PIEZO2 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017025918.1	XP_016881407.1	piezo-type mechanosensitive ion channel component 2 isoform X2
XM_011525724.3	XP_011524026.1	piezo-type mechanosensitive ion channel component 2 isoform X3
XM_011525726.3	XP_011524028.1	piezo-type mechanosensitive ion channel component 2 isoform X5
XM_011525723.2	XP_011524025.1	piezo-type mechanosensitive ion channel component 2 isoform X1
XM_011525726.2	XP_011524028.1	piezo-type mechanosensitive ion channel component 2 isoform X5
XM_011525725.3	XP_011524027.1	piezo-type mechanosensitive ion channel component 2 isoform X4
XM_011525723.3	XP_011524025.1	piezo-type mechanosensitive ion channel component 2 isoform X1
XM_017025918.2	XP_016881407.1	piezo-type mechanosensitive ion channel component 2 isoform X2
NM_022068.3	NP_071351.2	piezo-type mechanosensitive ion channel component 2
XM_011525724.2	XP_011524026.1	piezo-type mechanosensitive ion channel component 2 isoform X3
XM_011525725.2	XP_011524027.1	piezo-type mechanosensitive ion channel component 2 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28481	NM_022068.3 Latest version!	Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu71131	XM_011525723.3 Latest version!	Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu71132	XM_011525724.3 Latest version!	Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu71133	XM_011525725.3 Latest version!	Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu71134	XM_011525726.3 Latest version!	Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu88005	XM_017025918.2 Latest version!	Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OHu71134	XM_011525726.2	Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu71131	XM_011525723.2	Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu88005	XM_017025918.1	Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu71132	XM_011525724.2	Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu71133	XM_011525725.2	Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

PIEZO2 ( NM_022068.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_071351.2 Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), mRNA.

Price & Availability↑

CloneID	OHu28481
Clone ID Related Accession (Same CDS sequence)	NM_022068.3
Accession Version	NM_022068.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	piezo-type mechanosensitive ion channel component 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP005404.2, AP005120.2, AP005117.2, AP001180.4 and BM974712.1. This sequence is a reference standard in the RefSeqGene project. On Mar 6, 2014 this sequence version replaced NM_022068.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. There are no full-length transcripts representing this gene; this RefSeq is predicted based on a combination of partial human and mouse transcript alignments, but it is unclear if this exon combination exists in vivo. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: JN790819.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCAG AAGTGGTGTG CGGGCTCATC TTCAGGCTGC TGCTGCCCAT CTGCCTGGCA 
GTAGCATGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC 
ATTCCTCTGT TCTCAGAACC AACAAAAACG ACGATGCAAG GACATACGGG ACGGTTATTA 
AAGTCTCTGT GCTTCATCAG TCTTTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG 
TTGGTGAGCC TTGAAGCTCA ACATCGTATT GCACCTGGCT ACAACTGCTC AACATGGGAA 
AAGACATTCC GGCAGATCGG CTTTGAAAGC TTAAAGGGAG CTGATGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCCTCTGT 
AGAAACATTG TTCAGAAACC TGTGACAGAC GAAGCAGCAC AGAGTAACCC GGAGTTTGAA 
AATGAAGAAT TGGCTGAAGG AGAAAAAATT GATTCAGAAG AGGCACTGAT CTATGAAGAG 
GATTTCAATG GAGGAGATGG TGTTGAAGGC GAGTTGGAAG AAAGCACGAA GTTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCCTCTAAG CTCAAGGAGT TCATTGGCAA CATGATCACC 
ACTGCTGGGA AAGTCGTTGT TACCATCTTA CTGGGCTCCT CGGGCATGAT GTTGCCGTCT 
TTGACATCAT CTGTGTATTT TTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACTGCT 
GGACATTTGA TTGGACTTTA TTTATACCAG TTCCAATTCT TTCAAGAGGC AGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAGTCAGTAA TTCAAACGGA CTGTTCAAGT 
ACTTGGAAGA TCATAGTGAA CCCGGACCTG TCGTGGTACC ACCACGCCAA CCCTATCCTC 
CTGCTGGTGA TGTACTACAC TCTGGCCACT CTGATCCGCA TCTGGCTGCA AGAGCCCCTT 
GTGCAGGATG AGGGGACCAA AGAAGAGGAC AAAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACAGCGGGGA GGAGGCGGAG CCTGTGGTAC GCAACCCATT ACCCCACTGA TGAGAGAAAA 
CTTTTATCCA TGACCCAGGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC 
GGCAACCCCG TGGATTACCA CACCATCCAC CCAAGCCTGC CCATGGAGAA CGGCCCTGGC 
AAAGCCGACC TCTACTCCAC CCCTCAGTAC CGGTGGGAGC CCTCTGATGA ATCCTCAGAA 
AAGCGAGAGG AGGAAGAGGA AGAGAAAGAA GAATTTGAAG AAGAAAGGAG CCGTGAGGAA 
AAAAGAAGTA TCAAAGTTCA TGCCATGGTC TCCGTATTCC AATTTATTAT GAAACAAAGT 
TACATCTGTG CCCTCATAGC TATGATGGCC TGGAGCATCA CCTATCACAG CTGGCTGACC 
TTCGTGCTGC TGATCTGGTC GTGCACTCTT TGGATGATTC GCAACAGAAG AAAATATGCC 
ATGATCAGCT CTCCCTTCAT GGTGGTTTAT GGAAACCTAT TGTTGATATT ACAGTATATA 
TGGAGTTTTG AACTTCCTGA AATTAAAAAA GTTCCAGGAT TTTTAGAAAA GAAAGAGCCA 
GGAGAACTTG CTTCCAAAAT CCTTTTCACC ATTACTTTTT GGCTACTGCT GAGGCAGCAC 
CTCACAGAGC AAAAAGCTCT GCAAGAAAAG GAAGCTCTTT TATCGGAAGT CAAAATTGGC 
AGTCAGGAAA ATGAAGAAAA AGATGAGGAA CTTCAAGATA TACAAGTGGA AGGAGAGCCC 
AAAGAGGAGG AAGAAGAGGA AGCGAAGGAA GAGAAGCAAG AGAGAAAGAA GGTAGAGCAA 
GAGGAAGCTG AAGAAGAAGA TGAGCAGGAC ATCATGAAAG TCCTGGGCAA TCTGGTGGTG 
GCCATGTTCA TCAAGTACTG GATCTACGTC TGCGGAGGCA TGTTCTTCTT CGTCAGCTTC 
GAGGGTAAAA TCGTAATGTA CAAAATCATC TACATGGTGC TGTTCCTGTT CTGTGTGGCC 
CTATACCAGG TGCACTATGA ATGGTGGAGG AAAATTCTAA AATATTTTTG GATGTCAGTG 
GTTATTTACA CTATGCTGGT GCTTATCTTT ATATACACAT ATCAGTTTGA GAACTTCCCA 
GGCCTGTGGC AAAATATGAC TGGACTGAAA AAAGAAAAGC TTGAGGATCT TGGCTTAAAG 
CAGTTTACTG TGGCTGAACT ATTCACTCGC ATATTCATCC CAACCTCCTT TCTGCTGGTG 
TGCATTTTAC ACCTGCACTA CTTCCATGAC CGGTTCCTTG AACTCACAGA CCTCAAGTCC 
ATTCCCAGCA AAGAAGACAA CACCATCTAC AGACTGGCCC ACCCGGAAGG AAGCCTCCCG 
GACCTCACCA TGATGCATCT GACTGCCAGC CTGGAGAAGC CGGAGGTGAG GAAGTTGGCT 
GAGCCTGGGG AGGAGAAGCT TGAGGGCTAC TCTGAAAAAG CCCAGAAGGG TGATCTTGGG 
AAAGACAGCG AGGAGTCAGA GGAGGACGGA GAGGAAGAGG AGGAATCCGA GGAGGAGGAA 
GAAACATCAG ACTTAAGGAA CAAATGGCAC CTGGTGATTG ACCGCCTCAC TGTGCTCTTC 
TTAAAATTCC TGGAGTATTT TCACAAGCTG CAGGTGTTCA TGTGGTGGAT TTTGGAGTTG 
CACATCATCA AAATCGTTTC CTCTTACATT ATCTGGGTTT CTGTGAAAGA GGTGTCTCTG 
TTCAACTATG TATTTTTGAT TTCTTGGGCT TTTGCTCTGC CGTACGCCAA GCTGCGCCGT 
CTGGCTTCAA GTGTCTGCAC AGTCTGGACG TGTGTGATCA TCGTCTGCAA AATGTTGTAC 
CAGCTCCAAA CCATTAAGCC TGAGAACTTC TCTGTTAACT GTTCCTTGCC AAATGAAAAT 
CAAACAAACA TCCCCTTTAA TGAGTTGAAC AAGTCTCTGC TCTACAGCGC TCCTATCGAT 
CCTACAGAGT GGGTCGGCCT GCGGAAGTCT TCGCCTCTGC TAGTCTACCT GAGGAATAAC 
CTCCTGATGC TGGCTATCCT GGCCTTTGAA GTCACCATTT ACCGCCATCA GGAATACTAT 
CGAGGTCGAA ATAACCTGAC GGCCCCTGTG TCTAGAACTA TCTTTCATGA CATTACAAGA 
CTACATCTAG ATGATGGACT TATTAATTGT GCCAAATATT TCATTAATTA CTTCTTTTAC 
AAGTTTGGTC TGGAGACCTG TTTCCTAATG TCAGTTAACG TCATTGGCCA GCGAATGGAT 
TTCTATGCCA TGATCCACGC CTGCTGGCTG ATCGCTGTCT TATATAGACG CAGAAGGAAA 
GCCATCGCAG AGATCTGGCC CAAGTACTGC TGCTTCCTGG CATGCATCAT CACCTTCCAG 
TATTTCATCT GCATTGGCAT CCCACCTGCT CCTTGCCGAG ATTACCCGTG GAGATTCAAG 
GGTGCCAGCT TCAATGACAA CATCATAAAG TGGCTGTACT TCCCAGATTT CATTGTGCGG 
CCCAACCCTG TGTTTCTCGT CTATGACTTC ATGCTGCTTC TGTGTGCCTC CTTACAACGG 
CAGATTTTTG AGGATGAGAA CAAGGCTGCA GTGCGAATCA TGGCAGGTGA CAATGTCGAG 
ATCTGCATGA ACCTTGATGC GGCCTCCTTC AGCCAACATA ACCCTGTGCC AGATTTTATT 
CACTGCAGAT CTTACTTAGA CATGTCCAAA GTGATCATCT TCAGCTACCT CTTCTGGTTT 
GTGCTCACCA TCATCTTCAT CACTGGGACC ACCAGGATCA GCATCTTTTG CATGGGGTAC 
CTGGTGGCCT GTTTCTACTT CCTGCTCTTT GGGGGCGATT TGCTGTTGAA ACCCATCAAG 
AGCATCCTGC GCTACTGGGA CTGGCTGATC GCATACAACG TTTTTGTGAT TACGATGAAA 
AATATCCTGT CAATAGGAGC ATGTGGATAC ATTGGAACAT TGGTGCACAA TAGTTGTTGG 
TTGATCCAGG CTTTCAGCCT GGCCTGCACA GTCAAAGGCT ATCAAATGCC TGCTGCTAAT 
TCACCCTGTA CACTTCCCAG TGGGGAAGCA GGAATCATTT GGGACAGCAT ATGTTTTGCC 
TTCCTCCTGC TGCAAAGAAG AGTTTTCATG AGTTATTATT TTCTACATGT TGTGGCTGAT 
ATAAAAGCTT CCCAGATTCT GGCATCAAGA GGAGCTGAAC TTTTCCAGGC CACAATTGTA 
AAAGCTGTAA AGGCAAGAAT TGAGGAAGAG AAGAAGTCCA TGGACCAGCT GAAGCGACAG 
ATGGATCGCA TCAAGGCCAG GCAACAGAAA TATAAAAAGG GTAAGGAGAG GATGCTGAGC 
TTGACCCAGG AGCCAGGGGA AGGCCAGGAC ATGCAAAAAC TCTCTGAAGA GGATGATGAA 
AGAGAAGCAG ACAAACAGAA AGCCAAGGGC AAAAAAAAGC AGTGGTGGCG GCCTTGGGTT 
GATCATGCTT CCATGGTCAG GAGTGGAGAT TATTATTTGT TTGAAACGGA TAGTGAAGAG 
GAGGAAGAGG AAGAATTAAA GAAGGAAGAT GAAGAACCTC CACGAAGGTC AGCATTCCAG 
TTTGTTTATC AAGCCTGGAT TACTGATCCT AAAACAGCAC TCCGACAAAG ACACAAAGAG 
AAAAAAAGGT CTGCAAGAGA AGAACGGAAA CGAAGGCGGA AAGGATCCAA GGAGGGTCCT 
GTGGAATGGG AAGACCGGGA GGATGAACCA ATCAAAAAGA AATCCGATGG ACCAGATAAT 
ATCATCAAGA GGATATTTAA TATTTTGAAA TTTACCTGGG TCCTATTTCT GGCAACAGTG 
GACAGTTTCA CTACTTGGCT TAACTCCATT TCAAGGGAGC ATATTGATAT ATCTACAGTT 
CTGAGAATTG AACGATGCAT GCTGACCAGA GAAATTAAGA AGGGCAATGT TCCAACTCGG 
GAGAGCATCC ACATGTACTA TCAGAACCAC ATCATGAACC TTTCCAGAGA GTCGGGACTG 
GACACCATTG ACGAGCATCC CGGAGCTGCT TCAGGTGCAC AGACAGCCCA CAGGATGGAT 
AGTTTAGATT CACATGACAG TATCTCCAGC GAGCCCACGC AGTGTACCAT GCTGTACTCA 
CGCCAGGGGA CCACTGAGAC CATCGAGGAG GTGGAGGCTG AGCAGGAGGA GGAGGCAGGG 
AGCACGGCGC CTGAGCCCAG GGAGGCCAAG GAGTACGAGG CCACTGGGTA CGATGTGGGA 
GCCATGGGTG CCGAGGAGGC CAGCCTCACC CCAGAGGAAG AGCTGACACA GTTCTCCACC 
TTGGACGGGG ATGTGGAGGC CCCACCCTCC TACAGCAAGG CTGTGAGCTT CGAGCATCTG 
TCCTTCGGCT CGCAGGACGA CTCTGCAGGC AAGAACCGTA TGGCAGTCAG CCCGGACGAC 
AGCCGCACCG ACAAGCTGGG GTCCAGCATC TTACCTCCCC TGACCCATGA GCTGACGGCC 
AGCGAGCTGC TGCTGAAAAA GATGTTTCAC GACGATGAGC TTGAAGAGTC AGAGAAATTC 
TACGTGGGGC AGCCCCGATT TCTGCTGCTC TTCTATGCCA TGTACAATAC CCTGGTGGCC 
CGCTCGGAGA TGGTGTGCTA CTTCGTGATC ATCCTCAACC ACATGGTCTC TGCCTCCATG 
ATCACGCTCC TGCTTCCCAT CCTCATCTTC CTCTGGGCCA TGTTGTCCGT CCCCAGGCCC 
AGCCGCCGGT TCTGGATGAT GGCCATCGTC TATACTGAGG TGGCAATTGT AGTCAAGTAT 
TTCTTCCAAT TTGGGTTCTT TCCCTGGAAT AAGAATGTGG AGGTGAACAA AGATAAACCG 
TATCACCCCC CAAACATCAT AGGAGTGGAA AAGAAGGAAG GTTATGTTCT CTATGACCTC 
ATCCAGCTCC TGGCTCTGTT CTTTCATCGA TCAATTTTGA AGTGCCATGG CTTATGGGAT 
GAAGATGACA TGACTGAAAG TGGCATGGCC AGGGAGGAAT CAGATGATGA GCTCTCCCTC 
GGTCATGGCA GGAGGGACTC CTCCGATTCT CTCAAGTCCA TCAACCTGGC CGCGTCTGTG 
GAGTCAGTGC ATGTGACCTT CCCGGAGCAG CAGACAGCTG TCCGGAGGAA GCGCTCCGGC 
AGCAGCTCCG AGCCATCCCA GAGATCCAGC TTTTCTTCAA ACAGATCCCA AAGAGGCAGC 
ACAAGCACCC GAAACAGCAG TCAAAAAGGA AGCAGTGTTT TGAGTATTAA GCAAAAAGGC 
AAAAGGGAAC TTTATATGGA AAAGCTTCAA GAACATTTAA TCAAAGCAAA AGCCTTTACC 
ATAAAGAAGA CGCTGGAGAT CTATGTGCCC ATCAAACAGT TCTTTTACAA CCTCATCCAC 
CCGGAGTATA GCGCCGTGAC TGACGTGTAT GTACTCATGT TCCTGGCTGA CACTGTGGAC 
TTCATCATCA TTGTCTTCGG CTTTTGGGCC TTTGGGAAAC ACTCAGCAGC TGCAGACATC 
ACCTCTTCAC TGTCAGAGGA CCAGGTCCCG GGGCCGTTTT TGGTGATGGT CCTCATTCAG 
TTTGGAACCA TGGTGGTGGA CCGAGCCCTC TACCTCAGGA AGACTGTACT GGGAAAGGTC 
ATCTTCCAGG TCATTCTTGT GTTCGGAATT CACTTCTGGA TGTTCTTCAT CTTACCTGGT 
GTGACTGAGA GGAAATTCAG CCAGAACCTG GTTGCCCAGC TTTGGTACTT TGTGAAATGT 
GTTTACTTCG GGTTGTCTGC TTACCAGATC CGTTGTGGCT ACCCAACGCG AGTCCTGGGG 
AACTTCCTCA CCAAGAGCTA CAATTACGTC AACCTCTTCT TATTCCAAGG GTTTCGCCTC 
GTGCCCTTTT TGACTGAGCT GAGGGCAGTG ATGGACTGGG TGTGGACGGA CACAACTTTG 
AGCCTGTCCA GCTGGATCTG TGTGGAGGAC ATCTATGCTC ACATATTCAT CCTGAAGTGT 
TGGCGGGAGT CGGAGAAGAG ATACCCTCAG CCACGGGGCC AGAAGAAGAA GAAAGTGGTG 
AAGTATGGCA TGGGAGGAAT GATCATCGTC CTGCTCATCT GCATTGTCTG GTTTCCTCTT 
CTCTTCATGT CTTTGATCAA ATCTGTGGCT GGGGTCATCA ACCAGCCCCT GGACGTCTCC 
GTCACAATTA CCCTGGGAGG GTATCAGCCT ATTTTCACAA TGAGTGCCCA ACAAAGCCAG 
TTGAAAGTTA TGGACCAGCA GAGCTTTAAC AAATTTATAC AAGCTTTTTC TAGGGACACC 
GGTGCTATGC AATTTCTGGA AAATTATGAA AAAGAAGACA TAACAGTAGC AGAACTGGAA 
GGAAACTCAA ATTCTTTGTG GACCATCAGC CCACCCAGTA AGCAGAAAAT GATACACGAA 
CTCCTGGACC CCAATAGTAG CTTCTCTGTT GTTTTTTCAT GGAGTATTCA GAGAAACTTA 
AGTCTGGGTG CAAAATCGGA AATAGCAACA GATAAGCTTT CTTTTCCTCT TAAAAATATT 
ACTCGAAAGA ATATCGCTAA AATGATAGCA GGCAACAGCA CAGAAAGTTC AAAAACACCA 
GTGACCATAG AAAAGATTTA TCCATATTAT GTGAAAGCAC CTAGTGATTC TAACTCAAAA 
CCTATAAAGC AACTTTTATC TGAAAATAAT TTCATGGATA TTACCATCAT TTTGTCCAGA 
GACAATACAA CTAAATATAA CAGTGAGTGG TGGGTTCTCA ACCTGACTGG AAACAGAATA 
TACAATCCGA ACTCTCAGGC CCTGGAACTG GTGGTCTTCA ATGACAAAGT CAGTCCCCCA 
AGTCTGGGGT TCCTGGCTGG CTATGGTATT ATGGGATTAT ATGCTTCAGT TGTCCTTGTG 
ATTGGGAAAT TTGTCCGTGA ATTCTTCAGT GGGATTTCTC ACTCCATCAT GTTTGAAGAG 
CTTCCAAATG TGGATCGAAT TTTGAAGTTG TGCACAGATA TTTTTTTAGT TCGAGAGACA 
GGAGAACTGG AGCTAGAAGA AGATCTCTAT GCCAAATTAA TATTCCTATA TCGCTCACCA 
GAGACAATGA TCAAATGGAC TAGAGAAAAA ACAAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071351.2
CDS	175..8433
Translation	MASEVVCGLIFRLLLPICLAVACAFRYNGLSFVYLIYLLLIPLFSEPTKTTMQGHTGRLLKSLCFISLSFLLLHIIFHITLVSLEAQHRIAPGYNCSTWEKTFRQIGFESLKGADAGNGIRVFVPDIGMFIASLTIWLLCRNIVQKPVTDEAAQSNPEFENEELAEGEKIDSEEALIYEEDFNGGDGVEGELEESTKLKMFRRLASVASKLKEFIGNMITTAGKVVVTILLGSSGMMLPSLTSSVYFFVFLGLCTWWSWCRTFDPLLFSCLCVLLAIFTAGHLIGLYLYQFQFFQEAVPPNDYYARLFGIKSVIQTDCSSTWKIIVNPDLSWYHHANPILLLVMYYTLATLIRIWLQEPLVQDEGTKEEDKALACSPIQITAGRRRSLWYATHYPTDERKLLSMTQDDYKPSDGLLVTVNGNPVDYHTIHPSLPMENGPGKADLYSTPQYRWEPSDESSEKREEEEEEKEEFEEERSREEKRSIKVHAMVSVFQFIMKQSYICALIAMMAWSITYHSWLTFVLLIWSCTLWMIRNRRKYAMISSPFMVVYGNLLLILQYIWSFELPEIKKVPGFLEKKEPGELASKILFTITFWLLLRQHLTEQKALQEKEALLSEVKIGSQENEEKDEELQDIQVEGEPKEEEEEEAKEEKQERKKVEQEEAEEEDEQDIMKVLGNLVVAMFIKYWIYVCGGMFFFVSFEGKIVMYKIIYMVLFLFCVALYQVHYEWWRKILKYFWMSVVIYTMLVLIFIYTYQFENFPGLWQNMTGLKKEKLEDLGLKQFTVAELFTRIFIPTSFLLVCILHLHYFHDRFLELTDLKSIPSKEDNTIYRLAHPEGSLPDLTMMHLTASLEKPEVRKLAEPGEEKLEGYSEKAQKGDLGKDSEESEEDGEEEEESEEEEETSDLRNKWHLVIDRLTVLFLKFLEYFHKLQVFMWWILELHIIKIVSSYIIWVSVKEVSLFNYVFLISWAFALPYAKLRRLASSVCTVWTCVIIVCKMLYQLQTIKPENFSVNCSLPNENQTNIPFNELNKSLLYSAPIDPTEWVGLRKSSPLLVYLRNNLLMLAILAFEVTIYRHQEYYRGRNNLTAPVSRTIFHDITRLHLDDGLINCAKYFINYFFYKFGLETCFLMSVNVIGQRMDFYAMIHACWLIAVLYRRRRKAIAEIWPKYCCFLACIITFQYFICIGIPPAPCRDYPWRFKGASFNDNIIKWLYFPDFIVRPNPVFLVYDFMLLLCASLQRQIFEDENKAAVRIMAGDNVEICMNLDAASFSQHNPVPDFIHCRSYLDMSKVIIFSYLFWFVLTIIFITGTTRISIFCMGYLVACFYFLLFGGDLLLKPIKSILRYWDWLIAYNVFVITMKNILSIGACGYIGTLVHNSCWLIQAFSLACTVKGYQMPAANSPCTLPSGEAGIIWDSICFAFLLLQRRVFMSYYFLHVVADIKASQILASRGAELFQATIVKAVKARIEEEKKSMDQLKRQMDRIKARQQKYKKGKERMLSLTQEPGEGQDMQKLSEEDDEREADKQKAKGKKKQWWRPWVDHASMVRSGDYYLFETDSEEEEEEELKKEDEEPPRRSAFQFVYQAWITDPKTALRQRHKEKKRSAREERKRRRKGSKEGPVEWEDREDEPIKKKSDGPDNIIKRIFNILKFTWVLFLATVDSFTTWLNSISREHIDISTVLRIERCMLTREIKKGNVPTRESIHMYYQNHIMNLSRESGLDTIDEHPGAASGAQTAHRMDSLDSHDSISSEPTQCTMLYSRQGTTETIEEVEAEQEEEAGSTAPEPREAKEYEATGYDVGAMGAEEASLTPEEELTQFSTLDGDVEAPPSYSKAVSFEHLSFGSQDDSAGKNRMAVSPDDSRTDKLGSSILPPLTHELTASELLLKKMFHDDELEESEKFYVGQPRFLLLFYAMYNTLVARSEMVCYFVIILNHMVSASMITLLLPILIFLWAMLSVPRPSRRFWMMAIVYTEVAIVVKYFFQFGFFPWNKNVEVNKDKPYHPPNIIGVEKKEGYVLYDLIQLLALFFHRSILKCHGLWDEDDMTESGMAREESDDELSLGHGRRDSSDSLKSINLAASVESVHVTFPEQQTAVRRKRSGSSSEPSQRSSFSSNRSQRGSTSTRNSSQKGSSVLSIKQKGKRELYMEKLQEHLIKAKAFTIKKTLEIYVPIKQFFYNLIHPEYSAVTDVYVLMFLADTVDFIIIVFGFWAFGKHSAAADITSSLSEDQVPGPFLVMVLIQFGTMVVDRALYLRKTVLGKVIFQVILVFGIHFWMFFILPGVTERKFSQNLVAQLWYFVKCVYFGLSAYQIRCGYPTRVLGNFLTKSYNYVNLFLFQGFRLVPFLTELRAVMDWVWTDTTLSLSSWICVEDIYAHIFILKCWRESEKRYPQPRGQKKKKVVKYGMGGMIIVLLICIVWFPLLFMSLIKSVAGVINQPLDVSVTITLGGYQPIFTMSAQQSQLKVMDQQSFNKFIQAFSRDTGAMQFLENYEKEDITVAELEGNSNSLWTISPPSKQKMIHELLDPNSSFSVVFSWSIQRNLSLGAKSEIATDKLSFPLKNITRKNIAKMIAGNSTESSKTPVTIEKIYPYYVKAPSDSNSKPIKQLLSENNFMDITIILSRDNTTKYNSEWWVLNLTGNRIYNPNSQALELVVFNDKVSPPSLGFLAGYGIMGLYASVVLVIGKFVREFFSGISHSIMFEELPNVDRILKLCTDIFLVRETGELELEEDLYAKLIFLYRSPETMIKWTREKTN

Target ORF information:

RefSeq Version	NM_022068.3
Organism	Homo sapiens(human)
Definition	Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022068.3

ORF Insert Sequence:

ATGGCCTCAG AAGTGGTGTG CGGGCTCATC TTCAGGCTGC TGCTGCCCAT CTGCCTGGCA 
GTAGCATGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC 
ATTCCTCTGT TCTCAGAACC AACAAAAACG ACGATGCAAG GACATACGGG ACGGTTATTA 
AAGTCTCTGT GCTTCATCAG TCTTTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG 
TTGGTGAGCC TTGAAGCTCA ACATCGTATT GCACCTGGCT ACAACTGCTC AACATGGGAA 
AAGACATTCC GGCAGATCGG CTTTGAAAGC TTAAAGGGAG CTGATGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCCTCTGT 
AGAAACATTG TTCAGAAACC TGTGACAGAC GAAGCAGCAC AGAGTAACCC GGAGTTTGAA 
AATGAAGAAT TGGCTGAAGG AGAAAAAATT GATTCAGAAG AGGCACTGAT CTATGAAGAG 
GATTTCAATG GAGGAGATGG TGTTGAAGGC GAGTTGGAAG AAAGCACGAA GTTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCCTCTAAG CTCAAGGAGT TCATTGGCAA CATGATCACC 
ACTGCTGGGA AAGTCGTTGT TACCATCTTA CTGGGCTCCT CGGGCATGAT GTTGCCGTCT 
TTGACATCAT CTGTGTATTT TTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACTGCT 
GGACATTTGA TTGGACTTTA TTTATACCAG TTCCAATTCT TTCAAGAGGC AGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAGTCAGTAA TTCAAACGGA CTGTTCAAGT 
ACTTGGAAGA TCATAGTGAA CCCGGACCTG TCGTGGTACC ACCACGCCAA CCCTATCCTC 
CTGCTGGTGA TGTACTACAC TCTGGCCACT CTGATCCGCA TCTGGCTGCA AGAGCCCCTT 
GTGCAGGATG AGGGGACCAA AGAAGAGGAC AAAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACAGCGGGGA GGAGGCGGAG CCTGTGGTAC GCAACCCATT ACCCCACTGA TGAGAGAAAA 
CTTTTATCCA TGACCCAGGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC 
GGCAACCCCG TGGATTACCA CACCATCCAC CCAAGCCTGC CCATGGAGAA CGGCCCTGGC 
AAAGCCGACC TCTACTCCAC CCCTCAGTAC CGGTGGGAGC CCTCTGATGA ATCCTCAGAA 
AAGCGAGAGG AGGAAGAGGA AGAGAAAGAA GAATTTGAAG AAGAAAGGAG CCGTGAGGAA 
AAAAGAAGTA TCAAAGTTCA TGCCATGGTC TCCGTATTCC AATTTATTAT GAAACAAAGT 
TACATCTGTG CCCTCATAGC TATGATGGCC TGGAGCATCA CCTATCACAG CTGGCTGACC 
TTCGTGCTGC TGATCTGGTC GTGCACTCTT TGGATGATTC GCAACAGAAG AAAATATGCC 
ATGATCAGCT CTCCCTTCAT GGTGGTTTAT GGAAACCTAT TGTTGATATT ACAGTATATA 
TGGAGTTTTG AACTTCCTGA AATTAAAAAA GTTCCAGGAT TTTTAGAAAA GAAAGAGCCA 
GGAGAACTTG CTTCCAAAAT CCTTTTCACC ATTACTTTTT GGCTACTGCT GAGGCAGCAC 
CTCACAGAGC AAAAAGCTCT GCAAGAAAAG GAAGCTCTTT TATCGGAAGT CAAAATTGGC 
AGTCAGGAAA ATGAAGAAAA AGATGAGGAA CTTCAAGATA TACAAGTGGA AGGAGAGCCC 
AAAGAGGAGG AAGAAGAGGA AGCGAAGGAA GAGAAGCAAG AGAGAAAGAA GGTAGAGCAA 
GAGGAAGCTG AAGAAGAAGA TGAGCAGGAC ATCATGAAAG TCCTGGGCAA TCTGGTGGTG 
GCCATGTTCA TCAAGTACTG GATCTACGTC TGCGGAGGCA TGTTCTTCTT CGTCAGCTTC 
GAGGGTAAAA TCGTAATGTA CAAAATCATC TACATGGTGC TGTTCCTGTT CTGTGTGGCC 
CTATACCAGG TGCACTATGA ATGGTGGAGG AAAATTCTAA AATATTTTTG GATGTCAGTG 
GTTATTTACA CTATGCTGGT GCTTATCTTT ATATACACAT ATCAGTTTGA GAACTTCCCA 
GGCCTGTGGC AAAATATGAC TGGACTGAAA AAAGAAAAGC TTGAGGATCT TGGCTTAAAG 
CAGTTTACTG TGGCTGAACT ATTCACTCGC ATATTCATCC CAACCTCCTT TCTGCTGGTG 
TGCATTTTAC ACCTGCACTA CTTCCATGAC CGGTTCCTTG AACTCACAGA CCTCAAGTCC 
ATTCCCAGCA AAGAAGACAA CACCATCTAC AGACTGGCCC ACCCGGAAGG AAGCCTCCCG 
GACCTCACCA TGATGCATCT GACTGCCAGC CTGGAGAAGC CGGAGGTGAG GAAGTTGGCT 
GAGCCTGGGG AGGAGAAGCT TGAGGGCTAC TCTGAAAAAG CCCAGAAGGG TGATCTTGGG 
AAAGACAGCG AGGAGTCAGA GGAGGACGGA GAGGAAGAGG AGGAATCCGA GGAGGAGGAA 
GAAACATCAG ACTTAAGGAA CAAATGGCAC CTGGTGATTG ACCGCCTCAC TGTGCTCTTC 
TTAAAATTCC TGGAGTATTT TCACAAGCTG CAGGTGTTCA TGTGGTGGAT TTTGGAGTTG 
CACATCATCA AAATCGTTTC CTCTTACATT ATCTGGGTTT CTGTGAAAGA GGTGTCTCTG 
TTCAACTATG TATTTTTGAT TTCTTGGGCT TTTGCTCTGC CGTACGCCAA GCTGCGCCGT 
CTGGCTTCAA GTGTCTGCAC AGTCTGGACG TGTGTGATCA TCGTCTGCAA AATGTTGTAC 
CAGCTCCAAA CCATTAAGCC TGAGAACTTC TCTGTTAACT GTTCCTTGCC AAATGAAAAT 
CAAACAAACA TCCCCTTTAA TGAGTTGAAC AAGTCTCTGC TCTACAGCGC TCCTATCGAT 
CCTACAGAGT GGGTCGGCCT GCGGAAGTCT TCGCCTCTGC TAGTCTACCT GAGGAATAAC 
CTCCTGATGC TGGCTATCCT GGCCTTTGAA GTCACCATTT ACCGCCATCA GGAATACTAT 
CGAGGTCGAA ATAACCTGAC GGCCCCTGTG TCTAGAACTA TCTTTCATGA CATTACAAGA 
CTACATCTAG ATGATGGACT TATTAATTGT GCCAAATATT TCATTAATTA CTTCTTTTAC 
AAGTTTGGTC TGGAGACCTG TTTCCTAATG TCAGTTAACG TCATTGGCCA GCGAATGGAT 
TTCTATGCCA TGATCCACGC CTGCTGGCTG ATCGCTGTCT TATATAGACG CAGAAGGAAA 
GCCATCGCAG AGATCTGGCC CAAGTACTGC TGCTTCCTGG CATGCATCAT CACCTTCCAG 
TATTTCATCT GCATTGGCAT CCCACCTGCT CCTTGCCGAG ATTACCCGTG GAGATTCAAG 
GGTGCCAGCT TCAATGACAA CATCATAAAG TGGCTGTACT TCCCAGATTT CATTGTGCGG 
CCCAACCCTG TGTTTCTCGT CTATGACTTC ATGCTGCTTC TGTGTGCCTC CTTACAACGG 
CAGATTTTTG AGGATGAGAA CAAGGCTGCA GTGCGAATCA TGGCAGGTGA CAATGTCGAG 
ATCTGCATGA ACCTTGATGC GGCCTCCTTC AGCCAACATA ACCCTGTGCC AGATTTTATT 
CACTGCAGAT CTTACTTAGA CATGTCCAAA GTGATCATCT TCAGCTACCT CTTCTGGTTT 
GTGCTCACCA TCATCTTCAT CACTGGGACC ACCAGGATCA GCATCTTTTG CATGGGGTAC 
CTGGTGGCCT GTTTCTACTT CCTGCTCTTT GGGGGCGATT TGCTGTTGAA ACCCATCAAG 
AGCATCCTGC GCTACTGGGA CTGGCTGATC GCATACAACG TTTTTGTGAT TACGATGAAA 
AATATCCTGT CAATAGGAGC ATGTGGATAC ATTGGAACAT TGGTGCACAA TAGTTGTTGG 
TTGATCCAGG CTTTCAGCCT GGCCTGCACA GTCAAAGGCT ATCAAATGCC TGCTGCTAAT 
TCACCCTGTA CACTTCCCAG TGGGGAAGCA GGAATCATTT GGGACAGCAT ATGTTTTGCC 
TTCCTCCTGC TGCAAAGAAG AGTTTTCATG AGTTATTATT TTCTACATGT TGTGGCTGAT 
ATAAAAGCTT CCCAGATTCT GGCATCAAGA GGAGCTGAAC TTTTCCAGGC CACAATTGTA 
AAAGCTGTAA AGGCAAGAAT TGAGGAAGAG AAGAAGTCCA TGGACCAGCT GAAGCGACAG 
ATGGATCGCA TCAAGGCCAG GCAACAGAAA TATAAAAAGG GTAAGGAGAG GATGCTGAGC 
TTGACCCAGG AGCCAGGGGA AGGCCAGGAC ATGCAAAAAC TCTCTGAAGA GGATGATGAA 
AGAGAAGCAG ACAAACAGAA AGCCAAGGGC AAAAAAAAGC AGTGGTGGCG GCCTTGGGTT 
GATCATGCTT CCATGGTCAG GAGTGGAGAT TATTATTTGT TTGAAACGGA TAGTGAAGAG 
GAGGAAGAGG AAGAATTAAA GAAGGAAGAT GAAGAACCTC CACGAAGGTC AGCATTCCAG 
TTTGTTTATC AAGCCTGGAT TACTGATCCT AAAACAGCAC TCCGACAAAG ACACAAAGAG 
AAAAAAAGGT CTGCAAGAGA AGAACGGAAA CGAAGGCGGA AAGGATCCAA GGAGGGTCCT 
GTGGAATGGG AAGACCGGGA GGATGAACCA ATCAAAAAGA AATCCGATGG ACCAGATAAT 
ATCATCAAGA GGATATTTAA TATTTTGAAA TTTACCTGGG TCCTATTTCT GGCAACAGTG 
GACAGTTTCA CTACTTGGCT TAACTCCATT TCAAGGGAGC ATATTGATAT ATCTACAGTT 
CTGAGAATTG AACGATGCAT GCTGACCAGA GAAATTAAGA AGGGCAATGT TCCAACTCGG 
GAGAGCATCC ACATGTACTA TCAGAACCAC ATCATGAACC TTTCCAGAGA GTCGGGACTG 
GACACCATTG ACGAGCATCC CGGAGCTGCT TCAGGTGCAC AGACAGCCCA CAGGATGGAT 
AGTTTAGATT CACATGACAG TATCTCCAGC GAGCCCACGC AGTGTACCAT GCTGTACTCA 
CGCCAGGGGA CCACTGAGAC CATCGAGGAG GTGGAGGCTG AGCAGGAGGA GGAGGCAGGG 
AGCACGGCGC CTGAGCCCAG GGAGGCCAAG GAGTACGAGG CCACTGGGTA CGATGTGGGA 
GCCATGGGTG CCGAGGAGGC CAGCCTCACC CCAGAGGAAG AGCTGACACA GTTCTCCACC 
TTGGACGGGG ATGTGGAGGC CCCACCCTCC TACAGCAAGG CTGTGAGCTT CGAGCATCTG 
TCCTTCGGCT CGCAGGACGA CTCTGCAGGC AAGAACCGTA TGGCAGTCAG CCCGGACGAC 
AGCCGCACCG ACAAGCTGGG GTCCAGCATC TTACCTCCCC TGACCCATGA GCTGACGGCC 
AGCGAGCTGC TGCTGAAAAA GATGTTTCAC GACGATGAGC TTGAAGAGTC AGAGAAATTC 
TACGTGGGGC AGCCCCGATT TCTGCTGCTC TTCTATGCCA TGTACAATAC CCTGGTGGCC 
CGCTCGGAGA TGGTGTGCTA CTTCGTGATC ATCCTCAACC ACATGGTCTC TGCCTCCATG 
ATCACGCTCC TGCTTCCCAT CCTCATCTTC CTCTGGGCCA TGTTGTCCGT CCCCAGGCCC 
AGCCGCCGGT TCTGGATGAT GGCCATCGTC TATACTGAGG TGGCAATTGT AGTCAAGTAT 
TTCTTCCAAT TTGGGTTCTT TCCCTGGAAT AAGAATGTGG AGGTGAACAA AGATAAACCG 
TATCACCCCC CAAACATCAT AGGAGTGGAA AAGAAGGAAG GTTATGTTCT CTATGACCTC 
ATCCAGCTCC TGGCTCTGTT CTTTCATCGA TCAATTTTGA AGTGCCATGG CTTATGGGAT 
GAAGATGACA TGACTGAAAG TGGCATGGCC AGGGAGGAAT CAGATGATGA GCTCTCCCTC 
GGTCATGGCA GGAGGGACTC CTCCGATTCT CTCAAGTCCA TCAACCTGGC CGCGTCTGTG 
GAGTCAGTGC ATGTGACCTT CCCGGAGCAG CAGACAGCTG TCCGGAGGAA GCGCTCCGGC 
AGCAGCTCCG AGCCATCCCA GAGATCCAGC TTTTCTTCAA ACAGATCCCA AAGAGGCAGC 
ACAAGCACCC GAAACAGCAG TCAAAAAGGA AGCAGTGTTT TGAGTATTAA GCAAAAAGGC 
AAAAGGGAAC TTTATATGGA AAAGCTTCAA GAACATTTAA TCAAAGCAAA AGCCTTTACC 
ATAAAGAAGA CGCTGGAGAT CTATGTGCCC ATCAAACAGT TCTTTTACAA CCTCATCCAC 
CCGGAGTATA GCGCCGTGAC TGACGTGTAT GTACTCATGT TCCTGGCTGA CACTGTGGAC 
TTCATCATCA TTGTCTTCGG CTTTTGGGCC TTTGGGAAAC ACTCAGCAGC TGCAGACATC 
ACCTCTTCAC TGTCAGAGGA CCAGGTCCCG GGGCCGTTTT TGGTGATGGT CCTCATTCAG 
TTTGGAACCA TGGTGGTGGA CCGAGCCCTC TACCTCAGGA AGACTGTACT GGGAAAGGTC 
ATCTTCCAGG TCATTCTTGT GTTCGGAATT CACTTCTGGA TGTTCTTCAT CTTACCTGGT 
GTGACTGAGA GGAAATTCAG CCAGAACCTG GTTGCCCAGC TTTGGTACTT TGTGAAATGT 
GTTTACTTCG GGTTGTCTGC TTACCAGATC CGTTGTGGCT ACCCAACGCG AGTCCTGGGG 
AACTTCCTCA CCAAGAGCTA CAATTACGTC AACCTCTTCT TATTCCAAGG GTTTCGCCTC 
GTGCCCTTTT TGACTGAGCT GAGGGCAGTG ATGGACTGGG TGTGGACGGA CACAACTTTG 
AGCCTGTCCA GCTGGATCTG TGTGGAGGAC ATCTATGCTC ACATATTCAT CCTGAAGTGT 
TGGCGGGAGT CGGAGAAGAG ATACCCTCAG CCACGGGGCC AGAAGAAGAA GAAAGTGGTG 
AAGTATGGCA TGGGAGGAAT GATCATCGTC CTGCTCATCT GCATTGTCTG GTTTCCTCTT 
CTCTTCATGT CTTTGATCAA ATCTGTGGCT GGGGTCATCA ACCAGCCCCT GGACGTCTCC 
GTCACAATTA CCCTGGGAGG GTATCAGCCT ATTTTCACAA TGAGTGCCCA ACAAAGCCAG 
TTGAAAGTTA TGGACCAGCA GAGCTTTAAC AAATTTATAC AAGCTTTTTC TAGGGACACC 
GGTGCTATGC AATTTCTGGA AAATTATGAA AAAGAAGACA TAACAGTAGC AGAACTGGAA 
GGAAACTCAA ATTCTTTGTG GACCATCAGC CCACCCAGTA AGCAGAAAAT GATACACGAA 
CTCCTGGACC CCAATAGTAG CTTCTCTGTT GTTTTTTCAT GGAGTATTCA GAGAAACTTA 
AGTCTGGGTG CAAAATCGGA AATAGCAACA GATAAGCTTT CTTTTCCTCT TAAAAATATT 
ACTCGAAAGA ATATCGCTAA AATGATAGCA GGCAACAGCA CAGAAAGTTC AAAAACACCA 
GTGACCATAG AAAAGATTTA TCCATATTAT GTGAAAGCAC CTAGTGATTC TAACTCAAAA 
CCTATAAAGC AACTTTTATC TGAAAATAAT TTCATGGATA TTACCATCAT TTTGTCCAGA 
GACAATACAA CTAAATATAA CAGTGAGTGG TGGGTTCTCA ACCTGACTGG AAACAGAATA 
TACAATCCGA ACTCTCAGGC CCTGGAACTG GTGGTCTTCA ATGACAAAGT CAGTCCCCCA 
AGTCTGGGGT TCCTGGCTGG CTATGGTATT ATGGGATTAT ATGCTTCAGT TGTCCTTGTG 
ATTGGGAAAT TTGTCCGTGA ATTCTTCAGT GGGATTTCTC ACTCCATCAT GTTTGAAGAG 
CTTCCAAATG TGGATCGAAT TTTGAAGTTG TGCACAGATA TTTTTTTAGT TCGAGAGACA 
GGAGAACTGG AGCTAGAAGA AGATCTCTAT GCCAAATTAA TATTCCTATA TCGCTCACCA 
GAGACAATGA TCAAATGGAC TAGAGAAAAA ACAAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIEZO2 ( XM_011525723.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524025.1 Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X1, mRNA.

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CloneID	OHu71131
Clone ID Related Accession (Same CDS sequence)	XM_011525723.2 , XM_011525723.3
Accession Version	XM_011525723.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	piezo-type mechanosensitive ion channel component 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525723.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG AAGTGGTGTG CGGGCTCATC TTCAGGCTGC TGCTGCCCAT CTGCCTGGCA 
GTAGCATGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC 
ATTCCTCTGT TCTCAGAACC AACAAAAACG ACGATGCAAG GACATACGGG ACGGTTATTA 
AAGTCTCTGT GCTTCATCAG TCTTTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG 
TTGGTGAGCC TTGAAGCTCA ACATCGTATT GCACCTGGCT ACAACTGCTC AACATGGGAA 
AAGACATTCC GGCAGATCGG CTTTGAAAGC TTAAAGGGAG CTGATGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCCTCTGT 
AGAAACATTG TTCAGAAACC TGTGACAGAC GAAGCAGCAC AGAGTAACCC GGAGTTTGAA 
AATGAAGAAT TGGCTGAAGG AGAAAAAATT GATTCAGAAG AGGCACTGAT CTATGAAGAG 
GATTTCAATG GAGGAGATGG TGTTGAAGGC GAGTTGGAAG AAAGCACGAA GTTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCCTCTAAG CTCAAGGAGT TCATTGGCAA CATGATCACC 
ACTGCTGGGA AAGTCGTTGT TACCATCTTA CTGGGCTCCT CGGGCATGAT GTTGCCGTCT 
TTGACATCAT CTGTGTATTT TTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACTGCT 
GGACATTTGA TTGGACTTTA TTTATACCAG TTCCAATTCT TTCAAGAGGC AGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAGTCAGTAA TTCAAACGGA CTGTTCAAGT 
ACTTGGAAGA TCATAGTGAA CCCGGACCTG TCGTGGTACC ACCACGCCAA CCCTATCCTC 
CTGCTGGTGA TGTACTACAC TCTGGCCACT CTGATCCGCA TCTGGCTGCA AGAGCCCCTT 
GTGCAGGATG AGGGGACCAA AGAAGAGGAC AAAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACAGCGGGGA GGAGGCGGAG CCTGTGGTAC GCAACCCATT ACCCCACTGA TGAGAGAAAA 
CTTTTATCCA TGACCCAGGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC 
GGCAACCCCG TGGATTACCA CACCATCCAC CCAAGCCTGC CCATGGAGAA CGGCCCTGGC 
AAAGCCGACC TCTACTCCAC CCCTCAGTAC CGGTGGGAGC CCTCTGATGA ATCCTCAGAA 
AAGCGAGAGG AGGAAGAGGA AGAGAAAGAA GAATTTGAAG AAGAAAGGAG CCGTGAGGAA 
AAAAGAAGTA TCAAAGTTCA TGCCATGGTC TCCGTATTCC AATTTATTAT GAAACAAAGT 
TACATCTGTG CCCTCATAGC TATGATGGCC TGGAGCATCA CCTATCACAG CTGGCTGACC 
TTCGTGCTGC TGATCTGGTC GTGCACTCTT TGGATGATTC GCAACAGAAG AAAATATGCC 
ATGATCAGCT CTCCCTTCAT GGTGGTTTAT GGAAACCTAT TGTTGATATT ACAGTATATA 
TGGAGTTTTG AACTTCCTGA AATTAAAAAA GTTCCAGGAT TTTTAGAAAA GAAAGAGCCA 
GGAGAACTTG CTTCCAAAAT CCTTTTCACC ATTACTTTTT GGCTACTGCT GAGGCAGCAC 
CTCACAGAGC AAAAAGCTCT GCAAGAAAAG GAAGCTCTTT TATCGGAAGT CAAAATTGGC 
AGTCAGGAAA ATGAAGAAAA AGATGAGGAA CTTCAAGATA TACAAGTGGA AGGAGAGCCC 
AAAGAGGAGG AAGAAGAGGA AGCGAAGGAA GAGAAGCAAG AGAGAAAGAA GGTAGAGCAA 
GAGGAAGCTG AAGAAGAAGA TGAGCAGGAC ATCATGAAAG TCCTGGGCAA TCTGGTGGTG 
GCCATGTTCA TCAAGTACTG GATCTACGTC TGCGGAGGCA TGTTCTTCTT CGTCAGCTTC 
GAGGGTAAAA TCGTAATGTA CAAAATCATC TACATGGTGC TGTTCCTGTT CTGTGTGGCC 
CTATACCAGG TGCACTATGA ATGGTGGAGG AAAATTCTAA AATATTTTTG GATGTCAGTG 
GTTATTTACA CTATGCTGGT GCTTATCTTT ATATACACAT ATCAGTTTGA GAACTTCCCA 
GGCCTGTGGC AAAATATGAC TGGACTGAAA AAAGAAAAGC TTGAGGATCT TGGCTTAAAG 
CAGTTTACTG TGGCTGAACT ATTCACTCGC ATATTCATCC CAACCTCCTT TCTGCTGGTG 
TGCATTTTAC ACCTGCACTA CTTCCATGAC CGGTTCCTTG AACTCACAGA CCTCAAGTCC 
ATTCCCAGCA AAGAAGACAA CACCATCTAC AGCCATGCCA AAGTCAATGG TCGCGTATAT 
CTAATAATAA ATAGGATCTC ACACAGTCTT CCCACGGTTG CCAATCGACA GTTCGCATCA 
GTAACTCTCA GCATCAAGAA AAAATTACCA ATCCACCAAA ATGAACTGGC CCACCCGGAA 
GGAAGCCTCC CGGACCTCAC CATGATGCAT CTGACTGCCA GCCTGGAGAA GCCGGAGGTG 
AGGAAGTTGG CTGAGCCTGG GGAGGAGAAG CTTGAGGGCT ACTCTGAAAA AGCCCAGAAG 
GGTGATCTTG GGAAAGACAG CGAGGAGTCA GAGGAGGACG GAGAGGAAGA GGAGGAATCC 
GAGGAGGAGG AAGAAACATC AGACTTAAGG AACAAATGGC ACCTGGTGAT TGACCGCCTC 
ACTGTGCTCT TCTTAAAATT CCTGGAGTAT TTTCACAAGC TGCAGGTGTT CATGTGGTGG 
ATTTTGGAGT TGCACATCAT CAAAATCGTT TCCTCTTACA TTATCTGGGT TTCTGTGAAA 
GAGGTGTCTC TGTTCAACTA TGTATTTTTG ATTTCTTGGG CTTTTGCTCT GCCGTACGCC 
AAGCTGCGCC GTCTGGCTTC AAGTGTCTGC ACAGTCTGGA CGTGTGTGAT CATCGTCTGC 
AAAATGTTGT ACCAGCTCCA AACCATTAAG CCTGAGAACT TCTCTGTTAA CTGTTCCTTG 
CCAAATGAAA ATCAAACAAA CATCCCCTTT AATGAGTTGA ACAAGTCTCT GCTCTACAGC 
GCTCCTATCG ATCCTACAGA GTGGGTCGGC CTGCGGAAGT CTTCGCCTCT GCTAGTCTAC 
CTGAGGAATA ACCTCCTGAT GCTGGCTATC CTGGCCTTTG AAGTCACCAT TTACCGCCAT 
CAGGAATACT ATCGAGGTCG AAATAACCTG ACGGCCCCTG TGTCTAGAAC TATCTTTCAT 
GACATTACAA GACTACATCT AGATGATGGA CTTATTAATT GTGCCAAATA TTTCATTAAT 
TACTTCTTTT ACAAGTTTGG TCTGGAGACC TGTTTCCTAA TGTCAGTTAA CGTCATTGGC 
CAGCGAATGG ATTTCTATGC CATGATCCAC GCCTGCTGGC TGATCGCTGT CTTATATAGA 
CGCAGAAGGA AAGCCATCGC AGAGATCTGG CCCAAGTACT GCTGCTTCCT GGCATGCATC 
ATCACCTTCC AGTATTTCAT CTGCATTGGC ATCCCACCTG CTCCTTGCCG AGATTACCCG 
TGGAGATTCA AGGGTGCCAG CTTCAATGAC AACATCATAA AGTGGCTGTA CTTCCCAGAT 
TTCATTGTGC GGCCCAACCC TGTGTTTCTC GTCTATGACT TCATGCTGCT TCTGTGTGCC 
TCCTTACAAC GGCAGATTTT TGAGGATGAG AACAAGGCTG CAGTGCGAAT CATGGCAGGT 
GACAATGTCG AGATCTGCAT GAACCTTGAT GCGGCCTCCT TCAGCCAACA TAACCCTGTG 
CCAGATTTTA TTCACTGCAG ATCTTACTTA GACATGTCCA AAGTGATCAT CTTCAGCTAC 
CTCTTCTGGT TTGTGCTCAC CATCATCTTC ATCACTGGGA CCACCAGGAT CAGCATCTTT 
TGCATGGGGT ACCTGGTGGC CTGTTTCTAC TTCCTGCTCT TTGGGGGCGA TTTGCTGTTG 
AAACCCATCA AGAGCATCCT GCGCTACTGG GACTGGCTGA TCGCATACAA CGTTTTTGTG 
ATTACGATGA AAAATATCCT GTCAATAGGA GCATGTGGAT ACATTGGAAC ATTGGTGCAC 
AATAGTTGTT GGTTGATCCA GGCTTTCAGC CTGGCCTGCA CAGTCAAAGG CTATCAAATG 
CCTGCTGCTA ATTCACCCTG TACACTTCCC AGTGGGGAAG CAGGAATCAT TTGGGACAGC 
ATATGTTTTG CCTTCCTCCT GCTGCAAAGA AGAGTTTTCA TGAGTTATTA TTTTCTACAT 
GTTGTGGCTG ATATAAAAGC TTCCCAGATT CTGGCATCAA GAGGAGCTGA ACTTTTCCAG 
GCCACAATTG TAAAAGCTGT AAAGGCAAGA ATTGAGGAAG AGAAGAAGTC CATGGACCAG 
CTGAAGCGAC AGATGGATCG CATCAAGGCC AGGCAACAGA AATATAAAAA GGGTAAGGAG 
AGGATGCTGA GCTTGACCCA GGAGCCAGGG GAAGGCCAGG ACATGCAAAA ACTCTCTGAA 
GAGGATGATG AAAGAGAAGC AGACAAACAG AAAGCCAAGG GCAAAAAAAA GCAGTGGTGG 
CGGCCTTGGG TTGATCATGC TTCCATGGTC AGGAGTGGAG ATTATTATTT GTTTGAAACG 
GATAGTGAAG AGGAGGAAGA GGAAGAATTA AAGAAGGAAG ATGAAGAACC TCCACGAAGG 
TCAGCATTCC AGTTTGTTTA TCAAGCCTGG ATTACTGATC CTAAAACAGC ACTCCGACAA 
AGACACAAAG AGAAAAAAAG GTCTGCAAGA GAAGAACGGA AACGAAGGCG GAAAGGATCC 
AAGGAGGGTC CTGTGGAATG GGAAGACCGG GAGGATGAAC CAATCAAAAA GAAATCCGAT 
GGACCAGATA ATATCATCAA GAGGATATTT AATATTTTGA AATTTACCTG GGTCCTATTT 
CTGGCAACAG TGGACAGTTT CACTACTTGG CTTAACTCCA TTTCAAGGGA GCATATTGAT 
ATATCTACAG TTCTGAGAAT TGAACGATGC ATGCTGACCA GAGAAATTAA GAAGGGCAAT 
GTTCCAACTC GGGAGAGCAT CCACATGTAC TATCAGAACC ACATCATGAA CCTTTCCAGA 
GAGTCGGGAC TGGACACCAT TGACGAGCAT CCCGGAGCTG CTTCAGGTGC ACAGACAGCC 
CACAGGATGG ATAGTTTAGA TTCACATGAC AGTATCTCCA GCGAGCCCAC GCAGTGTACC 
ATGCTGTACT CACGCCAGGG GACCACTGAG ACCATCGAGG AGGTGGAGGC TGAGCAGGAG 
GAGGAGGCAG GGAGCACGGC GCCTGAGCCC AGGGAGGCCA AGGAGTACGA GGCCACTGGG 
TACGATGTGG GAGCCATGGG TGCCGAGGAG GCCAGCCTCA CCCCAGAGGA AGAGCTGACA 
CAGTTCTCCA CCTTGGACGG GGATGTGGAG GCCCCACCCT CCTACAGCAA GGCTGTGAGC 
TTCGAGCATC TGTCCTTCGG CTCGCAGGAC GACTCTGCAG GCAAGAACCG TATGGCAGTC 
AGCCCGGACG ACAGCCGCAC CGACAAGCTG GGGTCCAGCA TCTTACCTCC CCTGACCCAT 
GAGCTGACGG CCAGCGAGCT GCTGCTGAAA AAGATGTTTC ACGACGATGA GCTTGAAGAG 
TCAGAGAAAT TCTACGTGGG GCAGCCCCGA TTTCTGCTGC TCTTCTATGC CATGTACAAT 
ACCCTGGTGG CCCGCTCGGA GATGGTGTGC TACTTCGTGA TCATCCTCAA CCACATGGTC 
TCTGCCTCCA TGATCACGCT CCTGCTTCCC ATCCTCATCT TCCTCTGGGC CATGTTGTCC 
GTCCCCAGGC CCAGCCGCCG GTTCTGGATG ATGGCCATCG TCTATACTGA GGTGGCAATT 
GTAGTCAAGT ATTTCTTCCA ATTTGGGTTC TTTCCCTGGA ATAAGAATGT GGAGGTGAAC 
AAAGATAAAC CGTATCACCC CCCAAACATC ATAGGAGTGG AAAAGAAGGA AGGTTATGTT 
CTCTATGACC TCATCCAGCT CCTGGCTCTG TTCTTTCATC GATCAATTTT GAAGTGCCAT 
GGCTTATGGG ATGAAGATGA CATGACTGAA AGTGGCATGG CCAGGGAGGA ATCAGATGAT 
GAGCTCTCCC TCGGTCATGG CAGGAGGGAC TCCTCCGATT CTCTCAAGTC CATCAACCTG 
GCCGCGTCTG TGGAGTCAGT GCATGTGACC TTCCCGGAGC AGCAGACAGC TGTCCGGAGG 
AAGCGCTCCG GCAGCAGCTC CGAGCCATCC CAGAGATCCA GCTTTTCTTC AAACAGATCC 
CAAAGAGGCA GCACAAGCAC CCGAAACAGC AGTCAAAAAG GAAGCAGTGT TTTGAGTATT 
AAGCAAAAAG GCAAAAGGGA ACTTTATATG GAAAAGCTTC AAGAACATTT AATCAAAGCA 
AAAGCCTTTA CCATAAAGAA GACGCTGGAG ATCTATGTGC CCATCAAACA GTTCTTTTAC 
AACCTCATCC ACCCGGAGTA TAGCGCCGTG ACTGACGTGT ATGTACTCAT GTTCCTGGCT 
GACACTGTGG ACTTCATCAT CATTGTCTTC GGCTTTTGGG CCTTTGGGAA ACACTCAGCA 
GCTGCAGACA TCACCTCTTC ACTGTCAGAG GACCAGGTCC CGGGGCCGTT TTTGGTGATG 
GTCCTCATTC AGTTTGGAAC CATGGTGGTG GACCGAGCCC TCTACCTCAG GAAGACTGTA 
CTGGGAAAGG TCATCTTCCA GGTCATTCTT GTGTTCGGAA TTCACTTCTG GATGTTCTTC 
ATCTTACCTG GTGTGACTGA GAGGAAATTC AGCCAGAACC TGGTTGCCCA GCTTTGGTAC 
TTTGTGAAAT GTGTTTACTT CGGGTTGTCT GCTTACCAGA TCCGTTGTGG CTACCCAACG 
CGAGTCCTGG GGAACTTCCT CACCAAGAGC TACAATTACG TCAACCTCTT CTTATTCCAA 
GGGTTTCGCC TCGTGCCCTT TTTGACTGAG CTGAGGGCAG TGATGGACTG GGTGTGGACG 
GACACAACTT TGAGCCTGTC CAGCTGGATC TGTGTGGAGG ACATCTATGC TCACATATTC 
ATCCTGAAGT GTTGGCGGGA GTCGGAGAAG AGATACCCTC AGCCACGGGG CCAGAAGAAG 
AAGAAAGTGG TGAAGTATGG CATGGGAGGA ATGATCATCG TCCTGCTCAT CTGCATTGTC 
TGGTTTCCTC TTCTCTTCAT GTCTTTGATC AAATCTGTGG CTGGGGTCAT CAACCAGCCC 
CTGGACGTCT CCGTCACAAT TACCCTGGGA GGGTATCAGC CTATTTTCAC AATGAGTGCC 
CAACAAAGCC AGTTGAAAGT TATGGACCAG CAGAGCTTTA ACAAATTTAT ACAAGCTTTT 
TCTAGGGACA CCGGTGCTAT GCAATTTCTG GAAAATTATG AAAAAGAAGA CATAACAGTA 
GCAGAACTGG AAGGAAACTC AAATTCTTTG TGGACCATCA GCCCACCCAG TAAGCAGAAA 
ATGATACACG AACTCCTGGA CCCCAATAGT AGCTTCTCTG TTGTTTTTTC ATGGAGTATT 
CAGAGAAACT TAAGTCTGGG TGCAAAATCG GAAATAGCAA CAGATAAGCT TTCTTTTCCT 
CTTAAAAATA TTACTCGAAA GAATATCGCT AAAATGATAG CAGGCAACAG CACAGAAAGT 
TCAAAAACAC CAGTGACCAT AGAAAAGATT TATCCATATT ATGTGAAAGC ACCTAGTGAT 
TCTAACTCAA AACCTATAAA GCAACTTTTA TCTGAAAATA ATTTCATGGA TATTACCATC 
ATTTTGTCCA GAGACAATAC AACTAAATAT AACAGTGAGT GGTGGGTTCT CAACCTGACT 
GGAAACAGAA TATACAATCC GAACTCTCAG GCCCTGGAAC TGGTGGTCTT CAATGACAAA 
GTCAGTCCCC CAAGTCTGGG GTTCCTGGCT GGCTATGGTA TTATGGGATT ATATGCTTCA 
GTTGTCCTTG TGATTGGGAA ATTTGTCCGT GAATTCTTCA GTGGGATTTC TCACTCCATC 
ATGTTTGAAG AGCTTCCAAA TGTGGATCGA ATTTTGAAGT TGTGCACAGA TATTTTTTTA 
GTTCGAGAGA CAGGAGAACT GGAGCTAGAA GAAGATCTCT ATGCCAAATT AATATTCCTA 
TATCGCTCAC CAGAGACAAT GATCAAATGG ACTAGAGAAA AAACAAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524025.1
CDS	973..9363
Translation	MASEVVCGLIFRLLLPICLAVACAFRYNGLSFVYLIYLLLIPLFSEPTKTTMQGHTGRLLKSLCFISLSFLLLHIIFHITLVSLEAQHRIAPGYNCSTWEKTFRQIGFESLKGADAGNGIRVFVPDIGMFIASLTIWLLCRNIVQKPVTDEAAQSNPEFENEELAEGEKIDSEEALIYEEDFNGGDGVEGELEESTKLKMFRRLASVASKLKEFIGNMITTAGKVVVTILLGSSGMMLPSLTSSVYFFVFLGLCTWWSWCRTFDPLLFSCLCVLLAIFTAGHLIGLYLYQFQFFQEAVPPNDYYARLFGIKSVIQTDCSSTWKIIVNPDLSWYHHANPILLLVMYYTLATLIRIWLQEPLVQDEGTKEEDKALACSPIQITAGRRRSLWYATHYPTDERKLLSMTQDDYKPSDGLLVTVNGNPVDYHTIHPSLPMENGPGKADLYSTPQYRWEPSDESSEKREEEEEEKEEFEEERSREEKRSIKVHAMVSVFQFIMKQSYICALIAMMAWSITYHSWLTFVLLIWSCTLWMIRNRRKYAMISSPFMVVYGNLLLILQYIWSFELPEIKKVPGFLEKKEPGELASKILFTITFWLLLRQHLTEQKALQEKEALLSEVKIGSQENEEKDEELQDIQVEGEPKEEEEEEAKEEKQERKKVEQEEAEEEDEQDIMKVLGNLVVAMFIKYWIYVCGGMFFFVSFEGKIVMYKIIYMVLFLFCVALYQVHYEWWRKILKYFWMSVVIYTMLVLIFIYTYQFENFPGLWQNMTGLKKEKLEDLGLKQFTVAELFTRIFIPTSFLLVCILHLHYFHDRFLELTDLKSIPSKEDNTIYSHAKVNGRVYLIINRISHSLPTVANRQFASVTLSIKKKLPIHQNELAHPEGSLPDLTMMHLTASLEKPEVRKLAEPGEEKLEGYSEKAQKGDLGKDSEESEEDGEEEEESEEEEETSDLRNKWHLVIDRLTVLFLKFLEYFHKLQVFMWWILELHIIKIVSSYIIWVSVKEVSLFNYVFLISWAFALPYAKLRRLASSVCTVWTCVIIVCKMLYQLQTIKPENFSVNCSLPNENQTNIPFNELNKSLLYSAPIDPTEWVGLRKSSPLLVYLRNNLLMLAILAFEVTIYRHQEYYRGRNNLTAPVSRTIFHDITRLHLDDGLINCAKYFINYFFYKFGLETCFLMSVNVIGQRMDFYAMIHACWLIAVLYRRRRKAIAEIWPKYCCFLACIITFQYFICIGIPPAPCRDYPWRFKGASFNDNIIKWLYFPDFIVRPNPVFLVYDFMLLLCASLQRQIFEDENKAAVRIMAGDNVEICMNLDAASFSQHNPVPDFIHCRSYLDMSKVIIFSYLFWFVLTIIFITGTTRISIFCMGYLVACFYFLLFGGDLLLKPIKSILRYWDWLIAYNVFVITMKNILSIGACGYIGTLVHNSCWLIQAFSLACTVKGYQMPAANSPCTLPSGEAGIIWDSICFAFLLLQRRVFMSYYFLHVVADIKASQILASRGAELFQATIVKAVKARIEEEKKSMDQLKRQMDRIKARQQKYKKGKERMLSLTQEPGEGQDMQKLSEEDDEREADKQKAKGKKKQWWRPWVDHASMVRSGDYYLFETDSEEEEEEELKKEDEEPPRRSAFQFVYQAWITDPKTALRQRHKEKKRSAREERKRRRKGSKEGPVEWEDREDEPIKKKSDGPDNIIKRIFNILKFTWVLFLATVDSFTTWLNSISREHIDISTVLRIERCMLTREIKKGNVPTRESIHMYYQNHIMNLSRESGLDTIDEHPGAASGAQTAHRMDSLDSHDSISSEPTQCTMLYSRQGTTETIEEVEAEQEEEAGSTAPEPREAKEYEATGYDVGAMGAEEASLTPEEELTQFSTLDGDVEAPPSYSKAVSFEHLSFGSQDDSAGKNRMAVSPDDSRTDKLGSSILPPLTHELTASELLLKKMFHDDELEESEKFYVGQPRFLLLFYAMYNTLVARSEMVCYFVIILNHMVSASMITLLLPILIFLWAMLSVPRPSRRFWMMAIVYTEVAIVVKYFFQFGFFPWNKNVEVNKDKPYHPPNIIGVEKKEGYVLYDLIQLLALFFHRSILKCHGLWDEDDMTESGMAREESDDELSLGHGRRDSSDSLKSINLAASVESVHVTFPEQQTAVRRKRSGSSSEPSQRSSFSSNRSQRGSTSTRNSSQKGSSVLSIKQKGKRELYMEKLQEHLIKAKAFTIKKTLEIYVPIKQFFYNLIHPEYSAVTDVYVLMFLADTVDFIIIVFGFWAFGKHSAAADITSSLSEDQVPGPFLVMVLIQFGTMVVDRALYLRKTVLGKVIFQVILVFGIHFWMFFILPGVTERKFSQNLVAQLWYFVKCVYFGLSAYQIRCGYPTRVLGNFLTKSYNYVNLFLFQGFRLVPFLTELRAVMDWVWTDTTLSLSSWICVEDIYAHIFILKCWRESEKRYPQPRGQKKKKVVKYGMGGMIIVLLICIVWFPLLFMSLIKSVAGVINQPLDVSVTITLGGYQPIFTMSAQQSQLKVMDQQSFNKFIQAFSRDTGAMQFLENYEKEDITVAELEGNSNSLWTISPPSKQKMIHELLDPNSSFSVVFSWSIQRNLSLGAKSEIATDKLSFPLKNITRKNIAKMIAGNSTESSKTPVTIEKIYPYYVKAPSDSNSKPIKQLLSENNFMDITIILSRDNTTKYNSEWWVLNLTGNRIYNPNSQALELVVFNDKVSPPSLGFLAGYGIMGLYASVVLVIGKFVREFFSGISHSIMFEELPNVDRILKLCTDIFLVRETGELELEEDLYAKLIFLYRSPETMIKWTREKTN

