TRHDE cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRHDE
Entrez Gene ID	29953
Full Name	thyrotropin releasing hormone degrading enzyme
Synonyms	PAP-II,PGPEP2,TRH-DE
General protein information	Preferred Names thyrotropin releasing hormone degrading enzyme Names thyrotropin-releasing hormone-degrading ectoenzyme TRH-degrading ectoenzyme TRH-specific aminopeptidase pyroglutamyl aminopeptidase II pyroglutamyl-peptidase II thyroliberinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q21.1
Summary	This gene encodes a member of the peptidase M1 family. The encoded protein is an extracellular peptidase that specifically cleaves and inactivates the neuropeptide thyrotropin-releasing hormone.[provided by RefSeq, Dec 2008].
Disorder MIM:	606950

mRNA and Protein(s)

mRNA	Protein	Name
XM_005268819.4	XP_005268876.1	thyrotropin-releasing hormone-degrading ectoenzyme isoform X2
XM_011538248.2	XP_011536550.1	thyrotropin-releasing hormone-degrading ectoenzyme isoform X4
NM_013381.2	NP_037513.1	thyrotropin-releasing hormone-degrading ectoenzyme
XM_017019243.2	XP_016874732.2	thyrotropin-releasing hormone-degrading ectoenzyme isoform X1
XM_017019243.1	XP_016874732.1	thyrotropin-releasing hormone-degrading ectoenzyme isoform X1
XM_005268819.5	XP_005268876.2	thyrotropin-releasing hormone-degrading ectoenzyme isoform X2
XM_017019244.1	XP_016874733.1	thyrotropin-releasing hormone-degrading ectoenzyme isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRHDE	NP_037513.1
Pan troglodytes (chimpanzee)	TRHDE	XP_522471.4
Canis lupus familiaris (dog)	TRHDE	XP_005625720.1
Xenopus tropicalis (tropical clawed frog)	trhde	XP_002939988.2
	TRHDE	XP_001109391.2
Bos taurus (cattle)	TRHDE	XP_002687216.3
Rattus norvegicus (Norway rat)	Trhde	NP_001102461.1
Mus musculus (house mouse)	Trhde	NP_666353.1
Gallus gallus (chicken)	TRHDE	XP_425442.3
Danio rerio (zebrafish)	LOC562409	XP_690889.2
Danio rerio (zebrafish)	si:ch211-149p5.1	XP_001345457.3

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide association study of a heart failure related metabolomic profile among African Americans in the Atherosclerosis Risk in Communities (ARIC) study.
Yu B, Zheng Y, Alexander D, Manolio TA, Alonso A, Nettleton JA, Boerwinkle E
Genetic epidemiology37(8)840-5(2013 Dec)

Genome-wide association study of rheumatoid arthritis in Koreans: population-specific loci as well as overlap with European susceptibility loci.
Freudenberg J, Lee HS, Han BG, Shin HD, Kang YM, Sung YK, Shim SC, Choi CB, Lee AT, Gregersen PK, Bae SC
Arthritis and rheumatism63(4)884-93(2011 Apr)

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Bailey SD, Xie C, Do R, Montpetit A, Diaz R, Mohan V, Keavney B, Yusuf S, Gerstein HC, Engert JC, Anand S,
Diabetes care33(10)2250-3(2010 Oct)

Chemerin, a novel adipokine in the regulation of angiogenesis.
Bozaoglu K, Curran JE, Stocker CJ, Zaibi MS, Segal D, Konstantopoulos N, Morrison S, Carless M, Dyer TD, Cole SA, Goring HH, Moses EK, Walder K, Cawthorne MA, Blangero J, Jowett JB
The Journal of clinical endocrinology and metabolism95(5)2476-85(2010 May)

Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.
Talmud PJ, Drenos F, Shah S, Shah T, Palmen J, Verzilli C, Gaunt TR, Pallas J, Lovering R, Li K, Casas JP, Sofat R, Kumari M, Rodriguez S, Johnson T, Newhouse SJ, Dominiczak A, Samani NJ, Caulfield M, Sever P, Stanton A, Shields DC, Padmanabhan S, Melander O, Hastie C, Delles C, Ebrahim S, Marmot MG, Smith GD, Lawlor DA, Munroe PB, Day IN, Kivimaki M, Whittaker J, Humphries SE, Hingorani AD, , ,
American journal of human genetics85(5)628-42(2009 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

The following TRHDE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRHDE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26282	NM_013381.2 Latest version!	Homo sapiens thyrotropin releasing hormone degrading enzyme (TRHDE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu67425	XM_011538248.2 Latest version!	Homo sapiens thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OHu83743	XM_017019244.1 Latest version!	Homo sapiens thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$391.30 $559.00
OHu46507	XM_005268819.4 Latest version!	Homo sapiens thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu103716	XM_017019243.2 Latest version!	Homo sapiens thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu103717	XM_005268819.5 Latest version!	Homo sapiens thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu83742	XM_017019243.1 Latest version!	Homo sapiens thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRHDE ( NM_013381.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_037513.1 Homo sapiens thyrotropin releasing hormone degrading enzyme (TRHDE), mRNA.

Price & Availability↑

CloneID	OHu26282
Clone ID Related Accession (Same CDS sequence)	NM_013381.2
Accession Version	NM_013381.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	thyrotropin-releasing hormone-degrading ectoenzyme
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC142706.1 and AC133480.2. This sequence is a reference standard in the RefSeqGene project. On Dec 24, 2008 this sequence version replaced NM_013381.1. ##Evidence-Data-START## Transcript exon combination :: AF126372.1, SRR1660809.247852.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGGAAG ACGACGCCGC GCTTCGGGCT GGCAGCAGGG GGCTCTCCGA CCCGTGGGCA 
GACTCAGTGG GAGTGCGACC CCGCACCACG GAGCGCCACA TCGCCGTACA CAAGCGGCTT 
GTGCTGGCCT TCGCTGTGTC CCTCGTGGCA TTGCTCGCGG TCACAATGCT CGCTGTGCTG 
CTCAGCCTGC GCTTCGACGA GTGCGGGGCG AGTGCCACGC CAGGCGCCGA CGGTGGCCCC 
TCAGGCTTTC CGGAGCGCGG CGGCAACGGG AGCCTCCCTG GATCGGCCCG GCGCAACCAC 
CACGCAGGCG GGGACTCCTG GCAGCCCGAG GCGGGTGGGG TGGCCAGTCC GGGGACCACG 
TCGGCCCAGC CGCCGTCGGA GGAGGAGCGG GAGCCGTGGG AGCCGTGGAC GCAGCTGCGC 
CTGTCGGGCC ACCTGAAGCC GCTGCACTAC AATCTGATGC TCACCGCCTT CATGGAGAAC 
TTCACCTTCT CCGGGGAGGT CAACGTGGAG ATCGCGTGCC GGAACGCCAC CCGCTACGTA 
GTGCTGCACG CTTCCCGAGT GGCGGTGGAG AAAGTGCAGC TGGCCGAGGA CCGGGCGTTC 
GGGGCTGTCC CTGTAGCCGG TTTTTTCCTC TACCCGCAAA CCCAGGTCTT AGTGGTGGTG 
CTGAATAGGA CACTGGACGC GCAGAGGAAT TACAATCTGA AGATTATCTA CAACGCGCTC 
ATCGAGAATG AGCTCCTGGG CTTCTTCCGC AGCTCCTATG TGCTCCACGG GGAGAGAAGA 
TTCCTTGGTG TTACTCAGTT TTCGCCTACA CATGCCAGAA AGGCATTTCC TTGTTTTGAT 
GAGCCAATCT ACAAGGCTAC TTTCAAAATC AGCATCAAGC ATCAAGCAAC CTATTTATCT 
TTATCTAATA TGCCAGTGGA AACTTCCGTG TTTGAGGAAG ATGGATGGGT TACGGATCAC 
TTTTCACAGA CCCCTCTCAT GTCCACATAT TATTTAGCCT GGGCAATTTG CAACTTCACA 
TACAGAGAAA CTACCACCAA GAGTGGGGTT GTAGTACGAT TATATGCAAG ACCTGATGCT 
ATCAGAAGAG GATCCGGGGA CTATGCTCTC CATATAACAA AGAGATTAAT AGAATTTTAT 
GAAGACTACT TTAAAGTGCC CTATTCCTTG CCAAAACTAG ATCTTTTAGC TGTGCCTAAG 
CATCCGTATG CTGCTATGGA GAACTGGGGA CTAAGTATTT TTGTGGAACA AAGAATACTG 
CTGGATCCCA GTGTTTCATC TATTTCTTAT TTGCTGGATG TCACCATGGT CATTGTTCAT 
GAGATATGTC ACCAGTGGTT TGGTGACCTT GTGACGCCTG TGTGGTGGGA AGACGTGTGG 
CTGAAGGAAG GGTTTGCTCA CTACTTTGAA TTTGTTGGTA CAGACTACCT CTATCCTGGC 
TGGAACATGG AAAAGCAGAG GTTTCTGACC GATGTTCTGC ATGAAGTGAT GCTGCTGGAC 
GGTTTGGCCA GTTCCCATCC AGTATCACAG GAAGTGCTGC AGGCAACAGA TATTGACAGG 
GTGTTTGACT GGATCGCATA TAAAAAGGGT GCTGCTTTAA TAAGAATGCT GGCTAATTTT 
ATGGGCCATT CAGTTTTCCA GAGGGGTTTG CAAGATTATT TAACCATTCA TAAGTATGGT 
AATGCAGCCA GAAATGATCT CTGGAATACA TTATCGGAGG CTTTAAAAAG AAATGGGAAA 
TATGTAAATA TACAAGAAGT AATGGATCAG TGGACACTCC AGATGGGTTA TCCTGTTATC 
ACCATCTTGG GAAACACAAC AGCAGAAAAT AGAATAATAA TTACCCAACA GCATTTTATC 
TATGATATCA GTGCTAAAAC TAAAGCACTT AAACTTCAGA ATAACAGTTA CCTGTGGCAG 
ATTCCATTAA CTATTGTGGT AGGAAATAGA AGCCATGTGT CTTCAGAAGC AATTATTTGG 
GTGTCTAACA AATCAGAGCA CCACAGAATA ACTTATTTGG ACAAAGGAAG CTGGCTGCTG 
GGGAACATCA ATCAAACTGG CTATTTTAGA GTCAACTATG ACCTAAGGAA CTGGAGATTA 
TTAATTGATC AATTAATCCG GAATCATGAG GTTCTTTCTG TCAGTAACCG AGCGGGCTTG 
ATCGATGATG CCTTCAGCCT AGCCAGGGCT GGCTATTTGC CTCAGAATAT TCCTCTGGAG 
ATTATCAGAT ACCTGTCTGA GGAGAAGGAT TTTCTTCCTT GGCATGCTGC CAGCCGAGCT 
CTTTATCCTC TAGATAAATT ACTGGACCGC ATGGAAAACT ACAACATTTT CAATGAATAT 
ATTTTAAAGC AAGTTGCAAC AACATATATC AAGCTTGGGT GGCCGAAAAA TAATTTTAAT 
GGATCTCTTG TTCAAGCATC CTACCAACAT GAAGAACTAC GTAGAGAAGT TATAATGCTG 
GCCTGCAGTT TTGGCAACAA GCACTGTCAC CAACAGGCAT CAACACTTAT TTCAGATTGG 
ATTTCCAGCA ACAGGAACAG AATACCACTA AATGTTAGAG ACATCGTATA CTGTACAGGA 
GTGTCACTAC TGGATGAGGA TGTCTGGGAA TTCATATGGA TGAAATTCCA TTCCACCACA 
GCAGTTTCTG AGAAGAAAAT ATTATTGGAA GCCTTAACTT GCAGTGATGA CAGGAATTTA 
TTAAACAGGC TTCTAAATCT GTCACTGAAT TCTGAGGTGG TGCTGGATCA AGATGCAATT 
GATGTCATAA TCCATGTAGC TCGAAATCCA CATGGTCGAG ACCTTGCCTG GAAGTTTTTC 
AGGGATAAAT GGAAGATATT AAATACCAGG TATGGAGAAG CATTGTTTAT GAATTCCAAA 
CTCATCAGTG GTGTCACAGA ATTTCTTAAT ACTGAAGGTG AACTCAAAGA GCTCAAGAAC 
TTCATGAAAA ACTATGATGG GGTAGCTGCT GCTTCTTTCT CACGAGCTGT GGAAACTGTC 
GAAGCCAATG TGCGCTGGAA AATGCTTTAC CAAGACGAGC TTTTCCAATG GTTAGGAAAA 
GCTCTAAGAC ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037513.1
CDS	31..3105
Translation	MGEDDAALRAGSRGLSDPWADSVGVRPRTTERHIAVHKRLVLAFAVSLVALLAVTMLAVLLSLRFDECGASATPGADGGPSGFPERGGNGSLPGSARRNHHAGGDSWQPEAGGVASPGTTSAQPPSEEEREPWEPWTQLRLSGHLKPLHYNLMLTAFMENFTFSGEVNVEIACRNATRYVVLHASRVAVEKVQLAEDRAFGAVPVAGFFLYPQTQVLVVVLNRTLDAQRNYNLKIIYNALIENELLGFFRSSYVLHGERRFLGVTQFSPTHARKAFPCFDEPIYKATFKISIKHQATYLSLSNMPVETSVFEEDGWVTDHFSQTPLMSTYYLAWAICNFTYRETTTKSGVVVRLYARPDAIRRGSGDYALHITKRLIEFYEDYFKVPYSLPKLDLLAVPKHPYAAMENWGLSIFVEQRILLDPSVSSISYLLDVTMVIVHEICHQWFGDLVTPVWWEDVWLKEGFAHYFEFVGTDYLYPGWNMEKQRFLTDVLHEVMLLDGLASSHPVSQEVLQATDIDRVFDWIAYKKGAALIRMLANFMGHSVFQRGLQDYLTIHKYGNAARNDLWNTLSEALKRNGKYVNIQEVMDQWTLQMGYPVITILGNTTAENRIIITQQHFIYDISAKTKALKLQNNSYLWQIPLTIVVGNRSHVSSEAIIWVSNKSEHHRITYLDKGSWLLGNINQTGYFRVNYDLRNWRLLIDQLIRNHEVLSVSNRAGLIDDAFSLARAGYLPQNIPLEIIRYLSEEKDFLPWHAASRALYPLDKLLDRMENYNIFNEYILKQVATTYIKLGWPKNNFNGSLVQASYQHEELRREVIMLACSFGNKHCHQQASTLISDWISSNRNRIPLNVRDIVYCTGVSLLDEDVWEFIWMKFHSTTAVSEKKILLEALTCSDDRNLLNRLLNLSLNSEVVLDQDAIDVIIHVARNPHGRDLAWKFFRDKWKILNTRYGEALFMNSKLISGVTEFLNTEGELKELKNFMKNYDGVAAASFSRAVETVEANVRWKMLYQDELFQWLGKALRH

