NENF cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NENF
Entrez Gene ID	29937
Full Name	neudesin neurotrophic factor
Synonyms	CIR2,SCIRP10,SPUF
General protein information	Preferred Names neudesin neurotrophic factor Names neudesin SCIRP10-related protein Spinal cord injury related protein 10 cell growth-inhibiting protein 47 cell immortalization-related protein 2 neuron-derived neurotrophic factor secreted protein of unknown function
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q32.3
Summary	This gene encodes a neurotrophic factor that may play a role in neuron differentiation and development. A pseudogene of this gene is found on chromosome 12. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2009].
Disorder MIM:	611874

mRNA and Protein(s)

mRNA	Protein	Name
NM_013349.5	NP_037481.1	neudesin precursor
NM_013349.4	NP_037481.1	neudesin precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Nenf	NP_079700.1
Danio rerio (zebrafish)	nenf	NP_001032793.2
Pan troglodytes (chimpanzee)	NENF	XP_514182.2
Rattus norvegicus (Norway rat)	Nenf	NP_001002851.1
Arabidopsis thaliana (thale cress)	MAPR2	NP_180066.1
Gallus gallus (chicken)	NENF	XP_419430.1
Xenopus tropicalis (tropical clawed frog)	nenf	NP_001025625.1
Homo sapiens (human)	NENF	NP_037481.1
	LOC710911	XP_001108201.1
Bos taurus (cattle)	NENF	NP_001069887.1
Oryza sativa (rice)	Os02g0793700	NP_001048371.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide study of hypomethylated and induced genes in patients with liver cancer unravels novel anticancer targets.
Stefanska B, Cheishvili D, Suderman M, Arakelian A, Huang J, Hallett M, Han ZG, Al-Mahtab M, Akbar SM, Khan WA, Raqib R, Tanvir I, Khan HA, Rabbani SA, Szyf M
Clinical cancer research : an official journal of the American Association for Cancer Research20(12)3118-32(2014 Jun)

Neurotrophic activity of neudesin, a novel extracellular heme-binding protein, is dependent on the binding of heme to its cytochrome b5-like heme/steroid-binding domain.
Kimura I, Nakayama Y, Yamauchi H, Konishi M, Miyake A, Mori M, Ohta M, Itoh N, Fujimoto M
The Journal of biological chemistry283(7)4323-31(2008 Feb)

Neudesin, a novel secreted protein with a unique primary structure and neurotrophic activity.
Kimura I, Yoshioka M, Konishi M, Miyake A, Itoh N
Journal of neuroscience research79(3)287-94(2005 Feb)

Up-regulation of CIR1/CROC1 expression upon cell immortalization and in tumor-derived human cell lines.
Ma L, Broomfield S, Lavery C, Lin SL, Xiao W, Bacchetti S
Oncogene17(10)1321-6(1998 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High NENF expression is associated with liver cancer.
Title: Genome-wide study of hypomethylated and induced genes in patients with liver cancer unravels novel anticancer targets.

Describes the predicted heme-binding domains in the human neudesin protein sequence.
Title: Neurotrophic activity of neudesin, a novel extracellular heme-binding protein, is dependent on the binding of heme to its cytochrome b5-like heme/steroid-binding domain.

Human neudesin of 172 amino acids with high similarity ( approximately 91% identity) to mouse neudesin was identified; the human neudesin gene was mapped to chromosome 1p33.
Title: Neudesin, a novel secreted protein with a unique primary structure and neurotrophic activity.

