Nenf cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nenf
Entrez Gene ID	66208
Full Name	neuron derived neurotrophic factor
Synonyms	1110060M21Rik,SCIRP10,Spuf
General protein information	Preferred Names neuron derived neurotrophic factor Names neudesin SCIRP10-related protein Spinal cord injury related protein 10 secreted protein of unknown function
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 H6

mRNA and Protein(s)

mRNA	Protein	Name
NM_025424.3	NP_079700.1	neudesin precursor
NM_025424.2	NP_079700.1	neudesin precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Nenf	NP_079700.1
Danio rerio (zebrafish)	nenf	NP_001032793.2
Pan troglodytes (chimpanzee)	NENF	XP_514182.2
Rattus norvegicus (Norway rat)	Nenf	NP_001002851.1
Arabidopsis thaliana (thale cress)	MAPR2	NP_180066.1
Gallus gallus (chicken)	NENF	XP_419430.1
Xenopus tropicalis (tropical clawed frog)	nenf	NP_001025625.1
Homo sapiens (human)	NENF	NP_037481.1
	LOC710911	XP_001108201.1
Bos taurus (cattle)	NENF	NP_001069887.1
Oryza sativa (rice)	Os02g0793700	NP_001048371.1

Gene Ontology
Bibliography

Related articles in PubMed

Deletion of the Neurotrophic Factor neudesin Prevents Diet-induced Obesity by Increased Sympathetic Activity.
Ohta H, Konishi M, Kobayashi Y, Kashio A, Mochiyama T, Matsumura S, Inoue K, Fushiki T, Nakao K, Kimura I, Itoh N
Scientific reports510049(2015 May)

Identification of hypothalamic neuron-derived neurotrophic factor as a novel factor modulating appetite.
Byerly MS, Swanson RD, Semsarzadeh NN, McCulloh PS, Kwon K, Aja S, Moran TH, Wong GW, Blackshaw S
American journal of physiology. Regulatory, integrative and comparative physiology304(12)R1085-95(2013 Jun)

Neudesin is involved in anxiety behavior: structural and neurochemical correlates.
Novais A, Ferreira AC, Marques F, P?go JM, Cerqueira JJ, David-Pereira A, Campos FL, Dalla C, Kokras N, Sousa N, Palha JA, Sousa JC
Frontiers in behavioral neuroscience7119(2013)

Neudesin, an extracellular heme-binding protein, suppresses adipogenesis in 3T3-L1 cells via the MAPK cascade.
Kimura I, Konishi M, Asaki T, Furukawa N, Ukai K, Mori M, Hirasawa A, Tsujimoto G, Ohta M, Itoh N, Fujimoto M
Biochemical and biophysical research communications381(1)75-80(2009 Mar)

Neurotrophic activity of neudesin, a novel extracellular heme-binding protein, is dependent on the binding of heme to its cytochrome b5-like heme/steroid-binding domain.
Kimura I, Nakayama Y, Yamauchi H, Konishi M, Miyake A, Mori M, Ohta M, Itoh N, Fujimoto M
The Journal of biological chemistry283(7)4323-31(2008 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

neudesin, which may be a negative regulator of sympathetic activity, could represent a novel regulator of the development of obesity and obesity-related metabolic dysfunctions.
Title: Deletion of the Neurotrophic Factor neudesin Prevents Diet-induced Obesity by Increased Sympathetic Activity.

Neuron-derived neurotrophic factor (NENF) is an important central modulator of food intake.
Title: Identification of hypothalamic neuron-derived neurotrophic factor as a novel factor modulating appetite.

These findings suggest that neudesin plays a critical role in the early stage of adipocyte differentiation in which C/EBPbeta induces PPARgamma and C/EBPalpha expressions, by controlling the MAPK pathway.
Title: Neudesin, an extracellular heme-binding protein, suppresses adipogenesis in 3T3-L1 cells via the MAPK cascade.

Neudesin is the first extracellular heme-binding protein that shows signal transducing activity by itself. The present findings may shed new light on the function of extracellular heme-binding proteins
Title: Neurotrophic activity of neudesin, a novel extracellular heme-binding protein, is dependent on the binding of heme to its cytochrome b5-like heme/steroid-binding domain.

The present findings have revealed new potential roles of neudesin in neural cell proliferation and neuronal differentiation.
Title: Neudesin, a secreted factor, promotes neural cell proliferation and neuronal differentiation in mouse neural precursor cells.

