COL12A1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017010252.2	XP_016865741.1	collagen alpha-1(XII) chain isoform X2
XM_011535436.2	XP_011533738.1	collagen alpha-1(XII) chain isoform X4
NM_080645.2	NP_542376.2	collagen alpha-1(XII) chain short isoform precursor
XM_017010252.1	XP_016865741.1	collagen alpha-1(XII) chain isoform X2
XM_011535434.1	XP_011533736.1	collagen alpha-1(XII) chain isoform X1
XM_011535435.1	XP_011533737.1	collagen alpha-1(XII) chain isoform X3
NM_004370.6	NP_004361.3	collagen alpha-1(XII) chain long isoform precursor
NM_080645.3	NP_542376.2	collagen alpha-1(XII) chain short isoform precursor
NM_004370.5	NP_004361.3	collagen alpha-1(XII) chain long isoform precursor

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04272	NM_004370.6 Latest version!	Homo sapiens collagen type XII alpha 1 chain (COL12A1), transcript variant long, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu05790	NM_080645.3 Latest version!	Homo sapiens collagen type XII alpha 1 chain (COL12A1), transcript variant short, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu57022	XM_011535434.1 Latest version!	Homo sapiens collagen type XII alpha 1 chain (COL12A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu57023	XM_011535435.1 Latest version!	Homo sapiens collagen type XII alpha 1 chain (COL12A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu57024	XM_011535436.2 Latest version!	Homo sapiens collagen type XII alpha 1 chain (COL12A1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu96293	XM_017010252.2 Latest version!	Homo sapiens collagen type XII alpha 1 chain (COL12A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OHu96293	XM_017010252.1	Homo sapiens collagen type XII alpha 1 (COL12A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu05790	NM_080645.2	Homo sapiens collagen type XII alpha 1 chain (COL12A1), transcript variant short, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu04272	NM_004370.5	Homo sapiens collagen type XII alpha 1 chain (COL12A1), transcript variant long, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

COL12A1 ( NM_004370.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_004361.3 Homo sapiens collagen type XII alpha 1 chain (COL12A1), transcript variant long, mRNA.

Price & Availability↑

CloneID	OHu04272
Clone ID Related Accession (Same CDS sequence)	NM_004370.6 , NM_004370.5
Accession Version	NM_004370.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	collagen alpha-1(XII) chain long isoform precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL080250.11, DA661773.1, BP286308.1, AL354664.12, U73778.1, AL096771.11 and AI752603.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_004370.5. Transcript Variant: This variant (long) represents the longer transcript and encodes the longer isoform (long). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U73778.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000322507.13/ ENSP00000325146.8 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGGAGTA GGCTTCCCCC AGCGCTTGCC GCCCTGGGCG CGGCCCTGCT CCTGTCTTCC 
ATTGAGGCAG AAGTTGACCC ACCTTCAGAC TTGAATTTTA AAATTATAGA TGAAAATACT 
GTTCATATGT CATGGGCAAA ACCAGTTGAT CCAATTGTGG GTTACAGAAT AACGGTGGAC 
CCTACAACGG ATGGGCCTAC TAAAGAATTT ACCCTTTCAG CTAGTACCAC TGAAACTTTA 
TTGTCAGAAC TTGTACCTGA AACAGAGTAT GTGGTGACAA TAACTTCATA TGATGAAGTA 
GAAGAAAGTG TACCAGTTAT AGGACAACTA ACAATTCAAA CAGGTAGTTC GACAAAGCCA 
GTGGAGAAGA AACCTGGAAA AACCGAGATA CAAAAATGCT CTGTCAGTGC CTGGACTGAT 
TTGGTTTTCC TCGTGGATGG CTCTTGGAGT GTGGGAAGAA ATAATTTCAA GTACATTTTA 
GACTTCATTG CTGCTCTTGT GTCTGCTTTT GACATTGGGG AAGAGAAGAC AAGAGTTGGA 
GTTGTTCAAT ACAGCTCTGA TACCAGGACT GAATTTAACT TAAATCAGTA CTACCAAAGG 
GATGAACTTC TTGCTGCAAT AAAAAAAATT CCATATAAAG GTGGCAACAC AATGACAGGG 
GATGCCATTG ATTATTTAGT TAAAAATACT TTCACGGAAT CTGCTGGGGC AAGAGTTGGC 
TTTCCTAAAG TGGCAATTAT TATTACGGAT GGAAAATCCC AGGATGAAGT GGAAATTCCA 
GCAAGAGAGC TTCGTAATGT TGGAGTTGAA GTTTTCTCCT TGGGCATTAA AGCTGCAGAT 
GCAAAAGAAC TCAAACAAAT TGCCTCCACA CCTTCACTGA ACCATGTTTT CAATGTGGCC 
AACTTTGATG CAATTGTGGA TATTCAGAAT GAGATCATCT CCCAGGTGTG CTCAGGTGTT 
GATGAACAAC TTGGTGAATT GGTTAGTGGA GAAGAAGTTG TTGAGCCTCC TTCAAATTTG 
ATTGCCATGG AAGTCTCTTC AAAATATGTT AAGCTAAATT GGAATCCATC TCCTAGTCCA 
GTGACTGGCT ACAAAGTCAT CCTCACACCA ATGACTGCAG GAAGCCGACA GCACGCTCTG 
AGTGTGGGGC CTCAGACAAC CACGCTCAGT GTTCGCGACC TCTCAGCAGA CACAGAATAC 
CAGATCAGTG TTTCCGCCAT GAAGGGAATG ACATCCAGTG AACCCATTTC AATAATGGAG 
AAGACTCAGC CAATGAAAGT TCAAGTGGAA TGCTCACGTG GTGTGGATAT AAAAGCCGAT 
ATTGTGTTTT TGGTTGATGG CTCCTATAGC ATTGGGATTG CAAACTTTGT TAAAGTTAGA 
GCCTTTTTGG AAGTTCTTGT AAAAAGTTTT GAAATTTCAC CAAATAGGGT CCAGATTAGT 
CTTGTGCAAT ACAGCCGGGA TCCTCATACT GAGTTCACTT TGAAAAAATT CACCAAAGTT 
GAAGATATAA TTGAAGCAAT AAACACCTTC CCTTACAGAG GAGGATCTAC AAATACTGGC 
AAAGCAATGA CTTATGTCAG AGAGAAAATA TTTGTGCCTA GCAAGGGATC AAGAAGCAAT 
GTGCCAAAGG TCATGATTCT TATCACGGAT GGGAAATCAT CAGATGCTTT CAGAGATCCT 
GCGATAAAAC TGAGGAATTC AGATGTTGAA ATCTTTGCAG TTGGTGTGAA GGATGCCGTT 
CGCTCAGAAT TGGAAGCTAT TGCCTCTCCT CCTGCAGAGA CCCATGTGTT CACAGTGGAA 
GATTTTGATG CTTTTCAGAG GATATCTTTT GAACTCACAC AGTCTATCTG CCTTAGAATT 
GAGCAAGAAT TGGCAGCTAT AAAGAAGAAA GCTTACGTCC CTCCAAAGGA TCTTAGTTTT 
TCAGAAGTGA CTTCTTATGG TTTCAAAACC AACTGGTCTC CAGCTGGAGA AAATGTTTTT 
TCATATCACA TCACCTACAA GGAAGCGGCT GGGGATGATG AGGTCACTGT GGTGGAGCCA 
GCATCGAGCA CCAGTGTTGT TCTCAGCAGC CTGAAGCCAG AGACCTTGTA TTTGGTCAAT 
GTGACTGCGG AGTATGAGGA TGGCTTCAGC ATTCCCTTAG CTGGAGAGGA GACCACCGAA 
GAAGTAAAAG GAGCACCTCG AAACCTAAAG GTGACAGATG AGACTACAGA TAGTTTCAAA 
ATTACTTGGA CTCAAGCTCC AGGGAGAGTT TTAAGATATC GAATTATATA TAGACCAGTT 
GCTGGTGGAG AGAGCAGAGA AGTTACCACC CCACCCAATC AGAGGAGGAG AACACTGGAG 
AACTTGATTC CAGACACGAA ATATGAAGTA TCTGTAATTC CTGAATATTT CTCAGGACCT 
GGTACTCCAT TAACTGGAAA TGCAGCCACT GAAGAAGTTA GAGGGAATCC AAGAGACTTA 
AGAGTTTCTG ACCCTACGAC GTCTACTATG AAATTATCTT GGAGTGGGGC ACCAGGAAAA 
GTGAAACAGT ATCTCGTCAC ATATACCCCA GTGGCAGGGG GTGAAACTCA AGAGGTCACT 
GTGAGGGGAG ATACAACCAA TACGGTGCTG CAGGGATTGA AGGAAGGGAC ACAATACGCC 
TTATCTGTGA CAGCCTTGTA TGCGTCTGGG GCTGGAGACG CCCTCTTTGG TGAAGGAACA 
ACACTTGAAG AACGTGGTTC TCCTCAAGAT TTAGTTACTA AAGACATCAC TGACACATCA 
ATTGGGGCTT ATTGGACATC TGCTCCAGGA ATGGTTCGCG GTTACAGGGT CTCATGGAAA 
TCACTTTATG ATGATGTTGA CACTGGAGAG AAAAATCTGC CTGAAGATGC AATTCATACG 
ATGATAGAAA ATCTGCAGCC AGAGACCAAA TACAGAATTT CAGTATTTGC CACTTACAGC 
AGTGGAGAAG GAGAACCTTT GACTGGAGAT GCCACAACTG AATTATCTCA AGATTCCAAA 
ACCCTGAAAG TAGATGAAGA AACAGAAAAC ACAATGAGAG TTACATGGAA ACCAGCACCA 
GGGAAAGTCG TCAACTACCG TGTTGTCTAT CGCCCTCATG GGAGAGGGAA GCAAATGGTT 
GCTAAGGTGC CCCCCACAGT CACTTCGACA GTGTTAAAGC GACTTCAGCC ACAGACCACA 
TATGACATCA CAGTTCTTCC TATTTACAAG ATGGGAGAAG GAAAGCTTAG GCAAGGATCA 
GGAACAACAG CTTCTCGGTT TAAGTCTCCT AGAAACCTCA AAACATCTGA CCCAACCATG 
TCAAGCTTCC GAGTGACTTG GGAGCCTGCC CCTGGGGAAG TGAAGGGTTA TAAAGTCACA 
TTCCACCCTA CGGGGGATGA CAGAAGACTG GGGGAGTTAG TGGTTGGACC CTATGACAAC 
ACAGTTGTTT TGGAGGAACT TAGGGCTGGT ACCACCTATA AAGTAAATGT TTTTGGAATG 
TTTGATGGAG GAGAAAGCTC ACCACTTGTT GGACAAGAAA TGACAACCCT TTCCGACACA 
ACTGTTATGC CAATTTTATC TTCTGGGATG GAGTGTCTCA CCAGAGCTGA GGCAGACATT 
GTGTTGCTGG TGGATGGATC ATGGAGCATC GGCCGGGCAA ATTTTAGAAC CGTGAGGAGT 
TTCATTTCTC GTATTGTGGA AGTCTTTGAC ATTGGCCCCA AAAGAGTACA AATTGCTCTT 
GCTCAGTATA GTGGGGATCC CAGAACAGAG TGGCAGTTAA ATGCACACAG AGACAAGAAG 
AGCTTGTTGC AAGCTGTGGC AAACTTGCCG TACAAAGGAG GCAATACTCT CACAGGCATG 
GCTTTGAATT TCATTCGCCA ACAGAACTTC AGGACCCAAG CTGGCATGAG ACCTCGAGCT 
CGAAAAATTG GTGTGCTCAT TACTGATGGA AAATCACAAG ACGATGTTGA AGCACCTTCA 
AAGAAACTCA AGGATGAGGG AGTGGAGCTG TTTGCTATTG GTATTAAAAA TGCTGATGAA 
GTCGAATTAA AGATGATTGC AACTGATCCT GATGATACCC ATGCATACAA TGTGGCAGAT 
TTTGAGTCAC TCTCCAGGAT AGTGGATGAT CTCACCATTA ATTTGTGTAA CAGTGTCAAA 
GGTCCAGGTG ATTTGGAAGC ACCTTCTAAC TTAGTTATTT CTGAGCGAAC CCATCGTTCT 
TTTAGAGTGA GCTGGACACC ACCTTCTGAC AGTGTGGATC GATATAAGGT GGAATACTAT 
CCAGTTTCTG GAGGGAAACG TCAAGAATTT TATGTGAGTC GAATGGAAAC TAGCACAGTG 
CTGAAAGATC TGAAACCTGA AACTGAATAT GTTGTCAATG TGTATTCTGT GGTAGAAGAT 
GAATATAGTG AGCCTCTGAA GGGGACAGAA AAAACCTTGC CAGTGCCTGT AGTCAGCCTG 
AATATTTATG ATGTTGGCCC TACCACCATG CATGTGCAGT GGCAGCCTGT GGGAGGAGCT 
ACTGGCTACA TCTTGTCATA CAAACCTGTT AAGGACACAG AGCCAACAAG ACCCAAAGAG 
GTGCGTTTGG GGCCAACAGT GAATGACATG CAGCTGACTG ACCTTGTTCC CAACACGGAG 
TATGCAGTCA CAGTCCAGGC TGTCCTGCAC GACCTCACTA GTGAACCTGT CACTGTTCGG 
GAAGTCACCT TGCCTTTACC CAGACCTCAG GATCTGAAAC TCAGAGATGT GACTCACAGC 
ACTATGAATG TCTTTTGGGA ACCTGTGCCT GGAAAAGTGC GTAAATATAT TGTTCGATAC 
AAAACACCAG AAGAGGATGT CAAAGAGGTA GAGGTGGACA GATCAGAGAC CAGCACTTCC 
CTCAAAGACC TCTTCTCACA GACCTTGTAC ACAGTCAGCG TTTCTGCAGT ACATGACGAG 
GGGGAGTCTC CTCCAGTGAC TGCTCAAGAA ACTACCCGAC CCGTGCCAGC CCCAACAAAC 
TTAAAGATTA CTGAAGTAAC ATCAGAGGGT TTCAGAGGGA CTTGGGATCA TGGAGCTTCA 
GATGTGTCTC TCTACAGAAT AACTTGGGCA CCTTTTGGAA GCTCAGATAA GATGGAGACC 
ATCTTAAATG GAGATGAAAA CACTTTGGTG TTCGAAAACC TGAACCCCAA CACCATCTAT 
GAAGTTTCCA TTACTGCCAT CTATCCTGAT GAGTCAGAAA GTGATGACCT GATTGGCAGT 
GAGCGCACAC TGCCTATCTT AACAACACAA GCTCCCAAAA GTGGCCCACG AAACCTTCAA 
GTGTACAATG CAACATCTAA CAGCCTGACT GTTAAGTGGG ATCCTGCTAG TGGTCGTGTG 
CAGAAATATA GGATCACTTA TCAGCCTTCC ACAGGGGAAG GCAATGAGCA AACGACCACA 
ATAGGAGGAC GGCAGAACAG TGTGGTCCTG CAGAAACTGA AGCCAGACAC TCCTTACACT 
ATCACCGTAT CCTCTCTGTA TCCTGATGGT GAAGGAGGTC GGATGACGGG AAGAGGCAAG 
ACCAAACCTC TAAACACTGT AAGGAACCTG AGAGTGTATG ACCCTTCTAC CAGCACCTTG 
AATGTCCGCT GGGACCATGC AGAGGGAAAT CCTCGTCAGT ACAAGCTCTT CTATGCACCA 
GCAGCAGGTG GTCCAGAGGA ACTGGTACCA ATCCCCGGGA ATACCAATTA TGCCATTCTT 
AGGAATCTGC AGCCAGATAC CTCATACACT GTGACTGTAG TTCCCGTTTA TACTGAAGGT 
GATGGGGGAC GCACATCAGA TACTGGAAGG ACATTGATGA GAGGACTGGC AAGAAATGTC 
CAAGTATACA ATCCTACACC TAACAGCCTC GATGTTCGCT GGGACCCTGC TCCAGGACCT 
GTGCTGCAAT ATCGCGTTGT GTATTCTCCT GTGGATGGCA CAAGACCCTC AGAATCTATA 
GTAGTGCCAG GAAACACGCG CATGGTGCAT CTGGAGCGGC TGATTCCGGA CACACTCTAT 
TCCGTGAACC TTGTGGCTCT GTACTCGGAT GGAGAGGGAA ATCCCAGCCC TGCCCAGGGC 
CGAACGCTAC CACGCAGTGG ACCAAGGAAC CTGAGAGTCT TTGGTGAAAC AACCAATAGC 
CTCTCGGTAG CCTGGGATCA TGCTGATGGG CCAGTTCAGC AGTACAGGAT CATCTATTCT 
CCCACTGTTG GTGATCCAAT TGATGAATAT ACCACAGTCC CAGGCAGAAG AAACAATGTA 
ATACTGCAGC CCCTGCAACC TGACACTCCA TATAAAATTA CTGTTATTGC TGTTTATGAA 
GATGGAGATG GTGGCCATCT AACAGGAAAT GGAAGAACTG TGGGACTCCT TCCTCCTCAG 
AACATACACA TCTCTGACGA ATGGTATACA AGATTCAGGG TGTCCTGGGA TCCTTCACCT 
TCTCCAGTTC TTGGATATAA AATAGTATAT AAGCCAGTGG GTTCCAATGA GCCCATGGAA 
GCCTTTGTTG GAGAAATGAC ATCATATACC TTACACAATC TCAATCCCAG CACCACCTAC 
GATGTGAATG TTTATGCTCA ATATGATTCT GGACTCAGTG TCCCCTTGAC AGATCAAGGC 
ACTACATTAT ATTTAAATGT AACAGATCTG AAAACTTACC AGATTGGGTG GGATACATTC 
TGTGTCAAAT GGTCACCTCA CCGGGCAGCC ACCTCCTACA GGCTAAAACT AAGCCCTGCA 
GATGGAACAA GGGGACAAGA AATTACAGTG CGTGGATCAG AAACCAGTCA CTGCTTCACT 
GGCCTTTCAC CAGACACTGA TTATGGTGTC ACTGTTTTTG TGCAGACACC AAATCTCGAG 
GGACCAGGAG TCTCTGTTAA AGAACATACC ACTGTGAAAC CAACAGAAGC CCCTACAGAG 
CCACCCACAC CTCCTCCCCC TCCCACCATT CCACCAGCCC GGGATGTATG CAAAGGGGCC 
AAGGCAGATA TTGTGTTCTT GACTGATGCC TCCTGGAGCA TTGGGGACGA TAATTTTAAC 
AAAGTTGTAA AATTCATCTT CAATACTGTG GGAGGCTTTG ATGAAATCAG TCCTGCTGGG 
ATTCAGGTTT CATTTGTGCA ATACAGCGAT GAGGTCAAGT CTGAGTTCAA GCTGAACACG 
TACAATGACA AGGCCCTAGC CCTTGGGGCC CTCCAGAATA TTAGGTACAG AGGAGGAAAC 
ACAAGAACAG GCAAGGCCCT CACGTTTATC AAGGAGAAAG TCTTGACTTG GGAGAGCGGC 
ATGAGGAAGA ATGTCCCTAA GGTGTTGGTT GTGGTCACGG ACGGTCGGTC CCAGGATGAG 
GTCAAGAAGG CGGCTTTGGT CATCCAGCAG TCAGGGTTCA GTGTCTTTGT AGTTGGTGTG 
GCTGATGTCG ACTACAATGA GCTTGCCAAC ATTGCCAGCA AACCAAGTGA ACGGCACGTG 
TTCATTGTGG ACGACTTTGA ATCTTTTGAG AAGATCGAAG ACAATCTTAT TACATTTGTT 
TGTGAAACTG CCACTTCAAG TTGTCCTCTC ATTTATTTGG ATGGCTACAC CTCACCAGGT 
TTTAAAATGC TTGAAGCATA CAACCTGACA GAAAAGAATT TTGCTTCTGT ACAAGGAGTA 
TCTTTGGAGT CAGGGTCTTT CCCCAGCTAC TCAGCATACA GGATTCAGAA GAATGCGTTT 
GTGAATCAGC CTACAGCAGA CCTACACCCA AATGGACTCC CTCCTTCATA CACGATTATA 
TTATTATTCA GACTTCTCCC AGAAACTCCC AGTGACCCTT TTGCAATTTG GCAAATCACA 
GACAGAGACT ACAAACCACA AGTTGGAGTG ATTGCAGATC CTTCTAGCAA GACGTTATCA 
TTCTTTAACA AGGATACAAG AGGCGAGGTG CAAACTGTTA CATTTGACAC AGAAGAAGTA 
AAGACATTAT TTTATGGAAG TTTTCACAAG GTTCATATTG TAGTGACCTC AAAAAGTGTT 
AAGATTTACA TTGACTGCTA TGAAATTATA GAAAAAGACA TCAAGGAAGC TGGAAATATA 
ACAACTGATG GTTATGAAAT TCTTGGAAAA CTCCTTAAAG GGGAAAGGAA ATCAGCCGCA 
TTCCAAATCC AGAGTTTTGA CATTGTCTGC AGTCCAGTGT GGACCAGTAG AGACAGATGC 
TGTGATATTC CCTCTAGGAG AGATGAGGGA AAATGCCCTG CTTTTCCAAA TTCTTGCACA 
TGTACACAGG ACAGCGTTGG ACCTCCAGGA CCTCCAGGCC CTGCAGGAGG ACCTGGTGCT 
AAAGGTCCCA GAGGTGAAAG AGGTATCAGT GGGGCAATTG GGCCCCCTGG TCCTCGTGGA 
GACATAGGTC CTCCAGGCCC CCAGGGTCCT CCAGGCCCTC AGGGACCCAA TGGACTCTCT 
ATTCCGGGAG AGCAAGGTCG CCAAGGGATG AAAGGTGATG CTGGAGAGCC AGGACTTCCA 
GGCCGAACAG GAACCCCAGG ATTACCTGGC CCACCAGGAC CAATGGGACC TCCAGGAGAC 
AGAGGCTTCA CTGGAAAAGA CGGTGCAATG GGACCCAGGG GCCCACCAGG GCCGCCGGGA 
AGCCCAGGCT CCCCAGGAGT CACAGGACCA AGTGGGAAGC CAGGAAAACC TGGAGATCAT 
GGCAGACCAG GTCCATCTGG GTTGAAAGGA GAAAAAGGTG ATAGGGGAGA CATTGCTTCC 
CAGAACATGA TGCGAGCAGT TGCAAGACAA GTCTGTGAAC AATTGATAAG TGGTCAGATG 
AACAGATTCA ATCAGATGCT GAATCAGATT CCAAATGATT ACCAGTCCAG TCGCAACCAG 
CCAGGCCCGC CGGGTCCACC GGGACCTCCT GGTAGCGCAG GAGCCAGAGG AGAACCTGGG 
CCTGGGGGGC GGCCAGGCTT CCCGGGCACA CCAGGGATGC AGGGACCCCC TGGGGAACGA 
GGTTTGCCAG GAGAGAAAGG TGAAAGGGGT ACTGGATCTT CAGGACCTCG GGGGCTGCCT 
GGCCCCCCAG GTCCACAAGG AGAATCCAGA ACAGGTCCAC CAGGGTCCAC AGGTTCAAGA 
GGTCCCCCTG GCCCCCCTGG CCGTCCTGGA AACTCAGGTA TCCGAGGACC CCCAGGTCCT 
CCTGGATACT GTGATTCTTC TCAGTGTGCC AGCATCCCAT ACAACGGGCA AGGCTATCCA 
GGTTCCGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004361.3
CDS	313..9504
Translation	MRSRLPPALAALGAALLLSSIEAEVDPPSDLNFKIIDENTVHMSWAKPVDPIVGYRITVDPTTDGPTKEFTLSASTTETLLSELVPETEYVVTITSYDEVEESVPVIGQLTIQTGSSTKPVEKKPGKTEIQKCSVSAWTDLVFLVDGSWSVGRNNFKYILDFIAALVSAFDIGEEKTRVGVVQYSSDTRTEFNLNQYYQRDELLAAIKKIPYKGGNTMTGDAIDYLVKNTFTESAGARVGFPKVAIIITDGKSQDEVEIPARELRNVGVEVFSLGIKAADAKELKQIASTPSLNHVFNVANFDAIVDIQNEIISQVCSGVDEQLGELVSGEEVVEPPSNLIAMEVSSKYVKLNWNPSPSPVTGYKVILTPMTAGSRQHALSVGPQTTTLSVRDLSADTEYQISVSAMKGMTSSEPISIMEKTQPMKVQVECSRGVDIKADIVFLVDGSYSIGIANFVKVRAFLEVLVKSFEISPNRVQISLVQYSRDPHTEFTLKKFTKVEDIIEAINTFPYRGGSTNTGKAMTYVREKIFVPSKGSRSNVPKVMILITDGKSSDAFRDPAIKLRNSDVEIFAVGVKDAVRSELEAIASPPAETHVFTVEDFDAFQRISFELTQSICLRIEQELAAIKKKAYVPPKDLSFSEVTSYGFKTNWSPAGENVFSYHITYKEAAGDDEVTVVEPASSTSVVLSSLKPETLYLVNVTAEYEDGFSIPLAGEETTEEVKGAPRNLKVTDETTDSFKITWTQAPGRVLRYRIIYRPVAGGESREVTTPPNQRRRTLENLIPDTKYEVSVIPEYFSGPGTPLTGNAATEEVRGNPRDLRVSDPTTSTMKLSWSGAPGKVKQYLVTYTPVAGGETQEVTVRGDTTNTVLQGLKEGTQYALSVTALYASGAGDALFGEGTTLEERGSPQDLVTKDITDTSIGAYWTSAPGMVRGYRVSWKSLYDDVDTGEKNLPEDAIHTMIENLQPETKYRISVFATYSSGEGEPLTGDATTELSQDSKTLKVDEETENTMRVTWKPAPGKVVNYRVVYRPHGRGKQMVAKVPPTVTSTVLKRLQPQTTYDITVLPIYKMGEGKLRQGSGTTASRFKSPRNLKTSDPTMSSFRVTWEPAPGEVKGYKVTFHPTGDDRRLGELVVGPYDNTVVLEELRAGTTYKVNVFGMFDGGESSPLVGQEMTTLSDTTVMPILSSGMECLTRAEADIVLLVDGSWSIGRANFRTVRSFISRIVEVFDIGPKRVQIALAQYSGDPRTEWQLNAHRDKKSLLQAVANLPYKGGNTLTGMALNFIRQQNFRTQAGMRPRARKIGVLITDGKSQDDVEAPSKKLKDEGVELFAIGIKNADEVELKMIATDPDDTHAYNVADFESLSRIVDDLTINLCNSVKGPGDLEAPSNLVISERTHRSFRVSWTPPSDSVDRYKVEYYPVSGGKRQEFYVSRMETSTVLKDLKPETEYVVNVYSVVEDEYSEPLKGTEKTLPVPVVSLNIYDVGPTTMHVQWQPVGGATGYILSYKPVKDTEPTRPKEVRLGPTVNDMQLTDLVPNTEYAVTVQAVLHDLTSEPVTVREVTLPLPRPQDLKLRDVTHSTMNVFWEPVPGKVRKYIVRYKTPEEDVKEVEVDRSETSTSLKDLFSQTLYTVSVSAVHDEGESPPVTAQETTRPVPAPTNLKITEVTSEGFRGTWDHGASDVSLYRITWAPFGSSDKMETILNGDENTLVFENLNPNTIYEVSITAIYPDESESDDLIGSERTLPILTTQAPKSGPRNLQVYNATSNSLTVKWDPASGRVQKYRITYQPSTGEGNEQTTTIGGRQNSVVLQKLKPDTPYTITVSSLYPDGEGGRMTGRGKTKPLNTVRNLRVYDPSTSTLNVRWDHAEGNPRQYKLFYAPAAGGPEELVPIPGNTNYAILRNLQPDTSYTVTVVPVYTEGDGGRTSDTGRTLMRGLARNVQVYNPTPNSLDVRWDPAPGPVLQYRVVYSPVDGTRPSESIVVPGNTRMVHLERLIPDTLYSVNLVALYSDGEGNPSPAQGRTLPRSGPRNLRVFGETTNSLSVAWDHADGPVQQYRIIYSPTVGDPIDEYTTVPGRRNNVILQPLQPDTPYKITVIAVYEDGDGGHLTGNGRTVGLLPPQNIHISDEWYTRFRVSWDPSPSPVLGYKIVYKPVGSNEPMEAFVGEMTSYTLHNLNPSTTYDVNVYAQYDSGLSVPLTDQGTTLYLNVTDLKTYQIGWDTFCVKWSPHRAATSYRLKLSPADGTRGQEITVRGSETSHCFTGLSPDTDYGVTVFVQTPNLEGPGVSVKEHTTVKPTEAPTEPPTPPPPPTIPPARDVCKGAKADIVFLTDASWSIGDDNFNKVVKFIFNTVGGFDEISPAGIQVSFVQYSDEVKSEFKLNTYNDKALALGALQNIRYRGGNTRTGKALTFIKEKVLTWESGMRKNVPKVLVVVTDGRSQDEVKKAALVIQQSGFSVFVVGVADVDYNELANIASKPSERHVFIVDDFESFEKIEDNLITFVCETATSSCPLIYLDGYTSPGFKMLEAYNLTEKNFASVQGVSLESGSFPSYSAYRIQKNAFVNQPTADLHPNGLPPSYTIILLFRLLPETPSDPFAIWQITDRDYKPQVGVIADPSSKTLSFFNKDTRGEVQTVTFDTEEVKTLFYGSFHKVHIVVTSKSVKIYIDCYEIIEKDIKEAGNITTDGYEILGKLLKGERKSAAFQIQSFDIVCSPVWTSRDRCCDIPSRRDEGKCPAFPNSCTCTQDSVGPPGPPGPAGGPGAKGPRGERGISGAIGPPGPRGDIGPPGPQGPPGPQGPNGLSIPGEQGRQGMKGDAGEPGLPGRTGTPGLPGPPGPMGPPGDRGFTGKDGAMGPRGPPGPPGSPGSPGVTGPSGKPGKPGDHGRPGPSGLKGEKGDRGDIASQNMMRAVARQVCEQLISGQMNRFNQMLNQIPNDYQSSRNQPGPPGPPGPPGSAGARGEPGPGGRPGFPGTPGMQGPPGERGLPGEKGERGTGSSGPRGLPGPPGPQGESRTGPPGSTGSRGPPGPPGRPGNSGIRGPPGPPGYCDSSQCASIPYNGQGYPGSG