The following NENF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NENF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31427	NM_013349.5 Latest version!	Homo sapiens neudesin neurotrophic factor (NENF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
View Old Accession Versions >>
OHu31427	NM_013349.4	Homo sapiens neudesin neurotrophic factor (NENF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NENF ( NM_013349.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_037481.1 Homo sapiens neudesin neurotrophic factor (NENF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31427
Clone ID Related Accession (Same CDS sequence)	NM_013349.5 , NM_013349.4
Accession Version	NM_013349.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	neudesin precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF173937.1, DB574171.1 and BC008823.2. On Nov 22, 2018 this sequence version replaced NM_013349.4. Transcript Variant: This variant (1) represents the longer transcript. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.296030.1, SRR1163658.413969.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000366988.5/ ENSP00000355955.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGGGCC CCGCGCCGCG GCGGCGGCTG CGGCCGCTGG CAGCGCTGGC CCTGGTCCTG 
GCGCTGGCCC CGGGGCTGCC CACAGCCCGG GCCGGGCAGA CACCGCGCCC TGCCGAGCGG 
GGGCCCCCAG TGCGGCTTTT CACCGAGGAG GAGCTGGCCC GCTATGGCGG GGAGGAGGAA 
GATCAGCCCA TCTACTTGGC AGTGAAGGGA GTGGTGTTTG ATGTCACCTC CGGAAAGGAG 
TTTTATGGAC GAGGAGCCCC CTACAATGCC TTGACGGGGA AGGACTCCAC TAGAGGGGTA 
GCCAAGATGT CCTTGGATCC TGCAGACCTC ACCCATGACA CTACGGGTCT CACGGCCAAG 
GAACTGGAGG CCCTGGATGA GGTCTTCACC AAAGTGTACA AAGCCAAATA CCCCATCGTC 
GGCTACACTG CCCGGAGAAT TCTCAATGAG GATGGCAGCC CTAACCTGGA CTTCAAGCCT 
GAAGACCAGC CCCATTTTGA CATCAAGGAT GAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037481.1
CDS	25..543
Translation	MVGPAPRRRLRPLAALALVLALAPGLPTARAGQTPRPAERGPPVRLFTEEELARYGGEEEDQPIYLAVKGVVFDVTSGKEFYGRGAPYNALTGKDSTRGVAKMSLDPADLTHDTTGLTAKELEALDEVFTKVYKAKYPIVGYTARRILNEDGSPNLDFKPEDQPHFDIKDEF

Target ORF information:

RefSeq Version	NM_013349.5
Organism	Homo sapiens(human)
Definition	Homo sapiens neudesin neurotrophic factor (NENF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013349.5

ORF Insert Sequence:

ATGGTGGGCC CCGCGCCGCG GCGGCGGCTG CGGCCGCTGG CAGCGCTGGC CCTGGTCCTG 
GCGCTGGCCC CGGGGCTGCC CACAGCCCGG GCCGGGCAGA CACCGCGCCC TGCCGAGCGG 
GGGCCCCCAG TGCGGCTTTT CACCGAGGAG GAGCTGGCCC GCTATGGCGG GGAGGAGGAA 
GATCAGCCCA TCTACTTGGC AGTGAAGGGA GTGGTGTTTG ATGTCACCTC CGGAAAGGAG 
TTTTATGGAC GAGGAGCCCC CTACAATGCC TTGACGGGGA AGGACTCCAC TAGAGGGGTA 
GCCAAGATGT CCTTGGATCC TGCAGACCTC ACCCATGACA CTACGGGTCT CACGGCCAAG 
GAACTGGAGG CCCTGGATGA GGTCTTCACC AAAGTGTACA AAGCCAAATA CCCCATCGTC 
GGCTACACTG CCCGGAGAAT TCTCAATGAG GATGGCAGCC CTAACCTGGA CTTCAAGCCT 
GAAGACCAGC CCCATTTTGA CATCAAGGAT GAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NENF ( NM_013349.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_037481.1 Homo sapiens neudesin neurotrophic factor (NENF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31427
Clone ID Related Accession (Same CDS sequence)	NM_013349.5 , NM_013349.4
Accession Version	NM_013349.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	neudesin precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF173937.1, DB574171.1 and BC008823.2. On Jan 24, 2009 this sequence version replaced NM_013349.3. Transcript Variant: This variant (1) represents the longer transcript. ##Evidence-Data-START## Transcript exon combination :: BC008823.2, SRR1163658.413969.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGGGCC CCGCGCCGCG GCGGCGGCTG CGGCCGCTGG CAGCGCTGGC CCTGGTCCTG 
GCGCTGGCCC CGGGGCTGCC CACAGCCCGG GCCGGGCAGA CACCGCGCCC TGCCGAGCGG 
GGGCCCCCAG TGCGGCTTTT CACCGAGGAG GAGCTGGCCC GCTATGGCGG GGAGGAGGAA 
GATCAGCCCA TCTACTTGGC AGTGAAGGGA GTGGTGTTTG ATGTCACCTC CGGAAAGGAG 
TTTTATGGAC GAGGAGCCCC CTACAATGCC TTGACGGGGA AGGACTCCAC TAGAGGGGTA 
GCCAAGATGT CCTTGGATCC TGCAGACCTC ACCCATGACA CTACGGGTCT CACGGCCAAG 
GAACTGGAGG CCCTGGATGA GGTCTTCACC AAAGTGTACA AAGCCAAATA CCCCATCGTC 
GGCTACACTG CCCGGAGAAT TCTCAATGAG GATGGCAGCC CTAACCTGGA CTTCAAGCCT 
GAAGACCAGC CCCATTTTGA CATCAAGGAT GAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037481.1
CDS	58..576
Translation	MVGPAPRRRLRPLAALALVLALAPGLPTARAGQTPRPAERGPPVRLFTEEELARYGGEEEDQPIYLAVKGVVFDVTSGKEFYGRGAPYNALTGKDSTRGVAKMSLDPADLTHDTTGLTAKELEALDEVFTKVYKAKYPIVGYTARRILNEDGSPNLDFKPEDQPHFDIKDEF

