NT5C cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NT5C
Entrez Gene ID	30833
Full Name	5', 3'-nucleotidase, cytosolic
Synonyms	DNT,DNT1,HEL74,P5N2,PN-I,PN-II,UMPH2,cdN,dNT-1
General protein information	Preferred Names 5', 3'-nucleotidase, cytosolic Names 5'(3')-deoxyribonucleotidase, cytosolic type 5' nucleotidase, deoxy (pyrimidine), cytosolic type C cytosolic 5',3'-pyrimidine nucleotidase deoxy-5'-nucleotidase 1 epididymis luminal protein 74 uridine 5'-monophosphate phosphohydrolase 2 uridine 5-prime monophosphate hydrolase 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q25.1
Summary	This gene encodes a nucleotidase that catalyzes the dephosphorylation of the 5' deoxyribonucleotides (dNTP) and 2'(3')-dNTP and ribonucleotides, but not 5' ribonucleotides. Of the different forms of nucleotidases characterized, this enzyme is unique in its preference for 5'-dNTP. It may be one of the enzymes involved in regulating the size of dNTP pools in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2011].
Disorder MIM:	191720

mRNA and Protein(s)

mRNA	Protein	Name
NM_001252377.1	NP_001239306.1	5'(3')-deoxyribonucleotidase, cytosolic type isoform 2
XM_011524700.2	XP_011523002.2	5'(3')-deoxyribonucleotidase, cytosolic type isoform X1
NM_014595.2	NP_055410.1	5'(3')-deoxyribonucleotidase, cytosolic type isoform 1
NM_014595.3	NP_055410.1	5'(3')-deoxyribonucleotidase, cytosolic type isoform 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Nt5c	NP_001258118.1
	NT5C	XP_001093693.2
Bos taurus (cattle)	NT5C	NP_001039787.1
Pan troglodytes (chimpanzee)	NT5C	XP_001139013.1
Canis lupus familiaris (dog)	NT5C	XP_540428.3
Mus musculus (house mouse)	Nt5c	NP_056622.1
Homo sapiens (human)	NT5C	NP_055410.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

5'-(3')-nucleotidase mRNA levels in blast cells are a prognostic factor in acute myeloid leukemia patients treated with cytarabine.
Galmarini CM, Cros E, Graham K, Thomas X, Mackey JR, Dumontet C
Haematologica89(5)617-9(2004 May)

Molecular characterization of Turkish patients with pyrimidine 5' nucleotidase-I deficiency.
Balta G, Gumruk F, Akarsu N, Gurgey A, Altay C
Blood102(5)1900-3(2003 Sep)

Kinetic evidence for covalent phosphoryl-enzyme intermediate in phosphotransferase activity of human red cell pyrimidine nucleotidases.
Amici A, Emanuelli M, Ruggieri S, Raffaelli N, Magni G
Methods in enzymology354149-59(2002)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Mutation analysis in the P5N-I gene led to the identification of 3 novel mutations
Title: Molecular characterization of Turkish patients with pyrimidine 5' nucleotidase-I deficiency.

Data show that PN-I shows a higher affinity for oxynucleosides rather than deoxynucleosides, while the opposite is true for PN-II.
Title: Kinetic evidence for covalent phosphoryl-enzyme intermediate in phosphotransferase activity of human red cell pyrimidine nucleotidases.