Target ORF information:

RefSeq Version	NM_004370.6
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XII alpha 1 chain (COL12A1), transcript variant long, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004370.6

ORF Insert Sequence:

ATGCGGAGTA GGCTTCCCCC AGCGCTTGCC GCCCTGGGCG CGGCCCTGCT CCTGTCTTCC 
ATTGAGGCAG AAGTTGACCC ACCTTCAGAC TTGAATTTTA AAATTATAGA TGAAAATACT 
GTTCATATGT CATGGGCAAA ACCAGTTGAT CCAATTGTGG GTTACAGAAT AACGGTGGAC 
CCTACAACGG ATGGGCCTAC TAAAGAATTT ACCCTTTCAG CTAGTACCAC TGAAACTTTA 
TTGTCAGAAC TTGTACCTGA AACAGAGTAT GTGGTGACAA TAACTTCATA TGATGAAGTA 
GAAGAAAGTG TACCAGTTAT AGGACAACTA ACAATTCAAA CAGGTAGTTC GACAAAGCCA 
GTGGAGAAGA AACCTGGAAA AACCGAGATA CAAAAATGCT CTGTCAGTGC CTGGACTGAT 
TTGGTTTTCC TCGTGGATGG CTCTTGGAGT GTGGGAAGAA ATAATTTCAA GTACATTTTA 
GACTTCATTG CTGCTCTTGT GTCTGCTTTT GACATTGGGG AAGAGAAGAC AAGAGTTGGA 
GTTGTTCAAT ACAGCTCTGA TACCAGGACT GAATTTAACT TAAATCAGTA CTACCAAAGG 
GATGAACTTC TTGCTGCAAT AAAAAAAATT CCATATAAAG GTGGCAACAC AATGACAGGG 
GATGCCATTG ATTATTTAGT TAAAAATACT TTCACGGAAT CTGCTGGGGC AAGAGTTGGC 
TTTCCTAAAG TGGCAATTAT TATTACGGAT GGAAAATCCC AGGATGAAGT GGAAATTCCA 
GCAAGAGAGC TTCGTAATGT TGGAGTTGAA GTTTTCTCCT TGGGCATTAA AGCTGCAGAT 
GCAAAAGAAC TCAAACAAAT TGCCTCCACA CCTTCACTGA ACCATGTTTT CAATGTGGCC 
AACTTTGATG CAATTGTGGA TATTCAGAAT GAGATCATCT CCCAGGTGTG CTCAGGTGTT 
GATGAACAAC TTGGTGAATT GGTTAGTGGA GAAGAAGTTG TTGAGCCTCC TTCAAATTTG 
ATTGCCATGG AAGTCTCTTC AAAATATGTT AAGCTAAATT GGAATCCATC TCCTAGTCCA 
GTGACTGGCT ACAAAGTCAT CCTCACACCA ATGACTGCAG GAAGCCGACA GCACGCTCTG 
AGTGTGGGGC CTCAGACAAC CACGCTCAGT GTTCGCGACC TCTCAGCAGA CACAGAATAC 
CAGATCAGTG TTTCCGCCAT GAAGGGAATG ACATCCAGTG AACCCATTTC AATAATGGAG 
AAGACTCAGC CAATGAAAGT TCAAGTGGAA TGCTCACGTG GTGTGGATAT AAAAGCCGAT 
ATTGTGTTTT TGGTTGATGG CTCCTATAGC ATTGGGATTG CAAACTTTGT TAAAGTTAGA 
GCCTTTTTGG AAGTTCTTGT AAAAAGTTTT GAAATTTCAC CAAATAGGGT CCAGATTAGT 
CTTGTGCAAT ACAGCCGGGA TCCTCATACT GAGTTCACTT TGAAAAAATT CACCAAAGTT 
GAAGATATAA TTGAAGCAAT AAACACCTTC CCTTACAGAG GAGGATCTAC AAATACTGGC 
AAAGCAATGA CTTATGTCAG AGAGAAAATA TTTGTGCCTA GCAAGGGATC AAGAAGCAAT 
GTGCCAAAGG TCATGATTCT TATCACGGAT GGGAAATCAT CAGATGCTTT CAGAGATCCT 
GCGATAAAAC TGAGGAATTC AGATGTTGAA ATCTTTGCAG TTGGTGTGAA GGATGCCGTT 
CGCTCAGAAT TGGAAGCTAT TGCCTCTCCT CCTGCAGAGA CCCATGTGTT CACAGTGGAA 
GATTTTGATG CTTTTCAGAG GATATCTTTT GAACTCACAC AGTCTATCTG CCTTAGAATT 
GAGCAAGAAT TGGCAGCTAT AAAGAAGAAA GCTTACGTCC CTCCAAAGGA TCTTAGTTTT 
TCAGAAGTGA CTTCTTATGG TTTCAAAACC AACTGGTCTC CAGCTGGAGA AAATGTTTTT 
TCATATCACA TCACCTACAA GGAAGCGGCT GGGGATGATG AGGTCACTGT GGTGGAGCCA 
GCATCGAGCA CCAGTGTTGT TCTCAGCAGC CTGAAGCCAG AGACCTTGTA TTTGGTCAAT 
GTGACTGCGG AGTATGAGGA TGGCTTCAGC ATTCCCTTAG CTGGAGAGGA GACCACCGAA 
GAAGTAAAAG GAGCACCTCG AAACCTAAAG GTGACAGATG AGACTACAGA TAGTTTCAAA 
ATTACTTGGA CTCAAGCTCC AGGGAGAGTT TTAAGATATC GAATTATATA TAGACCAGTT 
GCTGGTGGAG AGAGCAGAGA AGTTACCACC CCACCCAATC AGAGGAGGAG AACACTGGAG 
AACTTGATTC CAGACACGAA ATATGAAGTA TCTGTAATTC CTGAATATTT CTCAGGACCT 
GGTACTCCAT TAACTGGAAA TGCAGCCACT GAAGAAGTTA GAGGGAATCC AAGAGACTTA 
AGAGTTTCTG ACCCTACGAC GTCTACTATG AAATTATCTT GGAGTGGGGC ACCAGGAAAA 
GTGAAACAGT ATCTCGTCAC ATATACCCCA GTGGCAGGGG GTGAAACTCA AGAGGTCACT 
GTGAGGGGAG ATACAACCAA TACGGTGCTG CAGGGATTGA AGGAAGGGAC ACAATACGCC 
TTATCTGTGA CAGCCTTGTA TGCGTCTGGG GCTGGAGACG CCCTCTTTGG TGAAGGAACA 
ACACTTGAAG AACGTGGTTC TCCTCAAGAT TTAGTTACTA AAGACATCAC TGACACATCA 
ATTGGGGCTT ATTGGACATC TGCTCCAGGA ATGGTTCGCG GTTACAGGGT CTCATGGAAA 
TCACTTTATG ATGATGTTGA CACTGGAGAG AAAAATCTGC CTGAAGATGC AATTCATACG 
ATGATAGAAA ATCTGCAGCC AGAGACCAAA TACAGAATTT CAGTATTTGC CACTTACAGC 
AGTGGAGAAG GAGAACCTTT GACTGGAGAT GCCACAACTG AATTATCTCA AGATTCCAAA 
ACCCTGAAAG TAGATGAAGA AACAGAAAAC ACAATGAGAG TTACATGGAA ACCAGCACCA 
GGGAAAGTCG TCAACTACCG TGTTGTCTAT CGCCCTCATG GGAGAGGGAA GCAAATGGTT 
GCTAAGGTGC CCCCCACAGT CACTTCGACA GTGTTAAAGC GACTTCAGCC ACAGACCACA 
TATGACATCA CAGTTCTTCC TATTTACAAG ATGGGAGAAG GAAAGCTTAG GCAAGGATCA 
GGAACAACAG CTTCTCGGTT TAAGTCTCCT AGAAACCTCA AAACATCTGA CCCAACCATG 
TCAAGCTTCC GAGTGACTTG GGAGCCTGCC CCTGGGGAAG TGAAGGGTTA TAAAGTCACA 
TTCCACCCTA CGGGGGATGA CAGAAGACTG GGGGAGTTAG TGGTTGGACC CTATGACAAC 
ACAGTTGTTT TGGAGGAACT TAGGGCTGGT ACCACCTATA AAGTAAATGT TTTTGGAATG 
TTTGATGGAG GAGAAAGCTC ACCACTTGTT GGACAAGAAA TGACAACCCT TTCCGACACA 
ACTGTTATGC CAATTTTATC TTCTGGGATG GAGTGTCTCA CCAGAGCTGA GGCAGACATT 
GTGTTGCTGG TGGATGGATC ATGGAGCATC GGCCGGGCAA ATTTTAGAAC CGTGAGGAGT 
TTCATTTCTC GTATTGTGGA AGTCTTTGAC ATTGGCCCCA AAAGAGTACA AATTGCTCTT 
GCTCAGTATA GTGGGGATCC CAGAACAGAG TGGCAGTTAA ATGCACACAG AGACAAGAAG 
AGCTTGTTGC AAGCTGTGGC AAACTTGCCG TACAAAGGAG GCAATACTCT CACAGGCATG 
GCTTTGAATT TCATTCGCCA ACAGAACTTC AGGACCCAAG CTGGCATGAG ACCTCGAGCT 
CGAAAAATTG GTGTGCTCAT TACTGATGGA AAATCACAAG ACGATGTTGA AGCACCTTCA 
AAGAAACTCA AGGATGAGGG AGTGGAGCTG TTTGCTATTG GTATTAAAAA TGCTGATGAA 
GTCGAATTAA AGATGATTGC AACTGATCCT GATGATACCC ATGCATACAA TGTGGCAGAT 
TTTGAGTCAC TCTCCAGGAT AGTGGATGAT CTCACCATTA ATTTGTGTAA CAGTGTCAAA 
GGTCCAGGTG ATTTGGAAGC ACCTTCTAAC TTAGTTATTT CTGAGCGAAC CCATCGTTCT 
TTTAGAGTGA GCTGGACACC ACCTTCTGAC AGTGTGGATC GATATAAGGT GGAATACTAT 
CCAGTTTCTG GAGGGAAACG TCAAGAATTT TATGTGAGTC GAATGGAAAC TAGCACAGTG 
CTGAAAGATC TGAAACCTGA AACTGAATAT GTTGTCAATG TGTATTCTGT GGTAGAAGAT 
GAATATAGTG AGCCTCTGAA GGGGACAGAA AAAACCTTGC CAGTGCCTGT AGTCAGCCTG 
AATATTTATG ATGTTGGCCC TACCACCATG CATGTGCAGT GGCAGCCTGT GGGAGGAGCT 
ACTGGCTACA TCTTGTCATA CAAACCTGTT AAGGACACAG AGCCAACAAG ACCCAAAGAG 
GTGCGTTTGG GGCCAACAGT GAATGACATG CAGCTGACTG ACCTTGTTCC CAACACGGAG 
TATGCAGTCA CAGTCCAGGC TGTCCTGCAC GACCTCACTA GTGAACCTGT CACTGTTCGG 
GAAGTCACCT TGCCTTTACC CAGACCTCAG GATCTGAAAC TCAGAGATGT GACTCACAGC 
ACTATGAATG TCTTTTGGGA ACCTGTGCCT GGAAAAGTGC GTAAATATAT TGTTCGATAC 
AAAACACCAG AAGAGGATGT CAAAGAGGTA GAGGTGGACA GATCAGAGAC CAGCACTTCC 
CTCAAAGACC TCTTCTCACA GACCTTGTAC ACAGTCAGCG TTTCTGCAGT ACATGACGAG 
GGGGAGTCTC CTCCAGTGAC TGCTCAAGAA ACTACCCGAC CCGTGCCAGC CCCAACAAAC 
TTAAAGATTA CTGAAGTAAC ATCAGAGGGT TTCAGAGGGA CTTGGGATCA TGGAGCTTCA 
GATGTGTCTC TCTACAGAAT AACTTGGGCA CCTTTTGGAA GCTCAGATAA GATGGAGACC 
ATCTTAAATG GAGATGAAAA CACTTTGGTG TTCGAAAACC TGAACCCCAA CACCATCTAT 
GAAGTTTCCA TTACTGCCAT CTATCCTGAT GAGTCAGAAA GTGATGACCT GATTGGCAGT 
GAGCGCACAC TGCCTATCTT AACAACACAA GCTCCCAAAA GTGGCCCACG AAACCTTCAA 
GTGTACAATG CAACATCTAA CAGCCTGACT GTTAAGTGGG ATCCTGCTAG TGGTCGTGTG 
CAGAAATATA GGATCACTTA TCAGCCTTCC ACAGGGGAAG GCAATGAGCA AACGACCACA 
ATAGGAGGAC GGCAGAACAG TGTGGTCCTG CAGAAACTGA AGCCAGACAC TCCTTACACT 
ATCACCGTAT CCTCTCTGTA TCCTGATGGT GAAGGAGGTC GGATGACGGG AAGAGGCAAG 
ACCAAACCTC TAAACACTGT AAGGAACCTG AGAGTGTATG ACCCTTCTAC CAGCACCTTG 
AATGTCCGCT GGGACCATGC AGAGGGAAAT CCTCGTCAGT ACAAGCTCTT CTATGCACCA 
GCAGCAGGTG GTCCAGAGGA ACTGGTACCA ATCCCCGGGA ATACCAATTA TGCCATTCTT 
AGGAATCTGC AGCCAGATAC CTCATACACT GTGACTGTAG TTCCCGTTTA TACTGAAGGT 
GATGGGGGAC GCACATCAGA TACTGGAAGG ACATTGATGA GAGGACTGGC AAGAAATGTC 
CAAGTATACA ATCCTACACC TAACAGCCTC GATGTTCGCT GGGACCCTGC TCCAGGACCT 
GTGCTGCAAT ATCGCGTTGT GTATTCTCCT GTGGATGGCA CAAGACCCTC AGAATCTATA 
GTAGTGCCAG GAAACACGCG CATGGTGCAT CTGGAGCGGC TGATTCCGGA CACACTCTAT 
TCCGTGAACC TTGTGGCTCT GTACTCGGAT GGAGAGGGAA ATCCCAGCCC TGCCCAGGGC 
CGAACGCTAC CACGCAGTGG ACCAAGGAAC CTGAGAGTCT TTGGTGAAAC AACCAATAGC 
CTCTCGGTAG CCTGGGATCA TGCTGATGGG CCAGTTCAGC AGTACAGGAT CATCTATTCT 
CCCACTGTTG GTGATCCAAT TGATGAATAT ACCACAGTCC CAGGCAGAAG AAACAATGTA 
ATACTGCAGC CCCTGCAACC TGACACTCCA TATAAAATTA CTGTTATTGC TGTTTATGAA 
GATGGAGATG GTGGCCATCT AACAGGAAAT GGAAGAACTG TGGGACTCCT TCCTCCTCAG 
AACATACACA TCTCTGACGA ATGGTATACA AGATTCAGGG TGTCCTGGGA TCCTTCACCT 
TCTCCAGTTC TTGGATATAA AATAGTATAT AAGCCAGTGG GTTCCAATGA GCCCATGGAA 
GCCTTTGTTG GAGAAATGAC ATCATATACC TTACACAATC TCAATCCCAG CACCACCTAC 
GATGTGAATG TTTATGCTCA ATATGATTCT GGACTCAGTG TCCCCTTGAC AGATCAAGGC 
ACTACATTAT ATTTAAATGT AACAGATCTG AAAACTTACC AGATTGGGTG GGATACATTC 
TGTGTCAAAT GGTCACCTCA CCGGGCAGCC ACCTCCTACA GGCTAAAACT AAGCCCTGCA 
GATGGAACAA GGGGACAAGA AATTACAGTG CGTGGATCAG AAACCAGTCA CTGCTTCACT 
GGCCTTTCAC CAGACACTGA TTATGGTGTC ACTGTTTTTG TGCAGACACC AAATCTCGAG 
GGACCAGGAG TCTCTGTTAA AGAACATACC ACTGTGAAAC CAACAGAAGC CCCTACAGAG 
CCACCCACAC CTCCTCCCCC TCCCACCATT CCACCAGCCC GGGATGTATG CAAAGGGGCC 
AAGGCAGATA TTGTGTTCTT GACTGATGCC TCCTGGAGCA TTGGGGACGA TAATTTTAAC 
AAAGTTGTAA AATTCATCTT CAATACTGTG GGAGGCTTTG ATGAAATCAG TCCTGCTGGG 
ATTCAGGTTT CATTTGTGCA ATACAGCGAT GAGGTCAAGT CTGAGTTCAA GCTGAACACG 
TACAATGACA AGGCCCTAGC CCTTGGGGCC CTCCAGAATA TTAGGTACAG AGGAGGAAAC 
ACAAGAACAG GCAAGGCCCT CACGTTTATC AAGGAGAAAG TCTTGACTTG GGAGAGCGGC 
ATGAGGAAGA ATGTCCCTAA GGTGTTGGTT GTGGTCACGG ACGGTCGGTC CCAGGATGAG 
GTCAAGAAGG CGGCTTTGGT CATCCAGCAG TCAGGGTTCA GTGTCTTTGT AGTTGGTGTG 
GCTGATGTCG ACTACAATGA GCTTGCCAAC ATTGCCAGCA AACCAAGTGA ACGGCACGTG 
TTCATTGTGG ACGACTTTGA ATCTTTTGAG AAGATCGAAG ACAATCTTAT TACATTTGTT 
TGTGAAACTG CCACTTCAAG TTGTCCTCTC ATTTATTTGG ATGGCTACAC CTCACCAGGT 
TTTAAAATGC TTGAAGCATA CAACCTGACA GAAAAGAATT TTGCTTCTGT ACAAGGAGTA 
TCTTTGGAGT CAGGGTCTTT CCCCAGCTAC TCAGCATACA GGATTCAGAA GAATGCGTTT 
GTGAATCAGC CTACAGCAGA CCTACACCCA AATGGACTCC CTCCTTCATA CACGATTATA 
TTATTATTCA GACTTCTCCC AGAAACTCCC AGTGACCCTT TTGCAATTTG GCAAATCACA 
GACAGAGACT ACAAACCACA AGTTGGAGTG ATTGCAGATC CTTCTAGCAA GACGTTATCA 
TTCTTTAACA AGGATACAAG AGGCGAGGTG CAAACTGTTA CATTTGACAC AGAAGAAGTA 
AAGACATTAT TTTATGGAAG TTTTCACAAG GTTCATATTG TAGTGACCTC AAAAAGTGTT 
AAGATTTACA TTGACTGCTA TGAAATTATA GAAAAAGACA TCAAGGAAGC TGGAAATATA 
ACAACTGATG GTTATGAAAT TCTTGGAAAA CTCCTTAAAG GGGAAAGGAA ATCAGCCGCA 
TTCCAAATCC AGAGTTTTGA CATTGTCTGC AGTCCAGTGT GGACCAGTAG AGACAGATGC 
TGTGATATTC CCTCTAGGAG AGATGAGGGA AAATGCCCTG CTTTTCCAAA TTCTTGCACA 
TGTACACAGG ACAGCGTTGG ACCTCCAGGA CCTCCAGGCC CTGCAGGAGG ACCTGGTGCT 
AAAGGTCCCA GAGGTGAAAG AGGTATCAGT GGGGCAATTG GGCCCCCTGG TCCTCGTGGA 
GACATAGGTC CTCCAGGCCC CCAGGGTCCT CCAGGCCCTC AGGGACCCAA TGGACTCTCT 
ATTCCGGGAG AGCAAGGTCG CCAAGGGATG AAAGGTGATG CTGGAGAGCC AGGACTTCCA 
GGCCGAACAG GAACCCCAGG ATTACCTGGC CCACCAGGAC CAATGGGACC TCCAGGAGAC 
AGAGGCTTCA CTGGAAAAGA CGGTGCAATG GGACCCAGGG GCCCACCAGG GCCGCCGGGA 
AGCCCAGGCT CCCCAGGAGT CACAGGACCA AGTGGGAAGC CAGGAAAACC TGGAGATCAT 
GGCAGACCAG GTCCATCTGG GTTGAAAGGA GAAAAAGGTG ATAGGGGAGA CATTGCTTCC 
CAGAACATGA TGCGAGCAGT TGCAAGACAA GTCTGTGAAC AATTGATAAG TGGTCAGATG 
AACAGATTCA ATCAGATGCT GAATCAGATT CCAAATGATT ACCAGTCCAG TCGCAACCAG 
CCAGGCCCGC CGGGTCCACC GGGACCTCCT GGTAGCGCAG GAGCCAGAGG AGAACCTGGG 
CCTGGGGGGC GGCCAGGCTT CCCGGGCACA CCAGGGATGC AGGGACCCCC TGGGGAACGA 
GGTTTGCCAG GAGAGAAAGG TGAAAGGGGT ACTGGATCTT CAGGACCTCG GGGGCTGCCT 
GGCCCCCCAG GTCCACAAGG AGAATCCAGA ACAGGTCCAC CAGGGTCCAC AGGTTCAAGA 
GGTCCCCCTG GCCCCCCTGG CCGTCCTGGA AACTCAGGTA TCCGAGGACC CCCAGGTCCT 
CCTGGATACT GTGATTCTTC TCAGTGTGCC AGCATCCCAT ACAACGGGCA AGGCTATCCA 
GGTTCCGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL12A1 ( NM_004370.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004361.3 Homo sapiens collagen type XII alpha 1 chain (COL12A1), transcript variant long, mRNA.

