACGGCTTCT ACCAAGATCG CCATTCCTGT GCAGTCTGCC ATGAGTCCTG TGCAGGTTGC 
TGGGGCCCAA CGGAGAAGCA CTGCTTGGCC TGCAGAGATC CCCTCCACGT GCTGAGAGAT 
GGCGGCTGTG AGAGCAGCTG TGGAAAAGGC TTCTACAACA GGCAGGGCAC CTGTAGCGCT 
TGTGACCAAT CCTGTGACAG TTGTGGCCCC AGTAGCCCCA GGTGTCTTAC CTGTACTGAG 
AAGACAGTGC TGCATGATGG GAAATGCATG TCTGAATGCC CTGGCGGGTA CTATGCTGAT 
GCCACTGGCA GGTGCAAAGT TTGTCATAAC TCATGTGCCA GCTGCTCTGG GCCCACACCC 
TCTCACTGTA CAGCCTGCAG CCCCCCCAAG GCTCTGCGTC AAGGCCACTG TCTGCCCCGC 
TGTGGAGAGG GTTTCTACTC TGACCATGGA GTCTGCAAAG CCTGTCACTC CTCCTGCCTG 
GCTTGTATGG GTCCCGCACC CTCTCACTGT ACTGGGTGTA AGAAGCCAGA GGAAGGACTG 
CAAGTGGAGC AGCTGTCTGA CGTGGGCATC CCCTCTGGCG AGTGTCTAGC CCAGTGTAGA 
GCCCATTTTT ACTTGGAGAG CACTGGCATA TGTGAAGCTT GCCACCAGTC CTGTTTCAGA 
TGTGCAGGGA AAAGCCCACA TAACTGCACA GACTGTGGGC CTTCCCATGT GCTGTTGGAT 
GGGCAGTGCC TCTCCCAGTG CCCAGATGGC TACTTTCACC AGGAAGGTAG TTGCACAGAG 
TGTCACCCAA CCTGCAGGCA GTGTCATGGG CCGTTGGAGT CTGACTGCAT CTCCTGTTAC 
CCTCACATCT CTCTTACCAA TGGTAACTGC AGGACCAGCT GCAGGGAAGA GCAGTTCCTC 
AACCTCGTGG GATACTGTGC TGACTGCCAT CACCTGTGCC AGCACTGTGC AGCTGATCTC 
CACAACACTG GGAGCATCTG CCTCAGGTGC CAGAATGCCC ACTACCTGCT GCTCGGGGAC 
CACTGTGTTC CTGACTGCCC TTCAGGATAC TATGCAGAGA GAGGAGCTTG TAAAAAATGC 
CACTCCTCCT GCAGAACCTG CCAGGGCAGA GGACCTTTCT CCTGCTCCTC ATGTGACACC 
AACCTCGTGC TGTCCCACAC TGGCACCTGC AGCACCACCT GCTTCCCTGG GCACTATCTT 
GATGACAATC ATGTTTGCCA GCCATGCAAC ACACACTGTG GAAGCTGTGA TTCACAGGCC 
AGCTGTACCT CCTGCCGAGA TCCAAACAAG GTTCTGCTCT TTGGGGAATG TCAATACGAG 
AGCTGCGCCC CACAGTACTA TCTTGACTTC TCCACCAACA CGTGCAAAGA GTGTGATTGG 
AGCTGCAGTG CATGCAGTGG GCCCCTGAAA ACAGACTGCC TGCAGTGCAT GGATGGCTAT 
GTTCTCCAGG ATGGGGCCTG CGTGGAGCAG TGCTTGTCAT CATTTTACCA GGACTCGGGC 
CTCTGCAAGA ACTGTGACAG CTACTGTCTC CAGTGCCAAG GTCCCCATGA GTGTACCCGC 
TGCAAAGGGC CATTTCTCCT CTTGGAAGCC CAGTGTGTCC AGGAATGTGG GAAGGGGTAC 
TTTGCAGATC ATGCAAAGCA CAAATGCACA GCCTGCCCTC AGGGGTGCTT GCAGTGCAGC 
CACAGGGACC GTTGTCACCT CTGTGACCAT GGGTTCTTTC TGAAGAGTGG CCTCTGTGTT 
TACAACTGTG TTCCTGGCTT TTCTGTCCAC ACCTCTAATG AAACATGTTC TGGCAAAATA 
CACACCCCTA GTCTTCATGT GAATGGTTCC CTGATCCTCC CAATTGGTTC AATAAAGCCA 
CTGGATTTTT CCCTCCTGAA TGTCCAAGAC CAGGAGGGTA GGGTCGAAGA TCTCCTATTT 
CATGTTGTGA GCACTCCCAC CAATGGTCAG CTAGTGCTCT CAAGAAATGG AAAAGAGGTT 
CAGCTGGACA AGGCTGGCCG TTTTAGCTGG AAAGATGTGA ACGAGAAGAA AGTGCGTTTT 
GTGCACAGCA AAGAAAAACT CAGGAAAGGT TACCTTTTCC TGAAAATTAG TGACCAGCAG 
TTCTTCTCTG AGCCACAGCT GATCAACATA CAAGCATTTT CAACACAGGC CCCCTATGTG 
CTGAGAAATG AAGTTCTCCA CATTAGCAGA GGAGAGAGGG CAACCATCAC CACCCAGATG 
CTTGACATCC GAGATGATGA CAACCCACAG GATGTGGTCA TTGAAATAAT CGATCCTCCA 
CTTCATGGCC AATTGCTTCA GACACTTCAG TCCCCGGCAA CCCCTATCTA TCAATTCCAG 
CTGGATGAAC TCTCTAGAGG CCTTCTCCAC TATGCTCATG ATGGTTCAGA CAGCACATCC 
GATGTTGCAG TCTTGCAGGC CAATGATGGA CACTCCTTCC ATAATATACT GTTCCAAGTG 
AAGACCGTGC CTCAGAATGA CAGGGGTCTT CAGCTTGTGG CTAATTCGAT GGTGTGGGTT 
CCAGAAGGGG GGATGCTGCA GATCACCAAC AGAATCTTAC AGGCCGAGGC TCCTGGTGCC 
AGTGCTGAAG AAATCATCTA CAAGATTACA CAAGACTACC CCCAGTTTGG GGAGGTGGTC 
CTTCTAGTGA ATATGCCTGC AGACAGCCCT GCAGATGAAG GGCAGCACCT GCCTGATGGG 
AGGACAGCTA CCCCCACCAG CACCTTCACC CAGCAGGACA TCAATGAAGG CATCGTATGG 
TACAGGCACT CAGGAGCCCC AGCCCAGAGC GACTCCTTCC GCTTCGAGGT GTCCAGTGCC 
TCCAATGCCC AGACCCGCCT GGAGAGCCAC ATGTTCAACA TCGCGATCTT ACCACAGACA 
CCTGAAGCAC CTAAAGTGTC TCTGGAAGCA TCTCTCCATA TGACTGCTAG AGAAGATGGC 
CTGACTGTTA TTCAGCCTCA TTCCCTCTCC TTCATAAACT CTGAGAAGCC AAGTGGAAAG 
ATTGTCTACA ACATCACTCT ACCTCTGCAT CCAAATCAAG GTATCATCGA GCACCGGGAC 
CACCCTCACT CTCCTATCCG GTATTTCACG CAAGAGGATA TTAACCAGGG CAAAGTCATG 
TACCGCCCTC CCCCGGCAGC ACCCCACCTC CAGGAGCTCA TGGCCTTCTC GTTCGCTGGT 
CTCCCAGAAT CAGTGAAATT CCACTTCACA GTTTCAGATG GAGAACACAC AAGTCCGGAG 
ATGGTCCTCA CCATTCACTT ACTTCCCAGT GATCAGCAAC TGCCAGTGTT CCAGGTCACA 
GCTCCACGGC TGGCGGTCAG CCCAGGAGGC AGCACTTCTG TAGGACTTCA GGTGGTAGTA 
AGAGATGCTG AGACAGCGCC CAAAGAACTC TTCTTTGAGC TTCGGAGACC TCCACAGCAT 
GGTGTGCTTC TTAAGCATAC AGCTGAGTTC CGAAGGCCGA TGGCCACAGG TGACACTTTC 
ACCTATGAGG ATGTTGAGAA AAATGCTCTA CAGTATATAC ATGATGGTTC CTCTACCCGG 
GAAGACAGCA TGGAGATCTC AGTCACAGAT GGCCTCACAG TGACAATGCT GGAGGTGAGA 
GTAGAGGTGT CCCTGTCAGA AGACCGAGGG CCTCGACTGG CTGCTGGCTC CTCTCTGAGC 
ATTACTGTTG CCAGTAAAAG CACAGCCATA ATCACTAGGT CACACCTTGC TTACGTGGAT 
GATTCTTCCC CCGACCCAGA GATCTGGATT CAGTTAAATT ATCTGCCCTC ATATGGTACT 
CTCTTAAGAA TCTCAGGATC TGAGGTGGAA GAGCTCTCAG AAGTTTCCAA TTTCACAATG 
GAAGACATCA ATAACAAGAA AATCAGATAC TCAGCTGTGT TTGAAACTGA TGGTCATCTG 
GTTACTGATA GCTTCTATTT CTCTGTCTCT GACATGGACC ACAACCATCT GGATAATCAG 
ATATTTACCA TCATGATCAC TCCTGCTGAA AATCCACCTC CAGTCATTGC TTTTGCTGAC 
CTTATCACGG TTGATGAGGG AGGGAGAGCA CCACTCTCAT TTCACCATTT TTTTGCTACT 
GATGATGATG ACAACCTCCA GAGAGATGCC ATCATTAAAC TAAGTGCTCT GCCCAAATAT 
GGCTGCATTG AGAACACAGG AACAGGTGAT CGTTTTGGCC CTGAAACTGC CAGTGACCTA 
GAGGCATCAT TTCCTATTCA AGACGTCCTG GAAAACTACA TTTACTACTT TCAGAGTGTT 
CATGAAAGCA TTGAGCCAAC CCATGATATT TTTAGTTTTT ATGTGAGTGA TGGAACCAGT 
CGTTCAGAAA TTCACAGCAT CAATATCACC ATTGAGAGGA AGAACGATGA GCCTCCCAGG 
ATGACCTTGC AGCCCCTCAG AGTGCAGCTG AGCTCGGGAG TGGTGATAAG CAATTCTTCT 
TTGAGCCTGC AAGACCTGGA CACCCCAGAT AATGAGCTCA TTTTTGTATT GACAAAAAAG 
CCTGACCACG GTCATGTACT CTGGAGGCAA ACTGCTTCTG AGCCTCTGGA GAATGGGAGA 
GTTTTAGTCC AGGGCTCAAC CTTCACCTAC CAGGATATCC TAGCTGGGCT GGTTGGGTAT 
GTGCCTAGTG TCCCTGGCAT GGTCGTGGAT GAGTTCCAGT TCTCCCTCAC TGATGGCCTC 
CACGTGGACA CAGGGAGGAT GAAGATCTAC ACAGAACTGC CTGCAAGTGA CACACCTCAC 
TTGGCTATAA ACCAAGGCCT ACAGCTCTCA GCAGGGTCTG TAGCACGCAT CACAGAACAG 
CACTTGAAAG TGACAGATAT TGACTCAGAT GACCATCAGG TTATGTACAT CATGAAGGAA 
GATCCTGGTG CAGGGCGCCT GCAGATGATG AAGCATGGCA ACCTGGAGCA AATTTCTATT 
AAAGGCCCCA TCCGAAGTTT CACCCAGGCA GACATTAGCC AAGGCCACGT AGAATATAGT 
CATGGAACAG GAGAACCTGG AGGGAGCTTT GCTTTTAAAT TTGATGTGGT TGATGGAGAA 
GGCAACAGAT TGATTGACAA GTCATTTTCC ATCAGCATTC TAGAAGACAA ATCCCCACCA 
GTCATCACCA CCAATAAAGG ACTGGTCTTG GATGAAAACT CAGTGAAGAA AATCACCACC 
CTGCAGCTGT CTGCCACTGA CCAGGACAGT GGGCCTACAG AATTGATCTA CAGAATCACC 
AGACAGCCCC AGCTGGGCCA CTTGGAACAT GCAGCATCAC CAGGTATCCA GATTAGTTCC 
TTTACTCAAG CTGATCTGAC TTCACGAAAT GTTCAGTATG TCCATTCTAG TGAGGCTGAG 
AAACATTCAG ATGCCTTCAG CTTTACACTG TCTGATGGAG TCAGTGAGGT GACTCAGACT 
TTCCATATCA CTCTTCACCC TGTCGATGAT TCGCTGCCCG TCGTACAGAA CTTAGGAATG 
CGGGTGCAGG AGGGCATGAG GAAGACCATC ACAGAGTTTG AGCTTAAGGC GGTGGATGCT 
GACACAGAGG CCGAGTCTGT CACATTCACC ATCGTGCAGC CTCCACGCCA TGGCACCATC 
GAGCGAACCA GCAATGGGCA GCATTTCCAC CTCACCTCCA CCTTCACCAT GAAAGATATC 
TACCAGAACC GGGTCAGCTA CAGCCATGAC GGCAGTAACT CCCTCAAGGA CCGGTTCACC 
TTCACTGTTT CTGATGGGAC AAACCCCTTC TTTATCATTG AGGAAGGGGG AAAAGAGATT 
ATGACAGCAG CACCTCAGCC GTTCCGAGTA GACATCCTCC CGGTAGATGA TGGCACGCCT 
AGAATTGTCA CCAACCTGGG ACTCCAGTGG CTGGAATACA TGGATGGCAA GGCAACCAAC 
CTGATCACCA AGAAGGAACT GCTGACCATG GACCCAGACA CCGAGGACGC GCAGCTTGTC 
TATGAGATAA CGACGGGCCC TAAGCATGGC TTTGTGGAGA ACAAGCTGCA GCCTGGCAGA 
GCTGCTGCCA CTTTCACCCA GGAGGATGTG AACTTGGGGT TGATTCGTTA TGTGTTGCAC 
AAGGAGAAGA TCCGTGAGAT GATGGATAGT TTTCAGTTTC TGGTGAAAGA CAGTAAACCC 
AATGTGGTCA GCGACAATGT CTTCCATATC CAGTGGTCAC TCATCAGCTT TAAATATACC 
AGCTACAATG TCAGTGAGAA GGCAGGGTCT GTCAGTGTCA CGGTGCAGAG GACTGGGAAC 
CTGAACCAAT ATGCCATCGT CCTGTGTCGC ACCGAGCAAG GCACCGCCAG CTCCAGCTCC 
AGGGTCAGCT CCCAACCTGG GCAACAGGAC TATGTAGAGT ATGCTGGCCA GGTCCAGTTT 
GATGAGCGAG AGGACACCAA GTCCTGCACC ATTGTCATCA ACGATGATGA CGTGTTTGAA 
AATGTTGAGA GTTTCACTGT GGAGCTCAGC ATGCCAGCTT ATGCCCTGTT AGGGGAATTC 
ACCCAGGCGA AGGTCATTAT CAACGATACC GAGGATGAAC CCACATTAGA GTTTGACAAG 
AAGATCTACT GGGTTAACGA GAGCGCTGGT TTTCTGTTTG CACCTATTGA AAGAAAAGGA 
GATGCAAGCA GCATTGTATC TGCAATTTGC TACACAGTCC CTAAGTCAGC TATGGGAAGT 
AGCCTCTATG CTCTAGAATC AGGCTCTGAT TTTAAATCTA GAGGGATGTC TGCCGCGAGT 
CGTGTGATAT TCGGGCCTGG TGTGACCATG TCCACCTGTG ATGTCATGCT TATTGATGAC 
AGCGAGTATG AAGAGGAAGA AGAGTTTGAG ATTGCCTTGG CAGATGCCTC TGACAATGCC 
CGCATTGGAA GGGTGGCGAC AGCCAAGGTG CTCATTAGTG GTCCCAACGA TGCCTCGACT 
GTGTCCCTGG GCAACACGGC TTTCACTGTC AGTGAGGACG CAGGCACAGT AAAGATTCCA 
GTTATCCGCC ATGGTACTGA CCTCTCTACT TTCGCATCTG TCTGGTGTGC AACGCGGCCC 
TCAGACCCAG CTTCTGCCAC ACCAGGAGTT GACTACGTTC CCAGCTCTCG GAAGGTGGAA 
TTTGGGCCTG GTGTCATTGA ACAGTATTGC ACCTTGACTA TCTTGGATGA CACTCAGTAT 
CCGGTAATTG AAGGACTGGA GACATTTGTG GTTTTCCTCA GCTCAGCACA AGGAGCCGAA 
CTGACCAAAC CCTTCCAGGC AGTCATTGCA ATTAATGACA CATTCCAAGA TGTGCCCAGC 
ATGCAGTTTG CCAAGGATTT GCTCCTAGTG AAGGAGAAGG AGGGTGTCCT GCATGTCCCT 
ATCACTCGGA GCGGAGACCT GAGCTATGAG TCATCAGTGA GGTGCTATAC TCAGAGCCAT 
TCCGCTCAGG TCATGGAGGA CTTTGAGGAG AGACAAAATG CAGACTCTTC ACGGATTACA 
TTTCTCAAAG GGGACAAAGT GAAGAACTGT ACGGTCTATA TCCACGATGA CTCCATGTTT 
GAGCCAGAGG AACAGTTCAG GGTCTACCTC GGCCTTCCTC TTGGAAACCA CTGGAGTGGA 
GCTAGAATTG GAAAGAATAA CATGGCCACC ATCACCATAT CCAATGATGA AGATGCCCCC 
ACCATTGAGT TTGAAGAAGC TGCATACCAA GTCCGGGAAC CCGCAGGCCC AGATGCCATT 
GCGATTCTGA ACATCAAGGT GATCCGCAGA GGGGATCAGA ACAGGACCTC CAAGGTTCGC 
TGCAGCACGC GGGATGGCTC TGCCCAGTCT GGTGTGGATT ATTACCCAAA GAGCCGAGTC 
TTGAAGTTCA GTCCCGGTGT GGATCATATC TTTTTTAAAG TTGAGATCCT GTCCAATGAA 
GACCGGGAAT GGCATGAATC TTTCTCACTA GTCCTTGGCC CAGATGACCC AGTGGAAGCA 
GTTCTTGGGG ATGTGACTAC TGCCACGGTG ACAATTCTAG ACCAGGAGGC AGCAGGGAGC 
CTCATATTGC CAGCACCACC CATTGTGGTC ACACTTGCTG ACTATGACCA TGTGGAAGAA 
GTTACCAAGG AAGGAGTCAA GAAATCCCCC TCCCCAGGCT ACCCACTGGT CTGTGTCACC 
CCCTGCGACC CTCATTTCCC CAGATACGCT GTCATGAAGG AGCGCTGCAG TGAGGCCGGC 
ATCAACCAGA CATCTGTGCA GTTCAGCTGG GAAGTGGCTG CCCCCACTGA TGGCAATGGG 
GCCCGGTCTC CCTTTGAAAC CATCACTGAC AACACACCAT TCACCAGTGT CAACCACATG 
GTCCTGGACA GCATTTACTT CAGCCGGAGG TTCCATGTGC GTTGTGTGGC CAAGGCTGTG 
GACAAGGTGG GCCATGTGGG GACCCCCTTA AGGAGCAACA TTGTTACCAT TGGAACAGAC 
AGTGCTATCT GCCACACACC AGTGGTGGCT GGGACATCCA GAGGCTTCCA GGCTCAGTCC 
TTCATCGCAA CCTTGAAATA CCTGGATGTC AAACATAAGG AGCATCCGAA CAGAATCCAC 
ATTTCGGTGC AGATCCCACA CCAGGATGGA ATGCTGCCCC TTATCTCCAC CATGCCGTTG 
CACAACTTAC ATTTTCTACT GTCTGAGTCC ATCTACAGAC ACCAGCACGT CTGCTCCAAT 
TTAGTTACCA CCTATGACCT GAGAGGCATC TCAGAGGCAG GGTTCCTGGA TGATGTGGTC 
TATGATAGCA CTGCCCTGGG GCCTGGCTAC GATCGCCCCT TCCAGTTTGA CCCCAGCGTG 
CGAGAGCCGA AGACCATCCA GCTCTACAAA CACCTGAACC TGAAGAGCTG CGTGTGGACC 
TTTGATGCTT ATTATGACAT GACTGAGCTG ATTGACGTCT GTGGGGGCTC TGTAACCGCT 
GACTTCCAGG TGAGGGACTC TGCCCAGTCC TTCTTGACAG TGCACGTGCC TCTATATGTG 
TCCTACATCT ATGTGACAGC CCCCAGGGGC TGGGCCTCCT TGGAGCACCA CACCGAGATG 
GAGTTTTCTT TCTTCTATGA CACTGTTCTC TGGAGAACAG GAATCCAGAC AGACAGCGTG 
CTCTCTGCAA GGCTTCAGAT AATAAGAATC TACATTCGAG AGGATGGCCG TCTTGTCATT 
GAATTCAAGA CCCATGCCAA ATTCAGAGGA CAGTTTGTGA TGGAGCATCA CACTCTCCCA 
GAAGTGAAAT CTTTCGTATT GACTCCAGAC CACCTAGGAG GAATTGAATT TGACTTGCAG 
CTATTATGGA GCGCTCAGAC TTTTGATTCT CCACATCAAC TCTGGAGAGC CACAAGCTCT 
TATAACAGGA AGGACTACTC AGGAGAGTAC ACCATCTACC TGATCCCTTG CACAGTGCAG 
CCCACACAGC CATGGGTTGA CCCAGGAGAG AAGCCTTTGG CCTGCACTGC ACATGCCCCA 
GAAAGATTCC TGATACCCAT TGCATTCCAG CAGACCAACC GCCCTGTGCC AGTTGTGTAT 
TCACTTAACA CTGAATTTCA GCTCTGCAAT AATGAGAAGG TGTTCCTAAT GGATCCCAAT 
ACATCTGATA TGTCACTAGC AGAAATGGAT TACAAAGGAG CCTTTTCAAA AGGTCAAATC 
CTTTATGGCC GAGTACTTTG GAATCCAGAA CAAAATCTTA ATTCTGCTTA CAAACTCCAG 
CTGGAGAAAG TCTATCTTTG TACGGGCAAG GATGGTTATG TGCCTTTCTT TGATCCCACG 
GGGACAATCT ACAATGAAGG GCCCCAGTAT GGATGCATTC AGCCAAACAA ACACCTAAAA 
CACAGATTCC TGCTGTTGGA CCGCAATCAG CCAGAGGTAA CTGATAAGTA CTTCCATGAT 
GTGCCTTTTG AGGCTCACTT TGCCTCTGAG TTGCCTGATT TCCATGTGGT CAGTAACATG 
CCAGGTGTGG ATGGATTTAC TCTAAAAGTA GATGCACTCT ATAAGGTGGA AGCAGGACAC 
CAGTGGTATC TCCAGGTCAT CTACATCATT GGCCCTGACA CCATCTCAGG GCCCCGGGTC 
CAGCGCTCTC TCACAGCTCC ACTCAGACGC AACCGAAGGG ACCTGGTAGA GCCCGATGGC 
CAGCTGATCC TTGATGATTC CCTCATCTAT GACAATGAAG GAGACCAAGT CAAGAATGGC 
ACCAATATGA AGTCCCTGAA TCTGGAGATG CAAGAGTTGG CGGTAGCTGC GTCCCTGTCA 
CAGACTGGGG CGTCCATTGG CAGTGCCCTG GCTGCAATCA TGCTTCTACT TCTGGTGTTT 
TTGGTGGCTT GTTTTATCAA CAGGAAATGC CAGAAACAGA GGAAGAAGAA GCCCGCAGAG 
GACATTTTGG AAGAATATCC TCTGAATACC AAGGTAGAAG TGCCCAAGAG GCACCCGGAC 
CGGGTGGAGA AGAACGTGAA TAGACACTAC TGCACTGTGC GGAACGTCAA CATCCTGAGT 
GAGCCTGAGG CGGCTTACAC GTTCAAAGGT GCTAAAGTCA AAAGACTGAA TCTAGAAGTC 
AGAGTTCACA ACAATTTACA AGATGGAACA GAAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FRAS1 ( XM_006714316.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714379.1 Homo sapiens Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant X1, mRNA.