Target ORF information:

RefSeq Version	XM_011525723.2
Organism	Homo sapiens(human)
Definition	Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525723.2

ORF Insert Sequence:

ATGGCCTCAG AAGTGGTGTG CGGGCTCATC TTCAGGCTGC TGCTGCCCAT CTGCCTGGCA 
GTAGCATGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC 
ATTCCTCTGT TCTCAGAACC AACAAAAACG ACGATGCAAG GACATACGGG ACGGTTATTA 
AAGTCTCTGT GCTTCATCAG TCTTTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG 
TTGGTGAGCC TTGAAGCTCA ACATCGTATT GCACCTGGCT ACAACTGCTC AACATGGGAA 
AAGACATTCC GGCAGATCGG CTTTGAAAGC TTAAAGGGAG CTGATGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCCTCTGT 
AGAAACATTG TTCAGAAACC TGTGACAGAC GAAGCAGCAC AGAGTAACCC GGAGTTTGAA 
AATGAAGAAT TGGCTGAAGG AGAAAAAATT GATTCAGAAG AGGCACTGAT CTATGAAGAG 
GATTTCAATG GAGGAGATGG TGTTGAAGGC GAGTTGGAAG AAAGCACGAA GTTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCCTCTAAG CTCAAGGAGT TCATTGGCAA CATGATCACC 
ACTGCTGGGA AAGTCGTTGT TACCATCTTA CTGGGCTCCT CGGGCATGAT GTTGCCGTCT 
TTGACATCAT CTGTGTATTT TTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACTGCT 
GGACATTTGA TTGGACTTTA TTTATACCAG TTCCAATTCT TTCAAGAGGC AGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAGTCAGTAA TTCAAACGGA CTGTTCAAGT 
ACTTGGAAGA TCATAGTGAA CCCGGACCTG TCGTGGTACC ACCACGCCAA CCCTATCCTC 
CTGCTGGTGA TGTACTACAC TCTGGCCACT CTGATCCGCA TCTGGCTGCA AGAGCCCCTT 
GTGCAGGATG AGGGGACCAA AGAAGAGGAC AAAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACAGCGGGGA GGAGGCGGAG CCTGTGGTAC GCAACCCATT ACCCCACTGA TGAGAGAAAA 
CTTTTATCCA TGACCCAGGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC 
GGCAACCCCG TGGATTACCA CACCATCCAC CCAAGCCTGC CCATGGAGAA CGGCCCTGGC 
AAAGCCGACC TCTACTCCAC CCCTCAGTAC CGGTGGGAGC CCTCTGATGA ATCCTCAGAA 
AAGCGAGAGG AGGAAGAGGA AGAGAAAGAA GAATTTGAAG AAGAAAGGAG CCGTGAGGAA 
AAAAGAAGTA TCAAAGTTCA TGCCATGGTC TCCGTATTCC AATTTATTAT GAAACAAAGT 
TACATCTGTG CCCTCATAGC TATGATGGCC TGGAGCATCA CCTATCACAG CTGGCTGACC 
TTCGTGCTGC TGATCTGGTC GTGCACTCTT TGGATGATTC GCAACAGAAG AAAATATGCC 
ATGATCAGCT CTCCCTTCAT GGTGGTTTAT GGAAACCTAT TGTTGATATT ACAGTATATA 
TGGAGTTTTG AACTTCCTGA AATTAAAAAA GTTCCAGGAT TTTTAGAAAA GAAAGAGCCA 
GGAGAACTTG CTTCCAAAAT CCTTTTCACC ATTACTTTTT GGCTACTGCT GAGGCAGCAC 
CTCACAGAGC AAAAAGCTCT GCAAGAAAAG GAAGCTCTTT TATCGGAAGT CAAAATTGGC 
AGTCAGGAAA ATGAAGAAAA AGATGAGGAA CTTCAAGATA TACAAGTGGA AGGAGAGCCC 
AAAGAGGAGG AAGAAGAGGA AGCGAAGGAA GAGAAGCAAG AGAGAAAGAA GGTAGAGCAA 
GAGGAAGCTG AAGAAGAAGA TGAGCAGGAC ATCATGAAAG TCCTGGGCAA TCTGGTGGTG 
GCCATGTTCA TCAAGTACTG GATCTACGTC TGCGGAGGCA TGTTCTTCTT CGTCAGCTTC 
GAGGGTAAAA TCGTAATGTA CAAAATCATC TACATGGTGC TGTTCCTGTT CTGTGTGGCC 
CTATACCAGG TGCACTATGA ATGGTGGAGG AAAATTCTAA AATATTTTTG GATGTCAGTG 
GTTATTTACA CTATGCTGGT GCTTATCTTT ATATACACAT ATCAGTTTGA GAACTTCCCA 
GGCCTGTGGC AAAATATGAC TGGACTGAAA AAAGAAAAGC TTGAGGATCT TGGCTTAAAG 
CAGTTTACTG TGGCTGAACT ATTCACTCGC ATATTCATCC CAACCTCCTT TCTGCTGGTG 
TGCATTTTAC ACCTGCACTA CTTCCATGAC CGGTTCCTTG AACTCACAGA CCTCAAGTCC 
ATTCCCAGCA AAGAAGACAA CACCATCTAC AGCCATGCCA AAGTCAATGG TCGCGTATAT 
CTAATAATAA ATAGGATCTC ACACAGTCTT CCCACGGTTG CCAATCGACA GTTCGCATCA 
GTAACTCTCA GCATCAAGAA AAAATTACCA ATCCACCAAA ATGAACTGGC CCACCCGGAA 
GGAAGCCTCC CGGACCTCAC CATGATGCAT CTGACTGCCA GCCTGGAGAA GCCGGAGGTG 
AGGAAGTTGG CTGAGCCTGG GGAGGAGAAG CTTGAGGGCT ACTCTGAAAA AGCCCAGAAG 
GGTGATCTTG GGAAAGACAG CGAGGAGTCA GAGGAGGACG GAGAGGAAGA GGAGGAATCC 
GAGGAGGAGG AAGAAACATC AGACTTAAGG AACAAATGGC ACCTGGTGAT TGACCGCCTC 
ACTGTGCTCT TCTTAAAATT CCTGGAGTAT TTTCACAAGC TGCAGGTGTT CATGTGGTGG 
ATTTTGGAGT TGCACATCAT CAAAATCGTT TCCTCTTACA TTATCTGGGT TTCTGTGAAA 
GAGGTGTCTC TGTTCAACTA TGTATTTTTG ATTTCTTGGG CTTTTGCTCT GCCGTACGCC 
AAGCTGCGCC GTCTGGCTTC AAGTGTCTGC ACAGTCTGGA CGTGTGTGAT CATCGTCTGC 
AAAATGTTGT ACCAGCTCCA AACCATTAAG CCTGAGAACT TCTCTGTTAA CTGTTCCTTG 
CCAAATGAAA ATCAAACAAA CATCCCCTTT AATGAGTTGA ACAAGTCTCT GCTCTACAGC 
GCTCCTATCG ATCCTACAGA GTGGGTCGGC CTGCGGAAGT CTTCGCCTCT GCTAGTCTAC 
CTGAGGAATA ACCTCCTGAT GCTGGCTATC CTGGCCTTTG AAGTCACCAT TTACCGCCAT 
CAGGAATACT ATCGAGGTCG AAATAACCTG ACGGCCCCTG TGTCTAGAAC TATCTTTCAT 
GACATTACAA GACTACATCT AGATGATGGA CTTATTAATT GTGCCAAATA TTTCATTAAT 
TACTTCTTTT ACAAGTTTGG TCTGGAGACC TGTTTCCTAA TGTCAGTTAA CGTCATTGGC 
CAGCGAATGG ATTTCTATGC CATGATCCAC GCCTGCTGGC TGATCGCTGT CTTATATAGA 
CGCAGAAGGA AAGCCATCGC AGAGATCTGG CCCAAGTACT GCTGCTTCCT GGCATGCATC 
ATCACCTTCC AGTATTTCAT CTGCATTGGC ATCCCACCTG CTCCTTGCCG AGATTACCCG 
TGGAGATTCA AGGGTGCCAG CTTCAATGAC AACATCATAA AGTGGCTGTA CTTCCCAGAT 
TTCATTGTGC GGCCCAACCC TGTGTTTCTC GTCTATGACT TCATGCTGCT TCTGTGTGCC 
TCCTTACAAC GGCAGATTTT TGAGGATGAG AACAAGGCTG CAGTGCGAAT CATGGCAGGT 
GACAATGTCG AGATCTGCAT GAACCTTGAT GCGGCCTCCT TCAGCCAACA TAACCCTGTG 
CCAGATTTTA TTCACTGCAG ATCTTACTTA GACATGTCCA AAGTGATCAT CTTCAGCTAC 
CTCTTCTGGT TTGTGCTCAC CATCATCTTC ATCACTGGGA CCACCAGGAT CAGCATCTTT 
TGCATGGGGT ACCTGGTGGC CTGTTTCTAC TTCCTGCTCT TTGGGGGCGA TTTGCTGTTG 
AAACCCATCA AGAGCATCCT GCGCTACTGG GACTGGCTGA TCGCATACAA CGTTTTTGTG 
ATTACGATGA AAAATATCCT GTCAATAGGA GCATGTGGAT ACATTGGAAC ATTGGTGCAC 
AATAGTTGTT GGTTGATCCA GGCTTTCAGC CTGGCCTGCA CAGTCAAAGG CTATCAAATG 
CCTGCTGCTA ATTCACCCTG TACACTTCCC AGTGGGGAAG CAGGAATCAT TTGGGACAGC 
ATATGTTTTG CCTTCCTCCT GCTGCAAAGA AGAGTTTTCA TGAGTTATTA TTTTCTACAT 
GTTGTGGCTG ATATAAAAGC TTCCCAGATT CTGGCATCAA GAGGAGCTGA ACTTTTCCAG 
GCCACAATTG TAAAAGCTGT AAAGGCAAGA ATTGAGGAAG AGAAGAAGTC CATGGACCAG 
CTGAAGCGAC AGATGGATCG CATCAAGGCC AGGCAACAGA AATATAAAAA GGGTAAGGAG 
AGGATGCTGA GCTTGACCCA GGAGCCAGGG GAAGGCCAGG ACATGCAAAA ACTCTCTGAA 
GAGGATGATG AAAGAGAAGC AGACAAACAG AAAGCCAAGG GCAAAAAAAA GCAGTGGTGG 
CGGCCTTGGG TTGATCATGC TTCCATGGTC AGGAGTGGAG ATTATTATTT GTTTGAAACG 
GATAGTGAAG AGGAGGAAGA GGAAGAATTA AAGAAGGAAG ATGAAGAACC TCCACGAAGG 
TCAGCATTCC AGTTTGTTTA TCAAGCCTGG ATTACTGATC CTAAAACAGC ACTCCGACAA 
AGACACAAAG AGAAAAAAAG GTCTGCAAGA GAAGAACGGA AACGAAGGCG GAAAGGATCC 
AAGGAGGGTC CTGTGGAATG GGAAGACCGG GAGGATGAAC CAATCAAAAA GAAATCCGAT 
GGACCAGATA ATATCATCAA GAGGATATTT AATATTTTGA AATTTACCTG GGTCCTATTT 
CTGGCAACAG TGGACAGTTT CACTACTTGG CTTAACTCCA TTTCAAGGGA GCATATTGAT 
ATATCTACAG TTCTGAGAAT TGAACGATGC ATGCTGACCA GAGAAATTAA GAAGGGCAAT 
GTTCCAACTC GGGAGAGCAT CCACATGTAC TATCAGAACC ACATCATGAA CCTTTCCAGA 
GAGTCGGGAC TGGACACCAT TGACGAGCAT CCCGGAGCTG CTTCAGGTGC ACAGACAGCC 
CACAGGATGG ATAGTTTAGA TTCACATGAC AGTATCTCCA GCGAGCCCAC GCAGTGTACC 
ATGCTGTACT CACGCCAGGG GACCACTGAG ACCATCGAGG AGGTGGAGGC TGAGCAGGAG 
GAGGAGGCAG GGAGCACGGC GCCTGAGCCC AGGGAGGCCA AGGAGTACGA GGCCACTGGG 
TACGATGTGG GAGCCATGGG TGCCGAGGAG GCCAGCCTCA CCCCAGAGGA AGAGCTGACA 
CAGTTCTCCA CCTTGGACGG GGATGTGGAG GCCCCACCCT CCTACAGCAA GGCTGTGAGC 
TTCGAGCATC TGTCCTTCGG CTCGCAGGAC GACTCTGCAG GCAAGAACCG TATGGCAGTC 
AGCCCGGACG ACAGCCGCAC CGACAAGCTG GGGTCCAGCA TCTTACCTCC CCTGACCCAT 
GAGCTGACGG CCAGCGAGCT GCTGCTGAAA AAGATGTTTC ACGACGATGA GCTTGAAGAG 
TCAGAGAAAT TCTACGTGGG GCAGCCCCGA TTTCTGCTGC TCTTCTATGC CATGTACAAT 
ACCCTGGTGG CCCGCTCGGA GATGGTGTGC TACTTCGTGA TCATCCTCAA CCACATGGTC 
TCTGCCTCCA TGATCACGCT CCTGCTTCCC ATCCTCATCT TCCTCTGGGC CATGTTGTCC 
GTCCCCAGGC CCAGCCGCCG GTTCTGGATG ATGGCCATCG TCTATACTGA GGTGGCAATT 
GTAGTCAAGT ATTTCTTCCA ATTTGGGTTC TTTCCCTGGA ATAAGAATGT GGAGGTGAAC 
AAAGATAAAC CGTATCACCC CCCAAACATC ATAGGAGTGG AAAAGAAGGA AGGTTATGTT 
CTCTATGACC TCATCCAGCT CCTGGCTCTG TTCTTTCATC GATCAATTTT GAAGTGCCAT 
GGCTTATGGG ATGAAGATGA CATGACTGAA AGTGGCATGG CCAGGGAGGA ATCAGATGAT 
GAGCTCTCCC TCGGTCATGG CAGGAGGGAC TCCTCCGATT CTCTCAAGTC CATCAACCTG 
GCCGCGTCTG TGGAGTCAGT GCATGTGACC TTCCCGGAGC AGCAGACAGC TGTCCGGAGG 
AAGCGCTCCG GCAGCAGCTC CGAGCCATCC CAGAGATCCA GCTTTTCTTC AAACAGATCC 
CAAAGAGGCA GCACAAGCAC CCGAAACAGC AGTCAAAAAG GAAGCAGTGT TTTGAGTATT 
AAGCAAAAAG GCAAAAGGGA ACTTTATATG GAAAAGCTTC AAGAACATTT AATCAAAGCA 
AAAGCCTTTA CCATAAAGAA GACGCTGGAG ATCTATGTGC CCATCAAACA GTTCTTTTAC 
AACCTCATCC ACCCGGAGTA TAGCGCCGTG ACTGACGTGT ATGTACTCAT GTTCCTGGCT 
GACACTGTGG ACTTCATCAT CATTGTCTTC GGCTTTTGGG CCTTTGGGAA ACACTCAGCA 
GCTGCAGACA TCACCTCTTC ACTGTCAGAG GACCAGGTCC CGGGGCCGTT TTTGGTGATG 
GTCCTCATTC AGTTTGGAAC CATGGTGGTG GACCGAGCCC TCTACCTCAG GAAGACTGTA 
CTGGGAAAGG TCATCTTCCA GGTCATTCTT GTGTTCGGAA TTCACTTCTG GATGTTCTTC 
ATCTTACCTG GTGTGACTGA GAGGAAATTC AGCCAGAACC TGGTTGCCCA GCTTTGGTAC 
TTTGTGAAAT GTGTTTACTT CGGGTTGTCT GCTTACCAGA TCCGTTGTGG CTACCCAACG 
CGAGTCCTGG GGAACTTCCT CACCAAGAGC TACAATTACG TCAACCTCTT CTTATTCCAA 
GGGTTTCGCC TCGTGCCCTT TTTGACTGAG CTGAGGGCAG TGATGGACTG GGTGTGGACG 
GACACAACTT TGAGCCTGTC CAGCTGGATC TGTGTGGAGG ACATCTATGC TCACATATTC 
ATCCTGAAGT GTTGGCGGGA GTCGGAGAAG AGATACCCTC AGCCACGGGG CCAGAAGAAG 
AAGAAAGTGG TGAAGTATGG CATGGGAGGA ATGATCATCG TCCTGCTCAT CTGCATTGTC 
TGGTTTCCTC TTCTCTTCAT GTCTTTGATC AAATCTGTGG CTGGGGTCAT CAACCAGCCC 
CTGGACGTCT CCGTCACAAT TACCCTGGGA GGGTATCAGC CTATTTTCAC AATGAGTGCC 
CAACAAAGCC AGTTGAAAGT TATGGACCAG CAGAGCTTTA ACAAATTTAT ACAAGCTTTT 
TCTAGGGACA CCGGTGCTAT GCAATTTCTG GAAAATTATG AAAAAGAAGA CATAACAGTA 
GCAGAACTGG AAGGAAACTC AAATTCTTTG TGGACCATCA GCCCACCCAG TAAGCAGAAA 
ATGATACACG AACTCCTGGA CCCCAATAGT AGCTTCTCTG TTGTTTTTTC ATGGAGTATT 
CAGAGAAACT TAAGTCTGGG TGCAAAATCG GAAATAGCAA CAGATAAGCT TTCTTTTCCT 
CTTAAAAATA TTACTCGAAA GAATATCGCT AAAATGATAG CAGGCAACAG CACAGAAAGT 
TCAAAAACAC CAGTGACCAT AGAAAAGATT TATCCATATT ATGTGAAAGC ACCTAGTGAT 
TCTAACTCAA AACCTATAAA GCAACTTTTA TCTGAAAATA ATTTCATGGA TATTACCATC 
ATTTTGTCCA GAGACAATAC AACTAAATAT AACAGTGAGT GGTGGGTTCT CAACCTGACT 
GGAAACAGAA TATACAATCC GAACTCTCAG GCCCTGGAAC TGGTGGTCTT CAATGACAAA 
GTCAGTCCCC CAAGTCTGGG GTTCCTGGCT GGCTATGGTA TTATGGGATT ATATGCTTCA 
GTTGTCCTTG TGATTGGGAA ATTTGTCCGT GAATTCTTCA GTGGGATTTC TCACTCCATC 
ATGTTTGAAG AGCTTCCAAA TGTGGATCGA ATTTTGAAGT TGTGCACAGA TATTTTTTTA 
GTTCGAGAGA CAGGAGAACT GGAGCTAGAA GAAGATCTCT ATGCCAAATT AATATTCCTA 
TATCGCTCAC CAGAGACAAT GATCAAATGG ACTAGAGAAA AAACAAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIEZO2 ( XM_011525723.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524025.1 Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X1, mRNA.

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CloneID	OHu71131
Clone ID Related Accession (Same CDS sequence)	XM_011525723.2 , XM_011525723.3
Accession Version	XM_011525723.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	piezo-type mechanosensitive ion channel component 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011525723.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG AAGTGGTGTG CGGGCTCATC TTCAGGCTGC TGCTGCCCAT CTGCCTGGCA 
GTAGCATGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC 
ATTCCTCTGT TCTCAGAACC AACAAAAACG ACGATGCAAG GACATACGGG ACGGTTATTA 
AAGTCTCTGT GCTTCATCAG TCTTTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG 
TTGGTGAGCC TTGAAGCTCA ACATCGTATT GCACCTGGCT ACAACTGCTC AACATGGGAA 
AAGACATTCC GGCAGATCGG CTTTGAAAGC TTAAAGGGAG CTGATGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCCTCTGT 
AGAAACATTG TTCAGAAACC TGTGACAGAC GAAGCAGCAC AGAGTAACCC GGAGTTTGAA 
AATGAAGAAT TGGCTGAAGG AGAAAAAATT GATTCAGAAG AGGCACTGAT CTATGAAGAG 
GATTTCAATG GAGGAGATGG TGTTGAAGGC GAGTTGGAAG AAAGCACGAA GTTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCCTCTAAG CTCAAGGAGT TCATTGGCAA CATGATCACC 
ACTGCTGGGA AAGTCGTTGT TACCATCTTA CTGGGCTCCT CGGGCATGAT GTTGCCGTCT 
TTGACATCAT CTGTGTATTT TTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACTGCT 
GGACATTTGA TTGGACTTTA TTTATACCAG TTCCAATTCT TTCAAGAGGC AGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAGTCAGTAA TTCAAACGGA CTGTTCAAGT 
ACTTGGAAGA TCATAGTGAA CCCGGACCTG TCGTGGTACC ACCACGCCAA CCCTATCCTC 
CTGCTGGTGA TGTACTACAC TCTGGCCACT CTGATCCGCA TCTGGCTGCA AGAGCCCCTT 
GTGCAGGATG AGGGGACCAA AGAAGAGGAC AAAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACAGCGGGGA GGAGGCGGAG CCTGTGGTAC GCAACCCATT ACCCCACTGA TGAGAGAAAA 
CTTTTATCCA TGACCCAGGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC 
GGCAACCCCG TGGATTACCA CACCATCCAC CCAAGCCTGC CCATGGAGAA CGGCCCTGGC 
AAAGCCGACC TCTACTCCAC CCCTCAGTAC CGGTGGGAGC CCTCTGATGA ATCCTCAGAA 
AAGCGAGAGG AGGAAGAGGA AGAGAAAGAA GAATTTGAAG AAGAAAGGAG CCGTGAGGAA 
AAAAGAAGTA TCAAAGTTCA TGCCATGGTC TCCGTATTCC AATTTATTAT GAAACAAAGT 
TACATCTGTG CCCTCATAGC TATGATGGCC TGGAGCATCA CCTATCACAG CTGGCTGACC 
TTCGTGCTGC TGATCTGGTC GTGCACTCTT TGGATGATTC GCAACAGAAG AAAATATGCC 
ATGATCAGCT CTCCCTTCAT GGTGGTTTAT GGAAACCTAT TGTTGATATT ACAGTATATA 
TGGAGTTTTG AACTTCCTGA AATTAAAAAA GTTCCAGGAT TTTTAGAAAA GAAAGAGCCA 
GGAGAACTTG CTTCCAAAAT CCTTTTCACC ATTACTTTTT GGCTACTGCT GAGGCAGCAC 
CTCACAGAGC AAAAAGCTCT GCAAGAAAAG GAAGCTCTTT TATCGGAAGT CAAAATTGGC 
AGTCAGGAAA ATGAAGAAAA AGATGAGGAA CTTCAAGATA TACAAGTGGA AGGAGAGCCC 
AAAGAGGAGG AAGAAGAGGA AGCGAAGGAA GAGAAGCAAG AGAGAAAGAA GGTAGAGCAA 
GAGGAAGCTG AAGAAGAAGA TGAGCAGGAC ATCATGAAAG TCCTGGGCAA TCTGGTGGTG 
GCCATGTTCA TCAAGTACTG GATCTACGTC TGCGGAGGCA TGTTCTTCTT CGTCAGCTTC 
GAGGGTAAAA TCGTAATGTA CAAAATCATC TACATGGTGC TGTTCCTGTT CTGTGTGGCC 
CTATACCAGG TGCACTATGA ATGGTGGAGG AAAATTCTAA AATATTTTTG GATGTCAGTG 
GTTATTTACA CTATGCTGGT GCTTATCTTT ATATACACAT ATCAGTTTGA GAACTTCCCA 
GGCCTGTGGC AAAATATGAC TGGACTGAAA AAAGAAAAGC TTGAGGATCT TGGCTTAAAG 
CAGTTTACTG TGGCTGAACT ATTCACTCGC ATATTCATCC CAACCTCCTT TCTGCTGGTG 
TGCATTTTAC ACCTGCACTA CTTCCATGAC CGGTTCCTTG AACTCACAGA CCTCAAGTCC 
ATTCCCAGCA AAGAAGACAA CACCATCTAC AGCCATGCCA AAGTCAATGG TCGCGTATAT 
CTAATAATAA ATAGGATCTC ACACAGTCTT CCCACGGTTG CCAATCGACA GTTCGCATCA 
GTAACTCTCA GCATCAAGAA AAAATTACCA ATCCACCAAA ATGAACTGGC CCACCCGGAA 
GGAAGCCTCC CGGACCTCAC CATGATGCAT CTGACTGCCA GCCTGGAGAA GCCGGAGGTG 
AGGAAGTTGG CTGAGCCTGG GGAGGAGAAG CTTGAGGGCT ACTCTGAAAA AGCCCAGAAG 
GGTGATCTTG GGAAAGACAG CGAGGAGTCA GAGGAGGACG GAGAGGAAGA GGAGGAATCC 
GAGGAGGAGG AAGAAACATC AGACTTAAGG AACAAATGGC ACCTGGTGAT TGACCGCCTC 
ACTGTGCTCT TCTTAAAATT CCTGGAGTAT TTTCACAAGC TGCAGGTGTT CATGTGGTGG 
ATTTTGGAGT TGCACATCAT CAAAATCGTT TCCTCTTACA TTATCTGGGT TTCTGTGAAA 
GAGGTGTCTC TGTTCAACTA TGTATTTTTG ATTTCTTGGG CTTTTGCTCT GCCGTACGCC 
AAGCTGCGCC GTCTGGCTTC AAGTGTCTGC ACAGTCTGGA CGTGTGTGAT CATCGTCTGC 
AAAATGTTGT ACCAGCTCCA AACCATTAAG CCTGAGAACT TCTCTGTTAA CTGTTCCTTG 
CCAAATGAAA ATCAAACAAA CATCCCCTTT AATGAGTTGA ACAAGTCTCT GCTCTACAGC 
GCTCCTATCG ATCCTACAGA GTGGGTCGGC CTGCGGAAGT CTTCGCCTCT GCTAGTCTAC 
CTGAGGAATA ACCTCCTGAT GCTGGCTATC CTGGCCTTTG AAGTCACCAT TTACCGCCAT 
CAGGAATACT ATCGAGGTCG AAATAACCTG ACGGCCCCTG TGTCTAGAAC TATCTTTCAT 
GACATTACAA GACTACATCT AGATGATGGA CTTATTAATT GTGCCAAATA TTTCATTAAT 
TACTTCTTTT ACAAGTTTGG TCTGGAGACC TGTTTCCTAA TGTCAGTTAA CGTCATTGGC 
CAGCGAATGG ATTTCTATGC CATGATCCAC GCCTGCTGGC TGATCGCTGT CTTATATAGA 
CGCAGAAGGA AAGCCATCGC AGAGATCTGG CCCAAGTACT GCTGCTTCCT GGCATGCATC 
ATCACCTTCC AGTATTTCAT CTGCATTGGC ATCCCACCTG CTCCTTGCCG AGATTACCCG 
TGGAGATTCA AGGGTGCCAG CTTCAATGAC AACATCATAA AGTGGCTGTA CTTCCCAGAT 
TTCATTGTGC GGCCCAACCC TGTGTTTCTC GTCTATGACT TCATGCTGCT TCTGTGTGCC 
TCCTTACAAC GGCAGATTTT TGAGGATGAG AACAAGGCTG CAGTGCGAAT CATGGCAGGT 
GACAATGTCG AGATCTGCAT GAACCTTGAT GCGGCCTCCT TCAGCCAACA TAACCCTGTG 
CCAGATTTTA TTCACTGCAG ATCTTACTTA GACATGTCCA AAGTGATCAT CTTCAGCTAC 
CTCTTCTGGT TTGTGCTCAC CATCATCTTC ATCACTGGGA CCACCAGGAT CAGCATCTTT 
TGCATGGGGT ACCTGGTGGC CTGTTTCTAC TTCCTGCTCT TTGGGGGCGA TTTGCTGTTG 
AAACCCATCA AGAGCATCCT GCGCTACTGG GACTGGCTGA TCGCATACAA CGTTTTTGTG 
ATTACGATGA AAAATATCCT GTCAATAGGA GCATGTGGAT ACATTGGAAC ATTGGTGCAC 
AATAGTTGTT GGTTGATCCA GGCTTTCAGC CTGGCCTGCA CAGTCAAAGG CTATCAAATG 
CCTGCTGCTA ATTCACCCTG TACACTTCCC AGTGGGGAAG CAGGAATCAT TTGGGACAGC 
ATATGTTTTG CCTTCCTCCT GCTGCAAAGA AGAGTTTTCA TGAGTTATTA TTTTCTACAT 
GTTGTGGCTG ATATAAAAGC TTCCCAGATT CTGGCATCAA GAGGAGCTGA ACTTTTCCAG 
GCCACAATTG TAAAAGCTGT AAAGGCAAGA ATTGAGGAAG AGAAGAAGTC CATGGACCAG 
CTGAAGCGAC AGATGGATCG CATCAAGGCC AGGCAACAGA AATATAAAAA GGGTAAGGAG 
AGGATGCTGA GCTTGACCCA GGAGCCAGGG GAAGGCCAGG ACATGCAAAA ACTCTCTGAA 
GAGGATGATG AAAGAGAAGC AGACAAACAG AAAGCCAAGG GCAAAAAAAA GCAGTGGTGG 
CGGCCTTGGG TTGATCATGC TTCCATGGTC AGGAGTGGAG ATTATTATTT GTTTGAAACG 
GATAGTGAAG AGGAGGAAGA GGAAGAATTA AAGAAGGAAG ATGAAGAACC TCCACGAAGG 
TCAGCATTCC AGTTTGTTTA TCAAGCCTGG ATTACTGATC CTAAAACAGC ACTCCGACAA 
AGACACAAAG AGAAAAAAAG GTCTGCAAGA GAAGAACGGA AACGAAGGCG GAAAGGATCC 
AAGGAGGGTC CTGTGGAATG GGAAGACCGG GAGGATGAAC CAATCAAAAA GAAATCCGAT 
GGACCAGATA ATATCATCAA GAGGATATTT AATATTTTGA AATTTACCTG GGTCCTATTT 
CTGGCAACAG TGGACAGTTT CACTACTTGG CTTAACTCCA TTTCAAGGGA GCATATTGAT 
ATATCTACAG TTCTGAGAAT TGAACGATGC ATGCTGACCA GAGAAATTAA GAAGGGCAAT 
GTTCCAACTC GGGAGAGCAT CCACATGTAC TATCAGAACC ACATCATGAA CCTTTCCAGA 
GAGTCGGGAC TGGACACCAT TGACGAGCAT CCCGGAGCTG CTTCAGGTGC ACAGACAGCC 
CACAGGATGG ATAGTTTAGA TTCACATGAC AGTATCTCCA GCGAGCCCAC GCAGTGTACC 
ATGCTGTACT CACGCCAGGG GACCACTGAG ACCATCGAGG AGGTGGAGGC TGAGCAGGAG 
GAGGAGGCAG GGAGCACGGC GCCTGAGCCC AGGGAGGCCA AGGAGTACGA GGCCACTGGG 
TACGATGTGG GAGCCATGGG TGCCGAGGAG GCCAGCCTCA CCCCAGAGGA AGAGCTGACA 
CAGTTCTCCA CCTTGGACGG GGATGTGGAG GCCCCACCCT CCTACAGCAA GGCTGTGAGC 
TTCGAGCATC TGTCCTTCGG CTCGCAGGAC GACTCTGCAG GCAAGAACCG TATGGCAGTC 
AGCCCGGACG ACAGCCGCAC CGACAAGCTG GGGTCCAGCA TCTTACCTCC CCTGACCCAT 
GAGCTGACGG CCAGCGAGCT GCTGCTGAAA AAGATGTTTC ACGACGATGA GCTTGAAGAG 
TCAGAGAAAT TCTACGTGGG GCAGCCCCGA TTTCTGCTGC TCTTCTATGC CATGTACAAT 
ACCCTGGTGG CCCGCTCGGA GATGGTGTGC TACTTCGTGA TCATCCTCAA CCACATGGTC 
TCTGCCTCCA TGATCACGCT CCTGCTTCCC ATCCTCATCT TCCTCTGGGC CATGTTGTCC 
GTCCCCAGGC CCAGCCGCCG GTTCTGGATG ATGGCCATCG TCTATACTGA GGTGGCAATT 
GTAGTCAAGT ATTTCTTCCA ATTTGGGTTC TTTCCCTGGA ATAAGAATGT GGAGGTGAAC 
AAAGATAAAC CGTATCACCC CCCAAACATC ATAGGAGTGG AAAAGAAGGA AGGTTATGTT 
CTCTATGACC TCATCCAGCT CCTGGCTCTG TTCTTTCATC GATCAATTTT GAAGTGCCAT 
GGCTTATGGG ATGAAGATGA CATGACTGAA AGTGGCATGG CCAGGGAGGA ATCAGATGAT 
GAGCTCTCCC TCGGTCATGG CAGGAGGGAC TCCTCCGATT CTCTCAAGTC CATCAACCTG 
GCCGCGTCTG TGGAGTCAGT GCATGTGACC TTCCCGGAGC AGCAGACAGC TGTCCGGAGG 
AAGCGCTCCG GCAGCAGCTC CGAGCCATCC CAGAGATCCA GCTTTTCTTC AAACAGATCC 
CAAAGAGGCA GCACAAGCAC CCGAAACAGC AGTCAAAAAG GAAGCAGTGT TTTGAGTATT 
AAGCAAAAAG GCAAAAGGGA ACTTTATATG GAAAAGCTTC AAGAACATTT AATCAAAGCA 
AAAGCCTTTA CCATAAAGAA GACGCTGGAG ATCTATGTGC CCATCAAACA GTTCTTTTAC 
AACCTCATCC ACCCGGAGTA TAGCGCCGTG ACTGACGTGT ATGTACTCAT GTTCCTGGCT 
GACACTGTGG ACTTCATCAT CATTGTCTTC GGCTTTTGGG CCTTTGGGAA ACACTCAGCA 
GCTGCAGACA TCACCTCTTC ACTGTCAGAG GACCAGGTCC CGGGGCCGTT TTTGGTGATG 
GTCCTCATTC AGTTTGGAAC CATGGTGGTG GACCGAGCCC TCTACCTCAG GAAGACTGTA 
CTGGGAAAGG TCATCTTCCA GGTCATTCTT GTGTTCGGAA TTCACTTCTG GATGTTCTTC 
ATCTTACCTG GTGTGACTGA GAGGAAATTC AGCCAGAACC TGGTTGCCCA GCTTTGGTAC 
TTTGTGAAAT GTGTTTACTT CGGGTTGTCT GCTTACCAGA TCCGTTGTGG CTACCCAACG 
CGAGTCCTGG GGAACTTCCT CACCAAGAGC TACAATTACG TCAACCTCTT CTTATTCCAA 
GGGTTTCGCC TCGTGCCCTT TTTGACTGAG CTGAGGGCAG TGATGGACTG GGTGTGGACG 
GACACAACTT TGAGCCTGTC CAGCTGGATC TGTGTGGAGG ACATCTATGC TCACATATTC 
ATCCTGAAGT GTTGGCGGGA GTCGGAGAAG AGATACCCTC AGCCACGGGG CCAGAAGAAG 
AAGAAAGTGG TGAAGTATGG CATGGGAGGA ATGATCATCG TCCTGCTCAT CTGCATTGTC 
TGGTTTCCTC TTCTCTTCAT GTCTTTGATC AAATCTGTGG CTGGGGTCAT CAACCAGCCC 
CTGGACGTCT CCGTCACAAT TACCCTGGGA GGGTATCAGC CTATTTTCAC AATGAGTGCC 
CAACAAAGCC AGTTGAAAGT TATGGACCAG CAGAGCTTTA ACAAATTTAT ACAAGCTTTT 
TCTAGGGACA CCGGTGCTAT GCAATTTCTG GAAAATTATG AAAAAGAAGA CATAACAGTA 
GCAGAACTGG AAGGAAACTC AAATTCTTTG TGGACCATCA GCCCACCCAG TAAGCAGAAA 
ATGATACACG AACTCCTGGA CCCCAATAGT AGCTTCTCTG TTGTTTTTTC ATGGAGTATT 
CAGAGAAACT TAAGTCTGGG TGCAAAATCG GAAATAGCAA CAGATAAGCT TTCTTTTCCT 
CTTAAAAATA TTACTCGAAA GAATATCGCT AAAATGATAG CAGGCAACAG CACAGAAAGT 
TCAAAAACAC CAGTGACCAT AGAAAAGATT TATCCATATT ATGTGAAAGC ACCTAGTGAT 
TCTAACTCAA AACCTATAAA GCAACTTTTA TCTGAAAATA ATTTCATGGA TATTACCATC 
ATTTTGTCCA GAGACAATAC AACTAAATAT AACAGTGAGT GGTGGGTTCT CAACCTGACT 
GGAAACAGAA TATACAATCC GAACTCTCAG GCCCTGGAAC TGGTGGTCTT CAATGACAAA 
GTCAGTCCCC CAAGTCTGGG GTTCCTGGCT GGCTATGGTA TTATGGGATT ATATGCTTCA 
GTTGTCCTTG TGATTGGGAA ATTTGTCCGT GAATTCTTCA GTGGGATTTC TCACTCCATC 
ATGTTTGAAG AGCTTCCAAA TGTGGATCGA ATTTTGAAGT TGTGCACAGA TATTTTTTTA 
GTTCGAGAGA CAGGAGAACT GGAGCTAGAA GAAGATCTCT ATGCCAAATT AATATTCCTA 
TATCGCTCAC CAGAGACAAT GATCAAATGG ACTAGAGAAA AAACAAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524025.1
CDS	998..9388
Translation	MASEVVCGLIFRLLLPICLAVACAFRYNGLSFVYLIYLLLIPLFSEPTKTTMQGHTGRLLKSLCFISLSFLLLHIIFHITLVSLEAQHRIAPGYNCSTWEKTFRQIGFESLKGADAGNGIRVFVPDIGMFIASLTIWLLCRNIVQKPVTDEAAQSNPEFENEELAEGEKIDSEEALIYEEDFNGGDGVEGELEESTKLKMFRRLASVASKLKEFIGNMITTAGKVVVTILLGSSGMMLPSLTSSVYFFVFLGLCTWWSWCRTFDPLLFSCLCVLLAIFTAGHLIGLYLYQFQFFQEAVPPNDYYARLFGIKSVIQTDCSSTWKIIVNPDLSWYHHANPILLLVMYYTLATLIRIWLQEPLVQDEGTKEEDKALACSPIQITAGRRRSLWYATHYPTDERKLLSMTQDDYKPSDGLLVTVNGNPVDYHTIHPSLPMENGPGKADLYSTPQYRWEPSDESSEKREEEEEEKEEFEEERSREEKRSIKVHAMVSVFQFIMKQSYICALIAMMAWSITYHSWLTFVLLIWSCTLWMIRNRRKYAMISSPFMVVYGNLLLILQYIWSFELPEIKKVPGFLEKKEPGELASKILFTITFWLLLRQHLTEQKALQEKEALLSEVKIGSQENEEKDEELQDIQVEGEPKEEEEEEAKEEKQERKKVEQEEAEEEDEQDIMKVLGNLVVAMFIKYWIYVCGGMFFFVSFEGKIVMYKIIYMVLFLFCVALYQVHYEWWRKILKYFWMSVVIYTMLVLIFIYTYQFENFPGLWQNMTGLKKEKLEDLGLKQFTVAELFTRIFIPTSFLLVCILHLHYFHDRFLELTDLKSIPSKEDNTIYSHAKVNGRVYLIINRISHSLPTVANRQFASVTLSIKKKLPIHQNELAHPEGSLPDLTMMHLTASLEKPEVRKLAEPGEEKLEGYSEKAQKGDLGKDSEESEEDGEEEEESEEEEETSDLRNKWHLVIDRLTVLFLKFLEYFHKLQVFMWWILELHIIKIVSSYIIWVSVKEVSLFNYVFLISWAFALPYAKLRRLASSVCTVWTCVIIVCKMLYQLQTIKPENFSVNCSLPNENQTNIPFNELNKSLLYSAPIDPTEWVGLRKSSPLLVYLRNNLLMLAILAFEVTIYRHQEYYRGRNNLTAPVSRTIFHDITRLHLDDGLINCAKYFINYFFYKFGLETCFLMSVNVIGQRMDFYAMIHACWLIAVLYRRRRKAIAEIWPKYCCFLACIITFQYFICIGIPPAPCRDYPWRFKGASFNDNIIKWLYFPDFIVRPNPVFLVYDFMLLLCASLQRQIFEDENKAAVRIMAGDNVEICMNLDAASFSQHNPVPDFIHCRSYLDMSKVIIFSYLFWFVLTIIFITGTTRISIFCMGYLVACFYFLLFGGDLLLKPIKSILRYWDWLIAYNVFVITMKNILSIGACGYIGTLVHNSCWLIQAFSLACTVKGYQMPAANSPCTLPSGEAGIIWDSICFAFLLLQRRVFMSYYFLHVVADIKASQILASRGAELFQATIVKAVKARIEEEKKSMDQLKRQMDRIKARQQKYKKGKERMLSLTQEPGEGQDMQKLSEEDDEREADKQKAKGKKKQWWRPWVDHASMVRSGDYYLFETDSEEEEEEELKKEDEEPPRRSAFQFVYQAWITDPKTALRQRHKEKKRSAREERKRRRKGSKEGPVEWEDREDEPIKKKSDGPDNIIKRIFNILKFTWVLFLATVDSFTTWLNSISREHIDISTVLRIERCMLTREIKKGNVPTRESIHMYYQNHIMNLSRESGLDTIDEHPGAASGAQTAHRMDSLDSHDSISSEPTQCTMLYSRQGTTETIEEVEAEQEEEAGSTAPEPREAKEYEATGYDVGAMGAEEASLTPEEELTQFSTLDGDVEAPPSYSKAVSFEHLSFGSQDDSAGKNRMAVSPDDSRTDKLGSSILPPLTHELTASELLLKKMFHDDELEESEKFYVGQPRFLLLFYAMYNTLVARSEMVCYFVIILNHMVSASMITLLLPILIFLWAMLSVPRPSRRFWMMAIVYTEVAIVVKYFFQFGFFPWNKNVEVNKDKPYHPPNIIGVEKKEGYVLYDLIQLLALFFHRSILKCHGLWDEDDMTESGMAREESDDELSLGHGRRDSSDSLKSINLAASVESVHVTFPEQQTAVRRKRSGSSSEPSQRSSFSSNRSQRGSTSTRNSSQKGSSVLSIKQKGKRELYMEKLQEHLIKAKAFTIKKTLEIYVPIKQFFYNLIHPEYSAVTDVYVLMFLADTVDFIIIVFGFWAFGKHSAAADITSSLSEDQVPGPFLVMVLIQFGTMVVDRALYLRKTVLGKVIFQVILVFGIHFWMFFILPGVTERKFSQNLVAQLWYFVKCVYFGLSAYQIRCGYPTRVLGNFLTKSYNYVNLFLFQGFRLVPFLTELRAVMDWVWTDTTLSLSSWICVEDIYAHIFILKCWRESEKRYPQPRGQKKKKVVKYGMGGMIIVLLICIVWFPLLFMSLIKSVAGVINQPLDVSVTITLGGYQPIFTMSAQQSQLKVMDQQSFNKFIQAFSRDTGAMQFLENYEKEDITVAELEGNSNSLWTISPPSKQKMIHELLDPNSSFSVVFSWSIQRNLSLGAKSEIATDKLSFPLKNITRKNIAKMIAGNSTESSKTPVTIEKIYPYYVKAPSDSNSKPIKQLLSENNFMDITIILSRDNTTKYNSEWWVLNLTGNRIYNPNSQALELVVFNDKVSPPSLGFLAGYGIMGLYASVVLVIGKFVREFFSGISHSIMFEELPNVDRILKLCTDIFLVRETGELELEEDLYAKLIFLYRSPETMIKWTREKTN

Target ORF information:

RefSeq Version	XM_011525723.3
Organism	Homo sapiens(human)
Definition	Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525723.3

ORF Insert Sequence:

ATGGCCTCAG AAGTGGTGTG CGGGCTCATC TTCAGGCTGC TGCTGCCCAT CTGCCTGGCA 
GTAGCATGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC 
ATTCCTCTGT TCTCAGAACC AACAAAAACG ACGATGCAAG GACATACGGG ACGGTTATTA 
AAGTCTCTGT GCTTCATCAG TCTTTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG 
TTGGTGAGCC TTGAAGCTCA ACATCGTATT GCACCTGGCT ACAACTGCTC AACATGGGAA 
AAGACATTCC GGCAGATCGG CTTTGAAAGC TTAAAGGGAG CTGATGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCCTCTGT 
AGAAACATTG TTCAGAAACC TGTGACAGAC GAAGCAGCAC AGAGTAACCC GGAGTTTGAA 
AATGAAGAAT TGGCTGAAGG AGAAAAAATT GATTCAGAAG AGGCACTGAT CTATGAAGAG 
GATTTCAATG GAGGAGATGG TGTTGAAGGC GAGTTGGAAG AAAGCACGAA GTTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCCTCTAAG CTCAAGGAGT TCATTGGCAA CATGATCACC 
ACTGCTGGGA AAGTCGTTGT TACCATCTTA CTGGGCTCCT CGGGCATGAT GTTGCCGTCT 
TTGACATCAT CTGTGTATTT TTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACTGCT 
GGACATTTGA TTGGACTTTA TTTATACCAG TTCCAATTCT TTCAAGAGGC AGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAGTCAGTAA TTCAAACGGA CTGTTCAAGT 
ACTTGGAAGA TCATAGTGAA CCCGGACCTG TCGTGGTACC ACCACGCCAA CCCTATCCTC 
CTGCTGGTGA TGTACTACAC TCTGGCCACT CTGATCCGCA TCTGGCTGCA AGAGCCCCTT 
GTGCAGGATG AGGGGACCAA AGAAGAGGAC AAAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACAGCGGGGA GGAGGCGGAG CCTGTGGTAC GCAACCCATT ACCCCACTGA TGAGAGAAAA 
CTTTTATCCA TGACCCAGGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC 
GGCAACCCCG TGGATTACCA CACCATCCAC CCAAGCCTGC CCATGGAGAA CGGCCCTGGC 
AAAGCCGACC TCTACTCCAC CCCTCAGTAC CGGTGGGAGC CCTCTGATGA ATCCTCAGAA 
AAGCGAGAGG AGGAAGAGGA AGAGAAAGAA GAATTTGAAG AAGAAAGGAG CCGTGAGGAA 
AAAAGAAGTA TCAAAGTTCA TGCCATGGTC TCCGTATTCC AATTTATTAT GAAACAAAGT 
TACATCTGTG CCCTCATAGC TATGATGGCC TGGAGCATCA CCTATCACAG CTGGCTGACC 
TTCGTGCTGC TGATCTGGTC GTGCACTCTT TGGATGATTC GCAACAGAAG AAAATATGCC 
ATGATCAGCT CTCCCTTCAT GGTGGTTTAT GGAAACCTAT TGTTGATATT ACAGTATATA 
TGGAGTTTTG AACTTCCTGA AATTAAAAAA GTTCCAGGAT TTTTAGAAAA GAAAGAGCCA 
GGAGAACTTG CTTCCAAAAT CCTTTTCACC ATTACTTTTT GGCTACTGCT GAGGCAGCAC 
CTCACAGAGC AAAAAGCTCT GCAAGAAAAG GAAGCTCTTT TATCGGAAGT CAAAATTGGC 
AGTCAGGAAA ATGAAGAAAA AGATGAGGAA CTTCAAGATA TACAAGTGGA AGGAGAGCCC 
AAAGAGGAGG AAGAAGAGGA AGCGAAGGAA GAGAAGCAAG AGAGAAAGAA GGTAGAGCAA 
GAGGAAGCTG AAGAAGAAGA TGAGCAGGAC ATCATGAAAG TCCTGGGCAA TCTGGTGGTG 
GCCATGTTCA TCAAGTACTG GATCTACGTC TGCGGAGGCA TGTTCTTCTT CGTCAGCTTC 
GAGGGTAAAA TCGTAATGTA CAAAATCATC TACATGGTGC TGTTCCTGTT CTGTGTGGCC 
CTATACCAGG TGCACTATGA ATGGTGGAGG AAAATTCTAA AATATTTTTG GATGTCAGTG 
GTTATTTACA CTATGCTGGT GCTTATCTTT ATATACACAT ATCAGTTTGA GAACTTCCCA 
GGCCTGTGGC AAAATATGAC TGGACTGAAA AAAGAAAAGC TTGAGGATCT TGGCTTAAAG 
CAGTTTACTG TGGCTGAACT ATTCACTCGC ATATTCATCC CAACCTCCTT TCTGCTGGTG 
TGCATTTTAC ACCTGCACTA CTTCCATGAC CGGTTCCTTG AACTCACAGA CCTCAAGTCC 
ATTCCCAGCA AAGAAGACAA CACCATCTAC AGCCATGCCA AAGTCAATGG TCGCGTATAT 
CTAATAATAA ATAGGATCTC ACACAGTCTT CCCACGGTTG CCAATCGACA GTTCGCATCA 
GTAACTCTCA GCATCAAGAA AAAATTACCA ATCCACCAAA ATGAACTGGC CCACCCGGAA 
GGAAGCCTCC CGGACCTCAC CATGATGCAT CTGACTGCCA GCCTGGAGAA GCCGGAGGTG 
AGGAAGTTGG CTGAGCCTGG GGAGGAGAAG CTTGAGGGCT ACTCTGAAAA AGCCCAGAAG 
GGTGATCTTG GGAAAGACAG CGAGGAGTCA GAGGAGGACG GAGAGGAAGA GGAGGAATCC 
GAGGAGGAGG AAGAAACATC AGACTTAAGG AACAAATGGC ACCTGGTGAT TGACCGCCTC 
ACTGTGCTCT TCTTAAAATT CCTGGAGTAT TTTCACAAGC TGCAGGTGTT CATGTGGTGG 
ATTTTGGAGT TGCACATCAT CAAAATCGTT TCCTCTTACA TTATCTGGGT TTCTGTGAAA 
GAGGTGTCTC TGTTCAACTA TGTATTTTTG ATTTCTTGGG CTTTTGCTCT GCCGTACGCC 
AAGCTGCGCC GTCTGGCTTC AAGTGTCTGC ACAGTCTGGA CGTGTGTGAT CATCGTCTGC 
AAAATGTTGT ACCAGCTCCA AACCATTAAG CCTGAGAACT TCTCTGTTAA CTGTTCCTTG 
CCAAATGAAA ATCAAACAAA CATCCCCTTT AATGAGTTGA ACAAGTCTCT GCTCTACAGC 
GCTCCTATCG ATCCTACAGA GTGGGTCGGC CTGCGGAAGT CTTCGCCTCT GCTAGTCTAC 
CTGAGGAATA ACCTCCTGAT GCTGGCTATC CTGGCCTTTG AAGTCACCAT TTACCGCCAT 
CAGGAATACT ATCGAGGTCG AAATAACCTG ACGGCCCCTG TGTCTAGAAC TATCTTTCAT 
GACATTACAA GACTACATCT AGATGATGGA CTTATTAATT GTGCCAAATA TTTCATTAAT 
TACTTCTTTT ACAAGTTTGG TCTGGAGACC TGTTTCCTAA TGTCAGTTAA CGTCATTGGC 
CAGCGAATGG ATTTCTATGC CATGATCCAC GCCTGCTGGC TGATCGCTGT CTTATATAGA 
CGCAGAAGGA AAGCCATCGC AGAGATCTGG CCCAAGTACT GCTGCTTCCT GGCATGCATC 
ATCACCTTCC AGTATTTCAT CTGCATTGGC ATCCCACCTG CTCCTTGCCG AGATTACCCG 
TGGAGATTCA AGGGTGCCAG CTTCAATGAC AACATCATAA AGTGGCTGTA CTTCCCAGAT 
TTCATTGTGC GGCCCAACCC TGTGTTTCTC GTCTATGACT TCATGCTGCT TCTGTGTGCC 
TCCTTACAAC GGCAGATTTT TGAGGATGAG AACAAGGCTG CAGTGCGAAT CATGGCAGGT 
GACAATGTCG AGATCTGCAT GAACCTTGAT GCGGCCTCCT TCAGCCAACA TAACCCTGTG 
CCAGATTTTA TTCACTGCAG ATCTTACTTA GACATGTCCA AAGTGATCAT CTTCAGCTAC 
CTCTTCTGGT TTGTGCTCAC CATCATCTTC ATCACTGGGA CCACCAGGAT CAGCATCTTT 
TGCATGGGGT ACCTGGTGGC CTGTTTCTAC TTCCTGCTCT TTGGGGGCGA TTTGCTGTTG 
AAACCCATCA AGAGCATCCT GCGCTACTGG GACTGGCTGA TCGCATACAA CGTTTTTGTG 
ATTACGATGA AAAATATCCT GTCAATAGGA GCATGTGGAT ACATTGGAAC ATTGGTGCAC 
AATAGTTGTT GGTTGATCCA GGCTTTCAGC CTGGCCTGCA CAGTCAAAGG CTATCAAATG 
CCTGCTGCTA ATTCACCCTG TACACTTCCC AGTGGGGAAG CAGGAATCAT TTGGGACAGC 
ATATGTTTTG CCTTCCTCCT GCTGCAAAGA AGAGTTTTCA TGAGTTATTA TTTTCTACAT 
GTTGTGGCTG ATATAAAAGC TTCCCAGATT CTGGCATCAA GAGGAGCTGA ACTTTTCCAG 
GCCACAATTG TAAAAGCTGT AAAGGCAAGA ATTGAGGAAG AGAAGAAGTC CATGGACCAG 
CTGAAGCGAC AGATGGATCG CATCAAGGCC AGGCAACAGA AATATAAAAA GGGTAAGGAG 
AGGATGCTGA GCTTGACCCA GGAGCCAGGG GAAGGCCAGG ACATGCAAAA ACTCTCTGAA 
GAGGATGATG AAAGAGAAGC AGACAAACAG AAAGCCAAGG GCAAAAAAAA GCAGTGGTGG 
CGGCCTTGGG TTGATCATGC TTCCATGGTC AGGAGTGGAG ATTATTATTT GTTTGAAACG 
GATAGTGAAG AGGAGGAAGA GGAAGAATTA AAGAAGGAAG ATGAAGAACC TCCACGAAGG 
TCAGCATTCC AGTTTGTTTA TCAAGCCTGG ATTACTGATC CTAAAACAGC ACTCCGACAA 
AGACACAAAG AGAAAAAAAG GTCTGCAAGA GAAGAACGGA AACGAAGGCG GAAAGGATCC 
AAGGAGGGTC CTGTGGAATG GGAAGACCGG GAGGATGAAC CAATCAAAAA GAAATCCGAT 
GGACCAGATA ATATCATCAA GAGGATATTT AATATTTTGA AATTTACCTG GGTCCTATTT 
CTGGCAACAG TGGACAGTTT CACTACTTGG CTTAACTCCA TTTCAAGGGA GCATATTGAT 
ATATCTACAG TTCTGAGAAT TGAACGATGC ATGCTGACCA GAGAAATTAA GAAGGGCAAT 
GTTCCAACTC GGGAGAGCAT CCACATGTAC TATCAGAACC ACATCATGAA CCTTTCCAGA 
GAGTCGGGAC TGGACACCAT TGACGAGCAT CCCGGAGCTG CTTCAGGTGC ACAGACAGCC 
CACAGGATGG ATAGTTTAGA TTCACATGAC AGTATCTCCA GCGAGCCCAC GCAGTGTACC 
ATGCTGTACT CACGCCAGGG GACCACTGAG ACCATCGAGG AGGTGGAGGC TGAGCAGGAG 
GAGGAGGCAG GGAGCACGGC GCCTGAGCCC AGGGAGGCCA AGGAGTACGA GGCCACTGGG 
TACGATGTGG GAGCCATGGG TGCCGAGGAG GCCAGCCTCA CCCCAGAGGA AGAGCTGACA 
CAGTTCTCCA CCTTGGACGG GGATGTGGAG GCCCCACCCT CCTACAGCAA GGCTGTGAGC 
TTCGAGCATC TGTCCTTCGG CTCGCAGGAC GACTCTGCAG GCAAGAACCG TATGGCAGTC 
AGCCCGGACG ACAGCCGCAC CGACAAGCTG GGGTCCAGCA TCTTACCTCC CCTGACCCAT 
GAGCTGACGG CCAGCGAGCT GCTGCTGAAA AAGATGTTTC ACGACGATGA GCTTGAAGAG 
TCAGAGAAAT TCTACGTGGG GCAGCCCCGA TTTCTGCTGC TCTTCTATGC CATGTACAAT 
ACCCTGGTGG CCCGCTCGGA GATGGTGTGC TACTTCGTGA TCATCCTCAA CCACATGGTC 
TCTGCCTCCA TGATCACGCT CCTGCTTCCC ATCCTCATCT TCCTCTGGGC CATGTTGTCC 
GTCCCCAGGC CCAGCCGCCG GTTCTGGATG ATGGCCATCG TCTATACTGA GGTGGCAATT 
GTAGTCAAGT ATTTCTTCCA ATTTGGGTTC TTTCCCTGGA ATAAGAATGT GGAGGTGAAC 
AAAGATAAAC CGTATCACCC CCCAAACATC ATAGGAGTGG AAAAGAAGGA AGGTTATGTT 
CTCTATGACC TCATCCAGCT CCTGGCTCTG TTCTTTCATC GATCAATTTT GAAGTGCCAT 
GGCTTATGGG ATGAAGATGA CATGACTGAA AGTGGCATGG CCAGGGAGGA ATCAGATGAT 
GAGCTCTCCC TCGGTCATGG CAGGAGGGAC TCCTCCGATT CTCTCAAGTC CATCAACCTG 
GCCGCGTCTG TGGAGTCAGT GCATGTGACC TTCCCGGAGC AGCAGACAGC TGTCCGGAGG 
AAGCGCTCCG GCAGCAGCTC CGAGCCATCC CAGAGATCCA GCTTTTCTTC AAACAGATCC 
CAAAGAGGCA GCACAAGCAC CCGAAACAGC AGTCAAAAAG GAAGCAGTGT TTTGAGTATT 
AAGCAAAAAG GCAAAAGGGA ACTTTATATG GAAAAGCTTC AAGAACATTT AATCAAAGCA 
AAAGCCTTTA CCATAAAGAA GACGCTGGAG ATCTATGTGC CCATCAAACA GTTCTTTTAC 
AACCTCATCC ACCCGGAGTA TAGCGCCGTG ACTGACGTGT ATGTACTCAT GTTCCTGGCT 
GACACTGTGG ACTTCATCAT CATTGTCTTC GGCTTTTGGG CCTTTGGGAA ACACTCAGCA 
GCTGCAGACA TCACCTCTTC ACTGTCAGAG GACCAGGTCC CGGGGCCGTT TTTGGTGATG 
GTCCTCATTC AGTTTGGAAC CATGGTGGTG GACCGAGCCC TCTACCTCAG GAAGACTGTA 
CTGGGAAAGG TCATCTTCCA GGTCATTCTT GTGTTCGGAA TTCACTTCTG GATGTTCTTC 
ATCTTACCTG GTGTGACTGA GAGGAAATTC AGCCAGAACC TGGTTGCCCA GCTTTGGTAC 
TTTGTGAAAT GTGTTTACTT CGGGTTGTCT GCTTACCAGA TCCGTTGTGG CTACCCAACG 
CGAGTCCTGG GGAACTTCCT CACCAAGAGC TACAATTACG TCAACCTCTT CTTATTCCAA 
GGGTTTCGCC TCGTGCCCTT TTTGACTGAG CTGAGGGCAG TGATGGACTG GGTGTGGACG 
GACACAACTT TGAGCCTGTC CAGCTGGATC TGTGTGGAGG ACATCTATGC TCACATATTC 
ATCCTGAAGT GTTGGCGGGA GTCGGAGAAG AGATACCCTC AGCCACGGGG CCAGAAGAAG 
AAGAAAGTGG TGAAGTATGG CATGGGAGGA ATGATCATCG TCCTGCTCAT CTGCATTGTC 
TGGTTTCCTC TTCTCTTCAT GTCTTTGATC AAATCTGTGG CTGGGGTCAT CAACCAGCCC 
CTGGACGTCT CCGTCACAAT TACCCTGGGA GGGTATCAGC CTATTTTCAC AATGAGTGCC 
CAACAAAGCC AGTTGAAAGT TATGGACCAG CAGAGCTTTA ACAAATTTAT ACAAGCTTTT 
TCTAGGGACA CCGGTGCTAT GCAATTTCTG GAAAATTATG AAAAAGAAGA CATAACAGTA 
GCAGAACTGG AAGGAAACTC AAATTCTTTG TGGACCATCA GCCCACCCAG TAAGCAGAAA 
ATGATACACG AACTCCTGGA CCCCAATAGT AGCTTCTCTG TTGTTTTTTC ATGGAGTATT 
CAGAGAAACT TAAGTCTGGG TGCAAAATCG GAAATAGCAA CAGATAAGCT TTCTTTTCCT 
CTTAAAAATA TTACTCGAAA GAATATCGCT AAAATGATAG CAGGCAACAG CACAGAAAGT 
TCAAAAACAC CAGTGACCAT AGAAAAGATT TATCCATATT ATGTGAAAGC ACCTAGTGAT 
TCTAACTCAA AACCTATAAA GCAACTTTTA TCTGAAAATA ATTTCATGGA TATTACCATC 
ATTTTGTCCA GAGACAATAC AACTAAATAT AACAGTGAGT GGTGGGTTCT CAACCTGACT 
GGAAACAGAA TATACAATCC GAACTCTCAG GCCCTGGAAC TGGTGGTCTT CAATGACAAA 
GTCAGTCCCC CAAGTCTGGG GTTCCTGGCT GGCTATGGTA TTATGGGATT ATATGCTTCA 
GTTGTCCTTG TGATTGGGAA ATTTGTCCGT GAATTCTTCA GTGGGATTTC TCACTCCATC 
ATGTTTGAAG AGCTTCCAAA TGTGGATCGA ATTTTGAAGT TGTGCACAGA TATTTTTTTA 
GTTCGAGAGA CAGGAGAACT GGAGCTAGAA GAAGATCTCT ATGCCAAATT AATATTCCTA 
TATCGCTCAC CAGAGACAAT GATCAAATGG ACTAGAGAAA AAACAAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIEZO2 ( XM_011525724.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524026.1 Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X3, mRNA.

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CloneID	OHu71132
Clone ID Related Accession (Same CDS sequence)	XM_011525724.3 , XM_011525724.2
Accession Version	XM_011525724.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	piezo-type mechanosensitive ion channel component 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011525724.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG AAGTGGTGTG CGGGCTCATC TTCAGGCTGC TGCTGCCCAT CTGCCTGGCA 
GTAGCATGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC 
ATTCCTCTGT TCTCAGAACC AACAAAAACG ACGATGCAAG GACATACGGG ACGGTTATTA 
AAGTCTCTGT GCTTCATCAG TCTTTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG 
TTGGTGAGCC TTGAAGCTCA ACATCGTATT GCACCTGGCT ACAACTGCTC AACATGGGAA 
AAGACATTCC GGCAGATCGG CTTTGAAAGC TTAAAGGGAG CTGATGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCCTCTGT 
AGAAACATTG TTCAGAAACC TGTGACAGAC GAAGCAGCAC AGAGTAACCC GGAGTTTGAA 
AATGAAGAAT TGGCTGAAGG AGAAAAAATT GATTCAGAAG AGGCACTGAT CTATGAAGAG 
GATTTCAATG GAGGAGATGG TGTTGAAGGC GAGTTGGAAG AAAGCACGAA GTTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCCTCTAAG CTCAAGGAGT TCATTGGCAA CATGATCACC 
ACTGCTGGGA AAGTCGTTGT TACCATCTTA CTGGGCTCCT CGGGCATGAT GTTGCCGTCT 
TTGACATCAT CTGTGTATTT TTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACTGCT 
GGACATTTGA TTGGACTTTA TTTATACCAG TTCCAATTCT TTCAAGAGGC AGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAGTCAGTAA TTCAAACGGA CTGTTCAAGT 
ACTTGGAAGA TCATAGTGAA CCCGGACCTG TCGTGGTACC ACCACGCCAA CCCTATCCTC 
CTGCTGGTGA TGTACTACAC TCTGGCCACT CTGATCCGCA TCTGGCTGCA AGAGCCCCTT 
GTGCAGGATG AGGGGACCAA AGAAGAGGAC AAAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACAGCGGGGA GGAGGCGGAG CCTGTGGTAC GCAACCCATT ACCCCACTGA TGAGAGAAAA 
CTTTTATCCA TGACCCAGGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC 
GGCAACCCCG TGGATTACCA CACCATCCAC CCAAGCCTGC CCATGGAGAA CGGCCCTGGC 
AAAGCCGACC TCTACTCCAC CCCTCAGTAC CGGTGGGAGC CCTCTGATGA ATCCTCAGAA 
AAGCGAGAGG AGGAAGAGGA AGAGAAAGAA GAATTTGAAG AAGAAAGGAG CCGTGAGGAA 
AAAAGAAGTA TCAAAGTTCA TGCCATGGTC TCCGTATTCC AATTTATTAT GAAACAAAGT 
TACATCTGTG CCCTCATAGC TATGATGGCC TGGAGCATCA CCTATCACAG CTGGCTGACC 
TTCGTGCTGC TGATCTGGTC GTGCACTCTT TGGATGATTC GCAACAGAAG AAAATATGCC 
ATGATCAGCT CTCCCTTCAT GGTGGTTTAT GGAAACCTAT TGTTGATATT ACAGTATATA 
TGGAGTTTTG AACTTCCTGA AATTAAAAAA GTTCCAGGAT TTTTAGAAAA GAAAGAGCCA 
GGAGAACTTG CTTCCAAAAT CCTTTTCACC ATTACTTTTT GGCTACTGCT GAGGCAGCAC 
CTCACAGAGC AAAAAGCTCT GCAAGAAAAG GAAGCTCTTT TATCGGAAGT CAAAATTGGC 
AGTCAGGAAA ATGAAGAAAA AGATGAGGAA CTTCAAGATA TACAAGTGGA AGGAGAGCCC 
AAAGAGGAGG AAGAAGAGGA AGCGAAGGAA GAGAAGCAAG AGAGAAAGAA GGTAGAGCAA 
GAGGAAGCTG AAGAAGAAGA TGAGCAGGAC ATCATGAAAG TCCTGGGCAA TCTGGTGGTG 
GCCATGTTCA TCAAGTACTG GATCTACGTC TGCGGAGGCA TGTTCTTCTT CGTCAGCTTC 
GAGGGTAAAA TCGTAATGTA CAAAATCATC TACATGGTGC TGTTCCTGTT CTGTGTGGCC 
CTATACCAGG TGCACTATGA ATGGTGGAGG AAAATTCTAA AATATTTTTG GATGTCAGTG 
GTTATTTACA CTATGCTGGT GCTTATCTTT ATATACACAT ATCAGTTTGA GAACTTCCCA 
GGCCTGTGGC AAAATATGAC TGGACTGAAA AAAGAAAAGC TTGAGGATCT TGGCTTAAAG 
CAGTTTACTG TGGCTGAACT ATTCACTCGC ATATTCATCC CAACCTCCTT TCTGCTGGTG 
TGCATTTTAC ACCTGCACTA CTTCCATGAC CGGTTCCTTG AACTCACAGA CCTCAAGTCC 
ATTCCCAGCA AAGAAGACAA CACCATCTAC AGCCATGCCA AAGTCAATGG TCGCGTATAT 
CTAATAATAA ATAGCATCAA GAAAAAATTA CCAATCCACC AAAATGAACT GGCCCACCCG 
GAAGGAAGCC TCCCGGACCT CACCATGATG CATCTGACTG CCAGCCTGGA GAAGCCGGAG 
GTGAGGAAGT TGGCTGAGCC TGGGGAGGAG AAGCTTGAGG GCTACTCTGA AAAAGCCCAG 
AAGGGTGATC TTGGGAAAGA CAGCGAGGAG TCAGAGGAGG ACGGAGAGGA AGAGGAGGAA 
TCCGAGGAGG AGGAAGAAAC ATCAGACTTA AGGAACAAAT GGCACCTGGT GATTGACCGC 
CTCACTGTGC TCTTCTTAAA ATTCCTGGAG TATTTTCACA AGCTGCAGGT GTTCATGTGG 
TGGATTTTGG AGTTGCACAT CATCAAAATC GTTTCCTCTT ACATTATCTG GGTTTCTGTG 
AAAGAGGTGT CTCTGTTCAA CTATGTATTT TTGATTTCTT GGGCTTTTGC TCTGCCGTAC 
GCCAAGCTGC GCCGTCTGGC TTCAAGTGTC TGCACAGTCT GGACGTGTGT GATCATCGTC 
TGCAAAATGT TGTACCAGCT CCAAACCATT AAGCCTGAGA ACTTCTCTGT TAACTGTTCC 
TTGCCAAATG AAAATCAAAC AAACATCCCC TTTAATGAGT TGAACAAGTC TCTGCTCTAC 
AGCGCTCCTA TCGATCCTAC AGAGTGGGTC GGCCTGCGGA AGTCTTCGCC TCTGCTAGTC 
TACCTGAGGA ATAACCTCCT GATGCTGGCT ATCCTGGCCT TTGAAGTCAC CATTTACCGC 
CATCAGGAAT ACTATCGAGG TCGAAATAAC CTGACGGCCC CTGTGTCTAG AACTATCTTT 
CATGACATTA CAAGACTACA TCTAGATGAT GGACTTATTA ATTGTGCCAA ATATTTCATT 
AATTACTTCT TTTACAAGTT TGGTCTGGAG ACCTGTTTCC TAATGTCAGT TAACGTCATT 
GGCCAGCGAA TGGATTTCTA TGCCATGATC CACGCCTGCT GGCTGATCGC TGTCTTATAT 
AGACGCAGAA GGAAAGCCAT CGCAGAGATC TGGCCCAAGT ACTGCTGCTT CCTGGCATGC 
ATCATCACCT TCCAGTATTT CATCTGCATT GGCATCCCAC CTGCTCCTTG CCGAGATTAC 
CCGTGGAGAT TCAAGGGTGC CAGCTTCAAT GACAACATCA TAAAGTGGCT GTACTTCCCA 
GATTTCATTG TGCGGCCCAA CCCTGTGTTT CTCGTCTATG ACTTCATGCT GCTTCTGTGT 
GCCTCCTTAC AACGGCAGAT TTTTGAGGAT GAGAACAAGG CTGCAGTGCG AATCATGGCA 
GGTGACAATG TCGAGATCTG CATGAACCTT GATGCGGCCT CCTTCAGCCA ACATAACCCT 
GTGCCAGATT TTATTCACTG CAGATCTTAC TTAGACATGT CCAAAGTGAT CATCTTCAGC 
TACCTCTTCT GGTTTGTGCT CACCATCATC TTCATCACTG GGACCACCAG GATCAGCATC 
TTTTGCATGG GGTACCTGGT GGCCTGTTTC TACTTCCTGC TCTTTGGGGG CGATTTGCTG 
TTGAAACCCA TCAAGAGCAT CCTGCGCTAC TGGGACTGGC TGATCGCATA CAACGTTTTT 
GTGATTACGA TGAAAAATAT CCTGTCAATA GGAGCATGTG GATACATTGG AACATTGGTG 
CACAATAGTT GTTGGTTGAT CCAGGCTTTC AGCCTGGCCT GCACAGTCAA AGGCTATCAA 
ATGCCTGCTG CTAATTCACC CTGTACACTT CCCAGTGGGG AAGCAGGAAT CATTTGGGAC 
AGCATATGTT TTGCCTTCCT CCTGCTGCAA AGAAGAGTTT TCATGAGTTA TTATTTTCTA 
CATGTTGTGG CTGATATAAA AGCTTCCCAG ATTCTGGCAT CAAGAGGAGC TGAACTTTTC 
CAGGCCACAA TTGTAAAAGC TGTAAAGGCA AGAATTGAGG AAGAGAAGAA GTCCATGGAC 
CAGCTGAAGC GACAGATGGA TCGCATCAAG GCCAGGCAAC AGAAATATAA AAAGGGTAAG 
GAGAGGATGC TGAGCTTGAC CCAGGAGCCA GGGGAAGGCC AGGACATGCA AAAACTCTCT 
GAAGAGGATG ATGAAAGAGA AGCAGACAAA CAGAAAGCCA AGGGCAAAAA AAAGCAGTGG 
TGGCGGCCTT GGGTTGATCA TGCTTCCATG GTCAGGAGTG GAGATTATTA TTTGTTTGAA 
ACGGATAGTG AAGAGGAGGA AGAGGAAGAA TTAAAGAAGG AAGATGAAGA ACCTCCACGA 
AGGTCAGCAT TCCAGTTTGT TTATCAAGCC TGGATTACTG ATCCTAAAAC AGCACTCCGA 
CAAAGACACA AAGAGAAAAA AAGGTCTGCA AGAGAAGAAC GGAAACGAAG GCGGAAAGGA 
TCCAAGGAGG GTCCTGTGGA ATGGGAAGAC CGGGAGGATG AACCAATCAA AAAGAAATCC 
GATGGACCAG ATAATATCAT CAAGAGGATA TTTAATATTT TGAAATTTAC CTGGGTCCTA 
TTTCTGGCAA CAGTGGACAG TTTCACTACT TGGCTTAACT CCATTTCAAG GGAGCATATT 
GATATATCTA CAGTTCTGAG AATTGAACGA TGCATGCTGA CCAGAGAAAT TAAGAAGGGC 
AATGTTCCAA CTCGGGAGAG CATCCACATG TACTATCAGA ACCACATCAT GAACCTTTCC 
AGAGAGTCGG GACTGGACAC CATTGACGAG CATCCCGGAG CTGCTTCAGG TGCACAGACA 
GCCCACAGGA TGGATAGTTT AGATTCACAT GACAGTATCT CCAGCGAGCC CACGCAGTGT 
ACCATGCTGT ACTCACGCCA GGGGACCACT GAGACCATCG AGGAGGTGGA GGCTGAGCAG 
GAGGAGGAGG CAGGGAGCAC GGCGCCTGAG CCCAGGGAGG CCAAGGAGTA CGAGGCCACT 
GGGTACGATG TGGGAGCCAT GGGTGCCGAG GAGGCCAGCC TCACCCCAGA GGAAGAGCTG 
ACACAGTTCT CCACCTTGGA CGGGGATGTG GAGGCCCCAC CCTCCTACAG CAAGGCTGTG 
AGCTTCGAGC ATCTGTCCTT CGGCTCGCAG GACGACTCTG CAGGCAAGAA CCGTATGGCA 
GTCAGCCCGG ACGACAGCCG CACCGACAAG CTGGGGTCCA GCATCTTACC TCCCCTGACC 
CATGAGCTGA CGGCCAGCGA GCTGCTGCTG AAAAAGATGT TTCACGACGA TGAGCTTGAA 
GAGTCAGAGA AATTCTACGT GGGGCAGCCC CGATTTCTGC TGCTCTTCTA TGCCATGTAC 
AATACCCTGG TGGCCCGCTC GGAGATGGTG TGCTACTTCG TGATCATCCT CAACCACATG 
GTCTCTGCCT CCATGATCAC GCTCCTGCTT CCCATCCTCA TCTTCCTCTG GGCCATGTTG 
TCCGTCCCCA GGCCCAGCCG CCGGTTCTGG ATGATGGCCA TCGTCTATAC TGAGGTGGCA 
ATTGTAGTCA AGTATTTCTT CCAATTTGGG TTCTTTCCCT GGAATAAGAA TGTGGAGGTG 
AACAAAGATA AACCGTATCA CCCCCCAAAC ATCATAGGAG TGGAAAAGAA GGAAGGTTAT 
GTTCTCTATG ACCTCATCCA GCTCCTGGCT CTGTTCTTTC ATCGATCAAT TTTGAAGTGC 
CATGGCTTAT GGGATGAAGA TGACATGACT GAAAGTGGCA TGGCCAGGGA GGAATCAGAT 
GATGAGCTCT CCCTCGGTCA TGGCAGGAGG GACTCCTCCG ATTCTCTCAA GTCCATCAAC 
CTGGCCGCGT CTGTGGAGTC AGTGCATGTG ACCTTCCCGG AGCAGCAGAC AGCTGTCCGG 
AGGAAGCGCT CCGGCAGCAG CTCCGAGCCA TCCCAGAGAT CCAGCTTTTC TTCAAACAGA 
TCCCAAAGAG GCAGCACAAG CACCCGAAAC AGCAGTCAAA AAGGAAGCAG TGTTTTGAGT 
ATTAAGCAAA AAGGCAAAAG GGAACTTTAT ATGGAAAAGC TTCAAGAACA TTTAATCAAA 
GCAAAAGCCT TTACCATAAA GAAGACGCTG GAGATCTATG TGCCCATCAA ACAGTTCTTT 
TACAACCTCA TCCACCCGGA GTATAGCGCC GTGACTGACG TGTATGTACT CATGTTCCTG 
GCTGACACTG TGGACTTCAT CATCATTGTC TTCGGCTTTT GGGCCTTTGG GAAACACTCA 
GCAGCTGCAG ACATCACCTC TTCACTGTCA GAGGACCAGG TCCCGGGGCC GTTTTTGGTG 
ATGGTCCTCA TTCAGTTTGG AACCATGGTG GTGGACCGAG CCCTCTACCT CAGGAAGACT 
GTACTGGGAA AGGTCATCTT CCAGGTCATT CTTGTGTTCG GAATTCACTT CTGGATGTTC 
TTCATCTTAC CTGGTGTGAC TGAGAGGAAA TTCAGCCAGA ACCTGGTTGC CCAGCTTTGG 
TACTTTGTGA AATGTGTTTA CTTCGGGTTG TCTGCTTACC AGATCCGTTG TGGCTACCCA 
ACGCGAGTCC TGGGGAACTT CCTCACCAAG AGCTACAATT ACGTCAACCT CTTCTTATTC 
CAAGGGTTTC GCCTCGTGCC CTTTTTGACT GAGCTGAGGG CAGTGATGGA CTGGGTGTGG 
ACGGACACAA CTTTGAGCCT GTCCAGCTGG ATCTGTGTGG AGGACATCTA TGCTCACATA 
TTCATCCTGA AGTGTTGGCG GGAGTCGGAG AAGAGATACC CTCAGCCACG GGGCCAGAAG 
AAGAAGAAAG TGGTGAAGTA TGGCATGGGA GGAATGATCA TCGTCCTGCT CATCTGCATT 
GTCTGGTTTC CTCTTCTCTT CATGTCTTTG ATCAAATCTG TGGCTGGGGT CATCAACCAG 
CCCCTGGACG TCTCCGTCAC AATTACCCTG GGAGGGTATC AGCCTATTTT CACAATGAGT 
GCCCAACAAA GCCAGTTGAA AGTTATGGAC CAGCAGAGCT TTAACAAATT TATACAAGCT 
TTTTCTAGGG ACACCGGTGC TATGCAATTT CTGGAAAATT ATGAAAAAGA AGACATAACA 
GTAGCAGAAC TGGAAGGAAA CTCAAATTCT TTGTGGACCA TCAGCCCACC CAGTAAGCAG 
AAAATGATAC ACGAACTCCT GGACCCCAAT AGTAGCTTCT CTGTTGTTTT TTCATGGAGT 
ATTCAGAGAA ACTTAAGTCT GGGTGCAAAA TCGGAAATAG CAACAGATAA GCTTTCTTTT 
CCTCTTAAAA ATATTACTCG AAAGAATATC GCTAAAATGA TAGCAGGCAA CAGCACAGAA 
AGTTCAAAAA CACCAGTGAC CATAGAAAAG ATTTATCCAT ATTATGTGAA AGCACCTAGT 
GATTCTAACT CAAAACCTAT AAAGCAACTT TTATCTGAAA ATAATTTCAT GGATATTACC 
ATCATTTTGT CCAGAGACAA TACAACTAAA TATAACAGTG AGTGGTGGGT TCTCAACCTG 
ACTGGAAACA GAATATACAA TCCGAACTCT CAGGCCCTGG AACTGGTGGT CTTCAATGAC 
AAAGTCAGTC CCCCAAGTCT GGGGTTCCTG GCTGGCTATG GTATTATGGG ATTATATGCT 
TCAGTTGTCC TTGTGATTGG GAAATTTGTC CGTGAATTCT TCAGTGGGAT TTCTCACTCC 
ATCATGTTTG AAGAGCTTCC AAATGTGGAT CGAATTTTGA AGTTGTGCAC AGATATTTTT 
TTAGTTCGAG AGACAGGAGA ACTGGAGCTA GAAGAAGATC TCTATGCCAA ATTAATATTC 
CTATATCGCT CACCAGAGAC AATGATCAAA TGGACTAGAG AAAAAACAAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524026.1
CDS	998..9331
Translation	MASEVVCGLIFRLLLPICLAVACAFRYNGLSFVYLIYLLLIPLFSEPTKTTMQGHTGRLLKSLCFISLSFLLLHIIFHITLVSLEAQHRIAPGYNCSTWEKTFRQIGFESLKGADAGNGIRVFVPDIGMFIASLTIWLLCRNIVQKPVTDEAAQSNPEFENEELAEGEKIDSEEALIYEEDFNGGDGVEGELEESTKLKMFRRLASVASKLKEFIGNMITTAGKVVVTILLGSSGMMLPSLTSSVYFFVFLGLCTWWSWCRTFDPLLFSCLCVLLAIFTAGHLIGLYLYQFQFFQEAVPPNDYYARLFGIKSVIQTDCSSTWKIIVNPDLSWYHHANPILLLVMYYTLATLIRIWLQEPLVQDEGTKEEDKALACSPIQITAGRRRSLWYATHYPTDERKLLSMTQDDYKPSDGLLVTVNGNPVDYHTIHPSLPMENGPGKADLYSTPQYRWEPSDESSEKREEEEEEKEEFEEERSREEKRSIKVHAMVSVFQFIMKQSYICALIAMMAWSITYHSWLTFVLLIWSCTLWMIRNRRKYAMISSPFMVVYGNLLLILQYIWSFELPEIKKVPGFLEKKEPGELASKILFTITFWLLLRQHLTEQKALQEKEALLSEVKIGSQENEEKDEELQDIQVEGEPKEEEEEEAKEEKQERKKVEQEEAEEEDEQDIMKVLGNLVVAMFIKYWIYVCGGMFFFVSFEGKIVMYKIIYMVLFLFCVALYQVHYEWWRKILKYFWMSVVIYTMLVLIFIYTYQFENFPGLWQNMTGLKKEKLEDLGLKQFTVAELFTRIFIPTSFLLVCILHLHYFHDRFLELTDLKSIPSKEDNTIYSHAKVNGRVYLIINSIKKKLPIHQNELAHPEGSLPDLTMMHLTASLEKPEVRKLAEPGEEKLEGYSEKAQKGDLGKDSEESEEDGEEEEESEEEEETSDLRNKWHLVIDRLTVLFLKFLEYFHKLQVFMWWILELHIIKIVSSYIIWVSVKEVSLFNYVFLISWAFALPYAKLRRLASSVCTVWTCVIIVCKMLYQLQTIKPENFSVNCSLPNENQTNIPFNELNKSLLYSAPIDPTEWVGLRKSSPLLVYLRNNLLMLAILAFEVTIYRHQEYYRGRNNLTAPVSRTIFHDITRLHLDDGLINCAKYFINYFFYKFGLETCFLMSVNVIGQRMDFYAMIHACWLIAVLYRRRRKAIAEIWPKYCCFLACIITFQYFICIGIPPAPCRDYPWRFKGASFNDNIIKWLYFPDFIVRPNPVFLVYDFMLLLCASLQRQIFEDENKAAVRIMAGDNVEICMNLDAASFSQHNPVPDFIHCRSYLDMSKVIIFSYLFWFVLTIIFITGTTRISIFCMGYLVACFYFLLFGGDLLLKPIKSILRYWDWLIAYNVFVITMKNILSIGACGYIGTLVHNSCWLIQAFSLACTVKGYQMPAANSPCTLPSGEAGIIWDSICFAFLLLQRRVFMSYYFLHVVADIKASQILASRGAELFQATIVKAVKARIEEEKKSMDQLKRQMDRIKARQQKYKKGKERMLSLTQEPGEGQDMQKLSEEDDEREADKQKAKGKKKQWWRPWVDHASMVRSGDYYLFETDSEEEEEEELKKEDEEPPRRSAFQFVYQAWITDPKTALRQRHKEKKRSAREERKRRRKGSKEGPVEWEDREDEPIKKKSDGPDNIIKRIFNILKFTWVLFLATVDSFTTWLNSISREHIDISTVLRIERCMLTREIKKGNVPTRESIHMYYQNHIMNLSRESGLDTIDEHPGAASGAQTAHRMDSLDSHDSISSEPTQCTMLYSRQGTTETIEEVEAEQEEEAGSTAPEPREAKEYEATGYDVGAMGAEEASLTPEEELTQFSTLDGDVEAPPSYSKAVSFEHLSFGSQDDSAGKNRMAVSPDDSRTDKLGSSILPPLTHELTASELLLKKMFHDDELEESEKFYVGQPRFLLLFYAMYNTLVARSEMVCYFVIILNHMVSASMITLLLPILIFLWAMLSVPRPSRRFWMMAIVYTEVAIVVKYFFQFGFFPWNKNVEVNKDKPYHPPNIIGVEKKEGYVLYDLIQLLALFFHRSILKCHGLWDEDDMTESGMAREESDDELSLGHGRRDSSDSLKSINLAASVESVHVTFPEQQTAVRRKRSGSSSEPSQRSSFSSNRSQRGSTSTRNSSQKGSSVLSIKQKGKRELYMEKLQEHLIKAKAFTIKKTLEIYVPIKQFFYNLIHPEYSAVTDVYVLMFLADTVDFIIIVFGFWAFGKHSAAADITSSLSEDQVPGPFLVMVLIQFGTMVVDRALYLRKTVLGKVIFQVILVFGIHFWMFFILPGVTERKFSQNLVAQLWYFVKCVYFGLSAYQIRCGYPTRVLGNFLTKSYNYVNLFLFQGFRLVPFLTELRAVMDWVWTDTTLSLSSWICVEDIYAHIFILKCWRESEKRYPQPRGQKKKKVVKYGMGGMIIVLLICIVWFPLLFMSLIKSVAGVINQPLDVSVTITLGGYQPIFTMSAQQSQLKVMDQQSFNKFIQAFSRDTGAMQFLENYEKEDITVAELEGNSNSLWTISPPSKQKMIHELLDPNSSFSVVFSWSIQRNLSLGAKSEIATDKLSFPLKNITRKNIAKMIAGNSTESSKTPVTIEKIYPYYVKAPSDSNSKPIKQLLSENNFMDITIILSRDNTTKYNSEWWVLNLTGNRIYNPNSQALELVVFNDKVSPPSLGFLAGYGIMGLYASVVLVIGKFVREFFSGISHSIMFEELPNVDRILKLCTDIFLVRETGELELEEDLYAKLIFLYRSPETMIKWTREKTN

Target ORF information:

RefSeq Version	XM_011525724.3
Organism	Homo sapiens(human)
Definition	Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525724.3

ORF Insert Sequence:

ATGGCCTCAG AAGTGGTGTG CGGGCTCATC TTCAGGCTGC TGCTGCCCAT CTGCCTGGCA 
GTAGCATGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC 
ATTCCTCTGT TCTCAGAACC AACAAAAACG ACGATGCAAG GACATACGGG ACGGTTATTA 
AAGTCTCTGT GCTTCATCAG TCTTTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG 
TTGGTGAGCC TTGAAGCTCA ACATCGTATT GCACCTGGCT ACAACTGCTC AACATGGGAA 
AAGACATTCC GGCAGATCGG CTTTGAAAGC TTAAAGGGAG CTGATGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCCTCTGT 
AGAAACATTG TTCAGAAACC TGTGACAGAC GAAGCAGCAC AGAGTAACCC GGAGTTTGAA 
AATGAAGAAT TGGCTGAAGG AGAAAAAATT GATTCAGAAG AGGCACTGAT CTATGAAGAG 
GATTTCAATG GAGGAGATGG TGTTGAAGGC GAGTTGGAAG AAAGCACGAA GTTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCCTCTAAG CTCAAGGAGT TCATTGGCAA CATGATCACC 
ACTGCTGGGA AAGTCGTTGT TACCATCTTA CTGGGCTCCT CGGGCATGAT GTTGCCGTCT 
TTGACATCAT CTGTGTATTT TTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACTGCT 
GGACATTTGA TTGGACTTTA TTTATACCAG TTCCAATTCT TTCAAGAGGC AGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAGTCAGTAA TTCAAACGGA CTGTTCAAGT 
ACTTGGAAGA TCATAGTGAA CCCGGACCTG TCGTGGTACC ACCACGCCAA CCCTATCCTC 
CTGCTGGTGA TGTACTACAC TCTGGCCACT CTGATCCGCA TCTGGCTGCA AGAGCCCCTT 
GTGCAGGATG AGGGGACCAA AGAAGAGGAC AAAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACAGCGGGGA GGAGGCGGAG CCTGTGGTAC GCAACCCATT ACCCCACTGA TGAGAGAAAA 
CTTTTATCCA TGACCCAGGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC 
GGCAACCCCG TGGATTACCA CACCATCCAC CCAAGCCTGC CCATGGAGAA CGGCCCTGGC 
AAAGCCGACC TCTACTCCAC CCCTCAGTAC CGGTGGGAGC CCTCTGATGA ATCCTCAGAA 
AAGCGAGAGG AGGAAGAGGA AGAGAAAGAA GAATTTGAAG AAGAAAGGAG CCGTGAGGAA 
AAAAGAAGTA TCAAAGTTCA TGCCATGGTC TCCGTATTCC AATTTATTAT GAAACAAAGT 
TACATCTGTG CCCTCATAGC TATGATGGCC TGGAGCATCA CCTATCACAG CTGGCTGACC 
TTCGTGCTGC TGATCTGGTC GTGCACTCTT TGGATGATTC GCAACAGAAG AAAATATGCC 
ATGATCAGCT CTCCCTTCAT GGTGGTTTAT GGAAACCTAT TGTTGATATT ACAGTATATA 
TGGAGTTTTG AACTTCCTGA AATTAAAAAA GTTCCAGGAT TTTTAGAAAA GAAAGAGCCA 
GGAGAACTTG CTTCCAAAAT CCTTTTCACC ATTACTTTTT GGCTACTGCT GAGGCAGCAC 
CTCACAGAGC AAAAAGCTCT GCAAGAAAAG GAAGCTCTTT TATCGGAAGT CAAAATTGGC 
AGTCAGGAAA ATGAAGAAAA AGATGAGGAA CTTCAAGATA TACAAGTGGA AGGAGAGCCC 
AAAGAGGAGG AAGAAGAGGA AGCGAAGGAA GAGAAGCAAG AGAGAAAGAA GGTAGAGCAA 
GAGGAAGCTG AAGAAGAAGA TGAGCAGGAC ATCATGAAAG TCCTGGGCAA TCTGGTGGTG 
GCCATGTTCA TCAAGTACTG GATCTACGTC TGCGGAGGCA TGTTCTTCTT CGTCAGCTTC 
GAGGGTAAAA TCGTAATGTA CAAAATCATC TACATGGTGC TGTTCCTGTT CTGTGTGGCC 
CTATACCAGG TGCACTATGA ATGGTGGAGG AAAATTCTAA AATATTTTTG GATGTCAGTG 
GTTATTTACA CTATGCTGGT GCTTATCTTT ATATACACAT ATCAGTTTGA GAACTTCCCA 
GGCCTGTGGC AAAATATGAC TGGACTGAAA AAAGAAAAGC TTGAGGATCT TGGCTTAAAG 
CAGTTTACTG TGGCTGAACT ATTCACTCGC ATATTCATCC CAACCTCCTT TCTGCTGGTG 
TGCATTTTAC ACCTGCACTA CTTCCATGAC CGGTTCCTTG AACTCACAGA CCTCAAGTCC 
ATTCCCAGCA AAGAAGACAA CACCATCTAC AGCCATGCCA AAGTCAATGG TCGCGTATAT 
CTAATAATAA ATAGCATCAA GAAAAAATTA CCAATCCACC AAAATGAACT GGCCCACCCG 
GAAGGAAGCC TCCCGGACCT CACCATGATG CATCTGACTG CCAGCCTGGA GAAGCCGGAG 
GTGAGGAAGT TGGCTGAGCC TGGGGAGGAG AAGCTTGAGG GCTACTCTGA AAAAGCCCAG 
AAGGGTGATC TTGGGAAAGA CAGCGAGGAG TCAGAGGAGG ACGGAGAGGA AGAGGAGGAA 
TCCGAGGAGG AGGAAGAAAC ATCAGACTTA AGGAACAAAT GGCACCTGGT GATTGACCGC 
CTCACTGTGC TCTTCTTAAA ATTCCTGGAG TATTTTCACA AGCTGCAGGT GTTCATGTGG 
TGGATTTTGG AGTTGCACAT CATCAAAATC GTTTCCTCTT ACATTATCTG GGTTTCTGTG 
AAAGAGGTGT CTCTGTTCAA CTATGTATTT TTGATTTCTT GGGCTTTTGC TCTGCCGTAC 
GCCAAGCTGC GCCGTCTGGC TTCAAGTGTC TGCACAGTCT GGACGTGTGT GATCATCGTC 
TGCAAAATGT TGTACCAGCT CCAAACCATT AAGCCTGAGA ACTTCTCTGT TAACTGTTCC 
TTGCCAAATG AAAATCAAAC AAACATCCCC TTTAATGAGT TGAACAAGTC TCTGCTCTAC 
AGCGCTCCTA TCGATCCTAC AGAGTGGGTC GGCCTGCGGA AGTCTTCGCC TCTGCTAGTC 
TACCTGAGGA ATAACCTCCT GATGCTGGCT ATCCTGGCCT TTGAAGTCAC CATTTACCGC 
CATCAGGAAT ACTATCGAGG TCGAAATAAC CTGACGGCCC CTGTGTCTAG AACTATCTTT 
CATGACATTA CAAGACTACA TCTAGATGAT GGACTTATTA ATTGTGCCAA ATATTTCATT 
AATTACTTCT TTTACAAGTT TGGTCTGGAG ACCTGTTTCC TAATGTCAGT TAACGTCATT 
GGCCAGCGAA TGGATTTCTA TGCCATGATC CACGCCTGCT GGCTGATCGC TGTCTTATAT 
AGACGCAGAA GGAAAGCCAT CGCAGAGATC TGGCCCAAGT ACTGCTGCTT CCTGGCATGC 
ATCATCACCT TCCAGTATTT CATCTGCATT GGCATCCCAC CTGCTCCTTG CCGAGATTAC 
CCGTGGAGAT TCAAGGGTGC CAGCTTCAAT GACAACATCA TAAAGTGGCT GTACTTCCCA 
GATTTCATTG TGCGGCCCAA CCCTGTGTTT CTCGTCTATG ACTTCATGCT GCTTCTGTGT 
GCCTCCTTAC AACGGCAGAT TTTTGAGGAT GAGAACAAGG CTGCAGTGCG AATCATGGCA 
GGTGACAATG TCGAGATCTG CATGAACCTT GATGCGGCCT CCTTCAGCCA ACATAACCCT 
GTGCCAGATT TTATTCACTG CAGATCTTAC TTAGACATGT CCAAAGTGAT CATCTTCAGC 
TACCTCTTCT GGTTTGTGCT CACCATCATC TTCATCACTG GGACCACCAG GATCAGCATC 
TTTTGCATGG GGTACCTGGT GGCCTGTTTC TACTTCCTGC TCTTTGGGGG CGATTTGCTG 
TTGAAACCCA TCAAGAGCAT CCTGCGCTAC TGGGACTGGC TGATCGCATA CAACGTTTTT 
GTGATTACGA TGAAAAATAT CCTGTCAATA GGAGCATGTG GATACATTGG AACATTGGTG 
CACAATAGTT GTTGGTTGAT CCAGGCTTTC AGCCTGGCCT GCACAGTCAA AGGCTATCAA 
ATGCCTGCTG CTAATTCACC CTGTACACTT CCCAGTGGGG AAGCAGGAAT CATTTGGGAC 
AGCATATGTT TTGCCTTCCT CCTGCTGCAA AGAAGAGTTT TCATGAGTTA TTATTTTCTA 
CATGTTGTGG CTGATATAAA AGCTTCCCAG ATTCTGGCAT CAAGAGGAGC TGAACTTTTC 
CAGGCCACAA TTGTAAAAGC TGTAAAGGCA AGAATTGAGG AAGAGAAGAA GTCCATGGAC 
CAGCTGAAGC GACAGATGGA TCGCATCAAG GCCAGGCAAC AGAAATATAA AAAGGGTAAG 
GAGAGGATGC TGAGCTTGAC CCAGGAGCCA GGGGAAGGCC AGGACATGCA AAAACTCTCT 
GAAGAGGATG ATGAAAGAGA AGCAGACAAA CAGAAAGCCA AGGGCAAAAA AAAGCAGTGG 
TGGCGGCCTT GGGTTGATCA TGCTTCCATG GTCAGGAGTG GAGATTATTA TTTGTTTGAA 
ACGGATAGTG AAGAGGAGGA AGAGGAAGAA TTAAAGAAGG AAGATGAAGA ACCTCCACGA 
AGGTCAGCAT TCCAGTTTGT TTATCAAGCC TGGATTACTG ATCCTAAAAC AGCACTCCGA 
CAAAGACACA AAGAGAAAAA AAGGTCTGCA AGAGAAGAAC GGAAACGAAG GCGGAAAGGA 
TCCAAGGAGG GTCCTGTGGA ATGGGAAGAC CGGGAGGATG AACCAATCAA AAAGAAATCC 
GATGGACCAG ATAATATCAT CAAGAGGATA TTTAATATTT TGAAATTTAC CTGGGTCCTA 
TTTCTGGCAA CAGTGGACAG TTTCACTACT TGGCTTAACT CCATTTCAAG GGAGCATATT 
GATATATCTA CAGTTCTGAG AATTGAACGA TGCATGCTGA CCAGAGAAAT TAAGAAGGGC 
AATGTTCCAA CTCGGGAGAG CATCCACATG TACTATCAGA ACCACATCAT GAACCTTTCC 
AGAGAGTCGG GACTGGACAC CATTGACGAG CATCCCGGAG CTGCTTCAGG TGCACAGACA 
GCCCACAGGA TGGATAGTTT AGATTCACAT GACAGTATCT CCAGCGAGCC CACGCAGTGT 
ACCATGCTGT ACTCACGCCA GGGGACCACT GAGACCATCG AGGAGGTGGA GGCTGAGCAG 
GAGGAGGAGG CAGGGAGCAC GGCGCCTGAG CCCAGGGAGG CCAAGGAGTA CGAGGCCACT 
GGGTACGATG TGGGAGCCAT GGGTGCCGAG GAGGCCAGCC TCACCCCAGA GGAAGAGCTG 
ACACAGTTCT CCACCTTGGA CGGGGATGTG GAGGCCCCAC CCTCCTACAG CAAGGCTGTG 
AGCTTCGAGC ATCTGTCCTT CGGCTCGCAG GACGACTCTG CAGGCAAGAA CCGTATGGCA 
GTCAGCCCGG ACGACAGCCG CACCGACAAG CTGGGGTCCA GCATCTTACC TCCCCTGACC 
CATGAGCTGA CGGCCAGCGA GCTGCTGCTG AAAAAGATGT TTCACGACGA TGAGCTTGAA 
GAGTCAGAGA AATTCTACGT GGGGCAGCCC CGATTTCTGC TGCTCTTCTA TGCCATGTAC 
AATACCCTGG TGGCCCGCTC GGAGATGGTG TGCTACTTCG TGATCATCCT CAACCACATG 
GTCTCTGCCT CCATGATCAC GCTCCTGCTT CCCATCCTCA TCTTCCTCTG GGCCATGTTG 
TCCGTCCCCA GGCCCAGCCG CCGGTTCTGG ATGATGGCCA TCGTCTATAC TGAGGTGGCA 
ATTGTAGTCA AGTATTTCTT CCAATTTGGG TTCTTTCCCT GGAATAAGAA TGTGGAGGTG 
AACAAAGATA AACCGTATCA CCCCCCAAAC ATCATAGGAG TGGAAAAGAA GGAAGGTTAT 
GTTCTCTATG ACCTCATCCA GCTCCTGGCT CTGTTCTTTC ATCGATCAAT TTTGAAGTGC 
CATGGCTTAT GGGATGAAGA TGACATGACT GAAAGTGGCA TGGCCAGGGA GGAATCAGAT 
GATGAGCTCT CCCTCGGTCA TGGCAGGAGG GACTCCTCCG ATTCTCTCAA GTCCATCAAC 
CTGGCCGCGT CTGTGGAGTC AGTGCATGTG ACCTTCCCGG AGCAGCAGAC AGCTGTCCGG 
AGGAAGCGCT CCGGCAGCAG CTCCGAGCCA TCCCAGAGAT CCAGCTTTTC TTCAAACAGA 
TCCCAAAGAG GCAGCACAAG CACCCGAAAC AGCAGTCAAA AAGGAAGCAG TGTTTTGAGT 
ATTAAGCAAA AAGGCAAAAG GGAACTTTAT ATGGAAAAGC TTCAAGAACA TTTAATCAAA 
GCAAAAGCCT TTACCATAAA GAAGACGCTG GAGATCTATG TGCCCATCAA ACAGTTCTTT 
TACAACCTCA TCCACCCGGA GTATAGCGCC GTGACTGACG TGTATGTACT CATGTTCCTG 
GCTGACACTG TGGACTTCAT CATCATTGTC TTCGGCTTTT GGGCCTTTGG GAAACACTCA 
GCAGCTGCAG ACATCACCTC TTCACTGTCA GAGGACCAGG TCCCGGGGCC GTTTTTGGTG 
ATGGTCCTCA TTCAGTTTGG AACCATGGTG GTGGACCGAG CCCTCTACCT CAGGAAGACT 
GTACTGGGAA AGGTCATCTT CCAGGTCATT CTTGTGTTCG GAATTCACTT CTGGATGTTC 
TTCATCTTAC CTGGTGTGAC TGAGAGGAAA TTCAGCCAGA ACCTGGTTGC CCAGCTTTGG 
TACTTTGTGA AATGTGTTTA CTTCGGGTTG TCTGCTTACC AGATCCGTTG TGGCTACCCA 
ACGCGAGTCC TGGGGAACTT CCTCACCAAG AGCTACAATT ACGTCAACCT CTTCTTATTC 
CAAGGGTTTC GCCTCGTGCC CTTTTTGACT GAGCTGAGGG CAGTGATGGA CTGGGTGTGG 
ACGGACACAA CTTTGAGCCT GTCCAGCTGG ATCTGTGTGG AGGACATCTA TGCTCACATA 
TTCATCCTGA AGTGTTGGCG GGAGTCGGAG AAGAGATACC CTCAGCCACG GGGCCAGAAG 
AAGAAGAAAG TGGTGAAGTA TGGCATGGGA GGAATGATCA TCGTCCTGCT CATCTGCATT 
GTCTGGTTTC CTCTTCTCTT CATGTCTTTG ATCAAATCTG TGGCTGGGGT CATCAACCAG 
CCCCTGGACG TCTCCGTCAC AATTACCCTG GGAGGGTATC AGCCTATTTT CACAATGAGT 
GCCCAACAAA GCCAGTTGAA AGTTATGGAC CAGCAGAGCT TTAACAAATT TATACAAGCT 
TTTTCTAGGG ACACCGGTGC TATGCAATTT CTGGAAAATT ATGAAAAAGA AGACATAACA 
GTAGCAGAAC TGGAAGGAAA CTCAAATTCT TTGTGGACCA TCAGCCCACC CAGTAAGCAG 
AAAATGATAC ACGAACTCCT GGACCCCAAT AGTAGCTTCT CTGTTGTTTT TTCATGGAGT 
ATTCAGAGAA ACTTAAGTCT GGGTGCAAAA TCGGAAATAG CAACAGATAA GCTTTCTTTT 
CCTCTTAAAA ATATTACTCG AAAGAATATC GCTAAAATGA TAGCAGGCAA CAGCACAGAA 
AGTTCAAAAA CACCAGTGAC CATAGAAAAG ATTTATCCAT ATTATGTGAA AGCACCTAGT 
GATTCTAACT CAAAACCTAT AAAGCAACTT TTATCTGAAA ATAATTTCAT GGATATTACC 
ATCATTTTGT CCAGAGACAA TACAACTAAA TATAACAGTG AGTGGTGGGT TCTCAACCTG 
ACTGGAAACA GAATATACAA TCCGAACTCT CAGGCCCTGG AACTGGTGGT CTTCAATGAC 
AAAGTCAGTC CCCCAAGTCT GGGGTTCCTG GCTGGCTATG GTATTATGGG ATTATATGCT 
TCAGTTGTCC TTGTGATTGG GAAATTTGTC CGTGAATTCT TCAGTGGGAT TTCTCACTCC 
ATCATGTTTG AAGAGCTTCC AAATGTGGAT CGAATTTTGA AGTTGTGCAC AGATATTTTT 
TTAGTTCGAG AGACAGGAGA ACTGGAGCTA GAAGAAGATC TCTATGCCAA ATTAATATTC 
CTATATCGCT CACCAGAGAC AATGATCAAA TGGACTAGAG AAAAAACAAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIEZO2 ( XM_011525724.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524026.1 Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X3, mRNA.

