Nt5c cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nt5c
Entrez Gene ID	100360582
Full Name	5', 3'-nucleotidase, cytosolic
Synonyms	Umph2
General protein information	Preferred Names 5', 3'-nucleotidase, cytosolic Names 5'(3')-deoxyribonucleotidase, cytosolic type 5,3-nucleotidase, cytosolic uridine 5-monophosphate phosphohydrolase 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001271189.1	NP_001258118.1	5'(3')-deoxyribonucleotidase, cytosolic type

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Nt5c	NP_001258118.1
	NT5C	XP_001093693.2
Bos taurus (cattle)	NT5C	NP_001039787.1
Pan troglodytes (chimpanzee)	NT5C	XP_001139013.1
Canis lupus familiaris (dog)	NT5C	XP_540428.3
Mus musculus (house mouse)	Nt5c	NP_056622.1
Homo sapiens (human)	NT5C	NP_055410.1

Gene Ontology
Bibliography

Related articles in PubMed

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

Mouse cytosolic and mitochondrial deoxyribonucleotidases: cDNA cloning of the mitochondrial enzyme, gene structures, chromosomal mapping and comparison with the human orthologs.
Rampazzo C, Kost-Alimova M, Ruzzenente B, Dumanski JP, Bianchi V
Gene294(1-2)109-17(2002 Jul)

Mammalian 5'(3')-deoxyribonucleotidase, cDNA cloning, and overexpression of the enzyme in Escherichia coli and mammalian cells.
Rampazzo C, Johansson M, Gallinaro L, Ferraro P, Hellman U, Karlsson A, Reichard P, Bianchi V
The Journal of biological chemistry275(8)5409-15(2000 Feb)

Chromosomal assignments of 23 biochemical loci of the rat by using rat x mouse somatic cell hybrids.
Yasue M, Serikawa T, Yamada J
Cytogenetics and cell genetics57(2-3)142-8(1991)

Cytoplasmic 5'(3')-nucleotidase from human placenta.
H?glund L, Reichard P
The Journal of biological chemistry265(12)6589-95(1990 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nt5c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nt5c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13665	NM_001271189.1 Latest version!	Rattus norvegicus 5', 3'-nucleotidase, cytosolic (Nt5c), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nt5c ( NM_001271189.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001258118.1 Rattus norvegicus 5', 3'-nucleotidase, cytosolic (Nt5c), mRNA.

Price & Availability↑

CloneID	ORa13665
Clone ID Related Accession (Same CDS sequence)	NM_001271189.1
Accession Version	NM_001271189.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Rattus norvegicus(Norway rat)
Product	5'(3')-deoxyribonucleotidase, cytosolic type
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FQ233678.1. On or before Sep 7, 2012 this sequence version replaced XM_003750961.1, XM_003752395.1. ##Evidence-Data-START## Transcript exon combination :: FQ233678.1, FQ227491.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGTGA AGCGGCCGGT GCGCGTGCTG GTGGACATGG ACGGCGTGCT AGCTGACTTC 
GAGTCCGGCC TTCTGCAGGG TTTCCGCCGT CGCTTCCCCG GGGAACCGCA TGTGCCGCTG 
GAACAGCGCC GGGGCTTCCT CGCCAGCGAG CAGTACGGAG CCCTGCGGCC GGACCTGGCG 
GAAAAAGTGG CCAGTGTCTA TGAAGCTCCG GGCTTTTTCC TAAACTTGGA GCCCATCCCT 
GGAGCCTTGG ACGCTTTGCG AGAGATGAAC GCCATGCAAG ACACCGATGT CTTCATCTGC 
ACCACCCCAC TGCGGAAGTA CGACCACTGT GTGGGCGAGA AGTACCGTTG GGTGGAGCAG 
AACCTGGGGC CACAGTTTGT GGAGCGGATT ATCCTGACTA GGGACAAGAC AGTGGTCATG 
GGAGACCTGC TCATTGATGA CAAAGACAAC ATTCAAGGCC TAGAGGAGAC CCCAAGCTGG 
GAGCACATCT TGTTCACCTG CTGCCACAAT CAGCACCTGG CCCTGCCCCC CACTAGGAGA 
CGGCTGCTTT CCTGGAGTGA TGACTGGAGG GGCATCATAG AGAGCAAGCG AGCCAGCCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258118.1
CDS	19..621
Translation	MAVKRPVRVLVDMDGVLADFESGLLQGFRRRFPGEPHVPLEQRRGFLASEQYGALRPDLAEKVASVYEAPGFFLNLEPIPGALDALREMNAMQDTDVFICTTPLRKYDHCVGEKYRWVEQNLGPQFVERIILTRDKTVVMGDLLIDDKDNIQGLEETPSWEHILFTCCHNQHLALPPTRRRLLSWSDDWRGIIESKRASL

Target ORF information:

RefSeq Version	NM_001271189.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 5', 3'-nucleotidase, cytosolic (Nt5c), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271189.1

ORF Insert Sequence:

ATGGCGGTGA AGCGGCCGGT GCGCGTGCTG GTGGACATGG ACGGCGTGCT AGCTGACTTC 
GAGTCCGGCC TTCTGCAGGG TTTCCGCCGT CGCTTCCCCG GGGAACCGCA TGTGCCGCTG 
GAACAGCGCC GGGGCTTCCT CGCCAGCGAG CAGTACGGAG CCCTGCGGCC GGACCTGGCG 
GAAAAAGTGG CCAGTGTCTA TGAAGCTCCG GGCTTTTTCC TAAACTTGGA GCCCATCCCT 
GGAGCCTTGG ACGCTTTGCG AGAGATGAAC GCCATGCAAG ACACCGATGT CTTCATCTGC 
ACCACCCCAC TGCGGAAGTA CGACCACTGT GTGGGCGAGA AGTACCGTTG GGTGGAGCAG 
AACCTGGGGC CACAGTTTGT GGAGCGGATT ATCCTGACTA GGGACAAGAC AGTGGTCATG 
GGAGACCTGC TCATTGATGA CAAAGACAAC ATTCAAGGCC TAGAGGAGAC CCCAAGCTGG 
GAGCACATCT TGTTCACCTG CTGCCACAAT CAGCACCTGG CCCTGCCCCC CACTAGGAGA 
CGGCTGCTTT CCTGGAGTGA TGACTGGAGG GGCATCATAG AGAGCAAGCG AGCCAGCCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

Mouse cytosolic and mitochondrial deoxyribonucleotidases: cDNA cloning of the mitochondrial enzyme, gene structures, chromosomal mapping and comparison with the human orthologs.
Gene294(1-2)109-17(2002 Jul)
Rampazzo C,Kost-Alimova M,Ruzzenente B,Dumanski JP,Bianchi V

Mammalian 5'(3')-deoxyribonucleotidase, cDNA cloning, and overexpression of the enzyme in Escherichia coli and mammalian cells.
The Journal of biological chemistry275(8)5409-15(2000 Feb)
Rampazzo C,Johansson M,Gallinaro L,Ferraro P,Hellman U,Karlsson A,Reichard P,Bianchi V

Chromosomal assignments of 23 biochemical loci of the rat by using rat x mouse somatic cell hybrids.
Cytogenetics and cell genetics57(2-3)142-8(1991)
Yasue M,Serikawa T,Yamada J

Cytoplasmic 5'(3')-nucleotidase from human placenta.
The Journal of biological chemistry265(12)6589-95(1990 Apr)
H?glund L,Reichard P