USP29 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	USP29
Entrez Gene ID	57663
Full Name	ubiquitin specific peptidase 29
Synonyms	HOM-TES-84/86
General protein information	Preferred Names ubiquitin specific peptidase 29 Names ubiquitin carboxyl-terminal hydrolase 29 deubiquitinating enzyme 29 ubiquitin thioesterase 29 ubiquitin thiolesterase 29 ubiquitin-specific processing protease ubiquitin-specific-processing protease 29
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.43
Disorder MIM:	609546

mRNA and Protein(s)

mRNA	Protein	Name
NM_020903.2	NP_065954.1	ubiquitin carboxyl-terminal hydrolase 29
XM_024451624.1	XP_024307392.1	ubiquitin carboxyl-terminal hydrolase 29 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	USP29	XP_001141802.2
Mus musculus (house mouse)	Usp29	NP_067298.2
Rattus norvegicus (Norway rat)	Usp29	NP_001101935.1
	USP29	XP_001092089.1
Homo sapiens (human)	USP29	NP_065954.1

Gene Ontology
Bibliography

Related articles in PubMed

USP29 controls the stability of checkpoint adaptor Claspin by deubiquitination.
Mart?n Y, Cabrera E, Amoedo H, Hern?ndez-P?rez S, Dom?nguez-Kelly R, Freire R
Oncogene34(8)1058-63(2015 Feb)

Defining the human deubiquitinating enzyme interaction landscape.
Sowa ME, Bennett EJ, Gygi SP, Harper JW
Cell138(2)389-403(2009 Jul)

Human and mouse proteases: a comparative genomic approach.
Puente XS, S?nchez LM, Overall CM, L?pez-Ot?n C
Nature reviews. Genetics4(7)544-58(2003 Jul)

A novel tumour associated leucine zipper protein targeting to sites of gene transcription and splicing.
T?reci O, Sahin U, Koslowski M, Buss B, Bell C, Ballweber P, Zwick C, Eberle T, Zuber M, Villena-Heinsen C, Seitz G, Pfreundschuh M
Oncogene21(24)3879-88(2002 May)

Discovery of a novel, paternally expressed ubiquitin-specific processing protease gene through comparative analysis of an imprinted region of mouse chromosome 7 and human chromosome 19q13.4.
Kim J, Noskov VN, Lu X, Bergmann A, Ren X, Warth T, Richardson P, Kouprina N, Stubbs L
Genome research10(8)1138-47(2000 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

USP29 interacts with Claspin and is able to deubiquitinate it both in vivo and in vitro.
Title: USP29 controls the stability of checkpoint adaptor Claspin by deubiquitination.

The following USP29 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP29 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu30242	NM_020903.2 Latest version!	Homo sapiens ubiquitin specific peptidase 29 (USP29), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu30242	XM_024451624.1 Latest version!	Homo sapiens ubiquitin specific peptidase 29 (USP29), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

USP29 ( NM_020903.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_065954.1 Homo sapiens ubiquitin specific peptidase 29 (USP29), mRNA.

