CHD5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHD5
Entrez Gene ID	26038
Full Name	chromodomain helicase DNA binding protein 5
Synonyms	CHD-5
General protein information	Preferred Names chromodomain helicase DNA binding protein 5 Names chromodomain-helicase-DNA-binding protein 5 ATP-dependent helicase CHD5
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.31
Summary	This gene encodes a member of the chromodomain helicase DNA-binding protein family. Members of this family are characterized by a chromodomain, a helicase ATP-binding domain and an additional functional domain. This gene encodes a neuron-specific protein that may function in chromatin remodeling and gene transcription. This gene is a potential tumor suppressor gene that may play a role in the development of neuroblastoma. [provided by RefSeq, Feb 2012].
Disorder MIM:	610771

mRNA and Protein(s)

mRNA	Protein	Name
NM_015557.2	NP_056372.1	chromodomain-helicase-DNA-binding protein 5
NM_015557.3	NP_056372.1	chromodomain-helicase-DNA-binding protein 5

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CHD5	XP_002694217.3
Mus musculus (house mouse)	Chd5	NP_001074845.1
Drosophila melanogaster (fruit fly)	Mi-2	NP_001014591.1
Homo sapiens (human)	CHD5	NP_056372.1
Rattus norvegicus (Norway rat)	Chd5	NP_001258155.1
Gallus gallus (chicken)	CHD5	XP_003642567.2
Danio rerio (zebrafish)	chd5	XP_003199545.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012009	XP_320523.4
Canis lupus familiaris (dog)	CHD5	XP_005620504.1
Xenopus tropicalis (tropical clawed frog)	chd5	XP_002937591.2

Gene Ontology
Bibliography

Related articles in PubMed

Histone deacetylase 3 is associated with gastric cancer cell growth via the miR-454-mediated targeting of CHD5.
Xu G, Zhu H, Zhang M, Xu J
International journal of molecular medicine41(1)155-163(2018 Jan)

CHD5 is a potential tumor suppressor in non small cell lung cancer (NSCLC).
Baykara O, Tansarikaya M, Bulut P, Demirkaya A, Buyru N
Gene61865-68(2017 Jun)

The epigenetic modifier CHD5 functions as a novel tumor suppressor for renal cell carcinoma and is predominantly inactivated by promoter CpG methylation.
Du Z, Li L, Huang X, Jin J, Huang S, Zhang Q, Tao Q
Oncotarget7(16)21618-30(2016 Apr)

Influence of colorectal cancer tumor suppressor gene CHD5 methylation on its clinical and pathological characteristics.
Liu JB, Zhou QB, Xu JZ, Wang GX, Yuan WT
Journal of biological regulators and homeostatic agents29(4)889-93(2015 Oct-Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CHD5 may act as a tumor suppressor gene in NSCLC.
Title: CHD5 is a potential tumor suppressor in non small cell lung cancer (NSCLC).

Data show that chromodomain helicase DNA binding protein 5 (CHD5) acted as a functional tumor suppressor and was frequently silenced by promoter CpG methylation in renal cell carcinoma (RCC).
Title: The epigenetic modifier CHD5 functions as a novel tumor suppressor for renal cell carcinoma and is predominantly inactivated by promoter CpG methylation.

Data show a mutual suppression regulation between chromodomain helicase DNA binding protein 5 (CHD5) and enhancer of zeste homolog 2 (EZH2, which may provide new insights into their potential therapeutic significance for hepatocellular carcinoma (HCC).
Title: Mutual regulation between CHD5 and EZH2 in hepatocellular carcinoma.

Authors identified the CHD5 as a direct target of miR-454. CHD5 was downregulated in HCC tissues and cell lines and the expression level of CHD5 was inversely correlated with the expression of miR-454 in HCC tissues.
Title: miR-454 functions as an oncogene by inhibiting CHD5 in hepatocellular carcinoma.

CHD5 methylation of rectal cancer has a great effect in influencing its clinical and pathological features.
Title: Influence of colorectal cancer tumor suppressor gene CHD5 methylation on its clinical and pathological characteristics.

The following CHD5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHD5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu11239	NM_015557.3 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 5 (CHD5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
View Old Accession Versions >>
OHu11239	NM_015557.2	Homo sapiens chromodomain helicase DNA binding protein 5 (CHD5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHD5 ( NM_015557.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_056372.1 Homo sapiens chromodomain helicase DNA binding protein 5 (CHD5), mRNA.