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CloneID	OHu04272
Clone ID Related Accession (Same CDS sequence)	NM_004370.6 , NM_004370.5
Accession Version	NM_004370.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	collagen alpha-1(XII) chain long isoform precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA661773.1, BP286308.1, AL080250.11, AL354664.12, U73778.1, AL096771.11 and AI752603.1. This sequence is a reference standard in the RefSeqGene project. On Apr 21, 2006 this sequence version replaced NM_004370.4. Transcript Variant: This variant (long) represents the longer transcript and encodes the longer isoform (long). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U73778.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGAGTA GGCTTCCCCC AGCGCTTGCC GCCCTGGGCG CGGCCCTGCT CCTGTCTTCC 
ATTGAGGCAG AAGTTGACCC ACCTTCAGAC TTGAATTTTA AAATTATAGA TGAAAATACT 
GTTCATATGT CATGGGCAAA ACCAGTTGAT CCAATTGTGG GTTACAGAAT AACGGTGGAC 
CCTACAACGG ATGGGCCTAC TAAAGAATTT ACCCTTTCAG CTAGTACCAC TGAAACTTTA 
TTGTCAGAAC TTGTACCTGA AACAGAGTAT GTGGTGACAA TAACTTCATA TGATGAAGTA 
GAAGAAAGTG TACCAGTTAT AGGACAACTA ACAATTCAAA CAGGTAGTTC GACAAAGCCA 
GTGGAGAAGA AACCTGGAAA AACCGAGATA CAAAAATGCT CTGTCAGTGC CTGGACTGAT 
TTGGTTTTCC TCGTGGATGG CTCTTGGAGT GTGGGAAGAA ATAATTTCAA GTACATTTTA 
GACTTCATTG CTGCTCTTGT GTCTGCTTTT GACATTGGGG AAGAGAAGAC AAGAGTTGGA 
GTTGTTCAAT ACAGCTCTGA TACCAGGACT GAATTTAACT TAAATCAGTA CTACCAAAGG 
GATGAACTTC TTGCTGCAAT AAAAAAAATT CCATATAAAG GTGGCAACAC AATGACAGGG 
GATGCCATTG ATTATTTAGT TAAAAATACT TTCACGGAAT CTGCTGGGGC AAGAGTTGGC 
TTTCCTAAAG TGGCAATTAT TATTACGGAT GGAAAATCCC AGGATGAAGT GGAAATTCCA 
GCAAGAGAGC TTCGTAATGT TGGAGTTGAA GTTTTCTCCT TGGGCATTAA AGCTGCAGAT 
GCAAAAGAAC TCAAACAAAT TGCCTCCACA CCTTCACTGA ACCATGTTTT CAATGTGGCC 
AACTTTGATG CAATTGTGGA TATTCAGAAT GAGATCATCT CCCAGGTGTG CTCAGGTGTT 
GATGAACAAC TTGGTGAATT GGTTAGTGGA GAAGAAGTTG TTGAGCCTCC TTCAAATTTG 
ATTGCCATGG AAGTCTCTTC AAAATATGTT AAGCTAAATT GGAATCCATC TCCTAGTCCA 
GTGACTGGCT ACAAAGTCAT CCTCACACCA ATGACTGCAG GAAGCCGACA GCACGCTCTG 
AGTGTGGGGC CTCAGACAAC CACGCTCAGT GTTCGCGACC TCTCAGCAGA CACAGAATAC 
CAGATCAGTG TTTCCGCCAT GAAGGGAATG ACATCCAGTG AACCCATTTC AATAATGGAG 
AAGACTCAGC CAATGAAAGT TCAAGTGGAA TGCTCACGTG GTGTGGATAT AAAAGCCGAT 
ATTGTGTTTT TGGTTGATGG CTCCTATAGC ATTGGGATTG CAAACTTTGT TAAAGTTAGA 
GCCTTTTTGG AAGTTCTTGT AAAAAGTTTT GAAATTTCAC CAAATAGGGT CCAGATTAGT 
CTTGTGCAAT ACAGCCGGGA TCCTCATACT GAGTTCACTT TGAAAAAATT CACCAAAGTT 
GAAGATATAA TTGAAGCAAT AAACACCTTC CCTTACAGAG GAGGATCTAC AAATACTGGC 
AAAGCAATGA CTTATGTCAG AGAGAAAATA TTTGTGCCTA GCAAGGGATC AAGAAGCAAT 
GTGCCAAAGG TCATGATTCT TATCACGGAT GGGAAATCAT CAGATGCTTT CAGAGATCCT 
GCGATAAAAC TGAGGAATTC AGATGTTGAA ATCTTTGCAG TTGGTGTGAA GGATGCCGTT 
CGCTCAGAAT TGGAAGCTAT TGCCTCTCCT CCTGCAGAGA CCCATGTGTT CACAGTGGAA 
GATTTTGATG CTTTTCAGAG GATATCTTTT GAACTCACAC AGTCTATCTG CCTTAGAATT 
GAGCAAGAAT TGGCAGCTAT AAAGAAGAAA GCTTACGTCC CTCCAAAGGA TCTTAGTTTT 
TCAGAAGTGA CTTCTTATGG TTTCAAAACC AACTGGTCTC CAGCTGGAGA AAATGTTTTT 
TCATATCACA TCACCTACAA GGAAGCGGCT GGGGATGATG AGGTCACTGT GGTGGAGCCA 
GCATCGAGCA CCAGTGTTGT TCTCAGCAGC CTGAAGCCAG AGACCTTGTA TTTGGTCAAT 
GTGACTGCGG AGTATGAGGA TGGCTTCAGC ATTCCCTTAG CTGGAGAGGA GACCACCGAA 
GAAGTAAAAG GAGCACCTCG AAACCTAAAG GTGACAGATG AGACTACAGA TAGTTTCAAA 
ATTACTTGGA CTCAAGCTCC AGGGAGAGTT TTAAGATATC GAATTATATA TAGACCAGTT 
GCTGGTGGAG AGAGCAGAGA AGTTACCACC CCACCCAATC AGAGGAGGAG AACACTGGAG 
AACTTGATTC CAGACACGAA ATATGAAGTA TCTGTAATTC CTGAATATTT CTCAGGACCT 
GGTACTCCAT TAACTGGAAA TGCAGCCACT GAAGAAGTTA GAGGGAATCC AAGAGACTTA 
AGAGTTTCTG ACCCTACGAC GTCTACTATG AAATTATCTT GGAGTGGGGC ACCAGGAAAA 
GTGAAACAGT ATCTCGTCAC ATATACCCCA GTGGCAGGGG GTGAAACTCA AGAGGTCACT 
GTGAGGGGAG ATACAACCAA TACGGTGCTG CAGGGATTGA AGGAAGGGAC ACAATACGCC 
TTATCTGTGA CAGCCTTGTA TGCGTCTGGG GCTGGAGACG CCCTCTTTGG TGAAGGAACA 
ACACTTGAAG AACGTGGTTC TCCTCAAGAT TTAGTTACTA AAGACATCAC TGACACATCA 
ATTGGGGCTT ATTGGACATC TGCTCCAGGA ATGGTTCGCG GTTACAGGGT CTCATGGAAA 
TCACTTTATG ATGATGTTGA CACTGGAGAG AAAAATCTGC CTGAAGATGC AATTCATACG 
ATGATAGAAA ATCTGCAGCC AGAGACCAAA TACAGAATTT CAGTATTTGC CACTTACAGC 
AGTGGAGAAG GAGAACCTTT GACTGGAGAT GCCACAACTG AATTATCTCA AGATTCCAAA 
ACCCTGAAAG TAGATGAAGA AACAGAAAAC ACAATGAGAG TTACATGGAA ACCAGCACCA 
GGGAAAGTCG TCAACTACCG TGTTGTCTAT CGCCCTCATG GGAGAGGGAA GCAAATGGTT 
GCTAAGGTGC CCCCCACAGT CACTTCGACA GTGTTAAAGC GACTTCAGCC ACAGACCACA 
TATGACATCA CAGTTCTTCC TATTTACAAG ATGGGAGAAG GAAAGCTTAG GCAAGGATCA 
GGAACAACAG CTTCTCGGTT TAAGTCTCCT AGAAACCTCA AAACATCTGA CCCAACCATG 
TCAAGCTTCC GAGTGACTTG GGAGCCTGCC CCTGGGGAAG TGAAGGGTTA TAAAGTCACA 
TTCCACCCTA CGGGGGATGA CAGAAGACTG GGGGAGTTAG TGGTTGGACC CTATGACAAC 
ACAGTTGTTT TGGAGGAACT TAGGGCTGGT ACCACCTATA AAGTAAATGT TTTTGGAATG 
TTTGATGGAG GAGAAAGCTC ACCACTTGTT GGACAAGAAA TGACAACCCT TTCCGACACA 
ACTGTTATGC CAATTTTATC TTCTGGGATG GAGTGTCTCA CCAGAGCTGA GGCAGACATT 
GTGTTGCTGG TGGATGGATC ATGGAGCATC GGCCGGGCAA ATTTTAGAAC CGTGAGGAGT 
TTCATTTCTC GTATTGTGGA AGTCTTTGAC ATTGGCCCCA AAAGAGTACA AATTGCTCTT 
GCTCAGTATA GTGGGGATCC CAGAACAGAG TGGCAGTTAA ATGCACACAG AGACAAGAAG 
AGCTTGTTGC AAGCTGTGGC AAACTTGCCG TACAAAGGAG GCAATACTCT CACAGGCATG 
GCTTTGAATT TCATTCGCCA ACAGAACTTC AGGACCCAAG CTGGCATGAG ACCTCGAGCT 
CGAAAAATTG GTGTGCTCAT TACTGATGGA AAATCACAAG ACGATGTTGA AGCACCTTCA 
AAGAAACTCA AGGATGAGGG AGTGGAGCTG TTTGCTATTG GTATTAAAAA TGCTGATGAA 
GTCGAATTAA AGATGATTGC AACTGATCCT GATGATACCC ATGCATACAA TGTGGCAGAT 
TTTGAGTCAC TCTCCAGGAT AGTGGATGAT CTCACCATTA ATTTGTGTAA CAGTGTCAAA 
GGTCCAGGTG ATTTGGAAGC ACCTTCTAAC TTAGTTATTT CTGAGCGAAC CCATCGTTCT 
TTTAGAGTGA GCTGGACACC ACCTTCTGAC AGTGTGGATC GATATAAGGT GGAATACTAT 
CCAGTTTCTG GAGGGAAACG TCAAGAATTT TATGTGAGTC GAATGGAAAC TAGCACAGTG 
CTGAAAGATC TGAAACCTGA AACTGAATAT GTTGTCAATG TGTATTCTGT GGTAGAAGAT 
GAATATAGTG AGCCTCTGAA GGGGACAGAA AAAACCTTGC CAGTGCCTGT AGTCAGCCTG 
AATATTTATG ATGTTGGCCC TACCACCATG CATGTGCAGT GGCAGCCTGT GGGAGGAGCT 
ACTGGCTACA TCTTGTCATA CAAACCTGTT AAGGACACAG AGCCAACAAG ACCCAAAGAG 
GTGCGTTTGG GGCCAACAGT GAATGACATG CAGCTGACTG ACCTTGTTCC CAACACGGAG 
TATGCAGTCA CAGTCCAGGC TGTCCTGCAC GACCTCACTA GTGAACCTGT CACTGTTCGG 
GAAGTCACCT TGCCTTTACC CAGACCTCAG GATCTGAAAC TCAGAGATGT GACTCACAGC 
ACTATGAATG TCTTTTGGGA ACCTGTGCCT GGAAAAGTGC GTAAATATAT TGTTCGATAC 
AAAACACCAG AAGAGGATGT CAAAGAGGTA GAGGTGGACA GATCAGAGAC CAGCACTTCC 
CTCAAAGACC TCTTCTCACA GACCTTGTAC ACAGTCAGCG TTTCTGCAGT ACATGACGAG 
GGGGAGTCTC CTCCAGTGAC TGCTCAAGAA ACTACCCGAC CCGTGCCAGC CCCAACAAAC 
TTAAAGATTA CTGAAGTAAC ATCAGAGGGT TTCAGAGGGA CTTGGGATCA TGGAGCTTCA 
GATGTGTCTC TCTACAGAAT AACTTGGGCA CCTTTTGGAA GCTCAGATAA GATGGAGACC 
ATCTTAAATG GAGATGAAAA CACTTTGGTG TTCGAAAACC TGAACCCCAA CACCATCTAT 
GAAGTTTCCA TTACTGCCAT CTATCCTGAT GAGTCAGAAA GTGATGACCT GATTGGCAGT 
GAGCGCACAC TGCCTATCTT AACAACACAA GCTCCCAAAA GTGGCCCACG AAACCTTCAA 
GTGTACAATG CAACATCTAA CAGCCTGACT GTTAAGTGGG ATCCTGCTAG TGGTCGTGTG 
CAGAAATATA GGATCACTTA TCAGCCTTCC ACAGGGGAAG GCAATGAGCA AACGACCACA 
ATAGGAGGAC GGCAGAACAG TGTGGTCCTG CAGAAACTGA AGCCAGACAC TCCTTACACT 
ATCACCGTAT CCTCTCTGTA TCCTGATGGT GAAGGAGGTC GGATGACGGG AAGAGGCAAG 
ACCAAACCTC TAAACACTGT AAGGAACCTG AGAGTGTATG ACCCTTCTAC CAGCACCTTG 
AATGTCCGCT GGGACCATGC AGAGGGAAAT CCTCGTCAGT ACAAGCTCTT CTATGCACCA 
GCAGCAGGTG GTCCAGAGGA ACTGGTACCA ATCCCCGGGA ATACCAATTA TGCCATTCTT 
AGGAATCTGC AGCCAGATAC CTCATACACT GTGACTGTAG TTCCCGTTTA TACTGAAGGT 
GATGGGGGAC GCACATCAGA TACTGGAAGG ACATTGATGA GAGGACTGGC AAGAAATGTC 
CAAGTATACA ATCCTACACC TAACAGCCTC GATGTTCGCT GGGACCCTGC TCCAGGACCT 
GTGCTGCAAT ATCGCGTTGT GTATTCTCCT GTGGATGGCA CAAGACCCTC AGAATCTATA 
GTAGTGCCAG GAAACACGCG CATGGTGCAT CTGGAGCGGC TGATTCCGGA CACACTCTAT 
TCCGTGAACC TTGTGGCTCT GTACTCGGAT GGAGAGGGAA ATCCCAGCCC TGCCCAGGGC 
CGAACGCTAC CACGCAGTGG ACCAAGGAAC CTGAGAGTCT TTGGTGAAAC AACCAATAGC 
CTCTCGGTAG CCTGGGATCA TGCTGATGGG CCAGTTCAGC AGTACAGGAT CATCTATTCT 
CCCACTGTTG GTGATCCAAT TGATGAATAT ACCACAGTCC CAGGCAGAAG AAACAATGTA 
ATACTGCAGC CCCTGCAACC TGACACTCCA TATAAAATTA CTGTTATTGC TGTTTATGAA 
GATGGAGATG GTGGCCATCT AACAGGAAAT GGAAGAACTG TGGGACTCCT TCCTCCTCAG 
AACATACACA TCTCTGACGA ATGGTATACA AGATTCAGGG TGTCCTGGGA TCCTTCACCT 
TCTCCAGTTC TTGGATATAA AATAGTATAT AAGCCAGTGG GTTCCAATGA GCCCATGGAA 
GCCTTTGTTG GAGAAATGAC ATCATATACC TTACACAATC TCAATCCCAG CACCACCTAC 
GATGTGAATG TTTATGCTCA ATATGATTCT GGACTCAGTG TCCCCTTGAC AGATCAAGGC 
ACTACATTAT ATTTAAATGT AACAGATCTG AAAACTTACC AGATTGGGTG GGATACATTC 
TGTGTCAAAT GGTCACCTCA CCGGGCAGCC ACCTCCTACA GGCTAAAACT AAGCCCTGCA 
GATGGAACAA GGGGACAAGA AATTACAGTG CGTGGATCAG AAACCAGTCA CTGCTTCACT 
GGCCTTTCAC CAGACACTGA TTATGGTGTC ACTGTTTTTG TGCAGACACC AAATCTCGAG 
GGACCAGGAG TCTCTGTTAA AGAACATACC ACTGTGAAAC CAACAGAAGC CCCTACAGAG 
CCACCCACAC CTCCTCCCCC TCCCACCATT CCACCAGCCC GGGATGTATG CAAAGGGGCC 
AAGGCAGATA TTGTGTTCTT GACTGATGCC TCCTGGAGCA TTGGGGACGA TAATTTTAAC 
AAAGTTGTAA AATTCATCTT CAATACTGTG GGAGGCTTTG ATGAAATCAG TCCTGCTGGG 
ATTCAGGTTT CATTTGTGCA ATACAGCGAT GAGGTCAAGT CTGAGTTCAA GCTGAACACG 
TACAATGACA AGGCCCTAGC CCTTGGGGCC CTCCAGAATA TTAGGTACAG AGGAGGAAAC 
ACAAGAACAG GCAAGGCCCT CACGTTTATC AAGGAGAAAG TCTTGACTTG GGAGAGCGGC 
ATGAGGAAGA ATGTCCCTAA GGTGTTGGTT GTGGTCACGG ACGGTCGGTC CCAGGATGAG 
GTCAAGAAGG CGGCTTTGGT CATCCAGCAG TCAGGGTTCA GTGTCTTTGT AGTTGGTGTG 
GCTGATGTCG ACTACAATGA GCTTGCCAAC ATTGCCAGCA AACCAAGTGA ACGGCACGTG 
TTCATTGTGG ACGACTTTGA ATCTTTTGAG AAGATCGAAG ACAATCTTAT TACATTTGTT 
TGTGAAACTG CCACTTCAAG TTGTCCTCTC ATTTATTTGG ATGGCTACAC CTCACCAGGT 
TTTAAAATGC TTGAAGCATA CAACCTGACA GAAAAGAATT TTGCTTCTGT ACAAGGAGTA 
TCTTTGGAGT CAGGGTCTTT CCCCAGCTAC TCAGCATACA GGATTCAGAA GAATGCGTTT 
GTGAATCAGC CTACAGCAGA CCTACACCCA AATGGACTCC CTCCTTCATA CACGATTATA 
TTATTATTCA GACTTCTCCC AGAAACTCCC AGTGACCCTT TTGCAATTTG GCAAATCACA 
GACAGAGACT ACAAACCACA AGTTGGAGTG ATTGCAGATC CTTCTAGCAA GACGTTATCA 
TTCTTTAACA AGGATACAAG AGGCGAGGTG CAAACTGTTA CATTTGACAC AGAAGAAGTA 
AAGACATTAT TTTATGGAAG TTTTCACAAG GTTCATATTG TAGTGACCTC AAAAAGTGTT 
AAGATTTACA TTGACTGCTA TGAAATTATA GAAAAAGACA TCAAGGAAGC TGGAAATATA 
ACAACTGATG GTTATGAAAT TCTTGGAAAA CTCCTTAAAG GGGAAAGGAA ATCAGCCGCA 
TTCCAAATCC AGAGTTTTGA CATTGTCTGC AGTCCAGTGT GGACCAGTAG AGACAGATGC 
TGTGATATTC CCTCTAGGAG AGATGAGGGA AAATGCCCTG CTTTTCCAAA TTCTTGCACA 
TGTACACAGG ACAGCGTTGG ACCTCCAGGA CCTCCAGGCC CTGCAGGAGG ACCTGGTGCT 
AAAGGTCCCA GAGGTGAAAG AGGTATCAGT GGGGCAATTG GGCCCCCTGG TCCTCGTGGA 
GACATAGGTC CTCCAGGCCC CCAGGGTCCT CCAGGCCCTC AGGGACCCAA TGGACTCTCT 
ATTCCGGGAG AGCAAGGTCG CCAAGGGATG AAAGGTGATG CTGGAGAGCC AGGACTTCCA 
GGCCGAACAG GAACCCCAGG ATTACCTGGC CCACCAGGAC CAATGGGACC TCCAGGAGAC 
AGAGGCTTCA CTGGAAAAGA CGGTGCAATG GGACCCAGGG GCCCACCAGG GCCGCCGGGA 
AGCCCAGGCT CCCCAGGAGT CACAGGACCA AGTGGGAAGC CAGGAAAACC TGGAGATCAT 
GGCAGACCAG GTCCATCTGG GTTGAAAGGA GAAAAAGGTG ATAGGGGAGA CATTGCTTCC 
CAGAACATGA TGCGAGCAGT TGCAAGACAA GTCTGTGAAC AATTGATAAG TGGTCAGATG 
AACAGATTCA ATCAGATGCT GAATCAGATT CCAAATGATT ACCAGTCCAG TCGCAACCAG 
CCAGGCCCGC CGGGTCCACC GGGACCTCCT GGTAGCGCAG GAGCCAGAGG AGAACCTGGG 
CCTGGGGGGC GGCCAGGCTT CCCGGGCACA CCAGGGATGC AGGGACCCCC TGGGGAACGA 
GGTTTGCCAG GAGAGAAAGG TGAAAGGGGT ACTGGATCTT CAGGACCTCG GGGGCTGCCT 
GGCCCCCCAG GTCCACAAGG AGAATCCAGA ACAGGTCCAC CAGGGTCCAC AGGTTCAAGA 
GGTCCCCCTG GCCCCCCTGG CCGTCCTGGA AACTCAGGTA TCCGAGGACC CCCAGGTCCT 
CCTGGATACT GTGATTCTTC TCAGTGTGCC AGCATCCCAT ACAACGGGCA AGGCTATCCA 
GGTTCCGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004361.3
CDS	167..9358
Translation	MRSRLPPALAALGAALLLSSIEAEVDPPSDLNFKIIDENTVHMSWAKPVDPIVGYRITVDPTTDGPTKEFTLSASTTETLLSELVPETEYVVTITSYDEVEESVPVIGQLTIQTGSSTKPVEKKPGKTEIQKCSVSAWTDLVFLVDGSWSVGRNNFKYILDFIAALVSAFDIGEEKTRVGVVQYSSDTRTEFNLNQYYQRDELLAAIKKIPYKGGNTMTGDAIDYLVKNTFTESAGARVGFPKVAIIITDGKSQDEVEIPARELRNVGVEVFSLGIKAADAKELKQIASTPSLNHVFNVANFDAIVDIQNEIISQVCSGVDEQLGELVSGEEVVEPPSNLIAMEVSSKYVKLNWNPSPSPVTGYKVILTPMTAGSRQHALSVGPQTTTLSVRDLSADTEYQISVSAMKGMTSSEPISIMEKTQPMKVQVECSRGVDIKADIVFLVDGSYSIGIANFVKVRAFLEVLVKSFEISPNRVQISLVQYSRDPHTEFTLKKFTKVEDIIEAINTFPYRGGSTNTGKAMTYVREKIFVPSKGSRSNVPKVMILITDGKSSDAFRDPAIKLRNSDVEIFAVGVKDAVRSELEAIASPPAETHVFTVEDFDAFQRISFELTQSICLRIEQELAAIKKKAYVPPKDLSFSEVTSYGFKTNWSPAGENVFSYHITYKEAAGDDEVTVVEPASSTSVVLSSLKPETLYLVNVTAEYEDGFSIPLAGEETTEEVKGAPRNLKVTDETTDSFKITWTQAPGRVLRYRIIYRPVAGGESREVTTPPNQRRRTLENLIPDTKYEVSVIPEYFSGPGTPLTGNAATEEVRGNPRDLRVSDPTTSTMKLSWSGAPGKVKQYLVTYTPVAGGETQEVTVRGDTTNTVLQGLKEGTQYALSVTALYASGAGDALFGEGTTLEERGSPQDLVTKDITDTSIGAYWTSAPGMVRGYRVSWKSLYDDVDTGEKNLPEDAIHTMIENLQPETKYRISVFATYSSGEGEPLTGDATTELSQDSKTLKVDEETENTMRVTWKPAPGKVVNYRVVYRPHGRGKQMVAKVPPTVTSTVLKRLQPQTTYDITVLPIYKMGEGKLRQGSGTTASRFKSPRNLKTSDPTMSSFRVTWEPAPGEVKGYKVTFHPTGDDRRLGELVVGPYDNTVVLEELRAGTTYKVNVFGMFDGGESSPLVGQEMTTLSDTTVMPILSSGMECLTRAEADIVLLVDGSWSIGRANFRTVRSFISRIVEVFDIGPKRVQIALAQYSGDPRTEWQLNAHRDKKSLLQAVANLPYKGGNTLTGMALNFIRQQNFRTQAGMRPRARKIGVLITDGKSQDDVEAPSKKLKDEGVELFAIGIKNADEVELKMIATDPDDTHAYNVADFESLSRIVDDLTINLCNSVKGPGDLEAPSNLVISERTHRSFRVSWTPPSDSVDRYKVEYYPVSGGKRQEFYVSRMETSTVLKDLKPETEYVVNVYSVVEDEYSEPLKGTEKTLPVPVVSLNIYDVGPTTMHVQWQPVGGATGYILSYKPVKDTEPTRPKEVRLGPTVNDMQLTDLVPNTEYAVTVQAVLHDLTSEPVTVREVTLPLPRPQDLKLRDVTHSTMNVFWEPVPGKVRKYIVRYKTPEEDVKEVEVDRSETSTSLKDLFSQTLYTVSVSAVHDEGESPPVTAQETTRPVPAPTNLKITEVTSEGFRGTWDHGASDVSLYRITWAPFGSSDKMETILNGDENTLVFENLNPNTIYEVSITAIYPDESESDDLIGSERTLPILTTQAPKSGPRNLQVYNATSNSLTVKWDPASGRVQKYRITYQPSTGEGNEQTTTIGGRQNSVVLQKLKPDTPYTITVSSLYPDGEGGRMTGRGKTKPLNTVRNLRVYDPSTSTLNVRWDHAEGNPRQYKLFYAPAAGGPEELVPIPGNTNYAILRNLQPDTSYTVTVVPVYTEGDGGRTSDTGRTLMRGLARNVQVYNPTPNSLDVRWDPAPGPVLQYRVVYSPVDGTRPSESIVVPGNTRMVHLERLIPDTLYSVNLVALYSDGEGNPSPAQGRTLPRSGPRNLRVFGETTNSLSVAWDHADGPVQQYRIIYSPTVGDPIDEYTTVPGRRNNVILQPLQPDTPYKITVIAVYEDGDGGHLTGNGRTVGLLPPQNIHISDEWYTRFRVSWDPSPSPVLGYKIVYKPVGSNEPMEAFVGEMTSYTLHNLNPSTTYDVNVYAQYDSGLSVPLTDQGTTLYLNVTDLKTYQIGWDTFCVKWSPHRAATSYRLKLSPADGTRGQEITVRGSETSHCFTGLSPDTDYGVTVFVQTPNLEGPGVSVKEHTTVKPTEAPTEPPTPPPPPTIPPARDVCKGAKADIVFLTDASWSIGDDNFNKVVKFIFNTVGGFDEISPAGIQVSFVQYSDEVKSEFKLNTYNDKALALGALQNIRYRGGNTRTGKALTFIKEKVLTWESGMRKNVPKVLVVVTDGRSQDEVKKAALVIQQSGFSVFVVGVADVDYNELANIASKPSERHVFIVDDFESFEKIEDNLITFVCETATSSCPLIYLDGYTSPGFKMLEAYNLTEKNFASVQGVSLESGSFPSYSAYRIQKNAFVNQPTADLHPNGLPPSYTIILLFRLLPETPSDPFAIWQITDRDYKPQVGVIADPSSKTLSFFNKDTRGEVQTVTFDTEEVKTLFYGSFHKVHIVVTSKSVKIYIDCYEIIEKDIKEAGNITTDGYEILGKLLKGERKSAAFQIQSFDIVCSPVWTSRDRCCDIPSRRDEGKCPAFPNSCTCTQDSVGPPGPPGPAGGPGAKGPRGERGISGAIGPPGPRGDIGPPGPQGPPGPQGPNGLSIPGEQGRQGMKGDAGEPGLPGRTGTPGLPGPPGPMGPPGDRGFTGKDGAMGPRGPPGPPGSPGSPGVTGPSGKPGKPGDHGRPGPSGLKGEKGDRGDIASQNMMRAVARQVCEQLISGQMNRFNQMLNQIPNDYQSSRNQPGPPGPPGPPGSAGARGEPGPGGRPGFPGTPGMQGPPGERGLPGEKGERGTGSSGPRGLPGPPGPQGESRTGPPGSTGSRGPPGPPGRPGNSGIRGPPGPPGYCDSSQCASIPYNGQGYPGSG

Target ORF information:

RefSeq Version	NM_004370.5
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XII alpha 1 chain (COL12A1), transcript variant long, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004370.5

ORF Insert Sequence:

ATGCGGAGTA GGCTTCCCCC AGCGCTTGCC GCCCTGGGCG CGGCCCTGCT CCTGTCTTCC 
ATTGAGGCAG AAGTTGACCC ACCTTCAGAC TTGAATTTTA AAATTATAGA TGAAAATACT 
GTTCATATGT CATGGGCAAA ACCAGTTGAT CCAATTGTGG GTTACAGAAT AACGGTGGAC 
CCTACAACGG ATGGGCCTAC TAAAGAATTT ACCCTTTCAG CTAGTACCAC TGAAACTTTA 
TTGTCAGAAC TTGTACCTGA AACAGAGTAT GTGGTGACAA TAACTTCATA TGATGAAGTA 
GAAGAAAGTG TACCAGTTAT AGGACAACTA ACAATTCAAA CAGGTAGTTC GACAAAGCCA 
GTGGAGAAGA AACCTGGAAA AACCGAGATA CAAAAATGCT CTGTCAGTGC CTGGACTGAT 
TTGGTTTTCC TCGTGGATGG CTCTTGGAGT GTGGGAAGAA ATAATTTCAA GTACATTTTA 
GACTTCATTG CTGCTCTTGT GTCTGCTTTT GACATTGGGG AAGAGAAGAC AAGAGTTGGA 
GTTGTTCAAT ACAGCTCTGA TACCAGGACT GAATTTAACT TAAATCAGTA CTACCAAAGG 
GATGAACTTC TTGCTGCAAT AAAAAAAATT CCATATAAAG GTGGCAACAC AATGACAGGG 
GATGCCATTG ATTATTTAGT TAAAAATACT TTCACGGAAT CTGCTGGGGC AAGAGTTGGC 
TTTCCTAAAG TGGCAATTAT TATTACGGAT GGAAAATCCC AGGATGAAGT GGAAATTCCA 
GCAAGAGAGC TTCGTAATGT TGGAGTTGAA GTTTTCTCCT TGGGCATTAA AGCTGCAGAT 
GCAAAAGAAC TCAAACAAAT TGCCTCCACA CCTTCACTGA ACCATGTTTT CAATGTGGCC 
AACTTTGATG CAATTGTGGA TATTCAGAAT GAGATCATCT CCCAGGTGTG CTCAGGTGTT 
GATGAACAAC TTGGTGAATT GGTTAGTGGA GAAGAAGTTG TTGAGCCTCC TTCAAATTTG 
ATTGCCATGG AAGTCTCTTC AAAATATGTT AAGCTAAATT GGAATCCATC TCCTAGTCCA 
GTGACTGGCT ACAAAGTCAT CCTCACACCA ATGACTGCAG GAAGCCGACA GCACGCTCTG 
AGTGTGGGGC CTCAGACAAC CACGCTCAGT GTTCGCGACC TCTCAGCAGA CACAGAATAC 
CAGATCAGTG TTTCCGCCAT GAAGGGAATG ACATCCAGTG AACCCATTTC AATAATGGAG 
AAGACTCAGC CAATGAAAGT TCAAGTGGAA TGCTCACGTG GTGTGGATAT AAAAGCCGAT 
ATTGTGTTTT TGGTTGATGG CTCCTATAGC ATTGGGATTG CAAACTTTGT TAAAGTTAGA 
GCCTTTTTGG AAGTTCTTGT AAAAAGTTTT GAAATTTCAC CAAATAGGGT CCAGATTAGT 
CTTGTGCAAT ACAGCCGGGA TCCTCATACT GAGTTCACTT TGAAAAAATT CACCAAAGTT 
GAAGATATAA TTGAAGCAAT AAACACCTTC CCTTACAGAG GAGGATCTAC AAATACTGGC 
AAAGCAATGA CTTATGTCAG AGAGAAAATA TTTGTGCCTA GCAAGGGATC AAGAAGCAAT 
GTGCCAAAGG TCATGATTCT TATCACGGAT GGGAAATCAT CAGATGCTTT CAGAGATCCT 
GCGATAAAAC TGAGGAATTC AGATGTTGAA ATCTTTGCAG TTGGTGTGAA GGATGCCGTT 
CGCTCAGAAT TGGAAGCTAT TGCCTCTCCT CCTGCAGAGA CCCATGTGTT CACAGTGGAA 
GATTTTGATG CTTTTCAGAG GATATCTTTT GAACTCACAC AGTCTATCTG CCTTAGAATT 
GAGCAAGAAT TGGCAGCTAT AAAGAAGAAA GCTTACGTCC CTCCAAAGGA TCTTAGTTTT 
TCAGAAGTGA CTTCTTATGG TTTCAAAACC AACTGGTCTC CAGCTGGAGA AAATGTTTTT 
TCATATCACA TCACCTACAA GGAAGCGGCT GGGGATGATG AGGTCACTGT GGTGGAGCCA 
GCATCGAGCA CCAGTGTTGT TCTCAGCAGC CTGAAGCCAG AGACCTTGTA TTTGGTCAAT 
GTGACTGCGG AGTATGAGGA TGGCTTCAGC ATTCCCTTAG CTGGAGAGGA GACCACCGAA 
GAAGTAAAAG GAGCACCTCG AAACCTAAAG GTGACAGATG AGACTACAGA TAGTTTCAAA 
ATTACTTGGA CTCAAGCTCC AGGGAGAGTT TTAAGATATC GAATTATATA TAGACCAGTT 
GCTGGTGGAG AGAGCAGAGA AGTTACCACC CCACCCAATC AGAGGAGGAG AACACTGGAG 
AACTTGATTC CAGACACGAA ATATGAAGTA TCTGTAATTC CTGAATATTT CTCAGGACCT 
GGTACTCCAT TAACTGGAAA TGCAGCCACT GAAGAAGTTA GAGGGAATCC AAGAGACTTA 
AGAGTTTCTG ACCCTACGAC GTCTACTATG AAATTATCTT GGAGTGGGGC ACCAGGAAAA 
GTGAAACAGT ATCTCGTCAC ATATACCCCA GTGGCAGGGG GTGAAACTCA AGAGGTCACT 
GTGAGGGGAG ATACAACCAA TACGGTGCTG CAGGGATTGA AGGAAGGGAC ACAATACGCC 
TTATCTGTGA CAGCCTTGTA TGCGTCTGGG GCTGGAGACG CCCTCTTTGG TGAAGGAACA 
ACACTTGAAG AACGTGGTTC TCCTCAAGAT TTAGTTACTA AAGACATCAC TGACACATCA 
ATTGGGGCTT ATTGGACATC TGCTCCAGGA ATGGTTCGCG GTTACAGGGT CTCATGGAAA 
TCACTTTATG ATGATGTTGA CACTGGAGAG AAAAATCTGC CTGAAGATGC AATTCATACG 
ATGATAGAAA ATCTGCAGCC AGAGACCAAA TACAGAATTT CAGTATTTGC CACTTACAGC 
AGTGGAGAAG GAGAACCTTT GACTGGAGAT GCCACAACTG AATTATCTCA AGATTCCAAA 
ACCCTGAAAG TAGATGAAGA AACAGAAAAC ACAATGAGAG TTACATGGAA ACCAGCACCA 
GGGAAAGTCG TCAACTACCG TGTTGTCTAT CGCCCTCATG GGAGAGGGAA GCAAATGGTT 
GCTAAGGTGC CCCCCACAGT CACTTCGACA GTGTTAAAGC GACTTCAGCC ACAGACCACA 
TATGACATCA CAGTTCTTCC TATTTACAAG ATGGGAGAAG GAAAGCTTAG GCAAGGATCA 
GGAACAACAG CTTCTCGGTT TAAGTCTCCT AGAAACCTCA AAACATCTGA CCCAACCATG 
TCAAGCTTCC GAGTGACTTG GGAGCCTGCC CCTGGGGAAG TGAAGGGTTA TAAAGTCACA 
TTCCACCCTA CGGGGGATGA CAGAAGACTG GGGGAGTTAG TGGTTGGACC CTATGACAAC 
ACAGTTGTTT TGGAGGAACT TAGGGCTGGT ACCACCTATA AAGTAAATGT TTTTGGAATG 
TTTGATGGAG GAGAAAGCTC ACCACTTGTT GGACAAGAAA TGACAACCCT TTCCGACACA 
ACTGTTATGC CAATTTTATC TTCTGGGATG GAGTGTCTCA CCAGAGCTGA GGCAGACATT 
GTGTTGCTGG TGGATGGATC ATGGAGCATC GGCCGGGCAA ATTTTAGAAC CGTGAGGAGT 
TTCATTTCTC GTATTGTGGA AGTCTTTGAC ATTGGCCCCA AAAGAGTACA AATTGCTCTT 
GCTCAGTATA GTGGGGATCC CAGAACAGAG TGGCAGTTAA ATGCACACAG AGACAAGAAG 
AGCTTGTTGC AAGCTGTGGC AAACTTGCCG TACAAAGGAG GCAATACTCT CACAGGCATG 
GCTTTGAATT TCATTCGCCA ACAGAACTTC AGGACCCAAG CTGGCATGAG ACCTCGAGCT 
CGAAAAATTG GTGTGCTCAT TACTGATGGA AAATCACAAG ACGATGTTGA AGCACCTTCA 
AAGAAACTCA AGGATGAGGG AGTGGAGCTG TTTGCTATTG GTATTAAAAA TGCTGATGAA 
GTCGAATTAA AGATGATTGC AACTGATCCT GATGATACCC ATGCATACAA TGTGGCAGAT 
TTTGAGTCAC TCTCCAGGAT AGTGGATGAT CTCACCATTA ATTTGTGTAA CAGTGTCAAA 
GGTCCAGGTG ATTTGGAAGC ACCTTCTAAC TTAGTTATTT CTGAGCGAAC CCATCGTTCT 
TTTAGAGTGA GCTGGACACC ACCTTCTGAC AGTGTGGATC GATATAAGGT GGAATACTAT 
CCAGTTTCTG GAGGGAAACG TCAAGAATTT TATGTGAGTC GAATGGAAAC TAGCACAGTG 
CTGAAAGATC TGAAACCTGA AACTGAATAT GTTGTCAATG TGTATTCTGT GGTAGAAGAT 
GAATATAGTG AGCCTCTGAA GGGGACAGAA AAAACCTTGC CAGTGCCTGT AGTCAGCCTG 
AATATTTATG ATGTTGGCCC TACCACCATG CATGTGCAGT GGCAGCCTGT GGGAGGAGCT 
ACTGGCTACA TCTTGTCATA CAAACCTGTT AAGGACACAG AGCCAACAAG ACCCAAAGAG 
GTGCGTTTGG GGCCAACAGT GAATGACATG CAGCTGACTG ACCTTGTTCC CAACACGGAG 
TATGCAGTCA CAGTCCAGGC TGTCCTGCAC GACCTCACTA GTGAACCTGT CACTGTTCGG 
GAAGTCACCT TGCCTTTACC CAGACCTCAG GATCTGAAAC TCAGAGATGT GACTCACAGC 
ACTATGAATG TCTTTTGGGA ACCTGTGCCT GGAAAAGTGC GTAAATATAT TGTTCGATAC 
AAAACACCAG AAGAGGATGT CAAAGAGGTA GAGGTGGACA GATCAGAGAC CAGCACTTCC 
CTCAAAGACC TCTTCTCACA GACCTTGTAC ACAGTCAGCG TTTCTGCAGT ACATGACGAG 
GGGGAGTCTC CTCCAGTGAC TGCTCAAGAA ACTACCCGAC CCGTGCCAGC CCCAACAAAC 
TTAAAGATTA CTGAAGTAAC ATCAGAGGGT TTCAGAGGGA CTTGGGATCA TGGAGCTTCA 
GATGTGTCTC TCTACAGAAT AACTTGGGCA CCTTTTGGAA GCTCAGATAA GATGGAGACC 
ATCTTAAATG GAGATGAAAA CACTTTGGTG TTCGAAAACC TGAACCCCAA CACCATCTAT 
GAAGTTTCCA TTACTGCCAT CTATCCTGAT GAGTCAGAAA GTGATGACCT GATTGGCAGT 
GAGCGCACAC TGCCTATCTT AACAACACAA GCTCCCAAAA GTGGCCCACG AAACCTTCAA 
GTGTACAATG CAACATCTAA CAGCCTGACT GTTAAGTGGG ATCCTGCTAG TGGTCGTGTG 
CAGAAATATA GGATCACTTA TCAGCCTTCC ACAGGGGAAG GCAATGAGCA AACGACCACA 
ATAGGAGGAC GGCAGAACAG TGTGGTCCTG CAGAAACTGA AGCCAGACAC TCCTTACACT 
ATCACCGTAT CCTCTCTGTA TCCTGATGGT GAAGGAGGTC GGATGACGGG AAGAGGCAAG 
ACCAAACCTC TAAACACTGT AAGGAACCTG AGAGTGTATG ACCCTTCTAC CAGCACCTTG 
AATGTCCGCT GGGACCATGC AGAGGGAAAT CCTCGTCAGT ACAAGCTCTT CTATGCACCA 
GCAGCAGGTG GTCCAGAGGA ACTGGTACCA ATCCCCGGGA ATACCAATTA TGCCATTCTT 
AGGAATCTGC AGCCAGATAC CTCATACACT GTGACTGTAG TTCCCGTTTA TACTGAAGGT 
GATGGGGGAC GCACATCAGA TACTGGAAGG ACATTGATGA GAGGACTGGC AAGAAATGTC 
CAAGTATACA ATCCTACACC TAACAGCCTC GATGTTCGCT GGGACCCTGC TCCAGGACCT 
GTGCTGCAAT ATCGCGTTGT GTATTCTCCT GTGGATGGCA CAAGACCCTC AGAATCTATA 
GTAGTGCCAG GAAACACGCG CATGGTGCAT CTGGAGCGGC TGATTCCGGA CACACTCTAT 
TCCGTGAACC TTGTGGCTCT GTACTCGGAT GGAGAGGGAA ATCCCAGCCC TGCCCAGGGC 
CGAACGCTAC CACGCAGTGG ACCAAGGAAC CTGAGAGTCT TTGGTGAAAC AACCAATAGC 
CTCTCGGTAG CCTGGGATCA TGCTGATGGG CCAGTTCAGC AGTACAGGAT CATCTATTCT 
CCCACTGTTG GTGATCCAAT TGATGAATAT ACCACAGTCC CAGGCAGAAG AAACAATGTA 
ATACTGCAGC CCCTGCAACC TGACACTCCA TATAAAATTA CTGTTATTGC TGTTTATGAA 
GATGGAGATG GTGGCCATCT AACAGGAAAT GGAAGAACTG TGGGACTCCT TCCTCCTCAG 
AACATACACA TCTCTGACGA ATGGTATACA AGATTCAGGG TGTCCTGGGA TCCTTCACCT 
TCTCCAGTTC TTGGATATAA AATAGTATAT AAGCCAGTGG GTTCCAATGA GCCCATGGAA 
GCCTTTGTTG GAGAAATGAC ATCATATACC TTACACAATC TCAATCCCAG CACCACCTAC 
GATGTGAATG TTTATGCTCA ATATGATTCT GGACTCAGTG TCCCCTTGAC AGATCAAGGC 
ACTACATTAT ATTTAAATGT AACAGATCTG AAAACTTACC AGATTGGGTG GGATACATTC 
TGTGTCAAAT GGTCACCTCA CCGGGCAGCC ACCTCCTACA GGCTAAAACT AAGCCCTGCA 
GATGGAACAA GGGGACAAGA AATTACAGTG CGTGGATCAG AAACCAGTCA CTGCTTCACT 
GGCCTTTCAC CAGACACTGA TTATGGTGTC ACTGTTTTTG TGCAGACACC AAATCTCGAG 
GGACCAGGAG TCTCTGTTAA AGAACATACC ACTGTGAAAC CAACAGAAGC CCCTACAGAG 
CCACCCACAC CTCCTCCCCC TCCCACCATT CCACCAGCCC GGGATGTATG CAAAGGGGCC 
AAGGCAGATA TTGTGTTCTT GACTGATGCC TCCTGGAGCA TTGGGGACGA TAATTTTAAC 
AAAGTTGTAA AATTCATCTT CAATACTGTG GGAGGCTTTG ATGAAATCAG TCCTGCTGGG 
ATTCAGGTTT CATTTGTGCA ATACAGCGAT GAGGTCAAGT CTGAGTTCAA GCTGAACACG 
TACAATGACA AGGCCCTAGC CCTTGGGGCC CTCCAGAATA TTAGGTACAG AGGAGGAAAC 
ACAAGAACAG GCAAGGCCCT CACGTTTATC AAGGAGAAAG TCTTGACTTG GGAGAGCGGC 
ATGAGGAAGA ATGTCCCTAA GGTGTTGGTT GTGGTCACGG ACGGTCGGTC CCAGGATGAG 
GTCAAGAAGG CGGCTTTGGT CATCCAGCAG TCAGGGTTCA GTGTCTTTGT AGTTGGTGTG 
GCTGATGTCG ACTACAATGA GCTTGCCAAC ATTGCCAGCA AACCAAGTGA ACGGCACGTG 
TTCATTGTGG ACGACTTTGA ATCTTTTGAG AAGATCGAAG ACAATCTTAT TACATTTGTT 
TGTGAAACTG CCACTTCAAG TTGTCCTCTC ATTTATTTGG ATGGCTACAC CTCACCAGGT 
TTTAAAATGC TTGAAGCATA CAACCTGACA GAAAAGAATT TTGCTTCTGT ACAAGGAGTA 
TCTTTGGAGT CAGGGTCTTT CCCCAGCTAC TCAGCATACA GGATTCAGAA GAATGCGTTT 
GTGAATCAGC CTACAGCAGA CCTACACCCA AATGGACTCC CTCCTTCATA CACGATTATA 
TTATTATTCA GACTTCTCCC AGAAACTCCC AGTGACCCTT TTGCAATTTG GCAAATCACA 
GACAGAGACT ACAAACCACA AGTTGGAGTG ATTGCAGATC CTTCTAGCAA GACGTTATCA 
TTCTTTAACA AGGATACAAG AGGCGAGGTG CAAACTGTTA CATTTGACAC AGAAGAAGTA 
AAGACATTAT TTTATGGAAG TTTTCACAAG GTTCATATTG TAGTGACCTC AAAAAGTGTT 
AAGATTTACA TTGACTGCTA TGAAATTATA GAAAAAGACA TCAAGGAAGC TGGAAATATA 
ACAACTGATG GTTATGAAAT TCTTGGAAAA CTCCTTAAAG GGGAAAGGAA ATCAGCCGCA 
TTCCAAATCC AGAGTTTTGA CATTGTCTGC AGTCCAGTGT GGACCAGTAG AGACAGATGC 
TGTGATATTC CCTCTAGGAG AGATGAGGGA AAATGCCCTG CTTTTCCAAA TTCTTGCACA 
TGTACACAGG ACAGCGTTGG ACCTCCAGGA CCTCCAGGCC CTGCAGGAGG ACCTGGTGCT 
AAAGGTCCCA GAGGTGAAAG AGGTATCAGT GGGGCAATTG GGCCCCCTGG TCCTCGTGGA 
GACATAGGTC CTCCAGGCCC CCAGGGTCCT CCAGGCCCTC AGGGACCCAA TGGACTCTCT 
ATTCCGGGAG AGCAAGGTCG CCAAGGGATG AAAGGTGATG CTGGAGAGCC AGGACTTCCA 
GGCCGAACAG GAACCCCAGG ATTACCTGGC CCACCAGGAC CAATGGGACC TCCAGGAGAC 
AGAGGCTTCA CTGGAAAAGA CGGTGCAATG GGACCCAGGG GCCCACCAGG GCCGCCGGGA 
AGCCCAGGCT CCCCAGGAGT CACAGGACCA AGTGGGAAGC CAGGAAAACC TGGAGATCAT 
GGCAGACCAG GTCCATCTGG GTTGAAAGGA GAAAAAGGTG ATAGGGGAGA CATTGCTTCC 
CAGAACATGA TGCGAGCAGT TGCAAGACAA GTCTGTGAAC AATTGATAAG TGGTCAGATG 
AACAGATTCA ATCAGATGCT GAATCAGATT CCAAATGATT ACCAGTCCAG TCGCAACCAG 
CCAGGCCCGC CGGGTCCACC GGGACCTCCT GGTAGCGCAG GAGCCAGAGG AGAACCTGGG 
CCTGGGGGGC GGCCAGGCTT CCCGGGCACA CCAGGGATGC AGGGACCCCC TGGGGAACGA 
GGTTTGCCAG GAGAGAAAGG TGAAAGGGGT ACTGGATCTT CAGGACCTCG GGGGCTGCCT 
GGCCCCCCAG GTCCACAAGG AGAATCCAGA ACAGGTCCAC CAGGGTCCAC AGGTTCAAGA 
GGTCCCCCTG GCCCCCCTGG CCGTCCTGGA AACTCAGGTA TCCGAGGACC CCCAGGTCCT 
CCTGGATACT GTGATTCTTC TCAGTGTGCC AGCATCCCAT ACAACGGGCA AGGCTATCCA 
GGTTCCGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL12A1 ( NM_080645.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_542376.2 Homo sapiens collagen type XII alpha 1 chain (COL12A1), transcript variant short, mRNA.

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CloneID	OHu05790
Clone ID Related Accession (Same CDS sequence)	NM_080645.2 , NM_080645.3
Accession Version	NM_080645.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-12
Organism	Homo sapiens(human)
Product	collagen alpha-1(XII) chain short isoform precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA661773.1, U73778.1, AL096771.11 and AI752603.1. On Apr 21, 2006 this sequence version replaced NM_080645.1. Transcript Variant: This variant (short) lacks multiple exons in the 5' coding region compared to the long variant. The encoded isoform (short) is shorter but has identical N-and C-termini compared to the long isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC146868.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGAGTA GGCTTCCCCC AGCGCTTGCC GCCCTGGGCG CGGCCCTGCT CCTGTCTTCC 
ATTGAGGCAG AAGGGATGGA GTGTCTCACC AGAGCTGAGG CAGACATTGT GTTGCTGGTG 
GATGGATCAT GGAGCATCGG CCGGGCAAAT TTTAGAACCG TGAGGAGTTT CATTTCTCGT 
ATTGTGGAAG TCTTTGACAT TGGCCCCAAA AGAGTACAAA TTGCTCTTGC TCAGTATAGT 
GGGGATCCCA GAACAGAGTG GCAGTTAAAT GCACACAGAG ACAAGAAGAG CTTGTTGCAA 
GCTGTGGCAA ACTTGCCGTA CAAAGGAGGC AATACTCTCA CAGGCATGGC TTTGAATTTC 
ATTCGCCAAC AGAACTTCAG GACCCAAGCT GGCATGAGAC CTCGAGCTCG AAAAATTGGT 
GTGCTCATTA CTGATGGAAA ATCACAAGAC GATGTTGAAG CACCTTCAAA GAAACTCAAG 
GATGAGGGAG TGGAGCTGTT TGCTATTGGT ATTAAAAATG CTGATGAAGT CGAATTAAAG 
ATGATTGCAA CTGATCCTGA TGATACCCAT GCATACAATG TGGCAGATTT TGAGTCACTC 
TCCAGGATAG TGGATGATCT CACCATTAAT TTGTGTAACA GTGTCAAAGG TCCAGGTGAT 
TTGGAAGCAC CTTCTAACTT AGTTATTTCT GAGCGAACCC ATCGTTCTTT TAGAGTGAGC 
TGGACACCAC CTTCTGACAG TGTGGATCGA TATAAGGTGG AATACTATCC AGTTTCTGGA 
GGGAAACGTC AAGAATTTTA TGTGAGTCGA ATGGAAACTA GCACAGTGCT GAAAGATCTG 
AAACCTGAAA CTGAATATGT TGTCAATGTG TATTCTGTGG TAGAAGATGA ATATAGTGAG 
CCTCTGAAGG GGACAGAAAA AACCTTGCCA GTGCCTGTAG TCAGCCTGAA TATTTATGAT 
GTTGGCCCTA CCACCATGCA TGTGCAGTGG CAGCCTGTGG GAGGAGCTAC TGGCTACATC 
TTGTCATACA AACCTGTTAA GGACACAGAG CCAACAAGAC CCAAAGAGGT GCGTTTGGGG 
CCAACAGTGA ATGACATGCA GCTGACTGAC CTTGTTCCCA ACACGGAGTA TGCAGTCACA 
GTCCAGGCTG TCCTGCACGA CCTCACTAGT GAACCTGTCA CTGTTCGGGA AGTCACCTTG 
CCTTTACCCA GACCTCAGGA TCTGAAACTC AGAGATGTGA CTCACAGCAC TATGAATGTC 
TTTTGGGAAC CTGTGCCTGG AAAAGTGCGT AAATATATTG TTCGATACAA AACACCAGAA 
GAGGATGTCA AAGAGGTAGA GGTGGACAGA TCAGAGACCA GCACTTCCCT CAAAGACCTC 
TTCTCACAGA CCTTGTACAC AGTCAGCGTT TCTGCAGTAC ATGACGAGGG GGAGTCTCCT 
CCAGTGACTG CTCAAGAAAC TACCCGACCC GTGCCAGCCC CAACAAACTT AAAGATTACT 
GAAGTAACAT CAGAGGGTTT CAGAGGGACT TGGGATCATG GAGCTTCAGA TGTGTCTCTC 
TACAGAATAA CTTGGGCACC TTTTGGAAGC TCAGATAAGA TGGAGACCAT CTTAAATGGA 
GATGAAAACA CTTTGGTGTT CGAAAACCTG AACCCCAACA CCATCTATGA AGTTTCCATT 
ACTGCCATCT ATCCTGATGA GTCAGAAAGT GATGACCTGA TTGGCAGTGA GCGCACACTG 
CCTATCTTAA CAACACAAGC TCCCAAAAGT GGCCCACGAA ACCTTCAAGT GTACAATGCA 
ACATCTAACA GCCTGACTGT TAAGTGGGAT CCTGCTAGTG GTCGTGTGCA GAAATATAGG 
ATCACTTATC AGCCTTCCAC AGGGGAAGGC AATGAGCAAA CGACCACAAT AGGAGGACGG 
CAGAACAGTG TGGTCCTGCA GAAACTGAAG CCAGACACTC CTTACACTAT CACCGTATCC 
TCTCTGTATC CTGATGGTGA AGGAGGTCGG ATGACGGGAA GAGGCAAGAC CAAACCTCTA 
AACACTGTAA GGAACCTGAG AGTGTATGAC CCTTCTACCA GCACCTTGAA TGTCCGCTGG 
GACCATGCAG AGGGAAATCC TCGTCAGTAC AAGCTCTTCT ATGCACCAGC AGCAGGTGGT 
CCAGAGGAAC TGGTACCAAT CCCCGGGAAT ACCAATTATG CCATTCTTAG GAATCTGCAG 
CCAGATACCT CATACACTGT GACTGTAGTT CCCGTTTATA CTGAAGGTGA TGGGGGACGC 
ACATCAGATA CTGGAAGGAC ATTGATGAGA GGACTGGCAA GAAATGTCCA AGTATACAAT 
CCTACACCTA ACAGCCTCGA TGTTCGCTGG GACCCTGCTC CAGGACCTGT GCTGCAATAT 
CGCGTTGTGT ATTCTCCTGT GGATGGCACA AGACCCTCAG AATCTATAGT AGTGCCAGGA 
AACACGCGCA TGGTGCATCT GGAGCGGCTG ATTCCGGACA CACTCTATTC CGTGAACCTT 
GTGGCTCTGT ACTCGGATGG AGAGGGAAAT CCCAGCCCTG CCCAGGGCCG AACGCTACCA 
CGCAGTGGAC CAAGGAACCT GAGAGTCTTT GGTGAAACAA CCAATAGCCT CTCGGTAGCC 
TGGGATCATG CTGATGGGCC AGTTCAGCAG TACAGGATCA TCTATTCTCC CACTGTTGGT 
GATCCAATTG ATGAATATAC CACAGTCCCA GGCAGAAGAA ACAATGTAAT ACTGCAGCCC 
CTGCAACCTG ACACTCCATA TAAAATTACT GTTATTGCTG TTTATGAAGA TGGAGATGGT 
GGCCATCTAA CAGGAAATGG AAGAACTGTG GGACTCCTTC CTCCTCAGAA CATACACATC 
TCTGACGAAT GGTATACAAG ATTCAGGGTG TCCTGGGATC CTTCACCTTC TCCAGTTCTT 
GGATATAAAA TAGTATATAA GCCAGTGGGT TCCAATGAGC CCATGGAAGC CTTTGTTGGA 
GAAATGACAT CATATACCTT ACACAATCTC AATCCCAGCA CCACCTACGA TGTGAATGTT 
TATGCTCAAT ATGATTCTGG ACTCAGTGTC CCCTTGACAG ATCAAGGCAC TACATTATAT 
TTAAATGTAA CAGATCTGAA AACTTACCAG ATTGGGTGGG ATACATTCTG TGTCAAATGG 
TCACCTCACC GGGCAGCCAC CTCCTACAGG CTAAAACTAA GCCCTGCAGA TGGAACAAGG 
GGACAAGAAA TTACAGTGCG TGGATCAGAA ACCAGTCACT GCTTCACTGG CCTTTCACCA 
GACACTGATT ATGGTGTCAC TGTTTTTGTG CAGACACCAA ATCTCGAGGG ACCAGGAGTC 
TCTGTTAAAG AACATACCAC TGTGAAACCA ACAGAAGCCC CTACAGAGCC ACCCACACCT 
CCTCCCCCTC CCACCATTCC ACCAGCCCGG GATGTATGCA AAGGGGCCAA GGCAGATATT 
GTGTTCTTGA CTGATGCCTC CTGGAGCATT GGGGACGATA ATTTTAACAA AGTTGTAAAA 
TTCATCTTCA ATACTGTGGG AGGCTTTGAT GAAATCAGTC CTGCTGGGAT TCAGGTTTCA 
TTTGTGCAAT ACAGCGATGA GGTCAAGTCT GAGTTCAAGC TGAACACGTA CAATGACAAG 
GCCCTAGCCC TTGGGGCCCT CCAGAATATT AGGTACAGAG GAGGAAACAC AAGAACAGGC 
AAGGCCCTCA CGTTTATCAA GGAGAAAGTC TTGACTTGGG AGAGCGGCAT GAGGAAGAAT 
GTCCCTAAGG TGTTGGTTGT GGTCACGGAC GGTCGGTCCC AGGATGAGGT CAAGAAGGCG 
GCTTTGGTCA TCCAGCAGTC AGGGTTCAGT GTCTTTGTAG TTGGTGTGGC TGATGTCGAC 
TACAATGAGC TTGCCAACAT TGCCAGCAAA CCAAGTGAAC GGCACGTGTT CATTGTGGAC 
GACTTTGAAT CTTTTGAGAA GATCGAAGAC AATCTTATTA CATTTGTTTG TGAAACTGCC 
ACTTCAAGTT GTCCTCTCAT TTATTTGGAT GGCTACACCT CACCAGGTTT TAAAATGCTT 
GAAGCATACA ACCTGACAGA AAAGAATTTT GCTTCTGTAC AAGGAGTATC TTTGGAGTCA 
GGGTCTTTCC CCAGCTACTC AGCATACAGG ATTCAGAAGA ATGCGTTTGT GAATCAGCCT 
ACAGCAGACC TACACCCAAA TGGACTCCCT CCTTCATACA CGATTATATT ATTATTCAGA 
CTTCTCCCAG AAACTCCCAG TGACCCTTTT GCAATTTGGC AAATCACAGA CAGAGACTAC 
AAACCACAAG TTGGAGTGAT TGCAGATCCT TCTAGCAAGA CGTTATCATT CTTTAACAAG 
GATACAAGAG GCGAGGTGCA AACTGTTACA TTTGACACAG AAGAAGTAAA GACATTATTT 
TATGGAAGTT TTCACAAGGT TCATATTGTA GTGACCTCAA AAAGTGTTAA GATTTACATT 
GACTGCTATG AAATTATAGA AAAAGACATC AAGGAAGCTG GAAATATAAC AACTGATGGT 
TATGAAATTC TTGGAAAACT CCTTAAAGGG GAAAGGAAAT CAGCCGCATT CCAAATCCAG 
AGTTTTGACA TTGTCTGCAG TCCAGTGTGG ACCAGTAGAG ACAGATGCTG TGATATTCCC 
TCTAGGAGAG ATGAGGGAAA ATGCCCTGCT TTTCCAAATT CTTGCACATG TACACAGGAC 
AGCGTTGGAC CTCCAGGACC TCCAGGCCCT GCAGGAGGAC CTGGTGCTAA AGGTCCCAGA 
GGTGAAAGAG GTATCAGTGG GGCAATTGGG CCCCCTGGTC CTCGTGGAGA CATAGGTCCT 
CCAGGCCCCC AGGGTCCTCC AGGCCCTCAG GGACCCAATG GACTCTCTAT TCCGGGAGAG 
CAAGGTCGCC AAGGGATGAA AGGTGATGCT GGAGAGCCAG GACTTCCAGG CCGAACAGGA 
ACCCCAGGAT TACCTGGCCC ACCAGGACCA ATGGGACCTC CAGGAGACAG AGGCTTCACT 
GGAAAAGACG GTGCAATGGG ACCCAGGGGC CCACCAGGGC CGCCGGGAAG CCCAGGCTCC 
CCAGGAGTCA CAGGACCAAG TGGGAAGCCA GGAAAACCTG GAGATCATGG CAGACCAGGT 
CCATCTGGGT TGAAAGGAGA AAAAGGTGAT AGGGGAGACA TTGCTTCCCA GAACATGATG 
CGAGCAGTTG CAAGACAAGT CTGTGAACAA TTGATAAGTG GTCAGATGAA CAGATTCAAT 
CAGATGCTGA ATCAGATTCC AAATGATTAC CAGTCCAGTC GCAACCAGCC AGGCCCGCCG 
GGTCCACCGG GACCTCCTGG TAGCGCAGGA GCCAGAGGAG AACCTGGGCC TGGGGGGCGG 
CCAGGCTTCC CGGGCACACC AGGGATGCAG GGACCCCCTG GGGAACGAGG TTTGCCAGGA 
GAGAAAGGTG AAAGGGGTAC TGGATCTTCA GGACCTCGGG GGCTGCCTGG CCCCCCAGGT 
CCACAAGGAG AATCCAGAAC AGGTCCACCA GGGTCCACAG GTTCAAGAGG TCCCCCTGGC 
CCCCCTGGCC GTCCTGGAAA CTCAGGTATC CGAGGACCCC CAGGTCCTCC TGGATACTGT 
GATTCTTCTC AGTGTGCCAG CATCCCATAC AACGGGCAAG GCTATCCAGG TTCCGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_542376.2
CDS	167..5866
Translation	MRSRLPPALAALGAALLLSSIEAEGMECLTRAEADIVLLVDGSWSIGRANFRTVRSFISRIVEVFDIGPKRVQIALAQYSGDPRTEWQLNAHRDKKSLLQAVANLPYKGGNTLTGMALNFIRQQNFRTQAGMRPRARKIGVLITDGKSQDDVEAPSKKLKDEGVELFAIGIKNADEVELKMIATDPDDTHAYNVADFESLSRIVDDLTINLCNSVKGPGDLEAPSNLVISERTHRSFRVSWTPPSDSVDRYKVEYYPVSGGKRQEFYVSRMETSTVLKDLKPETEYVVNVYSVVEDEYSEPLKGTEKTLPVPVVSLNIYDVGPTTMHVQWQPVGGATGYILSYKPVKDTEPTRPKEVRLGPTVNDMQLTDLVPNTEYAVTVQAVLHDLTSEPVTVREVTLPLPRPQDLKLRDVTHSTMNVFWEPVPGKVRKYIVRYKTPEEDVKEVEVDRSETSTSLKDLFSQTLYTVSVSAVHDEGESPPVTAQETTRPVPAPTNLKITEVTSEGFRGTWDHGASDVSLYRITWAPFGSSDKMETILNGDENTLVFENLNPNTIYEVSITAIYPDESESDDLIGSERTLPILTTQAPKSGPRNLQVYNATSNSLTVKWDPASGRVQKYRITYQPSTGEGNEQTTTIGGRQNSVVLQKLKPDTPYTITVSSLYPDGEGGRMTGRGKTKPLNTVRNLRVYDPSTSTLNVRWDHAEGNPRQYKLFYAPAAGGPEELVPIPGNTNYAILRNLQPDTSYTVTVVPVYTEGDGGRTSDTGRTLMRGLARNVQVYNPTPNSLDVRWDPAPGPVLQYRVVYSPVDGTRPSESIVVPGNTRMVHLERLIPDTLYSVNLVALYSDGEGNPSPAQGRTLPRSGPRNLRVFGETTNSLSVAWDHADGPVQQYRIIYSPTVGDPIDEYTTVPGRRNNVILQPLQPDTPYKITVIAVYEDGDGGHLTGNGRTVGLLPPQNIHISDEWYTRFRVSWDPSPSPVLGYKIVYKPVGSNEPMEAFVGEMTSYTLHNLNPSTTYDVNVYAQYDSGLSVPLTDQGTTLYLNVTDLKTYQIGWDTFCVKWSPHRAATSYRLKLSPADGTRGQEITVRGSETSHCFTGLSPDTDYGVTVFVQTPNLEGPGVSVKEHTTVKPTEAPTEPPTPPPPPTIPPARDVCKGAKADIVFLTDASWSIGDDNFNKVVKFIFNTVGGFDEISPAGIQVSFVQYSDEVKSEFKLNTYNDKALALGALQNIRYRGGNTRTGKALTFIKEKVLTWESGMRKNVPKVLVVVTDGRSQDEVKKAALVIQQSGFSVFVVGVADVDYNELANIASKPSERHVFIVDDFESFEKIEDNLITFVCETATSSCPLIYLDGYTSPGFKMLEAYNLTEKNFASVQGVSLESGSFPSYSAYRIQKNAFVNQPTADLHPNGLPPSYTIILLFRLLPETPSDPFAIWQITDRDYKPQVGVIADPSSKTLSFFNKDTRGEVQTVTFDTEEVKTLFYGSFHKVHIVVTSKSVKIYIDCYEIIEKDIKEAGNITTDGYEILGKLLKGERKSAAFQIQSFDIVCSPVWTSRDRCCDIPSRRDEGKCPAFPNSCTCTQDSVGPPGPPGPAGGPGAKGPRGERGISGAIGPPGPRGDIGPPGPQGPPGPQGPNGLSIPGEQGRQGMKGDAGEPGLPGRTGTPGLPGPPGPMGPPGDRGFTGKDGAMGPRGPPGPPGSPGSPGVTGPSGKPGKPGDHGRPGPSGLKGEKGDRGDIASQNMMRAVARQVCEQLISGQMNRFNQMLNQIPNDYQSSRNQPGPPGPPGPPGSAGARGEPGPGGRPGFPGTPGMQGPPGERGLPGEKGERGTGSSGPRGLPGPPGPQGESRTGPPGSTGSRGPPGPPGRPGNSGIRGPPGPPGYCDSSQCASIPYNGQGYPGSG