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CloneID	OHu39089
Clone ID Related Accession (Same CDS sequence)	XM_006714316.3 , XM_006714316.2
Accession Version	XM_006714316.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	extracellular matrix protein FRAS1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006714316.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGTCC TCAAAGTGTG GCTCGGGCTG GCCCTAGCGT TGGCGGAATT TGCAGTATTG 
CCTCATCATT CCGAAGGTGC TTGTGTCTAT CAGGATTCCT TGTTGGCGGA TGCCACAATT 
TGGAAGCCCG ATTCATGCCA GAGCTGCCGT TGCCATGGTG ATATTGTTAT CTGCAAACCT 
GCTGTTTGCA GAAACCCTCA ATGTGCCTTT GAGAAGGGAG AAGTGCTTCA AATAGCTGCC 
AACCAATGCT GTCCTGAGTG TGTTTTGAGG ACTCCAGGAT CTTGCCATCA TGAAAAGAAA 
ATCCATGAGC ATGGGACAGA ATGGGCCTCT TCTCCATGTA GTGTGTGCTC TTGCAATCAT 
GGGGAAGTCC GATGTACCCC CCAACCATGC CCACCGCTGT CATGTGGACA CCAGGAGCTG 
GCATTCATCC CTGAAGGAAG CTGCTGCCCA GTTTGTGTGG GCCTTGGGAA ACCCTGTTCC 
TATGAAGGCC ATGTGTTTCA GGATGGGGAG GACTGGCGGC TGAGCCGGTG TGCCAAATGT 
CTGTGTAGAA ATGGGGTTGC CCAGTGCTTC ACAGCTCAGT GTCAGCCTCT ATTTTGTAAC 
CAGGATGAGA CTGTAGTCCG AGTCCCTGGA AAATGTTGCC CGCAGTGCTC TGCAAGATCC 
TGCTCTGCAG CTGGCCAAGT ATACGAGCAT GGTGAGCAGT GGAGCGAAAA TGCCTGCACC 
ACGTGTATAT GTGACCGGGG TGAGGTCAGG TGTCACAAGC AGGCCTGCCT GCCCCTGAGA 
TGCGGAAAGG GTCAGAGCAG GGCTCGGCGT CATGGGCAAT GCTGTGAGGA ATGTGTGTCT 
CCTGCCGGGA GCTGCTCCTA TGATGGAGTT GTGCGGTACC AGGACGAAAT GTGGAAGGGC 
TCGGCCTGTG AGTTCTGCAT GTGTGATCAT GGCCAAGTGA CCTGCCAGAC TGGAGAGTGT 
GCCAAAGTGG AGTGTGCCCG GGATGAAGAA TTAATTCACT TAGATGGAAA GTGTTGTCCT 
GAATGCATTT CAAGGAATGG TTATTGTGTT TATGAAGAAA CTGGAGAATT TATGTCATCA 
AATGCTAGTG AAGTTAAACG TATTCCAGAG GGAGAGAAGT GGGAAGATGG CCCTTGCAAG 
GTGTGTGAGT GCCGAGGGGC TCAGGTAACT TGCTACGAGC CCTCTTGCCC ACCATGTCCA 
GTGGGCACAC TGGCCTTAGA GGTGAAGGGA CAGTGCTGTC CAGACTGCAC ATCAGTTCAT 
TGCCATCCAG ATTGTTTGAC ATGCTCTCAG TCTCCAGACC ACTGTGACCT CTGCCAAGAT 
CCTACCAAGT TACTGCAGAA TGGATGGTGT GTGCACAGCT GTGGACTGGG TTTTTACCAA 
GCTGGCAGTC TCTGTTTAGC CTGCCAGCCC CAGTGCTCCA CGTGTACCAG TGGGCTGGAG 
TGCTCATCCT GCCAGCCTCC CCTGCTGATG CGGCACGGGC AGTGTGTGCC TACCTGTGGG 
GACGGCTTCT ACCAAGATCG CCATTCCTGT GCAGTCTGCC ATGAGTCCTG TGCAGGTTGC 
TGGGGCCCAA CGGAGAAGCA CTGCTTGGCC TGCAGAGATC CCCTCCACGT GCTGAGAGAT 
GGCGGCTGTG AGAGCAGCTG TGGAAAAGGC TTCTACAACA GGCAGGGCAC CTGTAGCGCT 
TGTGACCAAT CCTGTGACAG TTGTGGCCCC AGTAGCCCCA GGTGTCTTAC CTGTACTGAG 
AAGACAGTGC TGCATGATGG GAAATGCATG TCTGAATGCC CTGGCGGGTA CTATGCTGAT 
GCCACTGGCA GGTGCAAAGT TTGTCATAAC TCATGTGCCA GCTGCTCTGG GCCCACACCC 
TCTCACTGTA CAGCCTGCAG CCCCCCCAAG GCTCTGCGTC AAGGCCACTG TCTGCCCCGC 
TGTGGAGAGG GTTTCTACTC TGACCATGGA GTCTGCAAAG CCTGTCACTC CTCCTGCCTG 
GCTTGTATGG GTCCCGCACC CTCTCACTGT ACTGGGTGTA AGAAGCCAGA GGAAGGACTG 
CAAGTGGAGC AGCTGTCTGA CGTGGGCATC CCCTCTGGCG AGTGTCTAGC CCAGTGTAGA 
GCCCATTTTT ACTTGGAGAG CACTGGCATA TGTGAAGCTT GCCACCAGTC CTGTTTCAGA 
TGTGCAGGGA AAAGCCCACA TAACTGCACA GACTGTGGGC CTTCCCATGT GCTGTTGGAT 
GGGCAGTGCC TCTCCCAGTG CCCAGATGGC TACTTTCACC AGGAAGGTAG TTGCACAGAG 
TGTCACCCAA CCTGCAGGCA GTGTCATGGG CCGTTGGAGT CTGACTGCAT CTCCTGTTAC 
CCTCACATCT CTCTTACCAA TGGTAACTGC AGGACCAGCT GCAGGGAAGA GCAGTTCCTC 
AACCTCGTGG GATACTGTGC TGACTGCCAT CACCTGTGCC AGCACTGTGC AGCTGATCTC 
CACAACACTG GGAGCATCTG CCTCAGGTGC CAGAATGCCC ACTACCTGCT GCTCGGGGAC 
CACTGTGTTC CTGACTGCCC TTCAGGATAC TATGCAGAGA GAGGAGCTTG TAAAAAATGC 
CACTCCTCCT GCAGAACCTG CCAGGGCAGA GGACCTTTCT CCTGCTCCTC ATGTGACACC 
AACCTCGTGC TGTCCCACAC TGGCACCTGC AGCACCACCT GCTTCCCTGG GCACTATCTT 
GATGACAATC ATGTTTGCCA GCCATGCAAC ACACACTGTG GAAGCTGTGA TTCACAGGCC 
AGCTGTACCT CCTGCCGAGA TCCAAACAAG GTTCTGCTCT TTGGGGAATG TCAATACGAG 
AGCTGCGCCC CACAGTACTA TCTTGACTTC TCCACCAACA CGTGCAAAGA GTGTGATTGG 
AGCTGCAGTG CATGCAGTGG GCCCCTGAAA ACAGACTGCC TGCAGTGCAT GGATGGCTAT 
GTTCTCCAGG ATGGGGCCTG CGTGGAGCAG TGCTTGTCAT CATTTTACCA GGACTCGGGC 
CTCTGCAAGA ACTGTGACAG CTACTGTCTC CAGTGCCAAG GTCCCCATGA GTGTACCCGC 
TGCAAAGGGC CATTTCTCCT CTTGGAAGCC CAGTGTGTCC AGGAATGTGG GAAGGGGTAC 
TTTGCAGATC ATGCAAAGCA CAAATGCACA GCCTGCCCTC AGGGGTGCTT GCAGTGCAGC 
CACAGGGACC GTTGTCACCT CTGTGACCAT GGGTTCTTTC TGAAGAGTGG CCTCTGTGTT 
TACAACTGTG TTCCTGGCTT TTCTGTCCAC ACCTCTAATG AAACATGTTC TGGCAAAATA 
CACACCCCTA GTCTTCATGT GAATGGTTCC CTGATCCTCC CAATTGGTTC AATAAAGCCA 
CTGGATTTTT CCCTCCTGAA TGTCCAAGAC CAGGAGGGTA GGGTCGAAGA TCTCCTATTT 
CATGTTGTGA GCACTCCCAC CAATGGTCAG CTAGTGCTCT CAAGAAATGG AAAAGAGGTT 
CAGCTGGACA AGGCTGGCCG TTTTAGCTGG AAAGATGTGA ACGAGAAGAA AGTGCGTTTT 
GTGCACAGCA AAGAAAAACT CAGGAAAGGT TACCTTTTCC TGAAAATTAG TGACCAGCAG 
TTCTTCTCTG AGCCACAGCT GATCAACATA CAAGCATTTT CAACACAGGC CCCCTATGTG 
CTGAGAAATG AAGTTCTCCA CATTAGCAGA GGAGAGAGGG CAACCATCAC CACCCAGATG 
CTTGACATCC GAGATGATGA CAACCCACAG GATGTGGTCA TTGAAATAAT CGATCCTCCA 
CTTCATGGCC AATTGCTTCA GACACTTCAG TCCCCGGCAA CCCCTATCTA TCAATTCCAG 
CTGGATGAAC TCTCTAGAGG CCTTCTCCAC TATGCTCATG ATGGTTCAGA CAGCACATCC 
GATGTTGCAG TCTTGCAGGC CAATGATGGA CACTCCTTCC ATAATATACT GTTCCAAGTG 
AAGACCGTGC CTCAGAATGA CAGGGGTCTT CAGCTTGTGG CTAATTCGAT GGTGTGGGTT 
CCAGAAGGGG GGATGCTGCA GATCACCAAC AGAATCTTAC AGGCCGAGGC TCCTGGTGCC 
AGTGCTGAAG AAATCATCTA CAAGATTACA CAAGACTACC CCCAGTTTGG GGAGGTGGTC 
CTTCTAGTGA ATATGCCTGC AGACAGCCCT GCAGATGAAG GGCAGCACCT GCCTGATGGG 
AGGACAGCTA CCCCCACCAG CACCTTCACC CAGCAGGACA TCAATGAAGG CATCGTATGG 
TACAGGCACT CAGGAGCCCC AGCCCAGAGC GACTCCTTCC GCTTCGAGGT GTCCAGTGCC 
TCCAATGCCC AGACCCGCCT GGAGAGCCAC ATGTTCAACA TCGCGATCTT ACCACAGACA 
CCTGAAGCAC CTAAAGTGTC TCTGGAAGCA TCTCTCCATA TGACTGCTAG AGAAGATGGC 
CTGACTGTTA TTCAGCCTCA TTCCCTCTCC TTCATAAACT CTGAGAAGCC AAGTGGAAAG 
ATTGTCTACA ACATCACTCT ACCTCTGCAT CCAAATCAAG GTATCATCGA GCACCGGGAC 
CACCCTCACT CTCCTATCCG GTATTTCACG CAAGAGGATA TTAACCAGGG CAAAGTCATG 
TACCGCCCTC CCCCGGCAGC ACCCCACCTC CAGGAGCTCA TGGCCTTCTC GTTCGCTGGT 
CTCCCAGAAT CAGTGAAATT CCACTTCACA GTTTCAGATG GAGAACACAC AAGTCCGGAG 
ATGGTCCTCA CCATTCACTT ACTTCCCAGT GATCAGCAAC TGCCAGTGTT CCAGGTCACA 
GCTCCACGGC TGGCGGTCAG CCCAGGAGGC AGCACTTCTG TAGGACTTCA GGTGGTAGTA 
AGAGATGCTG AGACAGCGCC CAAAGAACTC TTCTTTGAGC TTCGGAGACC TCCACAGCAT 
GGTGTGCTTC TTAAGCATAC AGCTGAGTTC CGAAGGCCGA TGGCCACAGG TGACACTTTC 
ACCTATGAGG ATGTTGAGAA AAATGCTCTA CAGTATATAC ATGATGGTTC CTCTACCCGG 
GAAGACAGCA TGGAGATCTC AGTCACAGAT GGCCTCACAG TGACAATGCT GGAGGTGAGA 
GTAGAGGTGT CCCTGTCAGA AGACCGAGGG CCTCGACTGG CTGCTGGCTC CTCTCTGAGC 
ATTACTGTTG CCAGTAAAAG CACAGCCATA ATCACTAGGT CACACCTTGC TTACGTGGAT 
GATTCTTCCC CCGACCCAGA GATCTGGATT CAGTTAAATT ATCTGCCCTC ATATGGTACT 
CTCTTAAGAA TCTCAGGATC TGAGGTGGAA GAGCTCTCAG AAGTTTCCAA TTTCACAATG 
GAAGACATCA ATAACAAGAA AATCAGATAC TCAGCTGTGT TTGAAACTGA TGGTCATCTG 
GTTACTGATA GCTTCTATTT CTCTGTCTCT GACATGGACC ACAACCATCT GGATAATCAG 
ATATTTACCA TCATGATCAC TCCTGCTGAA AATCCACCTC CAGTCATTGC TTTTGCTGAC 
CTTATCACGG TTGATGAGGG AGGGAGAGCA CCACTCTCAT TTCACCATTT TTTTGCTACT 
GATGATGATG ACAACCTCCA GAGAGATGCC ATCATTAAAC TAAGTGCTCT GCCCAAATAT 
GGCTGCATTG AGAACACAGG AACAGGTGAT CGTTTTGGCC CTGAAACTGC CAGTGACCTA 
GAGGCATCAT TTCCTATTCA AGACGTCCTG GAAAACTACA TTTACTACTT TCAGAGTGTT 
CATGAAAGCA TTGAGCCAAC CCATGATATT TTTAGTTTTT ATGTGAGTGA TGGAACCAGT 
CGTTCAGAAA TTCACAGCAT CAATATCACC ATTGAGAGGA AGAACGATGA GCCTCCCAGG 
ATGACCTTGC AGCCCCTCAG AGTGCAGCTG AGCTCGGGAG TGGTGATAAG CAATTCTTCT 
TTGAGCCTGC AAGACCTGGA CACCCCAGAT AATGAGCTCA TTTTTGTATT GACAAAAAAG 
CCTGACCACG GTCATGTACT CTGGAGGCAA ACTGCTTCTG AGCCTCTGGA GAATGGGAGA 
GTTTTAGTCC AGGGCTCAAC CTTCACCTAC CAGGATATCC TAGCTGGGCT GGTTGGGTAT 
GTGCCTAGTG TCCCTGGCAT GGTCGTGGAT GAGTTCCAGT TCTCCCTCAC TGATGGCCTC 
CACGTGGACA CAGGGAGGAT GAAGATCTAC ACAGAACTGC CTGCAAGTGA CACACCTCAC 
TTGGCTATAA ACCAAGGCCT ACAGCTCTCA GCAGGGTCTG TAGCACGCAT CACAGAACAG 
CACTTGAAAG TGACAGATAT TGACTCAGAT GACCATCAGG TTATGTACAT CATGAAGGAA 
GATCCTGGTG CAGGGCGCCT GCAGATGATG AAGCATGGCA ACCTGGAGCA AATTTCTATT 
AAAGGCCCCA TCCGAAGTTT CACCCAGGCA GACATTAGCC AAGGCCACGT AGAATATAGT 
CATGGAACAG GAGAACCTGG AGGGAGCTTT GCTTTTAAAT TTGATGTGGT TGATGGAGAA 
GGCAACAGAT TGATTGACAA GTCATTTTCC ATCAGCATTC TAGAAGACAA ATCCCCACCA 
GTCATCACCA CCAATAAAGG ACTGGTCTTG GATGAAAACT CAGTGAAGAA AATCACCACC 
CTGCAGCTGT CTGCCACTGA CCAGGACAGT GGGCCTACAG AATTGATCTA CAGAATCACC 
AGACAGCCCC AGCTGGGCCA CTTGGAACAT GCAGCATCAC CAGGTATCCA GATTAGTTCC 
TTTACTCAAG CTGATCTGAC TTCACGAAAT GTTCAGTATG TCCATTCTAG TGAGGCTGAG 
AAACATTCAG ATGCCTTCAG CTTTACACTG TCTGATGGAG TCAGTGAGGT GACTCAGACT 
TTCCATATCA CTCTTCACCC TGTCGATGAT TCGCTGCCCG TCGTACAGAA CTTAGGAATG 
CGGGTGCAGG AGGGCATGAG GAAGACCATC ACAGAGTTTG AGCTTAAGGC GGTGGATGCT 
GACACAGAGA TTATGACAGC AGCACCTCAG CCGTTCCGAG TAGACATCCT CCCGGTAGAT 
GATGGCACGC CTAGAATTGT CACCAACCTG GGACTCCAGT GGCTGGAATA CATGGATGGC 
AAGGCAACCA ACCTGATCAC CAAGAAGGAA CTGCTGACCA TGGACCCAGA CACCGAGGAC 
GCGCAGCTTG TCTATGAGAT AACGACGGGC CCTAAGCATG GCTTTGTGGA GAACAAGCTG 
CAGCCTGGCA GAGCTGCTGC CACTTTCACC CAGGAGGATG TGAACTTGGG GTTGATTCGT 
TATGTGTTGC ACAAGGAGAA GATCCGTGAG ATGATGGATA GTTTTCAGTT TCTGGTGAAA 
GACAGTAAAC CCAATGTGGT CAGCGACAAT GTCTTCCATA TCCAGTGGTC ACTCATCAGC 
TTTAAATATA CCAGCTACAA TGTCAGTGAG AAGGCAGGGT CTGTCAGTGT CACGGTGCAG 
AGGACTGGGA ACCTGAACCA ATATGCCATC GTCCTGTGTC GCACCGAGCA AGGCACCGCC 
AGCTCCAGCT CCAGGGTCAG CTCCCAACCT GGGCAACAGG ACTATGTAGA GTATGCTGGC 
CAGGTCCAGT TTGATGAGCG AGAGGACACC AAGTCCTGCA CCATTGTCAT CAACGATGAT 
GACGTGTTTG AAAATGTTGA GAGTTTCACT GTGGAGCTCA GCATGCCAGC TTATGCCCTG 
TTAGGGGAAT TCACCCAGGC GAAGGTCATT ATCAACGATA CCGAGGATGA ACCCACATTA 
