OTOS cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	OTOS
Entrez Gene ID	150677
Full Name	otospiralin
Synonyms	OTOSP
General protein information	Preferred Names otospiralin Names otospiralin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q37.3
Summary	Otospiralin is synthesized by nonsensory cells (fibrocytes) of the inner ear, and downregulation of otospiralin in guinea pigs leads to deafness (Lavigne-Rebillard et al., 2003 [PubMed 12687421]).[supplied by OMIM, Mar 2008].
Disorder MIM:	607877

mRNA and Protein(s)

mRNA	Protein	Name
NM_148961.3	NP_683764.1	otospiralin precursor
NM_148961.4	NP_683764.1	otospiralin precursor
XM_017003409.1	XP_016858898.1	otospiralin isoform X1
XM_017003410.1	XP_016858899.1	otospiralin isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	OTOS	NP_683764.1
Bos taurus (cattle)	OTOS	NP_001107997.1
Pan troglodytes (chimpanzee)	OTOS	XP_516192.3
Xenopus tropicalis (tropical clawed frog)	LOC101731047	XP_004920756.1
Canis lupus familiaris (dog)	OTOS	XP_534625.2
Rattus norvegicus (Norway rat)	Otos	NP_631927.1
Xenopus tropicalis (tropical clawed frog)	otos	NP_001165109.1
Mus musculus (house mouse)	Otos	NP_694754.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic variation in Otos is associated with cisplatin-induced ototoxicity.
Spracklen TF, Whitehorn H, Vorster AA, Ramma L, Dalvie S, Ramesar RS
Pharmacogenomics15(13)1667-76(2014 Sep)

Gene structure, chromosomal localization, and mutation screening of the human gene for the inner ear protein otospiralin.
Lavigne-Rebillard M, Delprat B, Surget MO, Griffoin JM, Weil D, Arbones M, Vincent R, Hamel CP
Neurogenetics4(3)137-40(2003 Apr)

Downregulation of otospiralin, a novel inner ear protein, causes hair cell degeneration and deafness.
Delprat B, Boulanger A, Wang J, Beaudoin V, Guitton MJ, Vent?o S, Dechesne CJ, Pujol R, Lavigne-Rebillard M, Puel JL, Hamel CP
The Journal of neuroscience : the official journal of the Society for Neuroscience22(5)1718-25(2002 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study indicates a potentially protective role for the variant G alleles of SNPs rs77124181 and rs2291767 in Otos against the development of cisplatin-induced ototoxicity
Title: Genetic variation in Otos is associated with cisplatin-induced ototoxicity.

OTOSP spans 1630 nucleotides, has 4 exons & encodes a 567-base cDNA. OTOSP is on chromosome 2 at position q37.3. Beside intronic polymorphisms, a rare variant (Pro7Leu) was found in deafness patients & controls, excluding it as a genetic deafness gene.
Title: Gene structure, chromosomal localization, and mutation screening of the human gene for the inner ear protein otospiralin.

The following OTOS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OTOS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31284	NM_148961.4 Latest version!	Homo sapiens otospiralin (OTOS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu31284	XM_017003410.1 Latest version!	Homo sapiens otospiralin (OTOS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu89049	XM_017003409.1 Latest version!	Homo sapiens otospiralin (OTOS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu31284	NM_148961.3	Homo sapiens otospiralin (OTOS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

OTOS ( NM_148961.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_683764.1 Homo sapiens otospiralin (OTOS), mRNA.

Price & Availability↑

CloneID	OHu31284
Clone ID Related Accession (Same CDS sequence)	NM_148961.3 , NM_148961.4 , XM_017003410.1
Accession Version	NM_148961.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	otospiralin precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX118794.1 and AI201810.1. On Feb 13, 2004 this sequence version replaced NM_148961.2. ##Evidence-Data-START## Transcript exon combination :: ERR279875.6691.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144120, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END##

ATGCAGGCCT GCATGGTGCC GGGGCTGGCC CTCTGCCTCC TACTGGGGCC TCTTGCAGGG 
GCCAAGCCTG TGCAGGAGGA AGGAGACCCT TACGCGGAGC TGCCGGCCAT GCCCTACTGG 
CCTTTCTCCA CCTCTGACTT CTGGAACTAT GTGCAGCACT TCCAGGCCCT GGGGGCCTAC 
CCCCAGATCG AGGACATGGC CCGAACCTTC TTTGCCCACT TCCCCCTGGG GAGCACGCTG 
GGCTTCCACG TTCCCTATCA GGAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_683764.1
CDS	156..425
Translation	MQACMVPGLALCLLLGPLAGAKPVQEEGDPYAELPAMPYWPFSTSDFWNYVQHFQALGAYPQIEDMARTFFAHFPLGSTLGFHVPYQED

