GJD4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GJD4
Entrez Gene ID	219770
Full Name	gap junction protein delta 4
Synonyms	CX40.1
General protein information	Preferred Names gap junction protein delta 4 Names gap junction delta-4 protein connexin-40.1 connexin40.1 gap junction protein, delta 4, 40.1kDa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10p11.21
Summary	Connexins, such as GJD4, are involved in the formation of gap junctions, intercellular conduits that directly connect the cytoplasms of contacting cells. Each gap junction channel is formed by docking of 2 hemichannels, each of which contains 6 connexin subunits (Sohl et al., 2003 [PubMed 12881038]).[supplied by OMIM, Mar 2008].
Disorder MIM:	611922

mRNA and Protein(s)

mRNA	Protein	Name
NM_153368.3	NP_699199.2	gap junction delta-4 protein
NM_153368.2	NP_699199.2	gap junction delta-4 protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	GJD4	NP_699199.2
Pan troglodytes (chimpanzee)	GJD4	XP_001150863.1
Bos taurus (cattle)	GJD4	XP_002692120.1
Canis lupus familiaris (dog)	GJD4	XP_848529.2
Gallus gallus (chicken)	GJD4	XP_418567.2
	GJD4	XP_001094309.1
Mus musculus (house mouse)	Gjd4	NP_694726.2
Rattus norvegicus (Norway rat)	Gjd4	NP_001093957.1

Gene Ontology
Bibliography

Related articles in PubMed

The absence of dysferlin induces the expression of functional connexin-based hemichannels in human myotubes.
Cea LA, Bevilacqua JA, Arriagada C, C?rdenas AM, Bigot A, Mouly V, S?ez JC, Caviedes P
BMC cell biology17 Suppl 115(2016 May)

Connexin40 regulates platelet function.
Vaiyapuri S, Moraes LA, Sage T, Ali MS, Lewis KR, Mahaut-Smith MP, Oviedo-Orta E, Simon AM, Gibbins JM
Nature communications42564(2013)

Inducible coexpression of connexin37 or connexin40 with connexin43 selectively affects intercellular molecular transfer.
Gemel J, Nelson TK, Burt JM, Beyer EC
The Journal of membrane biology245(5-6)231-41(2012 Jun)

Expression profiles of the novel human connexin genes hCx30.2, hCx40.1, and hCx62 differ from their putative mouse orthologues.
S?hl G, Nielsen PA, Eiberger J, Willecke K
Cell communication & adhesion10(1)27-36(2003 Jan-Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Human deltoid muscle biopsies of 5 Chilean dysferlinopathy patients exhibited the presence of muscular connexins (Cx40.1, Cx43 and Cx45).
Title: The absence of dysferlin induces the expression of functional connexin-based hemichannels in human myotubes.

Connexin40 regulates platelet function.
Title: Connexin40 regulates platelet function.

The data show that induced expression of either Cx37 or Cx40 in Cx43-expressing cells can selectively alter the intercellular exchange of some molecules without affecting the transfer of others.
Title: Inducible coexpression of connexin37 or connexin40 with connexin43 selectively affects intercellular molecular transfer.

expression patterns differ from mouse orthologs [Cx30.2; Cx40.1]
Title: Expression profiles of the novel human connexin genes hCx30.2, hCx40.1, and hCx62 differ from their putative mouse orthologues.

The following GJD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GJD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19840	NM_153368.3 Latest version!	Homo sapiens gap junction protein delta 4 (GJD4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
View Old Accession Versions >>
OHu19840	NM_153368.2	Homo sapiens gap junction protein delta 4 (GJD4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GJD4 ( NM_153368.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_699199.2 Homo sapiens gap junction protein delta 4 (GJD4), mRNA.

