Gjd4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gjd4
Entrez Gene ID	225152
Full Name	gap junction protein, delta 4
Synonyms	9430022F06Rik,Cx39
General protein information	Preferred Names gap junction protein, delta 4 Names gap junction delta-4 protein connexin-39 connexin39
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 A1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006525809.2	XP_006525872.1	gap junction delta-4 protein isoform X1
XM_006525809.3	XP_006525872.1	gap junction delta-4 protein isoform X1
NM_153086.5	NP_694726.2	gap junction delta-4 protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	GJD4	NP_699199.2
Pan troglodytes (chimpanzee)	GJD4	XP_001150863.1
Bos taurus (cattle)	GJD4	XP_002692120.1
Canis lupus familiaris (dog)	GJD4	XP_848529.2
Gallus gallus (chicken)	GJD4	XP_418567.2
	GJD4	XP_001094309.1
Mus musculus (house mouse)	Gjd4	NP_694726.2
Rattus norvegicus (Norway rat)	Gjd4	NP_001093957.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Connexin39 deficient mice display accelerated myogenesis and regeneration of skeletal muscle.
von Maltzahn J, Wulf V, Matern G, Willecke K
Experimental cell research317(8)1169-78(2011 May)

Spatiotemporal expression of connexin 39 and -43 during myoblast differentiation in cultured cells and in the mouse embryo.
von Maltzahn J, Wulf V, Willecke K
Cell communication & adhesion13(1-2)55-60(2006 Jan-Apr)

Cx37 and Cx43 localize to zona pellucida in mouse ovarian follicles.
Simon AM, Chen H, Jackson CL
Cell communication & adhesion13(1-2)61-77(2006 Jan-Apr)

The novel mouse connexin39 gene is expressed in developing striated muscle fibers.
von Maltzahn J, Euwens C, Willecke K, S?hl G
Journal of cell science117(Pt 22)5381-92(2004 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that myogenesis in Cx39 KO mice is accelerated by increased myogenin expression on ED13.5 and 16.5 and increased Cx43 in developing skeletal muscle, and suggest that Cx43 may compensate for loss of Cx39 during myogenesis and regeneration.
Title: Connexin39 deficient mice display accelerated myogenesis and regeneration of skeletal muscle.

Upon differentiation into myotubes, Cx39 expression increased in mouse embryo.
Title: Spatiotemporal expression of connexin 39 and -43 during myoblast differentiation in cultured cells and in the mouse embryo.

Immunofluorescence staining revealed abundant Cx37 and Cx43 puncta within the zona pellucida of both preantral and antral follicles
Title: Cx37 and Cx43 localize to zona pellucida in mouse ovarian follicles.

connexin39 gene is expressed in developing striated muscle fibers
Title: The novel mouse connexin39 gene is expressed in developing striated muscle fibers.

The following Gjd4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gjd4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02248	NM_153086.5 Latest version!	Mus musculus gap junction protein, delta 4 (Gjd4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu54495	XM_006525809.3 Latest version!	Mus musculus gap junction protein, delta 4 (Gjd4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu54495	XM_006525809.2	Mus musculus gap junction protein, delta 4 (Gjd4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gjd4 ( NM_153086.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_694726.2 Mus musculus gap junction protein, delta 4 (Gjd4), mRNA.