Target ORF information:

RefSeq Version	NM_013381.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thyrotropin releasing hormone degrading enzyme (TRHDE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013381.2

ORF Insert Sequence:

ATGGGGGAAG ACGACGCCGC GCTTCGGGCT GGCAGCAGGG GGCTCTCCGA CCCGTGGGCA 
GACTCAGTGG GAGTGCGACC CCGCACCACG GAGCGCCACA TCGCCGTACA CAAGCGGCTT 
GTGCTGGCCT TCGCTGTGTC CCTCGTGGCA TTGCTCGCGG TCACAATGCT CGCTGTGCTG 
CTCAGCCTGC GCTTCGACGA GTGCGGGGCG AGTGCCACGC CAGGCGCCGA CGGTGGCCCC 
TCAGGCTTTC CGGAGCGCGG CGGCAACGGG AGCCTCCCTG GATCGGCCCG GCGCAACCAC 
CACGCAGGCG GGGACTCCTG GCAGCCCGAG GCGGGTGGGG TGGCCAGTCC GGGGACCACG 
TCGGCCCAGC CGCCGTCGGA GGAGGAGCGG GAGCCGTGGG AGCCGTGGAC GCAGCTGCGC 
CTGTCGGGCC ACCTGAAGCC GCTGCACTAC AATCTGATGC TCACCGCCTT CATGGAGAAC 
TTCACCTTCT CCGGGGAGGT CAACGTGGAG ATCGCGTGCC GGAACGCCAC CCGCTACGTA 
GTGCTGCACG CTTCCCGAGT GGCGGTGGAG AAAGTGCAGC TGGCCGAGGA CCGGGCGTTC 
GGGGCTGTCC CTGTAGCCGG TTTTTTCCTC TACCCGCAAA CCCAGGTCTT AGTGGTGGTG 
CTGAATAGGA CACTGGACGC GCAGAGGAAT TACAATCTGA AGATTATCTA CAACGCGCTC 
ATCGAGAATG AGCTCCTGGG CTTCTTCCGC AGCTCCTATG TGCTCCACGG GGAGAGAAGA 
TTCCTTGGTG TTACTCAGTT TTCGCCTACA CATGCCAGAA AGGCATTTCC TTGTTTTGAT 
GAGCCAATCT ACAAGGCTAC TTTCAAAATC AGCATCAAGC ATCAAGCAAC CTATTTATCT 
TTATCTAATA TGCCAGTGGA AACTTCCGTG TTTGAGGAAG ATGGATGGGT TACGGATCAC 
TTTTCACAGA CCCCTCTCAT GTCCACATAT TATTTAGCCT GGGCAATTTG CAACTTCACA 
TACAGAGAAA CTACCACCAA GAGTGGGGTT GTAGTACGAT TATATGCAAG ACCTGATGCT 
ATCAGAAGAG GATCCGGGGA CTATGCTCTC CATATAACAA AGAGATTAAT AGAATTTTAT 
GAAGACTACT TTAAAGTGCC CTATTCCTTG CCAAAACTAG ATCTTTTAGC TGTGCCTAAG 
CATCCGTATG CTGCTATGGA GAACTGGGGA CTAAGTATTT TTGTGGAACA AAGAATACTG 
CTGGATCCCA GTGTTTCATC TATTTCTTAT TTGCTGGATG TCACCATGGT CATTGTTCAT 
GAGATATGTC ACCAGTGGTT TGGTGACCTT GTGACGCCTG TGTGGTGGGA AGACGTGTGG 
CTGAAGGAAG GGTTTGCTCA CTACTTTGAA TTTGTTGGTA CAGACTACCT CTATCCTGGC 
TGGAACATGG AAAAGCAGAG GTTTCTGACC GATGTTCTGC ATGAAGTGAT GCTGCTGGAC 
GGTTTGGCCA GTTCCCATCC AGTATCACAG GAAGTGCTGC AGGCAACAGA TATTGACAGG 
GTGTTTGACT GGATCGCATA TAAAAAGGGT GCTGCTTTAA TAAGAATGCT GGCTAATTTT 
ATGGGCCATT CAGTTTTCCA GAGGGGTTTG CAAGATTATT TAACCATTCA TAAGTATGGT 
AATGCAGCCA GAAATGATCT CTGGAATACA TTATCGGAGG CTTTAAAAAG AAATGGGAAA 
TATGTAAATA TACAAGAAGT AATGGATCAG TGGACACTCC AGATGGGTTA TCCTGTTATC 
ACCATCTTGG GAAACACAAC AGCAGAAAAT AGAATAATAA TTACCCAACA GCATTTTATC 
TATGATATCA GTGCTAAAAC TAAAGCACTT AAACTTCAGA ATAACAGTTA CCTGTGGCAG 
ATTCCATTAA CTATTGTGGT AGGAAATAGA AGCCATGTGT CTTCAGAAGC AATTATTTGG 
GTGTCTAACA AATCAGAGCA CCACAGAATA ACTTATTTGG ACAAAGGAAG CTGGCTGCTG 
GGGAACATCA ATCAAACTGG CTATTTTAGA GTCAACTATG ACCTAAGGAA CTGGAGATTA 
TTAATTGATC AATTAATCCG GAATCATGAG GTTCTTTCTG TCAGTAACCG AGCGGGCTTG 
ATCGATGATG CCTTCAGCCT AGCCAGGGCT GGCTATTTGC CTCAGAATAT TCCTCTGGAG 
ATTATCAGAT ACCTGTCTGA GGAGAAGGAT TTTCTTCCTT GGCATGCTGC CAGCCGAGCT 
CTTTATCCTC TAGATAAATT ACTGGACCGC ATGGAAAACT ACAACATTTT CAATGAATAT 
ATTTTAAAGC AAGTTGCAAC AACATATATC AAGCTTGGGT GGCCGAAAAA TAATTTTAAT 
GGATCTCTTG TTCAAGCATC CTACCAACAT GAAGAACTAC GTAGAGAAGT TATAATGCTG 
GCCTGCAGTT TTGGCAACAA GCACTGTCAC CAACAGGCAT CAACACTTAT TTCAGATTGG 
ATTTCCAGCA ACAGGAACAG AATACCACTA AATGTTAGAG ACATCGTATA CTGTACAGGA 
GTGTCACTAC TGGATGAGGA TGTCTGGGAA TTCATATGGA TGAAATTCCA TTCCACCACA 
GCAGTTTCTG AGAAGAAAAT ATTATTGGAA GCCTTAACTT GCAGTGATGA CAGGAATTTA 
TTAAACAGGC TTCTAAATCT GTCACTGAAT TCTGAGGTGG TGCTGGATCA AGATGCAATT 
GATGTCATAA TCCATGTAGC TCGAAATCCA CATGGTCGAG ACCTTGCCTG GAAGTTTTTC 
AGGGATAAAT GGAAGATATT AAATACCAGG TATGGAGAAG CATTGTTTAT GAATTCCAAA 
CTCATCAGTG GTGTCACAGA ATTTCTTAAT ACTGAAGGTG AACTCAAAGA GCTCAAGAAC 
TTCATGAAAA ACTATGATGG GGTAGCTGCT GCTTCTTTCT CACGAGCTGT GGAAACTGTC 
GAAGCCAATG TGCGCTGGAA AATGCTTTAC CAAGACGAGC TTTTCCAATG GTTAGGAAAA 
GCTCTAAGAC ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRHDE ( XM_011538248.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536550.1 Homo sapiens thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu67425
Clone ID Related Accession (Same CDS sequence)	XM_011538248.2
Accession Version	XM_011538248.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	thyrotropin-releasing hormone-degrading ectoenzyme isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538248.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACT GGGGACTAAG TATTTTTGTG GAACAAAGAA TACTGCTGGA TCCCAGTGTT 
TCATCTATTT CTTATTTGCT GGATGTCACC ATGGTCATTG TTCATGAGAT ATGTCACCAG 
TGGTTTGGTG ACCTTGTGAC GCCTGTGTGG TGGGAAGACG TGTGGCTGAA GGAAGGGTTT 
GCTCACTACT TTGAATTTGT TGGTACAGAC TACCTCTATC CTGGCTGGAA CATGGAAAAG 
CAGAGGTTTC TGACCGATGT TCTGCATGAA GTGATGCTGC TGGACGGTTT GGCCAGTTCC 
CATCCAGTAT CACAGGAAGT GCTGCAGGCA ACAGATATTG ACAGGGTGTT TGACTGGATC 
GCATATAAAA AGGGTGCTGC TTTAATAAGA ATGCTGGCTA ATTTTATGGG CCATTCAGTT 
TTCCAGAGGG GTTTGCAAGA TTATTTAACC ATTCATAAGT ATGGTAATGC AGCCAGAAAT 
GATCTCTGGA ATACATTATC GGAGGCTTTA AAAAGAAATG GGAAATATGT AAATATACAA 
GAAGTAATGG ATCAGTGGAC ACTCCAGATG GGTTATCCTG TTATCACCAT CTTGGGAAAC 
ACAACAGCAG AAAATAGAAT AATAATTACC CAACAGCATT TTATCTATGA TATCAGTGCT 
AAAACTAAAG CACTTAAACT TCAGAATAAC AGTTACCTGT GGCAGATTCC ATTAACTATT 
GTGGTAGGAA ATAGAAGCCA TGTGTCTTCA GAAGCAATTA TTTGGGTGTC TAACAAATCA 
GAGCACCACA GAATAACTTA TTTGGACAAA GGAAGCTGGC TGCTGGGGAA CATCAATCAA 
ACTGGCTATT TTAGAGTCAA CTATGACCTA AGGAACTGGA GATTATTAAT TGATCAATTA 
ATCCGGAATC ATGAGGTTCT TTCTGTCAGT AACCGAGCGG GCTTGATCGA TGATGCCTTC 
AGCCTAGCCA GGGCTGGCTA TTTGCCTCAG AATATTCCTC TGGAGATTAT CAGATACCTG 
TCTGAGGAGA AGGATTTTCT TCCTTGGCAT GCTGCCAGCC GAGCTCTTTA TCCTCTAGAT 
AAATTACTGG ACCGCATGGA AAACTACAAC ATTTTCAATG AATATATTTT AAAGCAAGTT 
GCAACAACAT ATATCAAGCT TGGGTGGCCG AAAAATAATT TTAATGGATC TCTTGTTCAA 
GCATCCTACC AACATGAAGA ACTACGTAGA GAAGTTATAA TGCTGGCCTG CAGTTTTGGC 
AACAAGCACT GTCACCAACA GGCATCAACA CTTATTTCAG ATTGGATTTC CAGCAACAGG 
AACAGAATAC CACTAAATGT TAGAGACATC GTATACTGTA CAGGAGTGTC ACTACTGGAT 
GAGGATGTCT GGGAATTCAT ATGGATGAAA TTCCATTCCA CCACAGCAGT TTCTGAGAAG 
AAAATATTAT TGGAAGCCTT AACTTGCAGT GATGACAGGA ATTTATTAAA CAGGCTTCTA 
AATCTGTCAC TGAATTCTGA GGTGGTGCTG GATCAAGATG CAATTGATGT CATAATCCAT 
GTAGCTCGAA ATCCACATGG TCGAGACCTT GCCTGGAAGT TTTTCAGGGA TAAATGGAAG 
ATATTAAATA CCAGGTATGG AGAAGCATTG TTTATGAATT CCAAACTCAT CAGTGGTGTC 
ACAGAATTTC TTAATACTGA AGGTGAACTC AAAGAGCTCA AGAACTTCAT GAAAAACTAT 
GATGGGGTAG CTGCTGCTTC TTTCTCACGA GCTGTGGAAA CTGTCGAAGC CAATGTGCGC 
TGGAAAATGC TTTACCAAGA CGAGCTTTTC CAATGGTTAG GAAAAGCTCT AAGACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536550.1
CDS	1424..3283
Translation	MENWGLSIFVEQRILLDPSVSSISYLLDVTMVIVHEICHQWFGDLVTPVWWEDVWLKEGFAHYFEFVGTDYLYPGWNMEKQRFLTDVLHEVMLLDGLASSHPVSQEVLQATDIDRVFDWIAYKKGAALIRMLANFMGHSVFQRGLQDYLTIHKYGNAARNDLWNTLSEALKRNGKYVNIQEVMDQWTLQMGYPVITILGNTTAENRIIITQQHFIYDISAKTKALKLQNNSYLWQIPLTIVVGNRSHVSSEAIIWVSNKSEHHRITYLDKGSWLLGNINQTGYFRVNYDLRNWRLLIDQLIRNHEVLSVSNRAGLIDDAFSLARAGYLPQNIPLEIIRYLSEEKDFLPWHAASRALYPLDKLLDRMENYNIFNEYILKQVATTYIKLGWPKNNFNGSLVQASYQHEELRREVIMLACSFGNKHCHQQASTLISDWISSNRNRIPLNVRDIVYCTGVSLLDEDVWEFIWMKFHSTTAVSEKKILLEALTCSDDRNLLNRLLNLSLNSEVVLDQDAIDVIIHVARNPHGRDLAWKFFRDKWKILNTRYGEALFMNSKLISGVTEFLNTEGELKELKNFMKNYDGVAAASFSRAVETVEANVRWKMLYQDELFQWLGKALRH