Price & Availability↑

CloneID	OHu19840
Clone ID Related Accession (Same CDS sequence)	NM_153368.3 , NM_153368.2
Accession Version	NM_153368.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	gap junction delta-4 protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL121749.14, AK074504.1 and AJ414564.1. On May 17, 2019 this sequence version replaced NM_153368.2. ##Evidence-Data-START## Transcript exon combination :: AK074504.1, AJ414564.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000321660.2/ ENSP00000315070.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGGAAGGCG TGGACTTGCT AGGGTTTCTC ATCATCACAT TAAACTGCAA CGTGACCATG 
GTGGGAAAGC TCTGGTTCGT CCTCACGATG CTGCTGCGGA TGCTGGTGAT TGTCTTGGCG 
GGGCGACCCG TCTACCAGGA CGAGCAGGAG AGGTTTGTCT GCAACACGCT GCAGCCGGGA 
TGCGCCAATG TTTGCTACGA CGTCTTCTCC CCCGTGTCTC ACCTGCGGTT CTGGCTGATC 
CAGGGCGTGT GCGTCCTCCT CCCCTCCGCC GTCTTCAGCG TCTATGTCCT GCACCGAGGA 
GCCACGCTCG CCGCGCTGGG CCCCCGCCGC TGCCCCGACC CCCGGGAGCC GGCCTCCGGG 
CAGAGACGCT GCCCGCGGCC ATTCGGGGAG CGCGGCGGCC TCCAGGTGCC CGACTTTTCG 
GCCGGCTACA TCATCCACCT CCTCCTCCGG ACCCTGCTGG AGGCAGCCTT CGGGGCCTTG 
CACTACTTTC TCTTTGGATT CCTGGCCCCG AAGAAGTTCC CTTGCACGCG CCCTCCGTGC 
ACGGGCGTGG TGGACTGCTA CGTGTCGCGG CCCACAGAGA AGTCCCTGCT GATGCTGTTC 
CTCTGGGCGG TCAGCGCGCT GTCTTTTCTG CTGGGCCTCG CCGACCTGGT CTGCAGCCTG 
CGGCGGCGGA TGCGCAGGAG GCCGGGACCC CCCACAAGCC CCTCCATCCG GAAGCAGAGC 
GGAGCCTCAG GCCACGCGGA GGGACGCCGG ACTGACGAGG AGGGTGGGCG GGAGGAAGAG 
GGGGCACCGG CGCCCCCGGG TGCACGCGCC GGAGGGGAGG GGGCTGGCAG CCCCAGGCGT 
ACATCCAGGG TGTCAGGGCA CACGAAGATT CCGGATGAGG ATGAGAGTGA GGTGACATCC 
TCCGCCAGCG AAAAGCTGGG CAGACAGCCC CGGGGCAGGC CCCACCGAGA GGCCGCCCAG 
GACCCCAGGG GCTCAGGATC CGAGGAGCAG CCCTCAGCAG CCCCCAGCCG CCTGGCCGCG 
CCCCCTTCCT GCAGCAGCCT GCAGCCCCCT GACCCGCCTG CCAGCTCCAG TGGTGCTCCC 
CACCTGAGAG CCAGGAAGTC TGAGTGGGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_699199.2
CDS	228..1340
Translation	MEGVDLLGFLIITLNCNVTMVGKLWFVLTMLLRMLVIVLAGRPVYQDEQERFVCNTLQPGCANVCYDVFSPVSHLRFWLIQGVCVLLPSAVFSVYVLHRGATLAALGPRRCPDPREPASGQRRCPRPFGERGGLQVPDFSAGYIIHLLLRTLLEAAFGALHYFLFGFLAPKKFPCTRPPCTGVVDCYVSRPTEKSLLMLFLWAVSALSFLLGLADLVCSLRRRMRRRPGPPTSPSIRKQSGASGHAEGRRTDEEGGREEEGAPAPPGARAGGEGAGSPRRTSRVSGHTKIPDEDESEVTSSASEKLGRQPRGRPHREAAQDPRGSGSEEQPSAAPSRLAAPPSCSSLQPPDPPASSSGAPHLRARKSEWV

Target ORF information:

RefSeq Version	NM_153368.3
Organism	Homo sapiens(human)
Definition	Homo sapiens gap junction protein delta 4 (GJD4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153368.3

ORF Insert Sequence:

ATGGAAGGCG TGGACTTGCT AGGGTTTCTC ATCATCACAT TAAACTGCAA CGTGACCATG 
GTGGGAAAGC TCTGGTTCGT CCTCACGATG CTGCTGCGGA TGCTGGTGAT TGTCTTGGCG 
GGGCGACCCG TCTACCAGGA CGAGCAGGAG AGGTTTGTCT GCAACACGCT GCAGCCGGGA 
TGCGCCAATG TTTGCTACGA CGTCTTCTCC CCCGTGTCTC ACCTGCGGTT CTGGCTGATC 
CAGGGCGTGT GCGTCCTCCT CCCCTCCGCC GTCTTCAGCG TCTATGTCCT GCACCGAGGA 
GCCACGCTCG CCGCGCTGGG CCCCCGCCGC TGCCCCGACC CCCGGGAGCC GGCCTCCGGG 
CAGAGACGCT GCCCGCGGCC ATTCGGGGAG CGCGGCGGCC TCCAGGTGCC CGACTTTTCG 
GCCGGCTACA TCATCCACCT CCTCCTCCGG ACCCTGCTGG AGGCAGCCTT CGGGGCCTTG 
CACTACTTTC TCTTTGGATT CCTGGCCCCG AAGAAGTTCC CTTGCACGCG CCCTCCGTGC 
ACGGGCGTGG TGGACTGCTA CGTGTCGCGG CCCACAGAGA AGTCCCTGCT GATGCTGTTC 
CTCTGGGCGG TCAGCGCGCT GTCTTTTCTG CTGGGCCTCG CCGACCTGGT CTGCAGCCTG 
CGGCGGCGGA TGCGCAGGAG GCCGGGACCC CCCACAAGCC CCTCCATCCG GAAGCAGAGC 
GGAGCCTCAG GCCACGCGGA GGGACGCCGG ACTGACGAGG AGGGTGGGCG GGAGGAAGAG 
GGGGCACCGG CGCCCCCGGG TGCACGCGCC GGAGGGGAGG GGGCTGGCAG CCCCAGGCGT 
ACATCCAGGG TGTCAGGGCA CACGAAGATT CCGGATGAGG ATGAGAGTGA GGTGACATCC 
TCCGCCAGCG AAAAGCTGGG CAGACAGCCC CGGGGCAGGC CCCACCGAGA GGCCGCCCAG 
GACCCCAGGG GCTCAGGATC CGAGGAGCAG CCCTCAGCAG CCCCCAGCCG CCTGGCCGCG 
CCCCCTTCCT GCAGCAGCCT GCAGCCCCCT GACCCGCCTG CCAGCTCCAG TGGTGCTCCC 
CACCTGAGAG CCAGGAAGTC TGAGTGGGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GJD4 ( NM_153368.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_699199.2 Homo sapiens gap junction protein delta 4 (GJD4), mRNA.