Price & Availability↑

CloneID	OMu02248
Clone ID Related Accession (Same CDS sequence)	NM_153086.5
Accession Version	NM_153086.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	gap junction delta-4 protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC108777.7. On May 1, 2007 this sequence version replaced NM_153086.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK034663.1, AK053016.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN01164134 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAAGT TGAACTTGTT GGGATTCCTC ATCATCACCT TAAACTGTAA CGTGACCATC 
ATGGGCATGA TCTGGCTGAT CGTGGAGGTC TTGCTGAGGA TGCTAGTGGT GGTCTTGGCA 
GGGTCACCTA TCTATGAGGA TGAACAAGAG AGGTTTATTT GCAACACACT GCAACCAGGA 
TGTGCCAACG TTTGCTACGA CCTCTTTTCC CCAGTGTCAC CGCTGCGATT CTGGCTAGTG 
CAGAGCCTGG CCTTGCTTCT GCCTTCGGTG GTCTTTGGCA CTTACACCCT ACACCGCGGT 
GCGAAGCTGG CTGCAGTGGG GGGAGCCTGC AGGCCCCAGG TGCCCGACCT GTCTACTGCC 
TACCTGGTGC ACCTACTGCT GCGCATGCTG CTGGAGGCCG GGCTGGCCTT CCTGCACTAC 
TTTCTCTTTG GCTTTTCTGT GCCCGCCCGC GTGTCTTGCT CGCATGTACC CTGCTCAGGG 
GCTGTGGACT GCTACGTGTC GCGGCCCACG GAGAAGTCAC TCCTGATACT ATTCTTTTGG 
GCAGTGAGTG CGCTATCCTT CCTGCTCAGC TTGGCTGACC TGCTTTGGAT CCTGCCGAGG 
AGAAAGACAC TGAGGACCAC GCAGTGGGTG AATGGAGAGG CTAGACCAGT CTGTGAAGTA 
CCTGCACCTC CCCCTTGCCT CTTACAAAAC CCCCAGGGCT ATCTTAGCCA AGGTCAGGTG 
GACCAAGAGG ACAGACAGGA GGAACAAGTT GTGCCTGAGT TCCCCTGCAT GTGGACAGCA 
GGGCAGAGTG ACAACAGCAA TGTTGGTCAG GCCTGTGTGT CGGGGCTGCT GGAACATTCA 
GACCAAGATG CTAGTGAGGC CACTTCCTCA GCTGGTGACA GGCTAACAGT GGCTCACACA 
GCACATGAGC TCAGATTCCA CAGAGAGACT TCACTGGACC TGGGGGGCAA AAACACCCAG 
GCAGATGAAC TCTCCTTGGC TACCCAGAGC CACCTGGCCA GACACAGTTC AGCCAGCAAG 
CCTCAAGCTC CATGCCGGCT GACCACCTCA GGCAGTGCTC CCCATTTGAG AACCAAAAAA 
TCTGAGTGGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_694726.2
CDS	192..1286
Translation	MEKLNLLGFLIITLNCNVTIMGMIWLIVEVLLRMLVVVLAGSPIYEDEQERFICNTLQPGCANVCYDLFSPVSPLRFWLVQSLALLLPSVVFGTYTLHRGAKLAAVGGACRPQVPDLSTAYLVHLLLRMLLEAGLAFLHYFLFGFSVPARVSCSHVPCSGAVDCYVSRPTEKSLLILFFWAVSALSFLLSLADLLWILPRRKTLRTTQWVNGEARPVCEVPAPPPCLLQNPQGYLSQGQVDQEDRQEEQVVPEFPCMWTAGQSDNSNVGQACVSGLLEHSDQDASEATSSAGDRLTVAHTAHELRFHRETSLDLGGKNTQADELSLATQSHLARHSSASKPQAPCRLTTSGSAPHLRTKKSEWV

Target ORF information:

RefSeq Version	NM_153086.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus gap junction protein, delta 4 (Gjd4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153086.5

ORF Insert Sequence:

ATGGAGAAGT TGAACTTGTT GGGATTCCTC ATCATCACCT TAAACTGTAA CGTGACCATC 
ATGGGCATGA TCTGGCTGAT CGTGGAGGTC TTGCTGAGGA TGCTAGTGGT GGTCTTGGCA 
GGGTCACCTA TCTATGAGGA TGAACAAGAG AGGTTTATTT GCAACACACT GCAACCAGGA 
TGTGCCAACG TTTGCTACGA CCTCTTTTCC CCAGTGTCAC CGCTGCGATT CTGGCTAGTG 
CAGAGCCTGG CCTTGCTTCT GCCTTCGGTG GTCTTTGGCA CTTACACCCT ACACCGCGGT 
GCGAAGCTGG CTGCAGTGGG GGGAGCCTGC AGGCCCCAGG TGCCCGACCT GTCTACTGCC 
TACCTGGTGC ACCTACTGCT GCGCATGCTG CTGGAGGCCG GGCTGGCCTT CCTGCACTAC 
TTTCTCTTTG GCTTTTCTGT GCCCGCCCGC GTGTCTTGCT CGCATGTACC CTGCTCAGGG 
GCTGTGGACT GCTACGTGTC GCGGCCCACG GAGAAGTCAC TCCTGATACT ATTCTTTTGG 
GCAGTGAGTG CGCTATCCTT CCTGCTCAGC TTGGCTGACC TGCTTTGGAT CCTGCCGAGG 
AGAAAGACAC TGAGGACCAC GCAGTGGGTG AATGGAGAGG CTAGACCAGT CTGTGAAGTA 
CCTGCACCTC CCCCTTGCCT CTTACAAAAC CCCCAGGGCT ATCTTAGCCA AGGTCAGGTG 
GACCAAGAGG ACAGACAGGA GGAACAAGTT GTGCCTGAGT TCCCCTGCAT GTGGACAGCA 
GGGCAGAGTG ACAACAGCAA TGTTGGTCAG GCCTGTGTGT CGGGGCTGCT GGAACATTCA 
GACCAAGATG CTAGTGAGGC CACTTCCTCA GCTGGTGACA GGCTAACAGT GGCTCACACA 
GCACATGAGC TCAGATTCCA CAGAGAGACT TCACTGGACC TGGGGGGCAA AAACACCCAG 
GCAGATGAAC TCTCCTTGGC TACCCAGAGC CACCTGGCCA GACACAGTTC AGCCAGCAAG 
CCTCAAGCTC CATGCCGGCT GACCACCTCA GGCAGTGCTC CCCATTTGAG AACCAAAAAA 
TCTGAGTGGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gjd4 ( XM_006525809.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525872.1 Mus musculus gap junction protein, delta 4 (Gjd4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu54495
Clone ID Related Accession (Same CDS sequence)	XM_006525809.2 , XM_006525809.3