Target ORF information:

RefSeq Version	XM_011538248.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538248.2

ORF Insert Sequence:

ATGGAGAACT GGGGACTAAG TATTTTTGTG GAACAAAGAA TACTGCTGGA TCCCAGTGTT 
TCATCTATTT CTTATTTGCT GGATGTCACC ATGGTCATTG TTCATGAGAT ATGTCACCAG 
TGGTTTGGTG ACCTTGTGAC GCCTGTGTGG TGGGAAGACG TGTGGCTGAA GGAAGGGTTT 
GCTCACTACT TTGAATTTGT TGGTACAGAC TACCTCTATC CTGGCTGGAA CATGGAAAAG 
CAGAGGTTTC TGACCGATGT TCTGCATGAA GTGATGCTGC TGGACGGTTT GGCCAGTTCC 
CATCCAGTAT CACAGGAAGT GCTGCAGGCA ACAGATATTG ACAGGGTGTT TGACTGGATC 
GCATATAAAA AGGGTGCTGC TTTAATAAGA ATGCTGGCTA ATTTTATGGG CCATTCAGTT 
TTCCAGAGGG GTTTGCAAGA TTATTTAACC ATTCATAAGT ATGGTAATGC AGCCAGAAAT 
GATCTCTGGA ATACATTATC GGAGGCTTTA AAAAGAAATG GGAAATATGT AAATATACAA 
GAAGTAATGG ATCAGTGGAC ACTCCAGATG GGTTATCCTG TTATCACCAT CTTGGGAAAC 
ACAACAGCAG AAAATAGAAT AATAATTACC CAACAGCATT TTATCTATGA TATCAGTGCT 
AAAACTAAAG CACTTAAACT TCAGAATAAC AGTTACCTGT GGCAGATTCC ATTAACTATT 
GTGGTAGGAA ATAGAAGCCA TGTGTCTTCA GAAGCAATTA TTTGGGTGTC TAACAAATCA 
GAGCACCACA GAATAACTTA TTTGGACAAA GGAAGCTGGC TGCTGGGGAA CATCAATCAA 
ACTGGCTATT TTAGAGTCAA CTATGACCTA AGGAACTGGA GATTATTAAT TGATCAATTA 
ATCCGGAATC ATGAGGTTCT TTCTGTCAGT AACCGAGCGG GCTTGATCGA TGATGCCTTC 
AGCCTAGCCA GGGCTGGCTA TTTGCCTCAG AATATTCCTC TGGAGATTAT CAGATACCTG 
TCTGAGGAGA AGGATTTTCT TCCTTGGCAT GCTGCCAGCC GAGCTCTTTA TCCTCTAGAT 
AAATTACTGG ACCGCATGGA AAACTACAAC ATTTTCAATG AATATATTTT AAAGCAAGTT 
GCAACAACAT ATATCAAGCT TGGGTGGCCG AAAAATAATT TTAATGGATC TCTTGTTCAA 
GCATCCTACC AACATGAAGA ACTACGTAGA GAAGTTATAA TGCTGGCCTG CAGTTTTGGC 
AACAAGCACT GTCACCAACA GGCATCAACA CTTATTTCAG ATTGGATTTC CAGCAACAGG 
AACAGAATAC CACTAAATGT TAGAGACATC GTATACTGTA CAGGAGTGTC ACTACTGGAT 
GAGGATGTCT GGGAATTCAT ATGGATGAAA TTCCATTCCA CCACAGCAGT TTCTGAGAAG 
AAAATATTAT TGGAAGCCTT AACTTGCAGT GATGACAGGA ATTTATTAAA CAGGCTTCTA 
AATCTGTCAC TGAATTCTGA GGTGGTGCTG GATCAAGATG CAATTGATGT CATAATCCAT 
GTAGCTCGAA ATCCACATGG TCGAGACCTT GCCTGGAAGT TTTTCAGGGA TAAATGGAAG 
ATATTAAATA CCAGGTATGG AGAAGCATTG TTTATGAATT CCAAACTCAT CAGTGGTGTC 
ACAGAATTTC TTAATACTGA AGGTGAACTC AAAGAGCTCA AGAACTTCAT GAAAAACTAT 
GATGGGGTAG CTGCTGCTTC TTTCTCACGA GCTGTGGAAA CTGTCGAAGC CAATGTGCGC 
TGGAAAATGC TTTACCAAGA CGAGCTTTTC CAATGGTTAG GAAAAGCTCT AAGACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRHDE ( XM_017019244.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874733.1 Homo sapiens thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu83743
Clone ID Related Accession (Same CDS sequence)	XM_017019244.1
Accession Version	XM_017019244.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	thyrotropin-releasing hormone-degrading ectoenzyme isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGTGG AAACTTCCGT GTTTGAGGAA GATGGATGGG TTACGGATCA CTTTTCACAG 
ACCCCTCTCA TGTCCACATA TTATTTAGCC TGGGCAATTT GCAACTTCAC ATACAGAGAA 
ACTACCACCA AGAGTGGGGT TGTAGTACGA TTATATGCAA GACCTGATGC TATCAGAAGA 
GGATCCGGGG ACTATGCTCT CCATATAACA AAGAGATTAA TAGAATTTTA TGAAGACTAC 
TTTAAAGTGC CCTATTCCTT GCCAAAACTA GATCTTTTAG CTGTGCCTAA GCATCCGTAT 
GCTGCTATGG AGAACTGGGG ACTAAGTATT TTTGTGGAAC AAAGAATACT GCTGGATCCC 
AGTGTTTCAT CTATTTCTTA TTTGCTGGAT GTCACCATGG TCATTGTTCA TGAGATATGT 
CACCAGTGGT TTGGTGACCT TGTGACGCCT GTGTGGTGGG AAGACGTGTG GCTGAAGGAA 
GGGTTTGCTC ACTACTTTGA ATTTGTTGGT ACAGACTACC TCTATCCTGG CTGGAACATG 
GAAAAGCAGA GGTTTCTGAC CGATGTTCTG CATGAAGTGA TGCTGCTGGA CGGTTTGGCC 
AGTTCCCATC CAGTATCACA GGAAGTGCTG CAGGCAACAG ATATTGACAG GGTGTTTGAC 
TGGATCGCAT ATAAAAAGGG TGCTGCTTTA ATAAGAATGC TGGCTAATTT TATGGGCCAT 
TCAGTTTTCC AGAGGGGTTT GCAAGATTAT TTAACCATTC ATAAGTATGG TAATGCAGCC 
AGAAATGATC TCTGGAATAC ATTATCGGAG GCTTTAAAAA GAAATGGGAA ATATGTAAAT 
ATACAAGAAG TAATGGATCA GTGGACACTC CAGATGGGTT ATCCTGTTAT CACCATCTTG 
GGAAACACAA CAGCAGAAAA TAGAATAATA ATTACCCAAC AGCATTTTAT CTATGATATC 
AGTGCTAAAA CTAAAGCACT TAAACTTCAG AATAACAGTT ACCTGTGGCA GATTCCATTA 
ACTATTGTGG TAGGAAATAG AAGCCATGTG TCTTCAGAAG CAATTATTTG GGTGTCTAAC 
AAATCAGAGC ACCACAGAAT AACTTATTTG GACAAAGGAA GCTGGCTGCT GGGGAACATC 
AATCAAACTG GCTATTTTAG AGTCAACTAT GACCTAAGGA ACTGGAGATT ATTAATTGAT 
CAATTAATCC GGAATCATGA GGTTCTTTCT GTCAGTAACC GAGCGGGCTT GATCGATGAT 
GCCTTCAGCC TAGCCAGGGC TGGCTATTTG CCTCAGAATA TTCCTCTGGA GATTATCAGA 
TACCTGTCTG AGGAGAAGGA TTTTCTTCCT TGGCATGCTG CCAGCCGAGC TCTTTATCCT 
CTAGATAAAT TACTGGACCG CATGGAAAAC TACAACATTT TCAATGAATA TATTTTAAAG 
CAAGTTGCAA CAACATATAT CAAGCTTGGG TGGCCGAAAA ATAATTTTAA TGGATCTCTT 
GTTCAAGCAT CCTACCAACA TGAAGAACTA CGTAGAGAAG TTATAATGCT GGCCTGCAGT 
TTTGGCAACA AGCACTGTCA CCAACAGGCA TCAACACTTA TTTCAGATTG GATTTCCAGC 
AACAGGAACA GAATACCACT AAATGTTAGA GACATCGTAT ACTGTACAGG AGTGTCACTA 
CTGGATGAGG ATGTCTGGGA ATTCATATGG ATGAAATTCC ATTCCACCAC AGCAGTTTCT 
GAGAAGAAAA TATTATTGGA AGCCTTAACT TGCAGTGATG ACAGGAATTT ATTAAACAGG 
CTTCTAAATC TGTCACTGAA TTCTGAGGTG GTGCTGGATC AAGATGCAAT TGATGTCATA 
ATCCATGTAG CTCGAAATCC ACATGGTCGA GACCTTGCCT GGAAGTTTTT CAGGGATAAA 
TGGAAGATAT TAAATACCAG GTATGGAGAA GCATTGTTTA TGAATTCCAA ACTCATCAGT 
GGTGTCACAG AATTTCTTAA TACTGAAGGT GAACTCAAAG AGCTCAAGAA CTTCATGAAA 
AACTATGATG GGGTAGCTGC TGCTTCTTTC TCACGAGCTG TGGAAACTGT CGAAGCCAAT 
GTGCGCTGGA AAATGCTTTA CCAAGACGAG CTTTTCCAAT GGTTAGGAAA AGCTCTAAGA 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874733.1
CDS	388..2553
Translation	MPVETSVFEEDGWVTDHFSQTPLMSTYYLAWAICNFTYRETTTKSGVVVRLYARPDAIRRGSGDYALHITKRLIEFYEDYFKVPYSLPKLDLLAVPKHPYAAMENWGLSIFVEQRILLDPSVSSISYLLDVTMVIVHEICHQWFGDLVTPVWWEDVWLKEGFAHYFEFVGTDYLYPGWNMEKQRFLTDVLHEVMLLDGLASSHPVSQEVLQATDIDRVFDWIAYKKGAALIRMLANFMGHSVFQRGLQDYLTIHKYGNAARNDLWNTLSEALKRNGKYVNIQEVMDQWTLQMGYPVITILGNTTAENRIIITQQHFIYDISAKTKALKLQNNSYLWQIPLTIVVGNRSHVSSEAIIWVSNKSEHHRITYLDKGSWLLGNINQTGYFRVNYDLRNWRLLIDQLIRNHEVLSVSNRAGLIDDAFSLARAGYLPQNIPLEIIRYLSEEKDFLPWHAASRALYPLDKLLDRMENYNIFNEYILKQVATTYIKLGWPKNNFNGSLVQASYQHEELRREVIMLACSFGNKHCHQQASTLISDWISSNRNRIPLNVRDIVYCTGVSLLDEDVWEFIWMKFHSTTAVSEKKILLEALTCSDDRNLLNRLLNLSLNSEVVLDQDAIDVIIHVARNPHGRDLAWKFFRDKWKILNTRYGEALFMNSKLISGVTEFLNTEGELKELKNFMKNYDGVAAASFSRAVETVEANVRWKMLYQDELFQWLGKALRH