Price & Availability↑

CloneID	OHu11239
Clone ID Related Accession (Same CDS sequence)	NM_015557.2 , NM_015557.3
Accession Version	NM_015557.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL031847.17, AW005809.1, AF425231.1 and AL035406.25. On Nov 22, 2018 this sequence version replaced NM_015557.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF425231.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000262450.8/ ENSP00000262450.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGGGGCC CAGTGGGCAC CGAGGAGGAG CTGCCGCGGC TGTTCGCCGA GGAGATGGAG 
AATGAGGACG AGATGTCAGA AGAAGAAGAT GGTGGTCTTG AAGCCTTCGA TGACTTTTTC 
CCTGTGGAGC CCGTGAGCCT TCCTAAGAAG AAGAAACCCA AGAAGCTCAA GGAAAACAAG 
TGTAAAGGGA AGCGGAAGAA GAAAGAGGGG AGCAATGATG AGCTATCAGA GAATGAAGAG 
GATCTGGAAG AGAAGTCGGA GAGTGAAGGC AGTGACTACT CCCCGAATAA AAAGAAGAAG 
AAGAAACTCA AGGACAAGAA GGAGAAAAAA GCCAAGCGAA AAAAGAAGGA TGAGGATGAG 
GATGATAATG ATGATGGATG CTTAAAGGAG CCCAAGTCCT CGGGGCAGCT CATGGCCGAG 
TGGGGCCTGG ACGACGTGGA CTACCTGTTC TCGGAGGAGG ATTACCACAC GCTGACCAAC 
TACAAGGCCT TCAGCCAGTT CCTCAGGCCA CTCATTGCCA AGAAGAACCC GAAGATCCCC 
ATGTCCAAAA TGATGACCGT CCTGGGTGCC AAGTGGCGGG AGTTCAGCGC CAACAACCCC 
TTCAAGGGCA GCTCCGCGGC AGCAGCGGCG GCGGCGGTGG CTGCGGCTGT AGAGACGGTC 
ACCATCTCCC CTCCGCTAGC CGTCAGCCCC CCGCAGGTGC CCCAGCCTGT GCCTATCCGC 
AAGGCCAAGA CCAAGGAGGG CAAAGGGCCT GGAGTGAGGA AGAAGATCAA AGGCTCCAAA 
GATGGGAAGA AAAAGGGCAA AGGGAAAAAG ACGGCCGGGC TCAAGTTCCG CTTCGGGGGG 
ATCAGCAACA AGAGGAAGAA AGGCTCCTCG AGTGAAGAAG ATGAGAGGGA GGAGTCGGAC 
TTCGACAGCG CCAGCATCCA CAGTGCCTCC GTGCGCTCCG AATGCTCTGC AGCCCTGGGC 
AAGAAGAGCA AGAGGAGGCG CAAGAAGAAG AGGATTGATG ATGGTGACGG CTATGAGACA 
GACCACCAGG ATTACTGTGA GGTGTGCCAG CAGGGTGGGG AGATCATCCT GTGCGACACC 
TGCCCGAGGG CCTACCATCT CGTATGCCTG GACCCAGAGC TGGAGAAGGC TCCCGAGGGC 
AAGTGGAGCT GCCCCCACTG TGAGAAGGAG GGGATCCAGT GGGAGCCGAA GGACGACGAC 
GATGAAGAGG AGGAGGGCGG CTGCGAGGAG GAGGAGGACG ACCACATGGA GTTCTGCCGC 
GTGTGCAAGG ACGGGGGCGA GCTGCTCTGC TGCGACGCCT GCCCCTCCTC CTACCACCTG 
CATTGCCTCA ACCCGCCGCT GCCCGAGATC CCAAACGGTG AATGGCTCTG CCCGCGCTGT 
ACTTGCCCCC CACTGAAGGG CAAAGTCCAG CGGATTCTAC ACTGGAGGTG GACGGAGCCC 
CCTGCCCCCT TCATGGTGGG GCTGCCGGGG CCTGACGTGG AGCCCAGCCT CCCTCCACCT 
AAGCCCCTGG AGGGCATCCC TGAGAGAGAG TTCTTTGTCA AGTGGGCAGG GCTGTCCTAC 
TGGCATTGCT CCTGGGTGAA GGAGCTACAG CTGGAGCTGT ACCACACGGT GATGTATCGC 
AACTACCAAA GAAAGAACGA CATGGATGAG CCGCCCCCCT TTGACTACGG CTCTGGGGAT 
GAAGACGGCA AGAGCGAGAA GAGGAAGAAC AAGGACCCCC TCTATGCCAA GATGGAGGAG 
CGCTTCTACC GCTATGGCAT CAAGCCAGAG TGGATGATGA TTCACCGAAT CCTGAACCAT 
AGCTTTGACA AGAAGGGGGA TGTGCACTAC CTGATCAAGT GGAAAGACCT GCCCTACGAC 
CAGTGCACCT GGGAGATCGA TGACATCGAC ATCCCCTACT ACGACAACCT CAAGCAGGCC 
TACTGGGGCC ACAGGGAGCT GATGCTGGGA GAAGACACCA GGCTGCCCAA GAGGCTGCTC 
AAGAAGGGCA AGAAGCTGAG GGACGACAAG CAGGAGAAGC CGCCGGACAC GCCCATTGTG 
GACCCCACGG TCAAGTTCGA CAAGCAGCCA TGGTACATCG ACTCCACAGG CGGCACACTG 
CACCCGTACC AGCTGGAGGG CCTCAACTGG CTGCGCTTCT CTTGGGCCCA GGGCACTGAC 
ACCATCCTGG CCGATGAGAT GGGTCTGGGC AAGACGGTGC AGACCATCGT GTTCCTTTAC 
TCCCTCTACA AGGAGGGCCA CTCCAAAGGG CCCTACCTGG TTAGCGCGCC CCTCTCCACC 
ATCATCAACT GGGAACGCGA GTTTGAGATG TGGGCGCCCG ACTTCTACGT GGTCACCTAC 
ACGGGGGACA AGGAGAGCCG CTCGGTGATT CGGGAGAACG AGTTTTCCTT TGAGGACAAC 
GCCATTCGGA GTGGGAAGAA GGTATTCCGT ATGAAGAAAG AAGTGCAGAT CAAATTCCAC 
GTGCTGCTCA CCTCCTATGA GCTCATCACC ATTGACCAGG CCATCCTGGG CTCCATCGAG 
TGGGCCTGCC TGGTGGTAGA TGAGGCCCAC CGCCTCAAGA ACAACCAGTC CAAGTTTTTT 
AGGGTCTTAA ACAGCTACAA GATTGATTAC AAGCTGCTGC TGACAGGGAC CCCCCTTCAG 
AACAACCTGG AGGAGCTGTT CCATCTCCTC AACTTCCTGA CTCCAGAGAG GTTCAACAAC 
CTGGAGGGCT TCCTGGAGGA GTTTGCTGAC ATCTCCAAGG AAGACCAGAT CAAGAAGCTG 
CATGACCTGC TGGGGCCGCA CATGCTCAGG CGGCTCAAGG CTGACGTGTT CAAGAACATG 
CCGGCCAAGA CCGAGCTCAT TGTCCGGGTG GAGCTGAGCC AGATGCAGAA GAAGTACTAC 
AAGTTCATCC TCACACGGAA CTTTGAGGCA CTGAACTCCA AGGGGGGCGG GAACCAAGTA 
TCGCTGCTCA ACATCATGAT GGACCTGAAA AAGTGCTGCA ACCACCCCTA CCTCTTCCCT 
GTGGCTGCCG TGGAGGCCCC TGTCTTGCCC AATGGCTCCT ACGATGGAAG CTCCCTGGTC 
AAGTCTTCAG GGAAGCTCAT GCTGCTACAG AAGATGCTGA AGAAACTGCG GGATGAGGGG 
CACCGTGTGC TCATCTTCTC CCAGATGACC AAGATGCTGG ACCTCCTGGA GGACTTCCTG 
GAGTACGAAG GCTACAAGTA TGAGCGGATT GATGGTGGCA TCACCGGGGG CCTCCGGCAG 
GAGGCAATCG ACAGATTCAA TGCCCCCGGG GCCCAGCAGT TCTGCTTCCT CCTCTCAACC 
CGGGCAGGTG GTCTGGGCAT CAACCTGGCC ACGGCGGACA CTGTCATCAT CTACGACTCG 
GACTGGAACC CGCACAATGA CATCCAGGCC TTCAGCCGCG CCCACCGCAT CGGCCAGAAC 
AAGAAGGTGA TGATCTACCG CTTCGTGACT CGGGCCTCGG TGGAGGAGCG CATCACGCAG 
GTGGCCAAGC GCAAGATGAT GCTCACCCAC CTGGTGGTGC GGCCCGGCCT CGGCTCCAAG 
TCGGGGTCCA TGACCAAGCA GGAGCTGGAC GACATCCTCA AGTTCGGCAC GGAGGAACTC 
TTCAAGGACG ACGTGGAGGG CATGATGTCT CAGGGCCAGA GGCCGGTCAC ACCCATCCCT 
