THAP11 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	THAP11
Entrez Gene ID	57215
Full Name	THAP domain containing 11
Synonyms	CTG-B43a,CTG-B45d,HRIHFB2206,RONIN
General protein information	Preferred Names THAP domain containing 11 Names THAP domain-containing protein 11
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q22.1
Summary	The protein encoded by this gene contains a THAP domain, which is a conserved DNA-binding domain that has striking similarity to the site-specific DNA-binding domain (DBD) of Drosophila P element transposases. [provided by RefSeq, Jul 2008].
Disorder MIM:	609119

mRNA and Protein(s)

mRNA	Protein	Name
NM_020457.3	NP_065190.2	THAP domain-containing protein 11
NM_020457.2	NP_065190.2	THAP domain-containing protein 11

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Thap11	NP_001100892.1
Pan troglodytes (chimpanzee)	THAP11	XP_511041.1
Bos taurus (cattle)	THAP11	NP_001098464.1
Mus musculus (house mouse)	Thap11	NP_067488.1
Danio rerio (zebrafish)	thap11	NP_998267.1
Homo sapiens (human)	THAP11	NP_065190.2
	THAP11	XP_001095010.1
Canis lupus familiaris (dog)	THAP11	XP_005620865.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide CRISPR screen for PARKIN regulators reveals transcriptional repression as a determinant of mitophagy.
Potting C, Crochemore C, Moretti F, Nigsch F, Schmidt I, Manneville C, Carbone W, Knehr J, DeJesus R, Lindeman A, Maher R, Russ C, McAllister G, Reece-Hoyes JS, Hoffman GR, Roma G, M?ller M, Sailer AW, Helliwell SB
Proceedings of the National Academy of Sciences of the United States of America115(2)E180-E189(2018 Jan)

Mutations in THAP11 cause an inborn error of cobalamin metabolism and developmental abnormalities.
Quintana AM, Yu HC, Brebner A, Pupavac M, Geiger EA, Watson A, Castro VL, Cheung W, Chen SH, Watkins D, Pastinen T, Skovby F, Appel B, Rosenblatt DS, Shaikh TH
Human molecular genetics26(15)2838-2849(2017 Aug)

Biochemical and cellular characterization of transcription factors binding to the hyperconserved core promoter-associated M4 motif.
Trung NT, Kremmer E, Mittler G
BMC genomics17693(2016 08)

The C-terminal region of the transcriptional regulator THAP11 forms a parallel coiled-coil domain involved in protein dimerization.
Cukier CD, Maveyraud L, Saurel O, Guillet V, Milon A, Gervais V
Journal of structural biology194(3)337-46(2016 Jun)

Host cell factor-1 recruitment to E2F-bound and cell-cycle-control genes is mediated by THAP11 and ZNF143.
Parker JB, Yin H, Vinckevicius A, Chakravarti D
Cell reports9(3)967-82(2014 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We sequenced THAP11 by Sanger sequencing and discovered a potentially pathogenic, homozygous variant in THAP11, c.240C > G (p.Phe80Leu). Further, we provide functional data in model organisms that suggests that both HCFC1 and THAP11 are essential for normal brain development and neural precursor differentiation.
Title: Mutations in THAP11 cause an inborn error of cobalamin metabolism and developmental abnormalities.

The M4 motif (ACTAYRNNNCCCR) is a functional regulatory bipartite cis-element, which engages a THAP11/HCF-1 complex via binding to the ACTAYR module, while the CCCRRNRNRC subsequence part constitutes a binding platform for Ikaros and NFKB1
Title: Biochemical and cellular characterization of transcription factors binding to the hyperconserved core promoter-associated M4 motif.

the crystal structure of the C-ter region of THAP11 forms a left-handed parallel homo-dimeric coiled-coil structure possessing several unusual features.
Title: The C-terminal region of the transcriptional regulator THAP11 forms a parallel coiled-coil domain involved in protein dimerization.

THAP11, ZNF143, and HCF-1 form a mutually dependent complex on chromatin, which is independent of E2F occupancy.
Title: Host cell factor-1 recruitment to E2F-bound and cell-cycle-control genes is mediated by THAP11 and ZNF143.

that THAP11 reversibly regulated erythroid and megakaryocytic differentiation.
Title: Effects of THAP11 on erythroid differentiation and megakaryocytic differentiation of K562 cells.

The following THAP11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THAP11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31301	NM_020457.3 Latest version!	Homo sapiens THAP domain containing 11 (THAP11), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
View Old Accession Versions >>
OHu31301	NM_020457.2	Homo sapiens THAP domain containing 11 (THAP11), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

THAP11 ( NM_020457.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065190.2 Homo sapiens THAP domain containing 11 (THAP11), mRNA.