Target ORF information:

RefSeq Version	XM_017019244.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019244.1

ORF Insert Sequence:

ATGCCAGTGG AAACTTCCGT GTTTGAGGAA GATGGATGGG TTACGGATCA CTTTTCACAG 
ACCCCTCTCA TGTCCACATA TTATTTAGCC TGGGCAATTT GCAACTTCAC ATACAGAGAA 
ACTACCACCA AGAGTGGGGT TGTAGTACGA TTATATGCAA GACCTGATGC TATCAGAAGA 
GGATCCGGGG ACTATGCTCT CCATATAACA AAGAGATTAA TAGAATTTTA TGAAGACTAC 
TTTAAAGTGC CCTATTCCTT GCCAAAACTA GATCTTTTAG CTGTGCCTAA GCATCCGTAT 
GCTGCTATGG AGAACTGGGG ACTAAGTATT TTTGTGGAAC AAAGAATACT GCTGGATCCC 
AGTGTTTCAT CTATTTCTTA TTTGCTGGAT GTCACCATGG TCATTGTTCA TGAGATATGT 
CACCAGTGGT TTGGTGACCT TGTGACGCCT GTGTGGTGGG AAGACGTGTG GCTGAAGGAA 
GGGTTTGCTC ACTACTTTGA ATTTGTTGGT ACAGACTACC TCTATCCTGG CTGGAACATG 
GAAAAGCAGA GGTTTCTGAC CGATGTTCTG CATGAAGTGA TGCTGCTGGA CGGTTTGGCC 
AGTTCCCATC CAGTATCACA GGAAGTGCTG CAGGCAACAG ATATTGACAG GGTGTTTGAC 
TGGATCGCAT ATAAAAAGGG TGCTGCTTTA ATAAGAATGC TGGCTAATTT TATGGGCCAT 
TCAGTTTTCC AGAGGGGTTT GCAAGATTAT TTAACCATTC ATAAGTATGG TAATGCAGCC 
AGAAATGATC TCTGGAATAC ATTATCGGAG GCTTTAAAAA GAAATGGGAA ATATGTAAAT 
ATACAAGAAG TAATGGATCA GTGGACACTC CAGATGGGTT ATCCTGTTAT CACCATCTTG 
GGAAACACAA CAGCAGAAAA TAGAATAATA ATTACCCAAC AGCATTTTAT CTATGATATC 
AGTGCTAAAA CTAAAGCACT TAAACTTCAG AATAACAGTT ACCTGTGGCA GATTCCATTA 
ACTATTGTGG TAGGAAATAG AAGCCATGTG TCTTCAGAAG CAATTATTTG GGTGTCTAAC 
AAATCAGAGC ACCACAGAAT AACTTATTTG GACAAAGGAA GCTGGCTGCT GGGGAACATC 
AATCAAACTG GCTATTTTAG AGTCAACTAT GACCTAAGGA ACTGGAGATT ATTAATTGAT 
CAATTAATCC GGAATCATGA GGTTCTTTCT GTCAGTAACC GAGCGGGCTT GATCGATGAT 
GCCTTCAGCC TAGCCAGGGC TGGCTATTTG CCTCAGAATA TTCCTCTGGA GATTATCAGA 
TACCTGTCTG AGGAGAAGGA TTTTCTTCCT TGGCATGCTG CCAGCCGAGC TCTTTATCCT 
CTAGATAAAT TACTGGACCG CATGGAAAAC TACAACATTT TCAATGAATA TATTTTAAAG 
CAAGTTGCAA CAACATATAT CAAGCTTGGG TGGCCGAAAA ATAATTTTAA TGGATCTCTT 
GTTCAAGCAT CCTACCAACA TGAAGAACTA CGTAGAGAAG TTATAATGCT GGCCTGCAGT 
TTTGGCAACA AGCACTGTCA CCAACAGGCA TCAACACTTA TTTCAGATTG GATTTCCAGC 
AACAGGAACA GAATACCACT AAATGTTAGA GACATCGTAT ACTGTACAGG AGTGTCACTA 
CTGGATGAGG ATGTCTGGGA ATTCATATGG ATGAAATTCC ATTCCACCAC AGCAGTTTCT 
GAGAAGAAAA TATTATTGGA AGCCTTAACT TGCAGTGATG ACAGGAATTT ATTAAACAGG 
CTTCTAAATC TGTCACTGAA TTCTGAGGTG GTGCTGGATC AAGATGCAAT TGATGTCATA 
ATCCATGTAG CTCGAAATCC ACATGGTCGA GACCTTGCCT GGAAGTTTTT CAGGGATAAA 
TGGAAGATAT TAAATACCAG GTATGGAGAA GCATTGTTTA TGAATTCCAA ACTCATCAGT 
GGTGTCACAG AATTTCTTAA TACTGAAGGT GAACTCAAAG AGCTCAAGAA CTTCATGAAA 
AACTATGATG GGGTAGCTGC TGCTTCTTTC TCACGAGCTG TGGAAACTGT CGAAGCCAAT 
GTGCGCTGGA AAATGCTTTA CCAAGACGAG CTTTTCCAAT GGTTAGGAAA AGCTCTAAGA 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRHDE ( XM_005268819.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268876.1 Homo sapiens thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu46507
Clone ID Related Accession (Same CDS sequence)	XM_005268819.4
Accession Version	XM_005268819.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	thyrotropin-releasing hormone-degrading ectoenzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005268819.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGG ACGGCGAGCT GGGGGAGCAA GAGGAGGAGA AGAAAAAGAA GAAGAAAAAG 
AAGAGGAAGA AGAAGAAGGA GGAGGAGGAG GAGGAGGAGG GGGCCGAGAA GAGCAGCTCA 
CCCTTCGCAG CCGCGATGGG GGAAGACGAC GCCGCGCTTC GGGCTGGCAG CAGGGGGCTC 
TCCGACCCGT GGGCAGACTC AGTGGGAGTG CGACCCCGCA CCACGGAGCG CCACATCGCC 
GTACACAAGC GGCTTGTGCT GGCCTTCGCT GTGTCCCTCG TGGCATTGCT CGCGGTCACA 
ATGCTCGCTG TGCTGCTCAG CCTGCGCTTC GACGAGTGCG GGGCGAGTGC CACGCCAGGC 
GCCGACGGTG GCCCCTCAGG CTTTCCGGAG CGCGGCGGCA ACGGGAGCCT CCCTGGATCG 
GCCCGGCGCA ACCACCACGC AGGCGGGGAC TCCTGGCAGC CCGAGGCGGG TGGGGTGGCC 
AGTCCGGGGA CCACGTCGGC CCAGCCGCCG TCGGAGGAGG AGCGGGAGCC GTGGGAGCCG 
TGGACGCAGC TGCGCCTGTC GGGCCACCTG AAGCCGCTGC ACTACAATCT GATGCTCACC 
GCCTTCATGG AGAACTTCAC CTTCTCCGGG GAGGTCAACG TGGAGATCGC GTGCCGGAAC 
GCCACCCGCT ACGTAGTGCT GCACGCTTCC CGAGTGGCGG TGGAGAAAGT GCAGCTGGCC 
GAGGACCGGG CGTTCGGGGC TGTCCCTGTA GCCGGTTTTT TCCTCTACCC GCAAACCCAG 
GTCTTAGTGG TGGTGCTGAA TAGGACACTG GACGCGCAGA GGAATTACAA TCTGAAGATT 
ATCTACAACG CGCTCATCGA GAATGAGCTC CTGGGCTTCT TCCGCAGCTC CTATGTGCTC 
CACGGGGAGA GAAGATTCCT TGGTGTTACT CAGTTTTCGC CTACACATGC CAGAAAGGCA 
TTTCCTTGTT TTGATGAGCC AATCTACAAG GCTACTTTCA AAATCAGCAT CAAGCATCAA 
GCAACCTATT TATCTTTATC TAATATGCCA GTGGAAACTT CCGTGTTTGA GGAAGATGGA 
TGGGTTACGG ATCACTTTTC ACAGACCCCT CTCATGTCCA CATATTATTT AGCCTGGGCA 
ATTTGCAACT TCACATACAG AGAAACTACC ACCAAGAGTG GGGTTGTAGT ACGATTATAT 
GCAAGACCTG ATGCTATCAG AAGAGGATCC GGGGACTATG CTCTCCATAT AACAAAGAGA 
TTAATAGAAT TTTATGAAGA CTACTTTAAA GTGCCCTATT CCTTGCCAAA ACTAGATCTT 
TTAGCTGTGC CTAAGCATCC GTATGCTGCT ATGGAGAACT GGGGACTAAG TATTTTTGTG 
GAACAAAGAA TACTGCTGGA TCCCAGTGTT TCATCTATTT CTTATTTGCT GGATGTCACC 
ATGGTCATTG TTCATGAGAT ATGTCACCAG TGGTTTGGTG ACCTTGTGAC GCCTGTGTGG 
TGGGAAGACG TGTGGCTGAA GGAAGGGTTT GCTCACTACT TTGAATTTGT TGGTACAGAC 
TACCTCTATC CTGGCTGGAA CATGGAAAAG CAGAGGTTTC TGACCGATGT TCTGCATGAA 
GTGATGCTGC TGGACGGTTT GGCCAGTTCC CATCCAGTAT CACAGGAAGT GCTGCAGGCA 
ACAGATATTG ACAGGGTGTT TGACTGGATC GCATATAAAA AGGGTGCTGC TTTAATAAGA 
ATGCTGGCTA ATTTTATGGG CCATTCAGTT TTCCAGAGGG GTTTGCAAGA TTATTTAACC 
ATTCATAAGT ATGGTAATGC AGCCAGAAAT GATCTCTGGA ATACATTATC GGAGGCTTTA 
AAAAGAAATG GGAAATATGT AAATATACAA GAAGTAATGG ATCAGTGGAC ACTCCAGATG 
GGTTATCCTG TTATCACCAT CTTGGGAAAC ACAACAGCAG AAAATAGAAT AATAATTACC 
CAACAGCATT TTATCTATGA TATCAGTGCT AAAACTAAAG CACTTAAACT TCAGAATAAC 
AGTTACCTGT GGCAGATTCC ATTAACTATT GTGGTAGGAA ATAGAAGCCA TGTGTCTTCA 
GAAGCAATTA TTTGGGTGTC TAACAAATCA GAGCACCACA GAATAACTTA TTTGGACAAA 
GGAAGCTGGC TGCTGGGGAA CATCAATCAA ACTGGCTATT TTAGAGTCAA CTATGACCTA 
AGGAACTGGA GATTATTAAT TGATCAATTA ATCCGGAATC ATGAGGTTCT TTCTGTCAGT 
AACCGAGCGG GCTTGATCGA TGATGCCTTC AGCCTAGCCA GCTTATACAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268876.1
CDS	1..2334
Translation	MALDGELGEQEEEKKKKKKKKRKKKKEEEEEEEGAEKSSSPFAAAMGEDDAALRAGSRGLSDPWADSVGVRPRTTERHIAVHKRLVLAFAVSLVALLAVTMLAVLLSLRFDECGASATPGADGGPSGFPERGGNGSLPGSARRNHHAGGDSWQPEAGGVASPGTTSAQPPSEEEREPWEPWTQLRLSGHLKPLHYNLMLTAFMENFTFSGEVNVEIACRNATRYVVLHASRVAVEKVQLAEDRAFGAVPVAGFFLYPQTQVLVVVLNRTLDAQRNYNLKIIYNALIENELLGFFRSSYVLHGERRFLGVTQFSPTHARKAFPCFDEPIYKATFKISIKHQATYLSLSNMPVETSVFEEDGWVTDHFSQTPLMSTYYLAWAICNFTYRETTTKSGVVVRLYARPDAIRRGSGDYALHITKRLIEFYEDYFKVPYSLPKLDLLAVPKHPYAAMENWGLSIFVEQRILLDPSVSSISYLLDVTMVIVHEICHQWFGDLVTPVWWEDVWLKEGFAHYFEFVGTDYLYPGWNMEKQRFLTDVLHEVMLLDGLASSHPVSQEVLQATDIDRVFDWIAYKKGAALIRMLANFMGHSVFQRGLQDYLTIHKYGNAARNDLWNTLSEALKRNGKYVNIQEVMDQWTLQMGYPVITILGNTTAENRIIITQQHFIYDISAKTKALKLQNNSYLWQIPLTIVVGNRSHVSSEAIIWVSNKSEHHRITYLDKGSWLLGNINQTGYFRVNYDLRNWRLLIDQLIRNHEVLSVSNRAGLIDDAFSLASLYI

Target ORF information:

RefSeq Version	XM_005268819.4
Organism	Homo sapiens(human)
Definition	Homo sapiens thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268819.4

ORF Insert Sequence:

ATGGCCCTGG ACGGCGAGCT GGGGGAGCAA GAGGAGGAGA AGAAAAAGAA GAAGAAAAAG 
AAGAGGAAGA AGAAGAAGGA GGAGGAGGAG GAGGAGGAGG GGGCCGAGAA GAGCAGCTCA 
CCCTTCGCAG CCGCGATGGG GGAAGACGAC GCCGCGCTTC GGGCTGGCAG CAGGGGGCTC 
TCCGACCCGT GGGCAGACTC AGTGGGAGTG CGACCCCGCA CCACGGAGCG CCACATCGCC 
GTACACAAGC GGCTTGTGCT GGCCTTCGCT GTGTCCCTCG TGGCATTGCT CGCGGTCACA 
ATGCTCGCTG TGCTGCTCAG CCTGCGCTTC GACGAGTGCG GGGCGAGTGC CACGCCAGGC 
GCCGACGGTG GCCCCTCAGG CTTTCCGGAG CGCGGCGGCA ACGGGAGCCT CCCTGGATCG 
GCCCGGCGCA ACCACCACGC AGGCGGGGAC TCCTGGCAGC CCGAGGCGGG TGGGGTGGCC 
AGTCCGGGGA CCACGTCGGC CCAGCCGCCG TCGGAGGAGG AGCGGGAGCC GTGGGAGCCG 
TGGACGCAGC TGCGCCTGTC GGGCCACCTG AAGCCGCTGC ACTACAATCT GATGCTCACC 
GCCTTCATGG AGAACTTCAC CTTCTCCGGG GAGGTCAACG TGGAGATCGC GTGCCGGAAC 
GCCACCCGCT ACGTAGTGCT GCACGCTTCC CGAGTGGCGG TGGAGAAAGT GCAGCTGGCC 
GAGGACCGGG CGTTCGGGGC TGTCCCTGTA GCCGGTTTTT TCCTCTACCC GCAAACCCAG 
GTCTTAGTGG TGGTGCTGAA TAGGACACTG GACGCGCAGA GGAATTACAA TCTGAAGATT 
ATCTACAACG CGCTCATCGA GAATGAGCTC CTGGGCTTCT TCCGCAGCTC CTATGTGCTC 
CACGGGGAGA GAAGATTCCT TGGTGTTACT CAGTTTTCGC CTACACATGC CAGAAAGGCA 
TTTCCTTGTT TTGATGAGCC AATCTACAAG GCTACTTTCA AAATCAGCAT CAAGCATCAA 
GCAACCTATT TATCTTTATC TAATATGCCA GTGGAAACTT CCGTGTTTGA GGAAGATGGA 
TGGGTTACGG ATCACTTTTC ACAGACCCCT CTCATGTCCA CATATTATTT AGCCTGGGCA 
ATTTGCAACT TCACATACAG AGAAACTACC ACCAAGAGTG GGGTTGTAGT ACGATTATAT 
GCAAGACCTG ATGCTATCAG AAGAGGATCC GGGGACTATG CTCTCCATAT AACAAAGAGA 
TTAATAGAAT TTTATGAAGA CTACTTTAAA GTGCCCTATT CCTTGCCAAA ACTAGATCTT 
TTAGCTGTGC CTAAGCATCC GTATGCTGCT ATGGAGAACT GGGGACTAAG TATTTTTGTG 
GAACAAAGAA TACTGCTGGA TCCCAGTGTT TCATCTATTT CTTATTTGCT GGATGTCACC 
ATGGTCATTG TTCATGAGAT ATGTCACCAG TGGTTTGGTG ACCTTGTGAC GCCTGTGTGG 
TGGGAAGACG TGTGGCTGAA GGAAGGGTTT GCTCACTACT TTGAATTTGT TGGTACAGAC 
TACCTCTATC CTGGCTGGAA CATGGAAAAG CAGAGGTTTC TGACCGATGT TCTGCATGAA 
GTGATGCTGC TGGACGGTTT GGCCAGTTCC CATCCAGTAT CACAGGAAGT GCTGCAGGCA 
ACAGATATTG ACAGGGTGTT TGACTGGATC GCATATAAAA AGGGTGCTGC TTTAATAAGA 
ATGCTGGCTA ATTTTATGGG CCATTCAGTT TTCCAGAGGG GTTTGCAAGA TTATTTAACC 
ATTCATAAGT ATGGTAATGC AGCCAGAAAT GATCTCTGGA ATACATTATC GGAGGCTTTA 
AAAAGAAATG GGAAATATGT AAATATACAA GAAGTAATGG ATCAGTGGAC ACTCCAGATG 
GGTTATCCTG TTATCACCAT CTTGGGAAAC ACAACAGCAG AAAATAGAAT AATAATTACC 
CAACAGCATT TTATCTATGA TATCAGTGCT AAAACTAAAG CACTTAAACT TCAGAATAAC 
AGTTACCTGT GGCAGATTCC ATTAACTATT GTGGTAGGAA ATAGAAGCCA TGTGTCTTCA 
GAAGCAATTA TTTGGGTGTC TAACAAATCA GAGCACCACA GAATAACTTA TTTGGACAAA 
GGAAGCTGGC TGCTGGGGAA CATCAATCAA ACTGGCTATT TTAGAGTCAA CTATGACCTA 
AGGAACTGGA GATTATTAAT TGATCAATTA ATCCGGAATC ATGAGGTTCT TTCTGTCAGT 
AACCGAGCGG GCTTGATCGA TGATGCCTTC AGCCTAGCCA GCTTATACAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRHDE ( XM_017019243.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874732.2 Homo sapiens thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu103716
Clone ID Related Accession (Same CDS sequence)	XM_017019243.2
Accession Version	XM_017019243.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	thyrotropin-releasing hormone-degrading ectoenzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017019243.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAAG ACGACGCCGC GCTTCGGGCT GGCAGCAGGG GGCTCTCCGA CCCGTGGGCA 
GACTCAGTGG GAGTGCGACC CCGCACCACG GAGCGCCACA TCGCCGTACA CAAGCGGCTT 
GTGCTGGCCT TCGCTGTGTC CCTCGTGGCA TTGCTCGCGG TCACAATGCT CGCTGTGCTG 
CTCAGCCTGC GCTTCGACGA GTGCGGGGCG AGTGCCACGC CAGGCGCCGA CGGTGGCCCC 
TCAGGCTTTC CGGAGCGCGG CGGCAACGGG AGCCTCCCTG GATCGGCCCG GCGCAACCAC 
CACGCAGGCG GGGACTCCTG GCAGCCCGAG GCGGGTGGGG TGGCCAGTCC GGGGACCACG 
TCGGCCCAGC CGCCGTCGGA GGAGGAGCGG GAGCCGTGGG AGCCGTGGAC GCAGCTGCGC 
CTGTCGGGCC ACCTGAAGCC GCTGCACTAC AATCTGATGC TCACCGCCTT CATGGAGAAC 
TTCACCTTCT CCGGGGAGGT CAACGTGGAG ATCGCGTGCC GGAACGCCAC CCGCTACGTA 
GTGCTGCACG CTTCCCGAGT GGCGGTGGAG AAAGTGCAGC TGGCCGAGGA CCGGGCGTTC 
GGGGCTGTCC CTGTAGCCGG TTTTTTCCTC TACCCGCAAA CCCAGGTCTT AGTGGTGGTG 
CTGAATAGGA CACTGGACGC GCAGAGGAAT TACAATCTGA AGATTATCTA CAACGCGCTC 
ATCGAGAATG AGCTCCTGGG CTTCTTCCGC AGCTCCTATG TGCTCCACGG GGAGAGAAGA 
TTCCTTGGTG TTACTCAGTT TTCGCCTACA CATGCCAGAA AGGCATTTCC TTGTTTTGAT 
GAGCCAATCT ACAAGGCTAC TTTCAAAATC AGCATCAAGC ATCAAGCAAC CTATTTATCT 
TTATCTAATA TGCCAGTGGA AACTTCCGTG TTTGAGGAAG ATGGATGGGT TACGGATCAC 
TTTTCACAGA CCCCTCTCAT GTCCACATAT TATTTAGCCT GGGCAATTTG CAACTTCACA 
TACAGAGAAA CTACCACCAA GAGTGGGGTT GTAGTACGAT TATATGCAAG ACCTGATGCT 
ATCAGAAGAG GATCCGGGGA CTATGCTCTC CATATAACAA AGAGATTAAT AGAATTTTAT 
GAAGACTACT TTAAAGTGCC CTATTCCTTG CCAAAACTAG ATCTTTTAGC TGTGCCTAAG 
CATCCGTATG CTGCTATGGA GAACTGGGGA CTAAGTATTT TTGTGGAACA AAGAATACTG 
CTGGATCCCA GTGTTTCATC TATTTCTTAT TTGCTGGATG TCACCATGGT CATTGTTCAT 
GAGATATGTC ACCAGTGGTT TGGTGACCTT GTGACGCCTG TGTGGTGGGA AGACGTGTGG 
CTGAAGGAAG GGTTTGCTCA CTACTTTGAA TTTGTTGGTA CAGACTACCT CTATCCTGGC 
TGGAACATGG AAAAGCAGAG GTTTCTGACC GATGTTCTGC ATGAAGTGAT GCTGCTGGAC 
GGTTTGGCCA GTTCCCATCC AGTATCACAG GAAGTGCTGC AGGCAACAGA TATTGACAGG 
GTGTTTGACT GGATCGCATA TAAAAAGGGT GCTGCTTTAA TAAGAATGCT GGCTAATTTT 
ATGGGCCATT CAGTTTTCCA GAGGGGTTTG CAAGATTATT TAACCATTCA TAAGTATGGT 
AATGCAGCCA GAAATGATCT CTGGAATACA TTATCGGAGG CTTTAAAAAG AAATGGGAAA 
TATGTAAATA TACAAGAAGT AATGGATCAG TGGACACTCC AGATGGGTTA TCCTGTTATC 
ACCATCTTGG GAAACACAAC AGCAGAAAAT AGAATAATAA TTACCCAACA GCATTTTATC 
TATGATATCA GTGCTAAAAC TAAAGCACTT AAACTTCAGA ATAACAGTTA CCTGTGGCAG 
ATTCCATTAA CTATTGTGGT AGGAAATAGA AGCCATGTGT CTTCAGAAGC AATTATTTGG 
GTGTCTAACA AATCAGAGCA CCACAGAATA ACTTATTTGG ACAAAGGAAG CTGGCTGCTG 
GGGAACATCA ATCAAACTGG CTATTTTAGA GTCAACTATG ACCTAAGGAA CTGGAGATTA 
TTAATTGATC AATTAATCCG GAATCATGAG GTTCTTTCTG TCAGTAACCG AGCGGGCTTG 
ATCGATGATG CCTTCAGCCT AGCCAGGGCT GGCTATTTGC CTCAGAATAT TCCTCTGGAG 
ATTATCAGAT ACCTGTCTGA GGAGAAGGAT TTTCTTCCTT GGCATGCTGC CAGCCGAGCT 
CTTTATCCTC TAGATAAATT ACTGGACCGC ATGGAAAACT ACAACATTTT CAATGAATAT 
ATTTTAAAGC AAGTTGCAAC AACATATATC AAGCTTGGGT GGCCGAAAAA TAATTTTAAT 
GGATCTCTTG TTCAAGCATC CTACCAACAT GAAGAACTAC GTAGAGAAGT TATAATGCTG 
GCCTGCAGTT TTGGCAACAA GCACTGTCAC CAACAGGCAT CAACACTTAT TTCAGATTGG 
ATTTCCAGCA ACAGGAACAG AATACCACTA AATGTTAGAG ACATCGTATA CTGTACAGGA 
GTGTCACTAC TGGATGAGGA TGTCTGGGAA TTCATATGGA TGAAATTCCA TTCCACCACA 
GCAGTTTCTG AGAAGAAAAT ATTATTGGAA GCCTTAACTT GCAGTGATGA CAGGAATTTA 
TTAAACAGGC TTCTAAATCT GTCACTGAAT TCTGAGGTGG TGCTGGATCA AGATGCAATT 
GATGTCATAA TCCATGTAGC TCGAAATCCA CATGGTCGAG ACCTTGCCTG GAAGTTTTTC 
AGGGATAAAT GGAAGATATT AAATACCAGT TGCAGTAAAA TTTACTCCCA GGACATCTTG 
TTTCATGGTG AGAATTCTTT AGCCTCAATT TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874732.2
CDS	15..2930
Translation	MGEDDAALRAGSRGLSDPWADSVGVRPRTTERHIAVHKRLVLAFAVSLVALLAVTMLAVLLSLRFDECGASATPGADGGPSGFPERGGNGSLPGSARRNHHAGGDSWQPEAGGVASPGTTSAQPPSEEEREPWEPWTQLRLSGHLKPLHYNLMLTAFMENFTFSGEVNVEIACRNATRYVVLHASRVAVEKVQLAEDRAFGAVPVAGFFLYPQTQVLVVVLNRTLDAQRNYNLKIIYNALIENELLGFFRSSYVLHGERRFLGVTQFSPTHARKAFPCFDEPIYKATFKISIKHQATYLSLSNMPVETSVFEEDGWVTDHFSQTPLMSTYYLAWAICNFTYRETTTKSGVVVRLYARPDAIRRGSGDYALHITKRLIEFYEDYFKVPYSLPKLDLLAVPKHPYAAMENWGLSIFVEQRILLDPSVSSISYLLDVTMVIVHEICHQWFGDLVTPVWWEDVWLKEGFAHYFEFVGTDYLYPGWNMEKQRFLTDVLHEVMLLDGLASSHPVSQEVLQATDIDRVFDWIAYKKGAALIRMLANFMGHSVFQRGLQDYLTIHKYGNAARNDLWNTLSEALKRNGKYVNIQEVMDQWTLQMGYPVITILGNTTAENRIIITQQHFIYDISAKTKALKLQNNSYLWQIPLTIVVGNRSHVSSEAIIWVSNKSEHHRITYLDKGSWLLGNINQTGYFRVNYDLRNWRLLIDQLIRNHEVLSVSNRAGLIDDAFSLARAGYLPQNIPLEIIRYLSEEKDFLPWHAASRALYPLDKLLDRMENYNIFNEYILKQVATTYIKLGWPKNNFNGSLVQASYQHEELRREVIMLACSFGNKHCHQQASTLISDWISSNRNRIPLNVRDIVYCTGVSLLDEDVWEFIWMKFHSTTAVSEKKILLEALTCSDDRNLLNRLLNLSLNSEVVLDQDAIDVIIHVARNPHGRDLAWKFFRDKWKILNTSCSKIYSQDILFHGENSLASIL