Price & Availability↑

CloneID	OHu30242
Clone ID Related Accession (Same CDS sequence)	NM_020903.2 , XM_024451624.1
Accession Version	NM_020903.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 29
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF124433.1 and AF229438.1. On Dec 23, 2004 this sequence version replaced NM_020903.1. ##Evidence-Data-START## Transcript exon combination :: AK308285.1, DB086580.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATATCTC TAAAGGTATG TGGATTCATC CAAATTTGGA GCCAGAAGAC TGGGATGACT 
AAGCTGAAAG AAGCTCTCAT TGAAACAGTG CAAAGACAAA AGGAAATTAA ACTGGTGGTC 
ACTTTCAAAT CTGGAAAATT TATAAGAATT TTTCAGCTGA GCAACAACAT TAGAAGTGTG 
GTCCTTAGAC ATTGTAAAAA AAGACAAAGT CACCTGCGTT TAACTTTGAA AAACAACGTG 
TTCTTGTTTA TTGACAAATT ATCCTACAGA GATGCTAAAC AGTTGAATAT GTTCCTGGAC 
ATAATCCACC AAAACAAATC TCAGCAACCC ATGAAATCTG ATGATGATTG GAGTGTGTTT 
GAAAGCAGGA ATATGCTGAA GGAAATTGAC AAAACTTCAT TTTACAGCAT TTGTAACAAG 
CCAAGTTATC AGAAGATGCC TTTGTTTATG TCAAAATCAC CAACACATGT GAAAAAGGGG 
ATATTAGAAA ATCAAGGTGG GAAGGGGCAA AACACACTAT CATCTGATGT ACAGACAAAT 
GAGGACATTC TGAAGGAAGA TAACCCTGTA CCAAACAAGA AATATAAGAC AGATTCCTTG 
AAATATATAC AAAGCAATAG GAAGAACCCA TCAAGTTTAG AGGATTTAGA AAAAGATAGA 
GATTTGAAAC TCGGGCCTTC ATTCAATACC AACTGTAATG GAAATCCTAA CCTAGATGAG 
ACTGTTCTTG CAACCCAGAC TCTCAATGCC AAAAATGGTT TGACATCTCC ATTGGAACCA 
GAGCACAGCC AGGGTGACCC AAGATGCAAC AAAGCCCAGG TGCCTCTTGA CTCTCATTCA 
CAGCAACTGC AGCAGGGGTT CCCCAATTTG GGAAACACCT GTTACATGAA TGCAGTTTTA 
CAATCGCTAT TTGCAATTCC ATCTTTTGCT GATGACTTAC TCACTCAAGG TGTCCCATGG 
GAATATATTC CCTTTGAGGC TCTTATTATG ACCTTGACCC AGCTGCTTGC TTTGAAAGAT 
TTCTGTAGTA CAAAGATCAA GAGAGAATTA CTTGGGAATG TTAAAAAAGT CATTTCAGCA 
GTTGCAGAAA TATTTTCTGG CAACATGCAG AATGATGCTC ATGAGTTTTT AGGTCAGTGT 
TTAGACCAGC TGAAAGAAGA CATGGAAAAA TTAAATGCCA CTTTGAATAC TGGGAAAGAA 
TGTGGGGATG AAAATTCATC TCCACAAATG CATGTTGGTA GTGCTGCCAC CAAAGTGTTT 
GTTTGCCCTG TTGTTGCTAA TTTTGAGTTT GAATTGCAGC TCTCCCTTAT TTGTAAAGCT 
TGTGGTCATG CTGTTCTCAA GGTAGAACCT AATAATTATC TCTCCATCAA CCTGCACCAA 
GAAACAAAAC CACTTCCTTT GTCCATTCAG AATTCTTTAG ATCTTTTCTT TAAAGAAGAA 
GAGCTTGAAT ATAACTGTCA GATGTGTAAG CAGAAGAGTT GTGTTGCAAG GCATACATTT 
AGTAGGCTCT CCAGGGTCCT TATCATTCAT CTGAAACGCT ATAGCTTCAA CAATGCTTGG 
TTGCTGGTGA AGAATAACGA GCAAGTTTAT ATTCCCAAAT CTTTAAGTTT ATCTTCTTAT 