Price & Availability↑

CloneID	OHu31301
Clone ID Related Accession (Same CDS sequence)	NM_020457.3 , NM_020457.2
Accession Version	NM_020457.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	THAP domain-containing protein 11
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ898449.1, BI261822.1 and BC012182.1. On May 17, 2019 this sequence version replaced NM_020457.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC012182.1, SRR1163655.131044.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000303596.3/ ENSP00000304689.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCTGGCT TTACGTGCTG CGTGCCAGGC TGCTACAACA ACTCGCACCG GGACAAGGCG 
CTGCACTTCT ACACGTTTCC AAAGGACGCT GAGTTGCGGC GCCTCTGGCT CAAGAACGTG 
TCGCGTGCCG GCGTCAGTGG GTGCTTCTCC ACCTTCCAGC CCACCACAGG CCACCGTCTC 
TGCAGCGTTC ACTTCCAGGG CGGCCGCAAG ACCTACACGG TACGCGTCCC CACCATCTTC 
CCGCTGCGCG GCGTCAATGA GCGCAAAGTA GCGCGCAGAC CCGCTGGGGC CGCGGCCGCC 
CGCCGCAGGC AGCAGCAGCA ACAGCAGCAG CAGCAGCAAC AGCAGCAACA GCAGCAGCAG 
CAGCAACAGC AGCAGCAGCA GCAGCAGCAG CAGCAGTCCT CACCCTCTGC CTCCACTGCC 
CAGACTGCCC AGCTGCAGCC GAACCTGGTA TCTGCTTCCG CGGCCGTGCT TCTCACCCTT 
CAGGCCACTG TAGACAGCAG TCAGGCTCCG GGATCCGTAC AGCCGGCGCC CATCACTCCC 
ACTGGAGAAG ACGTGAAGCC CATCGATCTC ACAGTGCAAG TGGAGTTTGC AGCCGCAGAG 
GGCGCAGCCG CTGCGGCCGC CGCGTCGGAG TTACAGGCTG CTACCGCAGG GCTGGAGGCT 
GCCGAGTGCC CTATGGGCCC CCAGTTGGTG GTGGTAGGGG AAGAGGGCTT CCCTGATACT 
GGCTCCGACC ATTCGTACTC CTTGTCGTCA GGCACCACGG AGGAGGAGCT CCTGCGCAAG 
CTGAATGAGC AGCGGGACAT CCTGGCTCTG ATGGAAGTGA AGATGAAAGA GATGAAAGGC 
AGCATTCGCC ACCTGCGTCT CACTGAGGCC AAGCTGCGCG AAGAACTGCG TGAGAAGGAT 
CGGCTGCTTG CCATGGCTGT CATCCGCAAG AAGCACGGAA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065190.2
CDS	236..1180
Translation	MPGFTCCVPGCYNNSHRDKALHFYTFPKDAELRRLWLKNVSRAGVSGCFSTFQPTTGHRLCSVHFQGGRKTYTVRVPTIFPLRGVNERKVARRPAGAAAARRRQQQQQQQQQQQQQQQQQQQQQQQQQQQQQSSPSASTAQTAQLQPNLVSASAAVLLTLQATVDSSQAPGSVQPAPITPTGEDVKPIDLTVQVEFAAAEGAAAAAAASELQAATAGLEAAECPMGPQLVVVGEEGFPDTGSDHSYSLSSGTTEEELLRKLNEQRDILALMEVKMKEMKGSIRHLRLTEAKLREELREKDRLLAMAVIRKKHGM

Target ORF information:

RefSeq Version	NM_020457.3
Organism	Homo sapiens(human)
Definition	Homo sapiens THAP domain containing 11 (THAP11), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020457.3

ORF Insert Sequence:

ATGCCTGGCT TTACGTGCTG CGTGCCAGGC TGCTACAACA ACTCGCACCG GGACAAGGCG 
CTGCACTTCT ACACGTTTCC AAAGGACGCT GAGTTGCGGC GCCTCTGGCT CAAGAACGTG 
TCGCGTGCCG GCGTCAGTGG GTGCTTCTCC ACCTTCCAGC CCACCACAGG CCACCGTCTC 
TGCAGCGTTC ACTTCCAGGG CGGCCGCAAG ACCTACACGG TACGCGTCCC CACCATCTTC 
CCGCTGCGCG GCGTCAATGA GCGCAAAGTA GCGCGCAGAC CCGCTGGGGC CGCGGCCGCC 
CGCCGCAGGC AGCAGCAGCA ACAGCAGCAG CAGCAGCAAC AGCAGCAACA GCAGCAGCAG 
CAGCAACAGC AGCAGCAGCA GCAGCAGCAG CAGCAGTCCT CACCCTCTGC CTCCACTGCC 
CAGACTGCCC AGCTGCAGCC GAACCTGGTA TCTGCTTCCG CGGCCGTGCT TCTCACCCTT 
CAGGCCACTG TAGACAGCAG TCAGGCTCCG GGATCCGTAC AGCCGGCGCC CATCACTCCC 
ACTGGAGAAG ACGTGAAGCC CATCGATCTC ACAGTGCAAG TGGAGTTTGC AGCCGCAGAG 
GGCGCAGCCG CTGCGGCCGC CGCGTCGGAG TTACAGGCTG CTACCGCAGG GCTGGAGGCT 
GCCGAGTGCC CTATGGGCCC CCAGTTGGTG GTGGTAGGGG AAGAGGGCTT CCCTGATACT 
GGCTCCGACC ATTCGTACTC CTTGTCGTCA GGCACCACGG AGGAGGAGCT CCTGCGCAAG 
CTGAATGAGC AGCGGGACAT CCTGGCTCTG ATGGAAGTGA AGATGAAAGA GATGAAAGGC 
AGCATTCGCC ACCTGCGTCT CACTGAGGCC AAGCTGCGCG AAGAACTGCG TGAGAAGGAT 
CGGCTGCTTG CCATGGCTGT CATCCGCAAG AAGCACGGAA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THAP11 ( NM_020457.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_065190.2 Homo sapiens THAP domain containing 11 (THAP11), mRNA.

Price & Availability↑

CloneID	OHu31301
Clone ID Related Accession (Same CDS sequence)	NM_020457.3 , NM_020457.2
Accession Version	NM_020457.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	THAP domain-containing protein 11
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ898449.1, BI261822.1 and BC012182.1. On Dec 24, 2003 this sequence version replaced NM_020457.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

ATGCCTGGCT TTACGTGCTG CGTGCCAGGC TGCTACAACA ACTCGCACCG GGACAAGGCG 
CTGCACTTCT ACACGTTTCC AAAGGACGCT GAGTTGCGGC GCCTCTGGCT CAAGAACGTG 
TCGCGTGCCG GCGTCAGTGG GTGCTTCTCC ACCTTCCAGC CCACCACAGG CCACCGTCTC 
TGCAGCGTTC ACTTCCAGGG CGGCCGCAAG ACCTACACGG TACGCGTCCC CACCATCTTC 
CCGCTGCGCG GCGTCAATGA GCGCAAAGTA GCGCGCAGAC CCGCTGGGGC CGCGGCCGCC 
CGCCGCAGGC AGCAGCAGCA ACAGCAGCAG CAGCAGCAAC AGCAGCAACA GCAGCAGCAG 
CAGCAACAGC AGCAGCAGCA GCAGCAGCAG CAGCAGTCCT CACCCTCTGC CTCCACTGCC 
CAGACTGCCC AGCTGCAGCC GAACCTGGTA TCTGCTTCCG CGGCCGTGCT TCTCACCCTT 
CAGGCCACTG TAGACAGCAG TCAGGCTCCG GGATCCGTAC AGCCGGCGCC CATCACTCCC 
ACTGGAGAAG ACGTGAAGCC CATCGATCTC ACAGTGCAAG TGGAGTTTGC AGCCGCAGAG 
GGCGCAGCCG CTGCGGCCGC CGCGTCGGAG TTACAGGCTG CTACCGCAGG GCTGGAGGCT 
GCCGAGTGCC CTATGGGCCC CCAGTTGGTG GTGGTAGGGG AAGAGGGCTT CCCTGATACT 
GGCTCCGACC ATTCGTACTC CTTGTCGTCA GGCACCACGG AGGAGGAGCT CCTGCGCAAG 
CTGAATGAGC AGCGGGACAT CCTGGCTCTG ATGGAAGTGA AGATGAAAGA GATGAAAGGC 
AGCATTCGCC ACCTGCGTCT CACTGAGGCC AAGCTGCGCG AAGAACTGCG TGAGAAGGAT 
CGGCTGCTTG CCATGGCTGT CATCCGCAAG AAGCACGGAA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065190.2
CDS	246..1190
Translation	MPGFTCCVPGCYNNSHRDKALHFYTFPKDAELRRLWLKNVSRAGVSGCFSTFQPTTGHRLCSVHFQGGRKTYTVRVPTIFPLRGVNERKVARRPAGAAAARRRQQQQQQQQQQQQQQQQQQQQQQQQQQQQQSSPSASTAQTAQLQPNLVSASAAVLLTLQATVDSSQAPGSVQPAPITPTGEDVKPIDLTVQVEFAAAEGAAAAAAASELQAATAGLEAAECPMGPQLVVVGEEGFPDTGSDHSYSLSSGTTEEELLRKLNEQRDILALMEVKMKEMKGSIRHLRLTEAKLREELREKDRLLAMAVIRKKHGM