Target ORF information:

RefSeq Version	NM_148961.3
Organism	Homo sapiens(human)
Definition	Homo sapiens otospiralin (OTOS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_148961.3

ORF Insert Sequence:

ATGCAGGCCT GCATGGTGCC GGGGCTGGCC CTCTGCCTCC TACTGGGGCC TCTTGCAGGG 
GCCAAGCCTG TGCAGGAGGA AGGAGACCCT TACGCGGAGC TGCCGGCCAT GCCCTACTGG 
CCTTTCTCCA CCTCTGACTT CTGGAACTAT GTGCAGCACT TCCAGGCCCT GGGGGCCTAC 
CCCCAGATCG AGGACATGGC CCGAACCTTC TTTGCCCACT TCCCCCTGGG GAGCACGCTG 
GGCTTCCACG TTCCCTATCA GGAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOS ( NM_148961.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_683764.1 Homo sapiens otospiralin (OTOS), mRNA.

Price & Availability↑

CloneID	OHu31284
Clone ID Related Accession (Same CDS sequence)	NM_148961.3 , NM_148961.4 , XM_017003410.1
Accession Version	NM_148961.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Homo sapiens(human)
Product	otospiralin precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC013469.8, BX118794.1 and AI201810.1. On Nov 22, 2018 this sequence version replaced NM_148961.3. ##Evidence-Data-START## Transcript exon combination :: ERR279875.6691.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144120, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal MANE Ensembl match :: ENST00000319460.2/ ENSP00000322486.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGGCCT GCATGGTGCC GGGGCTGGCC CTCTGCCTCC TACTGGGGCC TCTTGCAGGG 
GCCAAGCCTG TGCAGGAGGA AGGAGACCCT TACGCGGAGC TGCCGGCCAT GCCCTACTGG 
CCTTTCTCCA CCTCTGACTT CTGGAACTAT GTGCAGCACT TCCAGGCCCT GGGGGCCTAC 
CCCCAGATCG AGGACATGGC CCGAACCTTC TTTGCCCACT TCCCCCTGGG GAGCACGCTG 
GGCTTCCACG TTCCCTATCA GGAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_683764.1
CDS	158..427
Translation	MQACMVPGLALCLLLGPLAGAKPVQEEGDPYAELPAMPYWPFSTSDFWNYVQHFQALGAYPQIEDMARTFFAHFPLGSTLGFHVPYQED

Target ORF information:

RefSeq Version	NM_148961.4
Organism	Homo sapiens(human)
Definition	Homo sapiens otospiralin (OTOS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_148961.4

ORF Insert Sequence:

ATGCAGGCCT GCATGGTGCC GGGGCTGGCC CTCTGCCTCC TACTGGGGCC TCTTGCAGGG 
GCCAAGCCTG TGCAGGAGGA AGGAGACCCT TACGCGGAGC TGCCGGCCAT GCCCTACTGG 
CCTTTCTCCA CCTCTGACTT CTGGAACTAT GTGCAGCACT TCCAGGCCCT GGGGGCCTAC 
CCCCAGATCG AGGACATGGC CCGAACCTTC TTTGCCCACT TCCCCCTGGG GAGCACGCTG 
GGCTTCCACG TTCCCTATCA GGAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOS ( XM_017003410.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858899.1 Homo sapiens otospiralin (OTOS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu31284
Clone ID Related Accession (Same CDS sequence)	NM_148961.3 , NM_148961.4 , XM_017003410.1
Accession Version	XM_017003410.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	otospiralin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCCT GCATGGTGCC GGGGCTGGCC CTCTGCCTCC TACTGGGGCC TCTTGCAGGG 
GCCAAGCCTG TGCAGGAGGA AGGAGACCCT TACGCGGAGC TGCCGGCCAT GCCCTACTGG 
CCTTTCTCCA CCTCTGACTT CTGGAACTAT GTGCAGCACT TCCAGGCCCT GGGGGCCTAC 
CCCCAGATCG AGGACATGGC CCGAACCTTC TTTGCCCACT TCCCCCTGGG GAGCACGCTG 
GGCTTCCACG TTCCCTATCA GGAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858899.1
CDS	791..1060
Translation	MQACMVPGLALCLLLGPLAGAKPVQEEGDPYAELPAMPYWPFSTSDFWNYVQHFQALGAYPQIEDMARTFFAHFPLGSTLGFHVPYQED

