ENO2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ENO2
Entrez Gene ID	2026
Full Name	enolase 2
Synonyms	HEL-S-279,NSE
General protein information	Preferred Names enolase 2 Names gamma-enolase 2-phospho-D-glycerate hydro-lyase 2-phospho-D-glycerate hydrolyase enolase 2 (gamma, neuronal) epididymis secretory protein Li 279 neural enolase neuron specific gamma enolase neuron-specific enolase neuronal enriched enolase neurone-specific enolase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12p13.31
Summary	This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008].
Disorder MIM:	131360

mRNA and Protein(s)

mRNA	Protein	Name
NM_001975.2	NP_001966.1	gamma-enolase
NM_001975.3	NP_001966.1	gamma-enolase

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	LOC100911625	XP_006244698.1
Saccharomyces cerevisiae (baker's yeast)	ENO2	NP_012044.1
Kluyveromyces lactis	KLLA0A09185g	XP_451402.1
Neurospora crassa	NCU10042	XP_957959.1
Mus musculus (house mouse)	Eno2	NP_038537.1
Danio rerio (zebrafish)	eno2	NP_001003848.1
Magnaporthe oryzae (rice blast fungus)	MGG_10607	XP_003710703.1
Homo sapiens (human)	ENO2	NP_001966.1
	ENO2	XP_001110839.1
Gallus gallus (chicken)	ENO2	NP_990207.1
Saccharomyces cerevisiae (baker's yeast)	ENO1	NP_011770.3
Eremothecium gossypii	AGOS_AER294C	NP_985151.1
Schizosaccharomyces pombe (fission yeast)	eno101	NP_595903.1
Schizosaccharomyces pombe (fission yeast)	eno102	NP_001018769.2
Canis lupus familiaris (dog)	ENO2	XP_003639985.1
Bos taurus (cattle)	ENO2	NP_001094595.1
Rattus norvegicus (Norway rat)	Eno2	NP_647541.1

Gene Ontology

The following ENO2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ENO2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25601	NM_001975.3 Latest version!	Homo sapiens enolase 2 (ENO2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu25601	NM_001975.2	Homo sapiens enolase 2 (ENO2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ENO2 ( NM_001975.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001966.1 Homo sapiens enolase 2 (ENO2), mRNA.