Target ORF information:

RefSeq Version	XM_017019243.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019243.2

ORF Insert Sequence:

ATGGGGGAAG ACGACGCCGC GCTTCGGGCT GGCAGCAGGG GGCTCTCCGA CCCGTGGGCA 
GACTCAGTGG GAGTGCGACC CCGCACCACG GAGCGCCACA TCGCCGTACA CAAGCGGCTT 
GTGCTGGCCT TCGCTGTGTC CCTCGTGGCA TTGCTCGCGG TCACAATGCT CGCTGTGCTG 
CTCAGCCTGC GCTTCGACGA GTGCGGGGCG AGTGCCACGC CAGGCGCCGA CGGTGGCCCC 
TCAGGCTTTC CGGAGCGCGG CGGCAACGGG AGCCTCCCTG GATCGGCCCG GCGCAACCAC 
CACGCAGGCG GGGACTCCTG GCAGCCCGAG GCGGGTGGGG TGGCCAGTCC GGGGACCACG 
TCGGCCCAGC CGCCGTCGGA GGAGGAGCGG GAGCCGTGGG AGCCGTGGAC GCAGCTGCGC 
CTGTCGGGCC ACCTGAAGCC GCTGCACTAC AATCTGATGC TCACCGCCTT CATGGAGAAC 
TTCACCTTCT CCGGGGAGGT CAACGTGGAG ATCGCGTGCC GGAACGCCAC CCGCTACGTA 
GTGCTGCACG CTTCCCGAGT GGCGGTGGAG AAAGTGCAGC TGGCCGAGGA CCGGGCGTTC 
GGGGCTGTCC CTGTAGCCGG TTTTTTCCTC TACCCGCAAA CCCAGGTCTT AGTGGTGGTG 
CTGAATAGGA CACTGGACGC GCAGAGGAAT TACAATCTGA AGATTATCTA CAACGCGCTC 
ATCGAGAATG AGCTCCTGGG CTTCTTCCGC AGCTCCTATG TGCTCCACGG GGAGAGAAGA 
TTCCTTGGTG TTACTCAGTT TTCGCCTACA CATGCCAGAA AGGCATTTCC TTGTTTTGAT 
GAGCCAATCT ACAAGGCTAC TTTCAAAATC AGCATCAAGC ATCAAGCAAC CTATTTATCT 
TTATCTAATA TGCCAGTGGA AACTTCCGTG TTTGAGGAAG ATGGATGGGT TACGGATCAC 
TTTTCACAGA CCCCTCTCAT GTCCACATAT TATTTAGCCT GGGCAATTTG CAACTTCACA 
TACAGAGAAA CTACCACCAA GAGTGGGGTT GTAGTACGAT TATATGCAAG ACCTGATGCT 
ATCAGAAGAG GATCCGGGGA CTATGCTCTC CATATAACAA AGAGATTAAT AGAATTTTAT 
GAAGACTACT TTAAAGTGCC CTATTCCTTG CCAAAACTAG ATCTTTTAGC TGTGCCTAAG 
CATCCGTATG CTGCTATGGA GAACTGGGGA CTAAGTATTT TTGTGGAACA AAGAATACTG 
CTGGATCCCA GTGTTTCATC TATTTCTTAT TTGCTGGATG TCACCATGGT CATTGTTCAT 
GAGATATGTC ACCAGTGGTT TGGTGACCTT GTGACGCCTG TGTGGTGGGA AGACGTGTGG 
CTGAAGGAAG GGTTTGCTCA CTACTTTGAA TTTGTTGGTA CAGACTACCT CTATCCTGGC 
TGGAACATGG AAAAGCAGAG GTTTCTGACC GATGTTCTGC ATGAAGTGAT GCTGCTGGAC 
GGTTTGGCCA GTTCCCATCC AGTATCACAG GAAGTGCTGC AGGCAACAGA TATTGACAGG 
GTGTTTGACT GGATCGCATA TAAAAAGGGT GCTGCTTTAA TAAGAATGCT GGCTAATTTT 
ATGGGCCATT CAGTTTTCCA GAGGGGTTTG CAAGATTATT TAACCATTCA TAAGTATGGT 
AATGCAGCCA GAAATGATCT CTGGAATACA TTATCGGAGG CTTTAAAAAG AAATGGGAAA 
TATGTAAATA TACAAGAAGT AATGGATCAG TGGACACTCC AGATGGGTTA TCCTGTTATC 
ACCATCTTGG GAAACACAAC AGCAGAAAAT AGAATAATAA TTACCCAACA GCATTTTATC 
TATGATATCA GTGCTAAAAC TAAAGCACTT AAACTTCAGA ATAACAGTTA CCTGTGGCAG 
ATTCCATTAA CTATTGTGGT AGGAAATAGA AGCCATGTGT CTTCAGAAGC AATTATTTGG 
GTGTCTAACA AATCAGAGCA CCACAGAATA ACTTATTTGG ACAAAGGAAG CTGGCTGCTG 
GGGAACATCA ATCAAACTGG CTATTTTAGA GTCAACTATG ACCTAAGGAA CTGGAGATTA 
TTAATTGATC AATTAATCCG GAATCATGAG GTTCTTTCTG TCAGTAACCG AGCGGGCTTG 
ATCGATGATG CCTTCAGCCT AGCCAGGGCT GGCTATTTGC CTCAGAATAT TCCTCTGGAG 
ATTATCAGAT ACCTGTCTGA GGAGAAGGAT TTTCTTCCTT GGCATGCTGC CAGCCGAGCT 
CTTTATCCTC TAGATAAATT ACTGGACCGC ATGGAAAACT ACAACATTTT CAATGAATAT 
ATTTTAAAGC AAGTTGCAAC AACATATATC AAGCTTGGGT GGCCGAAAAA TAATTTTAAT 
GGATCTCTTG TTCAAGCATC CTACCAACAT GAAGAACTAC GTAGAGAAGT TATAATGCTG 
GCCTGCAGTT TTGGCAACAA GCACTGTCAC CAACAGGCAT CAACACTTAT TTCAGATTGG 
ATTTCCAGCA ACAGGAACAG AATACCACTA AATGTTAGAG ACATCGTATA CTGTACAGGA 
GTGTCACTAC TGGATGAGGA TGTCTGGGAA TTCATATGGA TGAAATTCCA TTCCACCACA 
GCAGTTTCTG AGAAGAAAAT ATTATTGGAA GCCTTAACTT GCAGTGATGA CAGGAATTTA 
TTAAACAGGC TTCTAAATCT GTCACTGAAT TCTGAGGTGG TGCTGGATCA AGATGCAATT 
GATGTCATAA TCCATGTAGC TCGAAATCCA CATGGTCGAG ACCTTGCCTG GAAGTTTTTC 
AGGGATAAAT GGAAGATATT AAATACCAGT TGCAGTAAAA TTTACTCCCA GGACATCTTG 
TTTCATGGTG AGAATTCTTT AGCCTCAATT TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRHDE ( XM_005268819.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268876.2 Homo sapiens thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu103717
Clone ID Related Accession (Same CDS sequence)	XM_005268819.5
Accession Version	XM_005268819.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	thyrotropin-releasing hormone-degrading ectoenzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005268819.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAAG ACGACGCCGC GCTTCGGGCT GGCAGCAGGG GGCTCTCCGA CCCGTGGGCA 
GACTCAGTGG GAGTGCGACC CCGCACCACG GAGCGCCACA TCGCCGTACA CAAGCGGCTT 
GTGCTGGCCT TCGCTGTGTC CCTCGTGGCA TTGCTCGCGG TCACAATGCT CGCTGTGCTG 
CTCAGCCTGC GCTTCGACGA GTGCGGGGCG AGTGCCACGC CAGGCGCCGA CGGTGGCCCC 
TCAGGCTTTC CGGAGCGCGG CGGCAACGGG AGCCTCCCTG GATCGGCCCG GCGCAACCAC 
CACGCAGGCG GGGACTCCTG GCAGCCCGAG GCGGGTGGGG TGGCCAGTCC GGGGACCACG 
TCGGCCCAGC CGCCGTCGGA GGAGGAGCGG GAGCCGTGGG AGCCGTGGAC GCAGCTGCGC 
CTGTCGGGCC ACCTGAAGCC GCTGCACTAC AATCTGATGC TCACCGCCTT CATGGAGAAC 
TTCACCTTCT CCGGGGAGGT CAACGTGGAG ATCGCGTGCC GGAACGCCAC CCGCTACGTA 
GTGCTGCACG CTTCCCGAGT GGCGGTGGAG AAAGTGCAGC TGGCCGAGGA CCGGGCGTTC 
GGGGCTGTCC CTGTAGCCGG TTTTTTCCTC TACCCGCAAA CCCAGGTCTT AGTGGTGGTG 
CTGAATAGGA CACTGGACGC GCAGAGGAAT TACAATCTGA AGATTATCTA CAACGCGCTC 
ATCGAGAATG AGCTCCTGGG CTTCTTCCGC AGCTCCTATG TGCTCCACGG GGAGAGAAGA 
TTCCTTGGTG TTACTCAGTT TTCGCCTACA CATGCCAGAA AGGCATTTCC TTGTTTTGAT 
GAGCCAATCT ACAAGGCTAC TTTCAAAATC AGCATCAAGC ATCAAGCAAC CTATTTATCT 
TTATCTAATA TGCCAGTGGA AACTTCCGTG TTTGAGGAAG ATGGATGGGT TACGGATCAC 
TTTTCACAGA CCCCTCTCAT GTCCACATAT TATTTAGCCT GGGCAATTTG CAACTTCACA 
TACAGAGAAA CTACCACCAA GAGTGGGGTT GTAGTACGAT TATATGCAAG ACCTGATGCT 
ATCAGAAGAG GATCCGGGGA CTATGCTCTC CATATAACAA AGAGATTAAT AGAATTTTAT 
GAAGACTACT TTAAAGTGCC CTATTCCTTG CCAAAACTAG ATCTTTTAGC TGTGCCTAAG 
CATCCGTATG CTGCTATGGA GAACTGGGGA CTAAGTATTT TTGTGGAACA AAGAATACTG 
CTGGATCCCA GTGTTTCATC TATTTCTTAT TTGCTGGATG TCACCATGGT CATTGTTCAT 
GAGATATGTC ACCAGTGGTT TGGTGACCTT GTGACGCCTG TGTGGTGGGA AGACGTGTGG 
CTGAAGGAAG GGTTTGCTCA CTACTTTGAA TTTGTTGGTA CAGACTACCT CTATCCTGGC 
TGGAACATGG AAAAGCAGAG GTTTCTGACC GATGTTCTGC ATGAAGTGAT GCTGCTGGAC 
GGTTTGGCCA GTTCCCATCC AGTATCACAG GAAGTGCTGC AGGCAACAGA TATTGACAGG 
GTGTTTGACT GGATCGCATA TAAAAAGGGT GCTGCTTTAA TAAGAATGCT GGCTAATTTT 
ATGGGCCATT CAGTTTTCCA GAGGGGTTTG CAAGATTATT TAACCATTCA TAAGTATGGT 
AATGCAGCCA GAAATGATCT CTGGAATACA TTATCGGAGG CTTTAAAAAG AAATGGGAAA 
TATGTAAATA TACAAGAAGT AATGGATCAG TGGACACTCC AGATGGGTTA TCCTGTTATC 
ACCATCTTGG GAAACACAAC AGCAGAAAAT AGAATAATAA TTACCCAACA GCATTTTATC 
TATGATATCA GTGCTAAAAC TAAAGCACTT AAACTTCAGA ATAACAGTTA CCTGTGGCAG 
ATTCCATTAA CTATTGTGGT AGGAAATAGA AGCCATGTGT CTTCAGAAGC AATTATTTGG 
GTGTCTAACA AATCAGAGCA CCACAGAATA ACTTATTTGG ACAAAGGAAG CTGGCTGCTG 
GGGAACATCA ATCAAACTGG CTATTTTAGA GTCAACTATG ACCTAAGGAA CTGGAGATTA 
TTAATTGATC AATTAATCCG GAATCATGAG GTTCTTTCTG TCAGTAACCG AGCGGGCTTG 
ATCGATGATG CCTTCAGCCT AGCCAGCTTA TACATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268876.2
CDS	15..2213
Translation	MGEDDAALRAGSRGLSDPWADSVGVRPRTTERHIAVHKRLVLAFAVSLVALLAVTMLAVLLSLRFDECGASATPGADGGPSGFPERGGNGSLPGSARRNHHAGGDSWQPEAGGVASPGTTSAQPPSEEEREPWEPWTQLRLSGHLKPLHYNLMLTAFMENFTFSGEVNVEIACRNATRYVVLHASRVAVEKVQLAEDRAFGAVPVAGFFLYPQTQVLVVVLNRTLDAQRNYNLKIIYNALIENELLGFFRSSYVLHGERRFLGVTQFSPTHARKAFPCFDEPIYKATFKISIKHQATYLSLSNMPVETSVFEEDGWVTDHFSQTPLMSTYYLAWAICNFTYRETTTKSGVVVRLYARPDAIRRGSGDYALHITKRLIEFYEDYFKVPYSLPKLDLLAVPKHPYAAMENWGLSIFVEQRILLDPSVSSISYLLDVTMVIVHEICHQWFGDLVTPVWWEDVWLKEGFAHYFEFVGTDYLYPGWNMEKQRFLTDVLHEVMLLDGLASSHPVSQEVLQATDIDRVFDWIAYKKGAALIRMLANFMGHSVFQRGLQDYLTIHKYGNAARNDLWNTLSEALKRNGKYVNIQEVMDQWTLQMGYPVITILGNTTAENRIIITQQHFIYDISAKTKALKLQNNSYLWQIPLTIVVGNRSHVSSEAIIWVSNKSEHHRITYLDKGSWLLGNINQTGYFRVNYDLRNWRLLIDQLIRNHEVLSVSNRAGLIDDAFSLASLYI