GAGTTTGACA AGAAGATCTA CTGGGTTAAC GAGAGCGCTG GTTTTCTGTT TGCACCTATT 
GAAAGAAAAG GAGATGCAAG CAGCATTGTA TCTGCAATTT GCTACACAGT CCCTAAGTCA 
GCTATGGGAA GTAGCCTCTA TGCTCTAGAA TCAGGCTCTG ATTTTAAATC TAGAGGGATG 
TCTGCCGCGA GTCGTGTGAT ATTCGGGCCT GGTGTGACCA TGTCCACCTG TGATGTCATG 
CTTATTGATG ACAGCGAGTA TGAAGAGGAA GAAGAGTTTG AGATTGCCTT GGCAGATGCC 
TCTGACAATG CCCGCATTGG AAGGGTGGCG ACAGCCAAGG TGCTCATTAG TGGTCCCAAC 
GATGCCTCGA CTGTGTCCCT GGGCAACACG GCTTTCACTG TCAGTGAGGA CGCAGGCACA 
GTAAAGATTC CAGTTATCCG CCATGGTACT GACCTCTCTA CTTTCGCATC TGTCTGGTGT 
GCAACGCGGC CCTCAGACCC AGCTTCTGCC ACACCAGGAG TTGACTACGT TCCCAGCTCT 
CGGAAGGTGG AATTTGGGCC TGGTGTCATT GAACAGTATT GCACCTTGAC TATCTTGGAT 
GACACTCAGT ATCCGGTAAT TGAAGGACTG GAGACATTTG TGGTTTTCCT CAGCTCAGCA 
CAAGGAGCCG AACTGACCAA ACCCTTCCAG GCAGTCATTG CAATTAATGA CACATTCCAA 
GATGTGCCCA GCATGCAGTT TGCCAAGGAT TTGCTCCTAG TGAAGGAGAA GGAGGGTGTC 
CTGCATGTCC CTATCACTCG GAGCGGAGAC CTGAGCTATG AGTCATCAGT GAGGTGCTAT 
ACTCAGAGCC ATTCCGCTCA GGTCATGGAG GACTTTGAGG AGAGACAAAA TGCAGACTCT 
TCACGGATTA CATTTCTCAA AGGGGACAAA GTGAAGAACT GTACGGTCTA TATCCACGAT 
GACTCCATGT TTGAGCCAGA GGAACAGTTC AGGGTCTACC TCGGCCTTCC TCTTGGAAAC 
CACTGGAGTG GAGCTAGAAT TGGAAAGAAT AACATGGCCA CCATCACCAT ATCCAATGAT 
GAAGATGCCC CCACCATTGA GTTTGAAGAA GCTGCATACC AAGTCCGGGA ACCCGCAGGC 
CCAGATGCCA TTGCGATTCT GAACATCAAG GTGATCCGCA GAGGGGATCA GAACAGGACC 
TCCAAGGTTC GCTGCAGCAC GCGGGATGGC TCTGCCCAGT CTGGTGTGGA TTATTACCCA 
AAGAGCCGAG TCTTGAAGTT CAGTCCCGGT GTGGATCATA TCTTTTTTAA AGTTGAGATC 
CTGTCCAATG AAGACCGGGA ATGGCATGAA TCTTTCTCAC TAGTCCTTGG CCCAGATGAC 
CCAGTGGAAG CAGTTCTTGG GGATGTGACT ACTGCCACGG TGACAATTCT AGACCAGGAG 
GCAGCAGGGA GCCTCATATT GCCAGCACCA CCCATTGTGG TCACACTTGC TGACTATGAC 
CATGTGGAAG AAGTTACCAA GGAAGGAGTC AAGAAATCCC CCTCCCCAGG CTACCCACTG 
GTCTGTGTCA CCCCCTGCGA CCCTCATTTC CCCAGATACG CTGTCATGAA GGAGCGCTGC 
AGTGAGGCCG GCATCAACCA GACATCTGTG CAGTTCAGCT GGGAAGTGGC TGCCCCCACT 
GATGGCAATG GGGCCCGGTC TCCCTTTGAA ACCATCACTG ACAACACACC ATTCACCAGT 
GTCAACCACA TGGTCCTGGA CAGCATTTAC TTCAGCCGGA GGTTCCATGT GCGTTGTGTG 
GCCAAGGCTG TGGACAAGGT GGGCCATGTG GGGACCCCCT TAAGGAGCAA CATTGTTACC 
ATTGGAACAG ACAGTGCTAT CTGCCACACA CCAGTGGTGG CTGGGACATC CAGAGGCTTC 
CAGGCTCAGT CCTTCATCGC AACCTTGAAA TACCTGGATG TCAAACATAA GGAGCATCCG 
AACAGAATCC ACATTTCGGT GCAGATCCCA CACCAGGATG GAATGCTGCC CCTTATCTCC 
ACCATGCCGT TGCACAACTT ACATTTTCTA CTGTCTGAGT CCATCTACAG ACACCAGCAC 
GTCTGCTCCA ATTTAGTTAC CACCTATGAC CTGAGAGGCA TCTCAGAGGC AGGGTTCCTG 
GATGATGTGG TCTATGATAG CACTGCCCTG GGGCCTGGCT ACGATCGCCC CTTCCAGTTT 
GACCCCAGCG TGCGAGAGCC GAAGACCATC CAGCTCTACA AACACCTGAA CCTGAAGAGC 
TGCGTGTGGA CCTTTGATGC TTATTATGAC ATGACTGAGC TGATTGACGT CTGTGGGGGC 
TCTGTAACCG CTGACTTCCA GGTGAGGGAC TCTGCCCAGT CCTTCTTGAC AGTGCACGTG 
CCTCTATATG TGTCCTACAT CTATGTGACA GCCCCCAGGG GCTGGGCCTC CTTGGAGCAC 
CACACCGAGA TGGAGTTTTC TTTCTTCTAT GACACTGTTC TCTGGAGAAC AGGAATCCAG 
ACAGACAGCG TGCTCTCTGC AAGGCTTCAG ATAATAAGAA TCTACATTCG AGAGGATGGC 
CGTCTTGTCA TTGAATTCAA GACCCATGCC AAATTCAGAG GACAGTTTGT GATGGAGCAT 
CACACTCTCC CAGAAGTGAA ATCTTTCGTA TTGACTCCAG ACCACCTAGG AGGAATTGAA 
TTTGACTTGC AGCTATTATG GAGCGCTCAG ACTTTTGATT CTCCACATCA ACTCTGGAGA 
GCCACAAGCT CTTATAACAG GAAGGACTAC TCAGGAGAGT ACACCATCTA CCTGATCCCT 
TGCACAGTGC AGCCCACACA GCCATGGGTT GACCCAGGAG AGAAGCCTTT GGCCTGCACT 
GCACATGCCC CAGAAAGATT CCTGATACCC ATTGCATTCC AGCAGACCAA CCGCCCTGTG 
CCAGTTGTGT ATTCACTTAA CACTGAATTT CAGCTCTGCA ATAATGAGAA GGTGTTCCTA 
ATGGATCCCA ATACATCTGA TATGTCACTA GCAGAAATGG ATTACAAAGG AGCCTTTTCA 
AAAGGTCAAA TCCTTTATGG CCGAGTACTT TGGAATCCAG AACAAAATCT TAATTCTGCT 
TACAAACTCC AGCTGGAGAA AGTCTATCTT TGTACGGGCA AGGATGGTTA TGTGCCTTTC 
TTTGATCCCA CGGGGACAAT CTACAATGAA GGGCCCCAGT ATGGATGCAT TCAGCCAAAC 
AAACACCTAA AACACAGATT CCTGCTGTTG GACCGCAATC AGCCAGAGGT AACTGATAAG 
TACTTCCATG ATGTGCCTTT TGAGGCTCAC TTTGCCTCTG AGTTGCCTGA TTTCCATGTG 
GTCAGTAACA TGCCAGGTGT GGATGGATTT ACTCTAAAAG TAGATGCACT CTATAAGGTG 
GAAGCAGGAC ACCAGTGGTA TCTCCAGGTC ATCTACATCA TTGGCCCTGA CACCATCTCA 
GGGCCCCGGG TCCAGCGCTC TCTCACAGCT CCACTCAGAC GCAACCGAAG GGACCTGGTA 
GAGCCCGATG GCCAGCTGAT CCTTGATGAT TCCCTCATCT ATGACAATGA AGGAGACCAA 
GTCAAGAATG GCACCAATAT GAAGTCCCTG AATCTGGAGA TGCAAGAGTT GGCGGTAGCT 
GCGTCCCTGT CACAGACTGG GGCGTCCATT GGCAGTGCCC TGGCTGCAAT CATGCTTCTA 
CTTCTGGTGT TTTTGGTGGC TTGTTTTATC AACAGGAAAT GCCAGAAACA GAGGAAGAAG 
AAGCCCGCAG AGGACATTTT GGAAGAATAT CCTCTGAATA CCAAGGTAGA AGTGCCCAAG 
AGGCACCCGG ACCGGGTGGA GAAGAACGTG AATAGACACT ACTGCACTGT GCGGAACGTC 
AACATCCTGA GTGAGCCTGA GGCGGCTTAC ACGTTCAAAG GTGCTAAAGT CAAAAGACTG 
AATCTAGAAG TCAGAGTTCA CAACAATTTA CAAGATGGAA CAGAAGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714379.1
CDS	1043..12853
Translation	MGVLKVWLGLALALAEFAVLPHHSEGACVYQDSLLADATIWKPDSCQSCRCHGDIVICKPAVCRNPQCAFEKGEVLQIAANQCCPECVLRTPGSCHHEKKIHEHGTEWASSPCSVCSCNHGEVRCTPQPCPPLSCGHQELAFIPEGSCCPVCVGLGKPCSYEGHVFQDGEDWRLSRCAKCLCRNGVAQCFTAQCQPLFCNQDETVVRVPGKCCPQCSARSCSAAGQVYEHGEQWSENACTTCICDRGEVRCHKQACLPLRCGKGQSRARRHGQCCEECVSPAGSCSYDGVVRYQDEMWKGSACEFCMCDHGQVTCQTGECAKVECARDEELIHLDGKCCPECISRNGYCVYEETGEFMSSNASEVKRIPEGEKWEDGPCKVCECRGAQVTCYEPSCPPCPVGTLALEVKGQCCPDCTSVHCHPDCLTCSQSPDHCDLCQDPTKLLQNGWCVHSCGLGFYQAGSLCLACQPQCSTCTSGLECSSCQPPLLMRHGQCVPTCGDGFYQDRHSCAVCHESCAGCWGPTEKHCLACRDPLHVLRDGGCESSCGKGFYNRQGTCSACDQSCDSCGPSSPRCLTCTEKTVLHDGKCMSECPGGYYADATGRCKVCHNSCASCSGPTPSHCTACSPPKALRQGHCLPRCGEGFYSDHGVCKACHSSCLACMGPAPSHCTGCKKPEEGLQVEQLSDVGIPSGECLAQCRAHFYLESTGICEACHQSCFRCAGKSPHNCTDCGPSHVLLDGQCLSQCPDGYFHQEGSCTECHPTCRQCHGPLESDCISCYPHISLTNGNCRTSCREEQFLNLVGYCADCHHLCQHCAADLHNTGSICLRCQNAHYLLLGDHCVPDCPSGYYAERGACKKCHSSCRTCQGRGPFSCSSCDTNLVLSHTGTCSTTCFPGHYLDDNHVCQPCNTHCGSCDSQASCTSCRDPNKVLLFGECQYESCAPQYYLDFSTNTCKECDWSCSACSGPLKTDCLQCMDGYVLQDGACVEQCLSSFYQDSGLCKNCDSYCLQCQGPHECTRCKGPFLLLEAQCVQECGKGYFADHAKHKCTACPQGCLQCSHRDRCHLCDHGFFLKSGLCVYNCVPGFSVHTSNETCSGKIHTPSLHVNGSLILPIGSIKPLDFSLLNVQDQEGRVEDLLFHVVSTPTNGQLVLSRNGKEVQLDKAGRFSWKDVNEKKVRFVHSKEKLRKGYLFLKISDQQFFSEPQLINIQAFSTQAPYVLRNEVLHISRGERATITTQMLDIRDDDNPQDVVIEIIDPPLHGQLLQTLQSPATPIYQFQLDELSRGLLHYAHDGSDSTSDVAVLQANDGHSFHNILFQVKTVPQNDRGLQLVANSMVWVPEGGMLQITNRILQAEAPGASAEEIIYKITQDYPQFGEVVLLVNMPADSPADEGQHLPDGRTATPTSTFTQQDINEGIVWYRHSGAPAQSDSFRFEVSSASNAQTRLESHMFNIAILPQTPEAPKVSLEASLHMTAREDGLTVIQPHSLSFINSEKPSGKIVYNITLPLHPNQGIIEHRDHPHSPIRYFTQEDINQGKVMYRPPPAAPHLQELMAFSFAGLPESVKFHFTVSDGEHTSPEMVLTIHLLPSDQQLPVFQVTAPRLAVSPGGSTSVGLQVVVRDAETAPKELFFELRRPPQHGVLLKHTAEFRRPMATGDTFTYEDVEKNALQYIHDGSSTREDSMEISVTDGLTVTMLEVRVEVSLSEDRGPRLAAGSSLSITVASKSTAIITRSHLAYVDDSSPDPEIWIQLNYLPSYGTLLRISGSEVEELSEVSNFTMEDINNKKIRYSAVFETDGHLVTDSFYFSVSDMDHNHLDNQIFTIMITPAENPPPVIAFADLITVDEGGRAPLSFHHFFATDDDDNLQRDAIIKLSALPKYGCIENTGTGDRFGPETASDLEASFPIQDVLENYIYYFQSVHESIEPTHDIFSFYVSDGTSRSEIHSINITIERKNDEPPRMTLQPLRVQLSSGVVISNSSLSLQDLDTPDNELIFVLTKKPDHGHVLWRQTASEPLENGRVLVQGSTFTYQDILAGLVGYVPSVPGMVVDEFQFSLTDGLHVDTGRMKIYTELPASDTPHLAINQGLQLSAGSVARITEQHLKVTDIDSDDHQVMYIMKEDPGAGRLQMMKHGNLEQISIKGPIRSFTQADISQGHVEYSHGTGEPGGSFAFKFDVVDGEGNRLIDKSFSISILEDKSPPVITTNKGLVLDENSVKKITTLQLSATDQDSGPTELIYRITRQPQLGHLEHAASPGIQISSFTQADLTSRNVQYVHSSEAEKHSDAFSFTLSDGVSEVTQTFHITLHPVDDSLPVVQNLGMRVQEGMRKTITEFELKAVDADTEIMTAAPQPFRVDILPVDDGTPRIVTNLGLQWLEYMDGKATNLITKKELLTMDPDTEDAQLVYEITTGPKHGFVENKLQPGRAAATFTQEDVNLGLIRYVLHKEKIREMMDSFQFLVKDSKPNVVSDNVFHIQWSLISFKYTSYNVSEKAGSVSVTVQRTGNLNQYAIVLCRTEQGTASSSSRVSSQPGQQDYVEYAGQVQFDEREDTKSCTIVINDDDVFENVESFTVELSMPAYALLGEFTQAKVIINDTEDEPTLEFDKKIYWVNESAGFLFAPIERKGDASSIVSAICYTVPKSAMGSSLYALESGSDFKSRGMSAASRVIFGPGVTMSTCDVMLIDDSEYEEEEEFEIALADASDNARIGRVATAKVLISGPNDASTVSLGNTAFTVSEDAGTVKIPVIRHGTDLSTFASVWCATRPSDPASATPGVDYVPSSRKVEFGPGVIEQYCTLTILDDTQYPVIEGLETFVVFLSSAQGAELTKPFQAVIAINDTFQDVPSMQFAKDLLLVKEKEGVLHVPITRSGDLSYESSVRCYTQSHSAQVMEDFEERQNADSSRITFLKGDKVKNCTVYIHDDSMFEPEEQFRVYLGLPLGNHWSGARIGKNNMATITISNDEDAPTIEFEEAAYQVREPAGPDAIAILNIKVIRRGDQNRTSKVRCSTRDGSAQSGVDYYPKSRVLKFSPGVDHIFFKVEILSNEDREWHESFSLVLGPDDPVEAVLGDVTTATVTILDQEAAGSLILPAPPIVVTLADYDHVEEVTKEGVKKSPSPGYPLVCVTPCDPHFPRYAVMKERCSEAGINQTSVQFSWEVAAPTDGNGARSPFETITDNTPFTSVNHMVLDSIYFSRRFHVRCVAKAVDKVGHVGTPLRSNIVTIGTDSAICHTPVVAGTSRGFQAQSFIATLKYLDVKHKEHPNRIHISVQIPHQDGMLPLISTMPLHNLHFLLSESIYRHQHVCSNLVTTYDLRGISEAGFLDDVVYDSTALGPGYDRPFQFDPSVREPKTIQLYKHLNLKSCVWTFDAYYDMTELIDVCGGSVTADFQVRDSAQSFLTVHVPLYVSYIYVTAPRGWASLEHHTEMEFSFFYDTVLWRTGIQTDSVLSARLQIIRIYIREDGRLVIEFKTHAKFRGQFVMEHHTLPEVKSFVLTPDHLGGIEFDLQLLWSAQTFDSPHQLWRATSSYNRKDYSGEYTIYLIPCTVQPTQPWVDPGEKPLACTAHAPERFLIPIAFQQTNRPVPVVYSLNTEFQLCNNEKVFLMDPNTSDMSLAEMDYKGAFSKGQILYGRVLWNPEQNLNSAYKLQLEKVYLCTGKDGYVPFFDPTGTIYNEGPQYGCIQPNKHLKHRFLLLDRNQPEVTDKYFHDVPFEAHFASELPDFHVVSNMPGVDGFTLKVDALYKVEAGHQWYLQVIYIIGPDTISGPRVQRSLTAPLRRNRRDLVEPDGQLILDDSLIYDNEGDQVKNGTNMKSLNLEMQELAVAASLSQTGASIGSALAAIMLLLLVFLVACFINRKCQKQRKKKPAEDILEEYPLNTKVEVPKRHPDRVEKNVNRHYCTVRNVNILSEPEAAYTFKGAKVKRLNLEVRVHNNLQDGTEV

Target ORF information:

RefSeq Version	XM_006714316.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714316.3

ORF Insert Sequence:

ATGGGTGTCC TCAAAGTGTG GCTCGGGCTG GCCCTAGCGT TGGCGGAATT TGCAGTATTG 
CCTCATCATT CCGAAGGTGC TTGTGTCTAT CAGGATTCCT TGTTGGCGGA TGCCACAATT 
TGGAAGCCCG ATTCATGCCA GAGCTGCCGT TGCCATGGTG ATATTGTTAT CTGCAAACCT 
GCTGTTTGCA GAAACCCTCA ATGTGCCTTT GAGAAGGGAG AAGTGCTTCA AATAGCTGCC 
AACCAATGCT GTCCTGAGTG TGTTTTGAGG ACTCCAGGAT CTTGCCATCA TGAAAAGAAA 
ATCCATGAGC ATGGGACAGA ATGGGCCTCT TCTCCATGTA GTGTGTGCTC TTGCAATCAT 