GATGTCCAGT CCTCCAAAGG GGGGAACTTG GCCGCCAGTG CAAAGAAGAA GCACGGTAGC 
ACCCCGCCAG GTGACAACAA GGACGTGGAG GACAGCAGTG TGATCCACTA TGACGATGCG 
GCCATCTCCA AGCTGCTGGA CCGGAACCAG GACGCTACAG ATGACACGGA GCTACAGAAC 
ATGAACGAGT ACCTGAGCTC CTTCAAGGTG GCGCAGTACG TGGTGCGCGA GGAGGACGGC 
GTGGAGGAGG TGGAGCGGGA AATCATCAAG CAGGAGGAGA ACGTGGACCC CGACTACTGG 
GAGAAGCTGC TGCGGCACCA CTATGAGCAG CAGCAGGAGG ACCTGGCCCG CAACCTGGGC 
AAGGGCAAGC GCATCCGCAA GCAGGTCAAC TACAACGATG CCTCCCAGGA GGACCAGGAG 
TGGCAGGATG AGCTCTCTGA TAACCAGTCA GAATATTCCA TTGGCTCTGA GGATGAGGAT 
GAGGACTTTG AAGAGAGGCC GGAAGGGCAG AGTGGACGAC GACAATCCCG GAGGCAGCTG 
AAGAGTGACA GGGACAAGCC CCTGCCCCCG CTTCTCGCCC GAGTTGGTGG CAACATCGAG 
GTGCTGGGCT TCAATGCCCG ACAGCGGAAG GCCTTTCTGA ACGCCATCAT GCGCTGGGGC 
ATGCCCCCGC AGGACGCCTT CAACTCCCAC TGGCTGGTGC GGGACCTTCG AGGGAAGAGC 
GAGAAGGAGT TTAGAGCCTA TGTGTCCCTC TTCATGCGGC ACCTGTGTGA GCCGGGGGCG 
GATGGTGCAG AGACCTTCGC AGACGGCGTG CCCCGGGAGG GCCTCTCCAG GCAGCACGTG 
CTGACCCGCA TCGGGGTCAT GTCACTAGTT AGGAAGAAGG TTCAGGAGTT TGAGCATGTC 
AACGGGAAGT ACAGCACCCC AGACTTGATC CCTGAGGGGC CCGAGGGGAA GAAGTCGGGC 
GAGGTGATCT CCTCGGACCC CAACACACCA GTGCCCGCCA GCCCTGCCCA CCTCCTGCCA 
GCCCCGCTGG GCCTGCCAGA CAAAATGGAA GCCCAGCTGG GCTACATGGA TGAGAAAGAC 
CCCGGGGCAC AGAAGCCAAG GCAGCCCCTG GAAGTCCAGG CCCTTCCAGC CGCCTTGGAT 
AGAGTGGAGA GTGAGGACAA GCACGAGAGC CCAGCCAGCA AGGAGAGAGC CCGAGAGGAG 
CGGCCAGAGG AGACGGAGAA GGCCCCGCCC TCCCCGGAGC AGCTGCCGAG AGAGGAGGTG 
CTTCCTGAGA AGGAGAAGAT CCTGGACAAG CTGGAGCTGA GCTTGATCCA CAGCAGAGGG 
GACAGTTCCG AACTCAGGCC AGATGACACC AAGGCTGAGG AGAAGGAGCC CATTGAAACA 
CAGCAAAATG GTGACAAAGA GGAAGATGAC GAGGGGAAGA AGGAGGACAA GAAGGGGAAA 
TTCAAGTTCA TGTTCAACAT CGCGGACGGG GGCTTCACGG AGTTGCACAC GCTGTGGCAG 
AACGAGGAGC GGGCTGCTGT ATCCTCTGGG AAAATCTACG ACATCTGGCA CCGGCGCCAT 
GACTACTGGC TGCTGGCGGG CATCGTGACG CACGGCTACG CCCGCTGGCA GGACATCCAG 
AATGACCCAC GGTACATGAT CCTCAACGAG CCCTTCAAGT CTGAGGTCCA CAAGGGCAAC 
TACCTGGAGA TGAAGAACAA GTTCCTGGCC CGCAGGTTTA AGCTGCTGGA GCAGGCGTTG 
GTCATTGAGG AGCAGCTCCG GAGGGCCGCG TACCTGAACA TGACGCAGGA CCCCAACCAC 
CCCGCCATGG CCCTCAACGC CCGCCTGGCT GAAGTGGAGT GCCTCGCCGA GAGCCACCAG 
CACCTGTCCA AGGAGTCCCT TGCTGGGAAC AAGCCTGCCA ATGCCGTCCT GCACAAGGTC 
CTGAACCAGC TGGAGGAGCT GCTGAGCGAC ATGAAGGCCG ACGTGACCCG GCTGCCATCC 
ATGCTGTCCC GCATCCCCCC GGTGGCCGCC CGGCTGCAGA TGTCGGAGCG CAGCATCCTG 
AGCCGCCTGA CCAACCGCGC CGGGGACCCC ACCATCCAGC AGGGCGCTTT CGGCTCCTCC 
CAGATGTACA GCAACAACTT TGGGCCCAAC TTCCGGGGCC CTGGACCGGG AGGGATTGTC 
AACTACAACC AGATGCCCCT GGGGCCCTAT GTGACCGATA TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056372.1
CDS	299..6163
Translation	MRGPVGTEEELPRLFAEEMENEDEMSEEEDGGLEAFDDFFPVEPVSLPKKKKPKKLKENKCKGKRKKKEGSNDELSENEEDLEEKSESEGSDYSPNKKKKKKLKDKKEKKAKRKKKDEDEDDNDDGCLKEPKSSGQLMAEWGLDDVDYLFSEEDYHTLTNYKAFSQFLRPLIAKKNPKIPMSKMMTVLGAKWREFSANNPFKGSSAAAAAAAVAAAVETVTISPPLAVSPPQVPQPVPIRKAKTKEGKGPGVRKKIKGSKDGKKKGKGKKTAGLKFRFGGISNKRKKGSSSEEDEREESDFDSASIHSASVRSECSAALGKKSKRRRKKKRIDDGDGYETDHQDYCEVCQQGGEIILCDTCPRAYHLVCLDPELEKAPEGKWSCPHCEKEGIQWEPKDDDDEEEEGGCEEEEDDHMEFCRVCKDGGELLCCDACPSSYHLHCLNPPLPEIPNGEWLCPRCTCPPLKGKVQRILHWRWTEPPAPFMVGLPGPDVEPSLPPPKPLEGIPEREFFVKWAGLSYWHCSWVKELQLELYHTVMYRNYQRKNDMDEPPPFDYGSGDEDGKSEKRKNKDPLYAKMEERFYRYGIKPEWMMIHRILNHSFDKKGDVHYLIKWKDLPYDQCTWEIDDIDIPYYDNLKQAYWGHRELMLGEDTRLPKRLLKKGKKLRDDKQEKPPDTPIVDPTVKFDKQPWYIDSTGGTLHPYQLEGLNWLRFSWAQGTDTILADEMGLGKTVQTIVFLYSLYKEGHSKGPYLVSAPLSTIINWEREFEMWAPDFYVVTYTGDKESRSVIRENEFSFEDNAIRSGKKVFRMKKEVQIKFHVLLTSYELITIDQAILGSIEWACLVVDEAHRLKNNQSKFFRVLNSYKIDYKLLLTGTPLQNNLEELFHLLNFLTPERFNNLEGFLEEFADISKEDQIKKLHDLLGPHMLRRLKADVFKNMPAKTELIVRVELSQMQKKYYKFILTRNFEALNSKGGGNQVSLLNIMMDLKKCCNHPYLFPVAAVEAPVLPNGSYDGSSLVKSSGKLMLLQKMLKKLRDEGHRVLIFSQMTKMLDLLEDFLEYEGYKYERIDGGITGGLRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKRKMMLTHLVVRPGLGSKSGSMTKQELDDILKFGTEELFKDDVEGMMSQGQRPVTPIPDVQSSKGGNLAASAKKKHGSTPPGDNKDVEDSSVIHYDDAAISKLLDRNQDATDDTELQNMNEYLSSFKVAQYVVREEDGVEEVEREIIKQEENVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDASQEDQEWQDELSDNQSEYSIGSEDEDEDFEERPEGQSGRRQSRRQLKSDRDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRWGMPPQDAFNSHWLVRDLRGKSEKEFRAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLVRKKVQEFEHVNGKYSTPDLIPEGPEGKKSGEVISSDPNTPVPASPAHLLPAPLGLPDKMEAQLGYMDEKDPGAQKPRQPLEVQALPAALDRVESEDKHESPASKERAREERPEETEKAPPSPEQLPREEVLPEKEKILDKLELSLIHSRGDSSELRPDDTKAEEKEPIETQQNGDKEEDDEGKKEDKKGKFKFMFNIADGGFTELHTLWQNEERAAVSSGKIYDIWHRRHDYWLLAGIVTHGYARWQDIQNDPRYMILNEPFKSEVHKGNYLEMKNKFLARRFKLLEQALVIEEQLRRAAYLNMTQDPNHPAMALNARLAEVECLAESHQHLSKESLAGNKPANAVLHKVLNQLEELLSDMKADVTRLPSMLSRIPPVAARLQMSERSILSRLTNRAGDPTIQQGAFGSSQMYSNNFGPNFRGPGPGGIVNYNQMPLGPYVTDI