TGCAATGAAA GCACCAAACC ACCTCTTCCC TTGAGCAGTA GTGCACCTGT TGGGAAATGT 
GAAGTCCTGG AAGTCTCTCA GGAGATGATT TCTGAGATCA ACAGCCCATT GACACCATCA 
ATGAAGCTGA CCTCAGAATC CAGTGATTCC CTGGTTCTAC CCGTTGAACC AGACAAGAAT 
GCCGACCTAC AAAGATTCCA GAGAGACTGT GGAGATGCAA GCCAAGAGCA GCATCAGAGA 
GACCTGGAAA ATGGCTCTGC ACTAGAGTCA GAATTGGTCC ACTTTAGAGA TAGGGCAATC 
GGTGAAAAGG AGCTTCCAGT GGCTGACTCA CTGATGGACC AGGGAGACAT TTCTCTTCCT 
GTGATGTATG AAGATGGAGG GAAGCTGATC AGCAGCCCAG ACACAAGGCT TGTCGAGGTT 
CATCTTCAAG AGGTGCCTCA ACATCCAGAA CTTCAGAAGT ATGAGAAAAC CAATACATTC 
GTAGAGTTCA ATTTTGACAG TGTCACTGAG TCCACCAATG GCTTTTATGA CTGTAAAGAA 
AACAGGATTC CAGAAGGATC TCAAGGAATG GCTGAACAGC TCCAGCAGTG TATTGAGGAG 
AGCATCATAG ATGAATTTCT TCAGCAGGCA CCACCTCCAG GTGTTAGGAA GCTGGATGCC 
CAGGAACATA CAGAAGAGAC CCTCAATCAG TCTACAGAAT TAAGACTTCA AAAGGCTGAC 
CTGAATCACC TTGGGGCACT GGGTTCTGAC AACCCAGGAA ACAAAAACAT TTTAGATGCA 
GAGAACACAA GAGGTGAAGC CAAGGAACTA ACAAGAAACG TGAAGATGGG GGATCCTCTC 
CAGGCCTACA GACTCATCAG TGTTGTCAGC CATATCGGGA GCTCCCCAAA TTCAGGCCAT 
TACATCAGCG ATGTGTATGA CTTTCAGAAG CAGGCCTGGT TCACATACAA CGATCTATGT 
GTATCAGAAA TCTCAGAGAC CAAAATGCAG GAGGCGAGGC TTCACTCTGG GTATATCTTC 
TTTTACATGC ACAATGGGAT TTTTGAGGAG CTGTTAAGAA AAGCAGAGAA CTCTCGGCTA 
CCTAGCACAC AGGCAGGGGT GATCCCTCAG GGGGAATACG AAGGTGACTC TTTGTACAGA 
CCTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065954.1
CDS	357..3125
Translation	MISLKVCGFIQIWSQKTGMTKLKEALIETVQRQKEIKLVVTFKSGKFIRIFQLSNNIRSVVLRHCKKRQSHLRLTLKNNVFLFIDKLSYRDAKQLNMFLDIIHQNKSQQPMKSDDDWSVFESRNMLKEIDKTSFYSICNKPSYQKMPLFMSKSPTHVKKGILENQGGKGQNTLSSDVQTNEDILKEDNPVPNKKYKTDSLKYIQSNRKNPSSLEDLEKDRDLKLGPSFNTNCNGNPNLDETVLATQTLNAKNGLTSPLEPEHSQGDPRCNKAQVPLDSHSQQLQQGFPNLGNTCYMNAVLQSLFAIPSFADDLLTQGVPWEYIPFEALIMTLTQLLALKDFCSTKIKRELLGNVKKVISAVAEIFSGNMQNDAHEFLGQCLDQLKEDMEKLNATLNTGKECGDENSSPQMHVGSAATKVFVCPVVANFEFELQLSLICKACGHAVLKVEPNNYLSINLHQETKPLPLSIQNSLDLFFKEEELEYNCQMCKQKSCVARHTFSRLSRVLIIHLKRYSFNNAWLLVKNNEQVYIPKSLSLSSYCNESTKPPLPLSSSAPVGKCEVLEVSQEMISEINSPLTPSMKLTSESSDSLVLPVEPDKNADLQRFQRDCGDASQEQHQRDLENGSALESELVHFRDRAIGEKELPVADSLMDQGDISLPVMYEDGGKLISSPDTRLVEVHLQEVPQHPELQKYEKTNTFVEFNFDSVTESTNGFYDCKENRIPEGSQGMAEQLQQCIEESIIDEFLQQAPPPGVRKLDAQEHTEETLNQSTELRLQKADLNHLGALGSDNPGNKNILDAENTRGEAKELTRNVKMGDPLQAYRLISVVSHIGSSPNSGHYISDVYDFQKQAWFTYNDLCVSEISETKMQEARLHSGYIFFYMHNGIFEELLRKAENSRLPSTQAGVIPQGEYEGDSLYRPA