GGGGAAGTCC GATGTACCCC CCAACCATGC CCACCGCTGT CATGTGGACA CCAGGAGCTG 
GCATTCATCC CTGAAGGAAG CTGCTGCCCA GTTTGTGTGG GCCTTGGGAA ACCCTGTTCC 
TATGAAGGCC ATGTGTTTCA GGATGGGGAG GACTGGCGGC TGAGCCGGTG TGCCAAATGT 
CTGTGTAGAA ATGGGGTTGC CCAGTGCTTC ACAGCTCAGT GTCAGCCTCT ATTTTGTAAC 
CAGGATGAGA CTGTAGTCCG AGTCCCTGGA AAATGTTGCC CGCAGTGCTC TGCAAGATCC 
TGCTCTGCAG CTGGCCAAGT ATACGAGCAT GGTGAGCAGT GGAGCGAAAA TGCCTGCACC 
ACGTGTATAT GTGACCGGGG TGAGGTCAGG TGTCACAAGC AGGCCTGCCT GCCCCTGAGA 
TGCGGAAAGG GTCAGAGCAG GGCTCGGCGT CATGGGCAAT GCTGTGAGGA ATGTGTGTCT 
CCTGCCGGGA GCTGCTCCTA TGATGGAGTT GTGCGGTACC AGGACGAAAT GTGGAAGGGC 
TCGGCCTGTG AGTTCTGCAT GTGTGATCAT GGCCAAGTGA CCTGCCAGAC TGGAGAGTGT 
GCCAAAGTGG AGTGTGCCCG GGATGAAGAA TTAATTCACT TAGATGGAAA GTGTTGTCCT 
GAATGCATTT CAAGGAATGG TTATTGTGTT TATGAAGAAA CTGGAGAATT TATGTCATCA 
AATGCTAGTG AAGTTAAACG TATTCCAGAG GGAGAGAAGT GGGAAGATGG CCCTTGCAAG 
GTGTGTGAGT GCCGAGGGGC TCAGGTAACT TGCTACGAGC CCTCTTGCCC ACCATGTCCA 
GTGGGCACAC TGGCCTTAGA GGTGAAGGGA CAGTGCTGTC CAGACTGCAC ATCAGTTCAT 
TGCCATCCAG ATTGTTTGAC ATGCTCTCAG TCTCCAGACC ACTGTGACCT CTGCCAAGAT 
CCTACCAAGT TACTGCAGAA TGGATGGTGT GTGCACAGCT GTGGACTGGG TTTTTACCAA 
GCTGGCAGTC TCTGTTTAGC CTGCCAGCCC CAGTGCTCCA CGTGTACCAG TGGGCTGGAG 
TGCTCATCCT GCCAGCCTCC CCTGCTGATG CGGCACGGGC AGTGTGTGCC TACCTGTGGG 
GACGGCTTCT ACCAAGATCG CCATTCCTGT GCAGTCTGCC ATGAGTCCTG TGCAGGTTGC 
TGGGGCCCAA CGGAGAAGCA CTGCTTGGCC TGCAGAGATC CCCTCCACGT GCTGAGAGAT 
GGCGGCTGTG AGAGCAGCTG TGGAAAAGGC TTCTACAACA GGCAGGGCAC CTGTAGCGCT 
TGTGACCAAT CCTGTGACAG TTGTGGCCCC AGTAGCCCCA GGTGTCTTAC CTGTACTGAG 
AAGACAGTGC TGCATGATGG GAAATGCATG TCTGAATGCC CTGGCGGGTA CTATGCTGAT 
GCCACTGGCA GGTGCAAAGT TTGTCATAAC TCATGTGCCA GCTGCTCTGG GCCCACACCC 
TCTCACTGTA CAGCCTGCAG CCCCCCCAAG GCTCTGCGTC AAGGCCACTG TCTGCCCCGC 
TGTGGAGAGG GTTTCTACTC TGACCATGGA GTCTGCAAAG CCTGTCACTC CTCCTGCCTG 
GCTTGTATGG GTCCCGCACC CTCTCACTGT ACTGGGTGTA AGAAGCCAGA GGAAGGACTG 
CAAGTGGAGC AGCTGTCTGA CGTGGGCATC CCCTCTGGCG AGTGTCTAGC CCAGTGTAGA 
GCCCATTTTT ACTTGGAGAG CACTGGCATA TGTGAAGCTT GCCACCAGTC CTGTTTCAGA 
TGTGCAGGGA AAAGCCCACA TAACTGCACA GACTGTGGGC CTTCCCATGT GCTGTTGGAT 
GGGCAGTGCC TCTCCCAGTG CCCAGATGGC TACTTTCACC AGGAAGGTAG TTGCACAGAG 
TGTCACCCAA CCTGCAGGCA GTGTCATGGG CCGTTGGAGT CTGACTGCAT CTCCTGTTAC 
CCTCACATCT CTCTTACCAA TGGTAACTGC AGGACCAGCT GCAGGGAAGA GCAGTTCCTC 
AACCTCGTGG GATACTGTGC TGACTGCCAT CACCTGTGCC AGCACTGTGC AGCTGATCTC 
CACAACACTG GGAGCATCTG CCTCAGGTGC CAGAATGCCC ACTACCTGCT GCTCGGGGAC 
CACTGTGTTC CTGACTGCCC TTCAGGATAC TATGCAGAGA GAGGAGCTTG TAAAAAATGC 
CACTCCTCCT GCAGAACCTG CCAGGGCAGA GGACCTTTCT CCTGCTCCTC ATGTGACACC 
AACCTCGTGC TGTCCCACAC TGGCACCTGC AGCACCACCT GCTTCCCTGG GCACTATCTT 
GATGACAATC ATGTTTGCCA GCCATGCAAC ACACACTGTG GAAGCTGTGA TTCACAGGCC 
AGCTGTACCT CCTGCCGAGA TCCAAACAAG GTTCTGCTCT TTGGGGAATG TCAATACGAG 
AGCTGCGCCC CACAGTACTA TCTTGACTTC TCCACCAACA CGTGCAAAGA GTGTGATTGG 
AGCTGCAGTG CATGCAGTGG GCCCCTGAAA ACAGACTGCC TGCAGTGCAT GGATGGCTAT 
GTTCTCCAGG ATGGGGCCTG CGTGGAGCAG TGCTTGTCAT CATTTTACCA GGACTCGGGC 
CTCTGCAAGA ACTGTGACAG CTACTGTCTC CAGTGCCAAG GTCCCCATGA GTGTACCCGC 
TGCAAAGGGC CATTTCTCCT CTTGGAAGCC CAGTGTGTCC AGGAATGTGG GAAGGGGTAC 
TTTGCAGATC ATGCAAAGCA CAAATGCACA GCCTGCCCTC AGGGGTGCTT GCAGTGCAGC 
CACAGGGACC GTTGTCACCT CTGTGACCAT GGGTTCTTTC TGAAGAGTGG CCTCTGTGTT 
TACAACTGTG TTCCTGGCTT TTCTGTCCAC ACCTCTAATG AAACATGTTC TGGCAAAATA 
CACACCCCTA GTCTTCATGT GAATGGTTCC CTGATCCTCC CAATTGGTTC AATAAAGCCA 
CTGGATTTTT CCCTCCTGAA TGTCCAAGAC CAGGAGGGTA GGGTCGAAGA TCTCCTATTT 
CATGTTGTGA GCACTCCCAC CAATGGTCAG CTAGTGCTCT CAAGAAATGG AAAAGAGGTT 
CAGCTGGACA AGGCTGGCCG TTTTAGCTGG AAAGATGTGA ACGAGAAGAA AGTGCGTTTT 
GTGCACAGCA AAGAAAAACT CAGGAAAGGT TACCTTTTCC TGAAAATTAG TGACCAGCAG 
TTCTTCTCTG AGCCACAGCT GATCAACATA CAAGCATTTT CAACACAGGC CCCCTATGTG 
CTGAGAAATG AAGTTCTCCA CATTAGCAGA GGAGAGAGGG CAACCATCAC CACCCAGATG 
CTTGACATCC GAGATGATGA CAACCCACAG GATGTGGTCA TTGAAATAAT CGATCCTCCA 
CTTCATGGCC AATTGCTTCA GACACTTCAG TCCCCGGCAA CCCCTATCTA TCAATTCCAG 
CTGGATGAAC TCTCTAGAGG CCTTCTCCAC TATGCTCATG ATGGTTCAGA CAGCACATCC 
GATGTTGCAG TCTTGCAGGC CAATGATGGA CACTCCTTCC ATAATATACT GTTCCAAGTG 
AAGACCGTGC CTCAGAATGA CAGGGGTCTT CAGCTTGTGG CTAATTCGAT GGTGTGGGTT 
CCAGAAGGGG GGATGCTGCA GATCACCAAC AGAATCTTAC AGGCCGAGGC TCCTGGTGCC 
AGTGCTGAAG AAATCATCTA CAAGATTACA CAAGACTACC CCCAGTTTGG GGAGGTGGTC 
CTTCTAGTGA ATATGCCTGC AGACAGCCCT GCAGATGAAG GGCAGCACCT GCCTGATGGG 
AGGACAGCTA CCCCCACCAG CACCTTCACC CAGCAGGACA TCAATGAAGG CATCGTATGG 
TACAGGCACT CAGGAGCCCC AGCCCAGAGC GACTCCTTCC GCTTCGAGGT GTCCAGTGCC 
TCCAATGCCC AGACCCGCCT GGAGAGCCAC ATGTTCAACA TCGCGATCTT ACCACAGACA 
CCTGAAGCAC CTAAAGTGTC TCTGGAAGCA TCTCTCCATA TGACTGCTAG AGAAGATGGC 
CTGACTGTTA TTCAGCCTCA TTCCCTCTCC TTCATAAACT CTGAGAAGCC AAGTGGAAAG 
ATTGTCTACA ACATCACTCT ACCTCTGCAT CCAAATCAAG GTATCATCGA GCACCGGGAC 
CACCCTCACT CTCCTATCCG GTATTTCACG CAAGAGGATA TTAACCAGGG CAAAGTCATG 
TACCGCCCTC CCCCGGCAGC ACCCCACCTC CAGGAGCTCA TGGCCTTCTC GTTCGCTGGT 
CTCCCAGAAT CAGTGAAATT CCACTTCACA GTTTCAGATG GAGAACACAC AAGTCCGGAG 
ATGGTCCTCA CCATTCACTT ACTTCCCAGT GATCAGCAAC TGCCAGTGTT CCAGGTCACA 
GCTCCACGGC TGGCGGTCAG CCCAGGAGGC AGCACTTCTG TAGGACTTCA GGTGGTAGTA 
AGAGATGCTG AGACAGCGCC CAAAGAACTC TTCTTTGAGC TTCGGAGACC TCCACAGCAT 
GGTGTGCTTC TTAAGCATAC AGCTGAGTTC CGAAGGCCGA TGGCCACAGG TGACACTTTC 
ACCTATGAGG ATGTTGAGAA AAATGCTCTA CAGTATATAC ATGATGGTTC CTCTACCCGG 
GAAGACAGCA TGGAGATCTC AGTCACAGAT GGCCTCACAG TGACAATGCT GGAGGTGAGA 
GTAGAGGTGT CCCTGTCAGA AGACCGAGGG CCTCGACTGG CTGCTGGCTC CTCTCTGAGC 
ATTACTGTTG CCAGTAAAAG CACAGCCATA ATCACTAGGT CACACCTTGC TTACGTGGAT 
GATTCTTCCC CCGACCCAGA GATCTGGATT CAGTTAAATT ATCTGCCCTC ATATGGTACT 
CTCTTAAGAA TCTCAGGATC TGAGGTGGAA GAGCTCTCAG AAGTTTCCAA TTTCACAATG 
GAAGACATCA ATAACAAGAA AATCAGATAC TCAGCTGTGT TTGAAACTGA TGGTCATCTG 
GTTACTGATA GCTTCTATTT CTCTGTCTCT GACATGGACC ACAACCATCT GGATAATCAG 
ATATTTACCA TCATGATCAC TCCTGCTGAA AATCCACCTC CAGTCATTGC TTTTGCTGAC 
CTTATCACGG TTGATGAGGG AGGGAGAGCA CCACTCTCAT TTCACCATTT TTTTGCTACT 
GATGATGATG ACAACCTCCA GAGAGATGCC ATCATTAAAC TAAGTGCTCT GCCCAAATAT 
GGCTGCATTG AGAACACAGG AACAGGTGAT CGTTTTGGCC CTGAAACTGC CAGTGACCTA 
GAGGCATCAT TTCCTATTCA AGACGTCCTG GAAAACTACA TTTACTACTT TCAGAGTGTT 
CATGAAAGCA TTGAGCCAAC CCATGATATT TTTAGTTTTT ATGTGAGTGA TGGAACCAGT 
CGTTCAGAAA TTCACAGCAT CAATATCACC ATTGAGAGGA AGAACGATGA GCCTCCCAGG 
ATGACCTTGC AGCCCCTCAG AGTGCAGCTG AGCTCGGGAG TGGTGATAAG CAATTCTTCT 
TTGAGCCTGC AAGACCTGGA CACCCCAGAT AATGAGCTCA TTTTTGTATT GACAAAAAAG 
CCTGACCACG GTCATGTACT CTGGAGGCAA ACTGCTTCTG AGCCTCTGGA GAATGGGAGA 
GTTTTAGTCC AGGGCTCAAC CTTCACCTAC CAGGATATCC TAGCTGGGCT GGTTGGGTAT 
GTGCCTAGTG TCCCTGGCAT GGTCGTGGAT GAGTTCCAGT TCTCCCTCAC TGATGGCCTC 
CACGTGGACA CAGGGAGGAT GAAGATCTAC ACAGAACTGC CTGCAAGTGA CACACCTCAC 
TTGGCTATAA ACCAAGGCCT ACAGCTCTCA GCAGGGTCTG TAGCACGCAT CACAGAACAG 
CACTTGAAAG TGACAGATAT TGACTCAGAT GACCATCAGG TTATGTACAT CATGAAGGAA 
GATCCTGGTG CAGGGCGCCT GCAGATGATG AAGCATGGCA ACCTGGAGCA AATTTCTATT 
AAAGGCCCCA TCCGAAGTTT CACCCAGGCA GACATTAGCC AAGGCCACGT AGAATATAGT 
CATGGAACAG GAGAACCTGG AGGGAGCTTT GCTTTTAAAT TTGATGTGGT TGATGGAGAA 
GGCAACAGAT TGATTGACAA GTCATTTTCC ATCAGCATTC TAGAAGACAA ATCCCCACCA 
GTCATCACCA CCAATAAAGG ACTGGTCTTG GATGAAAACT CAGTGAAGAA AATCACCACC 
CTGCAGCTGT CTGCCACTGA CCAGGACAGT GGGCCTACAG AATTGATCTA CAGAATCACC 
AGACAGCCCC AGCTGGGCCA CTTGGAACAT GCAGCATCAC CAGGTATCCA GATTAGTTCC 
TTTACTCAAG CTGATCTGAC TTCACGAAAT GTTCAGTATG TCCATTCTAG TGAGGCTGAG 
AAACATTCAG ATGCCTTCAG CTTTACACTG TCTGATGGAG TCAGTGAGGT GACTCAGACT 
TTCCATATCA CTCTTCACCC TGTCGATGAT TCGCTGCCCG TCGTACAGAA CTTAGGAATG 
CGGGTGCAGG AGGGCATGAG GAAGACCATC ACAGAGTTTG AGCTTAAGGC GGTGGATGCT 
GACACAGAGA TTATGACAGC AGCACCTCAG CCGTTCCGAG TAGACATCCT CCCGGTAGAT 
GATGGCACGC CTAGAATTGT CACCAACCTG GGACTCCAGT GGCTGGAATA CATGGATGGC 
AAGGCAACCA ACCTGATCAC CAAGAAGGAA CTGCTGACCA TGGACCCAGA CACCGAGGAC 
GCGCAGCTTG TCTATGAGAT AACGACGGGC CCTAAGCATG GCTTTGTGGA GAACAAGCTG 
CAGCCTGGCA GAGCTGCTGC CACTTTCACC CAGGAGGATG TGAACTTGGG GTTGATTCGT 
TATGTGTTGC ACAAGGAGAA GATCCGTGAG ATGATGGATA GTTTTCAGTT TCTGGTGAAA 
GACAGTAAAC CCAATGTGGT CAGCGACAAT GTCTTCCATA TCCAGTGGTC ACTCATCAGC 
TTTAAATATA CCAGCTACAA TGTCAGTGAG AAGGCAGGGT CTGTCAGTGT CACGGTGCAG 
AGGACTGGGA ACCTGAACCA ATATGCCATC GTCCTGTGTC GCACCGAGCA AGGCACCGCC 
AGCTCCAGCT CCAGGGTCAG CTCCCAACCT GGGCAACAGG ACTATGTAGA GTATGCTGGC 
CAGGTCCAGT TTGATGAGCG AGAGGACACC AAGTCCTGCA CCATTGTCAT CAACGATGAT 
GACGTGTTTG AAAATGTTGA GAGTTTCACT GTGGAGCTCA GCATGCCAGC TTATGCCCTG 
TTAGGGGAAT TCACCCAGGC GAAGGTCATT ATCAACGATA CCGAGGATGA ACCCACATTA 
GAGTTTGACA AGAAGATCTA CTGGGTTAAC GAGAGCGCTG GTTTTCTGTT TGCACCTATT 
GAAAGAAAAG GAGATGCAAG CAGCATTGTA TCTGCAATTT GCTACACAGT CCCTAAGTCA 
GCTATGGGAA GTAGCCTCTA TGCTCTAGAA TCAGGCTCTG ATTTTAAATC TAGAGGGATG 
TCTGCCGCGA GTCGTGTGAT ATTCGGGCCT GGTGTGACCA TGTCCACCTG TGATGTCATG 
CTTATTGATG ACAGCGAGTA TGAAGAGGAA GAAGAGTTTG AGATTGCCTT GGCAGATGCC 
TCTGACAATG CCCGCATTGG AAGGGTGGCG ACAGCCAAGG TGCTCATTAG TGGTCCCAAC 
GATGCCTCGA CTGTGTCCCT GGGCAACACG GCTTTCACTG TCAGTGAGGA CGCAGGCACA 
GTAAAGATTC CAGTTATCCG CCATGGTACT GACCTCTCTA CTTTCGCATC TGTCTGGTGT 
GCAACGCGGC CCTCAGACCC AGCTTCTGCC ACACCAGGAG TTGACTACGT TCCCAGCTCT 
CGGAAGGTGG AATTTGGGCC TGGTGTCATT GAACAGTATT GCACCTTGAC TATCTTGGAT 
GACACTCAGT ATCCGGTAAT TGAAGGACTG GAGACATTTG TGGTTTTCCT CAGCTCAGCA 