Target ORF information:

RefSeq Version	NM_015557.3
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 5 (CHD5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015557.3

ORF Insert Sequence:

ATGCGGGGCC CAGTGGGCAC CGAGGAGGAG CTGCCGCGGC TGTTCGCCGA GGAGATGGAG 
AATGAGGACG AGATGTCAGA AGAAGAAGAT GGTGGTCTTG AAGCCTTCGA TGACTTTTTC 
CCTGTGGAGC CCGTGAGCCT TCCTAAGAAG AAGAAACCCA AGAAGCTCAA GGAAAACAAG 
TGTAAAGGGA AGCGGAAGAA GAAAGAGGGG AGCAATGATG AGCTATCAGA GAATGAAGAG 
GATCTGGAAG AGAAGTCGGA GAGTGAAGGC AGTGACTACT CCCCGAATAA AAAGAAGAAG 
AAGAAACTCA AGGACAAGAA GGAGAAAAAA GCCAAGCGAA AAAAGAAGGA TGAGGATGAG 
GATGATAATG ATGATGGATG CTTAAAGGAG CCCAAGTCCT CGGGGCAGCT CATGGCCGAG 
TGGGGCCTGG ACGACGTGGA CTACCTGTTC TCGGAGGAGG ATTACCACAC GCTGACCAAC 
TACAAGGCCT TCAGCCAGTT CCTCAGGCCA CTCATTGCCA AGAAGAACCC GAAGATCCCC 
ATGTCCAAAA TGATGACCGT CCTGGGTGCC AAGTGGCGGG AGTTCAGCGC CAACAACCCC 
TTCAAGGGCA GCTCCGCGGC AGCAGCGGCG GCGGCGGTGG CTGCGGCTGT AGAGACGGTC 
ACCATCTCCC CTCCGCTAGC CGTCAGCCCC CCGCAGGTGC CCCAGCCTGT GCCTATCCGC 
AAGGCCAAGA CCAAGGAGGG CAAAGGGCCT GGAGTGAGGA AGAAGATCAA AGGCTCCAAA 
GATGGGAAGA AAAAGGGCAA AGGGAAAAAG ACGGCCGGGC TCAAGTTCCG CTTCGGGGGG 
ATCAGCAACA AGAGGAAGAA AGGCTCCTCG AGTGAAGAAG ATGAGAGGGA GGAGTCGGAC 
TTCGACAGCG CCAGCATCCA CAGTGCCTCC GTGCGCTCCG AATGCTCTGC AGCCCTGGGC 
AAGAAGAGCA AGAGGAGGCG CAAGAAGAAG AGGATTGATG ATGGTGACGG CTATGAGACA 
GACCACCAGG ATTACTGTGA GGTGTGCCAG CAGGGTGGGG AGATCATCCT GTGCGACACC 
TGCCCGAGGG CCTACCATCT CGTATGCCTG GACCCAGAGC TGGAGAAGGC TCCCGAGGGC 
AAGTGGAGCT GCCCCCACTG TGAGAAGGAG GGGATCCAGT GGGAGCCGAA GGACGACGAC 
GATGAAGAGG AGGAGGGCGG CTGCGAGGAG GAGGAGGACG ACCACATGGA GTTCTGCCGC 
GTGTGCAAGG ACGGGGGCGA GCTGCTCTGC TGCGACGCCT GCCCCTCCTC CTACCACCTG 
CATTGCCTCA ACCCGCCGCT GCCCGAGATC CCAAACGGTG AATGGCTCTG CCCGCGCTGT 
ACTTGCCCCC CACTGAAGGG CAAAGTCCAG CGGATTCTAC ACTGGAGGTG GACGGAGCCC 
CCTGCCCCCT TCATGGTGGG GCTGCCGGGG CCTGACGTGG AGCCCAGCCT CCCTCCACCT 
AAGCCCCTGG AGGGCATCCC TGAGAGAGAG TTCTTTGTCA AGTGGGCAGG GCTGTCCTAC 
TGGCATTGCT CCTGGGTGAA GGAGCTACAG CTGGAGCTGT ACCACACGGT GATGTATCGC 
AACTACCAAA GAAAGAACGA CATGGATGAG CCGCCCCCCT TTGACTACGG CTCTGGGGAT 
GAAGACGGCA AGAGCGAGAA GAGGAAGAAC AAGGACCCCC TCTATGCCAA GATGGAGGAG 
CGCTTCTACC GCTATGGCAT CAAGCCAGAG TGGATGATGA TTCACCGAAT CCTGAACCAT 
AGCTTTGACA AGAAGGGGGA TGTGCACTAC CTGATCAAGT GGAAAGACCT GCCCTACGAC 
CAGTGCACCT GGGAGATCGA TGACATCGAC ATCCCCTACT ACGACAACCT CAAGCAGGCC 
TACTGGGGCC ACAGGGAGCT GATGCTGGGA GAAGACACCA GGCTGCCCAA GAGGCTGCTC 
AAGAAGGGCA AGAAGCTGAG GGACGACAAG CAGGAGAAGC CGCCGGACAC GCCCATTGTG 
GACCCCACGG TCAAGTTCGA CAAGCAGCCA TGGTACATCG ACTCCACAGG CGGCACACTG 
CACCCGTACC AGCTGGAGGG CCTCAACTGG CTGCGCTTCT CTTGGGCCCA GGGCACTGAC 
ACCATCCTGG CCGATGAGAT GGGTCTGGGC AAGACGGTGC AGACCATCGT GTTCCTTTAC 
TCCCTCTACA AGGAGGGCCA CTCCAAAGGG CCCTACCTGG TTAGCGCGCC CCTCTCCACC 
ATCATCAACT GGGAACGCGA GTTTGAGATG TGGGCGCCCG ACTTCTACGT GGTCACCTAC 
ACGGGGGACA AGGAGAGCCG CTCGGTGATT CGGGAGAACG AGTTTTCCTT TGAGGACAAC 
GCCATTCGGA GTGGGAAGAA GGTATTCCGT ATGAAGAAAG AAGTGCAGAT CAAATTCCAC 
GTGCTGCTCA CCTCCTATGA GCTCATCACC ATTGACCAGG CCATCCTGGG CTCCATCGAG 
TGGGCCTGCC TGGTGGTAGA TGAGGCCCAC CGCCTCAAGA ACAACCAGTC CAAGTTTTTT 
AGGGTCTTAA ACAGCTACAA GATTGATTAC AAGCTGCTGC TGACAGGGAC CCCCCTTCAG 
AACAACCTGG AGGAGCTGTT CCATCTCCTC AACTTCCTGA CTCCAGAGAG GTTCAACAAC 
CTGGAGGGCT TCCTGGAGGA GTTTGCTGAC ATCTCCAAGG AAGACCAGAT CAAGAAGCTG 
CATGACCTGC TGGGGCCGCA CATGCTCAGG CGGCTCAAGG CTGACGTGTT CAAGAACATG 
CCGGCCAAGA CCGAGCTCAT TGTCCGGGTG GAGCTGAGCC AGATGCAGAA GAAGTACTAC 
AAGTTCATCC TCACACGGAA CTTTGAGGCA CTGAACTCCA AGGGGGGCGG GAACCAAGTA 