Target ORF information:

RefSeq Version	NM_013349.4
Organism	Homo sapiens(human)
Definition	Homo sapiens neudesin neurotrophic factor (NENF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013349.4

ORF Insert Sequence:

ATGGTGGGCC CCGCGCCGCG GCGGCGGCTG CGGCCGCTGG CAGCGCTGGC CCTGGTCCTG 
GCGCTGGCCC CGGGGCTGCC CACAGCCCGG GCCGGGCAGA CACCGCGCCC TGCCGAGCGG 
GGGCCCCCAG TGCGGCTTTT CACCGAGGAG GAGCTGGCCC GCTATGGCGG GGAGGAGGAA 
GATCAGCCCA TCTACTTGGC AGTGAAGGGA GTGGTGTTTG ATGTCACCTC CGGAAAGGAG 
TTTTATGGAC GAGGAGCCCC CTACAATGCC TTGACGGGGA AGGACTCCAC TAGAGGGGTA 
GCCAAGATGT CCTTGGATCC TGCAGACCTC ACCCATGACA CTACGGGTCT CACGGCCAAG 
GAACTGGAGG CCCTGGATGA GGTCTTCACC AAAGTGTACA AAGCCAAATA CCCCATCGTC 
GGCTACACTG CCCGGAGAAT TCTCAATGAG GATGGCAGCC CTAACCTGGA CTTCAAGCCT 
GAAGACCAGC CCCATTTTGA CATCAAGGAT GAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide study of hypomethylated and induced genes in patients with liver cancer unravels novel anticancer targets.
Clinical cancer research : an official journal of the American Association for Cancer Research20(12)3118-32(2014 Jun)
Stefanska B,Cheishvili D,Suderman M,Arakelian A,Huang J,Hallett M,Han ZG,Al-Mahtab M,Akbar SM,Khan WA,Raqib R,Tanvir I,Khan HA,Rabbani SA,Szyf M

Neurotrophic activity of neudesin, a novel extracellular heme-binding protein, is dependent on the binding of heme to its cytochrome b5-like heme/steroid-binding domain.
The Journal of biological chemistry283(7)4323-31(2008 Feb)
Kimura I,Nakayama Y,Yamauchi H,Konishi M,Miyake A,Mori M,Ohta M,Itoh N,Fujimoto M

Neudesin, a novel secreted protein with a unique primary structure and neurotrophic activity.
Journal of neuroscience research79(3)287-94(2005 Feb)
Kimura I,Yoshioka M,Konishi M,Miyake A,Itoh N

Up-regulation of CIR1/CROC1 expression upon cell immortalization and in tumor-derived human cell lines.
Oncogene17(10)1321-6(1998 Sep)
Ma L,Broomfield S,Lavery C,Lin SL,Xiao W,Bacchetti S