Price & Availability↑

CloneID	OHu25601
Clone ID Related Accession (Same CDS sequence)	NM_001975.2 , NM_001975.3
Accession Version	NM_001975.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	gamma-enolase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U47924.1. On Nov 26, 2018 this sequence version replaced NM_001975.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.161086.1, SRR3476690.1104951.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000229277.6/ ENSP00000229277.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCCATAG AGAAGATCTG GGCCCGGGAG ATCCTGGACT CCCGCGGGAA CCCCACAGTG 
GAGGTGGATC TCTATACTGC CAAAGGTCTT TTCCGGGCTG CAGTGCCCAG TGGAGCCTCT 
ACGGGCATCT ATGAGGCCCT GGAGCTGAGG GATGGAGACA AACAGCGTTA CTTAGGCAAA 
GGTGTCCTGA AGGCAGTGGA CCACATCAAC TCCACCATCG CGCCAGCCCT CATCAGCTCA 
GGTCTCTCTG TGGTGGAGCA AGAGAAACTG GACAACCTGA TGCTGGAGTT GGATGGGACT 
GAGAACAAAT CCAAGTTTGG GGCCAATGCC ATCCTGGGTG TGTCTCTGGC CGTGTGTAAG 
GCAGGGGCAG CTGAGCGGGA ACTGCCCCTG TATCGCCACA TTGCTCAGCT GGCCGGGAAC 
TCAGACCTCA TCCTGCCTGT GCCGGCCTTC AACGTGATCA ATGGTGGCTC TCATGCTGGC 
AACAAGCTGG CCATGCAGGA GTTCATGATC CTCCCAGTGG GAGCTGAGAG CTTTCGGGAT 
GCCATGCGAC TAGGTGCAGA GGTCTACCAT ACACTCAAGG GAGTCATCAA GGACAAATAC 
GGCAAGGATG CCACCAATGT GGGGGATGAA GGTGGCTTTG CCCCCAATAT CCTGGAGAAC 
AGTGAAGCCT TGGAGCTGGT GAAGGAAGCC ATCGACAAGG CTGGCTACAC GGAAAAGATC 
GTTATTGGCA TGGATGTTGC TGCCTCAGAG TTTTATCGTG ATGGCAAATA TGACTTGGAC 
TTCAAGTCTC CCACTGATCC TTCCCGATAC ATCACTGGGG ACCAGCTGGG GGCACTCTAC 
CAGGACTTTG TCAGGGACTA TCCTGTGGTC TCCATTGAGG ACCCATTTGA CCAGGATGAT 
TGGGCTGCCT GGTCCAAGTT CACAGCCAAT GTAGGGATCC AGATTGTGGG TGATGACCTG 
ACAGTGACCA ACCCAAAACG TATTGAGCGG GCAGTGGAAG AAAAGGCCTG CAACTGTCTG 
CTGCTCAAGG TCAACCAGAT CGGCTCGGTC ACTGAAGCCA TCCAAGCGTG CAAGCTGGCC 
CAGGAGAATG GCTGGGGGGT CATGGTGAGT CATCGCTCAG GAGAGACTGA GGACACATTC 
ATTGCTGACC TGGTGGTGGG GCTGTGCACA GGCCAGATCA AGACTGGTGC CCCGTGCCGT 
TCTGAACGTC TGGCTAAATA CAACCAGCTC ATGAGAATTG AGGAAGAGCT GGGGGATGAA 
GCTCGCTTTG CCGGACATAA CTTCCGTAAT CCCAGTGTGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001966.1
CDS	93..1397
Translation	MSIEKIWAREILDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDGDKQRYLGKGVLKAVDHINSTIAPALISSGLSVVEQEKLDNLMLELDGTENKSKFGANAILGVSLAVCKAGAAERELPLYRHIAQLAGNSDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGAESFRDAMRLGAEVYHTLKGVIKDKYGKDATNVGDEGGFAPNILENSEALELVKEAIDKAGYTEKIVIGMDVAASEFYRDGKYDLDFKSPTDPSRYITGDQLGALYQDFVRDYPVVSIEDPFDQDDWAAWSKFTANVGIQIVGDDLTVTNPKRIERAVEEKACNCLLLKVNQIGSVTEAIQACKLAQENGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEELGDEARFAGHNFRNPSVL

Target ORF information:

RefSeq Version	NM_001975.3
Organism	Homo sapiens(human)
Definition	Homo sapiens enolase 2 (ENO2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001975.3

ORF Insert Sequence:

ATGTCCATAG AGAAGATCTG GGCCCGGGAG ATCCTGGACT CCCGCGGGAA CCCCACAGTG 
GAGGTGGATC TCTATACTGC CAAAGGTCTT TTCCGGGCTG CAGTGCCCAG TGGAGCCTCT 
ACGGGCATCT ATGAGGCCCT GGAGCTGAGG GATGGAGACA AACAGCGTTA CTTAGGCAAA 
GGTGTCCTGA AGGCAGTGGA CCACATCAAC TCCACCATCG CGCCAGCCCT CATCAGCTCA 
GGTCTCTCTG TGGTGGAGCA AGAGAAACTG GACAACCTGA TGCTGGAGTT GGATGGGACT 
GAGAACAAAT CCAAGTTTGG GGCCAATGCC ATCCTGGGTG TGTCTCTGGC CGTGTGTAAG 
GCAGGGGCAG CTGAGCGGGA ACTGCCCCTG TATCGCCACA TTGCTCAGCT GGCCGGGAAC 
TCAGACCTCA TCCTGCCTGT GCCGGCCTTC AACGTGATCA ATGGTGGCTC TCATGCTGGC 
AACAAGCTGG CCATGCAGGA GTTCATGATC CTCCCAGTGG GAGCTGAGAG CTTTCGGGAT 
GCCATGCGAC TAGGTGCAGA GGTCTACCAT ACACTCAAGG GAGTCATCAA GGACAAATAC 
GGCAAGGATG CCACCAATGT GGGGGATGAA GGTGGCTTTG CCCCCAATAT CCTGGAGAAC 
AGTGAAGCCT TGGAGCTGGT GAAGGAAGCC ATCGACAAGG CTGGCTACAC GGAAAAGATC 
GTTATTGGCA TGGATGTTGC TGCCTCAGAG TTTTATCGTG ATGGCAAATA TGACTTGGAC 
TTCAAGTCTC CCACTGATCC TTCCCGATAC ATCACTGGGG ACCAGCTGGG GGCACTCTAC 
CAGGACTTTG TCAGGGACTA TCCTGTGGTC TCCATTGAGG ACCCATTTGA CCAGGATGAT 
TGGGCTGCCT GGTCCAAGTT CACAGCCAAT GTAGGGATCC AGATTGTGGG TGATGACCTG 
ACAGTGACCA ACCCAAAACG TATTGAGCGG GCAGTGGAAG AAAAGGCCTG CAACTGTCTG 
CTGCTCAAGG TCAACCAGAT CGGCTCGGTC ACTGAAGCCA TCCAAGCGTG CAAGCTGGCC 
CAGGAGAATG GCTGGGGGGT CATGGTGAGT CATCGCTCAG GAGAGACTGA GGACACATTC 
ATTGCTGACC TGGTGGTGGG GCTGTGCACA GGCCAGATCA AGACTGGTGC CCCGTGCCGT 
TCTGAACGTC TGGCTAAATA CAACCAGCTC ATGAGAATTG AGGAAGAGCT GGGGGATGAA 
GCTCGCTTTG CCGGACATAA CTTCCGTAAT CCCAGTGTGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENO2 ( NM_001975.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001966.1 Homo sapiens enolase 2 (ENO2), mRNA.