TCGCTGCTCA ACATCATGAT GGACCTGAAA AAGTGCTGCA ACCACCCCTA CCTCTTCCCT 
GTGGCTGCCG TGGAGGCCCC TGTCTTGCCC AATGGCTCCT ACGATGGAAG CTCCCTGGTC 
AAGTCTTCAG GGAAGCTCAT GCTGCTACAG AAGATGCTGA AGAAACTGCG GGATGAGGGG 
CACCGTGTGC TCATCTTCTC CCAGATGACC AAGATGCTGG ACCTCCTGGA GGACTTCCTG 
GAGTACGAAG GCTACAAGTA TGAGCGGATT GATGGTGGCA TCACCGGGGG CCTCCGGCAG 
GAGGCAATCG ACAGATTCAA TGCCCCCGGG GCCCAGCAGT TCTGCTTCCT CCTCTCAACC 
CGGGCAGGTG GTCTGGGCAT CAACCTGGCC ACGGCGGACA CTGTCATCAT CTACGACTCG 
GACTGGAACC CGCACAATGA CATCCAGGCC TTCAGCCGCG CCCACCGCAT CGGCCAGAAC 
AAGAAGGTGA TGATCTACCG CTTCGTGACT CGGGCCTCGG TGGAGGAGCG CATCACGCAG 
GTGGCCAAGC GCAAGATGAT GCTCACCCAC CTGGTGGTGC GGCCCGGCCT CGGCTCCAAG 
TCGGGGTCCA TGACCAAGCA GGAGCTGGAC GACATCCTCA AGTTCGGCAC GGAGGAACTC 
TTCAAGGACG ACGTGGAGGG CATGATGTCT CAGGGCCAGA GGCCGGTCAC ACCCATCCCT 
GATGTCCAGT CCTCCAAAGG GGGGAACTTG GCCGCCAGTG CAAAGAAGAA GCACGGTAGC 
ACCCCGCCAG GTGACAACAA GGACGTGGAG GACAGCAGTG TGATCCACTA TGACGATGCG 
GCCATCTCCA AGCTGCTGGA CCGGAACCAG GACGCTACAG ATGACACGGA GCTACAGAAC 
ATGAACGAGT ACCTGAGCTC CTTCAAGGTG GCGCAGTACG TGGTGCGCGA GGAGGACGGC 
GTGGAGGAGG TGGAGCGGGA AATCATCAAG CAGGAGGAGA ACGTGGACCC CGACTACTGG 
GAGAAGCTGC TGCGGCACCA CTATGAGCAG CAGCAGGAGG ACCTGGCCCG CAACCTGGGC 
AAGGGCAAGC GCATCCGCAA GCAGGTCAAC TACAACGATG CCTCCCAGGA GGACCAGGAG 
TGGCAGGATG AGCTCTCTGA TAACCAGTCA GAATATTCCA TTGGCTCTGA GGATGAGGAT 
GAGGACTTTG AAGAGAGGCC GGAAGGGCAG AGTGGACGAC GACAATCCCG GAGGCAGCTG 
AAGAGTGACA GGGACAAGCC CCTGCCCCCG CTTCTCGCCC GAGTTGGTGG CAACATCGAG 
GTGCTGGGCT TCAATGCCCG ACAGCGGAAG GCCTTTCTGA ACGCCATCAT GCGCTGGGGC 
ATGCCCCCGC AGGACGCCTT CAACTCCCAC TGGCTGGTGC GGGACCTTCG AGGGAAGAGC 
GAGAAGGAGT TTAGAGCCTA TGTGTCCCTC TTCATGCGGC ACCTGTGTGA GCCGGGGGCG 
GATGGTGCAG AGACCTTCGC AGACGGCGTG CCCCGGGAGG GCCTCTCCAG GCAGCACGTG 
CTGACCCGCA TCGGGGTCAT GTCACTAGTT AGGAAGAAGG TTCAGGAGTT TGAGCATGTC 
AACGGGAAGT ACAGCACCCC AGACTTGATC CCTGAGGGGC CCGAGGGGAA GAAGTCGGGC 
GAGGTGATCT CCTCGGACCC CAACACACCA GTGCCCGCCA GCCCTGCCCA CCTCCTGCCA 
GCCCCGCTGG GCCTGCCAGA CAAAATGGAA GCCCAGCTGG GCTACATGGA TGAGAAAGAC 
CCCGGGGCAC AGAAGCCAAG GCAGCCCCTG GAAGTCCAGG CCCTTCCAGC CGCCTTGGAT 
AGAGTGGAGA GTGAGGACAA GCACGAGAGC CCAGCCAGCA AGGAGAGAGC CCGAGAGGAG 
CGGCCAGAGG AGACGGAGAA GGCCCCGCCC TCCCCGGAGC AGCTGCCGAG AGAGGAGGTG 
CTTCCTGAGA AGGAGAAGAT CCTGGACAAG CTGGAGCTGA GCTTGATCCA CAGCAGAGGG 
GACAGTTCCG AACTCAGGCC AGATGACACC AAGGCTGAGG AGAAGGAGCC CATTGAAACA 
CAGCAAAATG GTGACAAAGA GGAAGATGAC GAGGGGAAGA AGGAGGACAA GAAGGGGAAA 
TTCAAGTTCA TGTTCAACAT CGCGGACGGG GGCTTCACGG AGTTGCACAC GCTGTGGCAG 
AACGAGGAGC GGGCTGCTGT ATCCTCTGGG AAAATCTACG ACATCTGGCA CCGGCGCCAT 
GACTACTGGC TGCTGGCGGG CATCGTGACG CACGGCTACG CCCGCTGGCA GGACATCCAG 
AATGACCCAC GGTACATGAT CCTCAACGAG CCCTTCAAGT CTGAGGTCCA CAAGGGCAAC 
TACCTGGAGA TGAAGAACAA GTTCCTGGCC CGCAGGTTTA AGCTGCTGGA GCAGGCGTTG 
GTCATTGAGG AGCAGCTCCG GAGGGCCGCG TACCTGAACA TGACGCAGGA CCCCAACCAC 
CCCGCCATGG CCCTCAACGC CCGCCTGGCT GAAGTGGAGT GCCTCGCCGA GAGCCACCAG 
CACCTGTCCA AGGAGTCCCT TGCTGGGAAC AAGCCTGCCA ATGCCGTCCT GCACAAGGTC 
CTGAACCAGC TGGAGGAGCT GCTGAGCGAC ATGAAGGCCG ACGTGACCCG GCTGCCATCC 
ATGCTGTCCC GCATCCCCCC GGTGGCCGCC CGGCTGCAGA TGTCGGAGCG CAGCATCCTG 
AGCCGCCTGA CCAACCGCGC CGGGGACCCC ACCATCCAGC AGGGCGCTTT CGGCTCCTCC 
CAGATGTACA GCAACAACTT TGGGCCCAAC TTCCGGGGCC CTGGACCGGG AGGGATTGTC 
AACTACAACC AGATGCCCCT GGGGCCCTAT GTGACCGATA TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD5 ( NM_015557.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_056372.1 Homo sapiens chromodomain helicase DNA binding protein 5 (CHD5), mRNA.