Accession Version	XM_006525809.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	gap junction delta-4 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006525809.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGGC TGATCGTGGA GGTCTTGCTG AGGATGCTAG TGGTGGTCTT GGCAGGGTCA 
CCTATCTATG AGGATGAACA AGAGAGGTTT ATTTGCAACA CACTGCAACC AGGATGTGCC 
AACGTTTGCT ACGACCTCTT TTCCCCAGTG TCACCGCTGC GATTCTGGCT AGTGCAGAGC 
CTGGCCTTGC TTCTGCCTTC GGTGGTCTTT GGCACTTACA CCCTACACCG CGGTGCGAAG 
CTGGCTGCAG TGGGGGGAGC CTGCAGGCCC CAGGTGCCCG ACCTGTCTAC TGCCTACCTG 
GTGCACCTAC TGCTGCGCAT GCTGCTGGAG GCCGGGCTGG CCTTCCTGCA CTACTTTCTC 
TTTGGCTTTT CTGTGCCCGC CCGCGTGTCT TGCTCGCATG TACCCTGCTC AGGGGCTGTG 
GACTGCTACG TGTCGCGGCC CACGGAGAAG TCACTCCTGA TACTATTCTT TTGGGCAGTG 
AGTGCGCTAT CCTTCCTGCT CAGCTTGGCT GACCTGCTTT GGATCCTGCC GAGGAGAAAG 
ACACTGAGGA CCACGCAGTG GGTGAATGGA GAGGCTAGAC CAGTCTGTGA AGTACCTGCA 
CCTCCCCCTT GCCTCTTACA AAACCCCCAG GGCTATCTTA GCCAAGGTCA GGTGGACCAA 
GAGGACAGAC AGGAGGAACA AGTTGTGCCT GAGTTCCCCT GCATGTGGAC AGCAGGGCAG 
AGTGACAACA GCAATGTTGG TCAGGCCTGT GTGTCGGGGC TGCTGGAACA TTCAGACCAA 
GATGCTAGTG AGGCCACTTC CTCAGCTGGT GACAGGCTAA CAGTGGCTCA CACAGCACAT 
GAGCTCAGAT TCCACAGAGA GACTTCACTG GACCTGGGGG GCAAAAACAC CCAGGCAGAT 
GAACTCTCCT TGGCTACCCA GAGCCACCTG GCCAGACACA GTTCAGCCAG CAAGCCTCAA 
GCTCCATGCC GGCTGACCAC CTCAGGCAGT GCTCCCCATT TGAGAACCAA AAAATCTGAG 
TGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525872.1
CDS	85..1113
Translation	MIWLIVEVLLRMLVVVLAGSPIYEDEQERFICNTLQPGCANVCYDLFSPVSPLRFWLVQSLALLLPSVVFGTYTLHRGAKLAAVGGACRPQVPDLSTAYLVHLLLRMLLEAGLAFLHYFLFGFSVPARVSCSHVPCSGAVDCYVSRPTEKSLLILFFWAVSALSFLLSLADLLWILPRRKTLRTTQWVNGEARPVCEVPAPPPCLLQNPQGYLSQGQVDQEDRQEEQVVPEFPCMWTAGQSDNSNVGQACVSGLLEHSDQDASEATSSAGDRLTVAHTAHELRFHRETSLDLGGKNTQADELSLATQSHLARHSSASKPQAPCRLTTSGSAPHLRTKKSEWV