Price & Availability↑

CloneID	OHu19840
Clone ID Related Accession (Same CDS sequence)	NM_153368.3 , NM_153368.2
Accession Version	NM_153368.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	gap junction delta-4 protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK074504.1 and AJ414564.1. On Apr 25, 2007 this sequence version replaced NM_153368.1. ##Evidence-Data-START## Transcript exon combination :: AK074504.1, AJ414564.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGGCG TGGACTTGCT AGGGTTTCTC ATCATCACAT TAAACTGCAA CGTGACCATG 
GTGGGAAAGC TCTGGTTCGT CCTCACGATG CTGCTGCGGA TGCTGGTGAT TGTCTTGGCG 
GGGCGACCCG TCTACCAGGA CGAGCAGGAG AGGTTTGTCT GCAACACGCT GCAGCCGGGA 
TGCGCCAATG TTTGCTACGA CGTCTTCTCC CCCGTGTCTC ACCTGCGGTT CTGGCTGATC 
CAGGGCGTGT GCGTCCTCCT CCCCTCCGCC GTCTTCAGCG TCTATGTCCT GCACCGAGGA 
GCCACGCTCG CCGCGCTGGG CCCCCGCCGC TGCCCCGACC CCCGGGAGCC GGCCTCCGGG 
CAGAGACGCT GCCCGCGGCC ATTCGGGGAG CGCGGCGGCC TCCAGGTGCC CGACTTTTCG 
GCCGGCTACA TCATCCACCT CCTCCTCCGG ACCCTGCTGG AGGCAGCCTT CGGGGCCTTG 
CACTACTTTC TCTTTGGATT CCTGGCCCCG AAGAAGTTCC CTTGCACGCG CCCTCCGTGC 
ACGGGCGTGG TGGACTGCTA CGTGTCGCGG CCCACAGAGA AGTCCCTGCT GATGCTGTTC 
CTCTGGGCGG TCAGCGCGCT GTCTTTTCTG CTGGGCCTCG CCGACCTGGT CTGCAGCCTG 
CGGCGGCGGA TGCGCAGGAG GCCGGGACCC CCCACAAGCC CCTCCATCCG GAAGCAGAGC 
GGAGCCTCAG GCCACGCGGA GGGACGCCGG ACTGACGAGG AGGGTGGGCG GGAGGAAGAG 
GGGGCACCGG CGCCCCCGGG TGCACGCGCC GGAGGGGAGG GGGCTGGCAG CCCCAGGCGT 
ACATCCAGGG TGTCAGGGCA CACGAAGATT CCGGATGAGG ATGAGAGTGA GGTGACATCC 
TCCGCCAGCG AAAAGCTGGG CAGACAGCCC CGGGGCAGGC CCCACCGAGA GGCCGCCCAG 
GACCCCAGGG GCTCAGGATC CGAGGAGCAG CCCTCAGCAG CCCCCAGCCG CCTGGCCGCG 
CCCCCTTCCT GCAGCAGCCT GCAGCCCCCT GACCCGCCTG CCAGCTCCAG TGGTGCTCCC 
CACCTGAGAG CCAGGAAGTC TGAGTGGGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_699199.2
CDS	159..1271
Translation	MEGVDLLGFLIITLNCNVTMVGKLWFVLTMLLRMLVIVLAGRPVYQDEQERFVCNTLQPGCANVCYDVFSPVSHLRFWLIQGVCVLLPSAVFSVYVLHRGATLAALGPRRCPDPREPASGQRRCPRPFGERGGLQVPDFSAGYIIHLLLRTLLEAAFGALHYFLFGFLAPKKFPCTRPPCTGVVDCYVSRPTEKSLLMLFLWAVSALSFLLGLADLVCSLRRRMRRRPGPPTSPSIRKQSGASGHAEGRRTDEEGGREEEGAPAPPGARAGGEGAGSPRRTSRVSGHTKIPDEDESEVTSSASEKLGRQPRGRPHREAAQDPRGSGSEEQPSAAPSRLAAPPSCSSLQPPDPPASSSGAPHLRARKSEWV