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CloneID	OHu11239
Clone ID Related Accession (Same CDS sequence)	NM_015557.2 , NM_015557.3
Accession Version	NM_015557.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AW005809.1, AF425231.1 and AL035406.25. On Aug 21, 2010 this sequence version replaced NM_015557.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF425231.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGGGCC CAGTGGGCAC CGAGGAGGAG CTGCCGCGGC TGTTCGCCGA GGAGATGGAG 
AATGAGGACG AGATGTCAGA AGAAGAAGAT GGTGGTCTTG AAGCCTTCGA TGACTTTTTC 
CCTGTGGAGC CCGTGAGCCT TCCTAAGAAG AAGAAACCCA AGAAGCTCAA GGAAAACAAG 
TGTAAAGGGA AGCGGAAGAA GAAAGAGGGG AGCAATGATG AGCTATCAGA GAATGAAGAG 
GATCTGGAAG AGAAGTCGGA GAGTGAAGGC AGTGACTACT CCCCGAATAA AAAGAAGAAG 
AAGAAACTCA AGGACAAGAA GGAGAAAAAA GCCAAGCGAA AAAAGAAGGA TGAGGATGAG 
GATGATAATG ATGATGGATG CTTAAAGGAG CCCAAGTCCT CGGGGCAGCT CATGGCCGAG 
TGGGGCCTGG ACGACGTGGA CTACCTGTTC TCGGAGGAGG ATTACCACAC GCTGACCAAC 
TACAAGGCCT TCAGCCAGTT CCTCAGGCCA CTCATTGCCA AGAAGAACCC GAAGATCCCC 
ATGTCCAAAA TGATGACCGT CCTGGGTGCC AAGTGGCGGG AGTTCAGCGC CAACAACCCC 
TTCAAGGGCA GCTCCGCGGC AGCAGCGGCG GCGGCGGTGG CTGCGGCTGT AGAGACGGTC 
ACCATCTCCC CTCCGCTAGC CGTCAGCCCC CCGCAGGTGC CCCAGCCTGT GCCTATCCGC 
AAGGCCAAGA CCAAGGAGGG CAAAGGGCCT GGAGTGAGGA AGAAGATCAA AGGCTCCAAA 
GATGGGAAGA AAAAGGGCAA AGGGAAAAAG ACGGCCGGGC TCAAGTTCCG CTTCGGGGGG 
ATCAGCAACA AGAGGAAGAA AGGCTCCTCG AGTGAAGAAG ATGAGAGGGA GGAGTCGGAC 
TTCGACAGCG CCAGCATCCA CAGTGCCTCC GTGCGCTCCG AATGCTCTGC AGCCCTGGGC 
AAGAAGAGCA AGAGGAGGCG CAAGAAGAAG AGGATTGATG ATGGTGACGG CTATGAGACA 
GACCACCAGG ATTACTGTGA GGTGTGCCAG CAGGGTGGGG AGATCATCCT GTGCGACACC 
TGCCCGAGGG CCTACCATCT CGTATGCCTG GACCCAGAGC TGGAGAAGGC TCCCGAGGGC 
AAGTGGAGCT GCCCCCACTG TGAGAAGGAG GGGATCCAGT GGGAGCCGAA GGACGACGAC 
GATGAAGAGG AGGAGGGCGG CTGCGAGGAG GAGGAGGACG ACCACATGGA GTTCTGCCGC 
GTGTGCAAGG ACGGGGGCGA GCTGCTCTGC TGCGACGCCT GCCCCTCCTC CTACCACCTG 
CATTGCCTCA ACCCGCCGCT GCCCGAGATC CCAAACGGTG AATGGCTCTG CCCGCGCTGT 
ACTTGCCCCC CACTGAAGGG CAAAGTCCAG CGGATTCTAC ACTGGAGGTG GACGGAGCCC 
CCTGCCCCCT TCATGGTGGG GCTGCCGGGG CCTGACGTGG AGCCCAGCCT CCCTCCACCT 
AAGCCCCTGG AGGGCATCCC TGAGAGAGAG TTCTTTGTCA AGTGGGCAGG GCTGTCCTAC 
TGGCATTGCT CCTGGGTGAA GGAGCTACAG CTGGAGCTGT ACCACACGGT GATGTATCGC 
AACTACCAAA GAAAGAACGA CATGGATGAG CCGCCCCCCT TTGACTACGG CTCTGGGGAT 
GAAGACGGCA AGAGCGAGAA GAGGAAGAAC AAGGACCCCC TCTATGCCAA GATGGAGGAG 
CGCTTCTACC GCTATGGCAT CAAGCCAGAG TGGATGATGA TTCACCGAAT CCTGAACCAT 
AGCTTTGACA AGAAGGGGGA TGTGCACTAC CTGATCAAGT GGAAAGACCT GCCCTACGAC 
CAGTGCACCT GGGAGATCGA TGACATCGAC ATCCCCTACT ACGACAACCT CAAGCAGGCC 
TACTGGGGCC ACAGGGAGCT GATGCTGGGA GAAGACACCA GGCTGCCCAA GAGGCTGCTC 
AAGAAGGGCA AGAAGCTGAG GGACGACAAG CAGGAGAAGC CGCCGGACAC GCCCATTGTG 
GACCCCACGG TCAAGTTCGA CAAGCAGCCA TGGTACATCG ACTCCACAGG CGGCACACTG 
CACCCGTACC AGCTGGAGGG CCTCAACTGG CTGCGCTTCT CTTGGGCCCA GGGCACTGAC 
ACCATCCTGG CCGATGAGAT GGGTCTGGGC AAGACGGTGC AGACCATCGT GTTCCTTTAC 
TCCCTCTACA AGGAGGGCCA CTCCAAAGGG CCCTACCTGG TTAGCGCGCC CCTCTCCACC 
ATCATCAACT GGGAACGCGA GTTTGAGATG TGGGCGCCCG ACTTCTACGT GGTCACCTAC 
ACGGGGGACA AGGAGAGCCG CTCGGTGATT CGGGAGAACG AGTTTTCCTT TGAGGACAAC 
GCCATTCGGA GTGGGAAGAA GGTATTCCGT ATGAAGAAAG AAGTGCAGAT CAAATTCCAC 
GTGCTGCTCA CCTCCTATGA GCTCATCACC ATTGACCAGG CCATCCTGGG CTCCATCGAG 
TGGGCCTGCC TGGTGGTAGA TGAGGCCCAC CGCCTCAAGA ACAACCAGTC CAAGTTTTTT 
AGGGTCTTAA ACAGCTACAA GATTGATTAC AAGCTGCTGC TGACAGGGAC CCCCCTTCAG 
AACAACCTGG AGGAGCTGTT CCATCTCCTC AACTTCCTGA CTCCAGAGAG GTTCAACAAC 
CTGGAGGGCT TCCTGGAGGA GTTTGCTGAC ATCTCCAAGG AAGACCAGAT CAAGAAGCTG 
CATGACCTGC TGGGGCCGCA CATGCTCAGG CGGCTCAAGG CTGACGTGTT CAAGAACATG 
CCGGCCAAGA CCGAGCTCAT TGTCCGGGTG GAGCTGAGCC AGATGCAGAA GAAGTACTAC 
AAGTTCATCC TCACACGGAA CTTTGAGGCA CTGAACTCCA AGGGGGGCGG GAACCAAGTA 
TCGCTGCTCA ACATCATGAT GGACCTGAAA AAGTGCTGCA ACCACCCCTA CCTCTTCCCT 
GTGGCTGCCG TGGAGGCCCC TGTCTTGCCC AATGGCTCCT ACGATGGAAG CTCCCTGGTC 
AAGTCTTCAG GGAAGCTCAT GCTGCTACAG AAGATGCTGA AGAAACTGCG GGATGAGGGG 
CACCGTGTGC TCATCTTCTC CCAGATGACC AAGATGCTGG ACCTCCTGGA GGACTTCCTG 
GAGTACGAAG GCTACAAGTA TGAGCGGATT GATGGTGGCA TCACCGGGGG CCTCCGGCAG 
GAGGCAATCG ACAGATTCAA TGCCCCCGGG GCCCAGCAGT TCTGCTTCCT CCTCTCAACC 
CGGGCAGGTG GTCTGGGCAT CAACCTGGCC ACGGCGGACA CTGTCATCAT CTACGACTCG 
GACTGGAACC CGCACAATGA CATCCAGGCC TTCAGCCGCG CCCACCGCAT CGGCCAGAAC 
AAGAAGGTGA TGATCTACCG CTTCGTGACT CGGGCCTCGG TGGAGGAGCG CATCACGCAG 
GTGGCCAAGC GCAAGATGAT GCTCACCCAC CTGGTGGTGC GGCCCGGCCT CGGCTCCAAG 
TCGGGGTCCA TGACCAAGCA GGAGCTGGAC GACATCCTCA AGTTCGGCAC GGAGGAACTC 
TTCAAGGACG ACGTGGAGGG CATGATGTCT CAGGGCCAGA GGCCGGTCAC ACCCATCCCT 