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CloneID	OHu71132
Clone ID Related Accession (Same CDS sequence)	XM_011525724.3 , XM_011525724.2
Accession Version	XM_011525724.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	piezo-type mechanosensitive ion channel component 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525724.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG AAGTGGTGTG CGGGCTCATC TTCAGGCTGC TGCTGCCCAT CTGCCTGGCA 
GTAGCATGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC 
ATTCCTCTGT TCTCAGAACC AACAAAAACG ACGATGCAAG GACATACGGG ACGGTTATTA 
AAGTCTCTGT GCTTCATCAG TCTTTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG 
TTGGTGAGCC TTGAAGCTCA ACATCGTATT GCACCTGGCT ACAACTGCTC AACATGGGAA 
AAGACATTCC GGCAGATCGG CTTTGAAAGC TTAAAGGGAG CTGATGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCCTCTGT 
AGAAACATTG TTCAGAAACC TGTGACAGAC GAAGCAGCAC AGAGTAACCC GGAGTTTGAA 
AATGAAGAAT TGGCTGAAGG AGAAAAAATT GATTCAGAAG AGGCACTGAT CTATGAAGAG 
GATTTCAATG GAGGAGATGG TGTTGAAGGC GAGTTGGAAG AAAGCACGAA GTTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCCTCTAAG CTCAAGGAGT TCATTGGCAA CATGATCACC 
ACTGCTGGGA AAGTCGTTGT TACCATCTTA CTGGGCTCCT CGGGCATGAT GTTGCCGTCT 
TTGACATCAT CTGTGTATTT TTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACTGCT 
GGACATTTGA TTGGACTTTA TTTATACCAG TTCCAATTCT TTCAAGAGGC AGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAGTCAGTAA TTCAAACGGA CTGTTCAAGT 
ACTTGGAAGA TCATAGTGAA CCCGGACCTG TCGTGGTACC ACCACGCCAA CCCTATCCTC 
CTGCTGGTGA TGTACTACAC TCTGGCCACT CTGATCCGCA TCTGGCTGCA AGAGCCCCTT 
GTGCAGGATG AGGGGACCAA AGAAGAGGAC AAAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACAGCGGGGA GGAGGCGGAG CCTGTGGTAC GCAACCCATT ACCCCACTGA TGAGAGAAAA 
CTTTTATCCA TGACCCAGGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC 
GGCAACCCCG TGGATTACCA CACCATCCAC CCAAGCCTGC CCATGGAGAA CGGCCCTGGC 
AAAGCCGACC TCTACTCCAC CCCTCAGTAC CGGTGGGAGC CCTCTGATGA ATCCTCAGAA 
AAGCGAGAGG AGGAAGAGGA AGAGAAAGAA GAATTTGAAG AAGAAAGGAG CCGTGAGGAA 
AAAAGAAGTA TCAAAGTTCA TGCCATGGTC TCCGTATTCC AATTTATTAT GAAACAAAGT 
TACATCTGTG CCCTCATAGC TATGATGGCC TGGAGCATCA CCTATCACAG CTGGCTGACC 
TTCGTGCTGC TGATCTGGTC GTGCACTCTT TGGATGATTC GCAACAGAAG AAAATATGCC 
ATGATCAGCT CTCCCTTCAT GGTGGTTTAT GGAAACCTAT TGTTGATATT ACAGTATATA 
TGGAGTTTTG AACTTCCTGA AATTAAAAAA GTTCCAGGAT TTTTAGAAAA GAAAGAGCCA 
GGAGAACTTG CTTCCAAAAT CCTTTTCACC ATTACTTTTT GGCTACTGCT GAGGCAGCAC 
CTCACAGAGC AAAAAGCTCT GCAAGAAAAG GAAGCTCTTT TATCGGAAGT CAAAATTGGC 
AGTCAGGAAA ATGAAGAAAA AGATGAGGAA CTTCAAGATA TACAAGTGGA AGGAGAGCCC 
AAAGAGGAGG AAGAAGAGGA AGCGAAGGAA GAGAAGCAAG AGAGAAAGAA GGTAGAGCAA 
GAGGAAGCTG AAGAAGAAGA TGAGCAGGAC ATCATGAAAG TCCTGGGCAA TCTGGTGGTG 
GCCATGTTCA TCAAGTACTG GATCTACGTC TGCGGAGGCA TGTTCTTCTT CGTCAGCTTC 
GAGGGTAAAA TCGTAATGTA CAAAATCATC TACATGGTGC TGTTCCTGTT CTGTGTGGCC 
CTATACCAGG TGCACTATGA ATGGTGGAGG AAAATTCTAA AATATTTTTG GATGTCAGTG 
GTTATTTACA CTATGCTGGT GCTTATCTTT ATATACACAT ATCAGTTTGA GAACTTCCCA 
GGCCTGTGGC AAAATATGAC TGGACTGAAA AAAGAAAAGC TTGAGGATCT TGGCTTAAAG 
CAGTTTACTG TGGCTGAACT ATTCACTCGC ATATTCATCC CAACCTCCTT TCTGCTGGTG 
TGCATTTTAC ACCTGCACTA CTTCCATGAC CGGTTCCTTG AACTCACAGA CCTCAAGTCC 
ATTCCCAGCA AAGAAGACAA CACCATCTAC AGCCATGCCA AAGTCAATGG TCGCGTATAT 
CTAATAATAA ATAGCATCAA GAAAAAATTA CCAATCCACC AAAATGAACT GGCCCACCCG 
GAAGGAAGCC TCCCGGACCT CACCATGATG CATCTGACTG CCAGCCTGGA GAAGCCGGAG 
GTGAGGAAGT TGGCTGAGCC TGGGGAGGAG AAGCTTGAGG GCTACTCTGA AAAAGCCCAG 
AAGGGTGATC TTGGGAAAGA CAGCGAGGAG TCAGAGGAGG ACGGAGAGGA AGAGGAGGAA 
TCCGAGGAGG AGGAAGAAAC ATCAGACTTA AGGAACAAAT GGCACCTGGT GATTGACCGC 
CTCACTGTGC TCTTCTTAAA ATTCCTGGAG TATTTTCACA AGCTGCAGGT GTTCATGTGG 
TGGATTTTGG AGTTGCACAT CATCAAAATC GTTTCCTCTT ACATTATCTG GGTTTCTGTG 
AAAGAGGTGT CTCTGTTCAA CTATGTATTT TTGATTTCTT GGGCTTTTGC TCTGCCGTAC 
GCCAAGCTGC GCCGTCTGGC TTCAAGTGTC TGCACAGTCT GGACGTGTGT GATCATCGTC 
TGCAAAATGT TGTACCAGCT CCAAACCATT AAGCCTGAGA ACTTCTCTGT TAACTGTTCC 
TTGCCAAATG AAAATCAAAC AAACATCCCC TTTAATGAGT TGAACAAGTC TCTGCTCTAC 
AGCGCTCCTA TCGATCCTAC AGAGTGGGTC GGCCTGCGGA AGTCTTCGCC TCTGCTAGTC 
TACCTGAGGA ATAACCTCCT GATGCTGGCT ATCCTGGCCT TTGAAGTCAC CATTTACCGC 
CATCAGGAAT ACTATCGAGG TCGAAATAAC CTGACGGCCC CTGTGTCTAG AACTATCTTT 
CATGACATTA CAAGACTACA TCTAGATGAT GGACTTATTA ATTGTGCCAA ATATTTCATT 
AATTACTTCT TTTACAAGTT TGGTCTGGAG ACCTGTTTCC TAATGTCAGT TAACGTCATT 
GGCCAGCGAA TGGATTTCTA TGCCATGATC CACGCCTGCT GGCTGATCGC TGTCTTATAT 
AGACGCAGAA GGAAAGCCAT CGCAGAGATC TGGCCCAAGT ACTGCTGCTT CCTGGCATGC 
ATCATCACCT TCCAGTATTT CATCTGCATT GGCATCCCAC CTGCTCCTTG CCGAGATTAC 
CCGTGGAGAT TCAAGGGTGC CAGCTTCAAT GACAACATCA TAAAGTGGCT GTACTTCCCA 
GATTTCATTG TGCGGCCCAA CCCTGTGTTT CTCGTCTATG ACTTCATGCT GCTTCTGTGT 
GCCTCCTTAC AACGGCAGAT TTTTGAGGAT GAGAACAAGG CTGCAGTGCG AATCATGGCA 
GGTGACAATG TCGAGATCTG CATGAACCTT GATGCGGCCT CCTTCAGCCA ACATAACCCT 
GTGCCAGATT TTATTCACTG CAGATCTTAC TTAGACATGT CCAAAGTGAT CATCTTCAGC 
TACCTCTTCT GGTTTGTGCT CACCATCATC TTCATCACTG GGACCACCAG GATCAGCATC 
TTTTGCATGG GGTACCTGGT GGCCTGTTTC TACTTCCTGC TCTTTGGGGG CGATTTGCTG 
TTGAAACCCA TCAAGAGCAT CCTGCGCTAC TGGGACTGGC TGATCGCATA CAACGTTTTT 
GTGATTACGA TGAAAAATAT CCTGTCAATA GGAGCATGTG GATACATTGG AACATTGGTG 
CACAATAGTT GTTGGTTGAT CCAGGCTTTC AGCCTGGCCT GCACAGTCAA AGGCTATCAA 
ATGCCTGCTG CTAATTCACC CTGTACACTT CCCAGTGGGG AAGCAGGAAT CATTTGGGAC 
AGCATATGTT TTGCCTTCCT CCTGCTGCAA AGAAGAGTTT TCATGAGTTA TTATTTTCTA 
CATGTTGTGG CTGATATAAA AGCTTCCCAG ATTCTGGCAT CAAGAGGAGC TGAACTTTTC 
CAGGCCACAA TTGTAAAAGC TGTAAAGGCA AGAATTGAGG AAGAGAAGAA GTCCATGGAC 
CAGCTGAAGC GACAGATGGA TCGCATCAAG GCCAGGCAAC AGAAATATAA AAAGGGTAAG 
GAGAGGATGC TGAGCTTGAC CCAGGAGCCA GGGGAAGGCC AGGACATGCA AAAACTCTCT 
GAAGAGGATG ATGAAAGAGA AGCAGACAAA CAGAAAGCCA AGGGCAAAAA AAAGCAGTGG 
TGGCGGCCTT GGGTTGATCA TGCTTCCATG GTCAGGAGTG GAGATTATTA TTTGTTTGAA 
ACGGATAGTG AAGAGGAGGA AGAGGAAGAA TTAAAGAAGG AAGATGAAGA ACCTCCACGA 
AGGTCAGCAT TCCAGTTTGT TTATCAAGCC TGGATTACTG ATCCTAAAAC AGCACTCCGA 
CAAAGACACA AAGAGAAAAA AAGGTCTGCA AGAGAAGAAC GGAAACGAAG GCGGAAAGGA 
TCCAAGGAGG GTCCTGTGGA ATGGGAAGAC CGGGAGGATG AACCAATCAA AAAGAAATCC 
GATGGACCAG ATAATATCAT CAAGAGGATA TTTAATATTT TGAAATTTAC CTGGGTCCTA 
TTTCTGGCAA CAGTGGACAG TTTCACTACT TGGCTTAACT CCATTTCAAG GGAGCATATT 
GATATATCTA CAGTTCTGAG AATTGAACGA TGCATGCTGA CCAGAGAAAT TAAGAAGGGC 
AATGTTCCAA CTCGGGAGAG CATCCACATG TACTATCAGA ACCACATCAT GAACCTTTCC 
AGAGAGTCGG GACTGGACAC CATTGACGAG CATCCCGGAG CTGCTTCAGG TGCACAGACA 
GCCCACAGGA TGGATAGTTT AGATTCACAT GACAGTATCT CCAGCGAGCC CACGCAGTGT 
ACCATGCTGT ACTCACGCCA GGGGACCACT GAGACCATCG AGGAGGTGGA GGCTGAGCAG 
GAGGAGGAGG CAGGGAGCAC GGCGCCTGAG CCCAGGGAGG CCAAGGAGTA CGAGGCCACT 
GGGTACGATG TGGGAGCCAT GGGTGCCGAG GAGGCCAGCC TCACCCCAGA GGAAGAGCTG 
ACACAGTTCT CCACCTTGGA CGGGGATGTG GAGGCCCCAC CCTCCTACAG CAAGGCTGTG 
AGCTTCGAGC ATCTGTCCTT CGGCTCGCAG GACGACTCTG CAGGCAAGAA CCGTATGGCA 
GTCAGCCCGG ACGACAGCCG CACCGACAAG CTGGGGTCCA GCATCTTACC TCCCCTGACC 
CATGAGCTGA CGGCCAGCGA GCTGCTGCTG AAAAAGATGT TTCACGACGA TGAGCTTGAA 
GAGTCAGAGA AATTCTACGT GGGGCAGCCC CGATTTCTGC TGCTCTTCTA TGCCATGTAC 
AATACCCTGG TGGCCCGCTC GGAGATGGTG TGCTACTTCG TGATCATCCT CAACCACATG 
GTCTCTGCCT CCATGATCAC GCTCCTGCTT CCCATCCTCA TCTTCCTCTG GGCCATGTTG 
TCCGTCCCCA GGCCCAGCCG CCGGTTCTGG ATGATGGCCA TCGTCTATAC TGAGGTGGCA 
ATTGTAGTCA AGTATTTCTT CCAATTTGGG TTCTTTCCCT GGAATAAGAA TGTGGAGGTG 
AACAAAGATA AACCGTATCA CCCCCCAAAC ATCATAGGAG TGGAAAAGAA GGAAGGTTAT 
GTTCTCTATG ACCTCATCCA GCTCCTGGCT CTGTTCTTTC ATCGATCAAT TTTGAAGTGC 
CATGGCTTAT GGGATGAAGA TGACATGACT GAAAGTGGCA TGGCCAGGGA GGAATCAGAT 
GATGAGCTCT CCCTCGGTCA TGGCAGGAGG GACTCCTCCG ATTCTCTCAA GTCCATCAAC 
CTGGCCGCGT CTGTGGAGTC AGTGCATGTG ACCTTCCCGG AGCAGCAGAC AGCTGTCCGG 
AGGAAGCGCT CCGGCAGCAG CTCCGAGCCA TCCCAGAGAT CCAGCTTTTC TTCAAACAGA 
TCCCAAAGAG GCAGCACAAG CACCCGAAAC AGCAGTCAAA AAGGAAGCAG TGTTTTGAGT 
ATTAAGCAAA AAGGCAAAAG GGAACTTTAT ATGGAAAAGC TTCAAGAACA TTTAATCAAA 
GCAAAAGCCT TTACCATAAA GAAGACGCTG GAGATCTATG TGCCCATCAA ACAGTTCTTT 
TACAACCTCA TCCACCCGGA GTATAGCGCC GTGACTGACG TGTATGTACT CATGTTCCTG 
GCTGACACTG TGGACTTCAT CATCATTGTC TTCGGCTTTT GGGCCTTTGG GAAACACTCA 
GCAGCTGCAG ACATCACCTC TTCACTGTCA GAGGACCAGG TCCCGGGGCC GTTTTTGGTG 
ATGGTCCTCA TTCAGTTTGG AACCATGGTG GTGGACCGAG CCCTCTACCT CAGGAAGACT 
GTACTGGGAA AGGTCATCTT CCAGGTCATT CTTGTGTTCG GAATTCACTT CTGGATGTTC 
TTCATCTTAC CTGGTGTGAC TGAGAGGAAA TTCAGCCAGA ACCTGGTTGC CCAGCTTTGG 
TACTTTGTGA AATGTGTTTA CTTCGGGTTG TCTGCTTACC AGATCCGTTG TGGCTACCCA 
ACGCGAGTCC TGGGGAACTT CCTCACCAAG AGCTACAATT ACGTCAACCT CTTCTTATTC 
CAAGGGTTTC GCCTCGTGCC CTTTTTGACT GAGCTGAGGG CAGTGATGGA CTGGGTGTGG 
ACGGACACAA CTTTGAGCCT GTCCAGCTGG ATCTGTGTGG AGGACATCTA TGCTCACATA 
TTCATCCTGA AGTGTTGGCG GGAGTCGGAG AAGAGATACC CTCAGCCACG GGGCCAGAAG 
AAGAAGAAAG TGGTGAAGTA TGGCATGGGA GGAATGATCA TCGTCCTGCT CATCTGCATT 
GTCTGGTTTC CTCTTCTCTT CATGTCTTTG ATCAAATCTG TGGCTGGGGT CATCAACCAG 
CCCCTGGACG TCTCCGTCAC AATTACCCTG GGAGGGTATC AGCCTATTTT CACAATGAGT 
GCCCAACAAA GCCAGTTGAA AGTTATGGAC CAGCAGAGCT TTAACAAATT TATACAAGCT 
TTTTCTAGGG ACACCGGTGC TATGCAATTT CTGGAAAATT ATGAAAAAGA AGACATAACA 
GTAGCAGAAC TGGAAGGAAA CTCAAATTCT TTGTGGACCA TCAGCCCACC CAGTAAGCAG 
AAAATGATAC ACGAACTCCT GGACCCCAAT AGTAGCTTCT CTGTTGTTTT TTCATGGAGT 
ATTCAGAGAA ACTTAAGTCT GGGTGCAAAA TCGGAAATAG CAACAGATAA GCTTTCTTTT 
CCTCTTAAAA ATATTACTCG AAAGAATATC GCTAAAATGA TAGCAGGCAA CAGCACAGAA 
AGTTCAAAAA CACCAGTGAC CATAGAAAAG ATTTATCCAT ATTATGTGAA AGCACCTAGT 
GATTCTAACT CAAAACCTAT AAAGCAACTT TTATCTGAAA ATAATTTCAT GGATATTACC 
ATCATTTTGT CCAGAGACAA TACAACTAAA TATAACAGTG AGTGGTGGGT TCTCAACCTG 
ACTGGAAACA GAATATACAA TCCGAACTCT CAGGCCCTGG AACTGGTGGT CTTCAATGAC 
AAAGTCAGTC CCCCAAGTCT GGGGTTCCTG GCTGGCTATG GTATTATGGG ATTATATGCT 
TCAGTTGTCC TTGTGATTGG GAAATTTGTC CGTGAATTCT TCAGTGGGAT TTCTCACTCC 
ATCATGTTTG AAGAGCTTCC AAATGTGGAT CGAATTTTGA AGTTGTGCAC AGATATTTTT 
TTAGTTCGAG AGACAGGAGA ACTGGAGCTA GAAGAAGATC TCTATGCCAA ATTAATATTC 
CTATATCGCT CACCAGAGAC AATGATCAAA TGGACTAGAG AAAAAACAAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524026.1
CDS	973..9306
Translation	MASEVVCGLIFRLLLPICLAVACAFRYNGLSFVYLIYLLLIPLFSEPTKTTMQGHTGRLLKSLCFISLSFLLLHIIFHITLVSLEAQHRIAPGYNCSTWEKTFRQIGFESLKGADAGNGIRVFVPDIGMFIASLTIWLLCRNIVQKPVTDEAAQSNPEFENEELAEGEKIDSEEALIYEEDFNGGDGVEGELEESTKLKMFRRLASVASKLKEFIGNMITTAGKVVVTILLGSSGMMLPSLTSSVYFFVFLGLCTWWSWCRTFDPLLFSCLCVLLAIFTAGHLIGLYLYQFQFFQEAVPPNDYYARLFGIKSVIQTDCSSTWKIIVNPDLSWYHHANPILLLVMYYTLATLIRIWLQEPLVQDEGTKEEDKALACSPIQITAGRRRSLWYATHYPTDERKLLSMTQDDYKPSDGLLVTVNGNPVDYHTIHPSLPMENGPGKADLYSTPQYRWEPSDESSEKREEEEEEKEEFEEERSREEKRSIKVHAMVSVFQFIMKQSYICALIAMMAWSITYHSWLTFVLLIWSCTLWMIRNRRKYAMISSPFMVVYGNLLLILQYIWSFELPEIKKVPGFLEKKEPGELASKILFTITFWLLLRQHLTEQKALQEKEALLSEVKIGSQENEEKDEELQDIQVEGEPKEEEEEEAKEEKQERKKVEQEEAEEEDEQDIMKVLGNLVVAMFIKYWIYVCGGMFFFVSFEGKIVMYKIIYMVLFLFCVALYQVHYEWWRKILKYFWMSVVIYTMLVLIFIYTYQFENFPGLWQNMTGLKKEKLEDLGLKQFTVAELFTRIFIPTSFLLVCILHLHYFHDRFLELTDLKSIPSKEDNTIYSHAKVNGRVYLIINSIKKKLPIHQNELAHPEGSLPDLTMMHLTASLEKPEVRKLAEPGEEKLEGYSEKAQKGDLGKDSEESEEDGEEEEESEEEEETSDLRNKWHLVIDRLTVLFLKFLEYFHKLQVFMWWILELHIIKIVSSYIIWVSVKEVSLFNYVFLISWAFALPYAKLRRLASSVCTVWTCVIIVCKMLYQLQTIKPENFSVNCSLPNENQTNIPFNELNKSLLYSAPIDPTEWVGLRKSSPLLVYLRNNLLMLAILAFEVTIYRHQEYYRGRNNLTAPVSRTIFHDITRLHLDDGLINCAKYFINYFFYKFGLETCFLMSVNVIGQRMDFYAMIHACWLIAVLYRRRRKAIAEIWPKYCCFLACIITFQYFICIGIPPAPCRDYPWRFKGASFNDNIIKWLYFPDFIVRPNPVFLVYDFMLLLCASLQRQIFEDENKAAVRIMAGDNVEICMNLDAASFSQHNPVPDFIHCRSYLDMSKVIIFSYLFWFVLTIIFITGTTRISIFCMGYLVACFYFLLFGGDLLLKPIKSILRYWDWLIAYNVFVITMKNILSIGACGYIGTLVHNSCWLIQAFSLACTVKGYQMPAANSPCTLPSGEAGIIWDSICFAFLLLQRRVFMSYYFLHVVADIKASQILASRGAELFQATIVKAVKARIEEEKKSMDQLKRQMDRIKARQQKYKKGKERMLSLTQEPGEGQDMQKLSEEDDEREADKQKAKGKKKQWWRPWVDHASMVRSGDYYLFETDSEEEEEEELKKEDEEPPRRSAFQFVYQAWITDPKTALRQRHKEKKRSAREERKRRRKGSKEGPVEWEDREDEPIKKKSDGPDNIIKRIFNILKFTWVLFLATVDSFTTWLNSISREHIDISTVLRIERCMLTREIKKGNVPTRESIHMYYQNHIMNLSRESGLDTIDEHPGAASGAQTAHRMDSLDSHDSISSEPTQCTMLYSRQGTTETIEEVEAEQEEEAGSTAPEPREAKEYEATGYDVGAMGAEEASLTPEEELTQFSTLDGDVEAPPSYSKAVSFEHLSFGSQDDSAGKNRMAVSPDDSRTDKLGSSILPPLTHELTASELLLKKMFHDDELEESEKFYVGQPRFLLLFYAMYNTLVARSEMVCYFVIILNHMVSASMITLLLPILIFLWAMLSVPRPSRRFWMMAIVYTEVAIVVKYFFQFGFFPWNKNVEVNKDKPYHPPNIIGVEKKEGYVLYDLIQLLALFFHRSILKCHGLWDEDDMTESGMAREESDDELSLGHGRRDSSDSLKSINLAASVESVHVTFPEQQTAVRRKRSGSSSEPSQRSSFSSNRSQRGSTSTRNSSQKGSSVLSIKQKGKRELYMEKLQEHLIKAKAFTIKKTLEIYVPIKQFFYNLIHPEYSAVTDVYVLMFLADTVDFIIIVFGFWAFGKHSAAADITSSLSEDQVPGPFLVMVLIQFGTMVVDRALYLRKTVLGKVIFQVILVFGIHFWMFFILPGVTERKFSQNLVAQLWYFVKCVYFGLSAYQIRCGYPTRVLGNFLTKSYNYVNLFLFQGFRLVPFLTELRAVMDWVWTDTTLSLSSWICVEDIYAHIFILKCWRESEKRYPQPRGQKKKKVVKYGMGGMIIVLLICIVWFPLLFMSLIKSVAGVINQPLDVSVTITLGGYQPIFTMSAQQSQLKVMDQQSFNKFIQAFSRDTGAMQFLENYEKEDITVAELEGNSNSLWTISPPSKQKMIHELLDPNSSFSVVFSWSIQRNLSLGAKSEIATDKLSFPLKNITRKNIAKMIAGNSTESSKTPVTIEKIYPYYVKAPSDSNSKPIKQLLSENNFMDITIILSRDNTTKYNSEWWVLNLTGNRIYNPNSQALELVVFNDKVSPPSLGFLAGYGIMGLYASVVLVIGKFVREFFSGISHSIMFEELPNVDRILKLCTDIFLVRETGELELEEDLYAKLIFLYRSPETMIKWTREKTN

Target ORF information:

RefSeq Version	XM_011525724.2
Organism	Homo sapiens(human)
Definition	Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525724.2

ORF Insert Sequence:

ATGGCCTCAG AAGTGGTGTG CGGGCTCATC TTCAGGCTGC TGCTGCCCAT CTGCCTGGCA 
GTAGCATGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC 
ATTCCTCTGT TCTCAGAACC AACAAAAACG ACGATGCAAG GACATACGGG ACGGTTATTA 
AAGTCTCTGT GCTTCATCAG TCTTTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG 
TTGGTGAGCC TTGAAGCTCA ACATCGTATT GCACCTGGCT ACAACTGCTC AACATGGGAA 
AAGACATTCC GGCAGATCGG CTTTGAAAGC TTAAAGGGAG CTGATGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCCTCTGT 
AGAAACATTG TTCAGAAACC TGTGACAGAC GAAGCAGCAC AGAGTAACCC GGAGTTTGAA 
AATGAAGAAT TGGCTGAAGG AGAAAAAATT GATTCAGAAG AGGCACTGAT CTATGAAGAG 
GATTTCAATG GAGGAGATGG TGTTGAAGGC GAGTTGGAAG AAAGCACGAA GTTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCCTCTAAG CTCAAGGAGT TCATTGGCAA CATGATCACC 
ACTGCTGGGA AAGTCGTTGT TACCATCTTA CTGGGCTCCT CGGGCATGAT GTTGCCGTCT 
TTGACATCAT CTGTGTATTT TTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACTGCT 
GGACATTTGA TTGGACTTTA TTTATACCAG TTCCAATTCT TTCAAGAGGC AGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAGTCAGTAA TTCAAACGGA CTGTTCAAGT 
ACTTGGAAGA TCATAGTGAA CCCGGACCTG TCGTGGTACC ACCACGCCAA CCCTATCCTC 
CTGCTGGTGA TGTACTACAC TCTGGCCACT CTGATCCGCA TCTGGCTGCA AGAGCCCCTT 
GTGCAGGATG AGGGGACCAA AGAAGAGGAC AAAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACAGCGGGGA GGAGGCGGAG CCTGTGGTAC GCAACCCATT ACCCCACTGA TGAGAGAAAA 
CTTTTATCCA TGACCCAGGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC 
GGCAACCCCG TGGATTACCA CACCATCCAC CCAAGCCTGC CCATGGAGAA CGGCCCTGGC 
AAAGCCGACC TCTACTCCAC CCCTCAGTAC CGGTGGGAGC CCTCTGATGA ATCCTCAGAA 
AAGCGAGAGG AGGAAGAGGA AGAGAAAGAA GAATTTGAAG AAGAAAGGAG CCGTGAGGAA 
AAAAGAAGTA TCAAAGTTCA TGCCATGGTC TCCGTATTCC AATTTATTAT GAAACAAAGT 
TACATCTGTG CCCTCATAGC TATGATGGCC TGGAGCATCA CCTATCACAG CTGGCTGACC 
TTCGTGCTGC TGATCTGGTC GTGCACTCTT TGGATGATTC GCAACAGAAG AAAATATGCC 
ATGATCAGCT CTCCCTTCAT GGTGGTTTAT GGAAACCTAT TGTTGATATT ACAGTATATA 
TGGAGTTTTG AACTTCCTGA AATTAAAAAA GTTCCAGGAT TTTTAGAAAA GAAAGAGCCA 
GGAGAACTTG CTTCCAAAAT CCTTTTCACC ATTACTTTTT GGCTACTGCT GAGGCAGCAC 
CTCACAGAGC AAAAAGCTCT GCAAGAAAAG GAAGCTCTTT TATCGGAAGT CAAAATTGGC 
AGTCAGGAAA ATGAAGAAAA AGATGAGGAA CTTCAAGATA TACAAGTGGA AGGAGAGCCC 
AAAGAGGAGG AAGAAGAGGA AGCGAAGGAA GAGAAGCAAG AGAGAAAGAA GGTAGAGCAA 
GAGGAAGCTG AAGAAGAAGA TGAGCAGGAC ATCATGAAAG TCCTGGGCAA TCTGGTGGTG 
GCCATGTTCA TCAAGTACTG GATCTACGTC TGCGGAGGCA TGTTCTTCTT CGTCAGCTTC 
GAGGGTAAAA TCGTAATGTA CAAAATCATC TACATGGTGC TGTTCCTGTT CTGTGTGGCC 
CTATACCAGG TGCACTATGA ATGGTGGAGG AAAATTCTAA AATATTTTTG GATGTCAGTG 
GTTATTTACA CTATGCTGGT GCTTATCTTT ATATACACAT ATCAGTTTGA GAACTTCCCA 
GGCCTGTGGC AAAATATGAC TGGACTGAAA AAAGAAAAGC TTGAGGATCT TGGCTTAAAG 
CAGTTTACTG TGGCTGAACT ATTCACTCGC ATATTCATCC CAACCTCCTT TCTGCTGGTG 
TGCATTTTAC ACCTGCACTA CTTCCATGAC CGGTTCCTTG AACTCACAGA CCTCAAGTCC 
ATTCCCAGCA AAGAAGACAA CACCATCTAC AGCCATGCCA AAGTCAATGG TCGCGTATAT 
CTAATAATAA ATAGCATCAA GAAAAAATTA CCAATCCACC AAAATGAACT GGCCCACCCG 
GAAGGAAGCC TCCCGGACCT CACCATGATG CATCTGACTG CCAGCCTGGA GAAGCCGGAG 
GTGAGGAAGT TGGCTGAGCC TGGGGAGGAG AAGCTTGAGG GCTACTCTGA AAAAGCCCAG 
AAGGGTGATC TTGGGAAAGA CAGCGAGGAG TCAGAGGAGG ACGGAGAGGA AGAGGAGGAA 
TCCGAGGAGG AGGAAGAAAC ATCAGACTTA AGGAACAAAT GGCACCTGGT GATTGACCGC 
CTCACTGTGC TCTTCTTAAA ATTCCTGGAG TATTTTCACA AGCTGCAGGT GTTCATGTGG 
TGGATTTTGG AGTTGCACAT CATCAAAATC GTTTCCTCTT ACATTATCTG GGTTTCTGTG 
AAAGAGGTGT CTCTGTTCAA CTATGTATTT TTGATTTCTT GGGCTTTTGC TCTGCCGTAC 
GCCAAGCTGC GCCGTCTGGC TTCAAGTGTC TGCACAGTCT GGACGTGTGT GATCATCGTC 
TGCAAAATGT TGTACCAGCT CCAAACCATT AAGCCTGAGA ACTTCTCTGT TAACTGTTCC 
TTGCCAAATG AAAATCAAAC AAACATCCCC TTTAATGAGT TGAACAAGTC TCTGCTCTAC 
AGCGCTCCTA TCGATCCTAC AGAGTGGGTC GGCCTGCGGA AGTCTTCGCC TCTGCTAGTC 
TACCTGAGGA ATAACCTCCT GATGCTGGCT ATCCTGGCCT TTGAAGTCAC CATTTACCGC 
CATCAGGAAT ACTATCGAGG TCGAAATAAC CTGACGGCCC CTGTGTCTAG AACTATCTTT 
CATGACATTA CAAGACTACA TCTAGATGAT GGACTTATTA ATTGTGCCAA ATATTTCATT 
AATTACTTCT TTTACAAGTT TGGTCTGGAG ACCTGTTTCC TAATGTCAGT TAACGTCATT 
GGCCAGCGAA TGGATTTCTA TGCCATGATC CACGCCTGCT GGCTGATCGC TGTCTTATAT 
AGACGCAGAA GGAAAGCCAT CGCAGAGATC TGGCCCAAGT ACTGCTGCTT CCTGGCATGC 
ATCATCACCT TCCAGTATTT CATCTGCATT GGCATCCCAC CTGCTCCTTG CCGAGATTAC 
CCGTGGAGAT TCAAGGGTGC CAGCTTCAAT GACAACATCA TAAAGTGGCT GTACTTCCCA 
GATTTCATTG TGCGGCCCAA CCCTGTGTTT CTCGTCTATG ACTTCATGCT GCTTCTGTGT 
GCCTCCTTAC AACGGCAGAT TTTTGAGGAT GAGAACAAGG CTGCAGTGCG AATCATGGCA 
GGTGACAATG TCGAGATCTG CATGAACCTT GATGCGGCCT CCTTCAGCCA ACATAACCCT 
GTGCCAGATT TTATTCACTG CAGATCTTAC TTAGACATGT CCAAAGTGAT CATCTTCAGC 
TACCTCTTCT GGTTTGTGCT CACCATCATC TTCATCACTG GGACCACCAG GATCAGCATC 
TTTTGCATGG GGTACCTGGT GGCCTGTTTC TACTTCCTGC TCTTTGGGGG CGATTTGCTG 
TTGAAACCCA TCAAGAGCAT CCTGCGCTAC TGGGACTGGC TGATCGCATA CAACGTTTTT 
GTGATTACGA TGAAAAATAT CCTGTCAATA GGAGCATGTG GATACATTGG AACATTGGTG 
CACAATAGTT GTTGGTTGAT CCAGGCTTTC AGCCTGGCCT GCACAGTCAA AGGCTATCAA 
ATGCCTGCTG CTAATTCACC CTGTACACTT CCCAGTGGGG AAGCAGGAAT CATTTGGGAC 
AGCATATGTT TTGCCTTCCT CCTGCTGCAA AGAAGAGTTT TCATGAGTTA TTATTTTCTA 
CATGTTGTGG CTGATATAAA AGCTTCCCAG ATTCTGGCAT CAAGAGGAGC TGAACTTTTC 
CAGGCCACAA TTGTAAAAGC TGTAAAGGCA AGAATTGAGG AAGAGAAGAA GTCCATGGAC 
CAGCTGAAGC GACAGATGGA TCGCATCAAG GCCAGGCAAC AGAAATATAA AAAGGGTAAG 
GAGAGGATGC TGAGCTTGAC CCAGGAGCCA GGGGAAGGCC AGGACATGCA AAAACTCTCT 
GAAGAGGATG ATGAAAGAGA AGCAGACAAA CAGAAAGCCA AGGGCAAAAA AAAGCAGTGG 
TGGCGGCCTT GGGTTGATCA TGCTTCCATG GTCAGGAGTG GAGATTATTA TTTGTTTGAA 
ACGGATAGTG AAGAGGAGGA AGAGGAAGAA TTAAAGAAGG AAGATGAAGA ACCTCCACGA 
AGGTCAGCAT TCCAGTTTGT TTATCAAGCC TGGATTACTG ATCCTAAAAC AGCACTCCGA 
CAAAGACACA AAGAGAAAAA AAGGTCTGCA AGAGAAGAAC GGAAACGAAG GCGGAAAGGA 
TCCAAGGAGG GTCCTGTGGA ATGGGAAGAC CGGGAGGATG AACCAATCAA AAAGAAATCC 
GATGGACCAG ATAATATCAT CAAGAGGATA TTTAATATTT TGAAATTTAC CTGGGTCCTA 
TTTCTGGCAA CAGTGGACAG TTTCACTACT TGGCTTAACT CCATTTCAAG GGAGCATATT 
GATATATCTA CAGTTCTGAG AATTGAACGA TGCATGCTGA CCAGAGAAAT TAAGAAGGGC 
AATGTTCCAA CTCGGGAGAG CATCCACATG TACTATCAGA ACCACATCAT GAACCTTTCC 
AGAGAGTCGG GACTGGACAC CATTGACGAG CATCCCGGAG CTGCTTCAGG TGCACAGACA 
GCCCACAGGA TGGATAGTTT AGATTCACAT GACAGTATCT CCAGCGAGCC CACGCAGTGT 
ACCATGCTGT ACTCACGCCA GGGGACCACT GAGACCATCG AGGAGGTGGA GGCTGAGCAG 
GAGGAGGAGG CAGGGAGCAC GGCGCCTGAG CCCAGGGAGG CCAAGGAGTA CGAGGCCACT 
GGGTACGATG TGGGAGCCAT GGGTGCCGAG GAGGCCAGCC TCACCCCAGA GGAAGAGCTG 
ACACAGTTCT CCACCTTGGA CGGGGATGTG GAGGCCCCAC CCTCCTACAG CAAGGCTGTG 
AGCTTCGAGC ATCTGTCCTT CGGCTCGCAG GACGACTCTG CAGGCAAGAA CCGTATGGCA 
GTCAGCCCGG ACGACAGCCG CACCGACAAG CTGGGGTCCA GCATCTTACC TCCCCTGACC 
CATGAGCTGA CGGCCAGCGA GCTGCTGCTG AAAAAGATGT TTCACGACGA TGAGCTTGAA 
GAGTCAGAGA AATTCTACGT GGGGCAGCCC CGATTTCTGC TGCTCTTCTA TGCCATGTAC 
AATACCCTGG TGGCCCGCTC GGAGATGGTG TGCTACTTCG TGATCATCCT CAACCACATG 
GTCTCTGCCT CCATGATCAC GCTCCTGCTT CCCATCCTCA TCTTCCTCTG GGCCATGTTG 
TCCGTCCCCA GGCCCAGCCG CCGGTTCTGG ATGATGGCCA TCGTCTATAC TGAGGTGGCA 
ATTGTAGTCA AGTATTTCTT CCAATTTGGG TTCTTTCCCT GGAATAAGAA TGTGGAGGTG 
AACAAAGATA AACCGTATCA CCCCCCAAAC ATCATAGGAG TGGAAAAGAA GGAAGGTTAT 
GTTCTCTATG ACCTCATCCA GCTCCTGGCT CTGTTCTTTC ATCGATCAAT TTTGAAGTGC 
CATGGCTTAT GGGATGAAGA TGACATGACT GAAAGTGGCA TGGCCAGGGA GGAATCAGAT 
GATGAGCTCT CCCTCGGTCA TGGCAGGAGG GACTCCTCCG ATTCTCTCAA GTCCATCAAC 
CTGGCCGCGT CTGTGGAGTC AGTGCATGTG ACCTTCCCGG AGCAGCAGAC AGCTGTCCGG 
AGGAAGCGCT CCGGCAGCAG CTCCGAGCCA TCCCAGAGAT CCAGCTTTTC TTCAAACAGA 
TCCCAAAGAG GCAGCACAAG CACCCGAAAC AGCAGTCAAA AAGGAAGCAG TGTTTTGAGT 
ATTAAGCAAA AAGGCAAAAG GGAACTTTAT ATGGAAAAGC TTCAAGAACA TTTAATCAAA 
GCAAAAGCCT TTACCATAAA GAAGACGCTG GAGATCTATG TGCCCATCAA ACAGTTCTTT 
TACAACCTCA TCCACCCGGA GTATAGCGCC GTGACTGACG TGTATGTACT CATGTTCCTG 
GCTGACACTG TGGACTTCAT CATCATTGTC TTCGGCTTTT GGGCCTTTGG GAAACACTCA 
GCAGCTGCAG ACATCACCTC TTCACTGTCA GAGGACCAGG TCCCGGGGCC GTTTTTGGTG 
ATGGTCCTCA TTCAGTTTGG AACCATGGTG GTGGACCGAG CCCTCTACCT CAGGAAGACT 
GTACTGGGAA AGGTCATCTT CCAGGTCATT CTTGTGTTCG GAATTCACTT CTGGATGTTC 
TTCATCTTAC CTGGTGTGAC TGAGAGGAAA TTCAGCCAGA ACCTGGTTGC CCAGCTTTGG 
TACTTTGTGA AATGTGTTTA CTTCGGGTTG TCTGCTTACC AGATCCGTTG TGGCTACCCA 
ACGCGAGTCC TGGGGAACTT CCTCACCAAG AGCTACAATT ACGTCAACCT CTTCTTATTC 
CAAGGGTTTC GCCTCGTGCC CTTTTTGACT GAGCTGAGGG CAGTGATGGA CTGGGTGTGG 
ACGGACACAA CTTTGAGCCT GTCCAGCTGG ATCTGTGTGG AGGACATCTA TGCTCACATA 
TTCATCCTGA AGTGTTGGCG GGAGTCGGAG AAGAGATACC CTCAGCCACG GGGCCAGAAG 
AAGAAGAAAG TGGTGAAGTA TGGCATGGGA GGAATGATCA TCGTCCTGCT CATCTGCATT 
GTCTGGTTTC CTCTTCTCTT CATGTCTTTG ATCAAATCTG TGGCTGGGGT CATCAACCAG 
CCCCTGGACG TCTCCGTCAC AATTACCCTG GGAGGGTATC AGCCTATTTT CACAATGAGT 
GCCCAACAAA GCCAGTTGAA AGTTATGGAC CAGCAGAGCT TTAACAAATT TATACAAGCT 
TTTTCTAGGG ACACCGGTGC TATGCAATTT CTGGAAAATT ATGAAAAAGA AGACATAACA 
GTAGCAGAAC TGGAAGGAAA CTCAAATTCT TTGTGGACCA TCAGCCCACC CAGTAAGCAG 
AAAATGATAC ACGAACTCCT GGACCCCAAT AGTAGCTTCT CTGTTGTTTT TTCATGGAGT 
ATTCAGAGAA ACTTAAGTCT GGGTGCAAAA TCGGAAATAG CAACAGATAA GCTTTCTTTT 
CCTCTTAAAA ATATTACTCG AAAGAATATC GCTAAAATGA TAGCAGGCAA CAGCACAGAA 
AGTTCAAAAA CACCAGTGAC CATAGAAAAG ATTTATCCAT ATTATGTGAA AGCACCTAGT 
GATTCTAACT CAAAACCTAT AAAGCAACTT TTATCTGAAA ATAATTTCAT GGATATTACC 
ATCATTTTGT CCAGAGACAA TACAACTAAA TATAACAGTG AGTGGTGGGT TCTCAACCTG 
ACTGGAAACA GAATATACAA TCCGAACTCT CAGGCCCTGG AACTGGTGGT CTTCAATGAC 
AAAGTCAGTC CCCCAAGTCT GGGGTTCCTG GCTGGCTATG GTATTATGGG ATTATATGCT 
TCAGTTGTCC TTGTGATTGG GAAATTTGTC CGTGAATTCT TCAGTGGGAT TTCTCACTCC 
ATCATGTTTG AAGAGCTTCC AAATGTGGAT CGAATTTTGA AGTTGTGCAC AGATATTTTT 
TTAGTTCGAG AGACAGGAGA ACTGGAGCTA GAAGAAGATC TCTATGCCAA ATTAATATTC 
CTATATCGCT CACCAGAGAC AATGATCAAA TGGACTAGAG AAAAAACAAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIEZO2 ( XM_011525725.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524027.1 Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X4, mRNA.

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CloneID	OHu71133
Clone ID Related Accession (Same CDS sequence)	XM_011525725.3 , XM_011525725.2
Accession Version	XM_011525725.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	piezo-type mechanosensitive ion channel component 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011525725.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG AAGTGGTGTG CGGGCTCATC TTCAGGCTGC TGCTGCCCAT CTGCCTGGCA 
GTAGCATGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC 
ATTCCTCTGT TCTCAGAACC AACAAAAACG ACGATGCAAG GACATACGGG ACGGTTATTA 
AAGTCTCTGT GCTTCATCAG TCTTTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG 
TTGGTGAGCC TTGAAGCTCA ACATCGTATT GCACCTGGCT ACAACTGCTC AACATGGGAA 
AAGACATTCC GGCAGATCGG CTTTGAAAGC TTAAAGGGAG CTGATGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCCTCTGT 
AGAAACATTG TTCAGAAACC TGTGACAGAC GAAGCAGCAC AGAGTAACCC GGAGTTTGAA 
AATGAAGAAT TGGCTGAAGG AGAAAAAATT GATTCAGAAG AGGCACTGAT CTATGAAGAG 
GATTTCAATG GAGGAGATGG TGTTGAAGGC GAGTTGGAAG AAAGCACGAA GTTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCCTCTAAG CTCAAGGAGT TCATTGGCAA CATGATCACC 
ACTGCTGGGA AAGTCGTTGT TACCATCTTA CTGGGCTCCT CGGGCATGAT GTTGCCGTCT 
TTGACATCAT CTGTGTATTT TTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACTGCT 
GGACATTTGA TTGGACTTTA TTTATACCAG TTCCAATTCT TTCAAGAGGC AGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAGTCAGTAA TTCAAACGGA CTGTTCAAGT 
ACTTGGAAGA TCATAGTGAA CCCGGACCTG TCGTGGTACC ACCACGCCAA CCCTATCCTC 
CTGCTGGTGA TGTACTACAC TCTGGCCACT CTGATCCGCA TCTGGCTGCA AGAGCCCCTT 
GTGCAGGATG AGGGGACCAA AGAAGAGGAC AAAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACAGCGGGGA GGAGGCGGAG CCTGTGGTAC GCAACCCATT ACCCCACTGA TGAGAGAAAA 
CTTTTATCCA TGACCCAGGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC 
GGCAACCCCG TGGATTACCA CACCATCCAC CCAAGCCTGC CCATGGAGAA CGGCCCTGGC 
AAAGCCGACC TCTACTCCAC CCCTCAGTAC CGGTGGGAGC CCTCTGATGA ATCCTCAGAA 
AAGCGAGAGG AGGAAGAGGA AGAGAAAGAA GAATTTGAAG AAGAAAGGAG CCGTGAGGAA 
AAAAGAAGTA TCAAAGTTCA TGCCATGGTC TCCGTATTCC AATTTATTAT GAAACAAAGT 
TACATCTGTG CCCTCATAGC TATGATGGCC TGGAGCATCA CCTATCACAG CTGGCTGACC 
TTCGTGCTGC TGATCTGGTC GTGCACTCTT TGGATGATTC GCAACAGAAG AAAATATGCC 
ATGATCAGCT CTCCCTTCAT GGTGGTTTAT GGAAACCTAT TGTTGATATT ACAGTATATA 
TGGAGTTTTG AACTTCCTGA AATTAAAAAA GTTCCAGGAT TTTTAGAAAA GAAAGAGCCA 
GGAGAACTTG CTTCCAAAAT CCTTTTCACC ATTACTTTTT GGCTACTGCT GAGGCAGCAC 
CTCACAGAGC AAAAAGCTCT GCAAGAAAAG GAAGCTCTTT TATCGGAAGT CAAAATTGGC 
AGTCAGGAAA ATGAAGAAAA AGATGAGGAA CTTCAAGATA TACAAGTGGA AGGAGAGCCC 
AAAGAGGAGG AAGAAGAGGA AGCGAAGGAA GAGAAGCAAG AGAGAAAGAA GGTAGAGCAA 
GAGGAAGCTG AAGAAGAAGA TGAGCAGGAC ATCATGAAAG TCCTGGGCAA TCTGGTGGTG 
GCCATGTTCA TCAAGTACTG GATCTACGTC TGCGGAGGCA TGTTCTTCTT CGTCAGCTTC 
GAGGGTAAAA TCGTAATGTA CAAAATCATC TACATGGTGC TGTTCCTGTT CTGTGTGGCC 
CTATACCAGG TGCACTATGA ATGGTGGAGG AAAATTCTAA AATATTTTTG GATGTCAGTG 
GTTATTTACA CTATGCTGGT GCTTATCTTT ATATACACAT ATCAGTTTGA GAACTTCCCA 
GGCCTGTGGC AAAATATGAC TGGACTGAAA AAAGAAAAGC TTGAGGATCT TGGCTTAAAG 
CAGTTTACTG TGGCTGAACT ATTCACTCGC ATATTCATCC CAACCTCCTT TCTGCTGGTG 
TGCATTTTAC ACCTGCACTA CTTCCATGAC CGGTTCCTTG AACTCACAGA CCTCAAGTCC 
ATTCCCAGCA AAGAAGACAA CACCATCTAC AGCCATGCCA AAGTCAATGG TCGCGTATAT 
CTAATAATAA ATAGACTGGC CCACCCGGAA GGAAGCCTCC CGGACCTCAC CATGATGCAT 
CTGACTGCCA GCCTGGAGAA GCCGGAGGTG AGGAAGTTGG CTGAGCCTGG GGAGGAGAAG 
CTTGAGGGCT ACTCTGAAAA AGCCCAGAAG GGTGATCTTG GGAAAGACAG CGAGGAGTCA 
GAGGAGGACG GAGAGGAAGA GGAGGAATCC GAGGAGGAGG AAGAAACATC AGACTTAAGG 
AACAAATGGC ACCTGGTGAT TGACCGCCTC ACTGTGCTCT TCTTAAAATT CCTGGAGTAT 
TTTCACAAGC TGCAGGTGTT CATGTGGTGG ATTTTGGAGT TGCACATCAT CAAAATCGTT 
TCCTCTTACA TTATCTGGGT TTCTGTGAAA GAGGTGTCTC TGTTCAACTA TGTATTTTTG 
ATTTCTTGGG CTTTTGCTCT GCCGTACGCC AAGCTGCGCC GTCTGGCTTC AAGTGTCTGC 
ACAGTCTGGA CGTGTGTGAT CATCGTCTGC AAAATGTTGT ACCAGCTCCA AACCATTAAG 
CCTGAGAACT TCTCTGTTAA CTGTTCCTTG CCAAATGAAA ATCAAACAAA CATCCCCTTT 
AATGAGTTGA ACAAGTCTCT GCTCTACAGC GCTCCTATCG ATCCTACAGA GTGGGTCGGC 
CTGCGGAAGT CTTCGCCTCT GCTAGTCTAC CTGAGGAATA ACCTCCTGAT GCTGGCTATC 
CTGGCCTTTG AAGTCACCAT TTACCGCCAT CAGGAATACT ATCGAGGTCG AAATAACCTG 
ACGGCCCCTG TGTCTAGAAC TATCTTTCAT GACATTACAA GACTACATCT AGATGATGGA 
CTTATTAATT GTGCCAAATA TTTCATTAAT TACTTCTTTT ACAAGTTTGG TCTGGAGACC 
TGTTTCCTAA TGTCAGTTAA CGTCATTGGC CAGCGAATGG ATTTCTATGC CATGATCCAC 
GCCTGCTGGC TGATCGCTGT CTTATATAGA CGCAGAAGGA AAGCCATCGC AGAGATCTGG 
CCCAAGTACT GCTGCTTCCT GGCATGCATC ATCACCTTCC AGTATTTCAT CTGCATTGGC 
ATCCCACCTG CTCCTTGCCG AGATTACCCG TGGAGATTCA AGGGTGCCAG CTTCAATGAC 
AACATCATAA AGTGGCTGTA CTTCCCAGAT TTCATTGTGC GGCCCAACCC TGTGTTTCTC 
GTCTATGACT TCATGCTGCT TCTGTGTGCC TCCTTACAAC GGCAGATTTT TGAGGATGAG 
AACAAGGCTG CAGTGCGAAT CATGGCAGGT GACAATGTCG AGATCTGCAT GAACCTTGAT 
GCGGCCTCCT TCAGCCAACA TAACCCTGTG CCAGATTTTA TTCACTGCAG ATCTTACTTA 
GACATGTCCA AAGTGATCAT CTTCAGCTAC CTCTTCTGGT TTGTGCTCAC CATCATCTTC 
ATCACTGGGA CCACCAGGAT CAGCATCTTT TGCATGGGGT ACCTGGTGGC CTGTTTCTAC 
TTCCTGCTCT TTGGGGGCGA TTTGCTGTTG AAACCCATCA AGAGCATCCT GCGCTACTGG 
GACTGGCTGA TCGCATACAA CGTTTTTGTG ATTACGATGA AAAATATCCT GTCAATAGGA 
GCATGTGGAT ACATTGGAAC ATTGGTGCAC AATAGTTGTT GGTTGATCCA GGCTTTCAGC 
CTGGCCTGCA CAGTCAAAGG CTATCAAATG CCTGCTGCTA ATTCACCCTG TACACTTCCC 
AGTGGGGAAG CAGGAATCAT TTGGGACAGC ATATGTTTTG CCTTCCTCCT GCTGCAAAGA 
AGAGTTTTCA TGAGTTATTA TTTTCTACAT GTTGTGGCTG ATATAAAAGC TTCCCAGATT 
CTGGCATCAA GAGGAGCTGA ACTTTTCCAG GCCACAATTG TAAAAGCTGT AAAGGCAAGA 
ATTGAGGAAG AGAAGAAGTC CATGGACCAG CTGAAGCGAC AGATGGATCG CATCAAGGCC 
AGGCAACAGA AATATAAAAA GGGTAAGGAG AGGATGCTGA GCTTGACCCA GGAGCCAGGG 
GAAGGCCAGG ACATGCAAAA ACTCTCTGAA GAGGATGATG AAAGAGAAGC AGACAAACAG 
AAAGCCAAGG GCAAAAAAAA GCAGTGGTGG CGGCCTTGGG TTGATCATGC TTCCATGGTC 
AGGAGTGGAG ATTATTATTT GTTTGAAACG GATAGTGAAG AGGAGGAAGA GGAAGAATTA 
AAGAAGGAAG ATGAAGAACC TCCACGAAGG TCAGCATTCC AGTTTGTTTA TCAAGCCTGG 
ATTACTGATC CTAAAACAGC ACTCCGACAA AGACACAAAG AGAAAAAAAG GTCTGCAAGA 
GAAGAACGGA AACGAAGGCG GAAAGGATCC AAGGAGGGTC CTGTGGAATG GGAAGACCGG 
GAGGATGAAC CAATCAAAAA GAAATCCGAT GGACCAGATA ATATCATCAA GAGGATATTT 
AATATTTTGA AATTTACCTG GGTCCTATTT CTGGCAACAG TGGACAGTTT CACTACTTGG 
CTTAACTCCA TTTCAAGGGA GCATATTGAT ATATCTACAG TTCTGAGAAT TGAACGATGC 
ATGCTGACCA GAGAAATTAA GAAGGGCAAT GTTCCAACTC GGGAGAGCAT CCACATGTAC 
TATCAGAACC ACATCATGAA CCTTTCCAGA GAGTCGGGAC TGGACACCAT TGACGAGCAT 
CCCGGAGCTG CTTCAGGTGC ACAGACAGCC CACAGGATGG ATAGTTTAGA TTCACATGAC 
AGTATCTCCA GCGAGCCCAC GCAGTGTACC ATGCTGTACT CACGCCAGGG GACCACTGAG 
ACCATCGAGG AGGTGGAGGC TGAGCAGGAG GAGGAGGCAG GGAGCACGGC GCCTGAGCCC 
AGGGAGGCCA AGGAGTACGA GGCCACTGGG TACGATGTGG GAGCCATGGG TGCCGAGGAG 
GCCAGCCTCA CCCCAGAGGA AGAGCTGACA CAGTTCTCCA CCTTGGACGG GGATGTGGAG 
GCCCCACCCT CCTACAGCAA GGCTGTGAGC TTCGAGCATC TGTCCTTCGG CTCGCAGGAC 
GACTCTGCAG GCAAGAACCG TATGGCAGTC AGCCCGGACG ACAGCCGCAC CGACAAGCTG 
GGGTCCAGCA TCTTACCTCC CCTGACCCAT GAGCTGACGG CCAGCGAGCT GCTGCTGAAA 
AAGATGTTTC ACGACGATGA GCTTGAAGAG TCAGAGAAAT TCTACGTGGG GCAGCCCCGA 
TTTCTGCTGC TCTTCTATGC CATGTACAAT ACCCTGGTGG CCCGCTCGGA GATGGTGTGC 
TACTTCGTGA TCATCCTCAA CCACATGGTC TCTGCCTCCA TGATCACGCT CCTGCTTCCC 
ATCCTCATCT TCCTCTGGGC CATGTTGTCC GTCCCCAGGC CCAGCCGCCG GTTCTGGATG 
ATGGCCATCG TCTATACTGA GGTGGCAATT GTAGTCAAGT ATTTCTTCCA ATTTGGGTTC 
TTTCCCTGGA ATAAGAATGT GGAGGTGAAC AAAGATAAAC CGTATCACCC CCCAAACATC 
ATAGGAGTGG AAAAGAAGGA AGGTTATGTT CTCTATGACC TCATCCAGCT CCTGGCTCTG 
TTCTTTCATC GATCAATTTT GAAGTGCCAT GGCTTATGGG ATGAAGATGA CATGACTGAA 
AGTGGCATGG CCAGGGAGGA ATCAGATGAT GAGCTCTCCC TCGGTCATGG CAGGAGGGAC 
TCCTCCGATT CTCTCAAGTC CATCAACCTG GCCGCGTCTG TGGAGTCAGT GCATGTGACC 
TTCCCGGAGC AGCAGACAGC TGTCCGGAGG AAGCGCTCCG GCAGCAGCTC CGAGCCATCC 
CAGAGATCCA GCTTTTCTTC AAACAGATCC CAAAGAGGCA GCACAAGCAC CCGAAACAGC 
AGTCAAAAAG GAAGCAGTGT TTTGAGTATT AAGCAAAAAG GCAAAAGGGA ACTTTATATG 
GAAAAGCTTC AAGAACATTT AATCAAAGCA AAAGCCTTTA CCATAAAGAA GACGCTGGAG 
ATCTATGTGC CCATCAAACA GTTCTTTTAC AACCTCATCC ACCCGGAGTA TAGCGCCGTG 
ACTGACGTGT ATGTACTCAT GTTCCTGGCT GACACTGTGG ACTTCATCAT CATTGTCTTC 
GGCTTTTGGG CCTTTGGGAA ACACTCAGCA GCTGCAGACA TCACCTCTTC ACTGTCAGAG 
GACCAGGTCC CGGGGCCGTT TTTGGTGATG GTCCTCATTC AGTTTGGAAC CATGGTGGTG 
GACCGAGCCC TCTACCTCAG GAAGACTGTA CTGGGAAAGG TCATCTTCCA GGTCATTCTT 
GTGTTCGGAA TTCACTTCTG GATGTTCTTC ATCTTACCTG GTGTGACTGA GAGGAAATTC 
AGCCAGAACC TGGTTGCCCA GCTTTGGTAC TTTGTGAAAT GTGTTTACTT CGGGTTGTCT 
GCTTACCAGA TCCGTTGTGG CTACCCAACG CGAGTCCTGG GGAACTTCCT CACCAAGAGC 
TACAATTACG TCAACCTCTT CTTATTCCAA GGGTTTCGCC TCGTGCCCTT TTTGACTGAG 
CTGAGGGCAG TGATGGACTG GGTGTGGACG GACACAACTT TGAGCCTGTC CAGCTGGATC 
TGTGTGGAGG ACATCTATGC TCACATATTC ATCCTGAAGT GTTGGCGGGA GTCGGAGAAG 
AGATACCCTC AGCCACGGGG CCAGAAGAAG AAGAAAGTGG TGAAGTATGG CATGGGAGGA 
ATGATCATCG TCCTGCTCAT CTGCATTGTC TGGTTTCCTC TTCTCTTCAT GTCTTTGATC 
AAATCTGTGG CTGGGGTCAT CAACCAGCCC CTGGACGTCT CCGTCACAAT TACCCTGGGA 
GGGTATCAGC CTATTTTCAC AATGAGTGCC CAACAAAGCC AGTTGAAAGT TATGGACCAG 
CAGAGCTTTA ACAAATTTAT ACAAGCTTTT TCTAGGGACA CCGGTGCTAT GCAATTTCTG 
GAAAATTATG AAAAAGAAGA CATAACAGTA GCAGAACTGG AAGGAAACTC AAATTCTTTG 
TGGACCATCA GCCCACCCAG TAAGCAGAAA ATGATACACG AACTCCTGGA CCCCAATAGT 
AGCTTCTCTG TTGTTTTTTC ATGGAGTATT CAGAGAAACT TAAGTCTGGG TGCAAAATCG 
GAAATAGCAA CAGATAAGCT TTCTTTTCCT CTTAAAAATA TTACTCGAAA GAATATCGCT 
AAAATGATAG CAGGCAACAG CACAGAAAGT TCAAAAACAC CAGTGACCAT AGAAAAGATT 
TATCCATATT ATGTGAAAGC ACCTAGTGAT TCTAACTCAA AACCTATAAA GCAACTTTTA 
TCTGAAAATA ATTTCATGGA TATTACCATC ATTTTGTCCA GAGACAATAC AACTAAATAT 
AACAGTGAGT GGTGGGTTCT CAACCTGACT GGAAACAGAA TATACAATCC GAACTCTCAG 
GCCCTGGAAC TGGTGGTCTT CAATGACAAA GTCAGTCCCC CAAGTCTGGG GTTCCTGGCT 
GGCTATGGTA TTATGGGATT ATATGCTTCA GTTGTCCTTG TGATTGGGAA ATTTGTCCGT 
GAATTCTTCA GTGGGATTTC TCACTCCATC ATGTTTGAAG AGCTTCCAAA TGTGGATCGA 
ATTTTGAAGT TGTGCACAGA TATTTTTTTA GTTCGAGAGA CAGGAGAACT GGAGCTAGAA 
GAAGATCTCT ATGCCAAATT AATATTCCTA TATCGCTCAC CAGAGACAAT GATCAAATGG 
ACTAGAGAAA AAACAAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524027.1
CDS	998..9298
Translation	MASEVVCGLIFRLLLPICLAVACAFRYNGLSFVYLIYLLLIPLFSEPTKTTMQGHTGRLLKSLCFISLSFLLLHIIFHITLVSLEAQHRIAPGYNCSTWEKTFRQIGFESLKGADAGNGIRVFVPDIGMFIASLTIWLLCRNIVQKPVTDEAAQSNPEFENEELAEGEKIDSEEALIYEEDFNGGDGVEGELEESTKLKMFRRLASVASKLKEFIGNMITTAGKVVVTILLGSSGMMLPSLTSSVYFFVFLGLCTWWSWCRTFDPLLFSCLCVLLAIFTAGHLIGLYLYQFQFFQEAVPPNDYYARLFGIKSVIQTDCSSTWKIIVNPDLSWYHHANPILLLVMYYTLATLIRIWLQEPLVQDEGTKEEDKALACSPIQITAGRRRSLWYATHYPTDERKLLSMTQDDYKPSDGLLVTVNGNPVDYHTIHPSLPMENGPGKADLYSTPQYRWEPSDESSEKREEEEEEKEEFEEERSREEKRSIKVHAMVSVFQFIMKQSYICALIAMMAWSITYHSWLTFVLLIWSCTLWMIRNRRKYAMISSPFMVVYGNLLLILQYIWSFELPEIKKVPGFLEKKEPGELASKILFTITFWLLLRQHLTEQKALQEKEALLSEVKIGSQENEEKDEELQDIQVEGEPKEEEEEEAKEEKQERKKVEQEEAEEEDEQDIMKVLGNLVVAMFIKYWIYVCGGMFFFVSFEGKIVMYKIIYMVLFLFCVALYQVHYEWWRKILKYFWMSVVIYTMLVLIFIYTYQFENFPGLWQNMTGLKKEKLEDLGLKQFTVAELFTRIFIPTSFLLVCILHLHYFHDRFLELTDLKSIPSKEDNTIYSHAKVNGRVYLIINRLAHPEGSLPDLTMMHLTASLEKPEVRKLAEPGEEKLEGYSEKAQKGDLGKDSEESEEDGEEEEESEEEEETSDLRNKWHLVIDRLTVLFLKFLEYFHKLQVFMWWILELHIIKIVSSYIIWVSVKEVSLFNYVFLISWAFALPYAKLRRLASSVCTVWTCVIIVCKMLYQLQTIKPENFSVNCSLPNENQTNIPFNELNKSLLYSAPIDPTEWVGLRKSSPLLVYLRNNLLMLAILAFEVTIYRHQEYYRGRNNLTAPVSRTIFHDITRLHLDDGLINCAKYFINYFFYKFGLETCFLMSVNVIGQRMDFYAMIHACWLIAVLYRRRRKAIAEIWPKYCCFLACIITFQYFICIGIPPAPCRDYPWRFKGASFNDNIIKWLYFPDFIVRPNPVFLVYDFMLLLCASLQRQIFEDENKAAVRIMAGDNVEICMNLDAASFSQHNPVPDFIHCRSYLDMSKVIIFSYLFWFVLTIIFITGTTRISIFCMGYLVACFYFLLFGGDLLLKPIKSILRYWDWLIAYNVFVITMKNILSIGACGYIGTLVHNSCWLIQAFSLACTVKGYQMPAANSPCTLPSGEAGIIWDSICFAFLLLQRRVFMSYYFLHVVADIKASQILASRGAELFQATIVKAVKARIEEEKKSMDQLKRQMDRIKARQQKYKKGKERMLSLTQEPGEGQDMQKLSEEDDEREADKQKAKGKKKQWWRPWVDHASMVRSGDYYLFETDSEEEEEEELKKEDEEPPRRSAFQFVYQAWITDPKTALRQRHKEKKRSAREERKRRRKGSKEGPVEWEDREDEPIKKKSDGPDNIIKRIFNILKFTWVLFLATVDSFTTWLNSISREHIDISTVLRIERCMLTREIKKGNVPTRESIHMYYQNHIMNLSRESGLDTIDEHPGAASGAQTAHRMDSLDSHDSISSEPTQCTMLYSRQGTTETIEEVEAEQEEEAGSTAPEPREAKEYEATGYDVGAMGAEEASLTPEEELTQFSTLDGDVEAPPSYSKAVSFEHLSFGSQDDSAGKNRMAVSPDDSRTDKLGSSILPPLTHELTASELLLKKMFHDDELEESEKFYVGQPRFLLLFYAMYNTLVARSEMVCYFVIILNHMVSASMITLLLPILIFLWAMLSVPRPSRRFWMMAIVYTEVAIVVKYFFQFGFFPWNKNVEVNKDKPYHPPNIIGVEKKEGYVLYDLIQLLALFFHRSILKCHGLWDEDDMTESGMAREESDDELSLGHGRRDSSDSLKSINLAASVESVHVTFPEQQTAVRRKRSGSSSEPSQRSSFSSNRSQRGSTSTRNSSQKGSSVLSIKQKGKRELYMEKLQEHLIKAKAFTIKKTLEIYVPIKQFFYNLIHPEYSAVTDVYVLMFLADTVDFIIIVFGFWAFGKHSAAADITSSLSEDQVPGPFLVMVLIQFGTMVVDRALYLRKTVLGKVIFQVILVFGIHFWMFFILPGVTERKFSQNLVAQLWYFVKCVYFGLSAYQIRCGYPTRVLGNFLTKSYNYVNLFLFQGFRLVPFLTELRAVMDWVWTDTTLSLSSWICVEDIYAHIFILKCWRESEKRYPQPRGQKKKKVVKYGMGGMIIVLLICIVWFPLLFMSLIKSVAGVINQPLDVSVTITLGGYQPIFTMSAQQSQLKVMDQQSFNKFIQAFSRDTGAMQFLENYEKEDITVAELEGNSNSLWTISPPSKQKMIHELLDPNSSFSVVFSWSIQRNLSLGAKSEIATDKLSFPLKNITRKNIAKMIAGNSTESSKTPVTIEKIYPYYVKAPSDSNSKPIKQLLSENNFMDITIILSRDNTTKYNSEWWVLNLTGNRIYNPNSQALELVVFNDKVSPPSLGFLAGYGIMGLYASVVLVIGKFVREFFSGISHSIMFEELPNVDRILKLCTDIFLVRETGELELEEDLYAKLIFLYRSPETMIKWTREKTN