Target ORF information:

RefSeq Version	NM_153368.2
Organism	Homo sapiens(human)
Definition	Homo sapiens gap junction protein delta 4 (GJD4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153368.2

ORF Insert Sequence:

ATGGAAGGCG TGGACTTGCT AGGGTTTCTC ATCATCACAT TAAACTGCAA CGTGACCATG 
GTGGGAAAGC TCTGGTTCGT CCTCACGATG CTGCTGCGGA TGCTGGTGAT TGTCTTGGCG 
GGGCGACCCG TCTACCAGGA CGAGCAGGAG AGGTTTGTCT GCAACACGCT GCAGCCGGGA 
TGCGCCAATG TTTGCTACGA CGTCTTCTCC CCCGTGTCTC ACCTGCGGTT CTGGCTGATC 
CAGGGCGTGT GCGTCCTCCT CCCCTCCGCC GTCTTCAGCG TCTATGTCCT GCACCGAGGA 
GCCACGCTCG CCGCGCTGGG CCCCCGCCGC TGCCCCGACC CCCGGGAGCC GGCCTCCGGG 
CAGAGACGCT GCCCGCGGCC ATTCGGGGAG CGCGGCGGCC TCCAGGTGCC CGACTTTTCG 
GCCGGCTACA TCATCCACCT CCTCCTCCGG ACCCTGCTGG AGGCAGCCTT CGGGGCCTTG 
CACTACTTTC TCTTTGGATT CCTGGCCCCG AAGAAGTTCC CTTGCACGCG CCCTCCGTGC 
ACGGGCGTGG TGGACTGCTA CGTGTCGCGG CCCACAGAGA AGTCCCTGCT GATGCTGTTC 
CTCTGGGCGG TCAGCGCGCT GTCTTTTCTG CTGGGCCTCG CCGACCTGGT CTGCAGCCTG 
CGGCGGCGGA TGCGCAGGAG GCCGGGACCC CCCACAAGCC CCTCCATCCG GAAGCAGAGC 
GGAGCCTCAG GCCACGCGGA GGGACGCCGG ACTGACGAGG AGGGTGGGCG GGAGGAAGAG 
GGGGCACCGG CGCCCCCGGG TGCACGCGCC GGAGGGGAGG GGGCTGGCAG CCCCAGGCGT 
ACATCCAGGG TGTCAGGGCA CACGAAGATT CCGGATGAGG ATGAGAGTGA GGTGACATCC 
TCCGCCAGCG AAAAGCTGGG CAGACAGCCC CGGGGCAGGC CCCACCGAGA GGCCGCCCAG 
GACCCCAGGG GCTCAGGATC CGAGGAGCAG CCCTCAGCAG CCCCCAGCCG CCTGGCCGCG 
CCCCCTTCCT GCAGCAGCCT GCAGCCCCCT GACCCGCCTG CCAGCTCCAG TGGTGCTCCC 
CACCTGAGAG CCAGGAAGTC TGAGTGGGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The absence of dysferlin induces the expression of functional connexin-based hemichannels in human myotubes.
BMC cell biology17 Suppl 115(2016 May)
Cea LA,Bevilacqua JA,Arriagada C,C?rdenas AM,Bigot A,Mouly V,S?ez JC,Caviedes P

Connexin40 regulates platelet function.
Nature communications42564(2013)
Vaiyapuri S,Moraes LA,Sage T,Ali MS,Lewis KR,Mahaut-Smith MP,Oviedo-Orta E,Simon AM,Gibbins JM

Inducible coexpression of connexin37 or connexin40 with connexin43 selectively affects intercellular molecular transfer.
The Journal of membrane biology245(5-6)231-41(2012 Jun)
Gemel J,Nelson TK,Burt JM,Beyer EC

Expression profiles of the novel human connexin genes hCx30.2, hCx40.1, and hCx62 differ from their putative mouse orthologues.
Cell communication & adhesion10(1)27-36(2003 Jan-Feb)
S?hl G,Nielsen PA,Eiberger J,Willecke K