The following NT5C gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NT5C cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09591	NM_014595.3 Latest version!	Homo sapiens 5', 3'-nucleotidase, cytosolic (NT5C), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu10084	NM_001252377.1 Latest version!	Homo sapiens 5', 3'-nucleotidase, cytosolic (NT5C), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu86812	XM_011524700.2 Latest version!	Homo sapiens 5', 3'-nucleotidase, cytosolic (NT5C), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
View Old Accession Versions >>
OHu09591	NM_014595.2	Homo sapiens 5', 3'-nucleotidase, cytosolic (NT5C), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NT5C ( NM_014595.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_055410.1 Homo sapiens 5', 3'-nucleotidase, cytosolic (NT5C), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09591
Clone ID Related Accession (Same CDS sequence)	NM_014595.2 , NM_014595.3
Accession Version	NM_014595.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	5'(3')-deoxyribonucleotidase, cytosolic type isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ431723.1, AF154829.2 and BU627693.1. On Nov 11, 2011 this sequence version replaced NM_014595.1. Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.16179.1, AF154829.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCGGA GCGTGCGCGT GCTGGTGGAC ATGGACGGCG TCCTGGCCGA CTTCGAGGCC 
GGCCTCCTGC GGGGCTTCCG CCGCCGCTTC CCTGAGGAGC CGCACGTGCC GCTGGAGCAA 
CGCCGCGGCT TCCTGGCCCG CGAGCAGTAC CGCGCCCTGC GGCCCGACCT GGCGGATAAA 
GTGGCCAGTG TGTACGAAGC CCCGGGCTTT TTCCTGGACC TGGAGCCCAT CCCGGGAGCC 
TTGGACGCTG TGCGGGAGAT GAACGACCTA CCGGACACGC AGGTCTTCAT CTGCACCAGC 
CCCCTGCTGA AGTACCACCA CTGTGTGGGT GAGAAGTACC GCTGGGTGGA GCAGCACCTG 
GGGCCCCAGT TCGTAGAACG AATTATCCTG ACAAGGGACA AGACGGTGGT CTTGGGGGAC 
CTGCTCATTG ATGACAAGGA CACAGTTCGA GGCCAGGAGG AGACCCCAAG CTGGGAGCAC 
ATCTTGTTCA CCTGCTGCCA CAATCGGCAC CTGGTCCTGC CCCCGACAAG GAGACGGCTG 
CTCTCCTGGA GTGACAACTG GAGGGAGATC TTAGATAGCA AGCGCGGAGC TGCGCAGCGG 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055410.1
CDS	89..694
Translation	MARSVRVLVDMDGVLADFEAGLLRGFRRRFPEEPHVPLEQRRGFLAREQYRALRPDLADKVASVYEAPGFFLDLEPIPGALDAVREMNDLPDTQVFICTSPLLKYHHCVGEKYRWVEQHLGPQFVERIILTRDKTVVLGDLLIDDKDTVRGQEETPSWEHILFTCCHNRHLVLPPTRRRLLSWSDNWREILDSKRGAAQRE

Target ORF information:

RefSeq Version	NM_014595.2
Organism	Homo sapiens(human)
Definition	Homo sapiens 5', 3'-nucleotidase, cytosolic (NT5C), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014595.2

ORF Insert Sequence:

ATGGCGCGGA GCGTGCGCGT GCTGGTGGAC ATGGACGGCG TCCTGGCCGA CTTCGAGGCC 
GGCCTCCTGC GGGGCTTCCG CCGCCGCTTC CCTGAGGAGC CGCACGTGCC GCTGGAGCAA 
CGCCGCGGCT TCCTGGCCCG CGAGCAGTAC CGCGCCCTGC GGCCCGACCT GGCGGATAAA 
GTGGCCAGTG TGTACGAAGC CCCGGGCTTT TTCCTGGACC TGGAGCCCAT CCCGGGAGCC 
TTGGACGCTG TGCGGGAGAT GAACGACCTA CCGGACACGC AGGTCTTCAT CTGCACCAGC 
CCCCTGCTGA AGTACCACCA CTGTGTGGGT GAGAAGTACC GCTGGGTGGA GCAGCACCTG 
GGGCCCCAGT TCGTAGAACG AATTATCCTG ACAAGGGACA AGACGGTGGT CTTGGGGGAC 
CTGCTCATTG ATGACAAGGA CACAGTTCGA GGCCAGGAGG AGACCCCAAG CTGGGAGCAC 
ATCTTGTTCA CCTGCTGCCA CAATCGGCAC CTGGTCCTGC CCCCGACAAG GAGACGGCTG 
CTCTCCTGGA GTGACAACTG GAGGGAGATC TTAGATAGCA AGCGCGGAGC TGCGCAGCGG 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NT5C ( NM_014595.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055410.1 Homo sapiens 5', 3'-nucleotidase, cytosolic (NT5C), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09591
Clone ID Related Accession (Same CDS sequence)	NM_014595.2 , NM_014595.3
Accession Version	NM_014595.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	5'(3')-deoxyribonucleotidase, cytosolic type isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF154829.2. On Nov 23, 2018 this sequence version replaced NM_014595.2. Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC017454.1, AF154829.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000245552.7/ ENSP00000245552.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGCGGA GCGTGCGCGT GCTGGTGGAC ATGGACGGCG TCCTGGCCGA CTTCGAGGCC 
GGCCTCCTGC GGGGCTTCCG CCGCCGCTTC CCTGAGGAGC CGCACGTGCC GCTGGAGCAA 
CGCCGCGGCT TCCTGGCCCG CGAGCAGTAC CGCGCCCTGC GGCCCGACCT GGCGGATAAA 
GTGGCCAGTG TGTACGAAGC CCCGGGCTTT TTCCTGGACC TGGAGCCCAT CCCGGGAGCC 
TTGGACGCTG TGCGGGAGAT GAACGACCTA CCGGACACGC AGGTCTTCAT CTGCACCAGC 
CCCCTGCTGA AGTACCACCA CTGTGTGGGT GAGAAGTACC GCTGGGTGGA GCAGCACCTG 
GGGCCCCAGT TCGTAGAACG AATTATCCTG ACAAGGGACA AGACGGTGGT CTTGGGGGAC 
CTGCTCATTG ATGACAAGGA CACAGTTCGA GGCCAGGAGG AGACCCCAAG CTGGGAGCAC 
ATCTTGTTCA CCTGCTGCCA CAATCGGCAC CTGGTCCTGC CCCCGACAAG GAGACGGCTG 
CTCTCCTGGA GTGACAACTG GAGGGAGATC TTAGATAGCA AGCGCGGAGC TGCGCAGCGG 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055410.1
CDS	36..641
Translation	MARSVRVLVDMDGVLADFEAGLLRGFRRRFPEEPHVPLEQRRGFLAREQYRALRPDLADKVASVYEAPGFFLDLEPIPGALDAVREMNDLPDTQVFICTSPLLKYHHCVGEKYRWVEQHLGPQFVERIILTRDKTVVLGDLLIDDKDTVRGQEETPSWEHILFTCCHNRHLVLPPTRRRLLSWSDNWREILDSKRGAAQRE

Target ORF information:

RefSeq Version	NM_014595.3
Organism	Homo sapiens(human)
Definition	Homo sapiens 5', 3'-nucleotidase, cytosolic (NT5C), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014595.3

ORF Insert Sequence:

ATGGCGCGGA GCGTGCGCGT GCTGGTGGAC ATGGACGGCG TCCTGGCCGA CTTCGAGGCC 
GGCCTCCTGC GGGGCTTCCG CCGCCGCTTC CCTGAGGAGC CGCACGTGCC GCTGGAGCAA 
CGCCGCGGCT TCCTGGCCCG CGAGCAGTAC CGCGCCCTGC GGCCCGACCT GGCGGATAAA 
GTGGCCAGTG TGTACGAAGC CCCGGGCTTT TTCCTGGACC TGGAGCCCAT CCCGGGAGCC 
TTGGACGCTG TGCGGGAGAT GAACGACCTA CCGGACACGC AGGTCTTCAT CTGCACCAGC 
CCCCTGCTGA AGTACCACCA CTGTGTGGGT GAGAAGTACC GCTGGGTGGA GCAGCACCTG 
GGGCCCCAGT TCGTAGAACG AATTATCCTG ACAAGGGACA AGACGGTGGT CTTGGGGGAC 
CTGCTCATTG ATGACAAGGA CACAGTTCGA GGCCAGGAGG AGACCCCAAG CTGGGAGCAC 
ATCTTGTTCA CCTGCTGCCA CAATCGGCAC CTGGTCCTGC CCCCGACAAG GAGACGGCTG 
CTCTCCTGGA GTGACAACTG GAGGGAGATC TTAGATAGCA AGCGCGGAGC TGCGCAGCGG 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NT5C ( NM_001252377.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001239306.1 Homo sapiens 5', 3'-nucleotidase, cytosolic (NT5C), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10084
Clone ID Related Accession (Same CDS sequence)	NM_001252377.1
Accession Version	NM_001252377.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	5'(3')-deoxyribonucleotidase, cytosolic type isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ431723.1, AF154829.2, BG034032.1 and BU627693.1. Transcript Variant: This variant (2) lacks an in-frame coding exon, and uses an alternate in-frame acceptor splice site at the 3' terminal exon compared to variant 1. This results in a shorter isoform (2) missing an internal protein segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BG034032.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCGGA GCGTGCGCGT GCTGGTGGAC ATGGACGGCG TCCTGGCCGA CTTCGAGGCC 
GGCCTCCTGC GGGGCTTCCG CCGCCGCTTC CCTGAGGAGC CGCACGTGCC GCTGGAGCAA 
CGCCGCGGCT TCCTGGCCCG CGAGCAGTAC CGCGCCCTGC GGCCCGACCT GGCGGATAAA 
GTGGCCAGTG TGTACGAAGC CCCGGGCTTT TTCCTGGACC TGGAGCCCAT CCCGGGAGCC 
TTGGACGCTG TGCGGGAGAT GAACGACCTA CCGGACACGC AGGTCTTCAT CTGCACCAGC 
CCCCTGCTGA AGTACCACCA CTGTGTGGGT GAGAAGGAGG AGACCCCAAG CTGGGAGCAC 
ATCTTGTTCA CCTGCTGCCA CAATCGGCAC CTGGTCCTGC CCCCGACAAG GAGACGGCTG 
CTCTCCTGGA GTGACAACTG GAGGGAGATC TTAGATAGCA AGCGCGGAGC TGCGCAGCGG 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239306.1
CDS	89..574
Translation	MARSVRVLVDMDGVLADFEAGLLRGFRRRFPEEPHVPLEQRRGFLAREQYRALRPDLADKVASVYEAPGFFLDLEPIPGALDAVREMNDLPDTQVFICTSPLLKYHHCVGEKEETPSWEHILFTCCHNRHLVLPPTRRRLLSWSDNWREILDSKRGAAQRE

Target ORF information:

RefSeq Version	NM_001252377.1
Organism	Homo sapiens(human)
Definition	Homo sapiens 5', 3'-nucleotidase, cytosolic (NT5C), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252377.1

ORF Insert Sequence:

ATGGCGCGGA GCGTGCGCGT GCTGGTGGAC ATGGACGGCG TCCTGGCCGA CTTCGAGGCC 
GGCCTCCTGC GGGGCTTCCG CCGCCGCTTC CCTGAGGAGC CGCACGTGCC GCTGGAGCAA 
CGCCGCGGCT TCCTGGCCCG CGAGCAGTAC CGCGCCCTGC GGCCCGACCT GGCGGATAAA 
GTGGCCAGTG TGTACGAAGC CCCGGGCTTT TTCCTGGACC TGGAGCCCAT CCCGGGAGCC 
TTGGACGCTG TGCGGGAGAT GAACGACCTA CCGGACACGC AGGTCTTCAT CTGCACCAGC 
CCCCTGCTGA AGTACCACCA CTGTGTGGGT GAGAAGGAGG AGACCCCAAG CTGGGAGCAC 
ATCTTGTTCA CCTGCTGCCA CAATCGGCAC CTGGTCCTGC CCCCGACAAG GAGACGGCTG 
CTCTCCTGGA GTGACAACTG GAGGGAGATC TTAGATAGCA AGCGCGGAGC TGCGCAGCGG 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NT5C ( XM_011524700.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523002.2 Homo sapiens 5', 3'-nucleotidase, cytosolic (NT5C), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu86812
Clone ID Related Accession (Same CDS sequence)	XM_011524700.2
Accession Version	XM_011524700.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	5'(3')-deoxyribonucleotidase, cytosolic type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524700.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAATCA GCCCGGCGGA CTCCGGAAGC AGCGCTCCCT CCGCTTCCGG CCGAGCCCGC 
GCCCCCCAGA CCCCGAGAGC TCGCAGCTCC GGCCCGGCGG CGATGGCGCG GAGCGTGCGC 
GTGCTGGTGG ACATGGACGG CGTCCTGGCC GACTTCGAGG CCGGCCTCCT GCGGGGCTTC 
CGCCGCCGCT TCCCTGAGGA GCCGCACGTG CCGCTGGAGC AACGCCGCGG CTTCCTGGCC 
CGCGAGCAGT ACCGCGCCCT GCGGCCCGAC CTGGCGGATA AAGTGGCCAG TGTGTACGAA 
GCCCCGGGCT TTTTCCTGGA CCTGGAGCCC ATCCCGGGAG CCTTGGACGC TGTGCGGGAG 
ATGAACGACC TACCGGATAC CGCTGGGTGG AGCAGCACCT GGGGCCCCAG TTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523002.2
CDS	157..573
Translation	MQISPADSGSSAPSASGRARAPQTPRARSSGPAAMARSVRVLVDMDGVLADFEAGLLRGFRRRFPEEPHVPLEQRRGFLAREQYRALRPDLADKVASVYEAPGFFLDLEPIPGALDAVREMNDLPDTAGWSSTWGPSS

Target ORF information:

RefSeq Version	XM_011524700.2
Organism	Homo sapiens(human)
Definition	Homo sapiens 5', 3'-nucleotidase, cytosolic (NT5C), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011524700.2

ORF Insert Sequence:

ATGCAAATCA GCCCGGCGGA CTCCGGAAGC AGCGCTCCCT CCGCTTCCGG CCGAGCCCGC 
GCCCCCCAGA CCCCGAGAGC TCGCAGCTCC GGCCCGGCGG CGATGGCGCG GAGCGTGCGC 
GTGCTGGTGG ACATGGACGG CGTCCTGGCC GACTTCGAGG CCGGCCTCCT GCGGGGCTTC 
CGCCGCCGCT TCCCTGAGGA GCCGCACGTG CCGCTGGAGC AACGCCGCGG CTTCCTGGCC 
CGCGAGCAGT ACCGCGCCCT GCGGCCCGAC CTGGCGGATA AAGTGGCCAG TGTGTACGAA 
GCCCCGGGCT TTTTCCTGGA CCTGGAGCCC ATCCCGGGAG CCTTGGACGC TGTGCGGGAG 
ATGAACGACC TACCGGATAC CGCTGGGTGG AGCAGCACCT GGGGCCCCAG TTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

5'-(3')-nucleotidase mRNA levels in blast cells are a prognostic factor in acute myeloid leukemia patients treated with cytarabine.
Haematologica89(5)617-9(2004 May)
Galmarini CM,Cros E,Graham K,Thomas X,Mackey JR,Dumontet C

Molecular characterization of Turkish patients with pyrimidine 5' nucleotidase-I deficiency.
Blood102(5)1900-3(2003 Sep)
Balta G,Gumruk F,Akarsu N,Gurgey A,Altay C

Kinetic evidence for covalent phosphoryl-enzyme intermediate in phosphotransferase activity of human red cell pyrimidine nucleotidases.
Methods in enzymology354149-59(2002)
Amici A,Emanuelli M,Ruggieri S,Raffaelli N,Magni G