Target ORF information:

RefSeq Version	XM_011525725.3
Organism	Homo sapiens(human)
Definition	Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525725.3

ORF Insert Sequence:

ATGGCCTCAG AAGTGGTGTG CGGGCTCATC TTCAGGCTGC TGCTGCCCAT CTGCCTGGCA 
GTAGCATGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC 
ATTCCTCTGT TCTCAGAACC AACAAAAACG ACGATGCAAG GACATACGGG ACGGTTATTA 
AAGTCTCTGT GCTTCATCAG TCTTTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG 
TTGGTGAGCC TTGAAGCTCA ACATCGTATT GCACCTGGCT ACAACTGCTC AACATGGGAA 
AAGACATTCC GGCAGATCGG CTTTGAAAGC TTAAAGGGAG CTGATGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCCTCTGT 
AGAAACATTG TTCAGAAACC TGTGACAGAC GAAGCAGCAC AGAGTAACCC GGAGTTTGAA 
AATGAAGAAT TGGCTGAAGG AGAAAAAATT GATTCAGAAG AGGCACTGAT CTATGAAGAG 
GATTTCAATG GAGGAGATGG TGTTGAAGGC GAGTTGGAAG AAAGCACGAA GTTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCCTCTAAG CTCAAGGAGT TCATTGGCAA CATGATCACC 
ACTGCTGGGA AAGTCGTTGT TACCATCTTA CTGGGCTCCT CGGGCATGAT GTTGCCGTCT 
TTGACATCAT CTGTGTATTT TTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACTGCT 
GGACATTTGA TTGGACTTTA TTTATACCAG TTCCAATTCT TTCAAGAGGC AGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAGTCAGTAA TTCAAACGGA CTGTTCAAGT 
ACTTGGAAGA TCATAGTGAA CCCGGACCTG TCGTGGTACC ACCACGCCAA CCCTATCCTC 
CTGCTGGTGA TGTACTACAC TCTGGCCACT CTGATCCGCA TCTGGCTGCA AGAGCCCCTT 
GTGCAGGATG AGGGGACCAA AGAAGAGGAC AAAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACAGCGGGGA GGAGGCGGAG CCTGTGGTAC GCAACCCATT ACCCCACTGA TGAGAGAAAA 
CTTTTATCCA TGACCCAGGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC 
GGCAACCCCG TGGATTACCA CACCATCCAC CCAAGCCTGC CCATGGAGAA CGGCCCTGGC 
AAAGCCGACC TCTACTCCAC CCCTCAGTAC CGGTGGGAGC CCTCTGATGA ATCCTCAGAA 
AAGCGAGAGG AGGAAGAGGA AGAGAAAGAA GAATTTGAAG AAGAAAGGAG CCGTGAGGAA 
AAAAGAAGTA TCAAAGTTCA TGCCATGGTC TCCGTATTCC AATTTATTAT GAAACAAAGT 
TACATCTGTG CCCTCATAGC TATGATGGCC TGGAGCATCA CCTATCACAG CTGGCTGACC 
TTCGTGCTGC TGATCTGGTC GTGCACTCTT TGGATGATTC GCAACAGAAG AAAATATGCC 
ATGATCAGCT CTCCCTTCAT GGTGGTTTAT GGAAACCTAT TGTTGATATT ACAGTATATA 
TGGAGTTTTG AACTTCCTGA AATTAAAAAA GTTCCAGGAT TTTTAGAAAA GAAAGAGCCA 
GGAGAACTTG CTTCCAAAAT CCTTTTCACC ATTACTTTTT GGCTACTGCT GAGGCAGCAC 
CTCACAGAGC AAAAAGCTCT GCAAGAAAAG GAAGCTCTTT TATCGGAAGT CAAAATTGGC 
AGTCAGGAAA ATGAAGAAAA AGATGAGGAA CTTCAAGATA TACAAGTGGA AGGAGAGCCC 
AAAGAGGAGG AAGAAGAGGA AGCGAAGGAA GAGAAGCAAG AGAGAAAGAA GGTAGAGCAA 
GAGGAAGCTG AAGAAGAAGA TGAGCAGGAC ATCATGAAAG TCCTGGGCAA TCTGGTGGTG 
GCCATGTTCA TCAAGTACTG GATCTACGTC TGCGGAGGCA TGTTCTTCTT CGTCAGCTTC 
GAGGGTAAAA TCGTAATGTA CAAAATCATC TACATGGTGC TGTTCCTGTT CTGTGTGGCC 
CTATACCAGG TGCACTATGA ATGGTGGAGG AAAATTCTAA AATATTTTTG GATGTCAGTG 
GTTATTTACA CTATGCTGGT GCTTATCTTT ATATACACAT ATCAGTTTGA GAACTTCCCA 
GGCCTGTGGC AAAATATGAC TGGACTGAAA AAAGAAAAGC TTGAGGATCT TGGCTTAAAG 
CAGTTTACTG TGGCTGAACT ATTCACTCGC ATATTCATCC CAACCTCCTT TCTGCTGGTG 
TGCATTTTAC ACCTGCACTA CTTCCATGAC CGGTTCCTTG AACTCACAGA CCTCAAGTCC 
ATTCCCAGCA AAGAAGACAA CACCATCTAC AGCCATGCCA AAGTCAATGG TCGCGTATAT 
CTAATAATAA ATAGACTGGC CCACCCGGAA GGAAGCCTCC CGGACCTCAC CATGATGCAT 
CTGACTGCCA GCCTGGAGAA GCCGGAGGTG AGGAAGTTGG CTGAGCCTGG GGAGGAGAAG 
CTTGAGGGCT ACTCTGAAAA AGCCCAGAAG GGTGATCTTG GGAAAGACAG CGAGGAGTCA 
GAGGAGGACG GAGAGGAAGA GGAGGAATCC GAGGAGGAGG AAGAAACATC AGACTTAAGG 
AACAAATGGC ACCTGGTGAT TGACCGCCTC ACTGTGCTCT TCTTAAAATT CCTGGAGTAT 
TTTCACAAGC TGCAGGTGTT CATGTGGTGG ATTTTGGAGT TGCACATCAT CAAAATCGTT 
TCCTCTTACA TTATCTGGGT TTCTGTGAAA GAGGTGTCTC TGTTCAACTA TGTATTTTTG 
ATTTCTTGGG CTTTTGCTCT GCCGTACGCC AAGCTGCGCC GTCTGGCTTC AAGTGTCTGC 
ACAGTCTGGA CGTGTGTGAT CATCGTCTGC AAAATGTTGT ACCAGCTCCA AACCATTAAG 
CCTGAGAACT TCTCTGTTAA CTGTTCCTTG CCAAATGAAA ATCAAACAAA CATCCCCTTT 
AATGAGTTGA ACAAGTCTCT GCTCTACAGC GCTCCTATCG ATCCTACAGA GTGGGTCGGC 
CTGCGGAAGT CTTCGCCTCT GCTAGTCTAC CTGAGGAATA ACCTCCTGAT GCTGGCTATC 
CTGGCCTTTG AAGTCACCAT TTACCGCCAT CAGGAATACT ATCGAGGTCG AAATAACCTG 
ACGGCCCCTG TGTCTAGAAC TATCTTTCAT GACATTACAA GACTACATCT AGATGATGGA 
CTTATTAATT GTGCCAAATA TTTCATTAAT TACTTCTTTT ACAAGTTTGG TCTGGAGACC 
TGTTTCCTAA TGTCAGTTAA CGTCATTGGC CAGCGAATGG ATTTCTATGC CATGATCCAC 
GCCTGCTGGC TGATCGCTGT CTTATATAGA CGCAGAAGGA AAGCCATCGC AGAGATCTGG 
CCCAAGTACT GCTGCTTCCT GGCATGCATC ATCACCTTCC AGTATTTCAT CTGCATTGGC 
ATCCCACCTG CTCCTTGCCG AGATTACCCG TGGAGATTCA AGGGTGCCAG CTTCAATGAC 
AACATCATAA AGTGGCTGTA CTTCCCAGAT TTCATTGTGC GGCCCAACCC TGTGTTTCTC 
GTCTATGACT TCATGCTGCT TCTGTGTGCC TCCTTACAAC GGCAGATTTT TGAGGATGAG 
AACAAGGCTG CAGTGCGAAT CATGGCAGGT GACAATGTCG AGATCTGCAT GAACCTTGAT 
GCGGCCTCCT TCAGCCAACA TAACCCTGTG CCAGATTTTA TTCACTGCAG ATCTTACTTA 
GACATGTCCA AAGTGATCAT CTTCAGCTAC CTCTTCTGGT TTGTGCTCAC CATCATCTTC 
ATCACTGGGA CCACCAGGAT CAGCATCTTT TGCATGGGGT ACCTGGTGGC CTGTTTCTAC 
TTCCTGCTCT TTGGGGGCGA TTTGCTGTTG AAACCCATCA AGAGCATCCT GCGCTACTGG 
GACTGGCTGA TCGCATACAA CGTTTTTGTG ATTACGATGA AAAATATCCT GTCAATAGGA 
GCATGTGGAT ACATTGGAAC ATTGGTGCAC AATAGTTGTT GGTTGATCCA GGCTTTCAGC 
CTGGCCTGCA CAGTCAAAGG CTATCAAATG CCTGCTGCTA ATTCACCCTG TACACTTCCC 
AGTGGGGAAG CAGGAATCAT TTGGGACAGC ATATGTTTTG CCTTCCTCCT GCTGCAAAGA 
AGAGTTTTCA TGAGTTATTA TTTTCTACAT GTTGTGGCTG ATATAAAAGC TTCCCAGATT 
CTGGCATCAA GAGGAGCTGA ACTTTTCCAG GCCACAATTG TAAAAGCTGT AAAGGCAAGA 
ATTGAGGAAG AGAAGAAGTC CATGGACCAG CTGAAGCGAC AGATGGATCG CATCAAGGCC 
AGGCAACAGA AATATAAAAA GGGTAAGGAG AGGATGCTGA GCTTGACCCA GGAGCCAGGG 
GAAGGCCAGG ACATGCAAAA ACTCTCTGAA GAGGATGATG AAAGAGAAGC AGACAAACAG 
AAAGCCAAGG GCAAAAAAAA GCAGTGGTGG CGGCCTTGGG TTGATCATGC TTCCATGGTC 
AGGAGTGGAG ATTATTATTT GTTTGAAACG GATAGTGAAG AGGAGGAAGA GGAAGAATTA 
AAGAAGGAAG ATGAAGAACC TCCACGAAGG TCAGCATTCC AGTTTGTTTA TCAAGCCTGG 
ATTACTGATC CTAAAACAGC ACTCCGACAA AGACACAAAG AGAAAAAAAG GTCTGCAAGA 
GAAGAACGGA AACGAAGGCG GAAAGGATCC AAGGAGGGTC CTGTGGAATG GGAAGACCGG 
GAGGATGAAC CAATCAAAAA GAAATCCGAT GGACCAGATA ATATCATCAA GAGGATATTT 
AATATTTTGA AATTTACCTG GGTCCTATTT CTGGCAACAG TGGACAGTTT CACTACTTGG 
CTTAACTCCA TTTCAAGGGA GCATATTGAT ATATCTACAG TTCTGAGAAT TGAACGATGC 
ATGCTGACCA GAGAAATTAA GAAGGGCAAT GTTCCAACTC GGGAGAGCAT CCACATGTAC 
TATCAGAACC ACATCATGAA CCTTTCCAGA GAGTCGGGAC TGGACACCAT TGACGAGCAT 
CCCGGAGCTG CTTCAGGTGC ACAGACAGCC CACAGGATGG ATAGTTTAGA TTCACATGAC 
AGTATCTCCA GCGAGCCCAC GCAGTGTACC ATGCTGTACT CACGCCAGGG GACCACTGAG 
ACCATCGAGG AGGTGGAGGC TGAGCAGGAG GAGGAGGCAG GGAGCACGGC GCCTGAGCCC 
AGGGAGGCCA AGGAGTACGA GGCCACTGGG TACGATGTGG GAGCCATGGG TGCCGAGGAG 
GCCAGCCTCA CCCCAGAGGA AGAGCTGACA CAGTTCTCCA CCTTGGACGG GGATGTGGAG 
GCCCCACCCT CCTACAGCAA GGCTGTGAGC TTCGAGCATC TGTCCTTCGG CTCGCAGGAC 
GACTCTGCAG GCAAGAACCG TATGGCAGTC AGCCCGGACG ACAGCCGCAC CGACAAGCTG 
GGGTCCAGCA TCTTACCTCC CCTGACCCAT GAGCTGACGG CCAGCGAGCT GCTGCTGAAA 
AAGATGTTTC ACGACGATGA GCTTGAAGAG TCAGAGAAAT TCTACGTGGG GCAGCCCCGA 
TTTCTGCTGC TCTTCTATGC CATGTACAAT ACCCTGGTGG CCCGCTCGGA GATGGTGTGC 
TACTTCGTGA TCATCCTCAA CCACATGGTC TCTGCCTCCA TGATCACGCT CCTGCTTCCC 
ATCCTCATCT TCCTCTGGGC CATGTTGTCC GTCCCCAGGC CCAGCCGCCG GTTCTGGATG 
ATGGCCATCG TCTATACTGA GGTGGCAATT GTAGTCAAGT ATTTCTTCCA ATTTGGGTTC 
TTTCCCTGGA ATAAGAATGT GGAGGTGAAC AAAGATAAAC CGTATCACCC CCCAAACATC 
ATAGGAGTGG AAAAGAAGGA AGGTTATGTT CTCTATGACC TCATCCAGCT CCTGGCTCTG 
TTCTTTCATC GATCAATTTT GAAGTGCCAT GGCTTATGGG ATGAAGATGA CATGACTGAA 
AGTGGCATGG CCAGGGAGGA ATCAGATGAT GAGCTCTCCC TCGGTCATGG CAGGAGGGAC 
TCCTCCGATT CTCTCAAGTC CATCAACCTG GCCGCGTCTG TGGAGTCAGT GCATGTGACC 
TTCCCGGAGC AGCAGACAGC TGTCCGGAGG AAGCGCTCCG GCAGCAGCTC CGAGCCATCC 
CAGAGATCCA GCTTTTCTTC AAACAGATCC CAAAGAGGCA GCACAAGCAC CCGAAACAGC 
AGTCAAAAAG GAAGCAGTGT TTTGAGTATT AAGCAAAAAG GCAAAAGGGA ACTTTATATG 
GAAAAGCTTC AAGAACATTT AATCAAAGCA AAAGCCTTTA CCATAAAGAA GACGCTGGAG 
ATCTATGTGC CCATCAAACA GTTCTTTTAC AACCTCATCC ACCCGGAGTA TAGCGCCGTG 
ACTGACGTGT ATGTACTCAT GTTCCTGGCT GACACTGTGG ACTTCATCAT CATTGTCTTC 
GGCTTTTGGG CCTTTGGGAA ACACTCAGCA GCTGCAGACA TCACCTCTTC ACTGTCAGAG 
GACCAGGTCC CGGGGCCGTT TTTGGTGATG GTCCTCATTC AGTTTGGAAC CATGGTGGTG 
GACCGAGCCC TCTACCTCAG GAAGACTGTA CTGGGAAAGG TCATCTTCCA GGTCATTCTT 
GTGTTCGGAA TTCACTTCTG GATGTTCTTC ATCTTACCTG GTGTGACTGA GAGGAAATTC 
AGCCAGAACC TGGTTGCCCA GCTTTGGTAC TTTGTGAAAT GTGTTTACTT CGGGTTGTCT 
GCTTACCAGA TCCGTTGTGG CTACCCAACG CGAGTCCTGG GGAACTTCCT CACCAAGAGC 
TACAATTACG TCAACCTCTT CTTATTCCAA GGGTTTCGCC TCGTGCCCTT TTTGACTGAG 
CTGAGGGCAG TGATGGACTG GGTGTGGACG GACACAACTT TGAGCCTGTC CAGCTGGATC 
TGTGTGGAGG ACATCTATGC TCACATATTC ATCCTGAAGT GTTGGCGGGA GTCGGAGAAG 
AGATACCCTC AGCCACGGGG CCAGAAGAAG AAGAAAGTGG TGAAGTATGG CATGGGAGGA 
ATGATCATCG TCCTGCTCAT CTGCATTGTC TGGTTTCCTC TTCTCTTCAT GTCTTTGATC 
AAATCTGTGG CTGGGGTCAT CAACCAGCCC CTGGACGTCT CCGTCACAAT TACCCTGGGA 
GGGTATCAGC CTATTTTCAC AATGAGTGCC CAACAAAGCC AGTTGAAAGT TATGGACCAG 
CAGAGCTTTA ACAAATTTAT ACAAGCTTTT TCTAGGGACA CCGGTGCTAT GCAATTTCTG 
GAAAATTATG AAAAAGAAGA CATAACAGTA GCAGAACTGG AAGGAAACTC AAATTCTTTG 
TGGACCATCA GCCCACCCAG TAAGCAGAAA ATGATACACG AACTCCTGGA CCCCAATAGT 
AGCTTCTCTG TTGTTTTTTC ATGGAGTATT CAGAGAAACT TAAGTCTGGG TGCAAAATCG 
GAAATAGCAA CAGATAAGCT TTCTTTTCCT CTTAAAAATA TTACTCGAAA GAATATCGCT 
AAAATGATAG CAGGCAACAG CACAGAAAGT TCAAAAACAC CAGTGACCAT AGAAAAGATT 
TATCCATATT ATGTGAAAGC ACCTAGTGAT TCTAACTCAA AACCTATAAA GCAACTTTTA 
TCTGAAAATA ATTTCATGGA TATTACCATC ATTTTGTCCA GAGACAATAC AACTAAATAT 
AACAGTGAGT GGTGGGTTCT CAACCTGACT GGAAACAGAA TATACAATCC GAACTCTCAG 
GCCCTGGAAC TGGTGGTCTT CAATGACAAA GTCAGTCCCC CAAGTCTGGG GTTCCTGGCT 
GGCTATGGTA TTATGGGATT ATATGCTTCA GTTGTCCTTG TGATTGGGAA ATTTGTCCGT 
GAATTCTTCA GTGGGATTTC TCACTCCATC ATGTTTGAAG AGCTTCCAAA TGTGGATCGA 
ATTTTGAAGT TGTGCACAGA TATTTTTTTA GTTCGAGAGA CAGGAGAACT GGAGCTAGAA 
GAAGATCTCT ATGCCAAATT AATATTCCTA TATCGCTCAC CAGAGACAAT GATCAAATGG 
ACTAGAGAAA AAACAAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIEZO2 ( XM_011525725.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524027.1 Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X4, mRNA.

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CloneID	OHu71133
Clone ID Related Accession (Same CDS sequence)	XM_011525725.3 , XM_011525725.2
Accession Version	XM_011525725.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	piezo-type mechanosensitive ion channel component 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525725.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG AAGTGGTGTG CGGGCTCATC TTCAGGCTGC TGCTGCCCAT CTGCCTGGCA 
GTAGCATGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC 
ATTCCTCTGT TCTCAGAACC AACAAAAACG ACGATGCAAG GACATACGGG ACGGTTATTA 
AAGTCTCTGT GCTTCATCAG TCTTTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG 
TTGGTGAGCC TTGAAGCTCA ACATCGTATT GCACCTGGCT ACAACTGCTC AACATGGGAA 
AAGACATTCC GGCAGATCGG CTTTGAAAGC TTAAAGGGAG CTGATGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCCTCTGT 
AGAAACATTG TTCAGAAACC TGTGACAGAC GAAGCAGCAC AGAGTAACCC GGAGTTTGAA 
AATGAAGAAT TGGCTGAAGG AGAAAAAATT GATTCAGAAG AGGCACTGAT CTATGAAGAG 
GATTTCAATG GAGGAGATGG TGTTGAAGGC GAGTTGGAAG AAAGCACGAA GTTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCCTCTAAG CTCAAGGAGT TCATTGGCAA CATGATCACC 
ACTGCTGGGA AAGTCGTTGT TACCATCTTA CTGGGCTCCT CGGGCATGAT GTTGCCGTCT 
TTGACATCAT CTGTGTATTT TTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACTGCT 
GGACATTTGA TTGGACTTTA TTTATACCAG TTCCAATTCT TTCAAGAGGC AGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAGTCAGTAA TTCAAACGGA CTGTTCAAGT 
ACTTGGAAGA TCATAGTGAA CCCGGACCTG TCGTGGTACC ACCACGCCAA CCCTATCCTC 
CTGCTGGTGA TGTACTACAC TCTGGCCACT CTGATCCGCA TCTGGCTGCA AGAGCCCCTT 
GTGCAGGATG AGGGGACCAA AGAAGAGGAC AAAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACAGCGGGGA GGAGGCGGAG CCTGTGGTAC GCAACCCATT ACCCCACTGA TGAGAGAAAA 
CTTTTATCCA TGACCCAGGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC 
GGCAACCCCG TGGATTACCA CACCATCCAC CCAAGCCTGC CCATGGAGAA CGGCCCTGGC 
AAAGCCGACC TCTACTCCAC CCCTCAGTAC CGGTGGGAGC CCTCTGATGA ATCCTCAGAA 
AAGCGAGAGG AGGAAGAGGA AGAGAAAGAA GAATTTGAAG AAGAAAGGAG CCGTGAGGAA 
AAAAGAAGTA TCAAAGTTCA TGCCATGGTC TCCGTATTCC AATTTATTAT GAAACAAAGT 
TACATCTGTG CCCTCATAGC TATGATGGCC TGGAGCATCA CCTATCACAG CTGGCTGACC 
TTCGTGCTGC TGATCTGGTC GTGCACTCTT TGGATGATTC GCAACAGAAG AAAATATGCC 
ATGATCAGCT CTCCCTTCAT GGTGGTTTAT GGAAACCTAT TGTTGATATT ACAGTATATA 
TGGAGTTTTG AACTTCCTGA AATTAAAAAA GTTCCAGGAT TTTTAGAAAA GAAAGAGCCA 
GGAGAACTTG CTTCCAAAAT CCTTTTCACC ATTACTTTTT GGCTACTGCT GAGGCAGCAC 
CTCACAGAGC AAAAAGCTCT GCAAGAAAAG GAAGCTCTTT TATCGGAAGT CAAAATTGGC 
AGTCAGGAAA ATGAAGAAAA AGATGAGGAA CTTCAAGATA TACAAGTGGA AGGAGAGCCC 
AAAGAGGAGG AAGAAGAGGA AGCGAAGGAA GAGAAGCAAG AGAGAAAGAA GGTAGAGCAA 
GAGGAAGCTG AAGAAGAAGA TGAGCAGGAC ATCATGAAAG TCCTGGGCAA TCTGGTGGTG 
GCCATGTTCA TCAAGTACTG GATCTACGTC TGCGGAGGCA TGTTCTTCTT CGTCAGCTTC 
GAGGGTAAAA TCGTAATGTA CAAAATCATC TACATGGTGC TGTTCCTGTT CTGTGTGGCC 
CTATACCAGG TGCACTATGA ATGGTGGAGG AAAATTCTAA AATATTTTTG GATGTCAGTG 
GTTATTTACA CTATGCTGGT GCTTATCTTT ATATACACAT ATCAGTTTGA GAACTTCCCA 
GGCCTGTGGC AAAATATGAC TGGACTGAAA AAAGAAAAGC TTGAGGATCT TGGCTTAAAG 
CAGTTTACTG TGGCTGAACT ATTCACTCGC ATATTCATCC CAACCTCCTT TCTGCTGGTG 
TGCATTTTAC ACCTGCACTA CTTCCATGAC CGGTTCCTTG AACTCACAGA CCTCAAGTCC 
ATTCCCAGCA AAGAAGACAA CACCATCTAC AGCCATGCCA AAGTCAATGG TCGCGTATAT 
CTAATAATAA ATAGACTGGC CCACCCGGAA GGAAGCCTCC CGGACCTCAC CATGATGCAT 
CTGACTGCCA GCCTGGAGAA GCCGGAGGTG AGGAAGTTGG CTGAGCCTGG GGAGGAGAAG 
CTTGAGGGCT ACTCTGAAAA AGCCCAGAAG GGTGATCTTG GGAAAGACAG CGAGGAGTCA 
GAGGAGGACG GAGAGGAAGA GGAGGAATCC GAGGAGGAGG AAGAAACATC AGACTTAAGG 
AACAAATGGC ACCTGGTGAT TGACCGCCTC ACTGTGCTCT TCTTAAAATT CCTGGAGTAT 
TTTCACAAGC TGCAGGTGTT CATGTGGTGG ATTTTGGAGT TGCACATCAT CAAAATCGTT 
TCCTCTTACA TTATCTGGGT TTCTGTGAAA GAGGTGTCTC TGTTCAACTA TGTATTTTTG 
ATTTCTTGGG CTTTTGCTCT GCCGTACGCC AAGCTGCGCC GTCTGGCTTC AAGTGTCTGC 
ACAGTCTGGA CGTGTGTGAT CATCGTCTGC AAAATGTTGT ACCAGCTCCA AACCATTAAG 
CCTGAGAACT TCTCTGTTAA CTGTTCCTTG CCAAATGAAA ATCAAACAAA CATCCCCTTT 
AATGAGTTGA ACAAGTCTCT GCTCTACAGC GCTCCTATCG ATCCTACAGA GTGGGTCGGC 
CTGCGGAAGT CTTCGCCTCT GCTAGTCTAC CTGAGGAATA ACCTCCTGAT GCTGGCTATC 
CTGGCCTTTG AAGTCACCAT TTACCGCCAT CAGGAATACT ATCGAGGTCG AAATAACCTG 
ACGGCCCCTG TGTCTAGAAC TATCTTTCAT GACATTACAA GACTACATCT AGATGATGGA 
CTTATTAATT GTGCCAAATA TTTCATTAAT TACTTCTTTT ACAAGTTTGG TCTGGAGACC 
TGTTTCCTAA TGTCAGTTAA CGTCATTGGC CAGCGAATGG ATTTCTATGC CATGATCCAC 
GCCTGCTGGC TGATCGCTGT CTTATATAGA CGCAGAAGGA AAGCCATCGC AGAGATCTGG 
CCCAAGTACT GCTGCTTCCT GGCATGCATC ATCACCTTCC AGTATTTCAT CTGCATTGGC 
ATCCCACCTG CTCCTTGCCG AGATTACCCG TGGAGATTCA AGGGTGCCAG CTTCAATGAC 
AACATCATAA AGTGGCTGTA CTTCCCAGAT TTCATTGTGC GGCCCAACCC TGTGTTTCTC 
GTCTATGACT TCATGCTGCT TCTGTGTGCC TCCTTACAAC GGCAGATTTT TGAGGATGAG 
AACAAGGCTG CAGTGCGAAT CATGGCAGGT GACAATGTCG AGATCTGCAT GAACCTTGAT 
GCGGCCTCCT TCAGCCAACA TAACCCTGTG CCAGATTTTA TTCACTGCAG ATCTTACTTA 
GACATGTCCA AAGTGATCAT CTTCAGCTAC CTCTTCTGGT TTGTGCTCAC CATCATCTTC 
ATCACTGGGA CCACCAGGAT CAGCATCTTT TGCATGGGGT ACCTGGTGGC CTGTTTCTAC 
TTCCTGCTCT TTGGGGGCGA TTTGCTGTTG AAACCCATCA AGAGCATCCT GCGCTACTGG 
GACTGGCTGA TCGCATACAA CGTTTTTGTG ATTACGATGA AAAATATCCT GTCAATAGGA 
GCATGTGGAT ACATTGGAAC ATTGGTGCAC AATAGTTGTT GGTTGATCCA GGCTTTCAGC 
CTGGCCTGCA CAGTCAAAGG CTATCAAATG CCTGCTGCTA ATTCACCCTG TACACTTCCC 
AGTGGGGAAG CAGGAATCAT TTGGGACAGC ATATGTTTTG CCTTCCTCCT GCTGCAAAGA 
AGAGTTTTCA TGAGTTATTA TTTTCTACAT GTTGTGGCTG ATATAAAAGC TTCCCAGATT 
CTGGCATCAA GAGGAGCTGA ACTTTTCCAG GCCACAATTG TAAAAGCTGT AAAGGCAAGA 
ATTGAGGAAG AGAAGAAGTC CATGGACCAG CTGAAGCGAC AGATGGATCG CATCAAGGCC 
AGGCAACAGA AATATAAAAA GGGTAAGGAG AGGATGCTGA GCTTGACCCA GGAGCCAGGG 
GAAGGCCAGG ACATGCAAAA ACTCTCTGAA GAGGATGATG AAAGAGAAGC AGACAAACAG 
AAAGCCAAGG GCAAAAAAAA GCAGTGGTGG CGGCCTTGGG TTGATCATGC TTCCATGGTC 
AGGAGTGGAG ATTATTATTT GTTTGAAACG GATAGTGAAG AGGAGGAAGA GGAAGAATTA 
AAGAAGGAAG ATGAAGAACC TCCACGAAGG TCAGCATTCC AGTTTGTTTA TCAAGCCTGG 
ATTACTGATC CTAAAACAGC ACTCCGACAA AGACACAAAG AGAAAAAAAG GTCTGCAAGA 
GAAGAACGGA AACGAAGGCG GAAAGGATCC AAGGAGGGTC CTGTGGAATG GGAAGACCGG 
GAGGATGAAC CAATCAAAAA GAAATCCGAT GGACCAGATA ATATCATCAA GAGGATATTT 
AATATTTTGA AATTTACCTG GGTCCTATTT CTGGCAACAG TGGACAGTTT CACTACTTGG 
CTTAACTCCA TTTCAAGGGA GCATATTGAT ATATCTACAG TTCTGAGAAT TGAACGATGC 
ATGCTGACCA GAGAAATTAA GAAGGGCAAT GTTCCAACTC GGGAGAGCAT CCACATGTAC 
TATCAGAACC ACATCATGAA CCTTTCCAGA GAGTCGGGAC TGGACACCAT TGACGAGCAT 
CCCGGAGCTG CTTCAGGTGC ACAGACAGCC CACAGGATGG ATAGTTTAGA TTCACATGAC 
AGTATCTCCA GCGAGCCCAC GCAGTGTACC ATGCTGTACT CACGCCAGGG GACCACTGAG 
ACCATCGAGG AGGTGGAGGC TGAGCAGGAG GAGGAGGCAG GGAGCACGGC GCCTGAGCCC 
AGGGAGGCCA AGGAGTACGA GGCCACTGGG TACGATGTGG GAGCCATGGG TGCCGAGGAG 
GCCAGCCTCA CCCCAGAGGA AGAGCTGACA CAGTTCTCCA CCTTGGACGG GGATGTGGAG 
GCCCCACCCT CCTACAGCAA GGCTGTGAGC TTCGAGCATC TGTCCTTCGG CTCGCAGGAC 
GACTCTGCAG GCAAGAACCG TATGGCAGTC AGCCCGGACG ACAGCCGCAC CGACAAGCTG 
GGGTCCAGCA TCTTACCTCC CCTGACCCAT GAGCTGACGG CCAGCGAGCT GCTGCTGAAA 
AAGATGTTTC ACGACGATGA GCTTGAAGAG TCAGAGAAAT TCTACGTGGG GCAGCCCCGA 
TTTCTGCTGC TCTTCTATGC CATGTACAAT ACCCTGGTGG CCCGCTCGGA GATGGTGTGC 
TACTTCGTGA TCATCCTCAA CCACATGGTC TCTGCCTCCA TGATCACGCT CCTGCTTCCC 
ATCCTCATCT TCCTCTGGGC CATGTTGTCC GTCCCCAGGC CCAGCCGCCG GTTCTGGATG 
ATGGCCATCG TCTATACTGA GGTGGCAATT GTAGTCAAGT ATTTCTTCCA ATTTGGGTTC 
TTTCCCTGGA ATAAGAATGT GGAGGTGAAC AAAGATAAAC CGTATCACCC CCCAAACATC 
ATAGGAGTGG AAAAGAAGGA AGGTTATGTT CTCTATGACC TCATCCAGCT CCTGGCTCTG 
TTCTTTCATC GATCAATTTT GAAGTGCCAT GGCTTATGGG ATGAAGATGA CATGACTGAA 
AGTGGCATGG CCAGGGAGGA ATCAGATGAT GAGCTCTCCC TCGGTCATGG CAGGAGGGAC 
TCCTCCGATT CTCTCAAGTC CATCAACCTG GCCGCGTCTG TGGAGTCAGT GCATGTGACC 
TTCCCGGAGC AGCAGACAGC TGTCCGGAGG AAGCGCTCCG GCAGCAGCTC CGAGCCATCC 
CAGAGATCCA GCTTTTCTTC AAACAGATCC CAAAGAGGCA GCACAAGCAC CCGAAACAGC 
AGTCAAAAAG GAAGCAGTGT TTTGAGTATT AAGCAAAAAG GCAAAAGGGA ACTTTATATG 
GAAAAGCTTC AAGAACATTT AATCAAAGCA AAAGCCTTTA CCATAAAGAA GACGCTGGAG 
ATCTATGTGC CCATCAAACA GTTCTTTTAC AACCTCATCC ACCCGGAGTA TAGCGCCGTG 
ACTGACGTGT ATGTACTCAT GTTCCTGGCT GACACTGTGG ACTTCATCAT CATTGTCTTC 
GGCTTTTGGG CCTTTGGGAA ACACTCAGCA GCTGCAGACA TCACCTCTTC ACTGTCAGAG 
GACCAGGTCC CGGGGCCGTT TTTGGTGATG GTCCTCATTC AGTTTGGAAC CATGGTGGTG 
GACCGAGCCC TCTACCTCAG GAAGACTGTA CTGGGAAAGG TCATCTTCCA GGTCATTCTT 
GTGTTCGGAA TTCACTTCTG GATGTTCTTC ATCTTACCTG GTGTGACTGA GAGGAAATTC 
AGCCAGAACC TGGTTGCCCA GCTTTGGTAC TTTGTGAAAT GTGTTTACTT CGGGTTGTCT 
GCTTACCAGA TCCGTTGTGG CTACCCAACG CGAGTCCTGG GGAACTTCCT CACCAAGAGC 
TACAATTACG TCAACCTCTT CTTATTCCAA GGGTTTCGCC TCGTGCCCTT TTTGACTGAG 
CTGAGGGCAG TGATGGACTG GGTGTGGACG GACACAACTT TGAGCCTGTC CAGCTGGATC 
TGTGTGGAGG ACATCTATGC TCACATATTC ATCCTGAAGT GTTGGCGGGA GTCGGAGAAG 
AGATACCCTC AGCCACGGGG CCAGAAGAAG AAGAAAGTGG TGAAGTATGG CATGGGAGGA 
ATGATCATCG TCCTGCTCAT CTGCATTGTC TGGTTTCCTC TTCTCTTCAT GTCTTTGATC 
AAATCTGTGG CTGGGGTCAT CAACCAGCCC CTGGACGTCT CCGTCACAAT TACCCTGGGA 
GGGTATCAGC CTATTTTCAC AATGAGTGCC CAACAAAGCC AGTTGAAAGT TATGGACCAG 
CAGAGCTTTA ACAAATTTAT ACAAGCTTTT TCTAGGGACA CCGGTGCTAT GCAATTTCTG 
GAAAATTATG AAAAAGAAGA CATAACAGTA GCAGAACTGG AAGGAAACTC AAATTCTTTG 
TGGACCATCA GCCCACCCAG TAAGCAGAAA ATGATACACG AACTCCTGGA CCCCAATAGT 
AGCTTCTCTG TTGTTTTTTC ATGGAGTATT CAGAGAAACT TAAGTCTGGG TGCAAAATCG 
GAAATAGCAA CAGATAAGCT TTCTTTTCCT CTTAAAAATA TTACTCGAAA GAATATCGCT 
AAAATGATAG CAGGCAACAG CACAGAAAGT TCAAAAACAC CAGTGACCAT AGAAAAGATT 
TATCCATATT ATGTGAAAGC ACCTAGTGAT TCTAACTCAA AACCTATAAA GCAACTTTTA 
TCTGAAAATA ATTTCATGGA TATTACCATC ATTTTGTCCA GAGACAATAC AACTAAATAT 
AACAGTGAGT GGTGGGTTCT CAACCTGACT GGAAACAGAA TATACAATCC GAACTCTCAG 
GCCCTGGAAC TGGTGGTCTT CAATGACAAA GTCAGTCCCC CAAGTCTGGG GTTCCTGGCT 
GGCTATGGTA TTATGGGATT ATATGCTTCA GTTGTCCTTG TGATTGGGAA ATTTGTCCGT 
GAATTCTTCA GTGGGATTTC TCACTCCATC ATGTTTGAAG AGCTTCCAAA TGTGGATCGA 
ATTTTGAAGT TGTGCACAGA TATTTTTTTA GTTCGAGAGA CAGGAGAACT GGAGCTAGAA 
GAAGATCTCT ATGCCAAATT AATATTCCTA TATCGCTCAC CAGAGACAAT GATCAAATGG 
ACTAGAGAAA AAACAAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524027.1
CDS	973..9273
Translation	MASEVVCGLIFRLLLPICLAVACAFRYNGLSFVYLIYLLLIPLFSEPTKTTMQGHTGRLLKSLCFISLSFLLLHIIFHITLVSLEAQHRIAPGYNCSTWEKTFRQIGFESLKGADAGNGIRVFVPDIGMFIASLTIWLLCRNIVQKPVTDEAAQSNPEFENEELAEGEKIDSEEALIYEEDFNGGDGVEGELEESTKLKMFRRLASVASKLKEFIGNMITTAGKVVVTILLGSSGMMLPSLTSSVYFFVFLGLCTWWSWCRTFDPLLFSCLCVLLAIFTAGHLIGLYLYQFQFFQEAVPPNDYYARLFGIKSVIQTDCSSTWKIIVNPDLSWYHHANPILLLVMYYTLATLIRIWLQEPLVQDEGTKEEDKALACSPIQITAGRRRSLWYATHYPTDERKLLSMTQDDYKPSDGLLVTVNGNPVDYHTIHPSLPMENGPGKADLYSTPQYRWEPSDESSEKREEEEEEKEEFEEERSREEKRSIKVHAMVSVFQFIMKQSYICALIAMMAWSITYHSWLTFVLLIWSCTLWMIRNRRKYAMISSPFMVVYGNLLLILQYIWSFELPEIKKVPGFLEKKEPGELASKILFTITFWLLLRQHLTEQKALQEKEALLSEVKIGSQENEEKDEELQDIQVEGEPKEEEEEEAKEEKQERKKVEQEEAEEEDEQDIMKVLGNLVVAMFIKYWIYVCGGMFFFVSFEGKIVMYKIIYMVLFLFCVALYQVHYEWWRKILKYFWMSVVIYTMLVLIFIYTYQFENFPGLWQNMTGLKKEKLEDLGLKQFTVAELFTRIFIPTSFLLVCILHLHYFHDRFLELTDLKSIPSKEDNTIYSHAKVNGRVYLIINRLAHPEGSLPDLTMMHLTASLEKPEVRKLAEPGEEKLEGYSEKAQKGDLGKDSEESEEDGEEEEESEEEEETSDLRNKWHLVIDRLTVLFLKFLEYFHKLQVFMWWILELHIIKIVSSYIIWVSVKEVSLFNYVFLISWAFALPYAKLRRLASSVCTVWTCVIIVCKMLYQLQTIKPENFSVNCSLPNENQTNIPFNELNKSLLYSAPIDPTEWVGLRKSSPLLVYLRNNLLMLAILAFEVTIYRHQEYYRGRNNLTAPVSRTIFHDITRLHLDDGLINCAKYFINYFFYKFGLETCFLMSVNVIGQRMDFYAMIHACWLIAVLYRRRRKAIAEIWPKYCCFLACIITFQYFICIGIPPAPCRDYPWRFKGASFNDNIIKWLYFPDFIVRPNPVFLVYDFMLLLCASLQRQIFEDENKAAVRIMAGDNVEICMNLDAASFSQHNPVPDFIHCRSYLDMSKVIIFSYLFWFVLTIIFITGTTRISIFCMGYLVACFYFLLFGGDLLLKPIKSILRYWDWLIAYNVFVITMKNILSIGACGYIGTLVHNSCWLIQAFSLACTVKGYQMPAANSPCTLPSGEAGIIWDSICFAFLLLQRRVFMSYYFLHVVADIKASQILASRGAELFQATIVKAVKARIEEEKKSMDQLKRQMDRIKARQQKYKKGKERMLSLTQEPGEGQDMQKLSEEDDEREADKQKAKGKKKQWWRPWVDHASMVRSGDYYLFETDSEEEEEEELKKEDEEPPRRSAFQFVYQAWITDPKTALRQRHKEKKRSAREERKRRRKGSKEGPVEWEDREDEPIKKKSDGPDNIIKRIFNILKFTWVLFLATVDSFTTWLNSISREHIDISTVLRIERCMLTREIKKGNVPTRESIHMYYQNHIMNLSRESGLDTIDEHPGAASGAQTAHRMDSLDSHDSISSEPTQCTMLYSRQGTTETIEEVEAEQEEEAGSTAPEPREAKEYEATGYDVGAMGAEEASLTPEEELTQFSTLDGDVEAPPSYSKAVSFEHLSFGSQDDSAGKNRMAVSPDDSRTDKLGSSILPPLTHELTASELLLKKMFHDDELEESEKFYVGQPRFLLLFYAMYNTLVARSEMVCYFVIILNHMVSASMITLLLPILIFLWAMLSVPRPSRRFWMMAIVYTEVAIVVKYFFQFGFFPWNKNVEVNKDKPYHPPNIIGVEKKEGYVLYDLIQLLALFFHRSILKCHGLWDEDDMTESGMAREESDDELSLGHGRRDSSDSLKSINLAASVESVHVTFPEQQTAVRRKRSGSSSEPSQRSSFSSNRSQRGSTSTRNSSQKGSSVLSIKQKGKRELYMEKLQEHLIKAKAFTIKKTLEIYVPIKQFFYNLIHPEYSAVTDVYVLMFLADTVDFIIIVFGFWAFGKHSAAADITSSLSEDQVPGPFLVMVLIQFGTMVVDRALYLRKTVLGKVIFQVILVFGIHFWMFFILPGVTERKFSQNLVAQLWYFVKCVYFGLSAYQIRCGYPTRVLGNFLTKSYNYVNLFLFQGFRLVPFLTELRAVMDWVWTDTTLSLSSWICVEDIYAHIFILKCWRESEKRYPQPRGQKKKKVVKYGMGGMIIVLLICIVWFPLLFMSLIKSVAGVINQPLDVSVTITLGGYQPIFTMSAQQSQLKVMDQQSFNKFIQAFSRDTGAMQFLENYEKEDITVAELEGNSNSLWTISPPSKQKMIHELLDPNSSFSVVFSWSIQRNLSLGAKSEIATDKLSFPLKNITRKNIAKMIAGNSTESSKTPVTIEKIYPYYVKAPSDSNSKPIKQLLSENNFMDITIILSRDNTTKYNSEWWVLNLTGNRIYNPNSQALELVVFNDKVSPPSLGFLAGYGIMGLYASVVLVIGKFVREFFSGISHSIMFEELPNVDRILKLCTDIFLVRETGELELEEDLYAKLIFLYRSPETMIKWTREKTN

Target ORF information:

RefSeq Version	XM_011525725.2
Organism	Homo sapiens(human)
Definition	Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525725.2

ORF Insert Sequence:

ATGGCCTCAG AAGTGGTGTG CGGGCTCATC TTCAGGCTGC TGCTGCCCAT CTGCCTGGCA 
GTAGCATGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC 
ATTCCTCTGT TCTCAGAACC AACAAAAACG ACGATGCAAG GACATACGGG ACGGTTATTA 
AAGTCTCTGT GCTTCATCAG TCTTTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG 
TTGGTGAGCC TTGAAGCTCA ACATCGTATT GCACCTGGCT ACAACTGCTC AACATGGGAA 
AAGACATTCC GGCAGATCGG CTTTGAAAGC TTAAAGGGAG CTGATGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCCTCTGT 
AGAAACATTG TTCAGAAACC TGTGACAGAC GAAGCAGCAC AGAGTAACCC GGAGTTTGAA 
AATGAAGAAT TGGCTGAAGG AGAAAAAATT GATTCAGAAG AGGCACTGAT CTATGAAGAG 
GATTTCAATG GAGGAGATGG TGTTGAAGGC GAGTTGGAAG AAAGCACGAA GTTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCCTCTAAG CTCAAGGAGT TCATTGGCAA CATGATCACC 
ACTGCTGGGA AAGTCGTTGT TACCATCTTA CTGGGCTCCT CGGGCATGAT GTTGCCGTCT 
TTGACATCAT CTGTGTATTT TTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACTGCT 
GGACATTTGA TTGGACTTTA TTTATACCAG TTCCAATTCT TTCAAGAGGC AGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAGTCAGTAA TTCAAACGGA CTGTTCAAGT 
ACTTGGAAGA TCATAGTGAA CCCGGACCTG TCGTGGTACC ACCACGCCAA CCCTATCCTC 
CTGCTGGTGA TGTACTACAC TCTGGCCACT CTGATCCGCA TCTGGCTGCA AGAGCCCCTT 
GTGCAGGATG AGGGGACCAA AGAAGAGGAC AAAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACAGCGGGGA GGAGGCGGAG CCTGTGGTAC GCAACCCATT ACCCCACTGA TGAGAGAAAA 
CTTTTATCCA TGACCCAGGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC 
GGCAACCCCG TGGATTACCA CACCATCCAC CCAAGCCTGC CCATGGAGAA CGGCCCTGGC 
AAAGCCGACC TCTACTCCAC CCCTCAGTAC CGGTGGGAGC CCTCTGATGA ATCCTCAGAA 
AAGCGAGAGG AGGAAGAGGA AGAGAAAGAA GAATTTGAAG AAGAAAGGAG CCGTGAGGAA 
AAAAGAAGTA TCAAAGTTCA TGCCATGGTC TCCGTATTCC AATTTATTAT GAAACAAAGT 
TACATCTGTG CCCTCATAGC TATGATGGCC TGGAGCATCA CCTATCACAG CTGGCTGACC 
TTCGTGCTGC TGATCTGGTC GTGCACTCTT TGGATGATTC GCAACAGAAG AAAATATGCC 
ATGATCAGCT CTCCCTTCAT GGTGGTTTAT GGAAACCTAT TGTTGATATT ACAGTATATA 
TGGAGTTTTG AACTTCCTGA AATTAAAAAA GTTCCAGGAT TTTTAGAAAA GAAAGAGCCA 
GGAGAACTTG CTTCCAAAAT CCTTTTCACC ATTACTTTTT GGCTACTGCT GAGGCAGCAC 
CTCACAGAGC AAAAAGCTCT GCAAGAAAAG GAAGCTCTTT TATCGGAAGT CAAAATTGGC 
AGTCAGGAAA ATGAAGAAAA AGATGAGGAA CTTCAAGATA TACAAGTGGA AGGAGAGCCC 
AAAGAGGAGG AAGAAGAGGA AGCGAAGGAA GAGAAGCAAG AGAGAAAGAA GGTAGAGCAA 
GAGGAAGCTG AAGAAGAAGA TGAGCAGGAC ATCATGAAAG TCCTGGGCAA TCTGGTGGTG 
GCCATGTTCA TCAAGTACTG GATCTACGTC TGCGGAGGCA TGTTCTTCTT CGTCAGCTTC 
GAGGGTAAAA TCGTAATGTA CAAAATCATC TACATGGTGC TGTTCCTGTT CTGTGTGGCC 
CTATACCAGG TGCACTATGA ATGGTGGAGG AAAATTCTAA AATATTTTTG GATGTCAGTG 
GTTATTTACA CTATGCTGGT GCTTATCTTT ATATACACAT ATCAGTTTGA GAACTTCCCA 
GGCCTGTGGC AAAATATGAC TGGACTGAAA AAAGAAAAGC TTGAGGATCT TGGCTTAAAG 
CAGTTTACTG TGGCTGAACT ATTCACTCGC ATATTCATCC CAACCTCCTT TCTGCTGGTG 
TGCATTTTAC ACCTGCACTA CTTCCATGAC CGGTTCCTTG AACTCACAGA CCTCAAGTCC 
ATTCCCAGCA AAGAAGACAA CACCATCTAC AGCCATGCCA AAGTCAATGG TCGCGTATAT 
CTAATAATAA ATAGACTGGC CCACCCGGAA GGAAGCCTCC CGGACCTCAC CATGATGCAT 
CTGACTGCCA GCCTGGAGAA GCCGGAGGTG AGGAAGTTGG CTGAGCCTGG GGAGGAGAAG 
CTTGAGGGCT ACTCTGAAAA AGCCCAGAAG GGTGATCTTG GGAAAGACAG CGAGGAGTCA 
GAGGAGGACG GAGAGGAAGA GGAGGAATCC GAGGAGGAGG AAGAAACATC AGACTTAAGG 
AACAAATGGC ACCTGGTGAT TGACCGCCTC ACTGTGCTCT TCTTAAAATT CCTGGAGTAT 
TTTCACAAGC TGCAGGTGTT CATGTGGTGG ATTTTGGAGT TGCACATCAT CAAAATCGTT 
TCCTCTTACA TTATCTGGGT TTCTGTGAAA GAGGTGTCTC TGTTCAACTA TGTATTTTTG 
ATTTCTTGGG CTTTTGCTCT GCCGTACGCC AAGCTGCGCC GTCTGGCTTC AAGTGTCTGC 
ACAGTCTGGA CGTGTGTGAT CATCGTCTGC AAAATGTTGT ACCAGCTCCA AACCATTAAG 
CCTGAGAACT TCTCTGTTAA CTGTTCCTTG CCAAATGAAA ATCAAACAAA CATCCCCTTT 
AATGAGTTGA ACAAGTCTCT GCTCTACAGC GCTCCTATCG ATCCTACAGA GTGGGTCGGC 
CTGCGGAAGT CTTCGCCTCT GCTAGTCTAC CTGAGGAATA ACCTCCTGAT GCTGGCTATC 
CTGGCCTTTG AAGTCACCAT TTACCGCCAT CAGGAATACT ATCGAGGTCG AAATAACCTG 
ACGGCCCCTG TGTCTAGAAC TATCTTTCAT GACATTACAA GACTACATCT AGATGATGGA 
CTTATTAATT GTGCCAAATA TTTCATTAAT TACTTCTTTT ACAAGTTTGG TCTGGAGACC 
TGTTTCCTAA TGTCAGTTAA CGTCATTGGC CAGCGAATGG ATTTCTATGC CATGATCCAC 
GCCTGCTGGC TGATCGCTGT CTTATATAGA CGCAGAAGGA AAGCCATCGC AGAGATCTGG 
CCCAAGTACT GCTGCTTCCT GGCATGCATC ATCACCTTCC AGTATTTCAT CTGCATTGGC 
ATCCCACCTG CTCCTTGCCG AGATTACCCG TGGAGATTCA AGGGTGCCAG CTTCAATGAC 
AACATCATAA AGTGGCTGTA CTTCCCAGAT TTCATTGTGC GGCCCAACCC TGTGTTTCTC 
GTCTATGACT TCATGCTGCT TCTGTGTGCC TCCTTACAAC GGCAGATTTT TGAGGATGAG 
AACAAGGCTG CAGTGCGAAT CATGGCAGGT GACAATGTCG AGATCTGCAT GAACCTTGAT 
GCGGCCTCCT TCAGCCAACA TAACCCTGTG CCAGATTTTA TTCACTGCAG ATCTTACTTA 
GACATGTCCA AAGTGATCAT CTTCAGCTAC CTCTTCTGGT TTGTGCTCAC CATCATCTTC 
ATCACTGGGA CCACCAGGAT CAGCATCTTT TGCATGGGGT ACCTGGTGGC CTGTTTCTAC 
TTCCTGCTCT TTGGGGGCGA TTTGCTGTTG AAACCCATCA AGAGCATCCT GCGCTACTGG 
GACTGGCTGA TCGCATACAA CGTTTTTGTG ATTACGATGA AAAATATCCT GTCAATAGGA 
GCATGTGGAT ACATTGGAAC ATTGGTGCAC AATAGTTGTT GGTTGATCCA GGCTTTCAGC 
CTGGCCTGCA CAGTCAAAGG CTATCAAATG CCTGCTGCTA ATTCACCCTG TACACTTCCC 
AGTGGGGAAG CAGGAATCAT TTGGGACAGC ATATGTTTTG CCTTCCTCCT GCTGCAAAGA 
AGAGTTTTCA TGAGTTATTA TTTTCTACAT GTTGTGGCTG ATATAAAAGC TTCCCAGATT 
CTGGCATCAA GAGGAGCTGA ACTTTTCCAG GCCACAATTG TAAAAGCTGT AAAGGCAAGA 
ATTGAGGAAG AGAAGAAGTC CATGGACCAG CTGAAGCGAC AGATGGATCG CATCAAGGCC 
AGGCAACAGA AATATAAAAA GGGTAAGGAG AGGATGCTGA GCTTGACCCA GGAGCCAGGG 
GAAGGCCAGG ACATGCAAAA ACTCTCTGAA GAGGATGATG AAAGAGAAGC AGACAAACAG 
AAAGCCAAGG GCAAAAAAAA GCAGTGGTGG CGGCCTTGGG TTGATCATGC TTCCATGGTC 
AGGAGTGGAG ATTATTATTT GTTTGAAACG GATAGTGAAG AGGAGGAAGA GGAAGAATTA 
AAGAAGGAAG ATGAAGAACC TCCACGAAGG TCAGCATTCC AGTTTGTTTA TCAAGCCTGG 
ATTACTGATC CTAAAACAGC ACTCCGACAA AGACACAAAG AGAAAAAAAG GTCTGCAAGA 
GAAGAACGGA AACGAAGGCG GAAAGGATCC AAGGAGGGTC CTGTGGAATG GGAAGACCGG 
GAGGATGAAC CAATCAAAAA GAAATCCGAT GGACCAGATA ATATCATCAA GAGGATATTT 
AATATTTTGA AATTTACCTG GGTCCTATTT CTGGCAACAG TGGACAGTTT CACTACTTGG 
CTTAACTCCA TTTCAAGGGA GCATATTGAT ATATCTACAG TTCTGAGAAT TGAACGATGC 
ATGCTGACCA GAGAAATTAA GAAGGGCAAT GTTCCAACTC GGGAGAGCAT CCACATGTAC 
TATCAGAACC ACATCATGAA CCTTTCCAGA GAGTCGGGAC TGGACACCAT TGACGAGCAT 
CCCGGAGCTG CTTCAGGTGC ACAGACAGCC CACAGGATGG ATAGTTTAGA TTCACATGAC 
AGTATCTCCA GCGAGCCCAC GCAGTGTACC ATGCTGTACT CACGCCAGGG GACCACTGAG 
ACCATCGAGG AGGTGGAGGC TGAGCAGGAG GAGGAGGCAG GGAGCACGGC GCCTGAGCCC 
AGGGAGGCCA AGGAGTACGA GGCCACTGGG TACGATGTGG GAGCCATGGG TGCCGAGGAG 
GCCAGCCTCA CCCCAGAGGA AGAGCTGACA CAGTTCTCCA CCTTGGACGG GGATGTGGAG 
GCCCCACCCT CCTACAGCAA GGCTGTGAGC TTCGAGCATC TGTCCTTCGG CTCGCAGGAC 
GACTCTGCAG GCAAGAACCG TATGGCAGTC AGCCCGGACG ACAGCCGCAC CGACAAGCTG 
GGGTCCAGCA TCTTACCTCC CCTGACCCAT GAGCTGACGG CCAGCGAGCT GCTGCTGAAA 
AAGATGTTTC ACGACGATGA GCTTGAAGAG TCAGAGAAAT TCTACGTGGG GCAGCCCCGA 
TTTCTGCTGC TCTTCTATGC CATGTACAAT ACCCTGGTGG CCCGCTCGGA GATGGTGTGC 
TACTTCGTGA TCATCCTCAA CCACATGGTC TCTGCCTCCA TGATCACGCT CCTGCTTCCC 
ATCCTCATCT TCCTCTGGGC CATGTTGTCC GTCCCCAGGC CCAGCCGCCG GTTCTGGATG 
ATGGCCATCG TCTATACTGA GGTGGCAATT GTAGTCAAGT ATTTCTTCCA ATTTGGGTTC 
TTTCCCTGGA ATAAGAATGT GGAGGTGAAC AAAGATAAAC CGTATCACCC CCCAAACATC 
ATAGGAGTGG AAAAGAAGGA AGGTTATGTT CTCTATGACC TCATCCAGCT CCTGGCTCTG 
TTCTTTCATC GATCAATTTT GAAGTGCCAT GGCTTATGGG ATGAAGATGA CATGACTGAA 
AGTGGCATGG CCAGGGAGGA ATCAGATGAT GAGCTCTCCC TCGGTCATGG CAGGAGGGAC 
TCCTCCGATT CTCTCAAGTC CATCAACCTG GCCGCGTCTG TGGAGTCAGT GCATGTGACC 
TTCCCGGAGC AGCAGACAGC TGTCCGGAGG AAGCGCTCCG GCAGCAGCTC CGAGCCATCC 
CAGAGATCCA GCTTTTCTTC AAACAGATCC CAAAGAGGCA GCACAAGCAC CCGAAACAGC 
AGTCAAAAAG GAAGCAGTGT TTTGAGTATT AAGCAAAAAG GCAAAAGGGA ACTTTATATG 
GAAAAGCTTC AAGAACATTT AATCAAAGCA AAAGCCTTTA CCATAAAGAA GACGCTGGAG 
ATCTATGTGC CCATCAAACA GTTCTTTTAC AACCTCATCC ACCCGGAGTA TAGCGCCGTG 
ACTGACGTGT ATGTACTCAT GTTCCTGGCT GACACTGTGG ACTTCATCAT CATTGTCTTC 
GGCTTTTGGG CCTTTGGGAA ACACTCAGCA GCTGCAGACA TCACCTCTTC ACTGTCAGAG 
GACCAGGTCC CGGGGCCGTT TTTGGTGATG GTCCTCATTC AGTTTGGAAC CATGGTGGTG 
GACCGAGCCC TCTACCTCAG GAAGACTGTA CTGGGAAAGG TCATCTTCCA GGTCATTCTT 
GTGTTCGGAA TTCACTTCTG GATGTTCTTC ATCTTACCTG GTGTGACTGA GAGGAAATTC 
AGCCAGAACC TGGTTGCCCA GCTTTGGTAC TTTGTGAAAT GTGTTTACTT CGGGTTGTCT 
GCTTACCAGA TCCGTTGTGG CTACCCAACG CGAGTCCTGG GGAACTTCCT CACCAAGAGC 
TACAATTACG TCAACCTCTT CTTATTCCAA GGGTTTCGCC TCGTGCCCTT TTTGACTGAG 
CTGAGGGCAG TGATGGACTG GGTGTGGACG GACACAACTT TGAGCCTGTC CAGCTGGATC 
TGTGTGGAGG ACATCTATGC TCACATATTC ATCCTGAAGT GTTGGCGGGA GTCGGAGAAG 
AGATACCCTC AGCCACGGGG CCAGAAGAAG AAGAAAGTGG TGAAGTATGG CATGGGAGGA 
ATGATCATCG TCCTGCTCAT CTGCATTGTC TGGTTTCCTC TTCTCTTCAT GTCTTTGATC 
AAATCTGTGG CTGGGGTCAT CAACCAGCCC CTGGACGTCT CCGTCACAAT TACCCTGGGA 
GGGTATCAGC CTATTTTCAC AATGAGTGCC CAACAAAGCC AGTTGAAAGT TATGGACCAG 
CAGAGCTTTA ACAAATTTAT ACAAGCTTTT TCTAGGGACA CCGGTGCTAT GCAATTTCTG 
GAAAATTATG AAAAAGAAGA CATAACAGTA GCAGAACTGG AAGGAAACTC AAATTCTTTG 
TGGACCATCA GCCCACCCAG TAAGCAGAAA ATGATACACG AACTCCTGGA CCCCAATAGT 
AGCTTCTCTG TTGTTTTTTC ATGGAGTATT CAGAGAAACT TAAGTCTGGG TGCAAAATCG 
GAAATAGCAA CAGATAAGCT TTCTTTTCCT CTTAAAAATA TTACTCGAAA GAATATCGCT 
AAAATGATAG CAGGCAACAG CACAGAAAGT TCAAAAACAC CAGTGACCAT AGAAAAGATT 
TATCCATATT ATGTGAAAGC ACCTAGTGAT TCTAACTCAA AACCTATAAA GCAACTTTTA 
TCTGAAAATA ATTTCATGGA TATTACCATC ATTTTGTCCA GAGACAATAC AACTAAATAT 
AACAGTGAGT GGTGGGTTCT CAACCTGACT GGAAACAGAA TATACAATCC GAACTCTCAG 
GCCCTGGAAC TGGTGGTCTT CAATGACAAA GTCAGTCCCC CAAGTCTGGG GTTCCTGGCT 
GGCTATGGTA TTATGGGATT ATATGCTTCA GTTGTCCTTG TGATTGGGAA ATTTGTCCGT 
GAATTCTTCA GTGGGATTTC TCACTCCATC ATGTTTGAAG AGCTTCCAAA TGTGGATCGA 
ATTTTGAAGT TGTGCACAGA TATTTTTTTA GTTCGAGAGA CAGGAGAACT GGAGCTAGAA 
GAAGATCTCT ATGCCAAATT AATATTCCTA TATCGCTCAC CAGAGACAAT GATCAAATGG 
ACTAGAGAAA AAACAAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIEZO2 ( XM_011525726.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524028.1 Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X5, mRNA.

