CARD18 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CARD18
Entrez Gene ID	59082
Full Name	caspase recruitment domain family member 18
Synonyms	ICEBERG,UNQ5804,pseudo-ICE
General protein information	Preferred Names caspase recruitment domain family member 18 Names caspase recruitment domain-containing protein 18 ICEBERG caspase-1 inhibitor caspase-1 inhibitor Iceberg
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q22.3
Disorder MIM:	605354

mRNA and Protein(s)

mRNA	Protein	Name
NM_021571.3	NP_067546.1	caspase recruitment domain-containing protein 18
NM_021571.4	NP_067546.1	caspase recruitment domain-containing protein 18

Pathways from BioSystems

Homologies

Homo sapiens (human)	CARD18	NP_067546.1
	LOC705678	XP_001100470.1

Gene Ontology
Bibliography

Related articles in PubMed

The caspase-1 inhibitor CARD18 is specifically expressed during late differentiation of keratinocytes and its expression is lost in lichen planus.
Qin H, Jin J, Fischer H, Mildner M, Gschwandtner M, Mlitz V, Eckhart L, Tschachler E
Journal of dermatological science87(2)176-182(2017 Aug)

Molecular basis of caspase-1 polymerization and its inhibition by a new capping mechanism.
Lu A, Li Y, Schmidt FI, Yin Q, Chen S, Fu TM, Tong AB, Ploegh HL, Mao Y, Wu H
Nature structural & molecular biology23(5)416-25(2016 05)

Keratinocytes express functional CARD18, a negative regulator of inflammasome activation, and its altered expression in psoriasis may contribute to disease pathogenesis.
G?bl?s A, Danis J, Vas K, Bata-Cs?rg? Z, Kem?ny L, Sz?ll M
Molecular immunology7310-8(2016 05)

[The expression of CARD18 in apoptin-transfected gastric cancer cells and gastric adenocarcinoma tissues].
Tan C, Liu S, Xiang Z
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology29(8)858-61(2013 Aug)

Single nucleotide polymorphisms of matrix metalloproteinase 9 (MMP9) and tumor protein 73 (TP73) interact with Epstein-Barr virus in chronic lymphocytic leukemia: results from the European case-control study EpiLymph.
Casabonne D, Reina O, Benavente Y, Becker N, Maynadi? M, Foretov? L, Cocco P, Gonz?lez-Neira A, Nieters A, Boffetta P, Middeldorp JM, de Sanjose S
Haematologica96(2)323-7(2011 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CARD18 expression differs in healthy and psoriatic skin; moreover, CARD18 demonstrated altered response under inflammatory conditions in healthy and psoriatic skin
Title: Keratinocytes express functional CARD18, a negative regulator of inflammasome activation, and its altered expression in psoriasis may contribute to disease pathogenesis.

CARD18 may be both a promising marker for prognosis and a target protein for treatment of gastric cancer.
Title: [The expression of CARD18 in apoptin-transfected gastric cancer cells and gastric adenocarcinoma tissues].

Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Single nucleotide polymorphisms of matrix metalloproteinase 9 (MMP9) and tumor protein 73 (TP73) interact with Epstein-Barr virus in chronic lymphocytic leukemia: results from the European case-control study EpiLymph.

The following CARD18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CARD18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu32314	NM_021571.4 Latest version!	Homo sapiens caspase recruitment domain family member 18 (CARD18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu32314	NM_021571.3	Homo sapiens caspase recruitment domain family member 18 (CARD18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CARD18 ( NM_021571.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_067546.1 Homo sapiens caspase recruitment domain family member 18 (CARD18), mRNA.

Price & Availability↑

CloneID	OHu32314
Clone ID Related Accession (Same CDS sequence)	NM_021571.3 , NM_021571.4
Accession Version	NM_021571.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	caspase recruitment domain-containing protein 18
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY358231.1, AP003181.2 and AW295205.1. On Oct 9, 2009 this sequence version replaced NM_021571.2. ##Evidence-Data-START## Transcript exon combination :: AY358231.1, AA257158.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145893, SAMEA2147596 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGACC AACTCTTGCG TAAAAAGAGA AGAATTTTTA TCCATTCAGT GGGTGCAGGC 
ACAATAAATG CCTTGCTGGA TTGCCTATTA GAGGATGAAG TTATTAGCCA GGAAGACATG 
AACAAAGTGA GAGATGAAAA TGACACTGTC ATGGATAAGG CTCGAGTCTT GATTGACCTT 
GTTACTGGAA AAGGACCCAA GTCTTGCTGC AAATTTATCA AGCATCTCTG TGAAGAAGAC 
CCTCAACTTG CCTCAAAGAT GGGTTTGCAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067546.1
CDS	9..281
Translation	MADQLLRKKRRIFIHSVGAGTINALLDCLLEDEVISQEDMNKVRDENDTVMDKARVLIDLVTGKGPKSCCKFIKHLCEEDPQLASKMGLH