Target ORF information:

RefSeq Version	NM_080645.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XII alpha 1 chain (COL12A1), transcript variant short, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080645.2

ORF Insert Sequence:

ATGCGGAGTA GGCTTCCCCC AGCGCTTGCC GCCCTGGGCG CGGCCCTGCT CCTGTCTTCC 
ATTGAGGCAG AAGGGATGGA GTGTCTCACC AGAGCTGAGG CAGACATTGT GTTGCTGGTG 
GATGGATCAT GGAGCATCGG CCGGGCAAAT TTTAGAACCG TGAGGAGTTT CATTTCTCGT 
ATTGTGGAAG TCTTTGACAT TGGCCCCAAA AGAGTACAAA TTGCTCTTGC TCAGTATAGT 
GGGGATCCCA GAACAGAGTG GCAGTTAAAT GCACACAGAG ACAAGAAGAG CTTGTTGCAA 
GCTGTGGCAA ACTTGCCGTA CAAAGGAGGC AATACTCTCA CAGGCATGGC TTTGAATTTC 
ATTCGCCAAC AGAACTTCAG GACCCAAGCT GGCATGAGAC CTCGAGCTCG AAAAATTGGT 
GTGCTCATTA CTGATGGAAA ATCACAAGAC GATGTTGAAG CACCTTCAAA GAAACTCAAG 
GATGAGGGAG TGGAGCTGTT TGCTATTGGT ATTAAAAATG CTGATGAAGT CGAATTAAAG 
ATGATTGCAA CTGATCCTGA TGATACCCAT GCATACAATG TGGCAGATTT TGAGTCACTC 
TCCAGGATAG TGGATGATCT CACCATTAAT TTGTGTAACA GTGTCAAAGG TCCAGGTGAT 
TTGGAAGCAC CTTCTAACTT AGTTATTTCT GAGCGAACCC ATCGTTCTTT TAGAGTGAGC 
TGGACACCAC CTTCTGACAG TGTGGATCGA TATAAGGTGG AATACTATCC AGTTTCTGGA 
GGGAAACGTC AAGAATTTTA TGTGAGTCGA ATGGAAACTA GCACAGTGCT GAAAGATCTG 
AAACCTGAAA CTGAATATGT TGTCAATGTG TATTCTGTGG TAGAAGATGA ATATAGTGAG 
CCTCTGAAGG GGACAGAAAA AACCTTGCCA GTGCCTGTAG TCAGCCTGAA TATTTATGAT 
GTTGGCCCTA CCACCATGCA TGTGCAGTGG CAGCCTGTGG GAGGAGCTAC TGGCTACATC 
TTGTCATACA AACCTGTTAA GGACACAGAG CCAACAAGAC CCAAAGAGGT GCGTTTGGGG 
CCAACAGTGA ATGACATGCA GCTGACTGAC CTTGTTCCCA ACACGGAGTA TGCAGTCACA 
GTCCAGGCTG TCCTGCACGA CCTCACTAGT GAACCTGTCA CTGTTCGGGA AGTCACCTTG 
CCTTTACCCA GACCTCAGGA TCTGAAACTC AGAGATGTGA CTCACAGCAC TATGAATGTC 
TTTTGGGAAC CTGTGCCTGG AAAAGTGCGT AAATATATTG TTCGATACAA AACACCAGAA 
GAGGATGTCA AAGAGGTAGA GGTGGACAGA TCAGAGACCA GCACTTCCCT CAAAGACCTC 
TTCTCACAGA CCTTGTACAC AGTCAGCGTT TCTGCAGTAC ATGACGAGGG GGAGTCTCCT 
CCAGTGACTG CTCAAGAAAC TACCCGACCC GTGCCAGCCC CAACAAACTT AAAGATTACT 
GAAGTAACAT CAGAGGGTTT CAGAGGGACT TGGGATCATG GAGCTTCAGA TGTGTCTCTC 
TACAGAATAA CTTGGGCACC TTTTGGAAGC TCAGATAAGA TGGAGACCAT CTTAAATGGA 
GATGAAAACA CTTTGGTGTT CGAAAACCTG AACCCCAACA CCATCTATGA AGTTTCCATT 
ACTGCCATCT ATCCTGATGA GTCAGAAAGT GATGACCTGA TTGGCAGTGA GCGCACACTG 
CCTATCTTAA CAACACAAGC TCCCAAAAGT GGCCCACGAA ACCTTCAAGT GTACAATGCA 
ACATCTAACA GCCTGACTGT TAAGTGGGAT CCTGCTAGTG GTCGTGTGCA GAAATATAGG 
ATCACTTATC AGCCTTCCAC AGGGGAAGGC AATGAGCAAA CGACCACAAT AGGAGGACGG 
CAGAACAGTG TGGTCCTGCA GAAACTGAAG CCAGACACTC CTTACACTAT CACCGTATCC 
TCTCTGTATC CTGATGGTGA AGGAGGTCGG ATGACGGGAA GAGGCAAGAC CAAACCTCTA 
AACACTGTAA GGAACCTGAG AGTGTATGAC CCTTCTACCA GCACCTTGAA TGTCCGCTGG 
GACCATGCAG AGGGAAATCC TCGTCAGTAC AAGCTCTTCT ATGCACCAGC AGCAGGTGGT 
CCAGAGGAAC TGGTACCAAT CCCCGGGAAT ACCAATTATG CCATTCTTAG GAATCTGCAG 
CCAGATACCT CATACACTGT GACTGTAGTT CCCGTTTATA CTGAAGGTGA TGGGGGACGC 
ACATCAGATA CTGGAAGGAC ATTGATGAGA GGACTGGCAA GAAATGTCCA AGTATACAAT 
CCTACACCTA ACAGCCTCGA TGTTCGCTGG GACCCTGCTC CAGGACCTGT GCTGCAATAT 
CGCGTTGTGT ATTCTCCTGT GGATGGCACA AGACCCTCAG AATCTATAGT AGTGCCAGGA 
AACACGCGCA TGGTGCATCT GGAGCGGCTG ATTCCGGACA CACTCTATTC CGTGAACCTT 
GTGGCTCTGT ACTCGGATGG AGAGGGAAAT CCCAGCCCTG CCCAGGGCCG AACGCTACCA 
CGCAGTGGAC CAAGGAACCT GAGAGTCTTT GGTGAAACAA CCAATAGCCT CTCGGTAGCC 
TGGGATCATG CTGATGGGCC AGTTCAGCAG TACAGGATCA TCTATTCTCC CACTGTTGGT 
GATCCAATTG ATGAATATAC CACAGTCCCA GGCAGAAGAA ACAATGTAAT ACTGCAGCCC 
CTGCAACCTG ACACTCCATA TAAAATTACT GTTATTGCTG TTTATGAAGA TGGAGATGGT 
GGCCATCTAA CAGGAAATGG AAGAACTGTG GGACTCCTTC CTCCTCAGAA CATACACATC 
TCTGACGAAT GGTATACAAG ATTCAGGGTG TCCTGGGATC CTTCACCTTC TCCAGTTCTT 
GGATATAAAA TAGTATATAA GCCAGTGGGT TCCAATGAGC CCATGGAAGC CTTTGTTGGA 
GAAATGACAT CATATACCTT ACACAATCTC AATCCCAGCA CCACCTACGA TGTGAATGTT 
TATGCTCAAT ATGATTCTGG ACTCAGTGTC CCCTTGACAG ATCAAGGCAC TACATTATAT 
TTAAATGTAA CAGATCTGAA AACTTACCAG ATTGGGTGGG ATACATTCTG TGTCAAATGG 
TCACCTCACC GGGCAGCCAC CTCCTACAGG CTAAAACTAA GCCCTGCAGA TGGAACAAGG 
GGACAAGAAA TTACAGTGCG TGGATCAGAA ACCAGTCACT GCTTCACTGG CCTTTCACCA 
GACACTGATT ATGGTGTCAC TGTTTTTGTG CAGACACCAA ATCTCGAGGG ACCAGGAGTC 
TCTGTTAAAG AACATACCAC TGTGAAACCA ACAGAAGCCC CTACAGAGCC ACCCACACCT 
CCTCCCCCTC CCACCATTCC ACCAGCCCGG GATGTATGCA AAGGGGCCAA GGCAGATATT 
GTGTTCTTGA CTGATGCCTC CTGGAGCATT GGGGACGATA ATTTTAACAA AGTTGTAAAA 
TTCATCTTCA ATACTGTGGG AGGCTTTGAT GAAATCAGTC CTGCTGGGAT TCAGGTTTCA 
TTTGTGCAAT ACAGCGATGA GGTCAAGTCT GAGTTCAAGC TGAACACGTA CAATGACAAG 
GCCCTAGCCC TTGGGGCCCT CCAGAATATT AGGTACAGAG GAGGAAACAC AAGAACAGGC 
AAGGCCCTCA CGTTTATCAA GGAGAAAGTC TTGACTTGGG AGAGCGGCAT GAGGAAGAAT 
GTCCCTAAGG TGTTGGTTGT GGTCACGGAC GGTCGGTCCC AGGATGAGGT CAAGAAGGCG 
GCTTTGGTCA TCCAGCAGTC AGGGTTCAGT GTCTTTGTAG TTGGTGTGGC TGATGTCGAC 
TACAATGAGC TTGCCAACAT TGCCAGCAAA CCAAGTGAAC GGCACGTGTT CATTGTGGAC 
GACTTTGAAT CTTTTGAGAA GATCGAAGAC AATCTTATTA CATTTGTTTG TGAAACTGCC 
ACTTCAAGTT GTCCTCTCAT TTATTTGGAT GGCTACACCT CACCAGGTTT TAAAATGCTT 
GAAGCATACA ACCTGACAGA AAAGAATTTT GCTTCTGTAC AAGGAGTATC TTTGGAGTCA 
GGGTCTTTCC CCAGCTACTC AGCATACAGG ATTCAGAAGA ATGCGTTTGT GAATCAGCCT 
ACAGCAGACC TACACCCAAA TGGACTCCCT CCTTCATACA CGATTATATT ATTATTCAGA 
CTTCTCCCAG AAACTCCCAG TGACCCTTTT GCAATTTGGC AAATCACAGA CAGAGACTAC 
AAACCACAAG TTGGAGTGAT TGCAGATCCT TCTAGCAAGA CGTTATCATT CTTTAACAAG 
GATACAAGAG GCGAGGTGCA AACTGTTACA TTTGACACAG AAGAAGTAAA GACATTATTT 
TATGGAAGTT TTCACAAGGT TCATATTGTA GTGACCTCAA AAAGTGTTAA GATTTACATT 
GACTGCTATG AAATTATAGA AAAAGACATC AAGGAAGCTG GAAATATAAC AACTGATGGT 
TATGAAATTC TTGGAAAACT CCTTAAAGGG GAAAGGAAAT CAGCCGCATT CCAAATCCAG 
AGTTTTGACA TTGTCTGCAG TCCAGTGTGG ACCAGTAGAG ACAGATGCTG TGATATTCCC 
TCTAGGAGAG ATGAGGGAAA ATGCCCTGCT TTTCCAAATT CTTGCACATG TACACAGGAC 
AGCGTTGGAC CTCCAGGACC TCCAGGCCCT GCAGGAGGAC CTGGTGCTAA AGGTCCCAGA 
GGTGAAAGAG GTATCAGTGG GGCAATTGGG CCCCCTGGTC CTCGTGGAGA CATAGGTCCT 
CCAGGCCCCC AGGGTCCTCC AGGCCCTCAG GGACCCAATG GACTCTCTAT TCCGGGAGAG 
CAAGGTCGCC AAGGGATGAA AGGTGATGCT GGAGAGCCAG GACTTCCAGG CCGAACAGGA 
ACCCCAGGAT TACCTGGCCC ACCAGGACCA ATGGGACCTC CAGGAGACAG AGGCTTCACT 
GGAAAAGACG GTGCAATGGG ACCCAGGGGC CCACCAGGGC CGCCGGGAAG CCCAGGCTCC 
CCAGGAGTCA CAGGACCAAG TGGGAAGCCA GGAAAACCTG GAGATCATGG CAGACCAGGT 
CCATCTGGGT TGAAAGGAGA AAAAGGTGAT AGGGGAGACA TTGCTTCCCA GAACATGATG 
CGAGCAGTTG CAAGACAAGT CTGTGAACAA TTGATAAGTG GTCAGATGAA CAGATTCAAT 
CAGATGCTGA ATCAGATTCC AAATGATTAC CAGTCCAGTC GCAACCAGCC AGGCCCGCCG 
GGTCCACCGG GACCTCCTGG TAGCGCAGGA GCCAGAGGAG AACCTGGGCC TGGGGGGCGG 
CCAGGCTTCC CGGGCACACC AGGGATGCAG GGACCCCCTG GGGAACGAGG TTTGCCAGGA 
GAGAAAGGTG AAAGGGGTAC TGGATCTTCA GGACCTCGGG GGCTGCCTGG CCCCCCAGGT 
CCACAAGGAG AATCCAGAAC AGGTCCACCA GGGTCCACAG GTTCAAGAGG TCCCCCTGGC 
CCCCCTGGCC GTCCTGGAAA CTCAGGTATC CGAGGACCCC CAGGTCCTCC TGGATACTGT 
GATTCTTCTC AGTGTGCCAG CATCCCATAC AACGGGCAAG GCTATCCAGG TTCCGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL12A1 ( NM_080645.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_542376.2 Homo sapiens collagen type XII alpha 1 chain (COL12A1), transcript variant short, mRNA.