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CloneID	OHu71134
Clone ID Related Accession (Same CDS sequence)	XM_011525726.3 , XM_011525726.2
Accession Version	XM_011525726.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	piezo-type mechanosensitive ion channel component 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011525726.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG AAGTGGTGTG CGGGCTCATC TTCAGGCTGC TGCTGCCCAT CTGCCTGGCA 
GTAGCATGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC 
ATTCCTCTGT TCTCAGAACC AACAAAAACG ACGATGCAAG GACATACGGG ACGGTTATTA 
AAGTCTCTGT GCTTCATCAG TCTTTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG 
TTGGTGAGCC TTGAAGCTCA ACATCGTATT GCACCTGGCT ACAACTGCTC AACATGGGAA 
AAGACATTCC GGCAGATCGG CTTTGAAAGC TTAAAGGGAG CTGATGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCCTCTGT 
AGAAACATTG TTCAGAAACC TGTGACAGAC GAAGCAGCAC AGAGTAACCC GGAGTTTGAA 
AATGAAGAAT TGGCTGAAGG AGAAAAAATT GATTCAGAAG AGGCACTGAT CTATGAAGAG 
GATTTCAATG GAGGAGATGG TGTTGAAGGC GAGTTGGAAG AAAGCACGAA GTTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCCTCTAAG CTCAAGGAGT TCATTGGCAA CATGATCACC 
ACTGCTGGGA AAGTCGTTGT TACCATCTTA CTGGGCTCCT CGGGCATGAT GTTGCCGTCT 
TTGACATCAT CTGTGTATTT TTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACTGCT 
GGACATTTGA TTGGACTTTA TTTATACCAG TTCCAATTCT TTCAAGAGGC AGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAGTCAGTAA TTCAAACGGA CTGTTCAAGT 
ACTTGGAAGA TCATAGTGAA CCCGGACCTG TCGTGGTACC ACCACGCCAA CCCTATCCTC 
CTGCTGGTGA TGTACTACAC TCTGGCCACT CTGATCCGCA TCTGGCTGCA AGAGCCCCTT 
GTGCAGGATG AGGGGACCAA AGAAGAGGAC AAAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACAGCGGGGA GGAGGCGGAG CCTGTGGTAC GCAACCCATT ACCCCACTGA TGAGAGAAAA 
CTTTTATCCA TGACCCAGGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC 
GGCAACCCCG TGGATTACCA CACCATCCAC CCAAGCCTGC CCATGGAGAA CGGCCCTGGC 
AAAGCCGACC TCTACTCCAC CCCTCAGTAC CGGTGGGAGC CCTCTGATGA ATCCTCAGAA 
AAGCGAGAGG AGGAAGAGGA AGAGAAAGAA GAATTTGAAG AAGAAAGGAG CCGTGAGGAA 
AAAAGAAGTA TCAAAGTTCA TGCCATGGTC TCCGTATTCC AATTTATTAT GAAACAAAGT 
TACATCTGTG CCCTCATAGC TATGATGGCC TGGAGCATCA CCTATCACAG CTGGCTGACC 
TTCGTGCTGC TGATCTGGTC GTGCACTCTT TGGATGATTC GCAACAGAAG AAAATATGCC 
ATGATCAGCT CTCCCTTCAT GGTGGTTTAT GGAAACCTAT TGTTGATATT ACAGTATATA 
TGGAGTTTTG AACTTCCTGA AATTAAAAAA GTTCCAGGAT TTTTAGAAAA GAAAGAGCCA 
GGAGAACTTG CTTCCAAAAT CCTTTTCACC ATTACTTTTT GGCTACTGCT GAGGCAGCAC 
CTCACAGAGC AAAAAGCTCT GCAAGAAAAG GAAGCTCTTT TATCGGAAGT CAAAATTGGC 
AGTCAGGAAA ATGAAGAAAA AGATGAGGAA CTTCAAGATA TACAAGTGGA AGGAGAGCCC 
AAAGAGGAGG AAGAAGAGGA AGCGAAGGAA GAGAAGCAAG AGAGAAAGAA GGTAGAGCAA 
GAGGAAGCTG AAGAAGAAGA TGAGCAGGAC ATCATGAAAG TCCTGGGCAA TCTGGTGGTG 
GCCATGTTCA TCAAGTACTG GATCTACGTC TGCGGAGGCA TGTTCTTCTT CGTCAGCTTC 
GAGGGTAAAA TCGTAATGTA CAAAATCATC TACATGGTGC TGTTCCTGTT CTGTGTGGCC 
CTATACCAGG TGCACTATGA ATGGTGGAGG AAAATTCTAA AATATTTTTG GATGTCAGTG 
GTTATTTACA CTATGCTGGT GCTTATCTTT ATATACACAT ATCAGTTTGA GAACTTCCCA 
GGCCTGTGGC AAAATATGAC TGGACTGAAA AAAGAAAAGC TTGAGGATCT TGGCTTAAAG 
CAGTTTACTG TGGCTGAACT ATTCACTCGC ATATTCATCC CAACCTCCTT TCTGCTGGTG 
TGCATTTTAC ACCTGCACTA CTTCCATGAC CGGTTCCTTG AACTCACAGA CCTCAAGTCC 
ATTCCCAGCA AAGAAGACAA CACCATCTAC AGCCATGCCA AAGTCAATGG TCGCGTATAT 
CTAATAATAA ATAGGATCTC ACACAGTCTT CCCACGGTTG CCAATCGACA GTTCGCATCA 
GTAACTCTCA GCATCAAGAA AAAATTACCA ATCCACCAAA ATGAACTGGC CCACCCGGAA 
GGAAGCCTCC CGGACCTCAC CATGATGCAT CTGACTGCCA GCCTGGAGAA GCCGGAGGTG 
AGGAAGTTGG CTGAGCCTGG GGAGGAGAAG CTTGAGGGCT ACTCTGAAAA AGCCCAGAAG 
GGTGATCTTG GGAAAGACAG CGAGGAGTCA GAGGAGGACG GAGAGGAAGA GGAGGAATCC 
GAGGAGGAGG AAGAAACATC AGACTTAAGG AACAAATGGC ACCTGGTGAT TGACCGCCTC 
ACTGTGCTCT TCTTAAAATT CCTGGAGTAT TTTCACAAGC TGCAGGTGTT CATGTGGTGG 
ATTTTGGAGT TGCACATCAT CAAAATCGTT TCCTCTTACA TTATCTGGGT TTCTGTGAAA 
GAGGTGTCTC TGTTCAACTA TGTATTTTTG ATTTCTTGGG CTTTTGCTCT GCCGTACGCC 
AAGCTGCGCC GTCTGGCTTC AAGTGTCTGC ACAGTCTGGA CGTGTGTGAT CATCGTCTGC 
AAAATGTTGT ACCAGCTCCA AACCATTAAG CCTGAGAACT TCTCTGTTAA CTGTTCCTTG 
CCAAATGAAA ATCAAACAAA CATCCCCTTT AATGAGTTGA ACAAGTCTCT GCTCTACAGC 
GCTCCTATCG ATCCTACAGA GTGGGTCGGC CTGCGGAAGT CTTCGCCTCT GCTAGTCTAC 
CTGAGGAATA ACCTCCTGAT GCTGGCTATC CTGGCCTTTG AAGTCACCAT TTACCGCCAT 
CAGGAATACT ATCGAGGTCG AAATAACCTG ACGGCCCCTG TGTCTAGAAC TATCTTTCAT 
GACATTACAA GACTACATCT AGATGATGGA CTTATTAATT GTGCCAAATA TTTCATTAAT 
TACTTCTTTT ACAAGTTTGG TCTGGAGACC TGTTTCCTAA TGTCAGTTAA CGTCATTGGC 
CAGCGAATGG ATTTCTATGC CATGATCCAC GCCTGCTGGC TGATCGCTGT CTTATATAGA 
CGCAGAAGGA AAGCCATCGC AGAGATCTGG CCCAAGTACT GCTGCTTCCT GGCATGCATC 
ATCACCTTCC AGTATTTCAT CTGCATTGGC ATCCCACCTG CTCCTTGCCG AGATTACCCG 
TGGAGATTCA AGGGTGCCAG CTTCAATGAC AACATCATAA AGTGGCTGTA CTTCCCAGAT 
TTCATTGTGC GGCCCAACCC TGTGTTTCTC GTCTATGACT TCATGCTGCT TCTGTGTGCC 
TCCTTACAAC GGCAGATTTT TGAGGATGAG AACAAGGCTG CAGTGCGAAT CATGGCAGGT 
GACAATGTCG AGATCTGCAT GAACCTTGAT GCGGCCTCCT TCAGCCAACA TAACCCTGTG 
CCAGATTTTA TTCACTGCAG ATCTTACTTA GACATGTCCA AAGTGATCAT CTTCAGCTAC 
CTCTTCTGGT TTGTGCTCAC CATCATCTTC ATCACTGGGA CCACCAGGAT CAGCATCTTT 
TGCATGGGGT ACCTGGTGGC CTGTTTCTAC TTCCTGCTCT TTGGGGGCGA TTTGCTGTTG 
AAACCCATCA AGAGCATCCT GCGCTACTGG GACTGGCTGA TCGCATACAA CGTTTTTGTG 
ATTACGATGA AAAATATCCT GTCAATAGGA GCATGTGGAT ACATTGGAAC ATTGGTGCAC 
AATAGTTGTT GGTTGATCCA GGCTTTCAGC CTGGCCTGCA CAGTCAAAGG CTATCAAATG 
CCTGCTGCTA ATTCACCCTG TACACTTCCC AGTGGGGAAG CAGGAATCAT TTGGGACAGC 
ATATGTTTTG CCTTCCTCCT GCTGCAAAGA AGAGTTTTCA TGAGTTATTA TTTTCTACAT 
GTTGTGGCTG ATATAAAAGC TTCCCAGATT CTGGCATCAA GAGGAGCTGA ACTTTTCCAG 
GCCACAATTG TAAAAGCTGT AAAGGCAAGA ATTGAGGAAG AGAAGAAGTC CATGGACCAG 
CTGAAGCGAC AGATGGATCG CATCAAGGCC AGGCAACAGA AATATAAAAA GGGTAAGGAG 
AGGATGCTGA GCTTGACCCA GGAGCCAGGG GAAGGCCAGG ACATGCAAAA ACTCTCTGAA 
GAGGATGATG AAAGAGAAGC AGACAAACAG AAAGCCAAGG GCAAAAAAAA GCAGTGGTGG 
CGGCCTTGGG TTGATCATGC TTCCATGGTC AGGAGTGGAG ATTATTATTT GTTTGAAACG 
GATAGTGAAG AGGAGGAAGA GGAAGAATTA AAGAAGGAAG ATGAAGAACC TCCACGAAGG 
TCAGCATTCC AGTTTGTTTA TCAAGCCTGG ATTACTGATC CTAAAACAGC ACTCCGACAA 
AGACACAAAG AGAAAAAAAG GTCTGCAAGA GAAGAACGGA AACGAAGGCG GAAAGGATCC 
AAGGAGGGTC CTGTGGAATG GGAAGACCGG GAGGATGAAC CAATCAAAAA GAAATCCGAT 
GGACCAGATA ATATCATCAA GAGGATATTT AATATTTTGA AATTTACCTG GGTCCTATTT 
CTGGCAACAG TGGACAGTTT CACTACTTGG CTTAACTCCA TTTCAAGGGA GCATATTGAT 
ATATCTACAG TTCTGAGAAT TGAACGATGC ATGCTGACCA GAGAAATTAA GAAGGGCAAT 
GTTCCAACTC GGGAGAGCAT CCACATGTAC TATCAGAACC ACATCATGAA CCTTTCCAGA 
GAGTCGGGAC TGGACACCAT TGACGAGCAT CCCGGAGCTG CTTCAGGTGC ACAGACAGCC 
CACAGGATGG ATAGTTTAGA TTCACATGAC AGTATCTCCA GCGAGCCCAC GCAGTGTACC 
ATGCTGTACT CACGCCAGGG GACCACTGAG ACCATCGAGG AGGTGGAGGC TGAGCAGGAG 
GAGGAGGCAG GGAGCACGGC GCCTGAGCCC AGGGAGGCCA AGGAGTACGA GGCCACTGGG 
TACGATGTGG GAGCCATGGG TGCCGAGGAG GCCAGCCTCA CCCCAGAGGA AGAGCTGACA 
CAGTTCTCCA CCTTGGACGG GGATGTGGAG GCCCCACCCT CCTACAGCAA GGCTGTGAGC 
TTCGAGCATC TGTCCTTCGG CTCGCAGGAC GACTCTGCAG GCAAGAACCG TATGGCAGTC 
AGCCCGGACG ACAGCCGCAC CGACAAGCTG GGGTCCAGCA TCTTACCTCC CCTGACCCAT 
GAGCTGACGG CCAGCGAGCT GCTGCTGAAA AAGATGTTTC ACGACGATGA GCTTGAAGAG 
TCAGAGAAAT TCTACGTGGG GCAGCCCCGA TTTCTGCTGC TCTTCTATGC CATGTACAAT 
ACCCTGGTGG CCCGCTCGGA GATGGTGTGC TACTTCGTGA TCATCCTCAA CCACATGGTC 
TCTGCCTCCA TGATCACGCT CCTGCTTCCC ATCCTCATCT TCCTCTGGGC CATGTTGTCC 
GTCCCCAGGC CCAGCCGCCG GTTCTGGATG ATGGCCATCG TCTATACTGA GTGCCATGGC 
TTATGGGATG AAGATGACAT GACTGAAAGT GGCATGGCCA GGGAGGAATC AGATGATGAG 
CTCTCCCTCG GTCATGGCAG GAGGGACTCC TCCGATTCTC TCAAGTCCAT CAACCTGGCC 
GCGTCTGTGG AGTCAGTGCA TGTGACCTTC CCGGAGCAGC AGACAGCTGT CCGGAGGAAG 
CGCTCCGGCA GCAGCTCCGA GCCATCCCAG AGATCCAGCT TTTCTTCAAA CAGATCCCAA 
AGAGGCAGCA CAAGCACCCG AAACAGCAGT CAAAAAGGAA GCAGTGTTTT GAGTATTAAG 
CAAAAAGGCA AAAGGGAACT TTATATGGAA AAGCTTCAAG AACATTTAAT CAAAGCAAAA 
GCCTTTACCA TAAAGAAGAC GCTGGAGATC TATGTGCCCA TCAAACAGTT CTTTTACAAC 
CTCATCCACC CGGAGTATAG CGCCGTGACT GACGTGTATG TACTCATGTT CCTGGCTGAC 
ACTGTGGACT TCATCATCAT TGTCTTCGGC TTTTGGGCCT TTGGGAAACA CTCAGCAGCT 
GCAGACATCA CCTCTTCACT GTCAGAGGAC CAGGTCCCGG GGCCGTTTTT GGTGATGGTC 
CTCATTCAGT TTGGAACCAT GGTGGTGGAC CGAGCCCTCT ACCTCAGGAA GACTGTACTG 
GGAAAGGTCA TCTTCCAGGT CATTCTTGTG TTCGGAATTC ACTTCTGGAT GTTCTTCATC 
TTACCTGGTG TGACTGAGAG GAAATTCAGC CAGAACCTGG TTGCCCAGCT TTGGTACTTT 
GTGAAATGTG TTTACTTCGG GTTGTCTGCT TACCAGATCC GTTGTGGCTA CCCAACGCGA 
GTCCTGGGGA ACTTCCTCAC CAAGAGCTAC AATTACGTCA ACCTCTTCTT ATTCCAAGGG 
TTTCGCCTCG TGCCCTTTTT GACTGAGCTG AGGGCAGTGA TGGACTGGGT GTGGACGGAC 
ACAACTTTGA GCCTGTCCAG CTGGATCTGT GTGGAGGACA TCTATGCTCA CATATTCATC 
CTGAAGTGTT GGCGGGAGTC GGAGAAGAGA TACCCTCAGC CACGGGGCCA GAAGAAGAAG 
AAAGTGGTGA AGTATGGCAT GGGAGGAATG ATCATCGTCC TGCTCATCTG CATTGTCTGG 
TTTCCTCTTC TCTTCATGTC TTTGATCAAA TCTGTGGCTG GGGTCATCAA CCAGCCCCTG 
GACGTCTCCG TCACAATTAC CCTGGGAGGG TATCAGCCTA TTTTCACAAT GAGTGCCCAA 
CAAAGCCAGT TGAAAGTTAT GGACCAGCAG AGCTTTAACA AATTTATACA AGCTTTTTCT 
AGGGACACCG GTGCTATGCA ATTTCTGGAA AATTATGAAA AAGAAGACAT AACAGTAGCA 
GAACTGGAAG GAAACTCAAA TTCTTTGTGG ACCATCAGCC CACCCAGTAA GCAGAAAATG 
ATACACGAAC TCCTGGACCC CAATAGTAGC TTCTCTGTTG TTTTTTCATG GAGTATTCAG 
AGAAACTTAA GTCTGGGTGC AAAATCGGAA ATAGCAACAG ATAAGCTTTC TTTTCCTCTT 
AAAAATATTA CTCGAAAGAA TATCGCTAAA ATGATAGCAG GCAACAGCAC AGAAAGTTCA 
AAAACACCAG TGACCATAGA AAAGATTTAT CCATATTATG TGAAAGCACC TAGTGATTCT 
AACTCAAAAC CTATAAAGCA ACTTTTATCT GAAAATAATT TCATGGATAT TACCATCATT 
TTGTCCAGAG ACAATACAAC TAAATATAAC AGTGAGTGGT GGGTTCTCAA CCTGACTGGA 
AACAGAATAT ACAATCCGAA CTCTCAGGCC CTGGAACTGG TGGTCTTCAA TGACAAAGTC 
AGTCCCCCAA GTCTGGGGTT CCTGGCTGGC TATGGTATTA TGGGATTATA TGCTTCAGTT 
GTCCTTGTGA TTGGGAAATT TGTCCGTGAA TTCTTCAGTG GGATTTCTCA CTCCATCATG 
TTTGAAGAGC TTCCAAATGT GGATCGAATT TTGAAGTTGT GCACAGATAT TTTTTTAGTT 
CGAGAGACAG GAGAACTGGA GCTAGAAGAA GATCTCTATG CCAAATTAAT ATTCCTATAT 
CGCTCACCAG AGACAATGAT CAAATGGACT AGAGAAAAAA CAAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524028.1
CDS	998..9205
Translation	MASEVVCGLIFRLLLPICLAVACAFRYNGLSFVYLIYLLLIPLFSEPTKTTMQGHTGRLLKSLCFISLSFLLLHIIFHITLVSLEAQHRIAPGYNCSTWEKTFRQIGFESLKGADAGNGIRVFVPDIGMFIASLTIWLLCRNIVQKPVTDEAAQSNPEFENEELAEGEKIDSEEALIYEEDFNGGDGVEGELEESTKLKMFRRLASVASKLKEFIGNMITTAGKVVVTILLGSSGMMLPSLTSSVYFFVFLGLCTWWSWCRTFDPLLFSCLCVLLAIFTAGHLIGLYLYQFQFFQEAVPPNDYYARLFGIKSVIQTDCSSTWKIIVNPDLSWYHHANPILLLVMYYTLATLIRIWLQEPLVQDEGTKEEDKALACSPIQITAGRRRSLWYATHYPTDERKLLSMTQDDYKPSDGLLVTVNGNPVDYHTIHPSLPMENGPGKADLYSTPQYRWEPSDESSEKREEEEEEKEEFEEERSREEKRSIKVHAMVSVFQFIMKQSYICALIAMMAWSITYHSWLTFVLLIWSCTLWMIRNRRKYAMISSPFMVVYGNLLLILQYIWSFELPEIKKVPGFLEKKEPGELASKILFTITFWLLLRQHLTEQKALQEKEALLSEVKIGSQENEEKDEELQDIQVEGEPKEEEEEEAKEEKQERKKVEQEEAEEEDEQDIMKVLGNLVVAMFIKYWIYVCGGMFFFVSFEGKIVMYKIIYMVLFLFCVALYQVHYEWWRKILKYFWMSVVIYTMLVLIFIYTYQFENFPGLWQNMTGLKKEKLEDLGLKQFTVAELFTRIFIPTSFLLVCILHLHYFHDRFLELTDLKSIPSKEDNTIYSHAKVNGRVYLIINRISHSLPTVANRQFASVTLSIKKKLPIHQNELAHPEGSLPDLTMMHLTASLEKPEVRKLAEPGEEKLEGYSEKAQKGDLGKDSEESEEDGEEEEESEEEEETSDLRNKWHLVIDRLTVLFLKFLEYFHKLQVFMWWILELHIIKIVSSYIIWVSVKEVSLFNYVFLISWAFALPYAKLRRLASSVCTVWTCVIIVCKMLYQLQTIKPENFSVNCSLPNENQTNIPFNELNKSLLYSAPIDPTEWVGLRKSSPLLVYLRNNLLMLAILAFEVTIYRHQEYYRGRNNLTAPVSRTIFHDITRLHLDDGLINCAKYFINYFFYKFGLETCFLMSVNVIGQRMDFYAMIHACWLIAVLYRRRRKAIAEIWPKYCCFLACIITFQYFICIGIPPAPCRDYPWRFKGASFNDNIIKWLYFPDFIVRPNPVFLVYDFMLLLCASLQRQIFEDENKAAVRIMAGDNVEICMNLDAASFSQHNPVPDFIHCRSYLDMSKVIIFSYLFWFVLTIIFITGTTRISIFCMGYLVACFYFLLFGGDLLLKPIKSILRYWDWLIAYNVFVITMKNILSIGACGYIGTLVHNSCWLIQAFSLACTVKGYQMPAANSPCTLPSGEAGIIWDSICFAFLLLQRRVFMSYYFLHVVADIKASQILASRGAELFQATIVKAVKARIEEEKKSMDQLKRQMDRIKARQQKYKKGKERMLSLTQEPGEGQDMQKLSEEDDEREADKQKAKGKKKQWWRPWVDHASMVRSGDYYLFETDSEEEEEEELKKEDEEPPRRSAFQFVYQAWITDPKTALRQRHKEKKRSAREERKRRRKGSKEGPVEWEDREDEPIKKKSDGPDNIIKRIFNILKFTWVLFLATVDSFTTWLNSISREHIDISTVLRIERCMLTREIKKGNVPTRESIHMYYQNHIMNLSRESGLDTIDEHPGAASGAQTAHRMDSLDSHDSISSEPTQCTMLYSRQGTTETIEEVEAEQEEEAGSTAPEPREAKEYEATGYDVGAMGAEEASLTPEEELTQFSTLDGDVEAPPSYSKAVSFEHLSFGSQDDSAGKNRMAVSPDDSRTDKLGSSILPPLTHELTASELLLKKMFHDDELEESEKFYVGQPRFLLLFYAMYNTLVARSEMVCYFVIILNHMVSASMITLLLPILIFLWAMLSVPRPSRRFWMMAIVYTECHGLWDEDDMTESGMAREESDDELSLGHGRRDSSDSLKSINLAASVESVHVTFPEQQTAVRRKRSGSSSEPSQRSSFSSNRSQRGSTSTRNSSQKGSSVLSIKQKGKRELYMEKLQEHLIKAKAFTIKKTLEIYVPIKQFFYNLIHPEYSAVTDVYVLMFLADTVDFIIIVFGFWAFGKHSAAADITSSLSEDQVPGPFLVMVLIQFGTMVVDRALYLRKTVLGKVIFQVILVFGIHFWMFFILPGVTERKFSQNLVAQLWYFVKCVYFGLSAYQIRCGYPTRVLGNFLTKSYNYVNLFLFQGFRLVPFLTELRAVMDWVWTDTTLSLSSWICVEDIYAHIFILKCWRESEKRYPQPRGQKKKKVVKYGMGGMIIVLLICIVWFPLLFMSLIKSVAGVINQPLDVSVTITLGGYQPIFTMSAQQSQLKVMDQQSFNKFIQAFSRDTGAMQFLENYEKEDITVAELEGNSNSLWTISPPSKQKMIHELLDPNSSFSVVFSWSIQRNLSLGAKSEIATDKLSFPLKNITRKNIAKMIAGNSTESSKTPVTIEKIYPYYVKAPSDSNSKPIKQLLSENNFMDITIILSRDNTTKYNSEWWVLNLTGNRIYNPNSQALELVVFNDKVSPPSLGFLAGYGIMGLYASVVLVIGKFVREFFSGISHSIMFEELPNVDRILKLCTDIFLVRETGELELEEDLYAKLIFLYRSPETMIKWTREKTN

Target ORF information:

RefSeq Version	XM_011525726.3
Organism	Homo sapiens(human)
Definition	Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525726.3

ORF Insert Sequence:

ATGGCCTCAG AAGTGGTGTG CGGGCTCATC TTCAGGCTGC TGCTGCCCAT CTGCCTGGCA 
GTAGCATGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC 
ATTCCTCTGT TCTCAGAACC AACAAAAACG ACGATGCAAG GACATACGGG ACGGTTATTA 
AAGTCTCTGT GCTTCATCAG TCTTTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG 
TTGGTGAGCC TTGAAGCTCA ACATCGTATT GCACCTGGCT ACAACTGCTC AACATGGGAA 
AAGACATTCC GGCAGATCGG CTTTGAAAGC TTAAAGGGAG CTGATGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCCTCTGT 
AGAAACATTG TTCAGAAACC TGTGACAGAC GAAGCAGCAC AGAGTAACCC GGAGTTTGAA 
AATGAAGAAT TGGCTGAAGG AGAAAAAATT GATTCAGAAG AGGCACTGAT CTATGAAGAG 
GATTTCAATG GAGGAGATGG TGTTGAAGGC GAGTTGGAAG AAAGCACGAA GTTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCCTCTAAG CTCAAGGAGT TCATTGGCAA CATGATCACC 
ACTGCTGGGA AAGTCGTTGT TACCATCTTA CTGGGCTCCT CGGGCATGAT GTTGCCGTCT 
TTGACATCAT CTGTGTATTT TTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACTGCT 
GGACATTTGA TTGGACTTTA TTTATACCAG TTCCAATTCT TTCAAGAGGC AGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAGTCAGTAA TTCAAACGGA CTGTTCAAGT 
ACTTGGAAGA TCATAGTGAA CCCGGACCTG TCGTGGTACC ACCACGCCAA CCCTATCCTC 
CTGCTGGTGA TGTACTACAC TCTGGCCACT CTGATCCGCA TCTGGCTGCA AGAGCCCCTT 
GTGCAGGATG AGGGGACCAA AGAAGAGGAC AAAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACAGCGGGGA GGAGGCGGAG CCTGTGGTAC GCAACCCATT ACCCCACTGA TGAGAGAAAA 
CTTTTATCCA TGACCCAGGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC 
GGCAACCCCG TGGATTACCA CACCATCCAC CCAAGCCTGC CCATGGAGAA CGGCCCTGGC 
AAAGCCGACC TCTACTCCAC CCCTCAGTAC CGGTGGGAGC CCTCTGATGA ATCCTCAGAA 
AAGCGAGAGG AGGAAGAGGA AGAGAAAGAA GAATTTGAAG AAGAAAGGAG CCGTGAGGAA 
AAAAGAAGTA TCAAAGTTCA TGCCATGGTC TCCGTATTCC AATTTATTAT GAAACAAAGT 
TACATCTGTG CCCTCATAGC TATGATGGCC TGGAGCATCA CCTATCACAG CTGGCTGACC 
TTCGTGCTGC TGATCTGGTC GTGCACTCTT TGGATGATTC GCAACAGAAG AAAATATGCC 
ATGATCAGCT CTCCCTTCAT GGTGGTTTAT GGAAACCTAT TGTTGATATT ACAGTATATA 
TGGAGTTTTG AACTTCCTGA AATTAAAAAA GTTCCAGGAT TTTTAGAAAA GAAAGAGCCA 
GGAGAACTTG CTTCCAAAAT CCTTTTCACC ATTACTTTTT GGCTACTGCT GAGGCAGCAC 
CTCACAGAGC AAAAAGCTCT GCAAGAAAAG GAAGCTCTTT TATCGGAAGT CAAAATTGGC 
AGTCAGGAAA ATGAAGAAAA AGATGAGGAA CTTCAAGATA TACAAGTGGA AGGAGAGCCC 
AAAGAGGAGG AAGAAGAGGA AGCGAAGGAA GAGAAGCAAG AGAGAAAGAA GGTAGAGCAA 
GAGGAAGCTG AAGAAGAAGA TGAGCAGGAC ATCATGAAAG TCCTGGGCAA TCTGGTGGTG 
GCCATGTTCA TCAAGTACTG GATCTACGTC TGCGGAGGCA TGTTCTTCTT CGTCAGCTTC 
GAGGGTAAAA TCGTAATGTA CAAAATCATC TACATGGTGC TGTTCCTGTT CTGTGTGGCC 
CTATACCAGG TGCACTATGA ATGGTGGAGG AAAATTCTAA AATATTTTTG GATGTCAGTG 
GTTATTTACA CTATGCTGGT GCTTATCTTT ATATACACAT ATCAGTTTGA GAACTTCCCA 
GGCCTGTGGC AAAATATGAC TGGACTGAAA AAAGAAAAGC TTGAGGATCT TGGCTTAAAG 
CAGTTTACTG TGGCTGAACT ATTCACTCGC ATATTCATCC CAACCTCCTT TCTGCTGGTG 
TGCATTTTAC ACCTGCACTA CTTCCATGAC CGGTTCCTTG AACTCACAGA CCTCAAGTCC 
ATTCCCAGCA AAGAAGACAA CACCATCTAC AGCCATGCCA AAGTCAATGG TCGCGTATAT 
CTAATAATAA ATAGGATCTC ACACAGTCTT CCCACGGTTG CCAATCGACA GTTCGCATCA 
GTAACTCTCA GCATCAAGAA AAAATTACCA ATCCACCAAA ATGAACTGGC CCACCCGGAA 
GGAAGCCTCC CGGACCTCAC CATGATGCAT CTGACTGCCA GCCTGGAGAA GCCGGAGGTG 
AGGAAGTTGG CTGAGCCTGG GGAGGAGAAG CTTGAGGGCT ACTCTGAAAA AGCCCAGAAG 
GGTGATCTTG GGAAAGACAG CGAGGAGTCA GAGGAGGACG GAGAGGAAGA GGAGGAATCC 
GAGGAGGAGG AAGAAACATC AGACTTAAGG AACAAATGGC ACCTGGTGAT TGACCGCCTC 
ACTGTGCTCT TCTTAAAATT CCTGGAGTAT TTTCACAAGC TGCAGGTGTT CATGTGGTGG 
ATTTTGGAGT TGCACATCAT CAAAATCGTT TCCTCTTACA TTATCTGGGT TTCTGTGAAA 
GAGGTGTCTC TGTTCAACTA TGTATTTTTG ATTTCTTGGG CTTTTGCTCT GCCGTACGCC 
AAGCTGCGCC GTCTGGCTTC AAGTGTCTGC ACAGTCTGGA CGTGTGTGAT CATCGTCTGC 
AAAATGTTGT ACCAGCTCCA AACCATTAAG CCTGAGAACT TCTCTGTTAA CTGTTCCTTG 
CCAAATGAAA ATCAAACAAA CATCCCCTTT AATGAGTTGA ACAAGTCTCT GCTCTACAGC 
GCTCCTATCG ATCCTACAGA GTGGGTCGGC CTGCGGAAGT CTTCGCCTCT GCTAGTCTAC 
CTGAGGAATA ACCTCCTGAT GCTGGCTATC CTGGCCTTTG AAGTCACCAT TTACCGCCAT 
CAGGAATACT ATCGAGGTCG AAATAACCTG ACGGCCCCTG TGTCTAGAAC TATCTTTCAT 
GACATTACAA GACTACATCT AGATGATGGA CTTATTAATT GTGCCAAATA TTTCATTAAT 
TACTTCTTTT ACAAGTTTGG TCTGGAGACC TGTTTCCTAA TGTCAGTTAA CGTCATTGGC 
CAGCGAATGG ATTTCTATGC CATGATCCAC GCCTGCTGGC TGATCGCTGT CTTATATAGA 
CGCAGAAGGA AAGCCATCGC AGAGATCTGG CCCAAGTACT GCTGCTTCCT GGCATGCATC 
ATCACCTTCC AGTATTTCAT CTGCATTGGC ATCCCACCTG CTCCTTGCCG AGATTACCCG 
TGGAGATTCA AGGGTGCCAG CTTCAATGAC AACATCATAA AGTGGCTGTA CTTCCCAGAT 
TTCATTGTGC GGCCCAACCC TGTGTTTCTC GTCTATGACT TCATGCTGCT TCTGTGTGCC 
TCCTTACAAC GGCAGATTTT TGAGGATGAG AACAAGGCTG CAGTGCGAAT CATGGCAGGT 
GACAATGTCG AGATCTGCAT GAACCTTGAT GCGGCCTCCT TCAGCCAACA TAACCCTGTG 
CCAGATTTTA TTCACTGCAG ATCTTACTTA GACATGTCCA AAGTGATCAT CTTCAGCTAC 
CTCTTCTGGT TTGTGCTCAC CATCATCTTC ATCACTGGGA CCACCAGGAT CAGCATCTTT 
TGCATGGGGT ACCTGGTGGC CTGTTTCTAC TTCCTGCTCT TTGGGGGCGA TTTGCTGTTG 
AAACCCATCA AGAGCATCCT GCGCTACTGG GACTGGCTGA TCGCATACAA CGTTTTTGTG 
ATTACGATGA AAAATATCCT GTCAATAGGA GCATGTGGAT ACATTGGAAC ATTGGTGCAC 
AATAGTTGTT GGTTGATCCA GGCTTTCAGC CTGGCCTGCA CAGTCAAAGG CTATCAAATG 
CCTGCTGCTA ATTCACCCTG TACACTTCCC AGTGGGGAAG CAGGAATCAT TTGGGACAGC 
ATATGTTTTG CCTTCCTCCT GCTGCAAAGA AGAGTTTTCA TGAGTTATTA TTTTCTACAT 
GTTGTGGCTG ATATAAAAGC TTCCCAGATT CTGGCATCAA GAGGAGCTGA ACTTTTCCAG 
GCCACAATTG TAAAAGCTGT AAAGGCAAGA ATTGAGGAAG AGAAGAAGTC CATGGACCAG 
CTGAAGCGAC AGATGGATCG CATCAAGGCC AGGCAACAGA AATATAAAAA GGGTAAGGAG 
AGGATGCTGA GCTTGACCCA GGAGCCAGGG GAAGGCCAGG ACATGCAAAA ACTCTCTGAA 
GAGGATGATG AAAGAGAAGC AGACAAACAG AAAGCCAAGG GCAAAAAAAA GCAGTGGTGG 
CGGCCTTGGG TTGATCATGC TTCCATGGTC AGGAGTGGAG ATTATTATTT GTTTGAAACG 
GATAGTGAAG AGGAGGAAGA GGAAGAATTA AAGAAGGAAG ATGAAGAACC TCCACGAAGG 
TCAGCATTCC AGTTTGTTTA TCAAGCCTGG ATTACTGATC CTAAAACAGC ACTCCGACAA 
AGACACAAAG AGAAAAAAAG GTCTGCAAGA GAAGAACGGA AACGAAGGCG GAAAGGATCC 
AAGGAGGGTC CTGTGGAATG GGAAGACCGG GAGGATGAAC CAATCAAAAA GAAATCCGAT 
GGACCAGATA ATATCATCAA GAGGATATTT AATATTTTGA AATTTACCTG GGTCCTATTT 
CTGGCAACAG TGGACAGTTT CACTACTTGG CTTAACTCCA TTTCAAGGGA GCATATTGAT 
ATATCTACAG TTCTGAGAAT TGAACGATGC ATGCTGACCA GAGAAATTAA GAAGGGCAAT 
GTTCCAACTC GGGAGAGCAT CCACATGTAC TATCAGAACC ACATCATGAA CCTTTCCAGA 
GAGTCGGGAC TGGACACCAT TGACGAGCAT CCCGGAGCTG CTTCAGGTGC ACAGACAGCC 
CACAGGATGG ATAGTTTAGA TTCACATGAC AGTATCTCCA GCGAGCCCAC GCAGTGTACC 
ATGCTGTACT CACGCCAGGG GACCACTGAG ACCATCGAGG AGGTGGAGGC TGAGCAGGAG 
GAGGAGGCAG GGAGCACGGC GCCTGAGCCC AGGGAGGCCA AGGAGTACGA GGCCACTGGG 
TACGATGTGG GAGCCATGGG TGCCGAGGAG GCCAGCCTCA CCCCAGAGGA AGAGCTGACA 
CAGTTCTCCA CCTTGGACGG GGATGTGGAG GCCCCACCCT CCTACAGCAA GGCTGTGAGC 
TTCGAGCATC TGTCCTTCGG CTCGCAGGAC GACTCTGCAG GCAAGAACCG TATGGCAGTC 
AGCCCGGACG ACAGCCGCAC CGACAAGCTG GGGTCCAGCA TCTTACCTCC CCTGACCCAT 
GAGCTGACGG CCAGCGAGCT GCTGCTGAAA AAGATGTTTC ACGACGATGA GCTTGAAGAG 
TCAGAGAAAT TCTACGTGGG GCAGCCCCGA TTTCTGCTGC TCTTCTATGC CATGTACAAT 
ACCCTGGTGG CCCGCTCGGA GATGGTGTGC TACTTCGTGA TCATCCTCAA CCACATGGTC 
TCTGCCTCCA TGATCACGCT CCTGCTTCCC ATCCTCATCT TCCTCTGGGC CATGTTGTCC 
GTCCCCAGGC CCAGCCGCCG GTTCTGGATG ATGGCCATCG TCTATACTGA GTGCCATGGC 
TTATGGGATG AAGATGACAT GACTGAAAGT GGCATGGCCA GGGAGGAATC AGATGATGAG 
CTCTCCCTCG GTCATGGCAG GAGGGACTCC TCCGATTCTC TCAAGTCCAT CAACCTGGCC 
GCGTCTGTGG AGTCAGTGCA TGTGACCTTC CCGGAGCAGC AGACAGCTGT CCGGAGGAAG 
CGCTCCGGCA GCAGCTCCGA GCCATCCCAG AGATCCAGCT TTTCTTCAAA CAGATCCCAA 
AGAGGCAGCA CAAGCACCCG AAACAGCAGT CAAAAAGGAA GCAGTGTTTT GAGTATTAAG 
CAAAAAGGCA AAAGGGAACT TTATATGGAA AAGCTTCAAG AACATTTAAT CAAAGCAAAA 
GCCTTTACCA TAAAGAAGAC GCTGGAGATC TATGTGCCCA TCAAACAGTT CTTTTACAAC 
CTCATCCACC CGGAGTATAG CGCCGTGACT GACGTGTATG TACTCATGTT CCTGGCTGAC 
ACTGTGGACT TCATCATCAT TGTCTTCGGC TTTTGGGCCT TTGGGAAACA CTCAGCAGCT 
GCAGACATCA CCTCTTCACT GTCAGAGGAC CAGGTCCCGG GGCCGTTTTT GGTGATGGTC 
CTCATTCAGT TTGGAACCAT GGTGGTGGAC CGAGCCCTCT ACCTCAGGAA GACTGTACTG 
GGAAAGGTCA TCTTCCAGGT CATTCTTGTG TTCGGAATTC ACTTCTGGAT GTTCTTCATC 
TTACCTGGTG TGACTGAGAG GAAATTCAGC CAGAACCTGG TTGCCCAGCT TTGGTACTTT 
GTGAAATGTG TTTACTTCGG GTTGTCTGCT TACCAGATCC GTTGTGGCTA CCCAACGCGA 
GTCCTGGGGA ACTTCCTCAC CAAGAGCTAC AATTACGTCA ACCTCTTCTT ATTCCAAGGG 
TTTCGCCTCG TGCCCTTTTT GACTGAGCTG AGGGCAGTGA TGGACTGGGT GTGGACGGAC 
ACAACTTTGA GCCTGTCCAG CTGGATCTGT GTGGAGGACA TCTATGCTCA CATATTCATC 
CTGAAGTGTT GGCGGGAGTC GGAGAAGAGA TACCCTCAGC CACGGGGCCA GAAGAAGAAG 
AAAGTGGTGA AGTATGGCAT GGGAGGAATG ATCATCGTCC TGCTCATCTG CATTGTCTGG 
TTTCCTCTTC TCTTCATGTC TTTGATCAAA TCTGTGGCTG GGGTCATCAA CCAGCCCCTG 
GACGTCTCCG TCACAATTAC CCTGGGAGGG TATCAGCCTA TTTTCACAAT GAGTGCCCAA 
CAAAGCCAGT TGAAAGTTAT GGACCAGCAG AGCTTTAACA AATTTATACA AGCTTTTTCT 
AGGGACACCG GTGCTATGCA ATTTCTGGAA AATTATGAAA AAGAAGACAT AACAGTAGCA 
GAACTGGAAG GAAACTCAAA TTCTTTGTGG ACCATCAGCC CACCCAGTAA GCAGAAAATG 
ATACACGAAC TCCTGGACCC CAATAGTAGC TTCTCTGTTG TTTTTTCATG GAGTATTCAG 
AGAAACTTAA GTCTGGGTGC AAAATCGGAA ATAGCAACAG ATAAGCTTTC TTTTCCTCTT 
AAAAATATTA CTCGAAAGAA TATCGCTAAA ATGATAGCAG GCAACAGCAC AGAAAGTTCA 
AAAACACCAG TGACCATAGA AAAGATTTAT CCATATTATG TGAAAGCACC TAGTGATTCT 
AACTCAAAAC CTATAAAGCA ACTTTTATCT GAAAATAATT TCATGGATAT TACCATCATT 
TTGTCCAGAG ACAATACAAC TAAATATAAC AGTGAGTGGT GGGTTCTCAA CCTGACTGGA 
AACAGAATAT ACAATCCGAA CTCTCAGGCC CTGGAACTGG TGGTCTTCAA TGACAAAGTC 
AGTCCCCCAA GTCTGGGGTT CCTGGCTGGC TATGGTATTA TGGGATTATA TGCTTCAGTT 
GTCCTTGTGA TTGGGAAATT TGTCCGTGAA TTCTTCAGTG GGATTTCTCA CTCCATCATG 
TTTGAAGAGC TTCCAAATGT GGATCGAATT TTGAAGTTGT GCACAGATAT TTTTTTAGTT 
CGAGAGACAG GAGAACTGGA GCTAGAAGAA GATCTCTATG CCAAATTAAT ATTCCTATAT 
CGCTCACCAG AGACAATGAT CAAATGGACT AGAGAAAAAA CAAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIEZO2 ( XM_011525726.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524028.1 Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X5, mRNA.