GATGTCCAGT CCTCCAAAGG GGGGAACTTG GCCGCCAGTG CAAAGAAGAA GCACGGTAGC 
ACCCCGCCAG GTGACAACAA GGACGTGGAG GACAGCAGTG TGATCCACTA TGACGATGCG 
GCCATCTCCA AGCTGCTGGA CCGGAACCAG GACGCTACAG ATGACACGGA GCTACAGAAC 
ATGAACGAGT ACCTGAGCTC CTTCAAGGTG GCGCAGTACG TGGTGCGCGA GGAGGACGGC 
GTGGAGGAGG TGGAGCGGGA AATCATCAAG CAGGAGGAGA ACGTGGACCC CGACTACTGG 
GAGAAGCTGC TGCGGCACCA CTATGAGCAG CAGCAGGAGG ACCTGGCCCG CAACCTGGGC 
AAGGGCAAGC GCATCCGCAA GCAGGTCAAC TACAACGATG CCTCCCAGGA GGACCAGGAG 
TGGCAGGATG AGCTCTCTGA TAACCAGTCA GAATATTCCA TTGGCTCTGA GGATGAGGAT 
GAGGACTTTG AAGAGAGGCC GGAAGGGCAG AGTGGACGAC GACAATCCCG GAGGCAGCTG 
AAGAGTGACA GGGACAAGCC CCTGCCCCCG CTTCTCGCCC GAGTTGGTGG CAACATCGAG 
GTGCTGGGCT TCAATGCCCG ACAGCGGAAG GCCTTTCTGA ACGCCATCAT GCGCTGGGGC 
ATGCCCCCGC AGGACGCCTT CAACTCCCAC TGGCTGGTGC GGGACCTTCG AGGGAAGAGC 
GAGAAGGAGT TTAGAGCCTA TGTGTCCCTC TTCATGCGGC ACCTGTGTGA GCCGGGGGCG 
GATGGTGCAG AGACCTTCGC AGACGGCGTG CCCCGGGAGG GCCTCTCCAG GCAGCACGTG 
CTGACCCGCA TCGGGGTCAT GTCACTAGTT AGGAAGAAGG TTCAGGAGTT TGAGCATGTC 
AACGGGAAGT ACAGCACCCC AGACTTGATC CCTGAGGGGC CCGAGGGGAA GAAGTCGGGC 
GAGGTGATCT CCTCGGACCC CAACACACCA GTGCCCGCCA GCCCTGCCCA CCTCCTGCCA 
GCCCCGCTGG GCCTGCCAGA CAAAATGGAA GCCCAGCTGG GCTACATGGA TGAGAAAGAC 
CCCGGGGCAC AGAAGCCAAG GCAGCCCCTG GAAGTCCAGG CCCTTCCAGC CGCCTTGGAT 
AGAGTGGAGA GTGAGGACAA GCACGAGAGC CCAGCCAGCA AGGAGAGAGC CCGAGAGGAG 
CGGCCAGAGG AGACGGAGAA GGCCCCGCCC TCCCCGGAGC AGCTGCCGAG AGAGGAGGTG 
CTTCCTGAGA AGGAGAAGAT CCTGGACAAG CTGGAGCTGA GCTTGATCCA CAGCAGAGGG 
GACAGTTCCG AACTCAGGCC AGATGACACC AAGGCTGAGG AGAAGGAGCC CATTGAAACA 
CAGCAAAATG GTGACAAAGA GGAAGATGAC GAGGGGAAGA AGGAGGACAA GAAGGGGAAA 
TTCAAGTTCA TGTTCAACAT CGCGGACGGG GGCTTCACGG AGTTGCACAC GCTGTGGCAG 
AACGAGGAGC GGGCTGCTGT ATCCTCTGGG AAAATCTACG ACATCTGGCA CCGGCGCCAT 
GACTACTGGC TGCTGGCGGG CATCGTGACG CACGGCTACG CCCGCTGGCA GGACATCCAG 
AATGACCCAC GGTACATGAT CCTCAACGAG CCCTTCAAGT CTGAGGTCCA CAAGGGCAAC 
TACCTGGAGA TGAAGAACAA GTTCCTGGCC CGCAGGTTTA AGCTGCTGGA GCAGGCGTTG 
GTCATTGAGG AGCAGCTCCG GAGGGCCGCG TACCTGAACA TGACGCAGGA CCCCAACCAC 
CCCGCCATGG CCCTCAACGC CCGCCTGGCT GAAGTGGAGT GCCTCGCCGA GAGCCACCAG 
CACCTGTCCA AGGAGTCCCT TGCTGGGAAC AAGCCTGCCA ATGCCGTCCT GCACAAGGTC 
CTGAACCAGC TGGAGGAGCT GCTGAGCGAC ATGAAGGCCG ACGTGACCCG GCTGCCATCC 
ATGCTGTCCC GCATCCCCCC GGTGGCCGCC CGGCTGCAGA TGTCGGAGCG CAGCATCCTG 
AGCCGCCTGA CCAACCGCGC CGGGGACCCC ACCATCCAGC AGGGCGCTTT CGGCTCCTCC 
CAGATGTACA GCAACAACTT TGGGCCCAAC TTCCGGGGCC CTGGACCGGG AGGGATTGTC 
AACTACAACC AGATGCCCCT GGGGCCCTAT GTGACCGATA TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056372.1
CDS	112..5976
Translation	MRGPVGTEEELPRLFAEEMENEDEMSEEEDGGLEAFDDFFPVEPVSLPKKKKPKKLKENKCKGKRKKKEGSNDELSENEEDLEEKSESEGSDYSPNKKKKKKLKDKKEKKAKRKKKDEDEDDNDDGCLKEPKSSGQLMAEWGLDDVDYLFSEEDYHTLTNYKAFSQFLRPLIAKKNPKIPMSKMMTVLGAKWREFSANNPFKGSSAAAAAAAVAAAVETVTISPPLAVSPPQVPQPVPIRKAKTKEGKGPGVRKKIKGSKDGKKKGKGKKTAGLKFRFGGISNKRKKGSSSEEDEREESDFDSASIHSASVRSECSAALGKKSKRRRKKKRIDDGDGYETDHQDYCEVCQQGGEIILCDTCPRAYHLVCLDPELEKAPEGKWSCPHCEKEGIQWEPKDDDDEEEEGGCEEEEDDHMEFCRVCKDGGELLCCDACPSSYHLHCLNPPLPEIPNGEWLCPRCTCPPLKGKVQRILHWRWTEPPAPFMVGLPGPDVEPSLPPPKPLEGIPEREFFVKWAGLSYWHCSWVKELQLELYHTVMYRNYQRKNDMDEPPPFDYGSGDEDGKSEKRKNKDPLYAKMEERFYRYGIKPEWMMIHRILNHSFDKKGDVHYLIKWKDLPYDQCTWEIDDIDIPYYDNLKQAYWGHRELMLGEDTRLPKRLLKKGKKLRDDKQEKPPDTPIVDPTVKFDKQPWYIDSTGGTLHPYQLEGLNWLRFSWAQGTDTILADEMGLGKTVQTIVFLYSLYKEGHSKGPYLVSAPLSTIINWEREFEMWAPDFYVVTYTGDKESRSVIRENEFSFEDNAIRSGKKVFRMKKEVQIKFHVLLTSYELITIDQAILGSIEWACLVVDEAHRLKNNQSKFFRVLNSYKIDYKLLLTGTPLQNNLEELFHLLNFLTPERFNNLEGFLEEFADISKEDQIKKLHDLLGPHMLRRLKADVFKNMPAKTELIVRVELSQMQKKYYKFILTRNFEALNSKGGGNQVSLLNIMMDLKKCCNHPYLFPVAAVEAPVLPNGSYDGSSLVKSSGKLMLLQKMLKKLRDEGHRVLIFSQMTKMLDLLEDFLEYEGYKYERIDGGITGGLRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKRKMMLTHLVVRPGLGSKSGSMTKQELDDILKFGTEELFKDDVEGMMSQGQRPVTPIPDVQSSKGGNLAASAKKKHGSTPPGDNKDVEDSSVIHYDDAAISKLLDRNQDATDDTELQNMNEYLSSFKVAQYVVREEDGVEEVEREIIKQEENVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDASQEDQEWQDELSDNQSEYSIGSEDEDEDFEERPEGQSGRRQSRRQLKSDRDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRWGMPPQDAFNSHWLVRDLRGKSEKEFRAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLVRKKVQEFEHVNGKYSTPDLIPEGPEGKKSGEVISSDPNTPVPASPAHLLPAPLGLPDKMEAQLGYMDEKDPGAQKPRQPLEVQALPAALDRVESEDKHESPASKERAREERPEETEKAPPSPEQLPREEVLPEKEKILDKLELSLIHSRGDSSELRPDDTKAEEKEPIETQQNGDKEEDDEGKKEDKKGKFKFMFNIADGGFTELHTLWQNEERAAVSSGKIYDIWHRRHDYWLLAGIVTHGYARWQDIQNDPRYMILNEPFKSEVHKGNYLEMKNKFLARRFKLLEQALVIEEQLRRAAYLNMTQDPNHPAMALNARLAEVECLAESHQHLSKESLAGNKPANAVLHKVLNQLEELLSDMKADVTRLPSMLSRIPPVAARLQMSERSILSRLTNRAGDPTIQQGAFGSSQMYSNNFGPNFRGPGPGGIVNYNQMPLGPYVTDI