CAAGGAGCCG AACTGACCAA ACCCTTCCAG GCAGTCATTG CAATTAATGA CACATTCCAA 
GATGTGCCCA GCATGCAGTT TGCCAAGGAT TTGCTCCTAG TGAAGGAGAA GGAGGGTGTC 
CTGCATGTCC CTATCACTCG GAGCGGAGAC CTGAGCTATG AGTCATCAGT GAGGTGCTAT 
ACTCAGAGCC ATTCCGCTCA GGTCATGGAG GACTTTGAGG AGAGACAAAA TGCAGACTCT 
TCACGGATTA CATTTCTCAA AGGGGACAAA GTGAAGAACT GTACGGTCTA TATCCACGAT 
GACTCCATGT TTGAGCCAGA GGAACAGTTC AGGGTCTACC TCGGCCTTCC TCTTGGAAAC 
CACTGGAGTG GAGCTAGAAT TGGAAAGAAT AACATGGCCA CCATCACCAT ATCCAATGAT 
GAAGATGCCC CCACCATTGA GTTTGAAGAA GCTGCATACC AAGTCCGGGA ACCCGCAGGC 
CCAGATGCCA TTGCGATTCT GAACATCAAG GTGATCCGCA GAGGGGATCA GAACAGGACC 
TCCAAGGTTC GCTGCAGCAC GCGGGATGGC TCTGCCCAGT CTGGTGTGGA TTATTACCCA 
AAGAGCCGAG TCTTGAAGTT CAGTCCCGGT GTGGATCATA TCTTTTTTAA AGTTGAGATC 
CTGTCCAATG AAGACCGGGA ATGGCATGAA TCTTTCTCAC TAGTCCTTGG CCCAGATGAC 
CCAGTGGAAG CAGTTCTTGG GGATGTGACT ACTGCCACGG TGACAATTCT AGACCAGGAG 
GCAGCAGGGA GCCTCATATT GCCAGCACCA CCCATTGTGG TCACACTTGC TGACTATGAC 
CATGTGGAAG AAGTTACCAA GGAAGGAGTC AAGAAATCCC CCTCCCCAGG CTACCCACTG 
GTCTGTGTCA CCCCCTGCGA CCCTCATTTC CCCAGATACG CTGTCATGAA GGAGCGCTGC 
AGTGAGGCCG GCATCAACCA GACATCTGTG CAGTTCAGCT GGGAAGTGGC TGCCCCCACT 
GATGGCAATG GGGCCCGGTC TCCCTTTGAA ACCATCACTG ACAACACACC ATTCACCAGT 
GTCAACCACA TGGTCCTGGA CAGCATTTAC TTCAGCCGGA GGTTCCATGT GCGTTGTGTG 
GCCAAGGCTG TGGACAAGGT GGGCCATGTG GGGACCCCCT TAAGGAGCAA CATTGTTACC 
ATTGGAACAG ACAGTGCTAT CTGCCACACA CCAGTGGTGG CTGGGACATC CAGAGGCTTC 
CAGGCTCAGT CCTTCATCGC AACCTTGAAA TACCTGGATG TCAAACATAA GGAGCATCCG 
AACAGAATCC ACATTTCGGT GCAGATCCCA CACCAGGATG GAATGCTGCC CCTTATCTCC 
ACCATGCCGT TGCACAACTT ACATTTTCTA CTGTCTGAGT CCATCTACAG ACACCAGCAC 
GTCTGCTCCA ATTTAGTTAC CACCTATGAC CTGAGAGGCA TCTCAGAGGC AGGGTTCCTG 
GATGATGTGG TCTATGATAG CACTGCCCTG GGGCCTGGCT ACGATCGCCC CTTCCAGTTT 
GACCCCAGCG TGCGAGAGCC GAAGACCATC CAGCTCTACA AACACCTGAA CCTGAAGAGC 
TGCGTGTGGA CCTTTGATGC TTATTATGAC ATGACTGAGC TGATTGACGT CTGTGGGGGC 
TCTGTAACCG CTGACTTCCA GGTGAGGGAC TCTGCCCAGT CCTTCTTGAC AGTGCACGTG 
CCTCTATATG TGTCCTACAT CTATGTGACA GCCCCCAGGG GCTGGGCCTC CTTGGAGCAC 
CACACCGAGA TGGAGTTTTC TTTCTTCTAT GACACTGTTC TCTGGAGAAC AGGAATCCAG 
ACAGACAGCG TGCTCTCTGC AAGGCTTCAG ATAATAAGAA TCTACATTCG AGAGGATGGC 
CGTCTTGTCA TTGAATTCAA GACCCATGCC AAATTCAGAG GACAGTTTGT GATGGAGCAT 
CACACTCTCC CAGAAGTGAA ATCTTTCGTA TTGACTCCAG ACCACCTAGG AGGAATTGAA 
TTTGACTTGC AGCTATTATG GAGCGCTCAG ACTTTTGATT CTCCACATCA ACTCTGGAGA 
GCCACAAGCT CTTATAACAG GAAGGACTAC TCAGGAGAGT ACACCATCTA CCTGATCCCT 
TGCACAGTGC AGCCCACACA GCCATGGGTT GACCCAGGAG AGAAGCCTTT GGCCTGCACT 
GCACATGCCC CAGAAAGATT CCTGATACCC ATTGCATTCC AGCAGACCAA CCGCCCTGTG 
CCAGTTGTGT ATTCACTTAA CACTGAATTT CAGCTCTGCA ATAATGAGAA GGTGTTCCTA 
ATGGATCCCA ATACATCTGA TATGTCACTA GCAGAAATGG ATTACAAAGG AGCCTTTTCA 
AAAGGTCAAA TCCTTTATGG CCGAGTACTT TGGAATCCAG AACAAAATCT TAATTCTGCT 
TACAAACTCC AGCTGGAGAA AGTCTATCTT TGTACGGGCA AGGATGGTTA TGTGCCTTTC 
TTTGATCCCA CGGGGACAAT CTACAATGAA GGGCCCCAGT ATGGATGCAT TCAGCCAAAC 
AAACACCTAA AACACAGATT CCTGCTGTTG GACCGCAATC AGCCAGAGGT AACTGATAAG 
TACTTCCATG ATGTGCCTTT TGAGGCTCAC TTTGCCTCTG AGTTGCCTGA TTTCCATGTG 
GTCAGTAACA TGCCAGGTGT GGATGGATTT ACTCTAAAAG TAGATGCACT CTATAAGGTG 
GAAGCAGGAC ACCAGTGGTA TCTCCAGGTC ATCTACATCA TTGGCCCTGA CACCATCTCA 
GGGCCCCGGG TCCAGCGCTC TCTCACAGCT CCACTCAGAC GCAACCGAAG GGACCTGGTA 
GAGCCCGATG GCCAGCTGAT CCTTGATGAT TCCCTCATCT ATGACAATGA AGGAGACCAA 
GTCAAGAATG GCACCAATAT GAAGTCCCTG AATCTGGAGA TGCAAGAGTT GGCGGTAGCT 
GCGTCCCTGT CACAGACTGG GGCGTCCATT GGCAGTGCCC TGGCTGCAAT CATGCTTCTA 
CTTCTGGTGT TTTTGGTGGC TTGTTTTATC AACAGGAAAT GCCAGAAACA GAGGAAGAAG 
AAGCCCGCAG AGGACATTTT GGAAGAATAT CCTCTGAATA CCAAGGTAGA AGTGCCCAAG 
AGGCACCCGG ACCGGGTGGA GAAGAACGTG AATAGACACT ACTGCACTGT GCGGAACGTC 
AACATCCTGA GTGAGCCTGA GGCGGCTTAC ACGTTCAAAG GTGCTAAAGT CAAAAGACTG 
AATCTAGAAG TCAGAGTTCA CAACAATTTA CAAGATGGAA CAGAAGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FRAS1 ( XM_006714316.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714379.1 Homo sapiens Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant X1, mRNA.

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CloneID	OHu39089
Clone ID Related Accession (Same CDS sequence)	XM_006714316.3 , XM_006714316.2
Accession Version	XM_006714316.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	extracellular matrix protein FRAS1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714316.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGTCC TCAAAGTGTG GCTCGGGCTG GCCCTAGCGT TGGCGGAATT TGCAGTATTG 
CCTCATCATT CCGAAGGTGC TTGTGTCTAT CAGGATTCCT TGTTGGCGGA TGCCACAATT 
TGGAAGCCCG ATTCATGCCA GAGCTGCCGT TGCCATGGTG ATATTGTTAT CTGCAAACCT 
GCTGTTTGCA GAAACCCTCA ATGTGCCTTT GAGAAGGGAG AAGTGCTTCA AATAGCTGCC 
AACCAATGCT GTCCTGAGTG TGTTTTGAGG ACTCCAGGAT CTTGCCATCA TGAAAAGAAA 
ATCCATGAGC ATGGGACAGA ATGGGCCTCT TCTCCATGTA GTGTGTGCTC TTGCAATCAT 
GGGGAAGTCC GATGTACCCC CCAACCATGC CCACCGCTGT CATGTGGACA CCAGGAGCTG 
GCATTCATCC CTGAAGGAAG CTGCTGCCCA GTTTGTGTGG GCCTTGGGAA ACCCTGTTCC 
TATGAAGGCC ATGTGTTTCA GGATGGGGAG GACTGGCGGC TGAGCCGGTG TGCCAAATGT 
CTGTGTAGAA ATGGGGTTGC CCAGTGCTTC ACAGCTCAGT GTCAGCCTCT ATTTTGTAAC 
CAGGATGAGA CTGTAGTCCG AGTCCCTGGA AAATGTTGCC CGCAGTGCTC TGCAAGATCC 
TGCTCTGCAG CTGGCCAAGT ATACGAGCAT GGTGAGCAGT GGAGCGAAAA TGCCTGCACC 
ACGTGTATAT GTGACCGGGG TGAGGTCAGG TGTCACAAGC AGGCCTGCCT GCCCCTGAGA 
TGCGGAAAGG GTCAGAGCAG GGCTCGGCGT CATGGGCAAT GCTGTGAGGA ATGTGTGTCT 
CCTGCCGGGA GCTGCTCCTA TGATGGAGTT GTGCGGTACC AGGACGAAAT GTGGAAGGGC 
TCGGCCTGTG AGTTCTGCAT GTGTGATCAT GGCCAAGTGA CCTGCCAGAC TGGAGAGTGT 
GCCAAAGTGG AGTGTGCCCG GGATGAAGAA TTAATTCACT TAGATGGAAA GTGTTGTCCT 
GAATGCATTT CAAGGAATGG TTATTGTGTT TATGAAGAAA CTGGAGAATT TATGTCATCA 
AATGCTAGTG AAGTTAAACG TATTCCAGAG GGAGAGAAGT GGGAAGATGG CCCTTGCAAG 
GTGTGTGAGT GCCGAGGGGC TCAGGTAACT TGCTACGAGC CCTCTTGCCC ACCATGTCCA 
GTGGGCACAC TGGCCTTAGA GGTGAAGGGA CAGTGCTGTC CAGACTGCAC ATCAGTTCAT 
TGCCATCCAG ATTGTTTGAC ATGCTCTCAG TCTCCAGACC ACTGTGACCT CTGCCAAGAT 
CCTACCAAGT TACTGCAGAA TGGATGGTGT GTGCACAGCT GTGGACTGGG TTTTTACCAA 
GCTGGCAGTC TCTGTTTAGC CTGCCAGCCC CAGTGCTCCA CGTGTACCAG TGGGCTGGAG 
TGCTCATCCT GCCAGCCTCC CCTGCTGATG CGGCACGGGC AGTGTGTGCC TACCTGTGGG 
GACGGCTTCT ACCAAGATCG CCATTCCTGT GCAGTCTGCC ATGAGTCCTG TGCAGGTTGC 
TGGGGCCCAA CGGAGAAGCA CTGCTTGGCC TGCAGAGATC CCCTCCACGT GCTGAGAGAT 
GGCGGCTGTG AGAGCAGCTG TGGAAAAGGC TTCTACAACA GGCAGGGCAC CTGTAGCGCT 
TGTGACCAAT CCTGTGACAG TTGTGGCCCC AGTAGCCCCA GGTGTCTTAC CTGTACTGAG 
AAGACAGTGC TGCATGATGG GAAATGCATG TCTGAATGCC CTGGCGGGTA CTATGCTGAT 
GCCACTGGCA GGTGCAAAGT TTGTCATAAC TCATGTGCCA GCTGCTCTGG GCCCACACCC 
TCTCACTGTA CAGCCTGCAG CCCCCCCAAG GCTCTGCGTC AAGGCCACTG TCTGCCCCGC 
TGTGGAGAGG GTTTCTACTC TGACCATGGA GTCTGCAAAG CCTGTCACTC CTCCTGCCTG 
GCTTGTATGG GTCCCGCACC CTCTCACTGT ACTGGGTGTA AGAAGCCAGA GGAAGGACTG 
CAAGTGGAGC AGCTGTCTGA CGTGGGCATC CCCTCTGGCG AGTGTCTAGC CCAGTGTAGA 
GCCCATTTTT ACTTGGAGAG CACTGGCATA TGTGAAGCTT GCCACCAGTC CTGTTTCAGA 
TGTGCAGGGA AAAGCCCACA TAACTGCACA GACTGTGGGC CTTCCCATGT GCTGTTGGAT 
GGGCAGTGCC TCTCCCAGTG CCCAGATGGC TACTTTCACC AGGAAGGTAG TTGCACAGAG 
TGTCACCCAA CCTGCAGGCA GTGTCATGGG CCGTTGGAGT CTGACTGCAT CTCCTGTTAC 
CCTCACATCT CTCTTACCAA TGGTAACTGC AGGACCAGCT GCAGGGAAGA GCAGTTCCTC 
AACCTCGTGG GATACTGTGC TGACTGCCAT CACCTGTGCC AGCACTGTGC AGCTGATCTC 
CACAACACTG GGAGCATCTG CCTCAGGTGC CAGAATGCCC ACTACCTGCT GCTCGGGGAC 
CACTGTGTTC CTGACTGCCC TTCAGGATAC TATGCAGAGA GAGGAGCTTG TAAAAAATGC 
CACTCCTCCT GCAGAACCTG CCAGGGCAGA GGACCTTTCT CCTGCTCCTC ATGTGACACC 
AACCTCGTGC TGTCCCACAC TGGCACCTGC AGCACCACCT GCTTCCCTGG GCACTATCTT 
GATGACAATC ATGTTTGCCA GCCATGCAAC ACACACTGTG GAAGCTGTGA TTCACAGGCC 
AGCTGTACCT CCTGCCGAGA TCCAAACAAG GTTCTGCTCT TTGGGGAATG TCAATACGAG 
AGCTGCGCCC CACAGTACTA TCTTGACTTC TCCACCAACA CGTGCAAAGA GTGTGATTGG 
AGCTGCAGTG CATGCAGTGG GCCCCTGAAA ACAGACTGCC TGCAGTGCAT GGATGGCTAT 
GTTCTCCAGG ATGGGGCCTG CGTGGAGCAG TGCTTGTCAT CATTTTACCA GGACTCGGGC 
CTCTGCAAGA ACTGTGACAG CTACTGTCTC CAGTGCCAAG GTCCCCATGA GTGTACCCGC 
TGCAAAGGGC CATTTCTCCT CTTGGAAGCC CAGTGTGTCC AGGAATGTGG GAAGGGGTAC 
TTTGCAGATC ATGCAAAGCA CAAATGCACA GCCTGCCCTC AGGGGTGCTT GCAGTGCAGC 
CACAGGGACC GTTGTCACCT CTGTGACCAT GGGTTCTTTC TGAAGAGTGG CCTCTGTGTT 
TACAACTGTG TTCCTGGCTT TTCTGTCCAC ACCTCTAATG AAACATGTTC TGGCAAAATA 
CACACCCCTA GTCTTCATGT GAATGGTTCC CTGATCCTCC CAATTGGTTC AATAAAGCCA 
CTGGATTTTT CCCTCCTGAA TGTCCAAGAC CAGGAGGGTA GGGTCGAAGA TCTCCTATTT 
CATGTTGTGA GCACTCCCAC CAATGGTCAG CTAGTGCTCT CAAGAAATGG AAAAGAGGTT 
CAGCTGGACA AGGCTGGCCG TTTTAGCTGG AAAGATGTGA ACGAGAAGAA AGTGCGTTTT 
GTGCACAGCA AAGAAAAACT CAGGAAAGGT TACCTTTTCC TGAAAATTAG TGACCAGCAG 
TTCTTCTCTG AGCCACAGCT GATCAACATA CAAGCATTTT CAACACAGGC CCCCTATGTG 
CTGAGAAATG AAGTTCTCCA CATTAGCAGA GGAGAGAGGG CAACCATCAC CACCCAGATG 
CTTGACATCC GAGATGATGA CAACCCACAG GATGTGGTCA TTGAAATAAT CGATCCTCCA 
CTTCATGGCC AATTGCTTCA GACACTTCAG TCCCCGGCAA CCCCTATCTA TCAATTCCAG 
CTGGATGAAC TCTCTAGAGG CCTTCTCCAC TATGCTCATG ATGGTTCAGA CAGCACATCC 
GATGTTGCAG TCTTGCAGGC CAATGATGGA CACTCCTTCC ATAATATACT GTTCCAAGTG 
AAGACCGTGC CTCAGAATGA CAGGGGTCTT CAGCTTGTGG CTAATTCGAT GGTGTGGGTT 
CCAGAAGGGG GGATGCTGCA GATCACCAAC AGAATCTTAC AGGCCGAGGC TCCTGGTGCC 
AGTGCTGAAG AAATCATCTA CAAGATTACA CAAGACTACC CCCAGTTTGG GGAGGTGGTC 
CTTCTAGTGA ATATGCCTGC AGACAGCCCT GCAGATGAAG GGCAGCACCT GCCTGATGGG 
AGGACAGCTA CCCCCACCAG CACCTTCACC CAGCAGGACA TCAATGAAGG CATCGTATGG 