Target ORF information:

RefSeq Version	XM_017003410.1
Organism	Homo sapiens(human)
Definition	Homo sapiens otospiralin (OTOS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003410.1

ORF Insert Sequence:

ATGCAGGCCT GCATGGTGCC GGGGCTGGCC CTCTGCCTCC TACTGGGGCC TCTTGCAGGG 
GCCAAGCCTG TGCAGGAGGA AGGAGACCCT TACGCGGAGC TGCCGGCCAT GCCCTACTGG 
CCTTTCTCCA CCTCTGACTT CTGGAACTAT GTGCAGCACT TCCAGGCCCT GGGGGCCTAC 
CCCCAGATCG AGGACATGGC CCGAACCTTC TTTGCCCACT TCCCCCTGGG GAGCACGCTG 
GGCTTCCACG TTCCCTATCA GGAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOS ( XM_017003409.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858898.1 Homo sapiens otospiralin (OTOS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu89049
Clone ID Related Accession (Same CDS sequence)	XM_017003409.1
Accession Version	XM_017003409.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	otospiralin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATTG CTAGGAAAAC AGTGGGCCTG AGTCGCAGAT CAGGAAGCAC CGGGAAGATG 
CAGGCCTGCA TGGTGCCGGG GCTGGCCCTC TGCCTCCTAC TGGGGCCTCT TGCAGGGGCC 
AAGCCTGTGC AGGAGGAAGG AGACCCTTAC GCGGAGCTGC CGGCCATGCC CTACTGGCCT 
TTCTCCACCT CTGACTTCTG GAACTATGTG CAGCACTTCC AGGCCCTGGG GGCCTACCCC 
CAGATCGAGG ACATGGCCCG AACCTTCTTT GCCCACTTCC CCCTGGGGAG CACGCTGGGC 
TTCCACGTTC CCTATCAGGA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858898.1
CDS	53..379
Translation	MEIARKTVGLSRRSGSTGKMQACMVPGLALCLLLGPLAGAKPVQEEGDPYAELPAMPYWPFSTSDFWNYVQHFQALGAYPQIEDMARTFFAHFPLGSTLGFHVPYQED

Target ORF information:

RefSeq Version	XM_017003409.1
Organism	Homo sapiens(human)
Definition	Homo sapiens otospiralin (OTOS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003409.1

ORF Insert Sequence:

ATGGAAATTG CTAGGAAAAC AGTGGGCCTG AGTCGCAGAT CAGGAAGCAC CGGGAAGATG 
CAGGCCTGCA TGGTGCCGGG GCTGGCCCTC TGCCTCCTAC TGGGGCCTCT TGCAGGGGCC 
AAGCCTGTGC AGGAGGAAGG AGACCCTTAC GCGGAGCTGC CGGCCATGCC CTACTGGCCT 
TTCTCCACCT CTGACTTCTG GAACTATGTG CAGCACTTCC AGGCCCTGGG GGCCTACCCC 
CAGATCGAGG ACATGGCCCG AACCTTCTTT GCCCACTTCC CCCTGGGGAG CACGCTGGGC 
TTCCACGTTC CCTATCAGGA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic variation in Otos is associated with cisplatin-induced ototoxicity.
Pharmacogenomics15(13)1667-76(2014 Sep)
Spracklen TF,Whitehorn H,Vorster AA,Ramma L,Dalvie S,Ramesar RS

Gene structure, chromosomal localization, and mutation screening of the human gene for the inner ear protein otospiralin.
Neurogenetics4(3)137-40(2003 Apr)
Lavigne-Rebillard M,Delprat B,Surget MO,Griffoin JM,Weil D,Arbones M,Vincent R,Hamel CP

Downregulation of otospiralin, a novel inner ear protein, causes hair cell degeneration and deafness.
The Journal of neuroscience : the official journal of the Society for Neuroscience22(5)1718-25(2002 Mar)
Delprat B,Boulanger A,Wang J,Beaudoin V,Guitton MJ,Vent?o S,Dechesne CJ,Pujol R,Lavigne-Rebillard M,Puel JL,Hamel CP

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OTOS cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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