The following Nenf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nenf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19208	NM_025424.3 Latest version!	Mus musculus neuron derived neurotrophic factor (Nenf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OMu19208	NM_025424.2	Mus musculus neuron derived neurotrophic factor (Nenf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nenf ( NM_025424.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079700.1 Mus musculus neuron derived neurotrophic factor (Nenf), mRNA.

Price & Availability↑

CloneID	OMu19208
Clone ID Related Accession (Same CDS sequence)	NM_025424.3 , NM_025424.2
Accession Version	NM_025424.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	neudesin precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115896.9 and AK002413.1. On Jan 31, 2019 this sequence version replaced NM_025424.2. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.2497068.1, SRR7345562.4916403.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCGCC CCGCGCCCTG GTGGCGGCTG CGGCTGCTGG CGGCGCTCGT CCTGGCGCTG 
GCTCTGGTCC CAGTGCCCTC AGCCTGGGCT GGGCAGACGC CGCGCCCCGC AGAGCGCGGG 
CCCCCGGTGC GGCTCTTCAC CGAGGAGGAG CTGGCCCGCT ACGGCGGCGA GGAGGAGGAT 
CAGCCCATCT ACTTGGCAGT GAAGGGAGTG GTGTTCGATG TCACCTCTGG GAAGGAGTTT 
TATGGACGAG GAGCCCCCTA CAATGCCTTG GCCGGGAAGG ACTCCAGCAG AGGTGTGGCC 
AAGATGTCAC TGGATCCAGC AGACCTCACT CACGACACTA CTGGTCTCAC GGCCAAGGAG 
CTGGAGGCCC TCGATGACGT CTTCAGCAAG GTGTACAAAG CCAAGTACCC CATTGTTGGC 
TACACAGCCC GAAGGATCCT CAACGAGGAT GGCAGCCCCA ACCTGGACTT CAAGCCTGAA 
GACCAGCCCC ATTTTGACAT AAAGGACGAA TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079700.1
CDS	40..555
Translation	MARPAPWWRLRLLAALVLALALVPVPSAWAGQTPRPAERGPPVRLFTEEELARYGGEEEDQPIYLAVKGVVFDVTSGKEFYGRGAPYNALAGKDSSRGVAKMSLDPADLTHDTTGLTAKELEALDDVFSKVYKAKYPIVGYTARRILNEDGSPNLDFKPEDQPHFDIKDEF

Target ORF information:

RefSeq Version	NM_025424.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuron derived neurotrophic factor (Nenf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025424.3

ORF Insert Sequence:

ATGGCGCGCC CCGCGCCCTG GTGGCGGCTG CGGCTGCTGG CGGCGCTCGT CCTGGCGCTG 
GCTCTGGTCC CAGTGCCCTC AGCCTGGGCT GGGCAGACGC CGCGCCCCGC AGAGCGCGGG 
CCCCCGGTGC GGCTCTTCAC CGAGGAGGAG CTGGCCCGCT ACGGCGGCGA GGAGGAGGAT 
CAGCCCATCT ACTTGGCAGT GAAGGGAGTG GTGTTCGATG TCACCTCTGG GAAGGAGTTT 
TATGGACGAG GAGCCCCCTA CAATGCCTTG GCCGGGAAGG ACTCCAGCAG AGGTGTGGCC 
AAGATGTCAC TGGATCCAGC AGACCTCACT CACGACACTA CTGGTCTCAC GGCCAAGGAG 
CTGGAGGCCC TCGATGACGT CTTCAGCAAG GTGTACAAAG CCAAGTACCC CATTGTTGGC 
TACACAGCCC GAAGGATCCT CAACGAGGAT GGCAGCCCCA ACCTGGACTT CAAGCCTGAA 
GACCAGCCCC ATTTTGACAT AAAGGACGAA TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nenf ( NM_025424.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079700.1 Mus musculus neuron derived neurotrophic factor (Nenf), mRNA.