Target ORF information:

RefSeq Version	NM_020903.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 29 (USP29), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020903.2

ORF Insert Sequence:

ATGATATCTC TAAAGGTATG TGGATTCATC CAAATTTGGA GCCAGAAGAC TGGGATGACT 
AAGCTGAAAG AAGCTCTCAT TGAAACAGTG CAAAGACAAA AGGAAATTAA ACTGGTGGTC 
ACTTTCAAAT CTGGAAAATT TATAAGAATT TTTCAGCTGA GCAACAACAT TAGAAGTGTG 
GTCCTTAGAC ATTGTAAAAA AAGACAAAGT CACCTGCGTT TAACTTTGAA AAACAACGTG 
TTCTTGTTTA TTGACAAATT ATCCTACAGA GATGCTAAAC AGTTGAATAT GTTCCTGGAC 
ATAATCCACC AAAACAAATC TCAGCAACCC ATGAAATCTG ATGATGATTG GAGTGTGTTT 
GAAAGCAGGA ATATGCTGAA GGAAATTGAC AAAACTTCAT TTTACAGCAT TTGTAACAAG 
CCAAGTTATC AGAAGATGCC TTTGTTTATG TCAAAATCAC CAACACATGT GAAAAAGGGG 
ATATTAGAAA ATCAAGGTGG GAAGGGGCAA AACACACTAT CATCTGATGT ACAGACAAAT 
GAGGACATTC TGAAGGAAGA TAACCCTGTA CCAAACAAGA AATATAAGAC AGATTCCTTG 
AAATATATAC AAAGCAATAG GAAGAACCCA TCAAGTTTAG AGGATTTAGA AAAAGATAGA 
GATTTGAAAC TCGGGCCTTC ATTCAATACC AACTGTAATG GAAATCCTAA CCTAGATGAG 
ACTGTTCTTG CAACCCAGAC TCTCAATGCC AAAAATGGTT TGACATCTCC ATTGGAACCA 
GAGCACAGCC AGGGTGACCC AAGATGCAAC AAAGCCCAGG TGCCTCTTGA CTCTCATTCA 
CAGCAACTGC AGCAGGGGTT CCCCAATTTG GGAAACACCT GTTACATGAA TGCAGTTTTA 
CAATCGCTAT TTGCAATTCC ATCTTTTGCT GATGACTTAC TCACTCAAGG TGTCCCATGG 
GAATATATTC CCTTTGAGGC TCTTATTATG ACCTTGACCC AGCTGCTTGC TTTGAAAGAT 
TTCTGTAGTA CAAAGATCAA GAGAGAATTA CTTGGGAATG TTAAAAAAGT CATTTCAGCA 
GTTGCAGAAA TATTTTCTGG CAACATGCAG AATGATGCTC ATGAGTTTTT AGGTCAGTGT 
TTAGACCAGC TGAAAGAAGA CATGGAAAAA TTAAATGCCA CTTTGAATAC TGGGAAAGAA 
TGTGGGGATG AAAATTCATC TCCACAAATG CATGTTGGTA GTGCTGCCAC CAAAGTGTTT 
GTTTGCCCTG TTGTTGCTAA TTTTGAGTTT GAATTGCAGC TCTCCCTTAT TTGTAAAGCT 
TGTGGTCATG CTGTTCTCAA GGTAGAACCT AATAATTATC TCTCCATCAA CCTGCACCAA 
GAAACAAAAC CACTTCCTTT GTCCATTCAG AATTCTTTAG ATCTTTTCTT TAAAGAAGAA 
GAGCTTGAAT ATAACTGTCA GATGTGTAAG CAGAAGAGTT GTGTTGCAAG GCATACATTT 
AGTAGGCTCT CCAGGGTCCT TATCATTCAT CTGAAACGCT ATAGCTTCAA CAATGCTTGG 