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CloneID	OHu71134
Clone ID Related Accession (Same CDS sequence)	XM_011525726.3 , XM_011525726.2
Accession Version	XM_011525726.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	piezo-type mechanosensitive ion channel component 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525726.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG AAGTGGTGTG CGGGCTCATC TTCAGGCTGC TGCTGCCCAT CTGCCTGGCA 
GTAGCATGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC 
ATTCCTCTGT TCTCAGAACC AACAAAAACG ACGATGCAAG GACATACGGG ACGGTTATTA 
AAGTCTCTGT GCTTCATCAG TCTTTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG 
TTGGTGAGCC TTGAAGCTCA ACATCGTATT GCACCTGGCT ACAACTGCTC AACATGGGAA 
AAGACATTCC GGCAGATCGG CTTTGAAAGC TTAAAGGGAG CTGATGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCCTCTGT 
AGAAACATTG TTCAGAAACC TGTGACAGAC GAAGCAGCAC AGAGTAACCC GGAGTTTGAA 
AATGAAGAAT TGGCTGAAGG AGAAAAAATT GATTCAGAAG AGGCACTGAT CTATGAAGAG 
GATTTCAATG GAGGAGATGG TGTTGAAGGC GAGTTGGAAG AAAGCACGAA GTTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCCTCTAAG CTCAAGGAGT TCATTGGCAA CATGATCACC 
ACTGCTGGGA AAGTCGTTGT TACCATCTTA CTGGGCTCCT CGGGCATGAT GTTGCCGTCT 
TTGACATCAT CTGTGTATTT TTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACTGCT 
GGACATTTGA TTGGACTTTA TTTATACCAG TTCCAATTCT TTCAAGAGGC AGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAGTCAGTAA TTCAAACGGA CTGTTCAAGT 
ACTTGGAAGA TCATAGTGAA CCCGGACCTG TCGTGGTACC ACCACGCCAA CCCTATCCTC 
CTGCTGGTGA TGTACTACAC TCTGGCCACT CTGATCCGCA TCTGGCTGCA AGAGCCCCTT 
GTGCAGGATG AGGGGACCAA AGAAGAGGAC AAAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACAGCGGGGA GGAGGCGGAG CCTGTGGTAC GCAACCCATT ACCCCACTGA TGAGAGAAAA 
CTTTTATCCA TGACCCAGGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC 
GGCAACCCCG TGGATTACCA CACCATCCAC CCAAGCCTGC CCATGGAGAA CGGCCCTGGC 
AAAGCCGACC TCTACTCCAC CCCTCAGTAC CGGTGGGAGC CCTCTGATGA ATCCTCAGAA 
AAGCGAGAGG AGGAAGAGGA AGAGAAAGAA GAATTTGAAG AAGAAAGGAG CCGTGAGGAA 
AAAAGAAGTA TCAAAGTTCA TGCCATGGTC TCCGTATTCC AATTTATTAT GAAACAAAGT 
TACATCTGTG CCCTCATAGC TATGATGGCC TGGAGCATCA CCTATCACAG CTGGCTGACC 
TTCGTGCTGC TGATCTGGTC GTGCACTCTT TGGATGATTC GCAACAGAAG AAAATATGCC 
ATGATCAGCT CTCCCTTCAT GGTGGTTTAT GGAAACCTAT TGTTGATATT ACAGTATATA 
TGGAGTTTTG AACTTCCTGA AATTAAAAAA GTTCCAGGAT TTTTAGAAAA GAAAGAGCCA 
GGAGAACTTG CTTCCAAAAT CCTTTTCACC ATTACTTTTT GGCTACTGCT GAGGCAGCAC 
CTCACAGAGC AAAAAGCTCT GCAAGAAAAG GAAGCTCTTT TATCGGAAGT CAAAATTGGC 
AGTCAGGAAA ATGAAGAAAA AGATGAGGAA CTTCAAGATA TACAAGTGGA AGGAGAGCCC 
AAAGAGGAGG AAGAAGAGGA AGCGAAGGAA GAGAAGCAAG AGAGAAAGAA GGTAGAGCAA 
GAGGAAGCTG AAGAAGAAGA TGAGCAGGAC ATCATGAAAG TCCTGGGCAA TCTGGTGGTG 
GCCATGTTCA TCAAGTACTG GATCTACGTC TGCGGAGGCA TGTTCTTCTT CGTCAGCTTC 
GAGGGTAAAA TCGTAATGTA CAAAATCATC TACATGGTGC TGTTCCTGTT CTGTGTGGCC 
CTATACCAGG TGCACTATGA ATGGTGGAGG AAAATTCTAA AATATTTTTG GATGTCAGTG 
GTTATTTACA CTATGCTGGT GCTTATCTTT ATATACACAT ATCAGTTTGA GAACTTCCCA 
GGCCTGTGGC AAAATATGAC TGGACTGAAA AAAGAAAAGC TTGAGGATCT TGGCTTAAAG 
CAGTTTACTG TGGCTGAACT ATTCACTCGC ATATTCATCC CAACCTCCTT TCTGCTGGTG 
TGCATTTTAC ACCTGCACTA CTTCCATGAC CGGTTCCTTG AACTCACAGA CCTCAAGTCC 
ATTCCCAGCA AAGAAGACAA CACCATCTAC AGCCATGCCA AAGTCAATGG TCGCGTATAT 
CTAATAATAA ATAGGATCTC ACACAGTCTT CCCACGGTTG CCAATCGACA GTTCGCATCA 
GTAACTCTCA GCATCAAGAA AAAATTACCA ATCCACCAAA ATGAACTGGC CCACCCGGAA 
GGAAGCCTCC CGGACCTCAC CATGATGCAT CTGACTGCCA GCCTGGAGAA GCCGGAGGTG 
AGGAAGTTGG CTGAGCCTGG GGAGGAGAAG CTTGAGGGCT ACTCTGAAAA AGCCCAGAAG 
GGTGATCTTG GGAAAGACAG CGAGGAGTCA GAGGAGGACG GAGAGGAAGA GGAGGAATCC 
GAGGAGGAGG AAGAAACATC AGACTTAAGG AACAAATGGC ACCTGGTGAT TGACCGCCTC 
ACTGTGCTCT TCTTAAAATT CCTGGAGTAT TTTCACAAGC TGCAGGTGTT CATGTGGTGG 
ATTTTGGAGT TGCACATCAT CAAAATCGTT TCCTCTTACA TTATCTGGGT TTCTGTGAAA 
GAGGTGTCTC TGTTCAACTA TGTATTTTTG ATTTCTTGGG CTTTTGCTCT GCCGTACGCC 
AAGCTGCGCC GTCTGGCTTC AAGTGTCTGC ACAGTCTGGA CGTGTGTGAT CATCGTCTGC 
AAAATGTTGT ACCAGCTCCA AACCATTAAG CCTGAGAACT TCTCTGTTAA CTGTTCCTTG 
CCAAATGAAA ATCAAACAAA CATCCCCTTT AATGAGTTGA ACAAGTCTCT GCTCTACAGC 
GCTCCTATCG ATCCTACAGA GTGGGTCGGC CTGCGGAAGT CTTCGCCTCT GCTAGTCTAC 
CTGAGGAATA ACCTCCTGAT GCTGGCTATC CTGGCCTTTG AAGTCACCAT TTACCGCCAT 
CAGGAATACT ATCGAGGTCG AAATAACCTG ACGGCCCCTG TGTCTAGAAC TATCTTTCAT 
GACATTACAA GACTACATCT AGATGATGGA CTTATTAATT GTGCCAAATA TTTCATTAAT 
TACTTCTTTT ACAAGTTTGG TCTGGAGACC TGTTTCCTAA TGTCAGTTAA CGTCATTGGC 
CAGCGAATGG ATTTCTATGC CATGATCCAC GCCTGCTGGC TGATCGCTGT CTTATATAGA 
CGCAGAAGGA AAGCCATCGC AGAGATCTGG CCCAAGTACT GCTGCTTCCT GGCATGCATC 
ATCACCTTCC AGTATTTCAT CTGCATTGGC ATCCCACCTG CTCCTTGCCG AGATTACCCG 
TGGAGATTCA AGGGTGCCAG CTTCAATGAC AACATCATAA AGTGGCTGTA CTTCCCAGAT 
TTCATTGTGC GGCCCAACCC TGTGTTTCTC GTCTATGACT TCATGCTGCT TCTGTGTGCC 
TCCTTACAAC GGCAGATTTT TGAGGATGAG AACAAGGCTG CAGTGCGAAT CATGGCAGGT 
GACAATGTCG AGATCTGCAT GAACCTTGAT GCGGCCTCCT TCAGCCAACA TAACCCTGTG 
CCAGATTTTA TTCACTGCAG ATCTTACTTA GACATGTCCA AAGTGATCAT CTTCAGCTAC 
CTCTTCTGGT TTGTGCTCAC CATCATCTTC ATCACTGGGA CCACCAGGAT CAGCATCTTT 
TGCATGGGGT ACCTGGTGGC CTGTTTCTAC TTCCTGCTCT TTGGGGGCGA TTTGCTGTTG 
AAACCCATCA AGAGCATCCT GCGCTACTGG GACTGGCTGA TCGCATACAA CGTTTTTGTG 
ATTACGATGA AAAATATCCT GTCAATAGGA GCATGTGGAT ACATTGGAAC ATTGGTGCAC 
AATAGTTGTT GGTTGATCCA GGCTTTCAGC CTGGCCTGCA CAGTCAAAGG CTATCAAATG 
CCTGCTGCTA ATTCACCCTG TACACTTCCC AGTGGGGAAG CAGGAATCAT TTGGGACAGC 
ATATGTTTTG CCTTCCTCCT GCTGCAAAGA AGAGTTTTCA TGAGTTATTA TTTTCTACAT 
GTTGTGGCTG ATATAAAAGC TTCCCAGATT CTGGCATCAA GAGGAGCTGA ACTTTTCCAG 
GCCACAATTG TAAAAGCTGT AAAGGCAAGA ATTGAGGAAG AGAAGAAGTC CATGGACCAG 
CTGAAGCGAC AGATGGATCG CATCAAGGCC AGGCAACAGA AATATAAAAA GGGTAAGGAG 
AGGATGCTGA GCTTGACCCA GGAGCCAGGG GAAGGCCAGG ACATGCAAAA ACTCTCTGAA 
GAGGATGATG AAAGAGAAGC AGACAAACAG AAAGCCAAGG GCAAAAAAAA GCAGTGGTGG 
CGGCCTTGGG TTGATCATGC TTCCATGGTC AGGAGTGGAG ATTATTATTT GTTTGAAACG 
GATAGTGAAG AGGAGGAAGA GGAAGAATTA AAGAAGGAAG ATGAAGAACC TCCACGAAGG 
TCAGCATTCC AGTTTGTTTA TCAAGCCTGG ATTACTGATC CTAAAACAGC ACTCCGACAA 
AGACACAAAG AGAAAAAAAG GTCTGCAAGA GAAGAACGGA AACGAAGGCG GAAAGGATCC 
AAGGAGGGTC CTGTGGAATG GGAAGACCGG GAGGATGAAC CAATCAAAAA GAAATCCGAT 
GGACCAGATA ATATCATCAA GAGGATATTT AATATTTTGA AATTTACCTG GGTCCTATTT 
CTGGCAACAG TGGACAGTTT CACTACTTGG CTTAACTCCA TTTCAAGGGA GCATATTGAT 
ATATCTACAG TTCTGAGAAT TGAACGATGC ATGCTGACCA GAGAAATTAA GAAGGGCAAT 
GTTCCAACTC GGGAGAGCAT CCACATGTAC TATCAGAACC ACATCATGAA CCTTTCCAGA 
GAGTCGGGAC TGGACACCAT TGACGAGCAT CCCGGAGCTG CTTCAGGTGC ACAGACAGCC 
CACAGGATGG ATAGTTTAGA TTCACATGAC AGTATCTCCA GCGAGCCCAC GCAGTGTACC 
ATGCTGTACT CACGCCAGGG GACCACTGAG ACCATCGAGG AGGTGGAGGC TGAGCAGGAG 
GAGGAGGCAG GGAGCACGGC GCCTGAGCCC AGGGAGGCCA AGGAGTACGA GGCCACTGGG 
TACGATGTGG GAGCCATGGG TGCCGAGGAG GCCAGCCTCA CCCCAGAGGA AGAGCTGACA 
CAGTTCTCCA CCTTGGACGG GGATGTGGAG GCCCCACCCT CCTACAGCAA GGCTGTGAGC 
TTCGAGCATC TGTCCTTCGG CTCGCAGGAC GACTCTGCAG GCAAGAACCG TATGGCAGTC 
AGCCCGGACG ACAGCCGCAC CGACAAGCTG GGGTCCAGCA TCTTACCTCC CCTGACCCAT 
GAGCTGACGG CCAGCGAGCT GCTGCTGAAA AAGATGTTTC ACGACGATGA GCTTGAAGAG 
TCAGAGAAAT TCTACGTGGG GCAGCCCCGA TTTCTGCTGC TCTTCTATGC CATGTACAAT 
ACCCTGGTGG CCCGCTCGGA GATGGTGTGC TACTTCGTGA TCATCCTCAA CCACATGGTC 
TCTGCCTCCA TGATCACGCT CCTGCTTCCC ATCCTCATCT TCCTCTGGGC CATGTTGTCC 
GTCCCCAGGC CCAGCCGCCG GTTCTGGATG ATGGCCATCG TCTATACTGA GTGCCATGGC 
TTATGGGATG AAGATGACAT GACTGAAAGT GGCATGGCCA GGGAGGAATC AGATGATGAG 
CTCTCCCTCG GTCATGGCAG GAGGGACTCC TCCGATTCTC TCAAGTCCAT CAACCTGGCC 
GCGTCTGTGG AGTCAGTGCA TGTGACCTTC CCGGAGCAGC AGACAGCTGT CCGGAGGAAG 
CGCTCCGGCA GCAGCTCCGA GCCATCCCAG AGATCCAGCT TTTCTTCAAA CAGATCCCAA 
AGAGGCAGCA CAAGCACCCG AAACAGCAGT CAAAAAGGAA GCAGTGTTTT GAGTATTAAG 
CAAAAAGGCA AAAGGGAACT TTATATGGAA AAGCTTCAAG AACATTTAAT CAAAGCAAAA 
GCCTTTACCA TAAAGAAGAC GCTGGAGATC TATGTGCCCA TCAAACAGTT CTTTTACAAC 
CTCATCCACC CGGAGTATAG CGCCGTGACT GACGTGTATG TACTCATGTT CCTGGCTGAC 
ACTGTGGACT TCATCATCAT TGTCTTCGGC TTTTGGGCCT TTGGGAAACA CTCAGCAGCT 
GCAGACATCA CCTCTTCACT GTCAGAGGAC CAGGTCCCGG GGCCGTTTTT GGTGATGGTC 
CTCATTCAGT TTGGAACCAT GGTGGTGGAC CGAGCCCTCT ACCTCAGGAA GACTGTACTG 
GGAAAGGTCA TCTTCCAGGT CATTCTTGTG TTCGGAATTC ACTTCTGGAT GTTCTTCATC 
TTACCTGGTG TGACTGAGAG GAAATTCAGC CAGAACCTGG TTGCCCAGCT TTGGTACTTT 
GTGAAATGTG TTTACTTCGG GTTGTCTGCT TACCAGATCC GTTGTGGCTA CCCAACGCGA 
GTCCTGGGGA ACTTCCTCAC CAAGAGCTAC AATTACGTCA ACCTCTTCTT ATTCCAAGGG 
TTTCGCCTCG TGCCCTTTTT GACTGAGCTG AGGGCAGTGA TGGACTGGGT GTGGACGGAC 
ACAACTTTGA GCCTGTCCAG CTGGATCTGT GTGGAGGACA TCTATGCTCA CATATTCATC 
CTGAAGTGTT GGCGGGAGTC GGAGAAGAGA TACCCTCAGC CACGGGGCCA GAAGAAGAAG 
AAAGTGGTGA AGTATGGCAT GGGAGGAATG ATCATCGTCC TGCTCATCTG CATTGTCTGG 
TTTCCTCTTC TCTTCATGTC TTTGATCAAA TCTGTGGCTG GGGTCATCAA CCAGCCCCTG 
GACGTCTCCG TCACAATTAC CCTGGGAGGG TATCAGCCTA TTTTCACAAT GAGTGCCCAA 
CAAAGCCAGT TGAAAGTTAT GGACCAGCAG AGCTTTAACA AATTTATACA AGCTTTTTCT 
AGGGACACCG GTGCTATGCA ATTTCTGGAA AATTATGAAA AAGAAGACAT AACAGTAGCA 
GAACTGGAAG GAAACTCAAA TTCTTTGTGG ACCATCAGCC CACCCAGTAA GCAGAAAATG 
ATACACGAAC TCCTGGACCC CAATAGTAGC TTCTCTGTTG TTTTTTCATG GAGTATTCAG 
AGAAACTTAA GTCTGGGTGC AAAATCGGAA ATAGCAACAG ATAAGCTTTC TTTTCCTCTT 
AAAAATATTA CTCGAAAGAA TATCGCTAAA ATGATAGCAG GCAACAGCAC AGAAAGTTCA 
AAAACACCAG TGACCATAGA AAAGATTTAT CCATATTATG TGAAAGCACC TAGTGATTCT 
AACTCAAAAC CTATAAAGCA ACTTTTATCT GAAAATAATT TCATGGATAT TACCATCATT 
TTGTCCAGAG ACAATACAAC TAAATATAAC AGTGAGTGGT GGGTTCTCAA CCTGACTGGA 
AACAGAATAT ACAATCCGAA CTCTCAGGCC CTGGAACTGG TGGTCTTCAA TGACAAAGTC 
AGTCCCCCAA GTCTGGGGTT CCTGGCTGGC TATGGTATTA TGGGATTATA TGCTTCAGTT 
GTCCTTGTGA TTGGGAAATT TGTCCGTGAA TTCTTCAGTG GGATTTCTCA CTCCATCATG 
TTTGAAGAGC TTCCAAATGT GGATCGAATT TTGAAGTTGT GCACAGATAT TTTTTTAGTT 
CGAGAGACAG GAGAACTGGA GCTAGAAGAA GATCTCTATG CCAAATTAAT ATTCCTATAT 
CGCTCACCAG AGACAATGAT CAAATGGACT AGAGAAAAAA CAAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524028.1
CDS	973..9180
Translation	MASEVVCGLIFRLLLPICLAVACAFRYNGLSFVYLIYLLLIPLFSEPTKTTMQGHTGRLLKSLCFISLSFLLLHIIFHITLVSLEAQHRIAPGYNCSTWEKTFRQIGFESLKGADAGNGIRVFVPDIGMFIASLTIWLLCRNIVQKPVTDEAAQSNPEFENEELAEGEKIDSEEALIYEEDFNGGDGVEGELEESTKLKMFRRLASVASKLKEFIGNMITTAGKVVVTILLGSSGMMLPSLTSSVYFFVFLGLCTWWSWCRTFDPLLFSCLCVLLAIFTAGHLIGLYLYQFQFFQEAVPPNDYYARLFGIKSVIQTDCSSTWKIIVNPDLSWYHHANPILLLVMYYTLATLIRIWLQEPLVQDEGTKEEDKALACSPIQITAGRRRSLWYATHYPTDERKLLSMTQDDYKPSDGLLVTVNGNPVDYHTIHPSLPMENGPGKADLYSTPQYRWEPSDESSEKREEEEEEKEEFEEERSREEKRSIKVHAMVSVFQFIMKQSYICALIAMMAWSITYHSWLTFVLLIWSCTLWMIRNRRKYAMISSPFMVVYGNLLLILQYIWSFELPEIKKVPGFLEKKEPGELASKILFTITFWLLLRQHLTEQKALQEKEALLSEVKIGSQENEEKDEELQDIQVEGEPKEEEEEEAKEEKQERKKVEQEEAEEEDEQDIMKVLGNLVVAMFIKYWIYVCGGMFFFVSFEGKIVMYKIIYMVLFLFCVALYQVHYEWWRKILKYFWMSVVIYTMLVLIFIYTYQFENFPGLWQNMTGLKKEKLEDLGLKQFTVAELFTRIFIPTSFLLVCILHLHYFHDRFLELTDLKSIPSKEDNTIYSHAKVNGRVYLIINRISHSLPTVANRQFASVTLSIKKKLPIHQNELAHPEGSLPDLTMMHLTASLEKPEVRKLAEPGEEKLEGYSEKAQKGDLGKDSEESEEDGEEEEESEEEEETSDLRNKWHLVIDRLTVLFLKFLEYFHKLQVFMWWILELHIIKIVSSYIIWVSVKEVSLFNYVFLISWAFALPYAKLRRLASSVCTVWTCVIIVCKMLYQLQTIKPENFSVNCSLPNENQTNIPFNELNKSLLYSAPIDPTEWVGLRKSSPLLVYLRNNLLMLAILAFEVTIYRHQEYYRGRNNLTAPVSRTIFHDITRLHLDDGLINCAKYFINYFFYKFGLETCFLMSVNVIGQRMDFYAMIHACWLIAVLYRRRRKAIAEIWPKYCCFLACIITFQYFICIGIPPAPCRDYPWRFKGASFNDNIIKWLYFPDFIVRPNPVFLVYDFMLLLCASLQRQIFEDENKAAVRIMAGDNVEICMNLDAASFSQHNPVPDFIHCRSYLDMSKVIIFSYLFWFVLTIIFITGTTRISIFCMGYLVACFYFLLFGGDLLLKPIKSILRYWDWLIAYNVFVITMKNILSIGACGYIGTLVHNSCWLIQAFSLACTVKGYQMPAANSPCTLPSGEAGIIWDSICFAFLLLQRRVFMSYYFLHVVADIKASQILASRGAELFQATIVKAVKARIEEEKKSMDQLKRQMDRIKARQQKYKKGKERMLSLTQEPGEGQDMQKLSEEDDEREADKQKAKGKKKQWWRPWVDHASMVRSGDYYLFETDSEEEEEEELKKEDEEPPRRSAFQFVYQAWITDPKTALRQRHKEKKRSAREERKRRRKGSKEGPVEWEDREDEPIKKKSDGPDNIIKRIFNILKFTWVLFLATVDSFTTWLNSISREHIDISTVLRIERCMLTREIKKGNVPTRESIHMYYQNHIMNLSRESGLDTIDEHPGAASGAQTAHRMDSLDSHDSISSEPTQCTMLYSRQGTTETIEEVEAEQEEEAGSTAPEPREAKEYEATGYDVGAMGAEEASLTPEEELTQFSTLDGDVEAPPSYSKAVSFEHLSFGSQDDSAGKNRMAVSPDDSRTDKLGSSILPPLTHELTASELLLKKMFHDDELEESEKFYVGQPRFLLLFYAMYNTLVARSEMVCYFVIILNHMVSASMITLLLPILIFLWAMLSVPRPSRRFWMMAIVYTECHGLWDEDDMTESGMAREESDDELSLGHGRRDSSDSLKSINLAASVESVHVTFPEQQTAVRRKRSGSSSEPSQRSSFSSNRSQRGSTSTRNSSQKGSSVLSIKQKGKRELYMEKLQEHLIKAKAFTIKKTLEIYVPIKQFFYNLIHPEYSAVTDVYVLMFLADTVDFIIIVFGFWAFGKHSAAADITSSLSEDQVPGPFLVMVLIQFGTMVVDRALYLRKTVLGKVIFQVILVFGIHFWMFFILPGVTERKFSQNLVAQLWYFVKCVYFGLSAYQIRCGYPTRVLGNFLTKSYNYVNLFLFQGFRLVPFLTELRAVMDWVWTDTTLSLSSWICVEDIYAHIFILKCWRESEKRYPQPRGQKKKKVVKYGMGGMIIVLLICIVWFPLLFMSLIKSVAGVINQPLDVSVTITLGGYQPIFTMSAQQSQLKVMDQQSFNKFIQAFSRDTGAMQFLENYEKEDITVAELEGNSNSLWTISPPSKQKMIHELLDPNSSFSVVFSWSIQRNLSLGAKSEIATDKLSFPLKNITRKNIAKMIAGNSTESSKTPVTIEKIYPYYVKAPSDSNSKPIKQLLSENNFMDITIILSRDNTTKYNSEWWVLNLTGNRIYNPNSQALELVVFNDKVSPPSLGFLAGYGIMGLYASVVLVIGKFVREFFSGISHSIMFEELPNVDRILKLCTDIFLVRETGELELEEDLYAKLIFLYRSPETMIKWTREKTN

Target ORF information:

RefSeq Version	XM_011525726.2
Organism	Homo sapiens(human)
Definition	Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525726.2

ORF Insert Sequence:

ATGGCCTCAG AAGTGGTGTG CGGGCTCATC TTCAGGCTGC TGCTGCCCAT CTGCCTGGCA 
GTAGCATGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC 
ATTCCTCTGT TCTCAGAACC AACAAAAACG ACGATGCAAG GACATACGGG ACGGTTATTA 
AAGTCTCTGT GCTTCATCAG TCTTTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG 
TTGGTGAGCC TTGAAGCTCA ACATCGTATT GCACCTGGCT ACAACTGCTC AACATGGGAA 
AAGACATTCC GGCAGATCGG CTTTGAAAGC TTAAAGGGAG CTGATGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCCTCTGT 
AGAAACATTG TTCAGAAACC TGTGACAGAC GAAGCAGCAC AGAGTAACCC GGAGTTTGAA 
AATGAAGAAT TGGCTGAAGG AGAAAAAATT GATTCAGAAG AGGCACTGAT CTATGAAGAG 
GATTTCAATG GAGGAGATGG TGTTGAAGGC GAGTTGGAAG AAAGCACGAA GTTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCCTCTAAG CTCAAGGAGT TCATTGGCAA CATGATCACC 
ACTGCTGGGA AAGTCGTTGT TACCATCTTA CTGGGCTCCT CGGGCATGAT GTTGCCGTCT 
TTGACATCAT CTGTGTATTT TTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACTGCT 
GGACATTTGA TTGGACTTTA TTTATACCAG TTCCAATTCT TTCAAGAGGC AGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAGTCAGTAA TTCAAACGGA CTGTTCAAGT 
ACTTGGAAGA TCATAGTGAA CCCGGACCTG TCGTGGTACC ACCACGCCAA CCCTATCCTC 
CTGCTGGTGA TGTACTACAC TCTGGCCACT CTGATCCGCA TCTGGCTGCA AGAGCCCCTT 
GTGCAGGATG AGGGGACCAA AGAAGAGGAC AAAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACAGCGGGGA GGAGGCGGAG CCTGTGGTAC GCAACCCATT ACCCCACTGA TGAGAGAAAA 
CTTTTATCCA TGACCCAGGA TGACTACAAA CCATCTGATG GCCTGCTGGT GACTGTGAAC 
GGCAACCCCG TGGATTACCA CACCATCCAC CCAAGCCTGC CCATGGAGAA CGGCCCTGGC 
AAAGCCGACC TCTACTCCAC CCCTCAGTAC CGGTGGGAGC CCTCTGATGA ATCCTCAGAA 
AAGCGAGAGG AGGAAGAGGA AGAGAAAGAA GAATTTGAAG AAGAAAGGAG CCGTGAGGAA 
AAAAGAAGTA TCAAAGTTCA TGCCATGGTC TCCGTATTCC AATTTATTAT GAAACAAAGT 
TACATCTGTG CCCTCATAGC TATGATGGCC TGGAGCATCA CCTATCACAG CTGGCTGACC 
TTCGTGCTGC TGATCTGGTC GTGCACTCTT TGGATGATTC GCAACAGAAG AAAATATGCC 
ATGATCAGCT CTCCCTTCAT GGTGGTTTAT GGAAACCTAT TGTTGATATT ACAGTATATA 
TGGAGTTTTG AACTTCCTGA AATTAAAAAA GTTCCAGGAT TTTTAGAAAA GAAAGAGCCA 
GGAGAACTTG CTTCCAAAAT CCTTTTCACC ATTACTTTTT GGCTACTGCT GAGGCAGCAC 
CTCACAGAGC AAAAAGCTCT GCAAGAAAAG GAAGCTCTTT TATCGGAAGT CAAAATTGGC 
AGTCAGGAAA ATGAAGAAAA AGATGAGGAA CTTCAAGATA TACAAGTGGA AGGAGAGCCC 
AAAGAGGAGG AAGAAGAGGA AGCGAAGGAA GAGAAGCAAG AGAGAAAGAA GGTAGAGCAA 
GAGGAAGCTG AAGAAGAAGA TGAGCAGGAC ATCATGAAAG TCCTGGGCAA TCTGGTGGTG 
GCCATGTTCA TCAAGTACTG GATCTACGTC TGCGGAGGCA TGTTCTTCTT CGTCAGCTTC 
GAGGGTAAAA TCGTAATGTA CAAAATCATC TACATGGTGC TGTTCCTGTT CTGTGTGGCC 
CTATACCAGG TGCACTATGA ATGGTGGAGG AAAATTCTAA AATATTTTTG GATGTCAGTG 
GTTATTTACA CTATGCTGGT GCTTATCTTT ATATACACAT ATCAGTTTGA GAACTTCCCA 
GGCCTGTGGC AAAATATGAC TGGACTGAAA AAAGAAAAGC TTGAGGATCT TGGCTTAAAG 
CAGTTTACTG TGGCTGAACT ATTCACTCGC ATATTCATCC CAACCTCCTT TCTGCTGGTG 
TGCATTTTAC ACCTGCACTA CTTCCATGAC CGGTTCCTTG AACTCACAGA CCTCAAGTCC 
ATTCCCAGCA AAGAAGACAA CACCATCTAC AGCCATGCCA AAGTCAATGG TCGCGTATAT 
CTAATAATAA ATAGGATCTC ACACAGTCTT CCCACGGTTG CCAATCGACA GTTCGCATCA 
GTAACTCTCA GCATCAAGAA AAAATTACCA ATCCACCAAA ATGAACTGGC CCACCCGGAA 
GGAAGCCTCC CGGACCTCAC CATGATGCAT CTGACTGCCA GCCTGGAGAA GCCGGAGGTG 
AGGAAGTTGG CTGAGCCTGG GGAGGAGAAG CTTGAGGGCT ACTCTGAAAA AGCCCAGAAG 
GGTGATCTTG GGAAAGACAG CGAGGAGTCA GAGGAGGACG GAGAGGAAGA GGAGGAATCC 
GAGGAGGAGG AAGAAACATC AGACTTAAGG AACAAATGGC ACCTGGTGAT TGACCGCCTC 
ACTGTGCTCT TCTTAAAATT CCTGGAGTAT TTTCACAAGC TGCAGGTGTT CATGTGGTGG 
ATTTTGGAGT TGCACATCAT CAAAATCGTT TCCTCTTACA TTATCTGGGT TTCTGTGAAA 
GAGGTGTCTC TGTTCAACTA TGTATTTTTG ATTTCTTGGG CTTTTGCTCT GCCGTACGCC 
AAGCTGCGCC GTCTGGCTTC AAGTGTCTGC ACAGTCTGGA CGTGTGTGAT CATCGTCTGC 
AAAATGTTGT ACCAGCTCCA AACCATTAAG CCTGAGAACT TCTCTGTTAA CTGTTCCTTG 
CCAAATGAAA ATCAAACAAA CATCCCCTTT AATGAGTTGA ACAAGTCTCT GCTCTACAGC 
GCTCCTATCG ATCCTACAGA GTGGGTCGGC CTGCGGAAGT CTTCGCCTCT GCTAGTCTAC 
CTGAGGAATA ACCTCCTGAT GCTGGCTATC CTGGCCTTTG AAGTCACCAT TTACCGCCAT 
CAGGAATACT ATCGAGGTCG AAATAACCTG ACGGCCCCTG TGTCTAGAAC TATCTTTCAT 
GACATTACAA GACTACATCT AGATGATGGA CTTATTAATT GTGCCAAATA TTTCATTAAT 
TACTTCTTTT ACAAGTTTGG TCTGGAGACC TGTTTCCTAA TGTCAGTTAA CGTCATTGGC 
CAGCGAATGG ATTTCTATGC CATGATCCAC GCCTGCTGGC TGATCGCTGT CTTATATAGA 
CGCAGAAGGA AAGCCATCGC AGAGATCTGG CCCAAGTACT GCTGCTTCCT GGCATGCATC 
ATCACCTTCC AGTATTTCAT CTGCATTGGC ATCCCACCTG CTCCTTGCCG AGATTACCCG 
TGGAGATTCA AGGGTGCCAG CTTCAATGAC AACATCATAA AGTGGCTGTA CTTCCCAGAT 
TTCATTGTGC GGCCCAACCC TGTGTTTCTC GTCTATGACT TCATGCTGCT TCTGTGTGCC 
TCCTTACAAC GGCAGATTTT TGAGGATGAG AACAAGGCTG CAGTGCGAAT CATGGCAGGT 
GACAATGTCG AGATCTGCAT GAACCTTGAT GCGGCCTCCT TCAGCCAACA TAACCCTGTG 
CCAGATTTTA TTCACTGCAG ATCTTACTTA GACATGTCCA AAGTGATCAT CTTCAGCTAC 
CTCTTCTGGT TTGTGCTCAC CATCATCTTC ATCACTGGGA CCACCAGGAT CAGCATCTTT 
TGCATGGGGT ACCTGGTGGC CTGTTTCTAC TTCCTGCTCT TTGGGGGCGA TTTGCTGTTG 
AAACCCATCA AGAGCATCCT GCGCTACTGG GACTGGCTGA TCGCATACAA CGTTTTTGTG 
ATTACGATGA AAAATATCCT GTCAATAGGA GCATGTGGAT ACATTGGAAC ATTGGTGCAC 
AATAGTTGTT GGTTGATCCA GGCTTTCAGC CTGGCCTGCA CAGTCAAAGG CTATCAAATG 
CCTGCTGCTA ATTCACCCTG TACACTTCCC AGTGGGGAAG CAGGAATCAT TTGGGACAGC 
ATATGTTTTG CCTTCCTCCT GCTGCAAAGA AGAGTTTTCA TGAGTTATTA TTTTCTACAT 
GTTGTGGCTG ATATAAAAGC TTCCCAGATT CTGGCATCAA GAGGAGCTGA ACTTTTCCAG 
GCCACAATTG TAAAAGCTGT AAAGGCAAGA ATTGAGGAAG AGAAGAAGTC CATGGACCAG 
CTGAAGCGAC AGATGGATCG CATCAAGGCC AGGCAACAGA AATATAAAAA GGGTAAGGAG 
AGGATGCTGA GCTTGACCCA GGAGCCAGGG GAAGGCCAGG ACATGCAAAA ACTCTCTGAA 
GAGGATGATG AAAGAGAAGC AGACAAACAG AAAGCCAAGG GCAAAAAAAA GCAGTGGTGG 
CGGCCTTGGG TTGATCATGC TTCCATGGTC AGGAGTGGAG ATTATTATTT GTTTGAAACG 
GATAGTGAAG AGGAGGAAGA GGAAGAATTA AAGAAGGAAG ATGAAGAACC TCCACGAAGG 
TCAGCATTCC AGTTTGTTTA TCAAGCCTGG ATTACTGATC CTAAAACAGC ACTCCGACAA 
AGACACAAAG AGAAAAAAAG GTCTGCAAGA GAAGAACGGA AACGAAGGCG GAAAGGATCC 
AAGGAGGGTC CTGTGGAATG GGAAGACCGG GAGGATGAAC CAATCAAAAA GAAATCCGAT 
GGACCAGATA ATATCATCAA GAGGATATTT AATATTTTGA AATTTACCTG GGTCCTATTT 
CTGGCAACAG TGGACAGTTT CACTACTTGG CTTAACTCCA TTTCAAGGGA GCATATTGAT 
ATATCTACAG TTCTGAGAAT TGAACGATGC ATGCTGACCA GAGAAATTAA GAAGGGCAAT 
GTTCCAACTC GGGAGAGCAT CCACATGTAC TATCAGAACC ACATCATGAA CCTTTCCAGA 
GAGTCGGGAC TGGACACCAT TGACGAGCAT CCCGGAGCTG CTTCAGGTGC ACAGACAGCC 
CACAGGATGG ATAGTTTAGA TTCACATGAC AGTATCTCCA GCGAGCCCAC GCAGTGTACC 
ATGCTGTACT CACGCCAGGG GACCACTGAG ACCATCGAGG AGGTGGAGGC TGAGCAGGAG 
GAGGAGGCAG GGAGCACGGC GCCTGAGCCC AGGGAGGCCA AGGAGTACGA GGCCACTGGG 
TACGATGTGG GAGCCATGGG TGCCGAGGAG GCCAGCCTCA CCCCAGAGGA AGAGCTGACA 
CAGTTCTCCA CCTTGGACGG GGATGTGGAG GCCCCACCCT CCTACAGCAA GGCTGTGAGC 
TTCGAGCATC TGTCCTTCGG CTCGCAGGAC GACTCTGCAG GCAAGAACCG TATGGCAGTC 
AGCCCGGACG ACAGCCGCAC CGACAAGCTG GGGTCCAGCA TCTTACCTCC CCTGACCCAT 
GAGCTGACGG CCAGCGAGCT GCTGCTGAAA AAGATGTTTC ACGACGATGA GCTTGAAGAG 
TCAGAGAAAT TCTACGTGGG GCAGCCCCGA TTTCTGCTGC TCTTCTATGC CATGTACAAT 
ACCCTGGTGG CCCGCTCGGA GATGGTGTGC TACTTCGTGA TCATCCTCAA CCACATGGTC 
TCTGCCTCCA TGATCACGCT CCTGCTTCCC ATCCTCATCT TCCTCTGGGC CATGTTGTCC 
GTCCCCAGGC CCAGCCGCCG GTTCTGGATG ATGGCCATCG TCTATACTGA GTGCCATGGC 
TTATGGGATG AAGATGACAT GACTGAAAGT GGCATGGCCA GGGAGGAATC AGATGATGAG 
CTCTCCCTCG GTCATGGCAG GAGGGACTCC TCCGATTCTC TCAAGTCCAT CAACCTGGCC 
GCGTCTGTGG AGTCAGTGCA TGTGACCTTC CCGGAGCAGC AGACAGCTGT CCGGAGGAAG 
CGCTCCGGCA GCAGCTCCGA GCCATCCCAG AGATCCAGCT TTTCTTCAAA CAGATCCCAA 
AGAGGCAGCA CAAGCACCCG AAACAGCAGT CAAAAAGGAA GCAGTGTTTT GAGTATTAAG 
CAAAAAGGCA AAAGGGAACT TTATATGGAA AAGCTTCAAG AACATTTAAT CAAAGCAAAA 
GCCTTTACCA TAAAGAAGAC GCTGGAGATC TATGTGCCCA TCAAACAGTT CTTTTACAAC 
CTCATCCACC CGGAGTATAG CGCCGTGACT GACGTGTATG TACTCATGTT CCTGGCTGAC 
ACTGTGGACT TCATCATCAT TGTCTTCGGC TTTTGGGCCT TTGGGAAACA CTCAGCAGCT 
GCAGACATCA CCTCTTCACT GTCAGAGGAC CAGGTCCCGG GGCCGTTTTT GGTGATGGTC 
CTCATTCAGT TTGGAACCAT GGTGGTGGAC CGAGCCCTCT ACCTCAGGAA GACTGTACTG 
GGAAAGGTCA TCTTCCAGGT CATTCTTGTG TTCGGAATTC ACTTCTGGAT GTTCTTCATC 
TTACCTGGTG TGACTGAGAG GAAATTCAGC CAGAACCTGG TTGCCCAGCT TTGGTACTTT 
GTGAAATGTG TTTACTTCGG GTTGTCTGCT TACCAGATCC GTTGTGGCTA CCCAACGCGA 
GTCCTGGGGA ACTTCCTCAC CAAGAGCTAC AATTACGTCA ACCTCTTCTT ATTCCAAGGG 
TTTCGCCTCG TGCCCTTTTT GACTGAGCTG AGGGCAGTGA TGGACTGGGT GTGGACGGAC 
ACAACTTTGA GCCTGTCCAG CTGGATCTGT GTGGAGGACA TCTATGCTCA CATATTCATC 
CTGAAGTGTT GGCGGGAGTC GGAGAAGAGA TACCCTCAGC CACGGGGCCA GAAGAAGAAG 
AAAGTGGTGA AGTATGGCAT GGGAGGAATG ATCATCGTCC TGCTCATCTG CATTGTCTGG 
TTTCCTCTTC TCTTCATGTC TTTGATCAAA TCTGTGGCTG GGGTCATCAA CCAGCCCCTG 
GACGTCTCCG TCACAATTAC CCTGGGAGGG TATCAGCCTA TTTTCACAAT GAGTGCCCAA 
CAAAGCCAGT TGAAAGTTAT GGACCAGCAG AGCTTTAACA AATTTATACA AGCTTTTTCT 
AGGGACACCG GTGCTATGCA ATTTCTGGAA AATTATGAAA AAGAAGACAT AACAGTAGCA 
GAACTGGAAG GAAACTCAAA TTCTTTGTGG ACCATCAGCC CACCCAGTAA GCAGAAAATG 
ATACACGAAC TCCTGGACCC CAATAGTAGC TTCTCTGTTG TTTTTTCATG GAGTATTCAG 
AGAAACTTAA GTCTGGGTGC AAAATCGGAA ATAGCAACAG ATAAGCTTTC TTTTCCTCTT 
AAAAATATTA CTCGAAAGAA TATCGCTAAA ATGATAGCAG GCAACAGCAC AGAAAGTTCA 
AAAACACCAG TGACCATAGA AAAGATTTAT CCATATTATG TGAAAGCACC TAGTGATTCT 
AACTCAAAAC CTATAAAGCA ACTTTTATCT GAAAATAATT TCATGGATAT TACCATCATT 
TTGTCCAGAG ACAATACAAC TAAATATAAC AGTGAGTGGT GGGTTCTCAA CCTGACTGGA 
AACAGAATAT ACAATCCGAA CTCTCAGGCC CTGGAACTGG TGGTCTTCAA TGACAAAGTC 
AGTCCCCCAA GTCTGGGGTT CCTGGCTGGC TATGGTATTA TGGGATTATA TGCTTCAGTT 
GTCCTTGTGA TTGGGAAATT TGTCCGTGAA TTCTTCAGTG GGATTTCTCA CTCCATCATG 
TTTGAAGAGC TTCCAAATGT GGATCGAATT TTGAAGTTGT GCACAGATAT TTTTTTAGTT 
CGAGAGACAG GAGAACTGGA GCTAGAAGAA GATCTCTATG CCAAATTAAT ATTCCTATAT 
CGCTCACCAG AGACAATGAT CAAATGGACT AGAGAAAAAA CAAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIEZO2 ( XM_017025918.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881407.1 Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X2, mRNA.

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CloneID	OHu88005
Clone ID Related Accession (Same CDS sequence)	XM_017025918.1 , XM_017025918.2
Accession Version	XM_017025918.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	piezo-type mechanosensitive ion channel component 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG AAGTGGTGTG CGGGCTCATC TTCAGGCTGC TGCTGCCCAT CTGCCTGGCA 
GTAGCATGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC 
ATTCCTCTGT TCTCAGAACC AACAAAAACG ACGATGCAAG GACATACGGG ACGGTTATTA 
AAGTCTCTGT GCTTCATCAG TCTTTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG 
TTGGTGAGCC TTGAAGCTCA ACATCGTATT GCACCTGGCT ACAACTGCTC AACATGGGAA 
AAGACATTCC GGCAGATCGG CTTTGAAAGC TTAAAGGGAG CTGATGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCCTCTGT 
AGAAACATTG TTCAGAAACC TGTGACAGAC GAAGCAGCAC AGAGTAACCC GGAGTTTGAA 
AATGAAGAAT TGGCTGAAGG AGAAAAAATT GATTCAGAAG AGGCACTGAT CTATGAAGAG 
GATTTCAATG GAGGAGATGG TGTTGAAGGC GAGTTGGAAG AAAGCACGAA GTTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCCTCTAAG CTCAAGGAGT TCATTGGCAA CATGATCACC 
ACTGCTGGGA AAGTCGTTGT TACCATCTTA CTGGGCTCCT CGGGCATGAT GTTGCCGTCT 
TTGACATCAT CTGTGTATTT TTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACTGCT 
GGACATTTGA TTGGACTTTA TTTATACCAG TTCCAATTCT TTCAAGAGGC AGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAGTCAGTAA TTCAAACGGA CTGTTCAAGT 
ACTTGGAAGA TCATAGTGAA CCCGGACCTG TCGTGGTACC ACCACGCCAA CCCTATCCTC 
CTGCTGGTGA TGTACTACAC TCTGGCCACT CTGATCCGCA TCTGGCTGCA AGAGCCCCTT 
GTGCAGGATG AGGGGACCAA AGAAGAGGAC AAAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACAGCGGGGA GGAGGCGGAG CCTGTGGTAC GCAACCCATT ACCCCACTGA TGAGAGAAAA 
GGCCTGCTGG TGACTGTGAA CGGCAACCCC GTGGATTACC ACACCATCCA CCCAAGCCTG 
CCCATGGAGA ACGGCCCTGG CAAAGCCGAC CTCTACTCCA CCCCTCAGTA CCGGTGGGAG 
CCCTCTGATG AATCCTCAGA AAAGCGAGAG GAGGAAGAGG AAGAGAAAGA AGAATTTGAA 
GAAGAAAGGA GCCGTGAGGA AAAAAGAAGT ATCAAAGTTC ATGCCATGGT CTCCGTATTC 
CAATTTATTA TGAAACAAAG TTACATCTGT GCCCTCATAG CTATGATGGC CTGGAGCATC 
ACCTATCACA GCTGGCTGAC CTTCGTGCTG CTGATCTGGT CGTGCACTCT TTGGATGATT 
CGCAACAGAA GAAAATATGC CATGATCAGC TCTCCCTTCA TGGTGGTTTA TGGAAACCTA 
TTGTTGATAT TACAGTATAT ATGGAGTTTT GAACTTCCTG AAATTAAAAA AGTTCCAGGA 
TTTTTAGAAA AGAAAGAGCC AGGAGAACTT GCTTCCAAAA TCCTTTTCAC CATTACTTTT 
TGGCTACTGC TGAGGCAGCA CCTCACAGAG CAAAAAGCTC TGCAAGAAAA GGAAGCTCTT 
TTATCGGAAG TCAAAATTGG CAGTCAGGAA AATGAAGAAA AAGATGAGGA ACTTCAAGAT 
ATACAAGTGG AAGGAGAGCC CAAAGAGGAG GAAGAAGAGG AAGCGAAGGA AGAGAAGCAA 
GAGAGAAAGA AGGTAGAGCA AGAGGAAGCT GAAGAAGAAG ATGAGCAGGA CATCATGAAA 
GTCCTGGGCA ATCTGGTGGT GGCCATGTTC ATCAAGTACT GGATCTACGT CTGCGGAGGC 
ATGTTCTTCT TCGTCAGCTT CGAGGGTAAA ATCGTAATGT ACAAAATCAT CTACATGGTG 
CTGTTCCTGT TCTGTGTGGC CCTATACCAG GTGCACTATG AATGGTGGAG GAAAATTCTA 
AAATATTTTT GGATGTCAGT GGTTATTTAC ACTATGCTGG TGCTTATCTT TATATACACA 
TATCAGTTTG AGAACTTCCC AGGCCTGTGG CAAAATATGA CTGGACTGAA AAAAGAAAAG 
CTTGAGGATC TTGGCTTAAA GCAGTTTACT GTGGCTGAAC TATTCACTCG CATATTCATC 
CCAACCTCCT TTCTGCTGGT GTGCATTTTA CACCTGCACT ACTTCCATGA CCGGTTCCTT 
GAACTCACAG ACCTCAAGTC CATTCCCAGC AAAGAAGACA ACACCATCTA CAGCCATGCC 
AAAGTCAATG GTCGCGTATA TCTAATAATA AATAGGATCT CACACAGTCT TCCCACGGTT 
GCCAATCGAC AGTTCGCATC AGTAACTCTC AGCATCAAGA AAAAATTACC AATCCACCAA 
AATGAACTGG CCCACCCGGA AGGAAGCCTC CCGGACCTCA CCATGATGCA TCTGACTGCC 
AGCCTGGAGA AGCCGGAGGT GAGGAAGTTG GCTGAGCCTG GGGAGGAGAA GCTTGAGGGC 
TACTCTGAAA AAGCCCAGAA GGGTGATCTT GGGAAAGACA GCGAGGAGTC AGAGGAGGAC 
GGAGAGGAAG AGGAGGAATC CGAGGAGGAG GAAGAAACAT CAGACTTAAG GAACAAATGG 
CACCTGGTGA TTGACCGCCT CACTGTGCTC TTCTTAAAAT TCCTGGAGTA TTTTCACAAG 
CTGCAGGTGT TCATGTGGTG GATTTTGGAG TTGCACATCA TCAAAATCGT TTCCTCTTAC 
ATTATCTGGG TTTCTGTGAA AGAGGTGTCT CTGTTCAACT ATGTATTTTT GATTTCTTGG 
GCTTTTGCTC TGCCGTACGC CAAGCTGCGC CGTCTGGCTT CAAGTGTCTG CACAGTCTGG 
ACGTGTGTGA TCATCGTCTG CAAAATGTTG TACCAGCTCC AAACCATTAA GCCTGAGAAC 
TTCTCTGTTA ACTGTTCCTT GCCAAATGAA AATCAAACAA ACATCCCCTT TAATGAGTTG 
AACAAGTCTC TGCTCTACAG CGCTCCTATC GATCCTACAG AGTGGGTCGG CCTGCGGAAG 
TCTTCGCCTC TGCTAGTCTA CCTGAGGAAT AACCTCCTGA TGCTGGCTAT CCTGGCCTTT 
GAAGTCACCA TTTACCGCCA TCAGGAATAC TATCGAGGTC GAAATAACCT GACGGCCCCT 
GTGTCTAGAA CTATCTTTCA TGACATTACA AGACTACATC TAGATGATGG ACTTATTAAT 
TGTGCCAAAT ATTTCATTAA TTACTTCTTT TACAAGTTTG GTCTGGAGAC CTGTTTCCTA 
ATGTCAGTTA ACGTCATTGG CCAGCGAATG GATTTCTATG CCATGATCCA CGCCTGCTGG 
CTGATCGCTG TCTTATATAG ACGCAGAAGG AAAGCCATCG CAGAGATCTG GCCCAAGTAC 
TGCTGCTTCC TGGCATGCAT CATCACCTTC CAGTATTTCA TCTGCATTGG CATCCCACCT 
GCTCCTTGCC GAGATTACCC GTGGAGATTC AAGGGTGCCA GCTTCAATGA CAACATCATA 
AAGTGGCTGT ACTTCCCAGA TTTCATTGTG CGGCCCAACC CTGTGTTTCT CGTCTATGAC 
TTCATGCTGC TTCTGTGTGC CTCCTTACAA CGGCAGATTT TTGAGGATGA GAACAAGGCT 
GCAGTGCGAA TCATGGCAGG TGACAATGTC GAGATCTGCA TGAACCTTGA TGCGGCCTCC 
TTCAGCCAAC ATAACCCTGT GCCAGATTTT ATTCACTGCA GATCTTACTT AGACATGTCC 
AAAGTGATCA TCTTCAGCTA CCTCTTCTGG TTTGTGCTCA CCATCATCTT CATCACTGGG 
ACCACCAGGA TCAGCATCTT TTGCATGGGG TACCTGGTGG CCTGTTTCTA CTTCCTGCTC 
TTTGGGGGCG ATTTGCTGTT GAAACCCATC AAGAGCATCC TGCGCTACTG GGACTGGCTG 
ATCGCATACA ACGTTTTTGT GATTACGATG AAAAATATCC TGTCAATAGG AGCATGTGGA 
TACATTGGAA CATTGGTGCA CAATAGTTGT TGGTTGATCC AGGCTTTCAG CCTGGCCTGC 
ACAGTCAAAG GCTATCAAAT GCCTGCTGCT AATTCACCCT GTACACTTCC CAGTGGGGAA 
GCAGGAATCA TTTGGGACAG CATATGTTTT GCCTTCCTCC TGCTGCAAAG AAGAGTTTTC 
ATGAGTTATT ATTTTCTACA TGTTGTGGCT GATATAAAAG CTTCCCAGAT TCTGGCATCA 
AGAGGAGCTG AACTTTTCCA GGCCACAATT GTAAAAGCTG TAAAGGCAAG AATTGAGGAA 
GAGAAGAAGT CCATGGACCA GCTGAAGCGA CAGATGGATC GCATCAAGGC CAGGCAACAG 
AAATATAAAA AGGGTAAGGA GAGGATGCTG AGCTTGACCC AGGAGCCAGG GGAAGGCCAG 
GACATGCAAA AACTCTCTGA AGAGGATGAT GAAAGAGAAG CAGACAAACA GAAAGCCAAG 
GGCAAAAAAA AGCAGTGGTG GCGGCCTTGG GTTGATCATG CTTCCATGGT CAGGAGTGGA 
GATTATTATT TGTTTGAAAC GGATAGTGAA GAGGAGGAAG AGGAAGAATT AAAGAAGGAA 
GATGAAGAAC CTCCACGAAG GTCAGCATTC CAGTTTGTTT ATCAAGCCTG GATTACTGAT 
CCTAAAACAG CACTCCGACA AAGACACAAA GAGAAAAAAA GGTCTGCAAG AGAAGAACGG 
AAACGAAGGC GGAAAGGATC CAAGGAGGGT CCTGTGGAAT GGGAAGACCG GGAGGATGAA 
CCAATCAAAA AGAAATCCGA TGGACCAGAT AATATCATCA AGAGGATATT TAATATTTTG 
AAATTTACCT GGGTCCTATT TCTGGCAACA GTGGACAGTT TCACTACTTG GCTTAACTCC 
ATTTCAAGGG AGCATATTGA TATATCTACA GTTCTGAGAA TTGAACGATG CATGCTGACC 
AGAGAAATTA AGAAGGGCAA TGTTCCAACT CGGGAGAGCA TCCACATGTA CTATCAGAAC 
CACATCATGA ACCTTTCCAG AGAGTCGGGA CTGGACACCA TTGACGAGCA TCCCGGAGCT 
GCTTCAGGTG CACAGACAGC CCACAGGATG GATAGTTTAG ATTCACATGA CAGTATCTCC 
AGCGAGCCCA CGCAGTGTAC CATGCTGTAC TCACGCCAGG GGACCACTGA GACCATCGAG 
GAGGTGGAGG CTGAGCAGGA GGAGGAGGCA GGGAGCACGG CGCCTGAGCC CAGGGAGGCC 
AAGGAGTACG AGGCCACTGG GTACGATGTG GGAGCCATGG GTGCCGAGGA GGCCAGCCTC 
ACCCCAGAGG AAGAGCTGAC ACAGTTCTCC ACCTTGGACG GGGATGTGGA GGCCCCACCC 
TCCTACAGCA AGGCTGTGAG CTTCGAGCAT CTGTCCTTCG GCTCGCAGGA CGACTCTGCA 
GGCAAGAACC GTATGGCAGT CAGCCCGGAC GACAGCCGCA CCGACAAGCT GGGGTCCAGC 
ATCTTACCTC CCCTGACCCA TGAGCTGACG GCCAGCGAGC TGCTGCTGAA AAAGATGTTT 
CACGACGATG AGCTTGAAGA GTCAGAGAAA TTCTACGTGG GGCAGCCCCG ATTTCTGCTG 
CTCTTCTATG CCATGTACAA TACCCTGGTG GCCCGCTCGG AGATGGTGTG CTACTTCGTG 
ATCATCCTCA ACCACATGGT CTCTGCCTCC ATGATCACGC TCCTGCTTCC CATCCTCATC 
TTCCTCTGGG CCATGTTGTC CGTCCCCAGG CCCAGCCGCC GGTTCTGGAT GATGGCCATC 
GTCTATACTG AGGTGGCAAT TGTAGTCAAG TATTTCTTCC AATTTGGGTT CTTTCCCTGG 
AATAAGAATG TGGAGGTGAA CAAAGATAAA CCGTATCACC CCCCAAACAT CATAGGAGTG 
GAAAAGAAGG AAGGTTATGT TCTCTATGAC CTCATCCAGC TCCTGGCTCT GTTCTTTCAT 
CGATCAATTT TGAAGTGCCA TGGCTTATGG GATGAAGATG ACATGACTGA AAGTGGCATG 
GCCAGGGAGG AATCAGATGA TGAGCTCTCC CTCGGTCATG GCAGGAGGGA CTCCTCCGAT 
TCTCTCAAGT CCATCAACCT GGCCGCGTCT GTGGAGTCAG TGCATGTGAC CTTCCCGGAG 
CAGCAGACAG CTGTCCGGAG GAAGCGCTCC GGCAGCAGCT CCGAGCCATC CCAGAGATCC 
AGCTTTTCTT CAAACAGATC CCAAAGAGGC AGCACAAGCA CCCGAAACAG CAGTCAAAAA 
GGAAGCAGTG TTTTGAGTAT TAAGCAAAAA GGCAAAAGGG AACTTTATAT GGAAAAGCTT 
CAAGAACATT TAATCAAAGC AAAAGCCTTT ACCATAAAGA AGACGCTGGA GATCTATGTG 
CCCATCAAAC AGTTCTTTTA CAACCTCATC CACCCGGAGT ATAGCGCCGT GACTGACGTG 
TATGTACTCA TGTTCCTGGC TGACACTGTG GACTTCATCA TCATTGTCTT CGGCTTTTGG 
GCCTTTGGGA AACACTCAGC AGCTGCAGAC ATCACCTCTT CACTGTCAGA GGACCAGGTC 
CCGGGGCCGT TTTTGGTGAT GGTCCTCATT CAGTTTGGAA CCATGGTGGT GGACCGAGCC 
CTCTACCTCA GGAAGACTGT ACTGGGAAAG GTCATCTTCC AGGTCATTCT TGTGTTCGGA 
ATTCACTTCT GGATGTTCTT CATCTTACCT GGTGTGACTG AGAGGAAATT CAGCCAGAAC 
CTGGTTGCCC AGCTTTGGTA CTTTGTGAAA TGTGTTTACT TCGGGTTGTC TGCTTACCAG 
ATCCGTTGTG GCTACCCAAC GCGAGTCCTG GGGAACTTCC TCACCAAGAG CTACAATTAC 
GTCAACCTCT TCTTATTCCA AGGGTTTCGC CTCGTGCCCT TTTTGACTGA GCTGAGGGCA 
GTGATGGACT GGGTGTGGAC GGACACAACT TTGAGCCTGT CCAGCTGGAT CTGTGTGGAG 
GACATCTATG CTCACATATT CATCCTGAAG TGTTGGCGGG AGTCGGAGAA GAGATACCCT 
CAGCCACGGG GCCAGAAGAA GAAGAAAGTG GTGAAGTATG GCATGGGAGG AATGATCATC 
GTCCTGCTCA TCTGCATTGT CTGGTTTCCT CTTCTCTTCA TGTCTTTGAT CAAATCTGTG 
GCTGGGGTCA TCAACCAGCC CCTGGACGTC TCCGTCACAA TTACCCTGGG AGGGTATCAG 
CCTATTTTCA CAATGAGTGC CCAACAAAGC CAGTTGAAAG TTATGGACCA GCAGAGCTTT 
AACAAATTTA TACAAGCTTT TTCTAGGGAC ACCGGTGCTA TGCAATTTCT GGAAAATTAT 
GAAAAAGAAG ACATAACAGT AGCAGAACTG GAAGGAAACT CAAATTCTTT GTGGACCATC 
AGCCCACCCA GTAAGCAGAA AATGATACAC GAACTCCTGG ACCCCAATAG TAGCTTCTCT 
GTTGTTTTTT CATGGAGTAT TCAGAGAAAC TTAAGTCTGG GTGCAAAATC GGAAATAGCA 
ACAGATAAGC TTTCTTTTCC TCTTAAAAAT ATTACTCGAA AGAATATCGC TAAAATGATA 
GCAGGCAACA GCACAGAAAG TTCAAAAACA CCAGTGACCA TAGAAAAGAT TTATCCATAT 
TATGTGAAAG CACCTAGTGA TTCTAACTCA AAACCTATAA AGCAACTTTT ATCTGAAAAT 
AATTTCATGG ATATTACCAT CATTTTGTCC AGAGACAATA CAACTAAATA TAACAGTGAG 
TGGTGGGTTC TCAACCTGAC TGGAAACAGA ATATACAATC CGAACTCTCA GGCCCTGGAA 
CTGGTGGTCT TCAATGACAA AGTCAGTCCC CCAAGTCTGG GGTTCCTGGC TGGCTATGGT 
ATTATGGGAT TATATGCTTC AGTTGTCCTT GTGATTGGGA AATTTGTCCG TGAATTCTTC 
AGTGGGATTT CTCACTCCAT CATGTTTGAA GAGCTTCCAA ATGTGGATCG AATTTTGAAG 
TTGTGCACAG ATATTTTTTT AGTTCGAGAG ACAGGAGAAC TGGAGCTAGA AGAAGATCTC 
TATGCCAAAT TAATATTCCT ATATCGCTCA CCAGAGACAA TGATCAAATG GACTAGAGAA 
AAAACAAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881407.1
CDS	973..9324
Translation	MASEVVCGLIFRLLLPICLAVACAFRYNGLSFVYLIYLLLIPLFSEPTKTTMQGHTGRLLKSLCFISLSFLLLHIIFHITLVSLEAQHRIAPGYNCSTWEKTFRQIGFESLKGADAGNGIRVFVPDIGMFIASLTIWLLCRNIVQKPVTDEAAQSNPEFENEELAEGEKIDSEEALIYEEDFNGGDGVEGELEESTKLKMFRRLASVASKLKEFIGNMITTAGKVVVTILLGSSGMMLPSLTSSVYFFVFLGLCTWWSWCRTFDPLLFSCLCVLLAIFTAGHLIGLYLYQFQFFQEAVPPNDYYARLFGIKSVIQTDCSSTWKIIVNPDLSWYHHANPILLLVMYYTLATLIRIWLQEPLVQDEGTKEEDKALACSPIQITAGRRRSLWYATHYPTDERKGLLVTVNGNPVDYHTIHPSLPMENGPGKADLYSTPQYRWEPSDESSEKREEEEEEKEEFEEERSREEKRSIKVHAMVSVFQFIMKQSYICALIAMMAWSITYHSWLTFVLLIWSCTLWMIRNRRKYAMISSPFMVVYGNLLLILQYIWSFELPEIKKVPGFLEKKEPGELASKILFTITFWLLLRQHLTEQKALQEKEALLSEVKIGSQENEEKDEELQDIQVEGEPKEEEEEEAKEEKQERKKVEQEEAEEEDEQDIMKVLGNLVVAMFIKYWIYVCGGMFFFVSFEGKIVMYKIIYMVLFLFCVALYQVHYEWWRKILKYFWMSVVIYTMLVLIFIYTYQFENFPGLWQNMTGLKKEKLEDLGLKQFTVAELFTRIFIPTSFLLVCILHLHYFHDRFLELTDLKSIPSKEDNTIYSHAKVNGRVYLIINRISHSLPTVANRQFASVTLSIKKKLPIHQNELAHPEGSLPDLTMMHLTASLEKPEVRKLAEPGEEKLEGYSEKAQKGDLGKDSEESEEDGEEEEESEEEEETSDLRNKWHLVIDRLTVLFLKFLEYFHKLQVFMWWILELHIIKIVSSYIIWVSVKEVSLFNYVFLISWAFALPYAKLRRLASSVCTVWTCVIIVCKMLYQLQTIKPENFSVNCSLPNENQTNIPFNELNKSLLYSAPIDPTEWVGLRKSSPLLVYLRNNLLMLAILAFEVTIYRHQEYYRGRNNLTAPVSRTIFHDITRLHLDDGLINCAKYFINYFFYKFGLETCFLMSVNVIGQRMDFYAMIHACWLIAVLYRRRRKAIAEIWPKYCCFLACIITFQYFICIGIPPAPCRDYPWRFKGASFNDNIIKWLYFPDFIVRPNPVFLVYDFMLLLCASLQRQIFEDENKAAVRIMAGDNVEICMNLDAASFSQHNPVPDFIHCRSYLDMSKVIIFSYLFWFVLTIIFITGTTRISIFCMGYLVACFYFLLFGGDLLLKPIKSILRYWDWLIAYNVFVITMKNILSIGACGYIGTLVHNSCWLIQAFSLACTVKGYQMPAANSPCTLPSGEAGIIWDSICFAFLLLQRRVFMSYYFLHVVADIKASQILASRGAELFQATIVKAVKARIEEEKKSMDQLKRQMDRIKARQQKYKKGKERMLSLTQEPGEGQDMQKLSEEDDEREADKQKAKGKKKQWWRPWVDHASMVRSGDYYLFETDSEEEEEEELKKEDEEPPRRSAFQFVYQAWITDPKTALRQRHKEKKRSAREERKRRRKGSKEGPVEWEDREDEPIKKKSDGPDNIIKRIFNILKFTWVLFLATVDSFTTWLNSISREHIDISTVLRIERCMLTREIKKGNVPTRESIHMYYQNHIMNLSRESGLDTIDEHPGAASGAQTAHRMDSLDSHDSISSEPTQCTMLYSRQGTTETIEEVEAEQEEEAGSTAPEPREAKEYEATGYDVGAMGAEEASLTPEEELTQFSTLDGDVEAPPSYSKAVSFEHLSFGSQDDSAGKNRMAVSPDDSRTDKLGSSILPPLTHELTASELLLKKMFHDDELEESEKFYVGQPRFLLLFYAMYNTLVARSEMVCYFVIILNHMVSASMITLLLPILIFLWAMLSVPRPSRRFWMMAIVYTEVAIVVKYFFQFGFFPWNKNVEVNKDKPYHPPNIIGVEKKEGYVLYDLIQLLALFFHRSILKCHGLWDEDDMTESGMAREESDDELSLGHGRRDSSDSLKSINLAASVESVHVTFPEQQTAVRRKRSGSSSEPSQRSSFSSNRSQRGSTSTRNSSQKGSSVLSIKQKGKRELYMEKLQEHLIKAKAFTIKKTLEIYVPIKQFFYNLIHPEYSAVTDVYVLMFLADTVDFIIIVFGFWAFGKHSAAADITSSLSEDQVPGPFLVMVLIQFGTMVVDRALYLRKTVLGKVIFQVILVFGIHFWMFFILPGVTERKFSQNLVAQLWYFVKCVYFGLSAYQIRCGYPTRVLGNFLTKSYNYVNLFLFQGFRLVPFLTELRAVMDWVWTDTTLSLSSWICVEDIYAHIFILKCWRESEKRYPQPRGQKKKKVVKYGMGGMIIVLLICIVWFPLLFMSLIKSVAGVINQPLDVSVTITLGGYQPIFTMSAQQSQLKVMDQQSFNKFIQAFSRDTGAMQFLENYEKEDITVAELEGNSNSLWTISPPSKQKMIHELLDPNSSFSVVFSWSIQRNLSLGAKSEIATDKLSFPLKNITRKNIAKMIAGNSTESSKTPVTIEKIYPYYVKAPSDSNSKPIKQLLSENNFMDITIILSRDNTTKYNSEWWVLNLTGNRIYNPNSQALELVVFNDKVSPPSLGFLAGYGIMGLYASVVLVIGKFVREFFSGISHSIMFEELPNVDRILKLCTDIFLVRETGELELEEDLYAKLIFLYRSPETMIKWTREKTN

Target ORF information:

RefSeq Version	XM_017025918.1
Organism	Homo sapiens(human)
Definition	Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025918.1

ORF Insert Sequence:

ATGGCCTCAG AAGTGGTGTG CGGGCTCATC TTCAGGCTGC TGCTGCCCAT CTGCCTGGCA 
GTAGCATGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC 
ATTCCTCTGT TCTCAGAACC AACAAAAACG ACGATGCAAG GACATACGGG ACGGTTATTA 
AAGTCTCTGT GCTTCATCAG TCTTTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG 
TTGGTGAGCC TTGAAGCTCA ACATCGTATT GCACCTGGCT ACAACTGCTC AACATGGGAA 
AAGACATTCC GGCAGATCGG CTTTGAAAGC TTAAAGGGAG CTGATGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCCTCTGT 
AGAAACATTG TTCAGAAACC TGTGACAGAC GAAGCAGCAC AGAGTAACCC GGAGTTTGAA 
AATGAAGAAT TGGCTGAAGG AGAAAAAATT GATTCAGAAG AGGCACTGAT CTATGAAGAG 
GATTTCAATG GAGGAGATGG TGTTGAAGGC GAGTTGGAAG AAAGCACGAA GTTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCCTCTAAG CTCAAGGAGT TCATTGGCAA CATGATCACC 
ACTGCTGGGA AAGTCGTTGT TACCATCTTA CTGGGCTCCT CGGGCATGAT GTTGCCGTCT 
TTGACATCAT CTGTGTATTT TTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACTGCT 
GGACATTTGA TTGGACTTTA TTTATACCAG TTCCAATTCT TTCAAGAGGC AGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAGTCAGTAA TTCAAACGGA CTGTTCAAGT 
ACTTGGAAGA TCATAGTGAA CCCGGACCTG TCGTGGTACC ACCACGCCAA CCCTATCCTC 
CTGCTGGTGA TGTACTACAC TCTGGCCACT CTGATCCGCA TCTGGCTGCA AGAGCCCCTT 
GTGCAGGATG AGGGGACCAA AGAAGAGGAC AAAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACAGCGGGGA GGAGGCGGAG CCTGTGGTAC GCAACCCATT ACCCCACTGA TGAGAGAAAA 
GGCCTGCTGG TGACTGTGAA CGGCAACCCC GTGGATTACC ACACCATCCA CCCAAGCCTG 
CCCATGGAGA ACGGCCCTGG CAAAGCCGAC CTCTACTCCA CCCCTCAGTA CCGGTGGGAG 
CCCTCTGATG AATCCTCAGA AAAGCGAGAG GAGGAAGAGG AAGAGAAAGA AGAATTTGAA 
GAAGAAAGGA GCCGTGAGGA AAAAAGAAGT ATCAAAGTTC ATGCCATGGT CTCCGTATTC 
CAATTTATTA TGAAACAAAG TTACATCTGT GCCCTCATAG CTATGATGGC CTGGAGCATC 
ACCTATCACA GCTGGCTGAC CTTCGTGCTG CTGATCTGGT CGTGCACTCT TTGGATGATT 
CGCAACAGAA GAAAATATGC CATGATCAGC TCTCCCTTCA TGGTGGTTTA TGGAAACCTA 
TTGTTGATAT TACAGTATAT ATGGAGTTTT GAACTTCCTG AAATTAAAAA AGTTCCAGGA 
TTTTTAGAAA AGAAAGAGCC AGGAGAACTT GCTTCCAAAA TCCTTTTCAC CATTACTTTT 
TGGCTACTGC TGAGGCAGCA CCTCACAGAG CAAAAAGCTC TGCAAGAAAA GGAAGCTCTT 
TTATCGGAAG TCAAAATTGG CAGTCAGGAA AATGAAGAAA AAGATGAGGA ACTTCAAGAT 
ATACAAGTGG AAGGAGAGCC CAAAGAGGAG GAAGAAGAGG AAGCGAAGGA AGAGAAGCAA 
GAGAGAAAGA AGGTAGAGCA AGAGGAAGCT GAAGAAGAAG ATGAGCAGGA CATCATGAAA 
GTCCTGGGCA ATCTGGTGGT GGCCATGTTC ATCAAGTACT GGATCTACGT CTGCGGAGGC 
ATGTTCTTCT TCGTCAGCTT CGAGGGTAAA ATCGTAATGT ACAAAATCAT CTACATGGTG 
CTGTTCCTGT TCTGTGTGGC CCTATACCAG GTGCACTATG AATGGTGGAG GAAAATTCTA 
AAATATTTTT GGATGTCAGT GGTTATTTAC ACTATGCTGG TGCTTATCTT TATATACACA 
TATCAGTTTG AGAACTTCCC AGGCCTGTGG CAAAATATGA CTGGACTGAA AAAAGAAAAG 
CTTGAGGATC TTGGCTTAAA GCAGTTTACT GTGGCTGAAC TATTCACTCG CATATTCATC 
CCAACCTCCT TTCTGCTGGT GTGCATTTTA CACCTGCACT ACTTCCATGA CCGGTTCCTT 
GAACTCACAG ACCTCAAGTC CATTCCCAGC AAAGAAGACA ACACCATCTA CAGCCATGCC 
AAAGTCAATG GTCGCGTATA TCTAATAATA AATAGGATCT CACACAGTCT TCCCACGGTT 
GCCAATCGAC AGTTCGCATC AGTAACTCTC AGCATCAAGA AAAAATTACC AATCCACCAA 
AATGAACTGG CCCACCCGGA AGGAAGCCTC CCGGACCTCA CCATGATGCA TCTGACTGCC 
AGCCTGGAGA AGCCGGAGGT GAGGAAGTTG GCTGAGCCTG GGGAGGAGAA GCTTGAGGGC 
TACTCTGAAA AAGCCCAGAA GGGTGATCTT GGGAAAGACA GCGAGGAGTC AGAGGAGGAC 
GGAGAGGAAG AGGAGGAATC CGAGGAGGAG GAAGAAACAT CAGACTTAAG GAACAAATGG 
CACCTGGTGA TTGACCGCCT CACTGTGCTC TTCTTAAAAT TCCTGGAGTA TTTTCACAAG 
CTGCAGGTGT TCATGTGGTG GATTTTGGAG TTGCACATCA TCAAAATCGT TTCCTCTTAC 
ATTATCTGGG TTTCTGTGAA AGAGGTGTCT CTGTTCAACT ATGTATTTTT GATTTCTTGG 
GCTTTTGCTC TGCCGTACGC CAAGCTGCGC CGTCTGGCTT CAAGTGTCTG CACAGTCTGG 
ACGTGTGTGA TCATCGTCTG CAAAATGTTG TACCAGCTCC AAACCATTAA GCCTGAGAAC 
TTCTCTGTTA ACTGTTCCTT GCCAAATGAA AATCAAACAA ACATCCCCTT TAATGAGTTG 
AACAAGTCTC TGCTCTACAG CGCTCCTATC GATCCTACAG AGTGGGTCGG CCTGCGGAAG 
TCTTCGCCTC TGCTAGTCTA CCTGAGGAAT AACCTCCTGA TGCTGGCTAT CCTGGCCTTT 
GAAGTCACCA TTTACCGCCA TCAGGAATAC TATCGAGGTC GAAATAACCT GACGGCCCCT 
GTGTCTAGAA CTATCTTTCA TGACATTACA AGACTACATC TAGATGATGG ACTTATTAAT 
TGTGCCAAAT ATTTCATTAA TTACTTCTTT TACAAGTTTG GTCTGGAGAC CTGTTTCCTA 
ATGTCAGTTA ACGTCATTGG CCAGCGAATG GATTTCTATG CCATGATCCA CGCCTGCTGG 
CTGATCGCTG TCTTATATAG ACGCAGAAGG AAAGCCATCG CAGAGATCTG GCCCAAGTAC 
TGCTGCTTCC TGGCATGCAT CATCACCTTC CAGTATTTCA TCTGCATTGG CATCCCACCT 
GCTCCTTGCC GAGATTACCC GTGGAGATTC AAGGGTGCCA GCTTCAATGA CAACATCATA 
AAGTGGCTGT ACTTCCCAGA TTTCATTGTG CGGCCCAACC CTGTGTTTCT CGTCTATGAC 
TTCATGCTGC TTCTGTGTGC CTCCTTACAA CGGCAGATTT TTGAGGATGA GAACAAGGCT 
GCAGTGCGAA TCATGGCAGG TGACAATGTC GAGATCTGCA TGAACCTTGA TGCGGCCTCC 
TTCAGCCAAC ATAACCCTGT GCCAGATTTT ATTCACTGCA GATCTTACTT AGACATGTCC 
AAAGTGATCA TCTTCAGCTA CCTCTTCTGG TTTGTGCTCA CCATCATCTT CATCACTGGG 
ACCACCAGGA TCAGCATCTT TTGCATGGGG TACCTGGTGG CCTGTTTCTA CTTCCTGCTC 
TTTGGGGGCG ATTTGCTGTT GAAACCCATC AAGAGCATCC TGCGCTACTG GGACTGGCTG 
ATCGCATACA ACGTTTTTGT GATTACGATG AAAAATATCC TGTCAATAGG AGCATGTGGA 
TACATTGGAA CATTGGTGCA CAATAGTTGT TGGTTGATCC AGGCTTTCAG CCTGGCCTGC 
ACAGTCAAAG GCTATCAAAT GCCTGCTGCT AATTCACCCT GTACACTTCC CAGTGGGGAA 
GCAGGAATCA TTTGGGACAG CATATGTTTT GCCTTCCTCC TGCTGCAAAG AAGAGTTTTC 
ATGAGTTATT ATTTTCTACA TGTTGTGGCT GATATAAAAG CTTCCCAGAT TCTGGCATCA 
AGAGGAGCTG AACTTTTCCA GGCCACAATT GTAAAAGCTG TAAAGGCAAG AATTGAGGAA 
GAGAAGAAGT CCATGGACCA GCTGAAGCGA CAGATGGATC GCATCAAGGC CAGGCAACAG 
AAATATAAAA AGGGTAAGGA GAGGATGCTG AGCTTGACCC AGGAGCCAGG GGAAGGCCAG 
GACATGCAAA AACTCTCTGA AGAGGATGAT GAAAGAGAAG CAGACAAACA GAAAGCCAAG 
GGCAAAAAAA AGCAGTGGTG GCGGCCTTGG GTTGATCATG CTTCCATGGT CAGGAGTGGA 
GATTATTATT TGTTTGAAAC GGATAGTGAA GAGGAGGAAG AGGAAGAATT AAAGAAGGAA 
GATGAAGAAC CTCCACGAAG GTCAGCATTC CAGTTTGTTT ATCAAGCCTG GATTACTGAT 
CCTAAAACAG CACTCCGACA AAGACACAAA GAGAAAAAAA GGTCTGCAAG AGAAGAACGG 
AAACGAAGGC GGAAAGGATC CAAGGAGGGT CCTGTGGAAT GGGAAGACCG GGAGGATGAA 
CCAATCAAAA AGAAATCCGA TGGACCAGAT AATATCATCA AGAGGATATT TAATATTTTG 
AAATTTACCT GGGTCCTATT TCTGGCAACA GTGGACAGTT TCACTACTTG GCTTAACTCC 
ATTTCAAGGG AGCATATTGA TATATCTACA GTTCTGAGAA TTGAACGATG CATGCTGACC 
AGAGAAATTA AGAAGGGCAA TGTTCCAACT CGGGAGAGCA TCCACATGTA CTATCAGAAC 
CACATCATGA ACCTTTCCAG AGAGTCGGGA CTGGACACCA TTGACGAGCA TCCCGGAGCT 
GCTTCAGGTG CACAGACAGC CCACAGGATG GATAGTTTAG ATTCACATGA CAGTATCTCC 
AGCGAGCCCA CGCAGTGTAC CATGCTGTAC TCACGCCAGG GGACCACTGA GACCATCGAG 
GAGGTGGAGG CTGAGCAGGA GGAGGAGGCA GGGAGCACGG CGCCTGAGCC CAGGGAGGCC 
AAGGAGTACG AGGCCACTGG GTACGATGTG GGAGCCATGG GTGCCGAGGA GGCCAGCCTC 
ACCCCAGAGG AAGAGCTGAC ACAGTTCTCC ACCTTGGACG GGGATGTGGA GGCCCCACCC 
TCCTACAGCA AGGCTGTGAG CTTCGAGCAT CTGTCCTTCG GCTCGCAGGA CGACTCTGCA 
GGCAAGAACC GTATGGCAGT CAGCCCGGAC GACAGCCGCA CCGACAAGCT GGGGTCCAGC 
ATCTTACCTC CCCTGACCCA TGAGCTGACG GCCAGCGAGC TGCTGCTGAA AAAGATGTTT 
CACGACGATG AGCTTGAAGA GTCAGAGAAA TTCTACGTGG GGCAGCCCCG ATTTCTGCTG 
CTCTTCTATG CCATGTACAA TACCCTGGTG GCCCGCTCGG AGATGGTGTG CTACTTCGTG 
ATCATCCTCA ACCACATGGT CTCTGCCTCC ATGATCACGC TCCTGCTTCC CATCCTCATC 
TTCCTCTGGG CCATGTTGTC CGTCCCCAGG CCCAGCCGCC GGTTCTGGAT GATGGCCATC 
GTCTATACTG AGGTGGCAAT TGTAGTCAAG TATTTCTTCC AATTTGGGTT CTTTCCCTGG 
AATAAGAATG TGGAGGTGAA CAAAGATAAA CCGTATCACC CCCCAAACAT CATAGGAGTG 
GAAAAGAAGG AAGGTTATGT TCTCTATGAC CTCATCCAGC TCCTGGCTCT GTTCTTTCAT 
CGATCAATTT TGAAGTGCCA TGGCTTATGG GATGAAGATG ACATGACTGA AAGTGGCATG 
GCCAGGGAGG AATCAGATGA TGAGCTCTCC CTCGGTCATG GCAGGAGGGA CTCCTCCGAT 
TCTCTCAAGT CCATCAACCT GGCCGCGTCT GTGGAGTCAG TGCATGTGAC CTTCCCGGAG 
CAGCAGACAG CTGTCCGGAG GAAGCGCTCC GGCAGCAGCT CCGAGCCATC CCAGAGATCC 
AGCTTTTCTT CAAACAGATC CCAAAGAGGC AGCACAAGCA CCCGAAACAG CAGTCAAAAA 
GGAAGCAGTG TTTTGAGTAT TAAGCAAAAA GGCAAAAGGG AACTTTATAT GGAAAAGCTT 
CAAGAACATT TAATCAAAGC AAAAGCCTTT ACCATAAAGA AGACGCTGGA GATCTATGTG 
CCCATCAAAC AGTTCTTTTA CAACCTCATC CACCCGGAGT ATAGCGCCGT GACTGACGTG 
TATGTACTCA TGTTCCTGGC TGACACTGTG GACTTCATCA TCATTGTCTT CGGCTTTTGG 
GCCTTTGGGA AACACTCAGC AGCTGCAGAC ATCACCTCTT CACTGTCAGA GGACCAGGTC 
CCGGGGCCGT TTTTGGTGAT GGTCCTCATT CAGTTTGGAA CCATGGTGGT GGACCGAGCC 
CTCTACCTCA GGAAGACTGT ACTGGGAAAG GTCATCTTCC AGGTCATTCT TGTGTTCGGA 
ATTCACTTCT GGATGTTCTT CATCTTACCT GGTGTGACTG AGAGGAAATT CAGCCAGAAC 
CTGGTTGCCC AGCTTTGGTA CTTTGTGAAA TGTGTTTACT TCGGGTTGTC TGCTTACCAG 
ATCCGTTGTG GCTACCCAAC GCGAGTCCTG GGGAACTTCC TCACCAAGAG CTACAATTAC 
GTCAACCTCT TCTTATTCCA AGGGTTTCGC CTCGTGCCCT TTTTGACTGA GCTGAGGGCA 
GTGATGGACT GGGTGTGGAC GGACACAACT TTGAGCCTGT CCAGCTGGAT CTGTGTGGAG 
GACATCTATG CTCACATATT CATCCTGAAG TGTTGGCGGG AGTCGGAGAA GAGATACCCT 
CAGCCACGGG GCCAGAAGAA GAAGAAAGTG GTGAAGTATG GCATGGGAGG AATGATCATC 
GTCCTGCTCA TCTGCATTGT CTGGTTTCCT CTTCTCTTCA TGTCTTTGAT CAAATCTGTG 
GCTGGGGTCA TCAACCAGCC CCTGGACGTC TCCGTCACAA TTACCCTGGG AGGGTATCAG 
CCTATTTTCA CAATGAGTGC CCAACAAAGC CAGTTGAAAG TTATGGACCA GCAGAGCTTT 
AACAAATTTA TACAAGCTTT TTCTAGGGAC ACCGGTGCTA TGCAATTTCT GGAAAATTAT 
GAAAAAGAAG ACATAACAGT AGCAGAACTG GAAGGAAACT CAAATTCTTT GTGGACCATC 
AGCCCACCCA GTAAGCAGAA AATGATACAC GAACTCCTGG ACCCCAATAG TAGCTTCTCT 
GTTGTTTTTT CATGGAGTAT TCAGAGAAAC TTAAGTCTGG GTGCAAAATC GGAAATAGCA 
ACAGATAAGC TTTCTTTTCC TCTTAAAAAT ATTACTCGAA AGAATATCGC TAAAATGATA 
GCAGGCAACA GCACAGAAAG TTCAAAAACA CCAGTGACCA TAGAAAAGAT TTATCCATAT 
TATGTGAAAG CACCTAGTGA TTCTAACTCA AAACCTATAA AGCAACTTTT ATCTGAAAAT 
AATTTCATGG ATATTACCAT CATTTTGTCC AGAGACAATA CAACTAAATA TAACAGTGAG 
TGGTGGGTTC TCAACCTGAC TGGAAACAGA ATATACAATC CGAACTCTCA GGCCCTGGAA 
CTGGTGGTCT TCAATGACAA AGTCAGTCCC CCAAGTCTGG GGTTCCTGGC TGGCTATGGT 
ATTATGGGAT TATATGCTTC AGTTGTCCTT GTGATTGGGA AATTTGTCCG TGAATTCTTC 
AGTGGGATTT CTCACTCCAT CATGTTTGAA GAGCTTCCAA ATGTGGATCG AATTTTGAAG 
TTGTGCACAG ATATTTTTTT AGTTCGAGAG ACAGGAGAAC TGGAGCTAGA AGAAGATCTC 
TATGCCAAAT TAATATTCCT ATATCGCTCA CCAGAGACAA TGATCAAATG GACTAGAGAA 
AAAACAAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIEZO2 ( XM_017025918.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881407.1 Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X2, mRNA.

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CloneID	OHu88005
Clone ID Related Accession (Same CDS sequence)	XM_017025918.1 , XM_017025918.2
Accession Version	XM_017025918.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	piezo-type mechanosensitive ion channel component 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017025918.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG AAGTGGTGTG CGGGCTCATC TTCAGGCTGC TGCTGCCCAT CTGCCTGGCA 
GTAGCATGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC 
ATTCCTCTGT TCTCAGAACC AACAAAAACG ACGATGCAAG GACATACGGG ACGGTTATTA 
AAGTCTCTGT GCTTCATCAG TCTTTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG 
TTGGTGAGCC TTGAAGCTCA ACATCGTATT GCACCTGGCT ACAACTGCTC AACATGGGAA 
AAGACATTCC GGCAGATCGG CTTTGAAAGC TTAAAGGGAG CTGATGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCCTCTGT 
AGAAACATTG TTCAGAAACC TGTGACAGAC GAAGCAGCAC AGAGTAACCC GGAGTTTGAA 
AATGAAGAAT TGGCTGAAGG AGAAAAAATT GATTCAGAAG AGGCACTGAT CTATGAAGAG 
GATTTCAATG GAGGAGATGG TGTTGAAGGC GAGTTGGAAG AAAGCACGAA GTTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCCTCTAAG CTCAAGGAGT TCATTGGCAA CATGATCACC 
ACTGCTGGGA AAGTCGTTGT TACCATCTTA CTGGGCTCCT CGGGCATGAT GTTGCCGTCT 
TTGACATCAT CTGTGTATTT TTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACTGCT 
GGACATTTGA TTGGACTTTA TTTATACCAG TTCCAATTCT TTCAAGAGGC AGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAGTCAGTAA TTCAAACGGA CTGTTCAAGT 
ACTTGGAAGA TCATAGTGAA CCCGGACCTG TCGTGGTACC ACCACGCCAA CCCTATCCTC 
CTGCTGGTGA TGTACTACAC TCTGGCCACT CTGATCCGCA TCTGGCTGCA AGAGCCCCTT 
GTGCAGGATG AGGGGACCAA AGAAGAGGAC AAAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACAGCGGGGA GGAGGCGGAG CCTGTGGTAC GCAACCCATT ACCCCACTGA TGAGAGAAAA 
GGCCTGCTGG TGACTGTGAA CGGCAACCCC GTGGATTACC ACACCATCCA CCCAAGCCTG 
CCCATGGAGA ACGGCCCTGG CAAAGCCGAC CTCTACTCCA CCCCTCAGTA CCGGTGGGAG 
CCCTCTGATG AATCCTCAGA AAAGCGAGAG GAGGAAGAGG AAGAGAAAGA AGAATTTGAA 
GAAGAAAGGA GCCGTGAGGA AAAAAGAAGT ATCAAAGTTC ATGCCATGGT CTCCGTATTC 
CAATTTATTA TGAAACAAAG TTACATCTGT GCCCTCATAG CTATGATGGC CTGGAGCATC 
ACCTATCACA GCTGGCTGAC CTTCGTGCTG CTGATCTGGT CGTGCACTCT TTGGATGATT 
CGCAACAGAA GAAAATATGC CATGATCAGC TCTCCCTTCA TGGTGGTTTA TGGAAACCTA 
TTGTTGATAT TACAGTATAT ATGGAGTTTT GAACTTCCTG AAATTAAAAA AGTTCCAGGA 
TTTTTAGAAA AGAAAGAGCC AGGAGAACTT GCTTCCAAAA TCCTTTTCAC CATTACTTTT 
TGGCTACTGC TGAGGCAGCA CCTCACAGAG CAAAAAGCTC TGCAAGAAAA GGAAGCTCTT 
TTATCGGAAG TCAAAATTGG CAGTCAGGAA AATGAAGAAA AAGATGAGGA ACTTCAAGAT 
ATACAAGTGG AAGGAGAGCC CAAAGAGGAG GAAGAAGAGG AAGCGAAGGA AGAGAAGCAA 
GAGAGAAAGA AGGTAGAGCA AGAGGAAGCT GAAGAAGAAG ATGAGCAGGA CATCATGAAA 
GTCCTGGGCA ATCTGGTGGT GGCCATGTTC ATCAAGTACT GGATCTACGT CTGCGGAGGC 
ATGTTCTTCT TCGTCAGCTT CGAGGGTAAA ATCGTAATGT ACAAAATCAT CTACATGGTG 
CTGTTCCTGT TCTGTGTGGC CCTATACCAG GTGCACTATG AATGGTGGAG GAAAATTCTA 
AAATATTTTT GGATGTCAGT GGTTATTTAC ACTATGCTGG TGCTTATCTT TATATACACA 
TATCAGTTTG AGAACTTCCC AGGCCTGTGG CAAAATATGA CTGGACTGAA AAAAGAAAAG 
CTTGAGGATC TTGGCTTAAA GCAGTTTACT GTGGCTGAAC TATTCACTCG CATATTCATC 
CCAACCTCCT TTCTGCTGGT GTGCATTTTA CACCTGCACT ACTTCCATGA CCGGTTCCTT 
GAACTCACAG ACCTCAAGTC CATTCCCAGC AAAGAAGACA ACACCATCTA CAGCCATGCC 
AAAGTCAATG GTCGCGTATA TCTAATAATA AATAGGATCT CACACAGTCT TCCCACGGTT 
GCCAATCGAC AGTTCGCATC AGTAACTCTC AGCATCAAGA AAAAATTACC AATCCACCAA 
AATGAACTGG CCCACCCGGA AGGAAGCCTC CCGGACCTCA CCATGATGCA TCTGACTGCC 
AGCCTGGAGA AGCCGGAGGT GAGGAAGTTG GCTGAGCCTG GGGAGGAGAA GCTTGAGGGC 
TACTCTGAAA AAGCCCAGAA GGGTGATCTT GGGAAAGACA GCGAGGAGTC AGAGGAGGAC 
GGAGAGGAAG AGGAGGAATC CGAGGAGGAG GAAGAAACAT CAGACTTAAG GAACAAATGG 
CACCTGGTGA TTGACCGCCT CACTGTGCTC TTCTTAAAAT TCCTGGAGTA TTTTCACAAG 
CTGCAGGTGT TCATGTGGTG GATTTTGGAG TTGCACATCA TCAAAATCGT TTCCTCTTAC 
ATTATCTGGG TTTCTGTGAA AGAGGTGTCT CTGTTCAACT ATGTATTTTT GATTTCTTGG 
GCTTTTGCTC TGCCGTACGC CAAGCTGCGC CGTCTGGCTT CAAGTGTCTG CACAGTCTGG 
ACGTGTGTGA TCATCGTCTG CAAAATGTTG TACCAGCTCC AAACCATTAA GCCTGAGAAC 
TTCTCTGTTA ACTGTTCCTT GCCAAATGAA AATCAAACAA ACATCCCCTT TAATGAGTTG 
AACAAGTCTC TGCTCTACAG CGCTCCTATC GATCCTACAG AGTGGGTCGG CCTGCGGAAG 
TCTTCGCCTC TGCTAGTCTA CCTGAGGAAT AACCTCCTGA TGCTGGCTAT CCTGGCCTTT 
GAAGTCACCA TTTACCGCCA TCAGGAATAC TATCGAGGTC GAAATAACCT GACGGCCCCT 
GTGTCTAGAA CTATCTTTCA TGACATTACA AGACTACATC TAGATGATGG ACTTATTAAT 
TGTGCCAAAT ATTTCATTAA TTACTTCTTT TACAAGTTTG GTCTGGAGAC CTGTTTCCTA 
ATGTCAGTTA ACGTCATTGG CCAGCGAATG GATTTCTATG CCATGATCCA CGCCTGCTGG 
CTGATCGCTG TCTTATATAG ACGCAGAAGG AAAGCCATCG CAGAGATCTG GCCCAAGTAC 
TGCTGCTTCC TGGCATGCAT CATCACCTTC CAGTATTTCA TCTGCATTGG CATCCCACCT 
GCTCCTTGCC GAGATTACCC GTGGAGATTC AAGGGTGCCA GCTTCAATGA CAACATCATA 
AAGTGGCTGT ACTTCCCAGA TTTCATTGTG CGGCCCAACC CTGTGTTTCT CGTCTATGAC 
TTCATGCTGC TTCTGTGTGC CTCCTTACAA CGGCAGATTT TTGAGGATGA GAACAAGGCT 
GCAGTGCGAA TCATGGCAGG TGACAATGTC GAGATCTGCA TGAACCTTGA TGCGGCCTCC 
TTCAGCCAAC ATAACCCTGT GCCAGATTTT ATTCACTGCA GATCTTACTT AGACATGTCC 
AAAGTGATCA TCTTCAGCTA CCTCTTCTGG TTTGTGCTCA CCATCATCTT CATCACTGGG 
ACCACCAGGA TCAGCATCTT TTGCATGGGG TACCTGGTGG CCTGTTTCTA CTTCCTGCTC 
TTTGGGGGCG ATTTGCTGTT GAAACCCATC AAGAGCATCC TGCGCTACTG GGACTGGCTG 
ATCGCATACA ACGTTTTTGT GATTACGATG AAAAATATCC TGTCAATAGG AGCATGTGGA 
TACATTGGAA CATTGGTGCA CAATAGTTGT TGGTTGATCC AGGCTTTCAG CCTGGCCTGC 
ACAGTCAAAG GCTATCAAAT GCCTGCTGCT AATTCACCCT GTACACTTCC CAGTGGGGAA 
GCAGGAATCA TTTGGGACAG CATATGTTTT GCCTTCCTCC TGCTGCAAAG AAGAGTTTTC 
ATGAGTTATT ATTTTCTACA TGTTGTGGCT GATATAAAAG CTTCCCAGAT TCTGGCATCA 
AGAGGAGCTG AACTTTTCCA GGCCACAATT GTAAAAGCTG TAAAGGCAAG AATTGAGGAA 
GAGAAGAAGT CCATGGACCA GCTGAAGCGA CAGATGGATC GCATCAAGGC CAGGCAACAG 
AAATATAAAA AGGGTAAGGA GAGGATGCTG AGCTTGACCC AGGAGCCAGG GGAAGGCCAG 
GACATGCAAA AACTCTCTGA AGAGGATGAT GAAAGAGAAG CAGACAAACA GAAAGCCAAG 
GGCAAAAAAA AGCAGTGGTG GCGGCCTTGG GTTGATCATG CTTCCATGGT CAGGAGTGGA 
GATTATTATT TGTTTGAAAC GGATAGTGAA GAGGAGGAAG AGGAAGAATT AAAGAAGGAA 
GATGAAGAAC CTCCACGAAG GTCAGCATTC CAGTTTGTTT ATCAAGCCTG GATTACTGAT 
CCTAAAACAG CACTCCGACA AAGACACAAA GAGAAAAAAA GGTCTGCAAG AGAAGAACGG 
AAACGAAGGC GGAAAGGATC CAAGGAGGGT CCTGTGGAAT GGGAAGACCG GGAGGATGAA 
CCAATCAAAA AGAAATCCGA TGGACCAGAT AATATCATCA AGAGGATATT TAATATTTTG 
AAATTTACCT GGGTCCTATT TCTGGCAACA GTGGACAGTT TCACTACTTG GCTTAACTCC 
ATTTCAAGGG AGCATATTGA TATATCTACA GTTCTGAGAA TTGAACGATG CATGCTGACC 
AGAGAAATTA AGAAGGGCAA TGTTCCAACT CGGGAGAGCA TCCACATGTA CTATCAGAAC 
CACATCATGA ACCTTTCCAG AGAGTCGGGA CTGGACACCA TTGACGAGCA TCCCGGAGCT 
GCTTCAGGTG CACAGACAGC CCACAGGATG GATAGTTTAG ATTCACATGA CAGTATCTCC 
AGCGAGCCCA CGCAGTGTAC CATGCTGTAC TCACGCCAGG GGACCACTGA GACCATCGAG 
GAGGTGGAGG CTGAGCAGGA GGAGGAGGCA GGGAGCACGG CGCCTGAGCC CAGGGAGGCC 
AAGGAGTACG AGGCCACTGG GTACGATGTG GGAGCCATGG GTGCCGAGGA GGCCAGCCTC 
ACCCCAGAGG AAGAGCTGAC ACAGTTCTCC ACCTTGGACG GGGATGTGGA GGCCCCACCC 
TCCTACAGCA AGGCTGTGAG CTTCGAGCAT CTGTCCTTCG GCTCGCAGGA CGACTCTGCA 
GGCAAGAACC GTATGGCAGT CAGCCCGGAC GACAGCCGCA CCGACAAGCT GGGGTCCAGC 
ATCTTACCTC CCCTGACCCA TGAGCTGACG GCCAGCGAGC TGCTGCTGAA AAAGATGTTT 
CACGACGATG AGCTTGAAGA GTCAGAGAAA TTCTACGTGG GGCAGCCCCG ATTTCTGCTG 
CTCTTCTATG CCATGTACAA TACCCTGGTG GCCCGCTCGG AGATGGTGTG CTACTTCGTG 
ATCATCCTCA ACCACATGGT CTCTGCCTCC ATGATCACGC TCCTGCTTCC CATCCTCATC 
TTCCTCTGGG CCATGTTGTC CGTCCCCAGG CCCAGCCGCC GGTTCTGGAT GATGGCCATC 
GTCTATACTG AGGTGGCAAT TGTAGTCAAG TATTTCTTCC AATTTGGGTT CTTTCCCTGG 
AATAAGAATG TGGAGGTGAA CAAAGATAAA CCGTATCACC CCCCAAACAT CATAGGAGTG 
GAAAAGAAGG AAGGTTATGT TCTCTATGAC CTCATCCAGC TCCTGGCTCT GTTCTTTCAT 
CGATCAATTT TGAAGTGCCA TGGCTTATGG GATGAAGATG ACATGACTGA AAGTGGCATG 
GCCAGGGAGG AATCAGATGA TGAGCTCTCC CTCGGTCATG GCAGGAGGGA CTCCTCCGAT 
TCTCTCAAGT CCATCAACCT GGCCGCGTCT GTGGAGTCAG TGCATGTGAC CTTCCCGGAG 
CAGCAGACAG CTGTCCGGAG GAAGCGCTCC GGCAGCAGCT CCGAGCCATC CCAGAGATCC 
AGCTTTTCTT CAAACAGATC CCAAAGAGGC AGCACAAGCA CCCGAAACAG CAGTCAAAAA 
GGAAGCAGTG TTTTGAGTAT TAAGCAAAAA GGCAAAAGGG AACTTTATAT GGAAAAGCTT 
CAAGAACATT TAATCAAAGC AAAAGCCTTT ACCATAAAGA AGACGCTGGA GATCTATGTG 
CCCATCAAAC AGTTCTTTTA CAACCTCATC CACCCGGAGT ATAGCGCCGT GACTGACGTG 
TATGTACTCA TGTTCCTGGC TGACACTGTG GACTTCATCA TCATTGTCTT CGGCTTTTGG 
GCCTTTGGGA AACACTCAGC AGCTGCAGAC ATCACCTCTT CACTGTCAGA GGACCAGGTC 
CCGGGGCCGT TTTTGGTGAT GGTCCTCATT CAGTTTGGAA CCATGGTGGT GGACCGAGCC 
CTCTACCTCA GGAAGACTGT ACTGGGAAAG GTCATCTTCC AGGTCATTCT TGTGTTCGGA 
ATTCACTTCT GGATGTTCTT CATCTTACCT GGTGTGACTG AGAGGAAATT CAGCCAGAAC 
CTGGTTGCCC AGCTTTGGTA CTTTGTGAAA TGTGTTTACT TCGGGTTGTC TGCTTACCAG 
ATCCGTTGTG GCTACCCAAC GCGAGTCCTG GGGAACTTCC TCACCAAGAG CTACAATTAC 
GTCAACCTCT TCTTATTCCA AGGGTTTCGC CTCGTGCCCT TTTTGACTGA GCTGAGGGCA 
GTGATGGACT GGGTGTGGAC GGACACAACT TTGAGCCTGT CCAGCTGGAT CTGTGTGGAG 
GACATCTATG CTCACATATT CATCCTGAAG TGTTGGCGGG AGTCGGAGAA GAGATACCCT 
CAGCCACGGG GCCAGAAGAA GAAGAAAGTG GTGAAGTATG GCATGGGAGG AATGATCATC 
GTCCTGCTCA TCTGCATTGT CTGGTTTCCT CTTCTCTTCA TGTCTTTGAT CAAATCTGTG 
GCTGGGGTCA TCAACCAGCC CCTGGACGTC TCCGTCACAA TTACCCTGGG AGGGTATCAG 
CCTATTTTCA CAATGAGTGC CCAACAAAGC CAGTTGAAAG TTATGGACCA GCAGAGCTTT 
AACAAATTTA TACAAGCTTT TTCTAGGGAC ACCGGTGCTA TGCAATTTCT GGAAAATTAT 
GAAAAAGAAG ACATAACAGT AGCAGAACTG GAAGGAAACT CAAATTCTTT GTGGACCATC 
AGCCCACCCA GTAAGCAGAA AATGATACAC GAACTCCTGG ACCCCAATAG TAGCTTCTCT 
GTTGTTTTTT CATGGAGTAT TCAGAGAAAC TTAAGTCTGG GTGCAAAATC GGAAATAGCA 
ACAGATAAGC TTTCTTTTCC TCTTAAAAAT ATTACTCGAA AGAATATCGC TAAAATGATA 
GCAGGCAACA GCACAGAAAG TTCAAAAACA CCAGTGACCA TAGAAAAGAT TTATCCATAT 
TATGTGAAAG CACCTAGTGA TTCTAACTCA AAACCTATAA AGCAACTTTT ATCTGAAAAT 
AATTTCATGG ATATTACCAT CATTTTGTCC AGAGACAATA CAACTAAATA TAACAGTGAG 
TGGTGGGTTC TCAACCTGAC TGGAAACAGA ATATACAATC CGAACTCTCA GGCCCTGGAA 
CTGGTGGTCT TCAATGACAA AGTCAGTCCC CCAAGTCTGG GGTTCCTGGC TGGCTATGGT 
ATTATGGGAT TATATGCTTC AGTTGTCCTT GTGATTGGGA AATTTGTCCG TGAATTCTTC 
AGTGGGATTT CTCACTCCAT CATGTTTGAA GAGCTTCCAA ATGTGGATCG AATTTTGAAG 
TTGTGCACAG ATATTTTTTT AGTTCGAGAG ACAGGAGAAC TGGAGCTAGA AGAAGATCTC 
TATGCCAAAT TAATATTCCT ATATCGCTCA CCAGAGACAA TGATCAAATG GACTAGAGAA 
AAAACAAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881407.1
CDS	998..9349
Translation	MASEVVCGLIFRLLLPICLAVACAFRYNGLSFVYLIYLLLIPLFSEPTKTTMQGHTGRLLKSLCFISLSFLLLHIIFHITLVSLEAQHRIAPGYNCSTWEKTFRQIGFESLKGADAGNGIRVFVPDIGMFIASLTIWLLCRNIVQKPVTDEAAQSNPEFENEELAEGEKIDSEEALIYEEDFNGGDGVEGELEESTKLKMFRRLASVASKLKEFIGNMITTAGKVVVTILLGSSGMMLPSLTSSVYFFVFLGLCTWWSWCRTFDPLLFSCLCVLLAIFTAGHLIGLYLYQFQFFQEAVPPNDYYARLFGIKSVIQTDCSSTWKIIVNPDLSWYHHANPILLLVMYYTLATLIRIWLQEPLVQDEGTKEEDKALACSPIQITAGRRRSLWYATHYPTDERKGLLVTVNGNPVDYHTIHPSLPMENGPGKADLYSTPQYRWEPSDESSEKREEEEEEKEEFEEERSREEKRSIKVHAMVSVFQFIMKQSYICALIAMMAWSITYHSWLTFVLLIWSCTLWMIRNRRKYAMISSPFMVVYGNLLLILQYIWSFELPEIKKVPGFLEKKEPGELASKILFTITFWLLLRQHLTEQKALQEKEALLSEVKIGSQENEEKDEELQDIQVEGEPKEEEEEEAKEEKQERKKVEQEEAEEEDEQDIMKVLGNLVVAMFIKYWIYVCGGMFFFVSFEGKIVMYKIIYMVLFLFCVALYQVHYEWWRKILKYFWMSVVIYTMLVLIFIYTYQFENFPGLWQNMTGLKKEKLEDLGLKQFTVAELFTRIFIPTSFLLVCILHLHYFHDRFLELTDLKSIPSKEDNTIYSHAKVNGRVYLIINRISHSLPTVANRQFASVTLSIKKKLPIHQNELAHPEGSLPDLTMMHLTASLEKPEVRKLAEPGEEKLEGYSEKAQKGDLGKDSEESEEDGEEEEESEEEEETSDLRNKWHLVIDRLTVLFLKFLEYFHKLQVFMWWILELHIIKIVSSYIIWVSVKEVSLFNYVFLISWAFALPYAKLRRLASSVCTVWTCVIIVCKMLYQLQTIKPENFSVNCSLPNENQTNIPFNELNKSLLYSAPIDPTEWVGLRKSSPLLVYLRNNLLMLAILAFEVTIYRHQEYYRGRNNLTAPVSRTIFHDITRLHLDDGLINCAKYFINYFFYKFGLETCFLMSVNVIGQRMDFYAMIHACWLIAVLYRRRRKAIAEIWPKYCCFLACIITFQYFICIGIPPAPCRDYPWRFKGASFNDNIIKWLYFPDFIVRPNPVFLVYDFMLLLCASLQRQIFEDENKAAVRIMAGDNVEICMNLDAASFSQHNPVPDFIHCRSYLDMSKVIIFSYLFWFVLTIIFITGTTRISIFCMGYLVACFYFLLFGGDLLLKPIKSILRYWDWLIAYNVFVITMKNILSIGACGYIGTLVHNSCWLIQAFSLACTVKGYQMPAANSPCTLPSGEAGIIWDSICFAFLLLQRRVFMSYYFLHVVADIKASQILASRGAELFQATIVKAVKARIEEEKKSMDQLKRQMDRIKARQQKYKKGKERMLSLTQEPGEGQDMQKLSEEDDEREADKQKAKGKKKQWWRPWVDHASMVRSGDYYLFETDSEEEEEEELKKEDEEPPRRSAFQFVYQAWITDPKTALRQRHKEKKRSAREERKRRRKGSKEGPVEWEDREDEPIKKKSDGPDNIIKRIFNILKFTWVLFLATVDSFTTWLNSISREHIDISTVLRIERCMLTREIKKGNVPTRESIHMYYQNHIMNLSRESGLDTIDEHPGAASGAQTAHRMDSLDSHDSISSEPTQCTMLYSRQGTTETIEEVEAEQEEEAGSTAPEPREAKEYEATGYDVGAMGAEEASLTPEEELTQFSTLDGDVEAPPSYSKAVSFEHLSFGSQDDSAGKNRMAVSPDDSRTDKLGSSILPPLTHELTASELLLKKMFHDDELEESEKFYVGQPRFLLLFYAMYNTLVARSEMVCYFVIILNHMVSASMITLLLPILIFLWAMLSVPRPSRRFWMMAIVYTEVAIVVKYFFQFGFFPWNKNVEVNKDKPYHPPNIIGVEKKEGYVLYDLIQLLALFFHRSILKCHGLWDEDDMTESGMAREESDDELSLGHGRRDSSDSLKSINLAASVESVHVTFPEQQTAVRRKRSGSSSEPSQRSSFSSNRSQRGSTSTRNSSQKGSSVLSIKQKGKRELYMEKLQEHLIKAKAFTIKKTLEIYVPIKQFFYNLIHPEYSAVTDVYVLMFLADTVDFIIIVFGFWAFGKHSAAADITSSLSEDQVPGPFLVMVLIQFGTMVVDRALYLRKTVLGKVIFQVILVFGIHFWMFFILPGVTERKFSQNLVAQLWYFVKCVYFGLSAYQIRCGYPTRVLGNFLTKSYNYVNLFLFQGFRLVPFLTELRAVMDWVWTDTTLSLSSWICVEDIYAHIFILKCWRESEKRYPQPRGQKKKKVVKYGMGGMIIVLLICIVWFPLLFMSLIKSVAGVINQPLDVSVTITLGGYQPIFTMSAQQSQLKVMDQQSFNKFIQAFSRDTGAMQFLENYEKEDITVAELEGNSNSLWTISPPSKQKMIHELLDPNSSFSVVFSWSIQRNLSLGAKSEIATDKLSFPLKNITRKNIAKMIAGNSTESSKTPVTIEKIYPYYVKAPSDSNSKPIKQLLSENNFMDITIILSRDNTTKYNSEWWVLNLTGNRIYNPNSQALELVVFNDKVSPPSLGFLAGYGIMGLYASVVLVIGKFVREFFSGISHSIMFEELPNVDRILKLCTDIFLVRETGELELEEDLYAKLIFLYRSPETMIKWTREKTN

Target ORF information:

RefSeq Version	XM_017025918.2
Organism	Homo sapiens(human)
Definition	Homo sapiens piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025918.2

ORF Insert Sequence:

ATGGCCTCAG AAGTGGTGTG CGGGCTCATC TTCAGGCTGC TGCTGCCCAT CTGCCTGGCA 
GTAGCATGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTTATCTA CCTCTTGCTC 
ATTCCTCTGT TCTCAGAACC AACAAAAACG ACGATGCAAG GACATACGGG ACGGTTATTA 
AAGTCTCTGT GCTTCATCAG TCTTTCCTTC CTGTTGCTGC ACATCATTTT CCACATCACG 
TTGGTGAGCC TTGAAGCTCA ACATCGTATT GCACCTGGCT ACAACTGCTC AACATGGGAA 
AAGACATTCC GGCAGATCGG CTTTGAAAGC TTAAAGGGAG CTGATGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCCTCTGT 
AGAAACATTG TTCAGAAACC TGTGACAGAC GAAGCAGCAC AGAGTAACCC GGAGTTTGAA 
AATGAAGAAT TGGCTGAAGG AGAAAAAATT GATTCAGAAG AGGCACTGAT CTATGAAGAG 
GATTTCAATG GAGGAGATGG TGTTGAAGGC GAGTTGGAAG AAAGCACGAA GTTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCCTCTAAG CTCAAGGAGT TCATTGGCAA CATGATCACC 
ACTGCTGGGA AAGTCGTTGT TACCATCTTA CTGGGCTCCT CGGGCATGAT GTTGCCGTCT 
TTGACATCAT CTGTGTATTT TTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACTGCT 
GGACATTTGA TTGGACTTTA TTTATACCAG TTCCAATTCT TTCAAGAGGC AGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAGTCAGTAA TTCAAACGGA CTGTTCAAGT 
ACTTGGAAGA TCATAGTGAA CCCGGACCTG TCGTGGTACC ACCACGCCAA CCCTATCCTC 
CTGCTGGTGA TGTACTACAC TCTGGCCACT CTGATCCGCA TCTGGCTGCA AGAGCCCCTT 
GTGCAGGATG AGGGGACCAA AGAAGAGGAC AAAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACAGCGGGGA GGAGGCGGAG CCTGTGGTAC GCAACCCATT ACCCCACTGA TGAGAGAAAA 
GGCCTGCTGG TGACTGTGAA CGGCAACCCC GTGGATTACC ACACCATCCA CCCAAGCCTG 
CCCATGGAGA ACGGCCCTGG CAAAGCCGAC CTCTACTCCA CCCCTCAGTA CCGGTGGGAG 
CCCTCTGATG AATCCTCAGA AAAGCGAGAG GAGGAAGAGG AAGAGAAAGA AGAATTTGAA 
GAAGAAAGGA GCCGTGAGGA AAAAAGAAGT ATCAAAGTTC ATGCCATGGT CTCCGTATTC 
CAATTTATTA TGAAACAAAG TTACATCTGT GCCCTCATAG CTATGATGGC CTGGAGCATC 
ACCTATCACA GCTGGCTGAC CTTCGTGCTG CTGATCTGGT CGTGCACTCT TTGGATGATT 
CGCAACAGAA GAAAATATGC CATGATCAGC TCTCCCTTCA TGGTGGTTTA TGGAAACCTA 
TTGTTGATAT TACAGTATAT ATGGAGTTTT GAACTTCCTG AAATTAAAAA AGTTCCAGGA 
TTTTTAGAAA AGAAAGAGCC AGGAGAACTT GCTTCCAAAA TCCTTTTCAC CATTACTTTT 
TGGCTACTGC TGAGGCAGCA CCTCACAGAG CAAAAAGCTC TGCAAGAAAA GGAAGCTCTT 
TTATCGGAAG TCAAAATTGG CAGTCAGGAA AATGAAGAAA AAGATGAGGA ACTTCAAGAT 
ATACAAGTGG AAGGAGAGCC CAAAGAGGAG GAAGAAGAGG AAGCGAAGGA AGAGAAGCAA 
GAGAGAAAGA AGGTAGAGCA AGAGGAAGCT GAAGAAGAAG ATGAGCAGGA CATCATGAAA 
GTCCTGGGCA ATCTGGTGGT GGCCATGTTC ATCAAGTACT GGATCTACGT CTGCGGAGGC 
ATGTTCTTCT TCGTCAGCTT CGAGGGTAAA ATCGTAATGT ACAAAATCAT CTACATGGTG 
CTGTTCCTGT TCTGTGTGGC CCTATACCAG GTGCACTATG AATGGTGGAG GAAAATTCTA 
AAATATTTTT GGATGTCAGT GGTTATTTAC ACTATGCTGG TGCTTATCTT TATATACACA 
TATCAGTTTG AGAACTTCCC AGGCCTGTGG CAAAATATGA CTGGACTGAA AAAAGAAAAG 
CTTGAGGATC TTGGCTTAAA GCAGTTTACT GTGGCTGAAC TATTCACTCG CATATTCATC 
CCAACCTCCT TTCTGCTGGT GTGCATTTTA CACCTGCACT ACTTCCATGA CCGGTTCCTT 
GAACTCACAG ACCTCAAGTC CATTCCCAGC AAAGAAGACA ACACCATCTA CAGCCATGCC 
AAAGTCAATG GTCGCGTATA TCTAATAATA AATAGGATCT CACACAGTCT TCCCACGGTT 
GCCAATCGAC AGTTCGCATC AGTAACTCTC AGCATCAAGA AAAAATTACC AATCCACCAA 
AATGAACTGG CCCACCCGGA AGGAAGCCTC CCGGACCTCA CCATGATGCA TCTGACTGCC 
AGCCTGGAGA AGCCGGAGGT GAGGAAGTTG GCTGAGCCTG GGGAGGAGAA GCTTGAGGGC 
TACTCTGAAA AAGCCCAGAA GGGTGATCTT GGGAAAGACA GCGAGGAGTC AGAGGAGGAC 
GGAGAGGAAG AGGAGGAATC CGAGGAGGAG GAAGAAACAT CAGACTTAAG GAACAAATGG 
CACCTGGTGA TTGACCGCCT CACTGTGCTC TTCTTAAAAT TCCTGGAGTA TTTTCACAAG 
CTGCAGGTGT TCATGTGGTG GATTTTGGAG TTGCACATCA TCAAAATCGT TTCCTCTTAC 
ATTATCTGGG TTTCTGTGAA AGAGGTGTCT CTGTTCAACT ATGTATTTTT GATTTCTTGG 
GCTTTTGCTC TGCCGTACGC CAAGCTGCGC CGTCTGGCTT CAAGTGTCTG CACAGTCTGG 
ACGTGTGTGA TCATCGTCTG CAAAATGTTG TACCAGCTCC AAACCATTAA GCCTGAGAAC 
TTCTCTGTTA ACTGTTCCTT GCCAAATGAA AATCAAACAA ACATCCCCTT TAATGAGTTG 
AACAAGTCTC TGCTCTACAG CGCTCCTATC GATCCTACAG AGTGGGTCGG CCTGCGGAAG 
TCTTCGCCTC TGCTAGTCTA CCTGAGGAAT AACCTCCTGA TGCTGGCTAT CCTGGCCTTT 
GAAGTCACCA TTTACCGCCA TCAGGAATAC TATCGAGGTC GAAATAACCT GACGGCCCCT 
GTGTCTAGAA CTATCTTTCA TGACATTACA AGACTACATC TAGATGATGG ACTTATTAAT 
TGTGCCAAAT ATTTCATTAA TTACTTCTTT TACAAGTTTG GTCTGGAGAC CTGTTTCCTA 
ATGTCAGTTA ACGTCATTGG CCAGCGAATG GATTTCTATG CCATGATCCA CGCCTGCTGG 
CTGATCGCTG TCTTATATAG ACGCAGAAGG AAAGCCATCG CAGAGATCTG GCCCAAGTAC 
TGCTGCTTCC TGGCATGCAT CATCACCTTC CAGTATTTCA TCTGCATTGG CATCCCACCT 
GCTCCTTGCC GAGATTACCC GTGGAGATTC AAGGGTGCCA GCTTCAATGA CAACATCATA 
AAGTGGCTGT ACTTCCCAGA TTTCATTGTG CGGCCCAACC CTGTGTTTCT CGTCTATGAC 
TTCATGCTGC TTCTGTGTGC CTCCTTACAA CGGCAGATTT TTGAGGATGA GAACAAGGCT 
GCAGTGCGAA TCATGGCAGG TGACAATGTC GAGATCTGCA TGAACCTTGA TGCGGCCTCC 
TTCAGCCAAC ATAACCCTGT GCCAGATTTT ATTCACTGCA GATCTTACTT AGACATGTCC 
AAAGTGATCA TCTTCAGCTA CCTCTTCTGG TTTGTGCTCA CCATCATCTT CATCACTGGG 
ACCACCAGGA TCAGCATCTT TTGCATGGGG TACCTGGTGG CCTGTTTCTA CTTCCTGCTC 
TTTGGGGGCG ATTTGCTGTT GAAACCCATC AAGAGCATCC TGCGCTACTG GGACTGGCTG 
ATCGCATACA ACGTTTTTGT GATTACGATG AAAAATATCC TGTCAATAGG AGCATGTGGA 
TACATTGGAA CATTGGTGCA CAATAGTTGT TGGTTGATCC AGGCTTTCAG CCTGGCCTGC 
ACAGTCAAAG GCTATCAAAT GCCTGCTGCT AATTCACCCT GTACACTTCC CAGTGGGGAA 
GCAGGAATCA TTTGGGACAG CATATGTTTT GCCTTCCTCC TGCTGCAAAG AAGAGTTTTC 
ATGAGTTATT ATTTTCTACA TGTTGTGGCT GATATAAAAG CTTCCCAGAT TCTGGCATCA 
AGAGGAGCTG AACTTTTCCA GGCCACAATT GTAAAAGCTG TAAAGGCAAG AATTGAGGAA 
GAGAAGAAGT CCATGGACCA GCTGAAGCGA CAGATGGATC GCATCAAGGC CAGGCAACAG 
AAATATAAAA AGGGTAAGGA GAGGATGCTG AGCTTGACCC AGGAGCCAGG GGAAGGCCAG 
GACATGCAAA AACTCTCTGA AGAGGATGAT GAAAGAGAAG CAGACAAACA GAAAGCCAAG 
GGCAAAAAAA AGCAGTGGTG GCGGCCTTGG GTTGATCATG CTTCCATGGT CAGGAGTGGA 
GATTATTATT TGTTTGAAAC GGATAGTGAA GAGGAGGAAG AGGAAGAATT AAAGAAGGAA 
GATGAAGAAC CTCCACGAAG GTCAGCATTC CAGTTTGTTT ATCAAGCCTG GATTACTGAT 
CCTAAAACAG CACTCCGACA AAGACACAAA GAGAAAAAAA GGTCTGCAAG AGAAGAACGG 
AAACGAAGGC GGAAAGGATC CAAGGAGGGT CCTGTGGAAT GGGAAGACCG GGAGGATGAA 
CCAATCAAAA AGAAATCCGA TGGACCAGAT AATATCATCA AGAGGATATT TAATATTTTG 
AAATTTACCT GGGTCCTATT TCTGGCAACA GTGGACAGTT TCACTACTTG GCTTAACTCC 
ATTTCAAGGG AGCATATTGA TATATCTACA GTTCTGAGAA TTGAACGATG CATGCTGACC 
AGAGAAATTA AGAAGGGCAA TGTTCCAACT CGGGAGAGCA TCCACATGTA CTATCAGAAC 
CACATCATGA ACCTTTCCAG AGAGTCGGGA CTGGACACCA TTGACGAGCA TCCCGGAGCT 
GCTTCAGGTG CACAGACAGC CCACAGGATG GATAGTTTAG ATTCACATGA CAGTATCTCC 
AGCGAGCCCA CGCAGTGTAC CATGCTGTAC TCACGCCAGG GGACCACTGA GACCATCGAG 
GAGGTGGAGG CTGAGCAGGA GGAGGAGGCA GGGAGCACGG CGCCTGAGCC CAGGGAGGCC 
AAGGAGTACG AGGCCACTGG GTACGATGTG GGAGCCATGG GTGCCGAGGA GGCCAGCCTC 
ACCCCAGAGG AAGAGCTGAC ACAGTTCTCC ACCTTGGACG GGGATGTGGA GGCCCCACCC 
TCCTACAGCA AGGCTGTGAG CTTCGAGCAT CTGTCCTTCG GCTCGCAGGA CGACTCTGCA 
GGCAAGAACC GTATGGCAGT CAGCCCGGAC GACAGCCGCA CCGACAAGCT GGGGTCCAGC 
ATCTTACCTC CCCTGACCCA TGAGCTGACG GCCAGCGAGC TGCTGCTGAA AAAGATGTTT 
CACGACGATG AGCTTGAAGA GTCAGAGAAA TTCTACGTGG GGCAGCCCCG ATTTCTGCTG 
CTCTTCTATG CCATGTACAA TACCCTGGTG GCCCGCTCGG AGATGGTGTG CTACTTCGTG 
ATCATCCTCA ACCACATGGT CTCTGCCTCC ATGATCACGC TCCTGCTTCC CATCCTCATC 
TTCCTCTGGG CCATGTTGTC CGTCCCCAGG CCCAGCCGCC GGTTCTGGAT GATGGCCATC 
GTCTATACTG AGGTGGCAAT TGTAGTCAAG TATTTCTTCC AATTTGGGTT CTTTCCCTGG 
AATAAGAATG TGGAGGTGAA CAAAGATAAA CCGTATCACC CCCCAAACAT CATAGGAGTG 
GAAAAGAAGG AAGGTTATGT TCTCTATGAC CTCATCCAGC TCCTGGCTCT GTTCTTTCAT 
CGATCAATTT TGAAGTGCCA TGGCTTATGG GATGAAGATG ACATGACTGA AAGTGGCATG 
GCCAGGGAGG AATCAGATGA TGAGCTCTCC CTCGGTCATG GCAGGAGGGA CTCCTCCGAT 
TCTCTCAAGT CCATCAACCT GGCCGCGTCT GTGGAGTCAG TGCATGTGAC CTTCCCGGAG 
CAGCAGACAG CTGTCCGGAG GAAGCGCTCC GGCAGCAGCT CCGAGCCATC CCAGAGATCC 
AGCTTTTCTT CAAACAGATC CCAAAGAGGC AGCACAAGCA CCCGAAACAG CAGTCAAAAA 
GGAAGCAGTG TTTTGAGTAT TAAGCAAAAA GGCAAAAGGG AACTTTATAT GGAAAAGCTT 
CAAGAACATT TAATCAAAGC AAAAGCCTTT ACCATAAAGA AGACGCTGGA GATCTATGTG 
CCCATCAAAC AGTTCTTTTA CAACCTCATC CACCCGGAGT ATAGCGCCGT GACTGACGTG 
TATGTACTCA TGTTCCTGGC TGACACTGTG GACTTCATCA TCATTGTCTT CGGCTTTTGG 
GCCTTTGGGA AACACTCAGC AGCTGCAGAC ATCACCTCTT CACTGTCAGA GGACCAGGTC 
CCGGGGCCGT TTTTGGTGAT GGTCCTCATT CAGTTTGGAA CCATGGTGGT GGACCGAGCC 
CTCTACCTCA GGAAGACTGT ACTGGGAAAG GTCATCTTCC AGGTCATTCT TGTGTTCGGA 
ATTCACTTCT GGATGTTCTT CATCTTACCT GGTGTGACTG AGAGGAAATT CAGCCAGAAC 
CTGGTTGCCC AGCTTTGGTA CTTTGTGAAA TGTGTTTACT TCGGGTTGTC TGCTTACCAG 
ATCCGTTGTG GCTACCCAAC GCGAGTCCTG GGGAACTTCC TCACCAAGAG CTACAATTAC 
GTCAACCTCT TCTTATTCCA AGGGTTTCGC CTCGTGCCCT TTTTGACTGA GCTGAGGGCA 
GTGATGGACT GGGTGTGGAC GGACACAACT TTGAGCCTGT CCAGCTGGAT CTGTGTGGAG 
GACATCTATG CTCACATATT CATCCTGAAG TGTTGGCGGG AGTCGGAGAA GAGATACCCT 
CAGCCACGGG GCCAGAAGAA GAAGAAAGTG GTGAAGTATG GCATGGGAGG AATGATCATC 
GTCCTGCTCA TCTGCATTGT CTGGTTTCCT CTTCTCTTCA TGTCTTTGAT CAAATCTGTG 
GCTGGGGTCA TCAACCAGCC CCTGGACGTC TCCGTCACAA TTACCCTGGG AGGGTATCAG 
CCTATTTTCA CAATGAGTGC CCAACAAAGC CAGTTGAAAG TTATGGACCA GCAGAGCTTT 
AACAAATTTA TACAAGCTTT TTCTAGGGAC ACCGGTGCTA TGCAATTTCT GGAAAATTAT 
GAAAAAGAAG ACATAACAGT AGCAGAACTG GAAGGAAACT CAAATTCTTT GTGGACCATC 
AGCCCACCCA GTAAGCAGAA AATGATACAC GAACTCCTGG ACCCCAATAG TAGCTTCTCT 
GTTGTTTTTT CATGGAGTAT TCAGAGAAAC TTAAGTCTGG GTGCAAAATC GGAAATAGCA 
ACAGATAAGC TTTCTTTTCC TCTTAAAAAT ATTACTCGAA AGAATATCGC TAAAATGATA 
GCAGGCAACA GCACAGAAAG TTCAAAAACA CCAGTGACCA TAGAAAAGAT TTATCCATAT 
TATGTGAAAG CACCTAGTGA TTCTAACTCA AAACCTATAA AGCAACTTTT ATCTGAAAAT 
AATTTCATGG ATATTACCAT CATTTTGTCC AGAGACAATA CAACTAAATA TAACAGTGAG 
TGGTGGGTTC TCAACCTGAC TGGAAACAGA ATATACAATC CGAACTCTCA GGCCCTGGAA 
CTGGTGGTCT TCAATGACAA AGTCAGTCCC CCAAGTCTGG GGTTCCTGGC TGGCTATGGT 
ATTATGGGAT TATATGCTTC AGTTGTCCTT GTGATTGGGA AATTTGTCCG TGAATTCTTC 
AGTGGGATTT CTCACTCCAT CATGTTTGAA GAGCTTCCAA ATGTGGATCG AATTTTGAAG 
TTGTGCACAG ATATTTTTTT AGTTCGAGAG ACAGGAGAAC TGGAGCTAGA AGAAGATCTC 
TATGCCAAAT TAATATTCCT ATATCGCTCA CCAGAGACAA TGATCAAATG GACTAGAGAA 
AAAACAAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PIEZO2
Entrez Gene ID	63895
Full Name	piezo type mechanosensitive ion channel component 2
Synonyms	C18orf30,C18orf58,DA3,DA5,DAIPT,FAM38B,FAM38B2,HsT748,HsT771,MWKS
General protein information	Preferred Names piezo type mechanosensitive ion channel component 2 Names piezo-type mechanosensitive ion channel component 2 PIEZO2 variant 16 family with sequence similarity 38, member A pseudogene family with sequence similarity 38, member B2 piezo-type mechanosensitive ion channel component 1 pseudogene protein PIEZO2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 18 Map Location: 18p11.22-p11.21
Summary	The protein encoded by this gene contains more than thirty transmembrane domains and likely functions as part of mechanically-activated (MA) cation channels. These channels serve to connect mechanical forces to biological signals. The encoded protein quickly adapts MA currents in somatosensory neurons. Defects in this gene are a cause of type 5 distal arthrogryposis. Several alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2014].
Disorder MIM:	613629
Disorder Html:	Arthrogryposis, distal, type 5, 108145 (3); Arthrogryposis, distal, type 3, 114300 (3); ?Marden-Walker syndrome, 248700 (3); Arthrogryposis, distal, with impaired proprioception and touch, 617146 (3)

Danio rerio (zebrafish)	LOC100534973	XP_003198010.2
Drosophila melanogaster (fruit fly)	Piezo	NP_609188.4
Homo sapiens (human)	PIEZO2	NP_071351.2
Pan troglodytes (chimpanzee)	PIEZO2	XP_003315863.2
Bos taurus (cattle)	PIEZO2	XP_003587835.2
Xenopus tropicalis (tropical clawed frog)	piezo2	XP_002937522.2
Mus musculus (house mouse)	Piezo2	NP_001034574.4
Rattus norvegicus (Norway rat)	Piezo2	XP_006254900.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009276	XP_320070.4
Caenorhabditis elegans (roundworm)	T20D3.11	NP_001255386.1
	LOC722206	XP_002800908.1
Canis lupus familiaris (dog)	PIEZO2	XP_005623199.1
Gallus gallus (chicken)	FAM38B	XP_004939783.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PIEZO2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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