Target ORF information:

RefSeq Version	XM_005268819.5
Organism	Homo sapiens(human)
Definition	Homo sapiens thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268819.5

ORF Insert Sequence:

ATGGGGGAAG ACGACGCCGC GCTTCGGGCT GGCAGCAGGG GGCTCTCCGA CCCGTGGGCA 
GACTCAGTGG GAGTGCGACC CCGCACCACG GAGCGCCACA TCGCCGTACA CAAGCGGCTT 
GTGCTGGCCT TCGCTGTGTC CCTCGTGGCA TTGCTCGCGG TCACAATGCT CGCTGTGCTG 
CTCAGCCTGC GCTTCGACGA GTGCGGGGCG AGTGCCACGC CAGGCGCCGA CGGTGGCCCC 
TCAGGCTTTC CGGAGCGCGG CGGCAACGGG AGCCTCCCTG GATCGGCCCG GCGCAACCAC 
CACGCAGGCG GGGACTCCTG GCAGCCCGAG GCGGGTGGGG TGGCCAGTCC GGGGACCACG 
TCGGCCCAGC CGCCGTCGGA GGAGGAGCGG GAGCCGTGGG AGCCGTGGAC GCAGCTGCGC 
CTGTCGGGCC ACCTGAAGCC GCTGCACTAC AATCTGATGC TCACCGCCTT CATGGAGAAC 
TTCACCTTCT CCGGGGAGGT CAACGTGGAG ATCGCGTGCC GGAACGCCAC CCGCTACGTA 
GTGCTGCACG CTTCCCGAGT GGCGGTGGAG AAAGTGCAGC TGGCCGAGGA CCGGGCGTTC 
GGGGCTGTCC CTGTAGCCGG TTTTTTCCTC TACCCGCAAA CCCAGGTCTT AGTGGTGGTG 
CTGAATAGGA CACTGGACGC GCAGAGGAAT TACAATCTGA AGATTATCTA CAACGCGCTC 
ATCGAGAATG AGCTCCTGGG CTTCTTCCGC AGCTCCTATG TGCTCCACGG GGAGAGAAGA 
TTCCTTGGTG TTACTCAGTT TTCGCCTACA CATGCCAGAA AGGCATTTCC TTGTTTTGAT 
GAGCCAATCT ACAAGGCTAC TTTCAAAATC AGCATCAAGC ATCAAGCAAC CTATTTATCT 
TTATCTAATA TGCCAGTGGA AACTTCCGTG TTTGAGGAAG ATGGATGGGT TACGGATCAC 
TTTTCACAGA CCCCTCTCAT GTCCACATAT TATTTAGCCT GGGCAATTTG CAACTTCACA 
TACAGAGAAA CTACCACCAA GAGTGGGGTT GTAGTACGAT TATATGCAAG ACCTGATGCT 
ATCAGAAGAG GATCCGGGGA CTATGCTCTC CATATAACAA AGAGATTAAT AGAATTTTAT 
GAAGACTACT TTAAAGTGCC CTATTCCTTG CCAAAACTAG ATCTTTTAGC TGTGCCTAAG 
CATCCGTATG CTGCTATGGA GAACTGGGGA CTAAGTATTT TTGTGGAACA AAGAATACTG 
CTGGATCCCA GTGTTTCATC TATTTCTTAT TTGCTGGATG TCACCATGGT CATTGTTCAT 
GAGATATGTC ACCAGTGGTT TGGTGACCTT GTGACGCCTG TGTGGTGGGA AGACGTGTGG 
CTGAAGGAAG GGTTTGCTCA CTACTTTGAA TTTGTTGGTA CAGACTACCT CTATCCTGGC 
TGGAACATGG AAAAGCAGAG GTTTCTGACC GATGTTCTGC ATGAAGTGAT GCTGCTGGAC 
GGTTTGGCCA GTTCCCATCC AGTATCACAG GAAGTGCTGC AGGCAACAGA TATTGACAGG 
GTGTTTGACT GGATCGCATA TAAAAAGGGT GCTGCTTTAA TAAGAATGCT GGCTAATTTT 
ATGGGCCATT CAGTTTTCCA GAGGGGTTTG CAAGATTATT TAACCATTCA TAAGTATGGT 
AATGCAGCCA GAAATGATCT CTGGAATACA TTATCGGAGG CTTTAAAAAG AAATGGGAAA 
TATGTAAATA TACAAGAAGT AATGGATCAG TGGACACTCC AGATGGGTTA TCCTGTTATC 
ACCATCTTGG GAAACACAAC AGCAGAAAAT AGAATAATAA TTACCCAACA GCATTTTATC 
TATGATATCA GTGCTAAAAC TAAAGCACTT AAACTTCAGA ATAACAGTTA CCTGTGGCAG 
ATTCCATTAA CTATTGTGGT AGGAAATAGA AGCCATGTGT CTTCAGAAGC AATTATTTGG 
GTGTCTAACA AATCAGAGCA CCACAGAATA ACTTATTTGG ACAAAGGAAG CTGGCTGCTG 
GGGAACATCA ATCAAACTGG CTATTTTAGA GTCAACTATG ACCTAAGGAA CTGGAGATTA 
TTAATTGATC AATTAATCCG GAATCATGAG GTTCTTTCTG TCAGTAACCG AGCGGGCTTG 
ATCGATGATG CCTTCAGCCT AGCCAGCTTA TACATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRHDE ( XM_017019243.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874732.1 Homo sapiens thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X1, mRNA.

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CloneID	OHu83742
Clone ID Related Accession (Same CDS sequence)	XM_017019243.1
Accession Version	XM_017019243.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	thyrotropin-releasing hormone-degrading ectoenzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGG ACGGCGAGCT GGGGGAGCAA GAGGAGGAGA AGAAAAAGAA GAAGAAAAAG 
AAGAGGAAGA AGAAGAAGGA GGAGGAGGAG GAGGAGGAGG GGGCCGAGAA GAGCAGCTCA 
CCCTTCGCAG CCGCGATGGG GGAAGACGAC GCCGCGCTTC GGGCTGGCAG CAGGGGGCTC 
TCCGACCCGT GGGCAGACTC AGTGGGAGTG CGACCCCGCA CCACGGAGCG CCACATCGCC 
GTACACAAGC GGCTTGTGCT GGCCTTCGCT GTGTCCCTCG TGGCATTGCT CGCGGTCACA 
ATGCTCGCTG TGCTGCTCAG CCTGCGCTTC GACGAGTGCG GGGCGAGTGC CACGCCAGGC 
GCCGACGGTG GCCCCTCAGG CTTTCCGGAG CGCGGCGGCA ACGGGAGCCT CCCTGGATCG 
GCCCGGCGCA ACCACCACGC AGGCGGGGAC TCCTGGCAGC CCGAGGCGGG TGGGGTGGCC 
AGTCCGGGGA CCACGTCGGC CCAGCCGCCG TCGGAGGAGG AGCGGGAGCC GTGGGAGCCG 
TGGACGCAGC TGCGCCTGTC GGGCCACCTG AAGCCGCTGC ACTACAATCT GATGCTCACC 
GCCTTCATGG AGAACTTCAC CTTCTCCGGG GAGGTCAACG TGGAGATCGC GTGCCGGAAC 
GCCACCCGCT ACGTAGTGCT GCACGCTTCC CGAGTGGCGG TGGAGAAAGT GCAGCTGGCC 
GAGGACCGGG CGTTCGGGGC TGTCCCTGTA GCCGGTTTTT TCCTCTACCC GCAAACCCAG 
GTCTTAGTGG TGGTGCTGAA TAGGACACTG GACGCGCAGA GGAATTACAA TCTGAAGATT 
ATCTACAACG CGCTCATCGA GAATGAGCTC CTGGGCTTCT TCCGCAGCTC CTATGTGCTC 
CACGGGGAGA GAAGATTCCT TGGTGTTACT CAGTTTTCGC CTACACATGC CAGAAAGGCA 
TTTCCTTGTT TTGATGAGCC AATCTACAAG GCTACTTTCA AAATCAGCAT CAAGCATCAA 
GCAACCTATT TATCTTTATC TAATATGCCA GTGGAAACTT CCGTGTTTGA GGAAGATGGA 
TGGGTTACGG ATCACTTTTC ACAGACCCCT CTCATGTCCA CATATTATTT AGCCTGGGCA 
ATTTGCAACT TCACATACAG AGAAACTACC ACCAAGAGTG GGGTTGTAGT ACGATTATAT 
GCAAGACCTG ATGCTATCAG AAGAGGATCC GGGGACTATG CTCTCCATAT AACAAAGAGA 
TTAATAGAAT TTTATGAAGA CTACTTTAAA GTGCCCTATT CCTTGCCAAA ACTAGATCTT 
TTAGCTGTGC CTAAGCATCC GTATGCTGCT ATGGAGAACT GGGGACTAAG TATTTTTGTG 
GAACAAAGAA TACTGCTGGA TCCCAGTGTT TCATCTATTT CTTATTTGCT GGATGTCACC 
ATGGTCATTG TTCATGAGAT ATGTCACCAG TGGTTTGGTG ACCTTGTGAC GCCTGTGTGG 
TGGGAAGACG TGTGGCTGAA GGAAGGGTTT GCTCACTACT TTGAATTTGT TGGTACAGAC 
TACCTCTATC CTGGCTGGAA CATGGAAAAG CAGAGGTTTC TGACCGATGT TCTGCATGAA 
GTGATGCTGC TGGACGGTTT GGCCAGTTCC CATCCAGTAT CACAGGAAGT GCTGCAGGCA 
ACAGATATTG ACAGGGTGTT TGACTGGATC GCATATAAAA AGGGTGCTGC TTTAATAAGA 
ATGCTGGCTA ATTTTATGGG CCATTCAGTT TTCCAGAGGG GTTTGCAAGA TTATTTAACC 
ATTCATAAGT ATGGTAATGC AGCCAGAAAT GATCTCTGGA ATACATTATC GGAGGCTTTA 
AAAAGAAATG GGAAATATGT AAATATACAA GAAGTAATGG ATCAGTGGAC ACTCCAGATG 
GGTTATCCTG TTATCACCAT CTTGGGAAAC ACAACAGCAG AAAATAGAAT AATAATTACC 
CAACAGCATT TTATCTATGA TATCAGTGCT AAAACTAAAG CACTTAAACT TCAGAATAAC 
AGTTACCTGT GGCAGATTCC ATTAACTATT GTGGTAGGAA ATAGAAGCCA TGTGTCTTCA 
GAAGCAATTA TTTGGGTGTC TAACAAATCA GAGCACCACA GAATAACTTA TTTGGACAAA 
GGAAGCTGGC TGCTGGGGAA CATCAATCAA ACTGGCTATT TTAGAGTCAA CTATGACCTA 
AGGAACTGGA GATTATTAAT TGATCAATTA ATCCGGAATC ATGAGGTTCT TTCTGTCAGT 
AACCGAGCGG GCTTGATCGA TGATGCCTTC AGCCTAGCCA GGGCTGGCTA TTTGCCTCAG 
AATATTCCTC TGGAGATTAT CAGATACCTG TCTGAGGAGA AGGATTTTCT TCCTTGGCAT 
GCTGCCAGCC GAGCTCTTTA TCCTCTAGAT AAATTACTGG ACCGCATGGA AAACTACAAC 
ATTTTCAATG AATATATTTT AAAGCAAGTT GCAACAACAT ATATCAAGCT TGGGTGGCCG 
AAAAATAATT TTAATGGATC TCTTGTTCAA GCATCCTACC AACATGAAGA ACTACGTAGA 
GAAGTTATAA TGCTGGCCTG CAGTTTTGGC AACAAGCACT GTCACCAACA GGCATCAACA 
CTTATTTCAG ATTGGATTTC CAGCAACAGG AACAGAATAC CACTAAATGT TAGAGACATC 
GTATACTGTA CAGGAGTGTC ACTACTGGAT GAGGATGTCT GGGAATTCAT ATGGATGAAA 
TTCCATTCCA CCACAGCAGT TTCTGAGAAG AAAATATTAT TGGAAGCCTT AACTTGCAGT 
GATGACAGGA ATTTATTAAA CAGGCTTCTA AATCTGTCAC TGAATTCTGA GGTGGTGCTG 
GATCAAGATG CAATTGATGT CATAATCCAT GTAGCTCGAA ATCCACATGG TCGAGACCTT 
GCCTGGAAGT TTTTCAGGGA TAAATGGAAG ATATTAAATA CCAGTTGCAG TAAAATTTAC 
TCCCAGGACA TCTTGTTTCA TGGTGAGAAT TCTTTAGCCT CAATTTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874732.1
CDS	1..3051
Translation	MALDGELGEQEEEKKKKKKKKRKKKKEEEEEEEGAEKSSSPFAAAMGEDDAALRAGSRGLSDPWADSVGVRPRTTERHIAVHKRLVLAFAVSLVALLAVTMLAVLLSLRFDECGASATPGADGGPSGFPERGGNGSLPGSARRNHHAGGDSWQPEAGGVASPGTTSAQPPSEEEREPWEPWTQLRLSGHLKPLHYNLMLTAFMENFTFSGEVNVEIACRNATRYVVLHASRVAVEKVQLAEDRAFGAVPVAGFFLYPQTQVLVVVLNRTLDAQRNYNLKIIYNALIENELLGFFRSSYVLHGERRFLGVTQFSPTHARKAFPCFDEPIYKATFKISIKHQATYLSLSNMPVETSVFEEDGWVTDHFSQTPLMSTYYLAWAICNFTYRETTTKSGVVVRLYARPDAIRRGSGDYALHITKRLIEFYEDYFKVPYSLPKLDLLAVPKHPYAAMENWGLSIFVEQRILLDPSVSSISYLLDVTMVIVHEICHQWFGDLVTPVWWEDVWLKEGFAHYFEFVGTDYLYPGWNMEKQRFLTDVLHEVMLLDGLASSHPVSQEVLQATDIDRVFDWIAYKKGAALIRMLANFMGHSVFQRGLQDYLTIHKYGNAARNDLWNTLSEALKRNGKYVNIQEVMDQWTLQMGYPVITILGNTTAENRIIITQQHFIYDISAKTKALKLQNNSYLWQIPLTIVVGNRSHVSSEAIIWVSNKSEHHRITYLDKGSWLLGNINQTGYFRVNYDLRNWRLLIDQLIRNHEVLSVSNRAGLIDDAFSLARAGYLPQNIPLEIIRYLSEEKDFLPWHAASRALYPLDKLLDRMENYNIFNEYILKQVATTYIKLGWPKNNFNGSLVQASYQHEELRREVIMLACSFGNKHCHQQASTLISDWISSNRNRIPLNVRDIVYCTGVSLLDEDVWEFIWMKFHSTTAVSEKKILLEALTCSDDRNLLNRLLNLSLNSEVVLDQDAIDVIIHVARNPHGRDLAWKFFRDKWKILNTSCSKIYSQDILFHGENSLASIL