TTGCTGGTGA AGAATAACGA GCAAGTTTAT ATTCCCAAAT CTTTAAGTTT ATCTTCTTAT 
TGCAATGAAA GCACCAAACC ACCTCTTCCC TTGAGCAGTA GTGCACCTGT TGGGAAATGT 
GAAGTCCTGG AAGTCTCTCA GGAGATGATT TCTGAGATCA ACAGCCCATT GACACCATCA 
ATGAAGCTGA CCTCAGAATC CAGTGATTCC CTGGTTCTAC CCGTTGAACC AGACAAGAAT 
GCCGACCTAC AAAGATTCCA GAGAGACTGT GGAGATGCAA GCCAAGAGCA GCATCAGAGA 
GACCTGGAAA ATGGCTCTGC ACTAGAGTCA GAATTGGTCC ACTTTAGAGA TAGGGCAATC 
GGTGAAAAGG AGCTTCCAGT GGCTGACTCA CTGATGGACC AGGGAGACAT TTCTCTTCCT 
GTGATGTATG AAGATGGAGG GAAGCTGATC AGCAGCCCAG ACACAAGGCT TGTCGAGGTT 
CATCTTCAAG AGGTGCCTCA ACATCCAGAA CTTCAGAAGT ATGAGAAAAC CAATACATTC 
GTAGAGTTCA ATTTTGACAG TGTCACTGAG TCCACCAATG GCTTTTATGA CTGTAAAGAA 
AACAGGATTC CAGAAGGATC TCAAGGAATG GCTGAACAGC TCCAGCAGTG TATTGAGGAG 
AGCATCATAG ATGAATTTCT TCAGCAGGCA CCACCTCCAG GTGTTAGGAA GCTGGATGCC 
CAGGAACATA CAGAAGAGAC CCTCAATCAG TCTACAGAAT TAAGACTTCA AAAGGCTGAC 
CTGAATCACC TTGGGGCACT GGGTTCTGAC AACCCAGGAA ACAAAAACAT TTTAGATGCA 
GAGAACACAA GAGGTGAAGC CAAGGAACTA ACAAGAAACG TGAAGATGGG GGATCCTCTC 
CAGGCCTACA GACTCATCAG TGTTGTCAGC CATATCGGGA GCTCCCCAAA TTCAGGCCAT 
TACATCAGCG ATGTGTATGA CTTTCAGAAG CAGGCCTGGT TCACATACAA CGATCTATGT 
GTATCAGAAA TCTCAGAGAC CAAAATGCAG GAGGCGAGGC TTCACTCTGG GTATATCTTC 
TTTTACATGC ACAATGGGAT TTTTGAGGAG CTGTTAAGAA AAGCAGAGAA CTCTCGGCTA 
CCTAGCACAC AGGCAGGGGT GATCCCTCAG GGGGAATACG AAGGTGACTC TTTGTACAGA 
CCTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP29 ( XM_024451624.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307392.1 Homo sapiens ubiquitin specific peptidase 29 (USP29), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu30242
Clone ID Related Accession (Same CDS sequence)	NM_020903.2 , XM_024451624.1
Accession Version	XM_024451624.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 29 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATCTC TAAAGGTATG TGGATTCATC CAAATTTGGA GCCAGAAGAC TGGGATGACT 
AAGCTGAAAG AAGCTCTCAT TGAAACAGTG CAAAGACAAA AGGAAATTAA ACTGGTGGTC 
ACTTTCAAAT CTGGAAAATT TATAAGAATT TTTCAGCTGA GCAACAACAT TAGAAGTGTG 