TACAGGCACT CAGGAGCCCC AGCCCAGAGC GACTCCTTCC GCTTCGAGGT GTCCAGTGCC 
TCCAATGCCC AGACCCGCCT GGAGAGCCAC ATGTTCAACA TCGCGATCTT ACCACAGACA 
CCTGAAGCAC CTAAAGTGTC TCTGGAAGCA TCTCTCCATA TGACTGCTAG AGAAGATGGC 
CTGACTGTTA TTCAGCCTCA TTCCCTCTCC TTCATAAACT CTGAGAAGCC AAGTGGAAAG 
ATTGTCTACA ACATCACTCT ACCTCTGCAT CCAAATCAAG GTATCATCGA GCACCGGGAC 
CACCCTCACT CTCCTATCCG GTATTTCACG CAAGAGGATA TTAACCAGGG CAAAGTCATG 
TACCGCCCTC CCCCGGCAGC ACCCCACCTC CAGGAGCTCA TGGCCTTCTC GTTCGCTGGT 
CTCCCAGAAT CAGTGAAATT CCACTTCACA GTTTCAGATG GAGAACACAC AAGTCCGGAG 
ATGGTCCTCA CCATTCACTT ACTTCCCAGT GATCAGCAAC TGCCAGTGTT CCAGGTCACA 
GCTCCACGGC TGGCGGTCAG CCCAGGAGGC AGCACTTCTG TAGGACTTCA GGTGGTAGTA 
AGAGATGCTG AGACAGCGCC CAAAGAACTC TTCTTTGAGC TTCGGAGACC TCCACAGCAT 
GGTGTGCTTC TTAAGCATAC AGCTGAGTTC CGAAGGCCGA TGGCCACAGG TGACACTTTC 
ACCTATGAGG ATGTTGAGAA AAATGCTCTA CAGTATATAC ATGATGGTTC CTCTACCCGG 
GAAGACAGCA TGGAGATCTC AGTCACAGAT GGCCTCACAG TGACAATGCT GGAGGTGAGA 
GTAGAGGTGT CCCTGTCAGA AGACCGAGGG CCTCGACTGG CTGCTGGCTC CTCTCTGAGC 
ATTACTGTTG CCAGTAAAAG CACAGCCATA ATCACTAGGT CACACCTTGC TTACGTGGAT 
GATTCTTCCC CCGACCCAGA GATCTGGATT CAGTTAAATT ATCTGCCCTC ATATGGTACT 
CTCTTAAGAA TCTCAGGATC TGAGGTGGAA GAGCTCTCAG AAGTTTCCAA TTTCACAATG 
GAAGACATCA ATAACAAGAA AATCAGATAC TCAGCTGTGT TTGAAACTGA TGGTCATCTG 
GTTACTGATA GCTTCTATTT CTCTGTCTCT GACATGGACC ACAACCATCT GGATAATCAG 
ATATTTACCA TCATGATCAC TCCTGCTGAA AATCCACCTC CAGTCATTGC TTTTGCTGAC 
CTTATCACGG TTGATGAGGG AGGGAGAGCA CCACTCTCAT TTCACCATTT TTTTGCTACT 
GATGATGATG ACAACCTCCA GAGAGATGCC ATCATTAAAC TAAGTGCTCT GCCCAAATAT 
GGCTGCATTG AGAACACAGG AACAGGTGAT CGTTTTGGCC CTGAAACTGC CAGTGACCTA 
GAGGCATCAT TTCCTATTCA AGACGTCCTG GAAAACTACA TTTACTACTT TCAGAGTGTT 
CATGAAAGCA TTGAGCCAAC CCATGATATT TTTAGTTTTT ATGTGAGTGA TGGAACCAGT 
CGTTCAGAAA TTCACAGCAT CAATATCACC ATTGAGAGGA AGAACGATGA GCCTCCCAGG 
ATGACCTTGC AGCCCCTCAG AGTGCAGCTG AGCTCGGGAG TGGTGATAAG CAATTCTTCT 
TTGAGCCTGC AAGACCTGGA CACCCCAGAT AATGAGCTCA TTTTTGTATT GACAAAAAAG 
CCTGACCACG GTCATGTACT CTGGAGGCAA ACTGCTTCTG AGCCTCTGGA GAATGGGAGA 
GTTTTAGTCC AGGGCTCAAC CTTCACCTAC CAGGATATCC TAGCTGGGCT GGTTGGGTAT 
GTGCCTAGTG TCCCTGGCAT GGTCGTGGAT GAGTTCCAGT TCTCCCTCAC TGATGGCCTC 
CACGTGGACA CAGGGAGGAT GAAGATCTAC ACAGAACTGC CTGCAAGTGA CACACCTCAC 
TTGGCTATAA ACCAAGGCCT ACAGCTCTCA GCAGGGTCTG TAGCACGCAT CACAGAACAG 
CACTTGAAAG TGACAGATAT TGACTCAGAT GACCATCAGG TTATGTACAT CATGAAGGAA 
GATCCTGGTG CAGGGCGCCT GCAGATGATG AAGCATGGCA ACCTGGAGCA AATTTCTATT 
AAAGGCCCCA TCCGAAGTTT CACCCAGGCA GACATTAGCC AAGGCCACGT AGAATATAGT 
CATGGAACAG GAGAACCTGG AGGGAGCTTT GCTTTTAAAT TTGATGTGGT TGATGGAGAA 
GGCAACAGAT TGATTGACAA GTCATTTTCC ATCAGCATTC TAGAAGACAA ATCCCCACCA 
GTCATCACCA CCAATAAAGG ACTGGTCTTG GATGAAAACT CAGTGAAGAA AATCACCACC 
CTGCAGCTGT CTGCCACTGA CCAGGACAGT GGGCCTACAG AATTGATCTA CAGAATCACC 
AGACAGCCCC AGCTGGGCCA CTTGGAACAT GCAGCATCAC CAGGTATCCA GATTAGTTCC 
TTTACTCAAG CTGATCTGAC TTCACGAAAT GTTCAGTATG TCCATTCTAG TGAGGCTGAG 
AAACATTCAG ATGCCTTCAG CTTTACACTG TCTGATGGAG TCAGTGAGGT GACTCAGACT 
TTCCATATCA CTCTTCACCC TGTCGATGAT TCGCTGCCCG TCGTACAGAA CTTAGGAATG 
CGGGTGCAGG AGGGCATGAG GAAGACCATC ACAGAGTTTG AGCTTAAGGC GGTGGATGCT 
GACACAGAGA TTATGACAGC AGCACCTCAG CCGTTCCGAG TAGACATCCT CCCGGTAGAT 
GATGGCACGC CTAGAATTGT CACCAACCTG GGACTCCAGT GGCTGGAATA CATGGATGGC 
AAGGCAACCA ACCTGATCAC CAAGAAGGAA CTGCTGACCA TGGACCCAGA CACCGAGGAC 
GCGCAGCTTG TCTATGAGAT AACGACGGGC CCTAAGCATG GCTTTGTGGA GAACAAGCTG 
CAGCCTGGCA GAGCTGCTGC CACTTTCACC CAGGAGGATG TGAACTTGGG GTTGATTCGT 
TATGTGTTGC ACAAGGAGAA GATCCGTGAG ATGATGGATA GTTTTCAGTT TCTGGTGAAA 
GACAGTAAAC CCAATGTGGT CAGCGACAAT GTCTTCCATA TCCAGTGGTC ACTCATCAGC 
TTTAAATATA CCAGCTACAA TGTCAGTGAG AAGGCAGGGT CTGTCAGTGT CACGGTGCAG 
AGGACTGGGA ACCTGAACCA ATATGCCATC GTCCTGTGTC GCACCGAGCA AGGCACCGCC 
AGCTCCAGCT CCAGGGTCAG CTCCCAACCT GGGCAACAGG ACTATGTAGA GTATGCTGGC 
CAGGTCCAGT TTGATGAGCG AGAGGACACC AAGTCCTGCA CCATTGTCAT CAACGATGAT 
GACGTGTTTG AAAATGTTGA GAGTTTCACT GTGGAGCTCA GCATGCCAGC TTATGCCCTG 
TTAGGGGAAT TCACCCAGGC GAAGGTCATT ATCAACGATA CCGAGGATGA ACCCACATTA 
GAGTTTGACA AGAAGATCTA CTGGGTTAAC GAGAGCGCTG GTTTTCTGTT TGCACCTATT 
GAAAGAAAAG GAGATGCAAG CAGCATTGTA TCTGCAATTT GCTACACAGT CCCTAAGTCA 
GCTATGGGAA GTAGCCTCTA TGCTCTAGAA TCAGGCTCTG ATTTTAAATC TAGAGGGATG 
TCTGCCGCGA GTCGTGTGAT ATTCGGGCCT GGTGTGACCA TGTCCACCTG TGATGTCATG 
CTTATTGATG ACAGCGAGTA TGAAGAGGAA GAAGAGTTTG AGATTGCCTT GGCAGATGCC 
TCTGACAATG CCCGCATTGG AAGGGTGGCG ACAGCCAAGG TGCTCATTAG TGGTCCCAAC 
GATGCCTCGA CTGTGTCCCT GGGCAACACG GCTTTCACTG TCAGTGAGGA CGCAGGCACA 
GTAAAGATTC CAGTTATCCG CCATGGTACT GACCTCTCTA CTTTCGCATC TGTCTGGTGT 
GCAACGCGGC CCTCAGACCC AGCTTCTGCC ACACCAGGAG TTGACTACGT TCCCAGCTCT 
CGGAAGGTGG AATTTGGGCC TGGTGTCATT GAACAGTATT GCACCTTGAC TATCTTGGAT 
GACACTCAGT ATCCGGTAAT TGAAGGACTG GAGACATTTG TGGTTTTCCT CAGCTCAGCA 
CAAGGAGCCG AACTGACCAA ACCCTTCCAG GCAGTCATTG CAATTAATGA CACATTCCAA 
GATGTGCCCA GCATGCAGTT TGCCAAGGAT TTGCTCCTAG TGAAGGAGAA GGAGGGTGTC 
CTGCATGTCC CTATCACTCG GAGCGGAGAC CTGAGCTATG AGTCATCAGT GAGGTGCTAT 
ACTCAGAGCC ATTCCGCTCA GGTCATGGAG GACTTTGAGG AGAGACAAAA TGCAGACTCT 
TCACGGATTA CATTTCTCAA AGGGGACAAA GTGAAGAACT GTACGGTCTA TATCCACGAT 
GACTCCATGT TTGAGCCAGA GGAACAGTTC AGGGTCTACC TCGGCCTTCC TCTTGGAAAC 
CACTGGAGTG GAGCTAGAAT TGGAAAGAAT AACATGGCCA CCATCACCAT ATCCAATGAT 
GAAGATGCCC CCACCATTGA GTTTGAAGAA GCTGCATACC AAGTCCGGGA ACCCGCAGGC 
CCAGATGCCA TTGCGATTCT GAACATCAAG GTGATCCGCA GAGGGGATCA GAACAGGACC 
TCCAAGGTTC GCTGCAGCAC GCGGGATGGC TCTGCCCAGT CTGGTGTGGA TTATTACCCA 
AAGAGCCGAG TCTTGAAGTT CAGTCCCGGT GTGGATCATA TCTTTTTTAA AGTTGAGATC 
CTGTCCAATG AAGACCGGGA ATGGCATGAA TCTTTCTCAC TAGTCCTTGG CCCAGATGAC 
CCAGTGGAAG CAGTTCTTGG GGATGTGACT ACTGCCACGG TGACAATTCT AGACCAGGAG 
GCAGCAGGGA GCCTCATATT GCCAGCACCA CCCATTGTGG TCACACTTGC TGACTATGAC 
CATGTGGAAG AAGTTACCAA GGAAGGAGTC AAGAAATCCC CCTCCCCAGG CTACCCACTG 
GTCTGTGTCA CCCCCTGCGA CCCTCATTTC CCCAGATACG CTGTCATGAA GGAGCGCTGC 
AGTGAGGCCG GCATCAACCA GACATCTGTG CAGTTCAGCT GGGAAGTGGC TGCCCCCACT 
GATGGCAATG GGGCCCGGTC TCCCTTTGAA ACCATCACTG ACAACACACC ATTCACCAGT 
GTCAACCACA TGGTCCTGGA CAGCATTTAC TTCAGCCGGA GGTTCCATGT GCGTTGTGTG 
GCCAAGGCTG TGGACAAGGT GGGCCATGTG GGGACCCCCT TAAGGAGCAA CATTGTTACC 
ATTGGAACAG ACAGTGCTAT CTGCCACACA CCAGTGGTGG CTGGGACATC CAGAGGCTTC 
CAGGCTCAGT CCTTCATCGC AACCTTGAAA TACCTGGATG TCAAACATAA GGAGCATCCG 
AACAGAATCC ACATTTCGGT GCAGATCCCA CACCAGGATG GAATGCTGCC CCTTATCTCC 
ACCATGCCGT TGCACAACTT ACATTTTCTA CTGTCTGAGT CCATCTACAG ACACCAGCAC 
GTCTGCTCCA ATTTAGTTAC CACCTATGAC CTGAGAGGCA TCTCAGAGGC AGGGTTCCTG 
GATGATGTGG TCTATGATAG CACTGCCCTG GGGCCTGGCT ACGATCGCCC CTTCCAGTTT 
GACCCCAGCG TGCGAGAGCC GAAGACCATC CAGCTCTACA AACACCTGAA CCTGAAGAGC 
TGCGTGTGGA CCTTTGATGC TTATTATGAC ATGACTGAGC TGATTGACGT CTGTGGGGGC 
TCTGTAACCG CTGACTTCCA GGTGAGGGAC TCTGCCCAGT CCTTCTTGAC AGTGCACGTG 
CCTCTATATG TGTCCTACAT CTATGTGACA GCCCCCAGGG GCTGGGCCTC CTTGGAGCAC 
CACACCGAGA TGGAGTTTTC TTTCTTCTAT GACACTGTTC TCTGGAGAAC AGGAATCCAG 
ACAGACAGCG TGCTCTCTGC AAGGCTTCAG ATAATAAGAA TCTACATTCG AGAGGATGGC 
CGTCTTGTCA TTGAATTCAA GACCCATGCC AAATTCAGAG GACAGTTTGT GATGGAGCAT 
CACACTCTCC CAGAAGTGAA ATCTTTCGTA TTGACTCCAG ACCACCTAGG AGGAATTGAA 
TTTGACTTGC AGCTATTATG GAGCGCTCAG ACTTTTGATT CTCCACATCA ACTCTGGAGA 
GCCACAAGCT CTTATAACAG GAAGGACTAC TCAGGAGAGT ACACCATCTA CCTGATCCCT 
TGCACAGTGC AGCCCACACA GCCATGGGTT GACCCAGGAG AGAAGCCTTT GGCCTGCACT 
GCACATGCCC CAGAAAGATT CCTGATACCC ATTGCATTCC AGCAGACCAA CCGCCCTGTG 
CCAGTTGTGT ATTCACTTAA CACTGAATTT CAGCTCTGCA ATAATGAGAA GGTGTTCCTA 
ATGGATCCCA ATACATCTGA TATGTCACTA GCAGAAATGG ATTACAAAGG AGCCTTTTCA 
AAAGGTCAAA TCCTTTATGG CCGAGTACTT TGGAATCCAG AACAAAATCT TAATTCTGCT 
TACAAACTCC AGCTGGAGAA AGTCTATCTT TGTACGGGCA AGGATGGTTA TGTGCCTTTC 
TTTGATCCCA CGGGGACAAT CTACAATGAA GGGCCCCAGT ATGGATGCAT TCAGCCAAAC 
AAACACCTAA AACACAGATT CCTGCTGTTG GACCGCAATC AGCCAGAGGT AACTGATAAG 
TACTTCCATG ATGTGCCTTT TGAGGCTCAC TTTGCCTCTG AGTTGCCTGA TTTCCATGTG 
GTCAGTAACA TGCCAGGTGT GGATGGATTT ACTCTAAAAG TAGATGCACT CTATAAGGTG 
GAAGCAGGAC ACCAGTGGTA TCTCCAGGTC ATCTACATCA TTGGCCCTGA CACCATCTCA 
GGGCCCCGGG TCCAGCGCTC TCTCACAGCT CCACTCAGAC GCAACCGAAG GGACCTGGTA 
GAGCCCGATG GCCAGCTGAT CCTTGATGAT TCCCTCATCT ATGACAATGA AGGAGACCAA 
GTCAAGAATG GCACCAATAT GAAGTCCCTG AATCTGGAGA TGCAAGAGTT GGCGGTAGCT 
GCGTCCCTGT CACAGACTGG GGCGTCCATT GGCAGTGCCC TGGCTGCAAT CATGCTTCTA 
CTTCTGGTGT TTTTGGTGGC TTGTTTTATC AACAGGAAAT GCCAGAAACA GAGGAAGAAG 
AAGCCCGCAG AGGACATTTT GGAAGAATAT CCTCTGAATA CCAAGGTAGA AGTGCCCAAG 
AGGCACCCGG ACCGGGTGGA GAAGAACGTG AATAGACACT ACTGCACTGT GCGGAACGTC 
AACATCCTGA GTGAGCCTGA GGCGGCTTAC ACGTTCAAAG GTGCTAAAGT CAAAAGACTG 
AATCTAGAAG TCAGAGTTCA CAACAATTTA CAAGATGGAA CAGAAGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714379.1
CDS	688..12498