Target ORF information:

RefSeq Version	NM_015557.2
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 5 (CHD5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015557.2

ORF Insert Sequence:

ATGCGGGGCC CAGTGGGCAC CGAGGAGGAG CTGCCGCGGC TGTTCGCCGA GGAGATGGAG 
AATGAGGACG AGATGTCAGA AGAAGAAGAT GGTGGTCTTG AAGCCTTCGA TGACTTTTTC 
CCTGTGGAGC CCGTGAGCCT TCCTAAGAAG AAGAAACCCA AGAAGCTCAA GGAAAACAAG 
TGTAAAGGGA AGCGGAAGAA GAAAGAGGGG AGCAATGATG AGCTATCAGA GAATGAAGAG 
GATCTGGAAG AGAAGTCGGA GAGTGAAGGC AGTGACTACT CCCCGAATAA AAAGAAGAAG 
AAGAAACTCA AGGACAAGAA GGAGAAAAAA GCCAAGCGAA AAAAGAAGGA TGAGGATGAG 
GATGATAATG ATGATGGATG CTTAAAGGAG CCCAAGTCCT CGGGGCAGCT CATGGCCGAG 
TGGGGCCTGG ACGACGTGGA CTACCTGTTC TCGGAGGAGG ATTACCACAC GCTGACCAAC 
TACAAGGCCT TCAGCCAGTT CCTCAGGCCA CTCATTGCCA AGAAGAACCC GAAGATCCCC 
ATGTCCAAAA TGATGACCGT CCTGGGTGCC AAGTGGCGGG AGTTCAGCGC CAACAACCCC 
TTCAAGGGCA GCTCCGCGGC AGCAGCGGCG GCGGCGGTGG CTGCGGCTGT AGAGACGGTC 
ACCATCTCCC CTCCGCTAGC CGTCAGCCCC CCGCAGGTGC CCCAGCCTGT GCCTATCCGC 
AAGGCCAAGA CCAAGGAGGG CAAAGGGCCT GGAGTGAGGA AGAAGATCAA AGGCTCCAAA 
GATGGGAAGA AAAAGGGCAA AGGGAAAAAG ACGGCCGGGC TCAAGTTCCG CTTCGGGGGG 
ATCAGCAACA AGAGGAAGAA AGGCTCCTCG AGTGAAGAAG ATGAGAGGGA GGAGTCGGAC 
TTCGACAGCG CCAGCATCCA CAGTGCCTCC GTGCGCTCCG AATGCTCTGC AGCCCTGGGC 
AAGAAGAGCA AGAGGAGGCG CAAGAAGAAG AGGATTGATG ATGGTGACGG CTATGAGACA 
GACCACCAGG ATTACTGTGA GGTGTGCCAG CAGGGTGGGG AGATCATCCT GTGCGACACC 
TGCCCGAGGG CCTACCATCT CGTATGCCTG GACCCAGAGC TGGAGAAGGC TCCCGAGGGC 
AAGTGGAGCT GCCCCCACTG TGAGAAGGAG GGGATCCAGT GGGAGCCGAA GGACGACGAC 
GATGAAGAGG AGGAGGGCGG CTGCGAGGAG GAGGAGGACG ACCACATGGA GTTCTGCCGC 
GTGTGCAAGG ACGGGGGCGA GCTGCTCTGC TGCGACGCCT GCCCCTCCTC CTACCACCTG 
CATTGCCTCA ACCCGCCGCT GCCCGAGATC CCAAACGGTG AATGGCTCTG CCCGCGCTGT 
ACTTGCCCCC CACTGAAGGG CAAAGTCCAG CGGATTCTAC ACTGGAGGTG GACGGAGCCC 
CCTGCCCCCT TCATGGTGGG GCTGCCGGGG CCTGACGTGG AGCCCAGCCT CCCTCCACCT 
AAGCCCCTGG AGGGCATCCC TGAGAGAGAG TTCTTTGTCA AGTGGGCAGG GCTGTCCTAC 
TGGCATTGCT CCTGGGTGAA GGAGCTACAG CTGGAGCTGT ACCACACGGT GATGTATCGC 
AACTACCAAA GAAAGAACGA CATGGATGAG CCGCCCCCCT TTGACTACGG CTCTGGGGAT 
GAAGACGGCA AGAGCGAGAA GAGGAAGAAC AAGGACCCCC TCTATGCCAA GATGGAGGAG 
CGCTTCTACC GCTATGGCAT CAAGCCAGAG TGGATGATGA TTCACCGAAT CCTGAACCAT 
AGCTTTGACA AGAAGGGGGA TGTGCACTAC CTGATCAAGT GGAAAGACCT GCCCTACGAC 
CAGTGCACCT GGGAGATCGA TGACATCGAC ATCCCCTACT ACGACAACCT CAAGCAGGCC 
TACTGGGGCC ACAGGGAGCT GATGCTGGGA GAAGACACCA GGCTGCCCAA GAGGCTGCTC 
AAGAAGGGCA AGAAGCTGAG GGACGACAAG CAGGAGAAGC CGCCGGACAC GCCCATTGTG 
GACCCCACGG TCAAGTTCGA CAAGCAGCCA TGGTACATCG ACTCCACAGG CGGCACACTG 
CACCCGTACC AGCTGGAGGG CCTCAACTGG CTGCGCTTCT CTTGGGCCCA GGGCACTGAC 
ACCATCCTGG CCGATGAGAT GGGTCTGGGC AAGACGGTGC AGACCATCGT GTTCCTTTAC 
TCCCTCTACA AGGAGGGCCA CTCCAAAGGG CCCTACCTGG TTAGCGCGCC CCTCTCCACC 
ATCATCAACT GGGAACGCGA GTTTGAGATG TGGGCGCCCG ACTTCTACGT GGTCACCTAC 
ACGGGGGACA AGGAGAGCCG CTCGGTGATT CGGGAGAACG AGTTTTCCTT TGAGGACAAC 
GCCATTCGGA GTGGGAAGAA GGTATTCCGT ATGAAGAAAG AAGTGCAGAT CAAATTCCAC 
GTGCTGCTCA CCTCCTATGA GCTCATCACC ATTGACCAGG CCATCCTGGG CTCCATCGAG 
TGGGCCTGCC TGGTGGTAGA TGAGGCCCAC CGCCTCAAGA ACAACCAGTC CAAGTTTTTT 
AGGGTCTTAA ACAGCTACAA GATTGATTAC AAGCTGCTGC TGACAGGGAC CCCCCTTCAG 
AACAACCTGG AGGAGCTGTT CCATCTCCTC AACTTCCTGA CTCCAGAGAG GTTCAACAAC 
CTGGAGGGCT TCCTGGAGGA GTTTGCTGAC ATCTCCAAGG AAGACCAGAT CAAGAAGCTG 
CATGACCTGC TGGGGCCGCA CATGCTCAGG CGGCTCAAGG CTGACGTGTT CAAGAACATG 
CCGGCCAAGA CCGAGCTCAT TGTCCGGGTG GAGCTGAGCC AGATGCAGAA GAAGTACTAC 
AAGTTCATCC TCACACGGAA CTTTGAGGCA CTGAACTCCA AGGGGGGCGG GAACCAAGTA 
TCGCTGCTCA ACATCATGAT GGACCTGAAA AAGTGCTGCA ACCACCCCTA CCTCTTCCCT 
GTGGCTGCCG TGGAGGCCCC TGTCTTGCCC AATGGCTCCT ACGATGGAAG CTCCCTGGTC 
AAGTCTTCAG GGAAGCTCAT GCTGCTACAG AAGATGCTGA AGAAACTGCG GGATGAGGGG 
CACCGTGTGC TCATCTTCTC CCAGATGACC AAGATGCTGG ACCTCCTGGA GGACTTCCTG 
GAGTACGAAG GCTACAAGTA TGAGCGGATT GATGGTGGCA TCACCGGGGG CCTCCGGCAG 
GAGGCAATCG ACAGATTCAA TGCCCCCGGG GCCCAGCAGT TCTGCTTCCT CCTCTCAACC 
CGGGCAGGTG GTCTGGGCAT CAACCTGGCC ACGGCGGACA CTGTCATCAT CTACGACTCG 
GACTGGAACC CGCACAATGA CATCCAGGCC TTCAGCCGCG CCCACCGCAT CGGCCAGAAC 
AAGAAGGTGA TGATCTACCG CTTCGTGACT CGGGCCTCGG TGGAGGAGCG CATCACGCAG 
GTGGCCAAGC GCAAGATGAT GCTCACCCAC CTGGTGGTGC GGCCCGGCCT CGGCTCCAAG 
TCGGGGTCCA TGACCAAGCA GGAGCTGGAC GACATCCTCA AGTTCGGCAC GGAGGAACTC 
TTCAAGGACG ACGTGGAGGG CATGATGTCT CAGGGCCAGA GGCCGGTCAC ACCCATCCCT 
GATGTCCAGT CCTCCAAAGG GGGGAACTTG GCCGCCAGTG CAAAGAAGAA GCACGGTAGC 
ACCCCGCCAG GTGACAACAA GGACGTGGAG GACAGCAGTG TGATCCACTA TGACGATGCG 
GCCATCTCCA AGCTGCTGGA CCGGAACCAG GACGCTACAG ATGACACGGA GCTACAGAAC 
ATGAACGAGT ACCTGAGCTC CTTCAAGGTG GCGCAGTACG TGGTGCGCGA GGAGGACGGC 
GTGGAGGAGG TGGAGCGGGA AATCATCAAG CAGGAGGAGA ACGTGGACCC CGACTACTGG 
GAGAAGCTGC TGCGGCACCA CTATGAGCAG CAGCAGGAGG ACCTGGCCCG CAACCTGGGC 
AAGGGCAAGC GCATCCGCAA GCAGGTCAAC TACAACGATG CCTCCCAGGA GGACCAGGAG 
TGGCAGGATG AGCTCTCTGA TAACCAGTCA GAATATTCCA TTGGCTCTGA GGATGAGGAT 
GAGGACTTTG AAGAGAGGCC GGAAGGGCAG AGTGGACGAC GACAATCCCG GAGGCAGCTG 
AAGAGTGACA GGGACAAGCC CCTGCCCCCG CTTCTCGCCC GAGTTGGTGG CAACATCGAG 
GTGCTGGGCT TCAATGCCCG ACAGCGGAAG GCCTTTCTGA ACGCCATCAT GCGCTGGGGC 
ATGCCCCCGC AGGACGCCTT CAACTCCCAC TGGCTGGTGC GGGACCTTCG AGGGAAGAGC 
GAGAAGGAGT TTAGAGCCTA TGTGTCCCTC TTCATGCGGC ACCTGTGTGA GCCGGGGGCG 
GATGGTGCAG AGACCTTCGC AGACGGCGTG CCCCGGGAGG GCCTCTCCAG GCAGCACGTG 
CTGACCCGCA TCGGGGTCAT GTCACTAGTT AGGAAGAAGG TTCAGGAGTT TGAGCATGTC 
AACGGGAAGT ACAGCACCCC AGACTTGATC CCTGAGGGGC CCGAGGGGAA GAAGTCGGGC 
GAGGTGATCT CCTCGGACCC CAACACACCA GTGCCCGCCA GCCCTGCCCA CCTCCTGCCA 
GCCCCGCTGG GCCTGCCAGA CAAAATGGAA GCCCAGCTGG GCTACATGGA TGAGAAAGAC 
CCCGGGGCAC AGAAGCCAAG GCAGCCCCTG GAAGTCCAGG CCCTTCCAGC CGCCTTGGAT 
AGAGTGGAGA GTGAGGACAA GCACGAGAGC CCAGCCAGCA AGGAGAGAGC CCGAGAGGAG 
CGGCCAGAGG AGACGGAGAA GGCCCCGCCC TCCCCGGAGC AGCTGCCGAG AGAGGAGGTG 
CTTCCTGAGA AGGAGAAGAT CCTGGACAAG CTGGAGCTGA GCTTGATCCA CAGCAGAGGG 
GACAGTTCCG AACTCAGGCC AGATGACACC AAGGCTGAGG AGAAGGAGCC CATTGAAACA 
CAGCAAAATG GTGACAAAGA GGAAGATGAC GAGGGGAAGA AGGAGGACAA GAAGGGGAAA 
TTCAAGTTCA TGTTCAACAT CGCGGACGGG GGCTTCACGG AGTTGCACAC GCTGTGGCAG 
AACGAGGAGC GGGCTGCTGT ATCCTCTGGG AAAATCTACG ACATCTGGCA CCGGCGCCAT 
GACTACTGGC TGCTGGCGGG CATCGTGACG CACGGCTACG CCCGCTGGCA GGACATCCAG 
AATGACCCAC GGTACATGAT CCTCAACGAG CCCTTCAAGT CTGAGGTCCA CAAGGGCAAC 
TACCTGGAGA TGAAGAACAA GTTCCTGGCC CGCAGGTTTA AGCTGCTGGA GCAGGCGTTG 
GTCATTGAGG AGCAGCTCCG GAGGGCCGCG TACCTGAACA TGACGCAGGA CCCCAACCAC 
CCCGCCATGG CCCTCAACGC CCGCCTGGCT GAAGTGGAGT GCCTCGCCGA GAGCCACCAG 
CACCTGTCCA AGGAGTCCCT TGCTGGGAAC AAGCCTGCCA ATGCCGTCCT GCACAAGGTC 
CTGAACCAGC TGGAGGAGCT GCTGAGCGAC ATGAAGGCCG ACGTGACCCG GCTGCCATCC 
ATGCTGTCCC GCATCCCCCC GGTGGCCGCC CGGCTGCAGA TGTCGGAGCG CAGCATCCTG 
AGCCGCCTGA CCAACCGCGC CGGGGACCCC ACCATCCAGC AGGGCGCTTT CGGCTCCTCC 
CAGATGTACA GCAACAACTT TGGGCCCAAC TTCCGGGGCC CTGGACCGGG AGGGATTGTC 
AACTACAACC AGATGCCCCT GGGGCCCTAT GTGACCGATA TCTAG