Target ORF information:

RefSeq Version	XM_006525809.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus gap junction protein, delta 4 (Gjd4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525809.2

ORF Insert Sequence:

ATGATCTGGC TGATCGTGGA GGTCTTGCTG AGGATGCTAG TGGTGGTCTT GGCAGGGTCA 
CCTATCTATG AGGATGAACA AGAGAGGTTT ATTTGCAACA CACTGCAACC AGGATGTGCC 
AACGTTTGCT ACGACCTCTT TTCCCCAGTG TCACCGCTGC GATTCTGGCT AGTGCAGAGC 
CTGGCCTTGC TTCTGCCTTC GGTGGTCTTT GGCACTTACA CCCTACACCG CGGTGCGAAG 
CTGGCTGCAG TGGGGGGAGC CTGCAGGCCC CAGGTGCCCG ACCTGTCTAC TGCCTACCTG 
GTGCACCTAC TGCTGCGCAT GCTGCTGGAG GCCGGGCTGG CCTTCCTGCA CTACTTTCTC 
TTTGGCTTTT CTGTGCCCGC CCGCGTGTCT TGCTCGCATG TACCCTGCTC AGGGGCTGTG 
GACTGCTACG TGTCGCGGCC CACGGAGAAG TCACTCCTGA TACTATTCTT TTGGGCAGTG 
AGTGCGCTAT CCTTCCTGCT CAGCTTGGCT GACCTGCTTT GGATCCTGCC GAGGAGAAAG 
ACACTGAGGA CCACGCAGTG GGTGAATGGA GAGGCTAGAC CAGTCTGTGA AGTACCTGCA 
CCTCCCCCTT GCCTCTTACA AAACCCCCAG GGCTATCTTA GCCAAGGTCA GGTGGACCAA 
GAGGACAGAC AGGAGGAACA AGTTGTGCCT GAGTTCCCCT GCATGTGGAC AGCAGGGCAG 
AGTGACAACA GCAATGTTGG TCAGGCCTGT GTGTCGGGGC TGCTGGAACA TTCAGACCAA 
GATGCTAGTG AGGCCACTTC CTCAGCTGGT GACAGGCTAA CAGTGGCTCA CACAGCACAT 
GAGCTCAGAT TCCACAGAGA GACTTCACTG GACCTGGGGG GCAAAAACAC CCAGGCAGAT 
GAACTCTCCT TGGCTACCCA GAGCCACCTG GCCAGACACA GTTCAGCCAG CAAGCCTCAA 
GCTCCATGCC GGCTGACCAC CTCAGGCAGT GCTCCCCATT TGAGAACCAA AAAATCTGAG 
TGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gjd4 ( XM_006525809.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525872.1 Mus musculus gap junction protein, delta 4 (Gjd4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu54495
Clone ID Related Accession (Same CDS sequence)	XM_006525809.2 , XM_006525809.3
Accession Version	XM_006525809.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	gap junction delta-4 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525809.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGGC TGATCGTGGA GGTCTTGCTG AGGATGCTAG TGGTGGTCTT GGCAGGGTCA 
CCTATCTATG AGGATGAACA AGAGAGGTTT ATTTGCAACA CACTGCAACC AGGATGTGCC 
AACGTTTGCT ACGACCTCTT TTCCCCAGTG TCACCGCTGC GATTCTGGCT AGTGCAGAGC 
CTGGCCTTGC TTCTGCCTTC GGTGGTCTTT GGCACTTACA CCCTACACCG CGGTGCGAAG 
CTGGCTGCAG TGGGGGGAGC CTGCAGGCCC CAGGTGCCCG ACCTGTCTAC TGCCTACCTG 
GTGCACCTAC TGCTGCGCAT GCTGCTGGAG GCCGGGCTGG CCTTCCTGCA CTACTTTCTC 
TTTGGCTTTT CTGTGCCCGC CCGCGTGTCT TGCTCGCATG TACCCTGCTC AGGGGCTGTG 
GACTGCTACG TGTCGCGGCC CACGGAGAAG TCACTCCTGA TACTATTCTT TTGGGCAGTG 
AGTGCGCTAT CCTTCCTGCT CAGCTTGGCT GACCTGCTTT GGATCCTGCC GAGGAGAAAG 
ACACTGAGGA CCACGCAGTG GGTGAATGGA GAGGCTAGAC CAGTCTGTGA AGTACCTGCA 
CCTCCCCCTT GCCTCTTACA AAACCCCCAG GGCTATCTTA GCCAAGGTCA GGTGGACCAA 
GAGGACAGAC AGGAGGAACA AGTTGTGCCT GAGTTCCCCT GCATGTGGAC AGCAGGGCAG 
AGTGACAACA GCAATGTTGG TCAGGCCTGT GTGTCGGGGC TGCTGGAACA TTCAGACCAA 
GATGCTAGTG AGGCCACTTC CTCAGCTGGT GACAGGCTAA CAGTGGCTCA CACAGCACAT 
GAGCTCAGAT TCCACAGAGA GACTTCACTG GACCTGGGGG GCAAAAACAC CCAGGCAGAT 
GAACTCTCCT TGGCTACCCA GAGCCACCTG GCCAGACACA GTTCAGCCAG CAAGCCTCAA 
GCTCCATGCC GGCTGACCAC CTCAGGCAGT GCTCCCCATT TGAGAACCAA AAAATCTGAG 
TGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525872.1
CDS	85..1113
Translation	MIWLIVEVLLRMLVVVLAGSPIYEDEQERFICNTLQPGCANVCYDLFSPVSPLRFWLVQSLALLLPSVVFGTYTLHRGAKLAAVGGACRPQVPDLSTAYLVHLLLRMLLEAGLAFLHYFLFGFSVPARVSCSHVPCSGAVDCYVSRPTEKSLLILFFWAVSALSFLLSLADLLWILPRRKTLRTTQWVNGEARPVCEVPAPPPCLLQNPQGYLSQGQVDQEDRQEEQVVPEFPCMWTAGQSDNSNVGQACVSGLLEHSDQDASEATSSAGDRLTVAHTAHELRFHRETSLDLGGKNTQADELSLATQSHLARHSSASKPQAPCRLTTSGSAPHLRTKKSEWV