Translation	MGVLKVWLGLALALAEFAVLPHHSEGACVYQDSLLADATIWKPDSCQSCRCHGDIVICKPAVCRNPQCAFEKGEVLQIAANQCCPECVLRTPGSCHHEKKIHEHGTEWASSPCSVCSCNHGEVRCTPQPCPPLSCGHQELAFIPEGSCCPVCVGLGKPCSYEGHVFQDGEDWRLSRCAKCLCRNGVAQCFTAQCQPLFCNQDETVVRVPGKCCPQCSARSCSAAGQVYEHGEQWSENACTTCICDRGEVRCHKQACLPLRCGKGQSRARRHGQCCEECVSPAGSCSYDGVVRYQDEMWKGSACEFCMCDHGQVTCQTGECAKVECARDEELIHLDGKCCPECISRNGYCVYEETGEFMSSNASEVKRIPEGEKWEDGPCKVCECRGAQVTCYEPSCPPCPVGTLALEVKGQCCPDCTSVHCHPDCLTCSQSPDHCDLCQDPTKLLQNGWCVHSCGLGFYQAGSLCLACQPQCSTCTSGLECSSCQPPLLMRHGQCVPTCGDGFYQDRHSCAVCHESCAGCWGPTEKHCLACRDPLHVLRDGGCESSCGKGFYNRQGTCSACDQSCDSCGPSSPRCLTCTEKTVLHDGKCMSECPGGYYADATGRCKVCHNSCASCSGPTPSHCTACSPPKALRQGHCLPRCGEGFYSDHGVCKACHSSCLACMGPAPSHCTGCKKPEEGLQVEQLSDVGIPSGECLAQCRAHFYLESTGICEACHQSCFRCAGKSPHNCTDCGPSHVLLDGQCLSQCPDGYFHQEGSCTECHPTCRQCHGPLESDCISCYPHISLTNGNCRTSCREEQFLNLVGYCADCHHLCQHCAADLHNTGSICLRCQNAHYLLLGDHCVPDCPSGYYAERGACKKCHSSCRTCQGRGPFSCSSCDTNLVLSHTGTCSTTCFPGHYLDDNHVCQPCNTHCGSCDSQASCTSCRDPNKVLLFGECQYESCAPQYYLDFSTNTCKECDWSCSACSGPLKTDCLQCMDGYVLQDGACVEQCLSSFYQDSGLCKNCDSYCLQCQGPHECTRCKGPFLLLEAQCVQECGKGYFADHAKHKCTACPQGCLQCSHRDRCHLCDHGFFLKSGLCVYNCVPGFSVHTSNETCSGKIHTPSLHVNGSLILPIGSIKPLDFSLLNVQDQEGRVEDLLFHVVSTPTNGQLVLSRNGKEVQLDKAGRFSWKDVNEKKVRFVHSKEKLRKGYLFLKISDQQFFSEPQLINIQAFSTQAPYVLRNEVLHISRGERATITTQMLDIRDDDNPQDVVIEIIDPPLHGQLLQTLQSPATPIYQFQLDELSRGLLHYAHDGSDSTSDVAVLQANDGHSFHNILFQVKTVPQNDRGLQLVANSMVWVPEGGMLQITNRILQAEAPGASAEEIIYKITQDYPQFGEVVLLVNMPADSPADEGQHLPDGRTATPTSTFTQQDINEGIVWYRHSGAPAQSDSFRFEVSSASNAQTRLESHMFNIAILPQTPEAPKVSLEASLHMTAREDGLTVIQPHSLSFINSEKPSGKIVYNITLPLHPNQGIIEHRDHPHSPIRYFTQEDINQGKVMYRPPPAAPHLQELMAFSFAGLPESVKFHFTVSDGEHTSPEMVLTIHLLPSDQQLPVFQVTAPRLAVSPGGSTSVGLQVVVRDAETAPKELFFELRRPPQHGVLLKHTAEFRRPMATGDTFTYEDVEKNALQYIHDGSSTREDSMEISVTDGLTVTMLEVRVEVSLSEDRGPRLAAGSSLSITVASKSTAIITRSHLAYVDDSSPDPEIWIQLNYLPSYGTLLRISGSEVEELSEVSNFTMEDINNKKIRYSAVFETDGHLVTDSFYFSVSDMDHNHLDNQIFTIMITPAENPPPVIAFADLITVDEGGRAPLSFHHFFATDDDDNLQRDAIIKLSALPKYGCIENTGTGDRFGPETASDLEASFPIQDVLENYIYYFQSVHESIEPTHDIFSFYVSDGTSRSEIHSINITIERKNDEPPRMTLQPLRVQLSSGVVISNSSLSLQDLDTPDNELIFVLTKKPDHGHVLWRQTASEPLENGRVLVQGSTFTYQDILAGLVGYVPSVPGMVVDEFQFSLTDGLHVDTGRMKIYTELPASDTPHLAINQGLQLSAGSVARITEQHLKVTDIDSDDHQVMYIMKEDPGAGRLQMMKHGNLEQISIKGPIRSFTQADISQGHVEYSHGTGEPGGSFAFKFDVVDGEGNRLIDKSFSISILEDKSPPVITTNKGLVLDENSVKKITTLQLSATDQDSGPTELIYRITRQPQLGHLEHAASPGIQISSFTQADLTSRNVQYVHSSEAEKHSDAFSFTLSDGVSEVTQTFHITLHPVDDSLPVVQNLGMRVQEGMRKTITEFELKAVDADTEIMTAAPQPFRVDILPVDDGTPRIVTNLGLQWLEYMDGKATNLITKKELLTMDPDTEDAQLVYEITTGPKHGFVENKLQPGRAAATFTQEDVNLGLIRYVLHKEKIREMMDSFQFLVKDSKPNVVSDNVFHIQWSLISFKYTSYNVSEKAGSVSVTVQRTGNLNQYAIVLCRTEQGTASSSSRVSSQPGQQDYVEYAGQVQFDEREDTKSCTIVINDDDVFENVESFTVELSMPAYALLGEFTQAKVIINDTEDEPTLEFDKKIYWVNESAGFLFAPIERKGDASSIVSAICYTVPKSAMGSSLYALESGSDFKSRGMSAASRVIFGPGVTMSTCDVMLIDDSEYEEEEEFEIALADASDNARIGRVATAKVLISGPNDASTVSLGNTAFTVSEDAGTVKIPVIRHGTDLSTFASVWCATRPSDPASATPGVDYVPSSRKVEFGPGVIEQYCTLTILDDTQYPVIEGLETFVVFLSSAQGAELTKPFQAVIAINDTFQDVPSMQFAKDLLLVKEKEGVLHVPITRSGDLSYESSVRCYTQSHSAQVMEDFEERQNADSSRITFLKGDKVKNCTVYIHDDSMFEPEEQFRVYLGLPLGNHWSGARIGKNNMATITISNDEDAPTIEFEEAAYQVREPAGPDAIAILNIKVIRRGDQNRTSKVRCSTRDGSAQSGVDYYPKSRVLKFSPGVDHIFFKVEILSNEDREWHESFSLVLGPDDPVEAVLGDVTTATVTILDQEAAGSLILPAPPIVVTLADYDHVEEVTKEGVKKSPSPGYPLVCVTPCDPHFPRYAVMKERCSEAGINQTSVQFSWEVAAPTDGNGARSPFETITDNTPFTSVNHMVLDSIYFSRRFHVRCVAKAVDKVGHVGTPLRSNIVTIGTDSAICHTPVVAGTSRGFQAQSFIATLKYLDVKHKEHPNRIHISVQIPHQDGMLPLISTMPLHNLHFLLSESIYRHQHVCSNLVTTYDLRGISEAGFLDDVVYDSTALGPGYDRPFQFDPSVREPKTIQLYKHLNLKSCVWTFDAYYDMTELIDVCGGSVTADFQVRDSAQSFLTVHVPLYVSYIYVTAPRGWASLEHHTEMEFSFFYDTVLWRTGIQTDSVLSARLQIIRIYIREDGRLVIEFKTHAKFRGQFVMEHHTLPEVKSFVLTPDHLGGIEFDLQLLWSAQTFDSPHQLWRATSSYNRKDYSGEYTIYLIPCTVQPTQPWVDPGEKPLACTAHAPERFLIPIAFQQTNRPVPVVYSLNTEFQLCNNEKVFLMDPNTSDMSLAEMDYKGAFSKGQILYGRVLWNPEQNLNSAYKLQLEKVYLCTGKDGYVPFFDPTGTIYNEGPQYGCIQPNKHLKHRFLLLDRNQPEVTDKYFHDVPFEAHFASELPDFHVVSNMPGVDGFTLKVDALYKVEAGHQWYLQVIYIIGPDTISGPRVQRSLTAPLRRNRRDLVEPDGQLILDDSLIYDNEGDQVKNGTNMKSLNLEMQELAVAASLSQTGASIGSALAAIMLLLLVFLVACFINRKCQKQRKKKPAEDILEEYPLNTKVEVPKRHPDRVEKNVNRHYCTVRNVNILSEPEAAYTFKGAKVKRLNLEVRVHNNLQDGTEV

Target ORF information:

RefSeq Version	XM_006714316.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Fraser extracellular matrix complex subunit 1 (FRAS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714316.2

ORF Insert Sequence:

ATGGGTGTCC TCAAAGTGTG GCTCGGGCTG GCCCTAGCGT TGGCGGAATT TGCAGTATTG 
CCTCATCATT CCGAAGGTGC TTGTGTCTAT CAGGATTCCT TGTTGGCGGA TGCCACAATT 
TGGAAGCCCG ATTCATGCCA GAGCTGCCGT TGCCATGGTG ATATTGTTAT CTGCAAACCT 
GCTGTTTGCA GAAACCCTCA ATGTGCCTTT GAGAAGGGAG AAGTGCTTCA AATAGCTGCC 
AACCAATGCT GTCCTGAGTG TGTTTTGAGG ACTCCAGGAT CTTGCCATCA TGAAAAGAAA 
ATCCATGAGC ATGGGACAGA ATGGGCCTCT TCTCCATGTA GTGTGTGCTC TTGCAATCAT 
GGGGAAGTCC GATGTACCCC CCAACCATGC CCACCGCTGT CATGTGGACA CCAGGAGCTG 
GCATTCATCC CTGAAGGAAG CTGCTGCCCA GTTTGTGTGG GCCTTGGGAA ACCCTGTTCC 
TATGAAGGCC ATGTGTTTCA GGATGGGGAG GACTGGCGGC TGAGCCGGTG TGCCAAATGT 
CTGTGTAGAA ATGGGGTTGC CCAGTGCTTC ACAGCTCAGT GTCAGCCTCT ATTTTGTAAC 
CAGGATGAGA CTGTAGTCCG AGTCCCTGGA AAATGTTGCC CGCAGTGCTC TGCAAGATCC 
TGCTCTGCAG CTGGCCAAGT ATACGAGCAT GGTGAGCAGT GGAGCGAAAA TGCCTGCACC 
ACGTGTATAT GTGACCGGGG TGAGGTCAGG TGTCACAAGC AGGCCTGCCT GCCCCTGAGA 
TGCGGAAAGG GTCAGAGCAG GGCTCGGCGT CATGGGCAAT GCTGTGAGGA ATGTGTGTCT 
CCTGCCGGGA GCTGCTCCTA TGATGGAGTT GTGCGGTACC AGGACGAAAT GTGGAAGGGC 
TCGGCCTGTG AGTTCTGCAT GTGTGATCAT GGCCAAGTGA CCTGCCAGAC TGGAGAGTGT 
GCCAAAGTGG AGTGTGCCCG GGATGAAGAA TTAATTCACT TAGATGGAAA GTGTTGTCCT 
GAATGCATTT CAAGGAATGG TTATTGTGTT TATGAAGAAA CTGGAGAATT TATGTCATCA 
AATGCTAGTG AAGTTAAACG TATTCCAGAG GGAGAGAAGT GGGAAGATGG CCCTTGCAAG 
GTGTGTGAGT GCCGAGGGGC TCAGGTAACT TGCTACGAGC CCTCTTGCCC ACCATGTCCA 
GTGGGCACAC TGGCCTTAGA GGTGAAGGGA CAGTGCTGTC CAGACTGCAC ATCAGTTCAT 
TGCCATCCAG ATTGTTTGAC ATGCTCTCAG TCTCCAGACC ACTGTGACCT CTGCCAAGAT 
CCTACCAAGT TACTGCAGAA TGGATGGTGT GTGCACAGCT GTGGACTGGG TTTTTACCAA 
GCTGGCAGTC TCTGTTTAGC CTGCCAGCCC CAGTGCTCCA CGTGTACCAG TGGGCTGGAG 
TGCTCATCCT GCCAGCCTCC CCTGCTGATG CGGCACGGGC AGTGTGTGCC TACCTGTGGG 
GACGGCTTCT ACCAAGATCG CCATTCCTGT GCAGTCTGCC ATGAGTCCTG TGCAGGTTGC 
TGGGGCCCAA CGGAGAAGCA CTGCTTGGCC TGCAGAGATC CCCTCCACGT GCTGAGAGAT 
GGCGGCTGTG AGAGCAGCTG TGGAAAAGGC TTCTACAACA GGCAGGGCAC CTGTAGCGCT 
TGTGACCAAT CCTGTGACAG TTGTGGCCCC AGTAGCCCCA GGTGTCTTAC CTGTACTGAG 
AAGACAGTGC TGCATGATGG GAAATGCATG TCTGAATGCC CTGGCGGGTA CTATGCTGAT 
GCCACTGGCA GGTGCAAAGT TTGTCATAAC TCATGTGCCA GCTGCTCTGG GCCCACACCC 
TCTCACTGTA CAGCCTGCAG CCCCCCCAAG GCTCTGCGTC AAGGCCACTG TCTGCCCCGC 
TGTGGAGAGG GTTTCTACTC TGACCATGGA GTCTGCAAAG CCTGTCACTC CTCCTGCCTG 
GCTTGTATGG GTCCCGCACC CTCTCACTGT ACTGGGTGTA AGAAGCCAGA GGAAGGACTG 
CAAGTGGAGC AGCTGTCTGA CGTGGGCATC CCCTCTGGCG AGTGTCTAGC CCAGTGTAGA 
GCCCATTTTT ACTTGGAGAG CACTGGCATA TGTGAAGCTT GCCACCAGTC CTGTTTCAGA 
TGTGCAGGGA AAAGCCCACA TAACTGCACA GACTGTGGGC CTTCCCATGT GCTGTTGGAT 
GGGCAGTGCC TCTCCCAGTG CCCAGATGGC TACTTTCACC AGGAAGGTAG TTGCACAGAG 
TGTCACCCAA CCTGCAGGCA GTGTCATGGG CCGTTGGAGT CTGACTGCAT CTCCTGTTAC 
CCTCACATCT CTCTTACCAA TGGTAACTGC AGGACCAGCT GCAGGGAAGA GCAGTTCCTC 
AACCTCGTGG GATACTGTGC TGACTGCCAT CACCTGTGCC AGCACTGTGC AGCTGATCTC 
CACAACACTG GGAGCATCTG CCTCAGGTGC CAGAATGCCC ACTACCTGCT GCTCGGGGAC 
CACTGTGTTC CTGACTGCCC TTCAGGATAC TATGCAGAGA GAGGAGCTTG TAAAAAATGC 
CACTCCTCCT GCAGAACCTG CCAGGGCAGA GGACCTTTCT CCTGCTCCTC ATGTGACACC 
AACCTCGTGC TGTCCCACAC TGGCACCTGC AGCACCACCT GCTTCCCTGG GCACTATCTT 
GATGACAATC ATGTTTGCCA GCCATGCAAC ACACACTGTG GAAGCTGTGA TTCACAGGCC 
AGCTGTACCT CCTGCCGAGA TCCAAACAAG GTTCTGCTCT TTGGGGAATG TCAATACGAG 
AGCTGCGCCC CACAGTACTA TCTTGACTTC TCCACCAACA CGTGCAAAGA GTGTGATTGG 
AGCTGCAGTG CATGCAGTGG GCCCCTGAAA ACAGACTGCC TGCAGTGCAT GGATGGCTAT 
GTTCTCCAGG ATGGGGCCTG CGTGGAGCAG TGCTTGTCAT CATTTTACCA GGACTCGGGC 
CTCTGCAAGA ACTGTGACAG CTACTGTCTC CAGTGCCAAG GTCCCCATGA GTGTACCCGC 
TGCAAAGGGC CATTTCTCCT CTTGGAAGCC CAGTGTGTCC AGGAATGTGG GAAGGGGTAC 
TTTGCAGATC ATGCAAAGCA CAAATGCACA GCCTGCCCTC AGGGGTGCTT GCAGTGCAGC 
CACAGGGACC GTTGTCACCT CTGTGACCAT GGGTTCTTTC TGAAGAGTGG CCTCTGTGTT 
TACAACTGTG TTCCTGGCTT TTCTGTCCAC ACCTCTAATG AAACATGTTC TGGCAAAATA 
CACACCCCTA GTCTTCATGT GAATGGTTCC CTGATCCTCC CAATTGGTTC AATAAAGCCA 
CTGGATTTTT CCCTCCTGAA TGTCCAAGAC CAGGAGGGTA GGGTCGAAGA TCTCCTATTT 
CATGTTGTGA GCACTCCCAC CAATGGTCAG CTAGTGCTCT CAAGAAATGG AAAAGAGGTT 
CAGCTGGACA AGGCTGGCCG TTTTAGCTGG AAAGATGTGA ACGAGAAGAA AGTGCGTTTT 
GTGCACAGCA AAGAAAAACT CAGGAAAGGT TACCTTTTCC TGAAAATTAG TGACCAGCAG 
TTCTTCTCTG AGCCACAGCT GATCAACATA CAAGCATTTT CAACACAGGC CCCCTATGTG 
CTGAGAAATG AAGTTCTCCA CATTAGCAGA GGAGAGAGGG CAACCATCAC CACCCAGATG 
CTTGACATCC GAGATGATGA CAACCCACAG GATGTGGTCA TTGAAATAAT CGATCCTCCA 
CTTCATGGCC AATTGCTTCA GACACTTCAG TCCCCGGCAA CCCCTATCTA TCAATTCCAG 
CTGGATGAAC TCTCTAGAGG CCTTCTCCAC TATGCTCATG ATGGTTCAGA CAGCACATCC 
GATGTTGCAG TCTTGCAGGC CAATGATGGA CACTCCTTCC ATAATATACT GTTCCAAGTG 
AAGACCGTGC CTCAGAATGA CAGGGGTCTT CAGCTTGTGG CTAATTCGAT GGTGTGGGTT 
CCAGAAGGGG GGATGCTGCA GATCACCAAC AGAATCTTAC AGGCCGAGGC TCCTGGTGCC 
AGTGCTGAAG AAATCATCTA CAAGATTACA CAAGACTACC CCCAGTTTGG GGAGGTGGTC 
CTTCTAGTGA ATATGCCTGC AGACAGCCCT GCAGATGAAG GGCAGCACCT GCCTGATGGG 
AGGACAGCTA CCCCCACCAG CACCTTCACC CAGCAGGACA TCAATGAAGG CATCGTATGG 
TACAGGCACT CAGGAGCCCC AGCCCAGAGC GACTCCTTCC GCTTCGAGGT GTCCAGTGCC 
TCCAATGCCC AGACCCGCCT GGAGAGCCAC ATGTTCAACA TCGCGATCTT ACCACAGACA 
CCTGAAGCAC CTAAAGTGTC TCTGGAAGCA TCTCTCCATA TGACTGCTAG AGAAGATGGC 
CTGACTGTTA TTCAGCCTCA TTCCCTCTCC TTCATAAACT CTGAGAAGCC AAGTGGAAAG 
ATTGTCTACA ACATCACTCT ACCTCTGCAT CCAAATCAAG GTATCATCGA GCACCGGGAC 
CACCCTCACT CTCCTATCCG GTATTTCACG CAAGAGGATA TTAACCAGGG CAAAGTCATG 
TACCGCCCTC CCCCGGCAGC ACCCCACCTC CAGGAGCTCA TGGCCTTCTC GTTCGCTGGT 
CTCCCAGAAT CAGTGAAATT CCACTTCACA GTTTCAGATG GAGAACACAC AAGTCCGGAG 
ATGGTCCTCA CCATTCACTT ACTTCCCAGT GATCAGCAAC TGCCAGTGTT CCAGGTCACA 