GTCCTTAGAC ATTGTAAAAA AAGACAAAGT CACCTGCGTT TAACTTTGAA AAACAACGTG 
TTCTTGTTTA TTGACAAATT ATCCTACAGA GATGCTAAAC AGTTGAATAT GTTCCTGGAC 
ATAATCCACC AAAACAAATC TCAGCAACCC ATGAAATCTG ATGATGATTG GAGTGTGTTT 
GAAAGCAGGA ATATGCTGAA GGAAATTGAC AAAACTTCAT TTTACAGCAT TTGTAACAAG 
CCAAGTTATC AGAAGATGCC TTTGTTTATG TCAAAATCAC CAACACATGT GAAAAAGGGG 
ATATTAGAAA ATCAAGGTGG GAAGGGGCAA AACACACTAT CATCTGATGT ACAGACAAAT 
GAGGACATTC TGAAGGAAGA TAACCCTGTA CCAAACAAGA AATATAAGAC AGATTCCTTG 
AAATATATAC AAAGCAATAG GAAGAACCCA TCAAGTTTAG AGGATTTAGA AAAAGATAGA 
GATTTGAAAC TCGGGCCTTC ATTCAATACC AACTGTAATG GAAATCCTAA CCTAGATGAG 
ACTGTTCTTG CAACCCAGAC TCTCAATGCC AAAAATGGTT TGACATCTCC ATTGGAACCA 
GAGCACAGCC AGGGTGACCC AAGATGCAAC AAAGCCCAGG TGCCTCTTGA CTCTCATTCA 
CAGCAACTGC AGCAGGGGTT CCCCAATTTG GGAAACACCT GTTACATGAA TGCAGTTTTA 
CAATCGCTAT TTGCAATTCC ATCTTTTGCT GATGACTTAC TCACTCAAGG TGTCCCATGG 
GAATATATTC CCTTTGAGGC TCTTATTATG ACCTTGACCC AGCTGCTTGC TTTGAAAGAT 
TTCTGTAGTA CAAAGATCAA GAGAGAATTA CTTGGGAATG TTAAAAAAGT CATTTCAGCA 
GTTGCAGAAA TATTTTCTGG CAACATGCAG AATGATGCTC ATGAGTTTTT AGGTCAGTGT 
TTAGACCAGC TGAAAGAAGA CATGGAAAAA TTAAATGCCA CTTTGAATAC TGGGAAAGAA 
TGTGGGGATG AAAATTCATC TCCACAAATG CATGTTGGTA GTGCTGCCAC CAAAGTGTTT 
GTTTGCCCTG TTGTTGCTAA TTTTGAGTTT GAATTGCAGC TCTCCCTTAT TTGTAAAGCT 
TGTGGTCATG CTGTTCTCAA GGTAGAACCT AATAATTATC TCTCCATCAA CCTGCACCAA 
GAAACAAAAC CACTTCCTTT GTCCATTCAG AATTCTTTAG ATCTTTTCTT TAAAGAAGAA 
GAGCTTGAAT ATAACTGTCA GATGTGTAAG CAGAAGAGTT GTGTTGCAAG GCATACATTT 
AGTAGGCTCT CCAGGGTCCT TATCATTCAT CTGAAACGCT ATAGCTTCAA CAATGCTTGG 
TTGCTGGTGA AGAATAACGA GCAAGTTTAT ATTCCCAAAT CTTTAAGTTT ATCTTCTTAT 
TGCAATGAAA GCACCAAACC ACCTCTTCCC TTGAGCAGTA GTGCACCTGT TGGGAAATGT 
GAAGTCCTGG AAGTCTCTCA GGAGATGATT TCTGAGATCA ACAGCCCATT GACACCATCA 
ATGAAGCTGA CCTCAGAATC CAGTGATTCC CTGGTTCTAC CCGTTGAACC AGACAAGAAT 
GCCGACCTAC AAAGATTCCA GAGAGACTGT GGAGATGCAA GCCAAGAGCA GCATCAGAGA 
GACCTGGAAA ATGGCTCTGC ACTAGAGTCA GAATTGGTCC ACTTTAGAGA TAGGGCAATC 