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CloneID	OHu05790
Clone ID Related Accession (Same CDS sequence)	NM_080645.2 , NM_080645.3
Accession Version	NM_080645.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	collagen alpha-1(XII) chain short isoform precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL080250.11, DA661773.1, U73778.1, AL096771.11 and AI752603.1. On May 31, 2019 this sequence version replaced NM_080645.2. Transcript Variant: This variant (short) lacks multiple exons in the 5' coding region compared to the long variant. The encoded isoform (short) is shorter but has identical N-and C-termini compared to the long isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC146868.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGAGTA GGCTTCCCCC AGCGCTTGCC GCCCTGGGCG CGGCCCTGCT CCTGTCTTCC 
ATTGAGGCAG AAGGGATGGA GTGTCTCACC AGAGCTGAGG CAGACATTGT GTTGCTGGTG 
GATGGATCAT GGAGCATCGG CCGGGCAAAT TTTAGAACCG TGAGGAGTTT CATTTCTCGT 
ATTGTGGAAG TCTTTGACAT TGGCCCCAAA AGAGTACAAA TTGCTCTTGC TCAGTATAGT 
GGGGATCCCA GAACAGAGTG GCAGTTAAAT GCACACAGAG ACAAGAAGAG CTTGTTGCAA 
GCTGTGGCAA ACTTGCCGTA CAAAGGAGGC AATACTCTCA CAGGCATGGC TTTGAATTTC 
ATTCGCCAAC AGAACTTCAG GACCCAAGCT GGCATGAGAC CTCGAGCTCG AAAAATTGGT 
GTGCTCATTA CTGATGGAAA ATCACAAGAC GATGTTGAAG CACCTTCAAA GAAACTCAAG 
GATGAGGGAG TGGAGCTGTT TGCTATTGGT ATTAAAAATG CTGATGAAGT CGAATTAAAG 
ATGATTGCAA CTGATCCTGA TGATACCCAT GCATACAATG TGGCAGATTT TGAGTCACTC 
TCCAGGATAG TGGATGATCT CACCATTAAT TTGTGTAACA GTGTCAAAGG TCCAGGTGAT 
TTGGAAGCAC CTTCTAACTT AGTTATTTCT GAGCGAACCC ATCGTTCTTT TAGAGTGAGC 
TGGACACCAC CTTCTGACAG TGTGGATCGA TATAAGGTGG AATACTATCC AGTTTCTGGA 
GGGAAACGTC AAGAATTTTA TGTGAGTCGA ATGGAAACTA GCACAGTGCT GAAAGATCTG 
AAACCTGAAA CTGAATATGT TGTCAATGTG TATTCTGTGG TAGAAGATGA ATATAGTGAG 
CCTCTGAAGG GGACAGAAAA AACCTTGCCA GTGCCTGTAG TCAGCCTGAA TATTTATGAT 
GTTGGCCCTA CCACCATGCA TGTGCAGTGG CAGCCTGTGG GAGGAGCTAC TGGCTACATC 
TTGTCATACA AACCTGTTAA GGACACAGAG CCAACAAGAC CCAAAGAGGT GCGTTTGGGG 
CCAACAGTGA ATGACATGCA GCTGACTGAC CTTGTTCCCA ACACGGAGTA TGCAGTCACA 
GTCCAGGCTG TCCTGCACGA CCTCACTAGT GAACCTGTCA CTGTTCGGGA AGTCACCTTG 
CCTTTACCCA GACCTCAGGA TCTGAAACTC AGAGATGTGA CTCACAGCAC TATGAATGTC 
TTTTGGGAAC CTGTGCCTGG AAAAGTGCGT AAATATATTG TTCGATACAA AACACCAGAA 
GAGGATGTCA AAGAGGTAGA GGTGGACAGA TCAGAGACCA GCACTTCCCT CAAAGACCTC 
TTCTCACAGA CCTTGTACAC AGTCAGCGTT TCTGCAGTAC ATGACGAGGG GGAGTCTCCT 
CCAGTGACTG CTCAAGAAAC TACCCGACCC GTGCCAGCCC CAACAAACTT AAAGATTACT 
GAAGTAACAT CAGAGGGTTT CAGAGGGACT TGGGATCATG GAGCTTCAGA TGTGTCTCTC 
TACAGAATAA CTTGGGCACC TTTTGGAAGC TCAGATAAGA TGGAGACCAT CTTAAATGGA 
GATGAAAACA CTTTGGTGTT CGAAAACCTG AACCCCAACA CCATCTATGA AGTTTCCATT 
ACTGCCATCT ATCCTGATGA GTCAGAAAGT GATGACCTGA TTGGCAGTGA GCGCACACTG 
CCTATCTTAA CAACACAAGC TCCCAAAAGT GGCCCACGAA ACCTTCAAGT GTACAATGCA 
ACATCTAACA GCCTGACTGT TAAGTGGGAT CCTGCTAGTG GTCGTGTGCA GAAATATAGG 
ATCACTTATC AGCCTTCCAC AGGGGAAGGC AATGAGCAAA CGACCACAAT AGGAGGACGG 
CAGAACAGTG TGGTCCTGCA GAAACTGAAG CCAGACACTC CTTACACTAT CACCGTATCC 
TCTCTGTATC CTGATGGTGA AGGAGGTCGG ATGACGGGAA GAGGCAAGAC CAAACCTCTA 
AACACTGTAA GGAACCTGAG AGTGTATGAC CCTTCTACCA GCACCTTGAA TGTCCGCTGG 
GACCATGCAG AGGGAAATCC TCGTCAGTAC AAGCTCTTCT ATGCACCAGC AGCAGGTGGT 
CCAGAGGAAC TGGTACCAAT CCCCGGGAAT ACCAATTATG CCATTCTTAG GAATCTGCAG 
CCAGATACCT CATACACTGT GACTGTAGTT CCCGTTTATA CTGAAGGTGA TGGGGGACGC 
ACATCAGATA CTGGAAGGAC ATTGATGAGA GGACTGGCAA GAAATGTCCA AGTATACAAT 
CCTACACCTA ACAGCCTCGA TGTTCGCTGG GACCCTGCTC CAGGACCTGT GCTGCAATAT 
CGCGTTGTGT ATTCTCCTGT GGATGGCACA AGACCCTCAG AATCTATAGT AGTGCCAGGA 
AACACGCGCA TGGTGCATCT GGAGCGGCTG ATTCCGGACA CACTCTATTC CGTGAACCTT 
GTGGCTCTGT ACTCGGATGG AGAGGGAAAT CCCAGCCCTG CCCAGGGCCG AACGCTACCA 
CGCAGTGGAC CAAGGAACCT GAGAGTCTTT GGTGAAACAA CCAATAGCCT CTCGGTAGCC 
TGGGATCATG CTGATGGGCC AGTTCAGCAG TACAGGATCA TCTATTCTCC CACTGTTGGT 
GATCCAATTG ATGAATATAC CACAGTCCCA GGCAGAAGAA ACAATGTAAT ACTGCAGCCC 
CTGCAACCTG ACACTCCATA TAAAATTACT GTTATTGCTG TTTATGAAGA TGGAGATGGT 
GGCCATCTAA CAGGAAATGG AAGAACTGTG GGACTCCTTC CTCCTCAGAA CATACACATC 
TCTGACGAAT GGTATACAAG ATTCAGGGTG TCCTGGGATC CTTCACCTTC TCCAGTTCTT 
GGATATAAAA TAGTATATAA GCCAGTGGGT TCCAATGAGC CCATGGAAGC CTTTGTTGGA 
GAAATGACAT CATATACCTT ACACAATCTC AATCCCAGCA CCACCTACGA TGTGAATGTT 
TATGCTCAAT ATGATTCTGG ACTCAGTGTC CCCTTGACAG ATCAAGGCAC TACATTATAT 
TTAAATGTAA CAGATCTGAA AACTTACCAG ATTGGGTGGG ATACATTCTG TGTCAAATGG 
TCACCTCACC GGGCAGCCAC CTCCTACAGG CTAAAACTAA GCCCTGCAGA TGGAACAAGG 
GGACAAGAAA TTACAGTGCG TGGATCAGAA ACCAGTCACT GCTTCACTGG CCTTTCACCA 
GACACTGATT ATGGTGTCAC TGTTTTTGTG CAGACACCAA ATCTCGAGGG ACCAGGAGTC 
TCTGTTAAAG AACATACCAC TGTGAAACCA ACAGAAGCCC CTACAGAGCC ACCCACACCT 
CCTCCCCCTC CCACCATTCC ACCAGCCCGG GATGTATGCA AAGGGGCCAA GGCAGATATT 
GTGTTCTTGA CTGATGCCTC CTGGAGCATT GGGGACGATA ATTTTAACAA AGTTGTAAAA 
TTCATCTTCA ATACTGTGGG AGGCTTTGAT GAAATCAGTC CTGCTGGGAT TCAGGTTTCA 
TTTGTGCAAT ACAGCGATGA GGTCAAGTCT GAGTTCAAGC TGAACACGTA CAATGACAAG 
GCCCTAGCCC TTGGGGCCCT CCAGAATATT AGGTACAGAG GAGGAAACAC AAGAACAGGC 
AAGGCCCTCA CGTTTATCAA GGAGAAAGTC TTGACTTGGG AGAGCGGCAT GAGGAAGAAT 
GTCCCTAAGG TGTTGGTTGT GGTCACGGAC GGTCGGTCCC AGGATGAGGT CAAGAAGGCG 
GCTTTGGTCA TCCAGCAGTC AGGGTTCAGT GTCTTTGTAG TTGGTGTGGC TGATGTCGAC 
TACAATGAGC TTGCCAACAT TGCCAGCAAA CCAAGTGAAC GGCACGTGTT CATTGTGGAC 
GACTTTGAAT CTTTTGAGAA GATCGAAGAC AATCTTATTA CATTTGTTTG TGAAACTGCC 
ACTTCAAGTT GTCCTCTCAT TTATTTGGAT GGCTACACCT CACCAGGTTT TAAAATGCTT 
GAAGCATACA ACCTGACAGA AAAGAATTTT GCTTCTGTAC AAGGAGTATC TTTGGAGTCA 
GGGTCTTTCC CCAGCTACTC AGCATACAGG ATTCAGAAGA ATGCGTTTGT GAATCAGCCT 
ACAGCAGACC TACACCCAAA TGGACTCCCT CCTTCATACA CGATTATATT ATTATTCAGA 
CTTCTCCCAG AAACTCCCAG TGACCCTTTT GCAATTTGGC AAATCACAGA CAGAGACTAC 
AAACCACAAG TTGGAGTGAT TGCAGATCCT TCTAGCAAGA CGTTATCATT CTTTAACAAG 
GATACAAGAG GCGAGGTGCA AACTGTTACA TTTGACACAG AAGAAGTAAA GACATTATTT 
TATGGAAGTT TTCACAAGGT TCATATTGTA GTGACCTCAA AAAGTGTTAA GATTTACATT 
GACTGCTATG AAATTATAGA AAAAGACATC AAGGAAGCTG GAAATATAAC AACTGATGGT 
TATGAAATTC TTGGAAAACT CCTTAAAGGG GAAAGGAAAT CAGCCGCATT CCAAATCCAG 
AGTTTTGACA TTGTCTGCAG TCCAGTGTGG ACCAGTAGAG ACAGATGCTG TGATATTCCC 
TCTAGGAGAG ATGAGGGAAA ATGCCCTGCT TTTCCAAATT CTTGCACATG TACACAGGAC 
AGCGTTGGAC CTCCAGGACC TCCAGGCCCT GCAGGAGGAC CTGGTGCTAA AGGTCCCAGA 
GGTGAAAGAG GTATCAGTGG GGCAATTGGG CCCCCTGGTC CTCGTGGAGA CATAGGTCCT 
CCAGGCCCCC AGGGTCCTCC AGGCCCTCAG GGACCCAATG GACTCTCTAT TCCGGGAGAG 
CAAGGTCGCC AAGGGATGAA AGGTGATGCT GGAGAGCCAG GACTTCCAGG CCGAACAGGA 
ACCCCAGGAT TACCTGGCCC ACCAGGACCA ATGGGACCTC CAGGAGACAG AGGCTTCACT 
GGAAAAGACG GTGCAATGGG ACCCAGGGGC CCACCAGGGC CGCCGGGAAG CCCAGGCTCC 
CCAGGAGTCA CAGGACCAAG TGGGAAGCCA GGAAAACCTG GAGATCATGG CAGACCAGGT 
CCATCTGGGT TGAAAGGAGA AAAAGGTGAT AGGGGAGACA TTGCTTCCCA GAACATGATG 
CGAGCAGTTG CAAGACAAGT CTGTGAACAA TTGATAAGTG GTCAGATGAA CAGATTCAAT 
CAGATGCTGA ATCAGATTCC AAATGATTAC CAGTCCAGTC GCAACCAGCC AGGCCCGCCG 
GGTCCACCGG GACCTCCTGG TAGCGCAGGA GCCAGAGGAG AACCTGGGCC TGGGGGGCGG 
CCAGGCTTCC CGGGCACACC AGGGATGCAG GGACCCCCTG GGGAACGAGG TTTGCCAGGA 
GAGAAAGGTG AAAGGGGTAC TGGATCTTCA GGACCTCGGG GGCTGCCTGG CCCCCCAGGT 
CCACAAGGAG AATCCAGAAC AGGTCCACCA GGGTCCACAG GTTCAAGAGG TCCCCCTGGC 
CCCCCTGGCC GTCCTGGAAA CTCAGGTATC CGAGGACCCC CAGGTCCTCC TGGATACTGT 
GATTCTTCTC AGTGTGCCAG CATCCCATAC AACGGGCAAG GCTATCCAGG TTCCGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_542376.2
CDS	313..6012
Translation	MRSRLPPALAALGAALLLSSIEAEGMECLTRAEADIVLLVDGSWSIGRANFRTVRSFISRIVEVFDIGPKRVQIALAQYSGDPRTEWQLNAHRDKKSLLQAVANLPYKGGNTLTGMALNFIRQQNFRTQAGMRPRARKIGVLITDGKSQDDVEAPSKKLKDEGVELFAIGIKNADEVELKMIATDPDDTHAYNVADFESLSRIVDDLTINLCNSVKGPGDLEAPSNLVISERTHRSFRVSWTPPSDSVDRYKVEYYPVSGGKRQEFYVSRMETSTVLKDLKPETEYVVNVYSVVEDEYSEPLKGTEKTLPVPVVSLNIYDVGPTTMHVQWQPVGGATGYILSYKPVKDTEPTRPKEVRLGPTVNDMQLTDLVPNTEYAVTVQAVLHDLTSEPVTVREVTLPLPRPQDLKLRDVTHSTMNVFWEPVPGKVRKYIVRYKTPEEDVKEVEVDRSETSTSLKDLFSQTLYTVSVSAVHDEGESPPVTAQETTRPVPAPTNLKITEVTSEGFRGTWDHGASDVSLYRITWAPFGSSDKMETILNGDENTLVFENLNPNTIYEVSITAIYPDESESDDLIGSERTLPILTTQAPKSGPRNLQVYNATSNSLTVKWDPASGRVQKYRITYQPSTGEGNEQTTTIGGRQNSVVLQKLKPDTPYTITVSSLYPDGEGGRMTGRGKTKPLNTVRNLRVYDPSTSTLNVRWDHAEGNPRQYKLFYAPAAGGPEELVPIPGNTNYAILRNLQPDTSYTVTVVPVYTEGDGGRTSDTGRTLMRGLARNVQVYNPTPNSLDVRWDPAPGPVLQYRVVYSPVDGTRPSESIVVPGNTRMVHLERLIPDTLYSVNLVALYSDGEGNPSPAQGRTLPRSGPRNLRVFGETTNSLSVAWDHADGPVQQYRIIYSPTVGDPIDEYTTVPGRRNNVILQPLQPDTPYKITVIAVYEDGDGGHLTGNGRTVGLLPPQNIHISDEWYTRFRVSWDPSPSPVLGYKIVYKPVGSNEPMEAFVGEMTSYTLHNLNPSTTYDVNVYAQYDSGLSVPLTDQGTTLYLNVTDLKTYQIGWDTFCVKWSPHRAATSYRLKLSPADGTRGQEITVRGSETSHCFTGLSPDTDYGVTVFVQTPNLEGPGVSVKEHTTVKPTEAPTEPPTPPPPPTIPPARDVCKGAKADIVFLTDASWSIGDDNFNKVVKFIFNTVGGFDEISPAGIQVSFVQYSDEVKSEFKLNTYNDKALALGALQNIRYRGGNTRTGKALTFIKEKVLTWESGMRKNVPKVLVVVTDGRSQDEVKKAALVIQQSGFSVFVVGVADVDYNELANIASKPSERHVFIVDDFESFEKIEDNLITFVCETATSSCPLIYLDGYTSPGFKMLEAYNLTEKNFASVQGVSLESGSFPSYSAYRIQKNAFVNQPTADLHPNGLPPSYTIILLFRLLPETPSDPFAIWQITDRDYKPQVGVIADPSSKTLSFFNKDTRGEVQTVTFDTEEVKTLFYGSFHKVHIVVTSKSVKIYIDCYEIIEKDIKEAGNITTDGYEILGKLLKGERKSAAFQIQSFDIVCSPVWTSRDRCCDIPSRRDEGKCPAFPNSCTCTQDSVGPPGPPGPAGGPGAKGPRGERGISGAIGPPGPRGDIGPPGPQGPPGPQGPNGLSIPGEQGRQGMKGDAGEPGLPGRTGTPGLPGPPGPMGPPGDRGFTGKDGAMGPRGPPGPPGSPGSPGVTGPSGKPGKPGDHGRPGPSGLKGEKGDRGDIASQNMMRAVARQVCEQLISGQMNRFNQMLNQIPNDYQSSRNQPGPPGPPGPPGSAGARGEPGPGGRPGFPGTPGMQGPPGERGLPGEKGERGTGSSGPRGLPGPPGPQGESRTGPPGSTGSRGPPGPPGRPGNSGIRGPPGPPGYCDSSQCASIPYNGQGYPGSG

Target ORF information:

RefSeq Version	NM_080645.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XII alpha 1 chain (COL12A1), transcript variant short, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080645.3

ORF Insert Sequence:

ATGCGGAGTA GGCTTCCCCC AGCGCTTGCC GCCCTGGGCG CGGCCCTGCT CCTGTCTTCC 
ATTGAGGCAG AAGGGATGGA GTGTCTCACC AGAGCTGAGG CAGACATTGT GTTGCTGGTG 
GATGGATCAT GGAGCATCGG CCGGGCAAAT TTTAGAACCG TGAGGAGTTT CATTTCTCGT 
ATTGTGGAAG TCTTTGACAT TGGCCCCAAA AGAGTACAAA TTGCTCTTGC TCAGTATAGT 
GGGGATCCCA GAACAGAGTG GCAGTTAAAT GCACACAGAG ACAAGAAGAG CTTGTTGCAA 
GCTGTGGCAA ACTTGCCGTA CAAAGGAGGC AATACTCTCA CAGGCATGGC TTTGAATTTC 
ATTCGCCAAC AGAACTTCAG GACCCAAGCT GGCATGAGAC CTCGAGCTCG AAAAATTGGT 
GTGCTCATTA CTGATGGAAA ATCACAAGAC GATGTTGAAG CACCTTCAAA GAAACTCAAG 
GATGAGGGAG TGGAGCTGTT TGCTATTGGT ATTAAAAATG CTGATGAAGT CGAATTAAAG 
ATGATTGCAA CTGATCCTGA TGATACCCAT GCATACAATG TGGCAGATTT TGAGTCACTC 
TCCAGGATAG TGGATGATCT CACCATTAAT TTGTGTAACA GTGTCAAAGG TCCAGGTGAT 
TTGGAAGCAC CTTCTAACTT AGTTATTTCT GAGCGAACCC ATCGTTCTTT TAGAGTGAGC 
TGGACACCAC CTTCTGACAG TGTGGATCGA TATAAGGTGG AATACTATCC AGTTTCTGGA 
GGGAAACGTC AAGAATTTTA TGTGAGTCGA ATGGAAACTA GCACAGTGCT GAAAGATCTG 
AAACCTGAAA CTGAATATGT TGTCAATGTG TATTCTGTGG TAGAAGATGA ATATAGTGAG 
CCTCTGAAGG GGACAGAAAA AACCTTGCCA GTGCCTGTAG TCAGCCTGAA TATTTATGAT 
GTTGGCCCTA CCACCATGCA TGTGCAGTGG CAGCCTGTGG GAGGAGCTAC TGGCTACATC 
TTGTCATACA AACCTGTTAA GGACACAGAG CCAACAAGAC CCAAAGAGGT GCGTTTGGGG 
CCAACAGTGA ATGACATGCA GCTGACTGAC CTTGTTCCCA ACACGGAGTA TGCAGTCACA 
GTCCAGGCTG TCCTGCACGA CCTCACTAGT GAACCTGTCA CTGTTCGGGA AGTCACCTTG 
CCTTTACCCA GACCTCAGGA TCTGAAACTC AGAGATGTGA CTCACAGCAC TATGAATGTC 
TTTTGGGAAC CTGTGCCTGG AAAAGTGCGT AAATATATTG TTCGATACAA AACACCAGAA 
GAGGATGTCA AAGAGGTAGA GGTGGACAGA TCAGAGACCA GCACTTCCCT CAAAGACCTC 
TTCTCACAGA CCTTGTACAC AGTCAGCGTT TCTGCAGTAC ATGACGAGGG GGAGTCTCCT 
CCAGTGACTG CTCAAGAAAC TACCCGACCC GTGCCAGCCC CAACAAACTT AAAGATTACT 
GAAGTAACAT CAGAGGGTTT CAGAGGGACT TGGGATCATG GAGCTTCAGA TGTGTCTCTC 
TACAGAATAA CTTGGGCACC TTTTGGAAGC TCAGATAAGA TGGAGACCAT CTTAAATGGA 
GATGAAAACA CTTTGGTGTT CGAAAACCTG AACCCCAACA CCATCTATGA AGTTTCCATT 
ACTGCCATCT ATCCTGATGA GTCAGAAAGT GATGACCTGA TTGGCAGTGA GCGCACACTG 
CCTATCTTAA CAACACAAGC TCCCAAAAGT GGCCCACGAA ACCTTCAAGT GTACAATGCA 
ACATCTAACA GCCTGACTGT TAAGTGGGAT CCTGCTAGTG GTCGTGTGCA GAAATATAGG 
ATCACTTATC AGCCTTCCAC AGGGGAAGGC AATGAGCAAA CGACCACAAT AGGAGGACGG 
CAGAACAGTG TGGTCCTGCA GAAACTGAAG CCAGACACTC CTTACACTAT CACCGTATCC 
TCTCTGTATC CTGATGGTGA AGGAGGTCGG ATGACGGGAA GAGGCAAGAC CAAACCTCTA 
AACACTGTAA GGAACCTGAG AGTGTATGAC CCTTCTACCA GCACCTTGAA TGTCCGCTGG 
GACCATGCAG AGGGAAATCC TCGTCAGTAC AAGCTCTTCT ATGCACCAGC AGCAGGTGGT 
CCAGAGGAAC TGGTACCAAT CCCCGGGAAT ACCAATTATG CCATTCTTAG GAATCTGCAG 
CCAGATACCT CATACACTGT GACTGTAGTT CCCGTTTATA CTGAAGGTGA TGGGGGACGC 
ACATCAGATA CTGGAAGGAC ATTGATGAGA GGACTGGCAA GAAATGTCCA AGTATACAAT 
CCTACACCTA ACAGCCTCGA TGTTCGCTGG GACCCTGCTC CAGGACCTGT GCTGCAATAT 
CGCGTTGTGT ATTCTCCTGT GGATGGCACA AGACCCTCAG AATCTATAGT AGTGCCAGGA 
AACACGCGCA TGGTGCATCT GGAGCGGCTG ATTCCGGACA CACTCTATTC CGTGAACCTT 
GTGGCTCTGT ACTCGGATGG AGAGGGAAAT CCCAGCCCTG CCCAGGGCCG AACGCTACCA 
CGCAGTGGAC CAAGGAACCT GAGAGTCTTT GGTGAAACAA CCAATAGCCT CTCGGTAGCC 
TGGGATCATG CTGATGGGCC AGTTCAGCAG TACAGGATCA TCTATTCTCC CACTGTTGGT 
GATCCAATTG ATGAATATAC CACAGTCCCA GGCAGAAGAA ACAATGTAAT ACTGCAGCCC 
CTGCAACCTG ACACTCCATA TAAAATTACT GTTATTGCTG TTTATGAAGA TGGAGATGGT 
GGCCATCTAA CAGGAAATGG AAGAACTGTG GGACTCCTTC CTCCTCAGAA CATACACATC 
TCTGACGAAT GGTATACAAG ATTCAGGGTG TCCTGGGATC CTTCACCTTC TCCAGTTCTT 
GGATATAAAA TAGTATATAA GCCAGTGGGT TCCAATGAGC CCATGGAAGC CTTTGTTGGA 
GAAATGACAT CATATACCTT ACACAATCTC AATCCCAGCA CCACCTACGA TGTGAATGTT 
TATGCTCAAT ATGATTCTGG ACTCAGTGTC CCCTTGACAG ATCAAGGCAC TACATTATAT 
TTAAATGTAA CAGATCTGAA AACTTACCAG ATTGGGTGGG ATACATTCTG TGTCAAATGG 
TCACCTCACC GGGCAGCCAC CTCCTACAGG CTAAAACTAA GCCCTGCAGA TGGAACAAGG 
GGACAAGAAA TTACAGTGCG TGGATCAGAA ACCAGTCACT GCTTCACTGG CCTTTCACCA 
GACACTGATT ATGGTGTCAC TGTTTTTGTG CAGACACCAA ATCTCGAGGG ACCAGGAGTC 
TCTGTTAAAG AACATACCAC TGTGAAACCA ACAGAAGCCC CTACAGAGCC ACCCACACCT 
CCTCCCCCTC CCACCATTCC ACCAGCCCGG GATGTATGCA AAGGGGCCAA GGCAGATATT 
GTGTTCTTGA CTGATGCCTC CTGGAGCATT GGGGACGATA ATTTTAACAA AGTTGTAAAA 
TTCATCTTCA ATACTGTGGG AGGCTTTGAT GAAATCAGTC CTGCTGGGAT TCAGGTTTCA 
TTTGTGCAAT ACAGCGATGA GGTCAAGTCT GAGTTCAAGC TGAACACGTA CAATGACAAG 
GCCCTAGCCC TTGGGGCCCT CCAGAATATT AGGTACAGAG GAGGAAACAC AAGAACAGGC 
AAGGCCCTCA CGTTTATCAA GGAGAAAGTC TTGACTTGGG AGAGCGGCAT GAGGAAGAAT 
GTCCCTAAGG TGTTGGTTGT GGTCACGGAC GGTCGGTCCC AGGATGAGGT CAAGAAGGCG 
GCTTTGGTCA TCCAGCAGTC AGGGTTCAGT GTCTTTGTAG TTGGTGTGGC TGATGTCGAC 
TACAATGAGC TTGCCAACAT TGCCAGCAAA CCAAGTGAAC GGCACGTGTT CATTGTGGAC 
GACTTTGAAT CTTTTGAGAA GATCGAAGAC AATCTTATTA CATTTGTTTG TGAAACTGCC 
ACTTCAAGTT GTCCTCTCAT TTATTTGGAT GGCTACACCT CACCAGGTTT TAAAATGCTT 
GAAGCATACA ACCTGACAGA AAAGAATTTT GCTTCTGTAC AAGGAGTATC TTTGGAGTCA 
GGGTCTTTCC CCAGCTACTC AGCATACAGG ATTCAGAAGA ATGCGTTTGT GAATCAGCCT 
ACAGCAGACC TACACCCAAA TGGACTCCCT CCTTCATACA CGATTATATT ATTATTCAGA 
CTTCTCCCAG AAACTCCCAG TGACCCTTTT GCAATTTGGC AAATCACAGA CAGAGACTAC 
AAACCACAAG TTGGAGTGAT TGCAGATCCT TCTAGCAAGA CGTTATCATT CTTTAACAAG 
GATACAAGAG GCGAGGTGCA AACTGTTACA TTTGACACAG AAGAAGTAAA GACATTATTT 
TATGGAAGTT TTCACAAGGT TCATATTGTA GTGACCTCAA AAAGTGTTAA GATTTACATT 
GACTGCTATG AAATTATAGA AAAAGACATC AAGGAAGCTG GAAATATAAC AACTGATGGT 
TATGAAATTC TTGGAAAACT CCTTAAAGGG GAAAGGAAAT CAGCCGCATT CCAAATCCAG 
AGTTTTGACA TTGTCTGCAG TCCAGTGTGG ACCAGTAGAG ACAGATGCTG TGATATTCCC 
TCTAGGAGAG ATGAGGGAAA ATGCCCTGCT TTTCCAAATT CTTGCACATG TACACAGGAC 
AGCGTTGGAC CTCCAGGACC TCCAGGCCCT GCAGGAGGAC CTGGTGCTAA AGGTCCCAGA 
GGTGAAAGAG GTATCAGTGG GGCAATTGGG CCCCCTGGTC CTCGTGGAGA CATAGGTCCT 
CCAGGCCCCC AGGGTCCTCC AGGCCCTCAG GGACCCAATG GACTCTCTAT TCCGGGAGAG 
CAAGGTCGCC AAGGGATGAA AGGTGATGCT GGAGAGCCAG GACTTCCAGG CCGAACAGGA 
ACCCCAGGAT TACCTGGCCC ACCAGGACCA ATGGGACCTC CAGGAGACAG AGGCTTCACT 
GGAAAAGACG GTGCAATGGG ACCCAGGGGC CCACCAGGGC CGCCGGGAAG CCCAGGCTCC 
CCAGGAGTCA CAGGACCAAG TGGGAAGCCA GGAAAACCTG GAGATCATGG CAGACCAGGT 
CCATCTGGGT TGAAAGGAGA AAAAGGTGAT AGGGGAGACA TTGCTTCCCA GAACATGATG 
CGAGCAGTTG CAAGACAAGT CTGTGAACAA TTGATAAGTG GTCAGATGAA CAGATTCAAT 
CAGATGCTGA ATCAGATTCC AAATGATTAC CAGTCCAGTC GCAACCAGCC AGGCCCGCCG 
GGTCCACCGG GACCTCCTGG TAGCGCAGGA GCCAGAGGAG AACCTGGGCC TGGGGGGCGG 
CCAGGCTTCC CGGGCACACC AGGGATGCAG GGACCCCCTG GGGAACGAGG TTTGCCAGGA 
GAGAAAGGTG AAAGGGGTAC TGGATCTTCA GGACCTCGGG GGCTGCCTGG CCCCCCAGGT 
CCACAAGGAG AATCCAGAAC AGGTCCACCA GGGTCCACAG GTTCAAGAGG TCCCCCTGGC 
CCCCCTGGCC GTCCTGGAAA CTCAGGTATC CGAGGACCCC CAGGTCCTCC TGGATACTGT 
GATTCTTCTC AGTGTGCCAG CATCCCATAC AACGGGCAAG GCTATCCAGG TTCCGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL12A1 ( XM_011535434.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011533736.1 Homo sapiens collagen type XII alpha 1 chain (COL12A1), transcript variant X1, mRNA.