Price & Availability↑

CloneID	OMu19208
Clone ID Related Accession (Same CDS sequence)	NM_025424.3 , NM_025424.2
Accession Version	NM_025424.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-10
Organism	Mus musculus(house mouse)
Product	neudesin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK002413.1. On Apr 6, 2007 this sequence version replaced NM_025424.1. ##Evidence-Data-START## Transcript exon combination :: AK002413.1, AK155319.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCGCC CCGCGCCCTG GTGGCGGCTG CGGCTGCTGG CGGCGCTCGT CCTGGCGCTG 
GCTCTGGTCC CAGTGCCCTC AGCCTGGGCT GGGCAGACGC CGCGCCCCGC AGAGCGCGGG 
CCCCCGGTGC GGCTCTTCAC CGAGGAGGAG CTGGCCCGCT ACGGCGGCGA GGAGGAGGAT 
CAGCCCATCT ACTTGGCAGT GAAGGGAGTG GTGTTCGATG TCACCTCTGG GAAGGAGTTT 
TATGGACGAG GAGCCCCCTA CAATGCCTTG GCCGGGAAGG ACTCCAGCAG AGGTGTGGCC 
AAGATGTCAC TGGATCCAGC AGACCTCACT CACGACACTA CTGGTCTCAC GGCCAAGGAG 
CTGGAGGCCC TCGATGACGT CTTCAGCAAG GTGTACAAAG CCAAGTACCC CATTGTTGGC 
TACACAGCCC GAAGGATCCT CAACGAGGAT GGCAGCCCCA ACCTGGACTT CAAGCCTGAA 
GACCAGCCCC ATTTTGACAT AAAGGACGAA TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079700.1
CDS	27..542
Translation	MARPAPWWRLRLLAALVLALALVPVPSAWAGQTPRPAERGPPVRLFTEEELARYGGEEEDQPIYLAVKGVVFDVTSGKEFYGRGAPYNALAGKDSSRGVAKMSLDPADLTHDTTGLTAKELEALDDVFSKVYKAKYPIVGYTARRILNEDGSPNLDFKPEDQPHFDIKDEF

Target ORF information:

RefSeq Version	NM_025424.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuron derived neurotrophic factor (Nenf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025424.2

ORF Insert Sequence:

ATGGCGCGCC CCGCGCCCTG GTGGCGGCTG CGGCTGCTGG CGGCGCTCGT CCTGGCGCTG 
GCTCTGGTCC CAGTGCCCTC AGCCTGGGCT GGGCAGACGC CGCGCCCCGC AGAGCGCGGG 
CCCCCGGTGC GGCTCTTCAC CGAGGAGGAG CTGGCCCGCT ACGGCGGCGA GGAGGAGGAT 
CAGCCCATCT ACTTGGCAGT GAAGGGAGTG GTGTTCGATG TCACCTCTGG GAAGGAGTTT 
TATGGACGAG GAGCCCCCTA CAATGCCTTG GCCGGGAAGG ACTCCAGCAG AGGTGTGGCC 
AAGATGTCAC TGGATCCAGC AGACCTCACT CACGACACTA CTGGTCTCAC GGCCAAGGAG 
CTGGAGGCCC TCGATGACGT CTTCAGCAAG GTGTACAAAG CCAAGTACCC CATTGTTGGC 
TACACAGCCC GAAGGATCCT CAACGAGGAT GGCAGCCCCA ACCTGGACTT CAAGCCTGAA 
GACCAGCCCC ATTTTGACAT AAAGGACGAA TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deletion of the Neurotrophic Factor neudesin Prevents Diet-induced Obesity by Increased Sympathetic Activity.
Scientific reports510049(2015 May)
Ohta H,Konishi M,Kobayashi Y,Kashio A,Mochiyama T,Matsumura S,Inoue K,Fushiki T,Nakao K,Kimura I,Itoh N

Identification of hypothalamic neuron-derived neurotrophic factor as a novel factor modulating appetite.
American journal of physiology. Regulatory, integrative and comparative physiology304(12)R1085-95(2013 Jun)
Byerly MS,Swanson RD,Semsarzadeh NN,McCulloh PS,Kwon K,Aja S,Moran TH,Wong GW,Blackshaw S

Neudesin is involved in anxiety behavior: structural and neurochemical correlates.
Frontiers in behavioral neuroscience7119(2013)
Novais A,Ferreira AC,Marques F,P?go JM,Cerqueira JJ,David-Pereira A,Campos FL,Dalla C,Kokras N,Sousa N,Palha JA,Sousa JC

Neudesin, an extracellular heme-binding protein, suppresses adipogenesis in 3T3-L1 cells via the MAPK cascade.
Biochemical and biophysical research communications381(1)75-80(2009 Mar)
Kimura I,Konishi M,Asaki T,Furukawa N,Ukai K,Mori M,Hirasawa A,Tsujimoto G,Ohta M,Itoh N,Fujimoto M

Neurotrophic activity of neudesin, a novel extracellular heme-binding protein, is dependent on the binding of heme to its cytochrome b5-like heme/steroid-binding domain.
The Journal of biological chemistry283(7)4323-31(2008 Feb)
Kimura I,Nakayama Y,Yamauchi H,Konishi M,Miyake A,Mori M,Ohta M,Itoh N,Fujimoto M