Target ORF information:

RefSeq Version	XM_006525809.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus gap junction protein, delta 4 (Gjd4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525809.3

ORF Insert Sequence:

ATGATCTGGC TGATCGTGGA GGTCTTGCTG AGGATGCTAG TGGTGGTCTT GGCAGGGTCA 
CCTATCTATG AGGATGAACA AGAGAGGTTT ATTTGCAACA CACTGCAACC AGGATGTGCC 
AACGTTTGCT ACGACCTCTT TTCCCCAGTG TCACCGCTGC GATTCTGGCT AGTGCAGAGC 
CTGGCCTTGC TTCTGCCTTC GGTGGTCTTT GGCACTTACA CCCTACACCG CGGTGCGAAG 
CTGGCTGCAG TGGGGGGAGC CTGCAGGCCC CAGGTGCCCG ACCTGTCTAC TGCCTACCTG 
GTGCACCTAC TGCTGCGCAT GCTGCTGGAG GCCGGGCTGG CCTTCCTGCA CTACTTTCTC 
TTTGGCTTTT CTGTGCCCGC CCGCGTGTCT TGCTCGCATG TACCCTGCTC AGGGGCTGTG 
GACTGCTACG TGTCGCGGCC CACGGAGAAG TCACTCCTGA TACTATTCTT TTGGGCAGTG 
AGTGCGCTAT CCTTCCTGCT CAGCTTGGCT GACCTGCTTT GGATCCTGCC GAGGAGAAAG 
ACACTGAGGA CCACGCAGTG GGTGAATGGA GAGGCTAGAC CAGTCTGTGA AGTACCTGCA 
CCTCCCCCTT GCCTCTTACA AAACCCCCAG GGCTATCTTA GCCAAGGTCA GGTGGACCAA 
GAGGACAGAC AGGAGGAACA AGTTGTGCCT GAGTTCCCCT GCATGTGGAC AGCAGGGCAG 
AGTGACAACA GCAATGTTGG TCAGGCCTGT GTGTCGGGGC TGCTGGAACA TTCAGACCAA 
GATGCTAGTG AGGCCACTTC CTCAGCTGGT GACAGGCTAA CAGTGGCTCA CACAGCACAT 
GAGCTCAGAT TCCACAGAGA GACTTCACTG GACCTGGGGG GCAAAAACAC CCAGGCAGAT 
GAACTCTCCT TGGCTACCCA GAGCCACCTG GCCAGACACA GTTCAGCCAG CAAGCCTCAA 
GCTCCATGCC GGCTGACCAC CTCAGGCAGT GCTCCCCATT TGAGAACCAA AAAATCTGAG 
TGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Connexin39 deficient mice display accelerated myogenesis and regeneration of skeletal muscle.
Experimental cell research317(8)1169-78(2011 May)
von Maltzahn J,Wulf V,Matern G,Willecke K

Spatiotemporal expression of connexin 39 and -43 during myoblast differentiation in cultured cells and in the mouse embryo.
Cell communication & adhesion13(1-2)55-60(2006 Jan-Apr)
von Maltzahn J,Wulf V,Willecke K

Cx37 and Cx43 localize to zona pellucida in mouse ovarian follicles.
Cell communication & adhesion13(1-2)61-77(2006 Jan-Apr)
Simon AM,Chen H,Jackson CL

The novel mouse connexin39 gene is expressed in developing striated muscle fibers.
Journal of cell science117(Pt 22)5381-92(2004 Oct)
von Maltzahn J,Euwens C,Willecke K,S?hl G