GGTGAAAAGG AGCTTCCAGT GGCTGACTCA CTGATGGACC AGGGAGACAT TTCTCTTCCT 
GTGATGTATG AAGATGGAGG GAAGCTGATC AGCAGCCCAG ACACAAGGCT TGTCGAGGTT 
CATCTTCAAG AGGTGCCTCA ACATCCAGAA CTTCAGAAGT ATGAGAAAAC CAATACATTC 
GTAGAGTTCA ATTTTGACAG TGTCACTGAG TCCACCAATG GCTTTTATGA CTGTAAAGAA 
AACAGGATTC CAGAAGGATC TCAAGGAATG GCTGAACAGC TCCAGCAGTG TATTGAGGAG 
AGCATCATAG ATGAATTTCT TCAGCAGGCA CCACCTCCAG GTGTTAGGAA GCTGGATGCC 
CAGGAACATA CAGAAGAGAC CCTCAATCAG TCTACAGAAT TAAGACTTCA AAAGGCTGAC 
CTGAATCACC TTGGGGCACT GGGTTCTGAC AACCCAGGAA ACAAAAACAT TTTAGATGCA 
GAGAACACAA GAGGTGAAGC CAAGGAACTA ACAAGAAACG TGAAGATGGG GGATCCTCTC 
CAGGCCTACA GACTCATCAG TGTTGTCAGC CATATCGGGA GCTCCCCAAA TTCAGGCCAT 
TACATCAGCG ATGTGTATGA CTTTCAGAAG CAGGCCTGGT TCACATACAA CGATCTATGT 
GTATCAGAAA TCTCAGAGAC CAAAATGCAG GAGGCGAGGC TTCACTCTGG GTATATCTTC 
TTTTACATGC ACAATGGGAT TTTTGAGGAG CTGTTAAGAA AAGCAGAGAA CTCTCGGCTA 
CCTAGCACAC AGGCAGGGGT GATCCCTCAG GGGGAATACG AAGGTGACTC TTTGTACAGA 
CCTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307392.1
CDS	720..3488
Translation	MISLKVCGFIQIWSQKTGMTKLKEALIETVQRQKEIKLVVTFKSGKFIRIFQLSNNIRSVVLRHCKKRQSHLRLTLKNNVFLFIDKLSYRDAKQLNMFLDIIHQNKSQQPMKSDDDWSVFESRNMLKEIDKTSFYSICNKPSYQKMPLFMSKSPTHVKKGILENQGGKGQNTLSSDVQTNEDILKEDNPVPNKKYKTDSLKYIQSNRKNPSSLEDLEKDRDLKLGPSFNTNCNGNPNLDETVLATQTLNAKNGLTSPLEPEHSQGDPRCNKAQVPLDSHSQQLQQGFPNLGNTCYMNAVLQSLFAIPSFADDLLTQGVPWEYIPFEALIMTLTQLLALKDFCSTKIKRELLGNVKKVISAVAEIFSGNMQNDAHEFLGQCLDQLKEDMEKLNATLNTGKECGDENSSPQMHVGSAATKVFVCPVVANFEFELQLSLICKACGHAVLKVEPNNYLSINLHQETKPLPLSIQNSLDLFFKEEELEYNCQMCKQKSCVARHTFSRLSRVLIIHLKRYSFNNAWLLVKNNEQVYIPKSLSLSSYCNESTKPPLPLSSSAPVGKCEVLEVSQEMISEINSPLTPSMKLTSESSDSLVLPVEPDKNADLQRFQRDCGDASQEQHQRDLENGSALESELVHFRDRAIGEKELPVADSLMDQGDISLPVMYEDGGKLISSPDTRLVEVHLQEVPQHPELQKYEKTNTFVEFNFDSVTESTNGFYDCKENRIPEGSQGMAEQLQQCIEESIIDEFLQQAPPPGVRKLDAQEHTEETLNQSTELRLQKADLNHLGALGSDNPGNKNILDAENTRGEAKELTRNVKMGDPLQAYRLISVVSHIGSSPNSGHYISDVYDFQKQAWFTYNDLCVSEISETKMQEARLHSGYIFFYMHNGIFEELLRKAENSRLPSTQAGVIPQGEYEGDSLYRPA