Price & Availability↑

CloneID	OHu25601
Clone ID Related Accession (Same CDS sequence)	NM_001975.2 , NM_001975.3
Accession Version	NM_001975.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	gamma-enolase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X51956.1. On Oct 29, 2001 this sequence version replaced NM_001975.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.217540.1, SRR1803617.78047.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCCATAG AGAAGATCTG GGCCCGGGAG ATCCTGGACT CCCGCGGGAA CCCCACAGTG 
GAGGTGGATC TCTATACTGC CAAAGGTCTT TTCCGGGCTG CAGTGCCCAG TGGAGCCTCT 
ACGGGCATCT ATGAGGCCCT GGAGCTGAGG GATGGAGACA AACAGCGTTA CTTAGGCAAA 
GGTGTCCTGA AGGCAGTGGA CCACATCAAC TCCACCATCG CGCCAGCCCT CATCAGCTCA 
GGTCTCTCTG TGGTGGAGCA AGAGAAACTG GACAACCTGA TGCTGGAGTT GGATGGGACT 
GAGAACAAAT CCAAGTTTGG GGCCAATGCC ATCCTGGGTG TGTCTCTGGC CGTGTGTAAG 
GCAGGGGCAG CTGAGCGGGA ACTGCCCCTG TATCGCCACA TTGCTCAGCT GGCCGGGAAC 
TCAGACCTCA TCCTGCCTGT GCCGGCCTTC AACGTGATCA ATGGTGGCTC TCATGCTGGC 
AACAAGCTGG CCATGCAGGA GTTCATGATC CTCCCAGTGG GAGCTGAGAG CTTTCGGGAT 
GCCATGCGAC TAGGTGCAGA GGTCTACCAT ACACTCAAGG GAGTCATCAA GGACAAATAC 
GGCAAGGATG CCACCAATGT GGGGGATGAA GGTGGCTTTG CCCCCAATAT CCTGGAGAAC 
AGTGAAGCCT TGGAGCTGGT GAAGGAAGCC ATCGACAAGG CTGGCTACAC GGAAAAGATC 
GTTATTGGCA TGGATGTTGC TGCCTCAGAG TTTTATCGTG ATGGCAAATA TGACTTGGAC 
TTCAAGTCTC CCACTGATCC TTCCCGATAC ATCACTGGGG ACCAGCTGGG GGCACTCTAC 
CAGGACTTTG TCAGGGACTA TCCTGTGGTC TCCATTGAGG ACCCATTTGA CCAGGATGAT 
TGGGCTGCCT GGTCCAAGTT CACAGCCAAT GTAGGGATCC AGATTGTGGG TGATGACCTG 
ACAGTGACCA ACCCAAAACG TATTGAGCGG GCAGTGGAAG AAAAGGCCTG CAACTGTCTG 
CTGCTCAAGG TCAACCAGAT CGGCTCGGTC ACTGAAGCCA TCCAAGCGTG CAAGCTGGCC 
CAGGAGAATG GCTGGGGGGT CATGGTGAGT CATCGCTCAG GAGAGACTGA GGACACATTC 
ATTGCTGACC TGGTGGTGGG GCTGTGCACA GGCCAGATCA AGACTGGTGC CCCGTGCCGT 
TCTGAACGTC TGGCTAAATA CAACCAGCTC ATGAGAATTG AGGAAGAGCT GGGGGATGAA 
GCTCGCTTTG CCGGACATAA CTTCCGTAAT CCCAGTGTGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001966.1
CDS	223..1527
Translation	MSIEKIWAREILDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDGDKQRYLGKGVLKAVDHINSTIAPALISSGLSVVEQEKLDNLMLELDGTENKSKFGANAILGVSLAVCKAGAAERELPLYRHIAQLAGNSDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGAESFRDAMRLGAEVYHTLKGVIKDKYGKDATNVGDEGGFAPNILENSEALELVKEAIDKAGYTEKIVIGMDVAASEFYRDGKYDLDFKSPTDPSRYITGDQLGALYQDFVRDYPVVSIEDPFDQDDWAAWSKFTANVGIQIVGDDLTVTNPKRIERAVEEKACNCLLLKVNQIGSVTEAIQACKLAQENGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEELGDEARFAGHNFRNPSVL

Target ORF information:

RefSeq Version	NM_001975.2
Organism	Homo sapiens(human)
Definition	Homo sapiens enolase 2 (ENO2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001975.2

ORF Insert Sequence:

ATGTCCATAG AGAAGATCTG GGCCCGGGAG ATCCTGGACT CCCGCGGGAA CCCCACAGTG 
GAGGTGGATC TCTATACTGC CAAAGGTCTT TTCCGGGCTG CAGTGCCCAG TGGAGCCTCT 
ACGGGCATCT ATGAGGCCCT GGAGCTGAGG GATGGAGACA AACAGCGTTA CTTAGGCAAA 
GGTGTCCTGA AGGCAGTGGA CCACATCAAC TCCACCATCG CGCCAGCCCT CATCAGCTCA 
GGTCTCTCTG TGGTGGAGCA AGAGAAACTG GACAACCTGA TGCTGGAGTT GGATGGGACT 
GAGAACAAAT CCAAGTTTGG GGCCAATGCC ATCCTGGGTG TGTCTCTGGC CGTGTGTAAG 
GCAGGGGCAG CTGAGCGGGA ACTGCCCCTG TATCGCCACA TTGCTCAGCT GGCCGGGAAC 
TCAGACCTCA TCCTGCCTGT GCCGGCCTTC AACGTGATCA ATGGTGGCTC TCATGCTGGC 
AACAAGCTGG CCATGCAGGA GTTCATGATC CTCCCAGTGG GAGCTGAGAG CTTTCGGGAT 
GCCATGCGAC TAGGTGCAGA GGTCTACCAT ACACTCAAGG GAGTCATCAA GGACAAATAC 
GGCAAGGATG CCACCAATGT GGGGGATGAA GGTGGCTTTG CCCCCAATAT CCTGGAGAAC 
AGTGAAGCCT TGGAGCTGGT GAAGGAAGCC ATCGACAAGG CTGGCTACAC GGAAAAGATC 
GTTATTGGCA TGGATGTTGC TGCCTCAGAG TTTTATCGTG ATGGCAAATA TGACTTGGAC 
TTCAAGTCTC CCACTGATCC TTCCCGATAC ATCACTGGGG ACCAGCTGGG GGCACTCTAC 
CAGGACTTTG TCAGGGACTA TCCTGTGGTC TCCATTGAGG ACCCATTTGA CCAGGATGAT 
TGGGCTGCCT GGTCCAAGTT CACAGCCAAT GTAGGGATCC AGATTGTGGG TGATGACCTG 
ACAGTGACCA ACCCAAAACG TATTGAGCGG GCAGTGGAAG AAAAGGCCTG CAACTGTCTG 
CTGCTCAAGG TCAACCAGAT CGGCTCGGTC ACTGAAGCCA TCCAAGCGTG CAAGCTGGCC 
CAGGAGAATG GCTGGGGGGT CATGGTGAGT CATCGCTCAG GAGAGACTGA GGACACATTC 
ATTGCTGACC TGGTGGTGGG GCTGTGCACA GGCCAGATCA AGACTGGTGC CCCGTGCCGT 
TCTGAACGTC TGGCTAAATA CAACCAGCTC ATGAGAATTG AGGAAGAGCT GGGGGATGAA 
GCTCGCTTTG CCGGACATAA CTTCCGTAAT CCCAGTGTGC TGTGA