GCTCCACGGC TGGCGGTCAG CCCAGGAGGC AGCACTTCTG TAGGACTTCA GGTGGTAGTA 
AGAGATGCTG AGACAGCGCC CAAAGAACTC TTCTTTGAGC TTCGGAGACC TCCACAGCAT 
GGTGTGCTTC TTAAGCATAC AGCTGAGTTC CGAAGGCCGA TGGCCACAGG TGACACTTTC 
ACCTATGAGG ATGTTGAGAA AAATGCTCTA CAGTATATAC ATGATGGTTC CTCTACCCGG 
GAAGACAGCA TGGAGATCTC AGTCACAGAT GGCCTCACAG TGACAATGCT GGAGGTGAGA 
GTAGAGGTGT CCCTGTCAGA AGACCGAGGG CCTCGACTGG CTGCTGGCTC CTCTCTGAGC 
ATTACTGTTG CCAGTAAAAG CACAGCCATA ATCACTAGGT CACACCTTGC TTACGTGGAT 
GATTCTTCCC CCGACCCAGA GATCTGGATT CAGTTAAATT ATCTGCCCTC ATATGGTACT 
CTCTTAAGAA TCTCAGGATC TGAGGTGGAA GAGCTCTCAG AAGTTTCCAA TTTCACAATG 
GAAGACATCA ATAACAAGAA AATCAGATAC TCAGCTGTGT TTGAAACTGA TGGTCATCTG 
GTTACTGATA GCTTCTATTT CTCTGTCTCT GACATGGACC ACAACCATCT GGATAATCAG 
ATATTTACCA TCATGATCAC TCCTGCTGAA AATCCACCTC CAGTCATTGC TTTTGCTGAC 
CTTATCACGG TTGATGAGGG AGGGAGAGCA CCACTCTCAT TTCACCATTT TTTTGCTACT 
GATGATGATG ACAACCTCCA GAGAGATGCC ATCATTAAAC TAAGTGCTCT GCCCAAATAT 
GGCTGCATTG AGAACACAGG AACAGGTGAT CGTTTTGGCC CTGAAACTGC CAGTGACCTA 
GAGGCATCAT TTCCTATTCA AGACGTCCTG GAAAACTACA TTTACTACTT TCAGAGTGTT 
CATGAAAGCA TTGAGCCAAC CCATGATATT TTTAGTTTTT ATGTGAGTGA TGGAACCAGT 
CGTTCAGAAA TTCACAGCAT CAATATCACC ATTGAGAGGA AGAACGATGA GCCTCCCAGG 
ATGACCTTGC AGCCCCTCAG AGTGCAGCTG AGCTCGGGAG TGGTGATAAG CAATTCTTCT 
TTGAGCCTGC AAGACCTGGA CACCCCAGAT AATGAGCTCA TTTTTGTATT GACAAAAAAG 
CCTGACCACG GTCATGTACT CTGGAGGCAA ACTGCTTCTG AGCCTCTGGA GAATGGGAGA 
GTTTTAGTCC AGGGCTCAAC CTTCACCTAC CAGGATATCC TAGCTGGGCT GGTTGGGTAT 
GTGCCTAGTG TCCCTGGCAT GGTCGTGGAT GAGTTCCAGT TCTCCCTCAC TGATGGCCTC 
CACGTGGACA CAGGGAGGAT GAAGATCTAC ACAGAACTGC CTGCAAGTGA CACACCTCAC 
TTGGCTATAA ACCAAGGCCT ACAGCTCTCA GCAGGGTCTG TAGCACGCAT CACAGAACAG 
CACTTGAAAG TGACAGATAT TGACTCAGAT GACCATCAGG TTATGTACAT CATGAAGGAA 
GATCCTGGTG CAGGGCGCCT GCAGATGATG AAGCATGGCA ACCTGGAGCA AATTTCTATT 
AAAGGCCCCA TCCGAAGTTT CACCCAGGCA GACATTAGCC AAGGCCACGT AGAATATAGT 
CATGGAACAG GAGAACCTGG AGGGAGCTTT GCTTTTAAAT TTGATGTGGT TGATGGAGAA 
GGCAACAGAT TGATTGACAA GTCATTTTCC ATCAGCATTC TAGAAGACAA ATCCCCACCA 
GTCATCACCA CCAATAAAGG ACTGGTCTTG GATGAAAACT CAGTGAAGAA AATCACCACC 
CTGCAGCTGT CTGCCACTGA CCAGGACAGT GGGCCTACAG AATTGATCTA CAGAATCACC 
AGACAGCCCC AGCTGGGCCA CTTGGAACAT GCAGCATCAC CAGGTATCCA GATTAGTTCC 
TTTACTCAAG CTGATCTGAC TTCACGAAAT GTTCAGTATG TCCATTCTAG TGAGGCTGAG 
AAACATTCAG ATGCCTTCAG CTTTACACTG TCTGATGGAG TCAGTGAGGT GACTCAGACT 
TTCCATATCA CTCTTCACCC TGTCGATGAT TCGCTGCCCG TCGTACAGAA CTTAGGAATG 
CGGGTGCAGG AGGGCATGAG GAAGACCATC ACAGAGTTTG AGCTTAAGGC GGTGGATGCT 
GACACAGAGA TTATGACAGC AGCACCTCAG CCGTTCCGAG TAGACATCCT CCCGGTAGAT 
GATGGCACGC CTAGAATTGT CACCAACCTG GGACTCCAGT GGCTGGAATA CATGGATGGC 
AAGGCAACCA ACCTGATCAC CAAGAAGGAA CTGCTGACCA TGGACCCAGA CACCGAGGAC 
GCGCAGCTTG TCTATGAGAT AACGACGGGC CCTAAGCATG GCTTTGTGGA GAACAAGCTG 
CAGCCTGGCA GAGCTGCTGC CACTTTCACC CAGGAGGATG TGAACTTGGG GTTGATTCGT 
TATGTGTTGC ACAAGGAGAA GATCCGTGAG ATGATGGATA GTTTTCAGTT TCTGGTGAAA 
GACAGTAAAC CCAATGTGGT CAGCGACAAT GTCTTCCATA TCCAGTGGTC ACTCATCAGC 
TTTAAATATA CCAGCTACAA TGTCAGTGAG AAGGCAGGGT CTGTCAGTGT CACGGTGCAG 
AGGACTGGGA ACCTGAACCA ATATGCCATC GTCCTGTGTC GCACCGAGCA AGGCACCGCC 
AGCTCCAGCT CCAGGGTCAG CTCCCAACCT GGGCAACAGG ACTATGTAGA GTATGCTGGC 
CAGGTCCAGT TTGATGAGCG AGAGGACACC AAGTCCTGCA CCATTGTCAT CAACGATGAT 
GACGTGTTTG AAAATGTTGA GAGTTTCACT GTGGAGCTCA GCATGCCAGC TTATGCCCTG 
TTAGGGGAAT TCACCCAGGC GAAGGTCATT ATCAACGATA CCGAGGATGA ACCCACATTA 
GAGTTTGACA AGAAGATCTA CTGGGTTAAC GAGAGCGCTG GTTTTCTGTT TGCACCTATT 
GAAAGAAAAG GAGATGCAAG CAGCATTGTA TCTGCAATTT GCTACACAGT CCCTAAGTCA 
GCTATGGGAA GTAGCCTCTA TGCTCTAGAA TCAGGCTCTG ATTTTAAATC TAGAGGGATG 
TCTGCCGCGA GTCGTGTGAT ATTCGGGCCT GGTGTGACCA TGTCCACCTG TGATGTCATG 
CTTATTGATG ACAGCGAGTA TGAAGAGGAA GAAGAGTTTG AGATTGCCTT GGCAGATGCC 
TCTGACAATG CCCGCATTGG AAGGGTGGCG ACAGCCAAGG TGCTCATTAG TGGTCCCAAC 
GATGCCTCGA CTGTGTCCCT GGGCAACACG GCTTTCACTG TCAGTGAGGA CGCAGGCACA 
GTAAAGATTC CAGTTATCCG CCATGGTACT GACCTCTCTA CTTTCGCATC TGTCTGGTGT 
GCAACGCGGC CCTCAGACCC AGCTTCTGCC ACACCAGGAG TTGACTACGT TCCCAGCTCT 
CGGAAGGTGG AATTTGGGCC TGGTGTCATT GAACAGTATT GCACCTTGAC TATCTTGGAT 
GACACTCAGT ATCCGGTAAT TGAAGGACTG GAGACATTTG TGGTTTTCCT CAGCTCAGCA 
CAAGGAGCCG AACTGACCAA ACCCTTCCAG GCAGTCATTG CAATTAATGA CACATTCCAA 
GATGTGCCCA GCATGCAGTT TGCCAAGGAT TTGCTCCTAG TGAAGGAGAA GGAGGGTGTC 
CTGCATGTCC CTATCACTCG GAGCGGAGAC CTGAGCTATG AGTCATCAGT GAGGTGCTAT 
ACTCAGAGCC ATTCCGCTCA GGTCATGGAG GACTTTGAGG AGAGACAAAA TGCAGACTCT 
TCACGGATTA CATTTCTCAA AGGGGACAAA GTGAAGAACT GTACGGTCTA TATCCACGAT 
GACTCCATGT TTGAGCCAGA GGAACAGTTC AGGGTCTACC TCGGCCTTCC TCTTGGAAAC 
CACTGGAGTG GAGCTAGAAT TGGAAAGAAT AACATGGCCA CCATCACCAT ATCCAATGAT 
GAAGATGCCC CCACCATTGA GTTTGAAGAA GCTGCATACC AAGTCCGGGA ACCCGCAGGC 
CCAGATGCCA TTGCGATTCT GAACATCAAG GTGATCCGCA GAGGGGATCA GAACAGGACC 
TCCAAGGTTC GCTGCAGCAC GCGGGATGGC TCTGCCCAGT CTGGTGTGGA TTATTACCCA 
AAGAGCCGAG TCTTGAAGTT CAGTCCCGGT GTGGATCATA TCTTTTTTAA AGTTGAGATC 
CTGTCCAATG AAGACCGGGA ATGGCATGAA TCTTTCTCAC TAGTCCTTGG CCCAGATGAC 
CCAGTGGAAG CAGTTCTTGG GGATGTGACT ACTGCCACGG TGACAATTCT AGACCAGGAG 
GCAGCAGGGA GCCTCATATT GCCAGCACCA CCCATTGTGG TCACACTTGC TGACTATGAC 
CATGTGGAAG AAGTTACCAA GGAAGGAGTC AAGAAATCCC CCTCCCCAGG CTACCCACTG 
GTCTGTGTCA CCCCCTGCGA CCCTCATTTC CCCAGATACG CTGTCATGAA GGAGCGCTGC 
AGTGAGGCCG GCATCAACCA GACATCTGTG CAGTTCAGCT GGGAAGTGGC TGCCCCCACT 
GATGGCAATG GGGCCCGGTC TCCCTTTGAA ACCATCACTG ACAACACACC ATTCACCAGT 
GTCAACCACA TGGTCCTGGA CAGCATTTAC TTCAGCCGGA GGTTCCATGT GCGTTGTGTG 
GCCAAGGCTG TGGACAAGGT GGGCCATGTG GGGACCCCCT TAAGGAGCAA CATTGTTACC 
ATTGGAACAG ACAGTGCTAT CTGCCACACA CCAGTGGTGG CTGGGACATC CAGAGGCTTC 
CAGGCTCAGT CCTTCATCGC AACCTTGAAA TACCTGGATG TCAAACATAA GGAGCATCCG 
AACAGAATCC ACATTTCGGT GCAGATCCCA CACCAGGATG GAATGCTGCC CCTTATCTCC 
ACCATGCCGT TGCACAACTT ACATTTTCTA CTGTCTGAGT CCATCTACAG ACACCAGCAC 
GTCTGCTCCA ATTTAGTTAC CACCTATGAC CTGAGAGGCA TCTCAGAGGC AGGGTTCCTG 
GATGATGTGG TCTATGATAG CACTGCCCTG GGGCCTGGCT ACGATCGCCC CTTCCAGTTT 
GACCCCAGCG TGCGAGAGCC GAAGACCATC CAGCTCTACA AACACCTGAA CCTGAAGAGC 
TGCGTGTGGA CCTTTGATGC TTATTATGAC ATGACTGAGC TGATTGACGT CTGTGGGGGC 
TCTGTAACCG CTGACTTCCA GGTGAGGGAC TCTGCCCAGT CCTTCTTGAC AGTGCACGTG 
CCTCTATATG TGTCCTACAT CTATGTGACA GCCCCCAGGG GCTGGGCCTC CTTGGAGCAC 
CACACCGAGA TGGAGTTTTC TTTCTTCTAT GACACTGTTC TCTGGAGAAC AGGAATCCAG 
ACAGACAGCG TGCTCTCTGC AAGGCTTCAG ATAATAAGAA TCTACATTCG AGAGGATGGC 
CGTCTTGTCA TTGAATTCAA GACCCATGCC AAATTCAGAG GACAGTTTGT GATGGAGCAT 
CACACTCTCC CAGAAGTGAA ATCTTTCGTA TTGACTCCAG ACCACCTAGG AGGAATTGAA 
TTTGACTTGC AGCTATTATG GAGCGCTCAG ACTTTTGATT CTCCACATCA ACTCTGGAGA 
GCCACAAGCT CTTATAACAG GAAGGACTAC TCAGGAGAGT ACACCATCTA CCTGATCCCT 
TGCACAGTGC AGCCCACACA GCCATGGGTT GACCCAGGAG AGAAGCCTTT GGCCTGCACT 
GCACATGCCC CAGAAAGATT CCTGATACCC ATTGCATTCC AGCAGACCAA CCGCCCTGTG 
CCAGTTGTGT ATTCACTTAA CACTGAATTT CAGCTCTGCA ATAATGAGAA GGTGTTCCTA 
ATGGATCCCA ATACATCTGA TATGTCACTA GCAGAAATGG ATTACAAAGG AGCCTTTTCA 
AAAGGTCAAA TCCTTTATGG CCGAGTACTT TGGAATCCAG AACAAAATCT TAATTCTGCT 
TACAAACTCC AGCTGGAGAA AGTCTATCTT TGTACGGGCA AGGATGGTTA TGTGCCTTTC 
TTTGATCCCA CGGGGACAAT CTACAATGAA GGGCCCCAGT ATGGATGCAT TCAGCCAAAC 
AAACACCTAA AACACAGATT CCTGCTGTTG GACCGCAATC AGCCAGAGGT AACTGATAAG 
TACTTCCATG ATGTGCCTTT TGAGGCTCAC TTTGCCTCTG AGTTGCCTGA TTTCCATGTG 
GTCAGTAACA TGCCAGGTGT GGATGGATTT ACTCTAAAAG TAGATGCACT CTATAAGGTG 
GAAGCAGGAC ACCAGTGGTA TCTCCAGGTC ATCTACATCA TTGGCCCTGA CACCATCTCA 
GGGCCCCGGG TCCAGCGCTC TCTCACAGCT CCACTCAGAC GCAACCGAAG GGACCTGGTA 
GAGCCCGATG GCCAGCTGAT CCTTGATGAT TCCCTCATCT ATGACAATGA AGGAGACCAA 
GTCAAGAATG GCACCAATAT GAAGTCCCTG AATCTGGAGA TGCAAGAGTT GGCGGTAGCT 
GCGTCCCTGT CACAGACTGG GGCGTCCATT GGCAGTGCCC TGGCTGCAAT CATGCTTCTA 
CTTCTGGTGT TTTTGGTGGC TTGTTTTATC AACAGGAAAT GCCAGAAACA GAGGAAGAAG 
AAGCCCGCAG AGGACATTTT GGAAGAATAT CCTCTGAATA CCAAGGTAGA AGTGCCCAAG 
AGGCACCCGG ACCGGGTGGA GAAGAACGTG AATAGACACT ACTGCACTGT GCGGAACGTC 
AACATCCTGA GTGAGCCTGA GGCGGCTTAC ACGTTCAAAG GTGCTAAAGT CAAAAGACTG 
AATCTAGAAG TCAGAGTTCA CAACAATTTA CAAGATGGAA CAGAAGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mild recessive mutations in six Fraser syndrome-related genes cause isolated congenital anomalies of the kidney and urinary tract.
Journal of the American Society of Nephrology : JASN25(9)1917-22(2014 Sep)
Kohl S,Hwang DY,Dworschak GC,Hilger AC,Saisawat P,Vivante A,Stajic N,Bogdanovic R,Reutter HM,Kehinde EO,Tasic V,Hildebrandt F

A genome wide association study of plasma uric acid levels in obese cases and never-overweight controls.
Obesity (Silver Spring, Md.)21(9)E490-4(2013 Sep)
Li WD,Jiao H,Wang K,Zhang CK,Glessner JT,Grant SF,Zhao H,Hakonarson H,Arlen Price R

Expanding the mutation spectrum for Fraser syndrome: identification of a novel heterozygous deletion in FRAS1.
Gene520(2)194-7(2013 May)
Hoefele J,Wilhelm C,Schiesser M,Mack R,Heinrich U,Weber LT,Biskup S,Daumer-Haas C,Klein HG,Rost I

A puzzle over several decades: eye anomalies with FRAS1 and STRA6 mutations in the same family.
Clinical genetics83(2)162-8(2013 Feb)
Ng WY,Pasutto F,Bardakjian TM,Wilson MJ,Watson G,Schneider A,Mackey DA,Grigg JR,Zenker M,Jamieson RV

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FRAS1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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