Target ORF information:

RefSeq Version	XM_024451624.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 29 (USP29), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451624.1

ORF Insert Sequence:

ATGATATCTC TAAAGGTATG TGGATTCATC CAAATTTGGA GCCAGAAGAC TGGGATGACT 
AAGCTGAAAG AAGCTCTCAT TGAAACAGTG CAAAGACAAA AGGAAATTAA ACTGGTGGTC 
ACTTTCAAAT CTGGAAAATT TATAAGAATT TTTCAGCTGA GCAACAACAT TAGAAGTGTG 
GTCCTTAGAC ATTGTAAAAA AAGACAAAGT CACCTGCGTT TAACTTTGAA AAACAACGTG 
TTCTTGTTTA TTGACAAATT ATCCTACAGA GATGCTAAAC AGTTGAATAT GTTCCTGGAC 
ATAATCCACC AAAACAAATC TCAGCAACCC ATGAAATCTG ATGATGATTG GAGTGTGTTT 
GAAAGCAGGA ATATGCTGAA GGAAATTGAC AAAACTTCAT TTTACAGCAT TTGTAACAAG 
CCAAGTTATC AGAAGATGCC TTTGTTTATG TCAAAATCAC CAACACATGT GAAAAAGGGG 
ATATTAGAAA ATCAAGGTGG GAAGGGGCAA AACACACTAT CATCTGATGT ACAGACAAAT 
GAGGACATTC TGAAGGAAGA TAACCCTGTA CCAAACAAGA AATATAAGAC AGATTCCTTG 
AAATATATAC AAAGCAATAG GAAGAACCCA TCAAGTTTAG AGGATTTAGA AAAAGATAGA 
GATTTGAAAC TCGGGCCTTC ATTCAATACC AACTGTAATG GAAATCCTAA CCTAGATGAG 
ACTGTTCTTG CAACCCAGAC TCTCAATGCC AAAAATGGTT TGACATCTCC ATTGGAACCA 
GAGCACAGCC AGGGTGACCC AAGATGCAAC AAAGCCCAGG TGCCTCTTGA CTCTCATTCA 
CAGCAACTGC AGCAGGGGTT CCCCAATTTG GGAAACACCT GTTACATGAA TGCAGTTTTA 
CAATCGCTAT TTGCAATTCC ATCTTTTGCT GATGACTTAC TCACTCAAGG TGTCCCATGG 
GAATATATTC CCTTTGAGGC TCTTATTATG ACCTTGACCC AGCTGCTTGC TTTGAAAGAT 
TTCTGTAGTA CAAAGATCAA GAGAGAATTA CTTGGGAATG TTAAAAAAGT CATTTCAGCA 
GTTGCAGAAA TATTTTCTGG CAACATGCAG AATGATGCTC ATGAGTTTTT AGGTCAGTGT 
TTAGACCAGC TGAAAGAAGA CATGGAAAAA TTAAATGCCA CTTTGAATAC TGGGAAAGAA 
TGTGGGGATG AAAATTCATC TCCACAAATG CATGTTGGTA GTGCTGCCAC CAAAGTGTTT 
GTTTGCCCTG TTGTTGCTAA TTTTGAGTTT GAATTGCAGC TCTCCCTTAT TTGTAAAGCT 
TGTGGTCATG CTGTTCTCAA GGTAGAACCT AATAATTATC TCTCCATCAA CCTGCACCAA 
GAAACAAAAC CACTTCCTTT GTCCATTCAG AATTCTTTAG ATCTTTTCTT TAAAGAAGAA 
GAGCTTGAAT ATAACTGTCA GATGTGTAAG CAGAAGAGTT GTGTTGCAAG GCATACATTT 
AGTAGGCTCT CCAGGGTCCT TATCATTCAT CTGAAACGCT ATAGCTTCAA CAATGCTTGG 
TTGCTGGTGA AGAATAACGA GCAAGTTTAT ATTCCCAAAT CTTTAAGTTT ATCTTCTTAT 
TGCAATGAAA GCACCAAACC ACCTCTTCCC TTGAGCAGTA GTGCACCTGT TGGGAAATGT 
GAAGTCCTGG AAGTCTCTCA GGAGATGATT TCTGAGATCA ACAGCCCATT GACACCATCA 
ATGAAGCTGA CCTCAGAATC CAGTGATTCC CTGGTTCTAC CCGTTGAACC AGACAAGAAT 
GCCGACCTAC AAAGATTCCA GAGAGACTGT GGAGATGCAA GCCAAGAGCA GCATCAGAGA 
GACCTGGAAA ATGGCTCTGC ACTAGAGTCA GAATTGGTCC ACTTTAGAGA TAGGGCAATC 
GGTGAAAAGG AGCTTCCAGT GGCTGACTCA CTGATGGACC AGGGAGACAT TTCTCTTCCT 
GTGATGTATG AAGATGGAGG GAAGCTGATC AGCAGCCCAG ACACAAGGCT TGTCGAGGTT 
CATCTTCAAG AGGTGCCTCA ACATCCAGAA CTTCAGAAGT ATGAGAAAAC CAATACATTC 
GTAGAGTTCA ATTTTGACAG TGTCACTGAG TCCACCAATG GCTTTTATGA CTGTAAAGAA 
AACAGGATTC CAGAAGGATC TCAAGGAATG GCTGAACAGC TCCAGCAGTG TATTGAGGAG 
AGCATCATAG ATGAATTTCT TCAGCAGGCA CCACCTCCAG GTGTTAGGAA GCTGGATGCC 
CAGGAACATA CAGAAGAGAC CCTCAATCAG TCTACAGAAT TAAGACTTCA AAAGGCTGAC 
CTGAATCACC TTGGGGCACT GGGTTCTGAC AACCCAGGAA ACAAAAACAT TTTAGATGCA 
GAGAACACAA GAGGTGAAGC CAAGGAACTA ACAAGAAACG TGAAGATGGG GGATCCTCTC 
CAGGCCTACA GACTCATCAG TGTTGTCAGC CATATCGGGA GCTCCCCAAA TTCAGGCCAT 
TACATCAGCG ATGTGTATGA CTTTCAGAAG CAGGCCTGGT TCACATACAA CGATCTATGT 
GTATCAGAAA TCTCAGAGAC CAAAATGCAG GAGGCGAGGC TTCACTCTGG GTATATCTTC 
TTTTACATGC ACAATGGGAT TTTTGAGGAG CTGTTAAGAA AAGCAGAGAA CTCTCGGCTA 
CCTAGCACAC AGGCAGGGGT GATCCCTCAG GGGGAATACG AAGGTGACTC TTTGTACAGA 
CCTGCTTGA