Target ORF information:

RefSeq Version	NM_020457.2
Organism	Homo sapiens(human)
Definition	Homo sapiens THAP domain containing 11 (THAP11), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020457.2

ORF Insert Sequence:

ATGCCTGGCT TTACGTGCTG CGTGCCAGGC TGCTACAACA ACTCGCACCG GGACAAGGCG 
CTGCACTTCT ACACGTTTCC AAAGGACGCT GAGTTGCGGC GCCTCTGGCT CAAGAACGTG 
TCGCGTGCCG GCGTCAGTGG GTGCTTCTCC ACCTTCCAGC CCACCACAGG CCACCGTCTC 
TGCAGCGTTC ACTTCCAGGG CGGCCGCAAG ACCTACACGG TACGCGTCCC CACCATCTTC 
CCGCTGCGCG GCGTCAATGA GCGCAAAGTA GCGCGCAGAC CCGCTGGGGC CGCGGCCGCC 
CGCCGCAGGC AGCAGCAGCA ACAGCAGCAG CAGCAGCAAC AGCAGCAACA GCAGCAGCAG 
CAGCAACAGC AGCAGCAGCA GCAGCAGCAG CAGCAGTCCT CACCCTCTGC CTCCACTGCC 
CAGACTGCCC AGCTGCAGCC GAACCTGGTA TCTGCTTCCG CGGCCGTGCT TCTCACCCTT 
CAGGCCACTG TAGACAGCAG TCAGGCTCCG GGATCCGTAC AGCCGGCGCC CATCACTCCC 
ACTGGAGAAG ACGTGAAGCC CATCGATCTC ACAGTGCAAG TGGAGTTTGC AGCCGCAGAG 
GGCGCAGCCG CTGCGGCCGC CGCGTCGGAG TTACAGGCTG CTACCGCAGG GCTGGAGGCT 
GCCGAGTGCC CTATGGGCCC CCAGTTGGTG GTGGTAGGGG AAGAGGGCTT CCCTGATACT 
GGCTCCGACC ATTCGTACTC CTTGTCGTCA GGCACCACGG AGGAGGAGCT CCTGCGCAAG 
CTGAATGAGC AGCGGGACAT CCTGGCTCTG ATGGAAGTGA AGATGAAAGA GATGAAAGGC 
AGCATTCGCC ACCTGCGTCT CACTGAGGCC AAGCTGCGCG AAGAACTGCG TGAGAAGGAT 
CGGCTGCTTG CCATGGCTGT CATCCGCAAG AAGCACGGAA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide CRISPR screen for PARKIN regulators reveals transcriptional repression as a determinant of mitophagy.
Proceedings of the National Academy of Sciences of the United States of America115(2)E180-E189(2018 Jan)
Potting C,Crochemore C,Moretti F,Nigsch F,Schmidt I,Manneville C,Carbone W,Knehr J,DeJesus R,Lindeman A,Maher R,Russ C,McAllister G,Reece-Hoyes JS,Hoffman GR,Roma G,M?ller M,Sailer AW,Helliwell SB

Mutations in THAP11 cause an inborn error of cobalamin metabolism and developmental abnormalities.
Human molecular genetics26(15)2838-2849(2017 Aug)
Quintana AM,Yu HC,Brebner A,Pupavac M,Geiger EA,Watson A,Castro VL,Cheung W,Chen SH,Watkins D,Pastinen T,Skovby F,Appel B,Rosenblatt DS,Shaikh TH

Biochemical and cellular characterization of transcription factors binding to the hyperconserved core promoter-associated M4 motif.
BMC genomics17693(2016 08)
Trung NT,Kremmer E,Mittler G

The C-terminal region of the transcriptional regulator THAP11 forms a parallel coiled-coil domain involved in protein dimerization.
Journal of structural biology194(3)337-46(2016 Jun)
Cukier CD,Maveyraud L,Saurel O,Guillet V,Milon A,Gervais V

Host cell factor-1 recruitment to E2F-bound and cell-cycle-control genes is mediated by THAP11 and ZNF143.
Cell reports9(3)967-82(2014 Nov)
Parker JB,Yin H,Vinckevicius A,Chakravarti D