Target ORF information:

RefSeq Version	NM_021571.3
Organism	Homo sapiens(human)
Definition	Homo sapiens caspase recruitment domain family member 18 (CARD18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021571.3

ORF Insert Sequence:

ATGGCTGACC AACTCTTGCG TAAAAAGAGA AGAATTTTTA TCCATTCAGT GGGTGCAGGC 
ACAATAAATG CCTTGCTGGA TTGCCTATTA GAGGATGAAG TTATTAGCCA GGAAGACATG 
AACAAAGTGA GAGATGAAAA TGACACTGTC ATGGATAAGG CTCGAGTCTT GATTGACCTT 
GTTACTGGAA AAGGACCCAA GTCTTGCTGC AAATTTATCA AGCATCTCTG TGAAGAAGAC 
CCTCAACTTG CCTCAAAGAT GGGTTTGCAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CARD18 ( NM_021571.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_067546.1 Homo sapiens caspase recruitment domain family member 18 (CARD18), mRNA.

Price & Availability↑

CloneID	OHu32314
Clone ID Related Accession (Same CDS sequence)	NM_021571.3 , NM_021571.4
Accession Version	NM_021571.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	caspase recruitment domain-containing protein 18
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP003181.2, AY358231.1 and AW295205.1. On May 17, 2019 this sequence version replaced NM_021571.3. ##Evidence-Data-START## Transcript exon combination :: AY358231.1, AA257158.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145893, SAMEA2147596 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000530950.2/ ENSP00000436691.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGACC AACTCTTGCG TAAAAAGAGA AGAATTTTTA TCCATTCAGT GGGTGCAGGC 
ACAATAAATG CCTTGCTGGA TTGCCTATTA GAGGATGAAG TTATTAGCCA GGAAGACATG 
AACAAAGTGA GAGATGAAAA TGACACTGTC ATGGATAAGG CTCGAGTCTT GATTGACCTT 
GTTACTGGAA AAGGACCCAA GTCTTGCTGC AAATTTATCA AGCATCTCTG TGAAGAAGAC 
CCTCAACTTG CCTCAAAGAT GGGTTTGCAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067546.1
CDS	44..316
Translation	MADQLLRKKRRIFIHSVGAGTINALLDCLLEDEVISQEDMNKVRDENDTVMDKARVLIDLVTGKGPKSCCKFIKHLCEEDPQLASKMGLH

Target ORF information:

RefSeq Version	NM_021571.4
Organism	Homo sapiens(human)
Definition	Homo sapiens caspase recruitment domain family member 18 (CARD18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021571.4

ORF Insert Sequence:

ATGGCTGACC AACTCTTGCG TAAAAAGAGA AGAATTTTTA TCCATTCAGT GGGTGCAGGC 
ACAATAAATG CCTTGCTGGA TTGCCTATTA GAGGATGAAG TTATTAGCCA GGAAGACATG 
AACAAAGTGA GAGATGAAAA TGACACTGTC ATGGATAAGG CTCGAGTCTT GATTGACCTT 
GTTACTGGAA AAGGACCCAA GTCTTGCTGC AAATTTATCA AGCATCTCTG TGAAGAAGAC 
CCTCAACTTG CCTCAAAGAT GGGTTTGCAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The caspase-1 inhibitor CARD18 is specifically expressed during late differentiation of keratinocytes and its expression is lost in lichen planus.
Journal of dermatological science87(2)176-182(2017 Aug)
Qin H,Jin J,Fischer H,Mildner M,Gschwandtner M,Mlitz V,Eckhart L,Tschachler E

Molecular basis of caspase-1 polymerization and its inhibition by a new capping mechanism.
Nature structural & molecular biology23(5)416-25(2016 05)
Lu A,Li Y,Schmidt FI,Yin Q,Chen S,Fu TM,Tong AB,Ploegh HL,Mao Y,Wu H

Keratinocytes express functional CARD18, a negative regulator of inflammasome activation, and its altered expression in psoriasis may contribute to disease pathogenesis.
Molecular immunology7310-8(2016 05)
G?bl?s A,Danis J,Vas K,Bata-Cs?rg? Z,Kem?ny L,Sz?ll M

[The expression of CARD18 in apoptin-transfected gastric cancer cells and gastric adenocarcinoma tissues].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology29(8)858-61(2013 Aug)
Tan C,Liu S,Xiang Z

Single nucleotide polymorphisms of matrix metalloproteinase 9 (MMP9) and tumor protein 73 (TP73) interact with Epstein-Barr virus in chronic lymphocytic leukemia: results from the European case-control study EpiLymph.
Haematologica96(2)323-7(2011 Feb)
Casabonne D,Reina O,Benavente Y,Becker N,Maynadi? M,Foretov? L,Cocco P,Gonz?lez-Neira A,Nieters A,Boffetta P,Middeldorp JM,de Sanjose S