Target ORF information:

RefSeq Version	XM_017019243.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyrotropin releasing hormone degrading enzyme (TRHDE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019243.1

ORF Insert Sequence:

ATGGCCCTGG ACGGCGAGCT GGGGGAGCAA GAGGAGGAGA AGAAAAAGAA GAAGAAAAAG 
AAGAGGAAGA AGAAGAAGGA GGAGGAGGAG GAGGAGGAGG GGGCCGAGAA GAGCAGCTCA 
CCCTTCGCAG CCGCGATGGG GGAAGACGAC GCCGCGCTTC GGGCTGGCAG CAGGGGGCTC 
TCCGACCCGT GGGCAGACTC AGTGGGAGTG CGACCCCGCA CCACGGAGCG CCACATCGCC 
GTACACAAGC GGCTTGTGCT GGCCTTCGCT GTGTCCCTCG TGGCATTGCT CGCGGTCACA 
ATGCTCGCTG TGCTGCTCAG CCTGCGCTTC GACGAGTGCG GGGCGAGTGC CACGCCAGGC 
GCCGACGGTG GCCCCTCAGG CTTTCCGGAG CGCGGCGGCA ACGGGAGCCT CCCTGGATCG 
GCCCGGCGCA ACCACCACGC AGGCGGGGAC TCCTGGCAGC CCGAGGCGGG TGGGGTGGCC 
AGTCCGGGGA CCACGTCGGC CCAGCCGCCG TCGGAGGAGG AGCGGGAGCC GTGGGAGCCG 
TGGACGCAGC TGCGCCTGTC GGGCCACCTG AAGCCGCTGC ACTACAATCT GATGCTCACC 
GCCTTCATGG AGAACTTCAC CTTCTCCGGG GAGGTCAACG TGGAGATCGC GTGCCGGAAC 
GCCACCCGCT ACGTAGTGCT GCACGCTTCC CGAGTGGCGG TGGAGAAAGT GCAGCTGGCC 
GAGGACCGGG CGTTCGGGGC TGTCCCTGTA GCCGGTTTTT TCCTCTACCC GCAAACCCAG 
GTCTTAGTGG TGGTGCTGAA TAGGACACTG GACGCGCAGA GGAATTACAA TCTGAAGATT 
ATCTACAACG CGCTCATCGA GAATGAGCTC CTGGGCTTCT TCCGCAGCTC CTATGTGCTC 
CACGGGGAGA GAAGATTCCT TGGTGTTACT CAGTTTTCGC CTACACATGC CAGAAAGGCA 
TTTCCTTGTT TTGATGAGCC AATCTACAAG GCTACTTTCA AAATCAGCAT CAAGCATCAA 
GCAACCTATT TATCTTTATC TAATATGCCA GTGGAAACTT CCGTGTTTGA GGAAGATGGA 
TGGGTTACGG ATCACTTTTC ACAGACCCCT CTCATGTCCA CATATTATTT AGCCTGGGCA 
ATTTGCAACT TCACATACAG AGAAACTACC ACCAAGAGTG GGGTTGTAGT ACGATTATAT 
GCAAGACCTG ATGCTATCAG AAGAGGATCC GGGGACTATG CTCTCCATAT AACAAAGAGA 
TTAATAGAAT TTTATGAAGA CTACTTTAAA GTGCCCTATT CCTTGCCAAA ACTAGATCTT 
TTAGCTGTGC CTAAGCATCC GTATGCTGCT ATGGAGAACT GGGGACTAAG TATTTTTGTG 
GAACAAAGAA TACTGCTGGA TCCCAGTGTT TCATCTATTT CTTATTTGCT GGATGTCACC 
ATGGTCATTG TTCATGAGAT ATGTCACCAG TGGTTTGGTG ACCTTGTGAC GCCTGTGTGG 
TGGGAAGACG TGTGGCTGAA GGAAGGGTTT GCTCACTACT TTGAATTTGT TGGTACAGAC 
TACCTCTATC CTGGCTGGAA CATGGAAAAG CAGAGGTTTC TGACCGATGT TCTGCATGAA 
GTGATGCTGC TGGACGGTTT GGCCAGTTCC CATCCAGTAT CACAGGAAGT GCTGCAGGCA 
ACAGATATTG ACAGGGTGTT TGACTGGATC GCATATAAAA AGGGTGCTGC TTTAATAAGA 
ATGCTGGCTA ATTTTATGGG CCATTCAGTT TTCCAGAGGG GTTTGCAAGA TTATTTAACC 
ATTCATAAGT ATGGTAATGC AGCCAGAAAT GATCTCTGGA ATACATTATC GGAGGCTTTA 
AAAAGAAATG GGAAATATGT AAATATACAA GAAGTAATGG ATCAGTGGAC ACTCCAGATG 
GGTTATCCTG TTATCACCAT CTTGGGAAAC ACAACAGCAG AAAATAGAAT AATAATTACC 
CAACAGCATT TTATCTATGA TATCAGTGCT AAAACTAAAG CACTTAAACT TCAGAATAAC 
AGTTACCTGT GGCAGATTCC ATTAACTATT GTGGTAGGAA ATAGAAGCCA TGTGTCTTCA 
GAAGCAATTA TTTGGGTGTC TAACAAATCA GAGCACCACA GAATAACTTA TTTGGACAAA 
GGAAGCTGGC TGCTGGGGAA CATCAATCAA ACTGGCTATT TTAGAGTCAA CTATGACCTA 
AGGAACTGGA GATTATTAAT TGATCAATTA ATCCGGAATC ATGAGGTTCT TTCTGTCAGT 
AACCGAGCGG GCTTGATCGA TGATGCCTTC AGCCTAGCCA GGGCTGGCTA TTTGCCTCAG 
AATATTCCTC TGGAGATTAT CAGATACCTG TCTGAGGAGA AGGATTTTCT TCCTTGGCAT 
GCTGCCAGCC GAGCTCTTTA TCCTCTAGAT AAATTACTGG ACCGCATGGA AAACTACAAC 
ATTTTCAATG AATATATTTT AAAGCAAGTT GCAACAACAT ATATCAAGCT TGGGTGGCCG 
AAAAATAATT TTAATGGATC TCTTGTTCAA GCATCCTACC AACATGAAGA ACTACGTAGA 
GAAGTTATAA TGCTGGCCTG CAGTTTTGGC AACAAGCACT GTCACCAACA GGCATCAACA 
CTTATTTCAG ATTGGATTTC CAGCAACAGG AACAGAATAC CACTAAATGT TAGAGACATC 
GTATACTGTA CAGGAGTGTC ACTACTGGAT GAGGATGTCT GGGAATTCAT ATGGATGAAA 
TTCCATTCCA CCACAGCAGT TTCTGAGAAG AAAATATTAT TGGAAGCCTT AACTTGCAGT 
GATGACAGGA ATTTATTAAA CAGGCTTCTA AATCTGTCAC TGAATTCTGA GGTGGTGCTG 
GATCAAGATG CAATTGATGT CATAATCCAT GTAGCTCGAA ATCCACATGG TCGAGACCTT 
GCCTGGAAGT TTTTCAGGGA TAAATGGAAG ATATTAAATA CCAGTTGCAG TAAAATTTAC 
TCCCAGGACA TCTTGTTTCA TGGTGAGAAT TCTTTAGCCT CAATTTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide association study of a heart failure related metabolomic profile among African Americans in the Atherosclerosis Risk in Communities (ARIC) study.
Genetic epidemiology37(8)840-5(2013 Dec)
Yu B,Zheng Y,Alexander D,Manolio TA,Alonso A,Nettleton JA,Boerwinkle E

Genome-wide association study of rheumatoid arthritis in Koreans: population-specific loci as well as overlap with European susceptibility loci.
Arthritis and rheumatism63(4)884-93(2011 Apr)
Freudenberg J,Lee HS,Han BG,Shin HD,Kang YM,Sung YK,Shim SC,Choi CB,Lee AT,Gregersen PK,Bae SC

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Diabetes care33(10)2250-3(2010 Oct)
Bailey SD,Xie C,Do R,Montpetit A,Diaz R,Mohan V,Keavney B,Yusuf S,Gerstein HC,Engert JC,Anand S,

Chemerin, a novel adipokine in the regulation of angiogenesis.
The Journal of clinical endocrinology and metabolism95(5)2476-85(2010 May)
Bozaoglu K,Curran JE,Stocker CJ,Zaibi MS,Segal D,Konstantopoulos N,Morrison S,Carless M,Dyer TD,Cole SA,Goring HH,Moses EK,Walder K,Cawthorne MA,Blangero J,Jowett JB

Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.
American journal of human genetics85(5)628-42(2009 Nov)
Talmud PJ,Drenos F,Shah S,Shah T,Palmen J,Verzilli C,Gaunt TR,Pallas J,Lovering R,Li K,Casas JP,Sofat R,Kumari M,Rodriguez S,Johnson T,Newhouse SJ,Dominiczak A,Samani NJ,Caulfield M,Sever P,Stanton A,Shields DC,Padmanabhan S,Melander O,Hastie C,Delles C,Ebrahim S,Marmot MG,Smith GD,Lawlor DA,Munroe PB,Day IN,Kivimaki M,Whittaker J,Humphries SE,Hingorani AD,,,

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TRHDE cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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