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CloneID	OHu57022
Clone ID Related Accession (Same CDS sequence)	XM_011535434.1
Accession Version	XM_011535434.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(XII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGTA GGCTTCCCCC AGCGCTTGCC GCCCTGGGCG CGGCCCTGCT CCTGTCTTCC 
ATTGAGGCAG AAGTTGACCC ACCTTCAGAC TTGAATTTTA AAATTATAGA TGAAAATACT 
GTTCATATGT CATGGGCAAA ACCAGTTGAT CCAATTGTGG GTTACAGAAT AACGGTGGAC 
CCTACAACGG ATGGGCCTAC TAAAGAATTT ACCCTTTCAG CTAGTACCAC TGAAACTTTA 
TTGTCAGAAC TTGTACCTGA AACAGAGTAT GTGGTGACAA TAACTTCATA TGATGAAGTA 
GAAGAAAGTG TACCAGTTAT AGGACAACTA ACAATTCAAA CAGGTAGTTC GACAAAGCCA 
GTGGAGAAGA AACCTGGAAA AACCGAGATA CAAAAATGCT CTGTCAGTGC CTGGACTGAT 
TTGGTTTTCC TCGTGGATGG CTCTTGGAGT GTGGGAAGAA ATAATTTCAA GTACATTTTA 
GACTTCATTG CTGCTCTTGT GTCTGCTTTT GACATTGGGG AAGAGAAGAC AAGAGTTGGA 
GTTGTTCAAT ACAGCTCTGA TACCAGGACT GAATTTAACT TAAATCAGTA CTACCAAAGG 
GATGAACTTC TTGCTGCAAT AAAAAAAATT CCATATAAAG GTGGCAACAC AATGACAGGG 
GATGCCATTG ATTATTTAGT TAAAAATACT TTCACGGAAT CTGCTGGGGC AAGAGTTGGC 
TTTCCTAAAG TGGCAATTAT TATTACGGAT GGAAAATCCC AGGATGAAGT GGAAATTCCA 
GCAAGAGAGC TTCGTAATGT TGGAGTTGAA GTTTTCTCCT TGGGCATTAA AGCTGCAGAT 
GCAAAAGAAC TCAAACAAAT TGCCTCCACA CCTTCACTGA ACCATGTTTT CAATGTGGCC 
AACTTTGATG CAATTGTGGA TATTCAGAAT GAGATCATCT CCCAGGTGTG CTCAGGTGTT 
GATGAACAAC TTGGTGAATT GGTTAGTGGA GAAGAAGTTG TTGAGCCTCC TTCAAATTTG 
ATTGCCATGG AAGTCTCTTC AAAATATGTT AAGCTAAATT GGAATCCATC TCCTAGTCCA 
GTGACTGGCT ACAAAGTCAT CCTCACACCA ATGACTGCAG GAAGCCGACA GCACGCTCTG 
AGTGTGGGGC CTCAGACAAC CACGCTCAGT GTTCGCGACC TCTCAGCAGA CACAGAATAC 
CAGATCAGTG TTTCCGCCAT GAAGGGAATG ACATCCAGTG AACCCATTTC AATAATGGAG 
AAGACTCAGC CAATGAAAGT TCAAGTGGAA TGCTCACGTG GTGTGGATAT AAAAGCCGAT 
ATTGTGTTTT TGGTTGATGG CTCCTATAGC ATTGGGATTG CAAACTTTGT TAAAGTTAGA 
GCCTTTTTGG AAGTTCTTGT AAAAAGTTTT GAAATTTCAC CAAATAGGGT CCAGATTAGT 
CTTGTGCAAT ACAGCCGGGA TCCTCATACT GAGTTCACTT TGAAAAAATT CACCAAAGTT 
GAAGATATAA TTGAAGCAAT AAACACCTTC CCTTACAGAG GAGGATCTAC AAATACTGGC 
AAAGCAATGA CTTATGTCAG AGAGAAAATA TTTGTGCCTA GCAAGGGATC AAGAAGCAAT 
GTGCCAAAGG TCATGATTCT TATCACGGAT GGGAAATCAT CAGATGCTTT CAGAGATCCT 
GCGATAAAAC TGAGGAATTC AGATGTTGAA ATCTTTGCAG TTGGTGTGAA GGATGCCGTT 
CGCTCAGAAT TGGAAGCTAT TGCCTCTCCT CCTGCAGAGA CCCATGTGTT CACAGTGGAA 
GATTTTGATG CTTTTCAGAG GATATCTTTT GAACTCACAC AGTCTATCTG CCTTAGAATT 
GAGCAAGAAT TGGCAGCTAT AAAGAAGAAA GCTTACGTCC CTCCAAAGGA TCTTAGTTTT 
TCAGAAGTGA CTTCTTATGG TTTCAAAACC AACTGGTCTC CAGCTGGAGA AAATGTTTTT 
TCATATCACA TCACCTACAA GGAAGCGGCT GGGGATGATG AGGTCACTGT GGTGGAGCCA 
GCATCGAGCA CCAGTGTTGT TCTCAGCAGC CTGAAGCCAG AGACCTTGTA TTTGGTCAAT 
GTGACTGCGG AGTATGAGGA TGGCTTCAGC ATTCCCTTAG CTGGAGAGGA GACCACCGAA 
GAAGTAAAAG GAGCACCTCG AAACCTAAAG GTGACAGATG AGACTACAGA TAGTTTCAAA 
ATTACTTGGA CTCAAGCTCC AGGGAGAGTT TTAAGATATC GAATTATATA TAGACCAGTT 
GCTGGTGGAG AGAGCAGAGA AGTTACCACC CCACCCAATC AGAGGAGGAG AACACTGGAG 
AACTTGATTC CAGACACGAA ATATGAAGTA TCTGTAATTC CTGAATATTT CTCAGGACCT 
GGTACTCCAT TAACTGGAAA TGCAGCCACT GAAGAAGTTA GAGGGAATCC AAGAGACTTA 
AGAGTTTCTG ACCCTACGAC GTCTACTATG AAATTATCTT GGAGTGGGGC ACCAGGAAAA 
GTGAAACAGT ATCTCGTCAC ATATACCCCA GTGGCAGGGG GTGAAACTCA AGAGGTCACT 
GTGAGGGGAG ATACAACCAA TACGGTGCTG CAGGGATTGA AGGAAGGGAC ACAATACGCC 
TTATCTGTGA CAGCCTTGTA TGCGTCTGGG GCTGGAGACG CCCTCTTTGG TGAAGGAACA 
ACACTTGAAG AACGTGGTTC TCCTCAAGAT TTAGTTACTA AAGACATCAC TGACACATCA 
ATTGGGGCTT ATTGGACATC TGCTCCAGGA ATGGTTCGCG GTTACAGGGT CTCATGGAAA 
TCACTTTATG ATGATGTTGA CACTGGAGAG AAAAATCTGC CTGAAGATGC AATTCATACG 
ATGATAGAAA ATCTGCAGCC AGAGACCAAA TACAGAATTT CAGTATTTGC CACTTACAGC 
AGTGGAGAAG GAGAACCTTT GACTGGAGAT GCCACAACTG AATTATCTCA AGATTCCAAA 
ACCCTGAAAG TAGATGAAGA AACAGAAAAC ACAATGAGAG TTACATGGAA ACCAGCACCA 
GGGAAAGTCG TCAACTACCG TGTTGTCTAT CGCCCTCATG GGAGAGGGAA GCAAATGGTT 
GCTAAGGTGC CCCCCACAGT CACTTCGACA GTGTTAAAGC GACTTCAGCC ACAGACCACA 
TATGACATCA CAGTTCTTCC TATTTACAAG ATGGGAGAAG GAAAGCTTAG GCAAGGATCA 
GGAACAACAG CTTCTCGGTT TAAGTCTCCT AGAAACCTCA AAACATCTGA CCCAACCATG 
TCAAGCTTCC GAGTGACTTG GGAGCCTGCC CCTGGGGAAG TGAAGGGTTA TAAAGTCACA 
TTCCACCCTA CGGGGGATGA CAGAAGACTG GGGGAGTTAG TGGTTGGACC CTATGACAAC 
ACAGTTGTTT TGGAGGAACT TAGGGCTGGT ACCACCTATA AAGTAAATGT TTTTGGAATG 
TTTGATGGAG GAGAAAGCTC ACCACTTGTT GGACAAGAAA TGACAACCCT TTCCGACACA 
ACTGTTATGC CAATTTTATC TTCTGGGATG GAGTGTCTCA CCAGAGCTGA GGCAGACATT 
GTGTTGCTGG TGGATGGATC ATGGAGCATC GGCCGGGCAA ATTTTAGAAC CGTGAGGAGT 
TTCATTTCTC GTATTGTGGA AGTCTTTGAC ATTGGCCCCA AAAGAGTACA AATTGCTCTT 
GCTCAGTATA GTGGGGATCC CAGAACAGAG TGGCAGTTAA ATGCACACAG AGACAAGAAG 
AGCTTGTTGC AAGCTGTGGC AAACTTGCCG TACAAAGGAG GCAATACTCT CACAGGCATG 
GCTTTGAATT TCATTCGCCA ACAGAACTTC AGGACCCAAG CTGGCATGAG ACCTCGAGCT 
CGAAAAATTG GTGTGCTCAT TACTGATGGA AAATCACAAG ACGATGTTGA AGCACCTTCA 
AAGAAACTCA AGGATGAGGG AGTGGAGCTG TTTGCTATTG GTATTAAAAA TGCTGATGAA 
GTCGAATTAA AGATGATTGC AACTGATCCT GATGATACCC ATGCATACAA TGTGGCAGAT 
TTTGAGTCAC TCTCCAGGAT AGTGGATGAT CTCACCATTA ATTTGTGTAA CAGTGTCAAA 
GGTCCAGGTG ATTTGGAAGC ACCTTCTAAC TTAGTTATTT CTGAGCGAAC CCATCGTTCT 
TTTAGAGTGA GCTGGACACC ACCTTCTGAC AGTGTGGATC GATATAAGGT GGAATACTAT 
CCAGTTTCTG GAGGGAAACG TCAAGAATTT TATGTGAGTC GAATGGAAAC TAGCACAGTG 
CTGAAAGATC TGAAACCTGA AACTGAATAT GTTGTCAATG TGTATTCTGT GGTAGAAGAT 
GAATATAGTG AGCCTCTGAA GGGGACAGAA AAAACCTTGC CAGTGCCTGT AGTCAGCCTG 
AATATTTATG ATGTTGGCCC TACCACCATG CATGTGCAGT GGCAGCCTGT GGGAGGAGCT 
ACTGGCTACA TCTTGTCATA CAAACCTGTT AAGGACACAG AGCCAACAAG ACCCAAAGAG 
GTGCGTTTGG GGCCAACAGT GAATGACATG CAGCTGACTG ACCTTGTTCC CAACACGGAG 
TATGCAGTCA CAGTCCAGGC TGTCCTGCAC GACCTCACTA GTGAACCTGT CACTGTTCGG 
GAAGTCACCT TGCCTTTACC CAGACCTCAG GATCTGAAAC TCAGAGATGT GACTCACAGC 
ACTATGAATG TCTTTTGGGA ACCTGTGCCT GGAAAAGTGC GTAAATATAT TGTTCGATAC 
AAAACACCAG AAGAGGATGT CAAAGAGGTA GAGGTGGACA GATCAGAGAC CAGCACTTCC 
CTCAAAGACC TCTTCTCACA GACCTTGTAC ACAGTCAGCG TTTCTGCAGT ACATGACGAG 
GGGGAGTCTC CTCCAGTGAC TGCTCAAGAA ACTACCCGAC CCGTGCCAGC CCCAACAAAC 
TTAAAGATTA CTGAAGTAAC ATCAGAGGGT TTCAGAGGGA CTTGGGATCA TGGAGCTTCA 
GATGTGTCTC TCTACAGAAT AACTTGGGCA CCTTTTGGAA GCTCAGATAA GATGGAGACC 
ATCTTAAATG GAGATGAAAA CACTTTGGTG TTCGAAAACC TGAACCCCAA CACCATCTAT 
GAAGTTTCCA TTACTGCCAT CTATCCTGAT GAGTCAGAAA GTGATGACCT GATTGGCAGT 
GAGCGCACAC TGCCTATCTT AACAACACAA GCTCCCAAAA GTGGCCCACG AAACCTTCAA 
GTGTACAATG CAACATCTAA CAGCCTGACT GTTAAGTGGG ATCCTGCTAG TGGTCGTGTG 
CAGAAATATA GGATCACTTA TCAGCCTTCC ACAGGGGAAG GCAATGAGCA AACGACCACA 
ATAGGAGGAC GGCAGAACAG TGTGGTCCTG CAGAAACTGA AGCCAGACAC TCCTTACACT 
ATCACCGTAT CCTCTCTGTA TCCTGATGGT GAAGGAGGTC GGATGACGGG AAGAGGCAAG 
ACCAAACCTC TAAACACTGT AAGGAACCTG AGAGTGTATG ACCCTTCTAC CAGCACCTTG 
AATGTCCGCT GGGACCATGC AGAGGGAAAT CCTCGTCAGT ACAAGCTCTT CTATGCACCA 
GCAGCAGGTG GTCCAGAGGA ACTGGTACCA ATCCCCGGGA ATACCAATTA TGCCATTCTT 
AGGAATCTGC AGCCAGATAC CTCATACACT GTGACTGTAG TTCCCGTTTA TACTGAAGGT 
GATGGGGGAC GCACATCAGA TACTGGAAGG ACATTGATGA GAGGACTGGC AAGAAATGTC 
CAAGTATACA ATCCTACACC TAACAGCCTC GATGTTCGCT GGGACCCTGC TCCAGGACCT 
GTGCTGCAAT ATCGCGTTGT GTATTCTCCT GTGGATGGCA CAAGACCCTC AGAATCTATA 
GTAGTGCCAG GAAACACGCG CATGGTGCAT CTGGAGCGGC TGATTCCGGA CACACTCTAT 
TCCGTGAACC TTGTGGCTCT GTACTCGGAT GGAGAGGGAA ATCCCAGCCC TGCCCAGGGC 
CGAACGCTAC CACGCAGTGG ACCAAGGAAC CTGAGAGTCT TTGGTGAAAC AACCAATAGC 
CTCTCGGTAG CCTGGGATCA TGCTGATGGG CCAGTTCAGC AGTACAGGAT CATCTATTCT 
CCCACTGTTG GTGATCCAAT TGATGAATAT ACCACAGTCC CAGGCAGAAG AAACAATGTA 
ATACTGCAGC CCCTGCAACC TGACACTCCA TATAAAATTA CTGTTATTGC TGTTTATGAA 
GATGGAGATG GTGGCCATCT AACAGGAAAT GGAAGAACTG TGGGACTCCT TCCTCCTCAG 
AACATACACA TCTCTGACGA ATGGTATACA AGATTCAGGG TGTCCTGGGA TCCTTCACCT 
TCTCCAGTTC TTGGATATAA AATAGTATAT AAGCCAGTGG GTTCCAATGA GCCCATGGAA 
GCCTTTGTTG GAGAAATGAC ATCATATACC TTACACAATC TCAATCCCAG CACCACCTAC 
GATGTGAATG TTTATGCTCA ATATGATTCT GGACTCAGTG TCCCCTTGAC AGATCAAGGC 
ACTACATTAT ATTTAAATGT AACAGATCTG AAAACTTACC AGATTGGGTG GGATACATTC 
TGTGTCAAAT GGTCACCTCA CCGGGCAGCC ACCTCCTACA GGCTAAAACT AAGCCCTGCA 
GATGGAACAA GGGGACAAGA AATTACAGTG CGTGGATCAG AAACCAGTCA CTGCTTCACT 
GGCCTTTCAC CAGACACTGA TTATGGTGTC ACTGTTTTTG TGCAGACACC AAATCTCGAG 
GGACCAGGAG TCTCTGTTAA AGAACATACC ACTGTGAAAC CAACAGAAGC CCCTACAGAG 
CCACCCACAC CTCCTCCCCC TCCCACCATT CCACCAGCCC GGGATGTATG CAAAGGGGCC 
AAGGCAGATA TTGTGTTCTT GACTGATGCC TCCTGGAGCA TTGGGGACGA TAATTTTAAC 
AAAGTTGTAA AATTCATCTT CAATACTGTG GGAGGCTTTG ATGAAATCAG TCCTGCTGGG 
ATTCAGGTTT CATTTGTGCA ATACAGCGAT GAGGTCAAGT CTGAGTTCAA GCTGAACACG 
TACAATGACA AGGCCCTAGC CCTTGGGGCC CTCCAGAATA TTAGGTACAG AGGAGGAAAC 
ACAAGAACAG GCAAGGCCCT CACGTTTATC AAGGAGAAAG TCTTGACTTG GGAGAGCGGC 
ATGAGGAAGA ATGTCCCTAA GGTGTTGGTT GTGGTCACGG ACGGTCGGTC CCAGGATGAG 
GTCAAGAAGG CGGCTTTGGT CATCCAGCAG TCAGGGTTCA GTGTCTTTGT AGTTGGTGTG 
GCTGATGTCG ACTACAATGA GCTTGCCAAC ATTGCCAGCA AACCAAGTGA ACGGCACGTG 
TTCATTGTGG ACGACTTTGA ATCTTTTGAG AAGATCGAAG ACAATCTTAT TACATTTGTT 
TGTGAAACTG CCACTTCAAG TTGTCCTCTC ATTTATTTGG ATGGCTACAC CTCACCAGGT 
TTTAAAATGC TTGAAGCATA CAACCTGACA GAAAAGAATT TTGCTTCTGT ACAAGGAGTA 
TCTTTGGAGT CAGGGTCTTT CCCCAGCTAC TCAGCATACA GGATTCAGAA GAATGCGTTT 
GTGAATCAGC CTACAGCAGA CCTACACCCA AATGGACTCC CTCCTTCATA CACGATTATA 
TTATTATTCA GACTTCTCCC AGAAACTCCC AGTGACCCTT TTGCAATTTG GCAAATCACA 
GACAGAGACT ACAAACCACA AGTTGGAGTG ATTGCAGATC CTTCTAGCAA GACGTTATCA 
TTCTTTAACA AGGATACAAG AGGCGAGGTG CAAACTGTTA CATTTGACAC AGAAGAAGTA 
AAGACATTAT TTTATGGAAG TTTTCACAAG GTTCATATTG TAGTGACCTC AAAAAGTGTT 
AAGATTTACA TTGACTGCTA TGAAATTATA GAAAAAGACA TCAAGGAAGC TGGAAATATA 
ACAACTGATG GTTATGAAAT TCTTGGAAAA CTCCTTAAAG GGGAAAGGAA ATCAGCCGCA 
TTCCAAATCC AGAGTTTTGA CATTGTCTGC AGTCCAGTGT GGACCAGTAG AGACAGATGC 
TGTGATATTC CCTCTAGGAG AGATGAGGGA AAATGCCCTG CTTTTCCAAA TTCTTGCACA 
TGTACACAGG ACAGCGTTGG ACCTCCAGGA CCTCCAGGCC CTGCAGGAGG ACCTGGTGCT 
AAAGGTCCCA GAGGTGAAAG AGGTATCAGT GGGGCAATTG GGCCCCCTGG TCCTCGTGGA 
GACATAGGTC CTCCAGGCCC CCAGGGTCCT CCAGGCCCTC AGGGACCCAA TGGACTCTCT 
ATTCCGGGAG AGCAAGGTCG CCAAGGGATG AAAGGTGATG CTGGAGAGCC AGGACTTCCA 
GGCCGAACAG GAACCCCAGG ATTACCTGGC CCACCAGGAC CAATGGGACC TCCAGGAGAC 
AGAGGCTTCA CTGGAAAAGA CGGTGCAATG GGACCCAGGG GCCCACCAGG GCCGCCGGGA 
AGCCCAGGCT CCCCAGGAGT CACAGGACCA AGTGGGAAGC CAGGAAAACC TGGAGATCAT 
GGCAGACCAG GTCCATCTGG GTTGAAAGGA GAAAAAGGTG ATAGGGGAGA CATTGCTTCC 
CAGAACATGA TGCGAGCAGT TGCAAGACAA GTCTGTGAAC AATTGATAAG TGGTCAGATG 
AACAGATTCA ATCAGATGCT GAATCAGATT CCAAATGATT ACCAGTCCAG TCGCAACCAG 
CCAGGCCCGC CGGGTCCACC GGGACCTCCT GGTAGCGCAG GAGCCAGAGG AGAACCTGGG 
CCTGGGGGGC GGCCAGGCTT CCCGGGCACA CCAGGGATGC AGGGACCCCC TGGGGAACGA 
GGTTTGCCAG GAGAGAAAGG TGAAAGGGGT ACTGGATCTT CAGGACCTCG GGGGCTGCCT 
GGCCCCCCAG GTCCACAAGG AGAATCCAGA ACAGGTCCAC CAGGGTCCAC AGGTTCAAGA 
GGTCCCCCTG GCCCCCCTGG CCGTCCTGGA AACTCAGGTA TCCGAGGACC CCCAGGTCCT 
CCTGGATACT GTGATTCTTC TCAGTGTGCC AGCATCCCAT ACAACGGGCA AGGCTATCCA 
GAGCCTTACG TGCCCGAGGG CGGGGCCTAC CTGCCTGAGC GCGAGCCCTT CATCGTGCCG 
GTGGAGCCCG AGCGGACAGC GGAATACGAG GACTACGGCG CAGACGAGCC TGCAGAGGAG 
CCTCCTGAGC CCCACCGGCG CTGGCGGCGC GCCCTGCCCC ACGGACCCGG GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011533736.1
CDS	311..9667
Translation	MRSRLPPALAALGAALLLSSIEAEVDPPSDLNFKIIDENTVHMSWAKPVDPIVGYRITVDPTTDGPTKEFTLSASTTETLLSELVPETEYVVTITSYDEVEESVPVIGQLTIQTGSSTKPVEKKPGKTEIQKCSVSAWTDLVFLVDGSWSVGRNNFKYILDFIAALVSAFDIGEEKTRVGVVQYSSDTRTEFNLNQYYQRDELLAAIKKIPYKGGNTMTGDAIDYLVKNTFTESAGARVGFPKVAIIITDGKSQDEVEIPARELRNVGVEVFSLGIKAADAKELKQIASTPSLNHVFNVANFDAIVDIQNEIISQVCSGVDEQLGELVSGEEVVEPPSNLIAMEVSSKYVKLNWNPSPSPVTGYKVILTPMTAGSRQHALSVGPQTTTLSVRDLSADTEYQISVSAMKGMTSSEPISIMEKTQPMKVQVECSRGVDIKADIVFLVDGSYSIGIANFVKVRAFLEVLVKSFEISPNRVQISLVQYSRDPHTEFTLKKFTKVEDIIEAINTFPYRGGSTNTGKAMTYVREKIFVPSKGSRSNVPKVMILITDGKSSDAFRDPAIKLRNSDVEIFAVGVKDAVRSELEAIASPPAETHVFTVEDFDAFQRISFELTQSICLRIEQELAAIKKKAYVPPKDLSFSEVTSYGFKTNWSPAGENVFSYHITYKEAAGDDEVTVVEPASSTSVVLSSLKPETLYLVNVTAEYEDGFSIPLAGEETTEEVKGAPRNLKVTDETTDSFKITWTQAPGRVLRYRIIYRPVAGGESREVTTPPNQRRRTLENLIPDTKYEVSVIPEYFSGPGTPLTGNAATEEVRGNPRDLRVSDPTTSTMKLSWSGAPGKVKQYLVTYTPVAGGETQEVTVRGDTTNTVLQGLKEGTQYALSVTALYASGAGDALFGEGTTLEERGSPQDLVTKDITDTSIGAYWTSAPGMVRGYRVSWKSLYDDVDTGEKNLPEDAIHTMIENLQPETKYRISVFATYSSGEGEPLTGDATTELSQDSKTLKVDEETENTMRVTWKPAPGKVVNYRVVYRPHGRGKQMVAKVPPTVTSTVLKRLQPQTTYDITVLPIYKMGEGKLRQGSGTTASRFKSPRNLKTSDPTMSSFRVTWEPAPGEVKGYKVTFHPTGDDRRLGELVVGPYDNTVVLEELRAGTTYKVNVFGMFDGGESSPLVGQEMTTLSDTTVMPILSSGMECLTRAEADIVLLVDGSWSIGRANFRTVRSFISRIVEVFDIGPKRVQIALAQYSGDPRTEWQLNAHRDKKSLLQAVANLPYKGGNTLTGMALNFIRQQNFRTQAGMRPRARKIGVLITDGKSQDDVEAPSKKLKDEGVELFAIGIKNADEVELKMIATDPDDTHAYNVADFESLSRIVDDLTINLCNSVKGPGDLEAPSNLVISERTHRSFRVSWTPPSDSVDRYKVEYYPVSGGKRQEFYVSRMETSTVLKDLKPETEYVVNVYSVVEDEYSEPLKGTEKTLPVPVVSLNIYDVGPTTMHVQWQPVGGATGYILSYKPVKDTEPTRPKEVRLGPTVNDMQLTDLVPNTEYAVTVQAVLHDLTSEPVTVREVTLPLPRPQDLKLRDVTHSTMNVFWEPVPGKVRKYIVRYKTPEEDVKEVEVDRSETSTSLKDLFSQTLYTVSVSAVHDEGESPPVTAQETTRPVPAPTNLKITEVTSEGFRGTWDHGASDVSLYRITWAPFGSSDKMETILNGDENTLVFENLNPNTIYEVSITAIYPDESESDDLIGSERTLPILTTQAPKSGPRNLQVYNATSNSLTVKWDPASGRVQKYRITYQPSTGEGNEQTTTIGGRQNSVVLQKLKPDTPYTITVSSLYPDGEGGRMTGRGKTKPLNTVRNLRVYDPSTSTLNVRWDHAEGNPRQYKLFYAPAAGGPEELVPIPGNTNYAILRNLQPDTSYTVTVVPVYTEGDGGRTSDTGRTLMRGLARNVQVYNPTPNSLDVRWDPAPGPVLQYRVVYSPVDGTRPSESIVVPGNTRMVHLERLIPDTLYSVNLVALYSDGEGNPSPAQGRTLPRSGPRNLRVFGETTNSLSVAWDHADGPVQQYRIIYSPTVGDPIDEYTTVPGRRNNVILQPLQPDTPYKITVIAVYEDGDGGHLTGNGRTVGLLPPQNIHISDEWYTRFRVSWDPSPSPVLGYKIVYKPVGSNEPMEAFVGEMTSYTLHNLNPSTTYDVNVYAQYDSGLSVPLTDQGTTLYLNVTDLKTYQIGWDTFCVKWSPHRAATSYRLKLSPADGTRGQEITVRGSETSHCFTGLSPDTDYGVTVFVQTPNLEGPGVSVKEHTTVKPTEAPTEPPTPPPPPTIPPARDVCKGAKADIVFLTDASWSIGDDNFNKVVKFIFNTVGGFDEISPAGIQVSFVQYSDEVKSEFKLNTYNDKALALGALQNIRYRGGNTRTGKALTFIKEKVLTWESGMRKNVPKVLVVVTDGRSQDEVKKAALVIQQSGFSVFVVGVADVDYNELANIASKPSERHVFIVDDFESFEKIEDNLITFVCETATSSCPLIYLDGYTSPGFKMLEAYNLTEKNFASVQGVSLESGSFPSYSAYRIQKNAFVNQPTADLHPNGLPPSYTIILLFRLLPETPSDPFAIWQITDRDYKPQVGVIADPSSKTLSFFNKDTRGEVQTVTFDTEEVKTLFYGSFHKVHIVVTSKSVKIYIDCYEIIEKDIKEAGNITTDGYEILGKLLKGERKSAAFQIQSFDIVCSPVWTSRDRCCDIPSRRDEGKCPAFPNSCTCTQDSVGPPGPPGPAGGPGAKGPRGERGISGAIGPPGPRGDIGPPGPQGPPGPQGPNGLSIPGEQGRQGMKGDAGEPGLPGRTGTPGLPGPPGPMGPPGDRGFTGKDGAMGPRGPPGPPGSPGSPGVTGPSGKPGKPGDHGRPGPSGLKGEKGDRGDIASQNMMRAVARQVCEQLISGQMNRFNQMLNQIPNDYQSSRNQPGPPGPPGPPGSAGARGEPGPGGRPGFPGTPGMQGPPGERGLPGEKGERGTGSSGPRGLPGPPGPQGESRTGPPGSTGSRGPPGPPGRPGNSGIRGPPGPPGYCDSSQCASIPYNGQGYPEPYVPEGGAYLPEREPFIVPVEPERTAEYEDYGADEPAEEPPEPHRRWRRALPHGPGQ

Target ORF information:

RefSeq Version	XM_011535434.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XII alpha 1 chain (COL12A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535434.1

ORF Insert Sequence:

ATGCGGAGTA GGCTTCCCCC AGCGCTTGCC GCCCTGGGCG CGGCCCTGCT CCTGTCTTCC 
ATTGAGGCAG AAGTTGACCC ACCTTCAGAC TTGAATTTTA AAATTATAGA TGAAAATACT 
GTTCATATGT CATGGGCAAA ACCAGTTGAT CCAATTGTGG GTTACAGAAT AACGGTGGAC 
CCTACAACGG ATGGGCCTAC TAAAGAATTT ACCCTTTCAG CTAGTACCAC TGAAACTTTA 
TTGTCAGAAC TTGTACCTGA AACAGAGTAT GTGGTGACAA TAACTTCATA TGATGAAGTA 
GAAGAAAGTG TACCAGTTAT AGGACAACTA ACAATTCAAA CAGGTAGTTC GACAAAGCCA 
GTGGAGAAGA AACCTGGAAA AACCGAGATA CAAAAATGCT CTGTCAGTGC CTGGACTGAT 
TTGGTTTTCC TCGTGGATGG CTCTTGGAGT GTGGGAAGAA ATAATTTCAA GTACATTTTA 
GACTTCATTG CTGCTCTTGT GTCTGCTTTT GACATTGGGG AAGAGAAGAC AAGAGTTGGA 
GTTGTTCAAT ACAGCTCTGA TACCAGGACT GAATTTAACT TAAATCAGTA CTACCAAAGG 
GATGAACTTC TTGCTGCAAT AAAAAAAATT CCATATAAAG GTGGCAACAC AATGACAGGG 
GATGCCATTG ATTATTTAGT TAAAAATACT TTCACGGAAT CTGCTGGGGC AAGAGTTGGC 
TTTCCTAAAG TGGCAATTAT TATTACGGAT GGAAAATCCC AGGATGAAGT GGAAATTCCA 
GCAAGAGAGC TTCGTAATGT TGGAGTTGAA GTTTTCTCCT TGGGCATTAA AGCTGCAGAT 
GCAAAAGAAC TCAAACAAAT TGCCTCCACA CCTTCACTGA ACCATGTTTT CAATGTGGCC 
AACTTTGATG CAATTGTGGA TATTCAGAAT GAGATCATCT CCCAGGTGTG CTCAGGTGTT 
GATGAACAAC TTGGTGAATT GGTTAGTGGA GAAGAAGTTG TTGAGCCTCC TTCAAATTTG 
ATTGCCATGG AAGTCTCTTC AAAATATGTT AAGCTAAATT GGAATCCATC TCCTAGTCCA 
GTGACTGGCT ACAAAGTCAT CCTCACACCA ATGACTGCAG GAAGCCGACA GCACGCTCTG 
AGTGTGGGGC CTCAGACAAC CACGCTCAGT GTTCGCGACC TCTCAGCAGA CACAGAATAC 
CAGATCAGTG TTTCCGCCAT GAAGGGAATG ACATCCAGTG AACCCATTTC AATAATGGAG 
AAGACTCAGC CAATGAAAGT TCAAGTGGAA TGCTCACGTG GTGTGGATAT AAAAGCCGAT 
ATTGTGTTTT TGGTTGATGG CTCCTATAGC ATTGGGATTG CAAACTTTGT TAAAGTTAGA 
GCCTTTTTGG AAGTTCTTGT AAAAAGTTTT GAAATTTCAC CAAATAGGGT CCAGATTAGT 
CTTGTGCAAT ACAGCCGGGA TCCTCATACT GAGTTCACTT TGAAAAAATT CACCAAAGTT 
GAAGATATAA TTGAAGCAAT AAACACCTTC CCTTACAGAG GAGGATCTAC AAATACTGGC 
AAAGCAATGA CTTATGTCAG AGAGAAAATA TTTGTGCCTA GCAAGGGATC AAGAAGCAAT 
GTGCCAAAGG TCATGATTCT TATCACGGAT GGGAAATCAT CAGATGCTTT CAGAGATCCT 
GCGATAAAAC TGAGGAATTC AGATGTTGAA ATCTTTGCAG TTGGTGTGAA GGATGCCGTT 
CGCTCAGAAT TGGAAGCTAT TGCCTCTCCT CCTGCAGAGA CCCATGTGTT CACAGTGGAA 
GATTTTGATG CTTTTCAGAG GATATCTTTT GAACTCACAC AGTCTATCTG CCTTAGAATT 
GAGCAAGAAT TGGCAGCTAT AAAGAAGAAA GCTTACGTCC CTCCAAAGGA TCTTAGTTTT 
TCAGAAGTGA CTTCTTATGG TTTCAAAACC AACTGGTCTC CAGCTGGAGA AAATGTTTTT 
TCATATCACA TCACCTACAA GGAAGCGGCT GGGGATGATG AGGTCACTGT GGTGGAGCCA 
GCATCGAGCA CCAGTGTTGT TCTCAGCAGC CTGAAGCCAG AGACCTTGTA TTTGGTCAAT 
GTGACTGCGG AGTATGAGGA TGGCTTCAGC ATTCCCTTAG CTGGAGAGGA GACCACCGAA 
GAAGTAAAAG GAGCACCTCG AAACCTAAAG GTGACAGATG AGACTACAGA TAGTTTCAAA 
ATTACTTGGA CTCAAGCTCC AGGGAGAGTT TTAAGATATC GAATTATATA TAGACCAGTT 
GCTGGTGGAG AGAGCAGAGA AGTTACCACC CCACCCAATC AGAGGAGGAG AACACTGGAG 
AACTTGATTC CAGACACGAA ATATGAAGTA TCTGTAATTC CTGAATATTT CTCAGGACCT 
GGTACTCCAT TAACTGGAAA TGCAGCCACT GAAGAAGTTA GAGGGAATCC AAGAGACTTA 
AGAGTTTCTG ACCCTACGAC GTCTACTATG AAATTATCTT GGAGTGGGGC ACCAGGAAAA 
GTGAAACAGT ATCTCGTCAC ATATACCCCA GTGGCAGGGG GTGAAACTCA AGAGGTCACT 
GTGAGGGGAG ATACAACCAA TACGGTGCTG CAGGGATTGA AGGAAGGGAC ACAATACGCC 
TTATCTGTGA CAGCCTTGTA TGCGTCTGGG GCTGGAGACG CCCTCTTTGG TGAAGGAACA 
ACACTTGAAG AACGTGGTTC TCCTCAAGAT TTAGTTACTA AAGACATCAC TGACACATCA 
ATTGGGGCTT ATTGGACATC TGCTCCAGGA ATGGTTCGCG GTTACAGGGT CTCATGGAAA 
TCACTTTATG ATGATGTTGA CACTGGAGAG AAAAATCTGC CTGAAGATGC AATTCATACG 
ATGATAGAAA ATCTGCAGCC AGAGACCAAA TACAGAATTT CAGTATTTGC CACTTACAGC 
AGTGGAGAAG GAGAACCTTT GACTGGAGAT GCCACAACTG AATTATCTCA AGATTCCAAA 
ACCCTGAAAG TAGATGAAGA AACAGAAAAC ACAATGAGAG TTACATGGAA ACCAGCACCA 
GGGAAAGTCG TCAACTACCG TGTTGTCTAT CGCCCTCATG GGAGAGGGAA GCAAATGGTT 
GCTAAGGTGC CCCCCACAGT CACTTCGACA GTGTTAAAGC GACTTCAGCC ACAGACCACA 
TATGACATCA CAGTTCTTCC TATTTACAAG ATGGGAGAAG GAAAGCTTAG GCAAGGATCA 
GGAACAACAG CTTCTCGGTT TAAGTCTCCT AGAAACCTCA AAACATCTGA CCCAACCATG 
TCAAGCTTCC GAGTGACTTG GGAGCCTGCC CCTGGGGAAG TGAAGGGTTA TAAAGTCACA 
TTCCACCCTA CGGGGGATGA CAGAAGACTG GGGGAGTTAG TGGTTGGACC CTATGACAAC 
ACAGTTGTTT TGGAGGAACT TAGGGCTGGT ACCACCTATA AAGTAAATGT TTTTGGAATG 
TTTGATGGAG GAGAAAGCTC ACCACTTGTT GGACAAGAAA TGACAACCCT TTCCGACACA 
ACTGTTATGC CAATTTTATC TTCTGGGATG GAGTGTCTCA CCAGAGCTGA GGCAGACATT 
GTGTTGCTGG TGGATGGATC ATGGAGCATC GGCCGGGCAA ATTTTAGAAC CGTGAGGAGT 
TTCATTTCTC GTATTGTGGA AGTCTTTGAC ATTGGCCCCA AAAGAGTACA AATTGCTCTT 
GCTCAGTATA GTGGGGATCC CAGAACAGAG TGGCAGTTAA ATGCACACAG AGACAAGAAG 
AGCTTGTTGC AAGCTGTGGC AAACTTGCCG TACAAAGGAG GCAATACTCT CACAGGCATG 
GCTTTGAATT TCATTCGCCA ACAGAACTTC AGGACCCAAG CTGGCATGAG ACCTCGAGCT 
CGAAAAATTG GTGTGCTCAT TACTGATGGA AAATCACAAG ACGATGTTGA AGCACCTTCA 
AAGAAACTCA AGGATGAGGG AGTGGAGCTG TTTGCTATTG GTATTAAAAA TGCTGATGAA 
GTCGAATTAA AGATGATTGC AACTGATCCT GATGATACCC ATGCATACAA TGTGGCAGAT 
TTTGAGTCAC TCTCCAGGAT AGTGGATGAT CTCACCATTA ATTTGTGTAA CAGTGTCAAA 
GGTCCAGGTG ATTTGGAAGC ACCTTCTAAC TTAGTTATTT CTGAGCGAAC CCATCGTTCT 
TTTAGAGTGA GCTGGACACC ACCTTCTGAC AGTGTGGATC GATATAAGGT GGAATACTAT 
CCAGTTTCTG GAGGGAAACG TCAAGAATTT TATGTGAGTC GAATGGAAAC TAGCACAGTG 
CTGAAAGATC TGAAACCTGA AACTGAATAT GTTGTCAATG TGTATTCTGT GGTAGAAGAT 
GAATATAGTG AGCCTCTGAA GGGGACAGAA AAAACCTTGC CAGTGCCTGT AGTCAGCCTG 
AATATTTATG ATGTTGGCCC TACCACCATG CATGTGCAGT GGCAGCCTGT GGGAGGAGCT 
ACTGGCTACA TCTTGTCATA CAAACCTGTT AAGGACACAG AGCCAACAAG ACCCAAAGAG 
GTGCGTTTGG GGCCAACAGT GAATGACATG CAGCTGACTG ACCTTGTTCC CAACACGGAG 
TATGCAGTCA CAGTCCAGGC TGTCCTGCAC GACCTCACTA GTGAACCTGT CACTGTTCGG 
GAAGTCACCT TGCCTTTACC CAGACCTCAG GATCTGAAAC TCAGAGATGT GACTCACAGC 
ACTATGAATG TCTTTTGGGA ACCTGTGCCT GGAAAAGTGC GTAAATATAT TGTTCGATAC 
AAAACACCAG AAGAGGATGT CAAAGAGGTA GAGGTGGACA GATCAGAGAC CAGCACTTCC 
CTCAAAGACC TCTTCTCACA GACCTTGTAC ACAGTCAGCG TTTCTGCAGT ACATGACGAG 
GGGGAGTCTC CTCCAGTGAC TGCTCAAGAA ACTACCCGAC CCGTGCCAGC CCCAACAAAC 
TTAAAGATTA CTGAAGTAAC ATCAGAGGGT TTCAGAGGGA CTTGGGATCA TGGAGCTTCA 
GATGTGTCTC TCTACAGAAT AACTTGGGCA CCTTTTGGAA GCTCAGATAA GATGGAGACC 
ATCTTAAATG GAGATGAAAA CACTTTGGTG TTCGAAAACC TGAACCCCAA CACCATCTAT 
GAAGTTTCCA TTACTGCCAT CTATCCTGAT GAGTCAGAAA GTGATGACCT GATTGGCAGT 
GAGCGCACAC TGCCTATCTT AACAACACAA GCTCCCAAAA GTGGCCCACG AAACCTTCAA 
GTGTACAATG CAACATCTAA CAGCCTGACT GTTAAGTGGG ATCCTGCTAG TGGTCGTGTG 
CAGAAATATA GGATCACTTA TCAGCCTTCC ACAGGGGAAG GCAATGAGCA AACGACCACA 
ATAGGAGGAC GGCAGAACAG TGTGGTCCTG CAGAAACTGA AGCCAGACAC TCCTTACACT 
ATCACCGTAT CCTCTCTGTA TCCTGATGGT GAAGGAGGTC GGATGACGGG AAGAGGCAAG 
ACCAAACCTC TAAACACTGT AAGGAACCTG AGAGTGTATG ACCCTTCTAC CAGCACCTTG 
AATGTCCGCT GGGACCATGC AGAGGGAAAT CCTCGTCAGT ACAAGCTCTT CTATGCACCA 
GCAGCAGGTG GTCCAGAGGA ACTGGTACCA ATCCCCGGGA ATACCAATTA TGCCATTCTT 
AGGAATCTGC AGCCAGATAC CTCATACACT GTGACTGTAG TTCCCGTTTA TACTGAAGGT 
GATGGGGGAC GCACATCAGA TACTGGAAGG ACATTGATGA GAGGACTGGC AAGAAATGTC 
CAAGTATACA ATCCTACACC TAACAGCCTC GATGTTCGCT GGGACCCTGC TCCAGGACCT 
GTGCTGCAAT ATCGCGTTGT GTATTCTCCT GTGGATGGCA CAAGACCCTC AGAATCTATA 
GTAGTGCCAG GAAACACGCG CATGGTGCAT CTGGAGCGGC TGATTCCGGA CACACTCTAT 
TCCGTGAACC TTGTGGCTCT GTACTCGGAT GGAGAGGGAA ATCCCAGCCC TGCCCAGGGC 
CGAACGCTAC CACGCAGTGG ACCAAGGAAC CTGAGAGTCT TTGGTGAAAC AACCAATAGC 
CTCTCGGTAG CCTGGGATCA TGCTGATGGG CCAGTTCAGC AGTACAGGAT CATCTATTCT 
CCCACTGTTG GTGATCCAAT TGATGAATAT ACCACAGTCC CAGGCAGAAG AAACAATGTA 
ATACTGCAGC CCCTGCAACC TGACACTCCA TATAAAATTA CTGTTATTGC TGTTTATGAA 
GATGGAGATG GTGGCCATCT AACAGGAAAT GGAAGAACTG TGGGACTCCT TCCTCCTCAG 
AACATACACA TCTCTGACGA ATGGTATACA AGATTCAGGG TGTCCTGGGA TCCTTCACCT 
TCTCCAGTTC TTGGATATAA AATAGTATAT AAGCCAGTGG GTTCCAATGA GCCCATGGAA 
GCCTTTGTTG GAGAAATGAC ATCATATACC TTACACAATC TCAATCCCAG CACCACCTAC 
GATGTGAATG TTTATGCTCA ATATGATTCT GGACTCAGTG TCCCCTTGAC AGATCAAGGC 
ACTACATTAT ATTTAAATGT AACAGATCTG AAAACTTACC AGATTGGGTG GGATACATTC 
TGTGTCAAAT GGTCACCTCA CCGGGCAGCC ACCTCCTACA GGCTAAAACT AAGCCCTGCA 
GATGGAACAA GGGGACAAGA AATTACAGTG CGTGGATCAG AAACCAGTCA CTGCTTCACT 
GGCCTTTCAC CAGACACTGA TTATGGTGTC ACTGTTTTTG TGCAGACACC AAATCTCGAG 
GGACCAGGAG TCTCTGTTAA AGAACATACC ACTGTGAAAC CAACAGAAGC CCCTACAGAG 
CCACCCACAC CTCCTCCCCC TCCCACCATT CCACCAGCCC GGGATGTATG CAAAGGGGCC 
AAGGCAGATA TTGTGTTCTT GACTGATGCC TCCTGGAGCA TTGGGGACGA TAATTTTAAC 
AAAGTTGTAA AATTCATCTT CAATACTGTG GGAGGCTTTG ATGAAATCAG TCCTGCTGGG 
ATTCAGGTTT CATTTGTGCA ATACAGCGAT GAGGTCAAGT CTGAGTTCAA GCTGAACACG 
TACAATGACA AGGCCCTAGC CCTTGGGGCC CTCCAGAATA TTAGGTACAG AGGAGGAAAC 
ACAAGAACAG GCAAGGCCCT CACGTTTATC AAGGAGAAAG TCTTGACTTG GGAGAGCGGC 
ATGAGGAAGA ATGTCCCTAA GGTGTTGGTT GTGGTCACGG ACGGTCGGTC CCAGGATGAG 
GTCAAGAAGG CGGCTTTGGT CATCCAGCAG TCAGGGTTCA GTGTCTTTGT AGTTGGTGTG 
GCTGATGTCG ACTACAATGA GCTTGCCAAC ATTGCCAGCA AACCAAGTGA ACGGCACGTG 
TTCATTGTGG ACGACTTTGA ATCTTTTGAG AAGATCGAAG ACAATCTTAT TACATTTGTT 
TGTGAAACTG CCACTTCAAG TTGTCCTCTC ATTTATTTGG ATGGCTACAC CTCACCAGGT 
TTTAAAATGC TTGAAGCATA CAACCTGACA GAAAAGAATT TTGCTTCTGT ACAAGGAGTA 
TCTTTGGAGT CAGGGTCTTT CCCCAGCTAC TCAGCATACA GGATTCAGAA GAATGCGTTT 
GTGAATCAGC CTACAGCAGA CCTACACCCA AATGGACTCC CTCCTTCATA CACGATTATA 
TTATTATTCA GACTTCTCCC AGAAACTCCC AGTGACCCTT TTGCAATTTG GCAAATCACA 
GACAGAGACT ACAAACCACA AGTTGGAGTG ATTGCAGATC CTTCTAGCAA GACGTTATCA 
TTCTTTAACA AGGATACAAG AGGCGAGGTG CAAACTGTTA CATTTGACAC AGAAGAAGTA 
AAGACATTAT TTTATGGAAG TTTTCACAAG GTTCATATTG TAGTGACCTC AAAAAGTGTT 
AAGATTTACA TTGACTGCTA TGAAATTATA GAAAAAGACA TCAAGGAAGC TGGAAATATA 
ACAACTGATG GTTATGAAAT TCTTGGAAAA CTCCTTAAAG GGGAAAGGAA ATCAGCCGCA 
TTCCAAATCC AGAGTTTTGA CATTGTCTGC AGTCCAGTGT GGACCAGTAG AGACAGATGC 
TGTGATATTC CCTCTAGGAG AGATGAGGGA AAATGCCCTG CTTTTCCAAA TTCTTGCACA 
TGTACACAGG ACAGCGTTGG ACCTCCAGGA CCTCCAGGCC CTGCAGGAGG ACCTGGTGCT 
AAAGGTCCCA GAGGTGAAAG AGGTATCAGT GGGGCAATTG GGCCCCCTGG TCCTCGTGGA 
GACATAGGTC CTCCAGGCCC CCAGGGTCCT CCAGGCCCTC AGGGACCCAA TGGACTCTCT 
ATTCCGGGAG AGCAAGGTCG CCAAGGGATG AAAGGTGATG CTGGAGAGCC AGGACTTCCA 
GGCCGAACAG GAACCCCAGG ATTACCTGGC CCACCAGGAC CAATGGGACC TCCAGGAGAC 
AGAGGCTTCA CTGGAAAAGA CGGTGCAATG GGACCCAGGG GCCCACCAGG GCCGCCGGGA 
AGCCCAGGCT CCCCAGGAGT CACAGGACCA AGTGGGAAGC CAGGAAAACC TGGAGATCAT 
GGCAGACCAG GTCCATCTGG GTTGAAAGGA GAAAAAGGTG ATAGGGGAGA CATTGCTTCC 
CAGAACATGA TGCGAGCAGT TGCAAGACAA GTCTGTGAAC AATTGATAAG TGGTCAGATG 
AACAGATTCA ATCAGATGCT GAATCAGATT CCAAATGATT ACCAGTCCAG TCGCAACCAG 
CCAGGCCCGC CGGGTCCACC GGGACCTCCT GGTAGCGCAG GAGCCAGAGG AGAACCTGGG 
CCTGGGGGGC GGCCAGGCTT CCCGGGCACA CCAGGGATGC AGGGACCCCC TGGGGAACGA 
GGTTTGCCAG GAGAGAAAGG TGAAAGGGGT ACTGGATCTT CAGGACCTCG GGGGCTGCCT 
GGCCCCCCAG GTCCACAAGG AGAATCCAGA ACAGGTCCAC CAGGGTCCAC AGGTTCAAGA 
GGTCCCCCTG GCCCCCCTGG CCGTCCTGGA AACTCAGGTA TCCGAGGACC CCCAGGTCCT 
CCTGGATACT GTGATTCTTC TCAGTGTGCC AGCATCCCAT ACAACGGGCA AGGCTATCCA 
GAGCCTTACG TGCCCGAGGG CGGGGCCTAC CTGCCTGAGC GCGAGCCCTT CATCGTGCCG 
GTGGAGCCCG AGCGGACAGC GGAATACGAG GACTACGGCG CAGACGAGCC TGCAGAGGAG 
CCTCCTGAGC CCCACCGGCG CTGGCGGCGC GCCCTGCCCC ACGGACCCGG GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL12A1 ( XM_011535435.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011533737.1 Homo sapiens collagen type XII alpha 1 chain (COL12A1), transcript variant X3, mRNA.

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CloneID	OHu57023
Clone ID Related Accession (Same CDS sequence)	XM_011535435.1
Accession Version	XM_011535435.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(XII) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGTA GGCTTCCCCC AGCGCTTGCC GCCCTGGGCG CGGCCCTGCT CCTGTCTTCC 
ATTGAGGCAG AAGTTGACCC ACCTTCAGAC TTGAATTTTA AAATTATAGA TGAAAATACT 
GTTCATATGT CATGGGCAAA ACCAGTTGAT CCAATTGTGG GTTACAGAAT AACGGTGGAC 
CCTACAACGG ATGGGCCTAC TAAAGAATTT ACCCTTTCAG CTAGTACCAC TGAAACTTTA 
TTGTCAGAAC TTGTACCTGA AACAGAGTAT GTGGTGACAA TAACTTCATA TGATGAAGTA 
GAAGAAAGTG TACCAGTTAT AGGACAACTA ACAATTCAAA CAGGTAGTTC GACAAAGCCA 
GTGGAGAAGA AACCTGGAAA AACCGAGATA CAAAAATGCT CTGTCAGTGC CTGGACTGAT 
TTGGTTTTCC TCGTGGATGG CTCTTGGAGT GTGGGAAGAA ATAATTTCAA GTACATTTTA 
GACTTCATTG CTGCTCTTGT GTCTGCTTTT GACATTGGGG AAGAGAAGAC AAGAGTTGGA 
GTTGTTCAAT ACAGCTCTGA TACCAGGACT GAATTTAACT TAAATCAGTA CTACCAAAGG 
GATGAACTTC TTGCTGCAAT AAAAAAAATT CCATATAAAG GTGGCAACAC AATGACAGGG 
GATGCCATTG ATTATTTAGT TAAAAATACT TTCACGGAAT CTGCTGGGGC AAGAGTTGGC 
TTTCCTAAAG TGGCAATTAT TATTACGGAT GGAAAATCCC AGGATGAAGT GGAAATTCCA 
GCAAGAGAGC TTCGTAATGT TGGAGTTGAA GTTTTCTCCT TGGGCATTAA AGCTGCAGAT 
GCAAAAGAAC TCAAACAAAT TGCCTCCACA CCTTCACTGA ACCATGTTTT CAATGTGGCC 
AACTTTGATG CAATTGTGGA TATTCAGAAT GAGATCATCT CCCAGGTGTG CTCAGGTGTT 
GATGAACAAC TTGGTGAATT GGTTAGTGGA GAAGAAGTTG TTGAGCCTCC TTCAAATTTG 
ATTGCCATGG AAGTCTCTTC AAAATATGTT AAGCTAAATT GGAATCCATC TCCTAGTCCA 
GTGACTGGCT ACAAAGTCAT CCTCACACCA ATGACTGCAG GAAGCCGACA GCACGCTCTG 
AGTGTGGGGC CTCAGACAAC CACGCTCAGT GTTCGCGACC TCTCAGCAGA CACAGAATAC 
CAGATCAGTG TTTCCGCCAT GAAGGGAATG ACATCCAGTG AACCCATTTC AATAATGGAG 
AAGACTCAGC CAATGAAAGT TCAAGTGGAA TGCTCACGTG GTGTGGATAT AAAAGCCGAT 
ATTGTGTTTT TGGTTGATGG CTCCTATAGC ATTGGGATTG CAAACTTTGT TAAAGTTAGA 
GCCTTTTTGG AAGTTCTTGT AAAAAGTTTT GAAATTTCAC CAAATAGGGT CCAGATTAGT 
CTTGTGCAAT ACAGCCGGGA TCCTCATACT GAGTTCACTT TGAAAAAATT CACCAAAGTT 
GAAGATATAA TTGAAGCAAT AAACACCTTC CCTTACAGAG GAGGATCTAC AAATACTGGC 
AAAGCAATGA CTTATGTCAG AGAGAAAATA TTTGTGCCTA GCAAGGGATC AAGAAGCAAT 
GTGCCAAAGG TCATGATTCT TATCACGGAT GGGAAATCAT CAGATGCTTT CAGAGATCCT 
GCGATAAAAC TGAGGAATTC AGATGTTGAA ATCTTTGCAG TTGGTGTGAA GGATGCCGTT 
CGCTCAGAAT TGGAAGCTAT TGCCTCTCCT CCTGCAGAGA CCCATGTGTT CACAGTGGAA 
GATTTTGATG CTTTTCAGAG GATATCTTTT GAACTCACAC AGTCTATCTG CCTTAGAATT 
GAGCAAGAAT TGGCAGCTAT AAAGAAGAAA GCTTACGTCC CTCCAAAGGA TCTTAGTTTT 
TCAGAAGTGA CTTCTTATGG TTTCAAAACC AACTGGTCTC CAGCTGGAGA AAATGTTTTT 
TCATATCACA TCACCTACAA GGAAGCGGCT GGGGATGATG AGGTCACTGT GGTGGAGCCA 
GCATCGAGCA CCAGTGTTGT TCTCAGCAGC CTGAAGCCAG AGACCTTGTA TTTGGTCAAT 
GTGACTGCGG AGTATGAGGA TGGCTTCAGC ATTCCCTTAG CTGGAGAGGA GACCACCGAA 
GAAGTAAAAG GAGCACCTCG AAACCTAAAG GTGACAGATG AGACTACAGA TAGTTTCAAA 
ATTACTTGGA CTCAAGCTCC AGGGAGAGTT TTAAGATATC GAATTATATA TAGACCAGTT 
GCTGGTGGAG AGAGCAGAGA AGTTACCACC CCACCCAATC AGAGGAGGAG AACACTGGAG 
AACTTGATTC CAGACACGAA ATATGAAGTA TCTGTAATTC CTGAATATTT CTCAGGACCT 
GGTACTCCAT TAACTGGAAA TGCAGCCACT GAAGAAGAAC GTGGTTCTCC TCAAGATTTA 
GTTACTAAAG ACATCACTGA CACATCAATT GGGGCTTATT GGACATCTGC TCCAGGAATG 
GTTCGCGGTT ACAGGGTCTC ATGGAAATCA CTTTATGATG ATGTTGACAC TGGAGAGAAA 
AATCTGCCTG AAGATGCAAT TCATACGATG ATAGAAAATC TGCAGCCAGA GACCAAATAC 
AGAATTTCAG TATTTGCCAC TTACAGCAGT GGAGAAGGAG AACCTTTGAC TGGAGATGCC 
ACAACTGAAT TATCTCAAGA TTCCAAAACC CTGAAAGTAG ATGAAGAAAC AGAAAACACA 
ATGAGAGTTA CATGGAAACC AGCACCAGGG AAAGTCGTCA ACTACCGTGT TGTCTATCGC 
CCTCATGGGA GAGGGAAGCA AATGGTTGCT AAGGTGCCCC CCACAGTCAC TTCGACAGTG 
TTAAAGCGAC TTCAGCCACA GACCACATAT GACATCACAG TTCTTCCTAT TTACAAGATG 
GGAGAAGGAA AGCTTAGGCA AGGATCAGGA ACAACAGCTT CTCGGTTTAA GTCTCCTAGA 
AACCTCAAAA CATCTGACCC AACCATGTCA AGCTTCCGAG TGACTTGGGA GCCTGCCCCT 
GGGGAAGTGA AGGGTTATAA AGTCACATTC CACCCTACGG GGGATGACAG AAGACTGGGG 
GAGTTAGTGG TTGGACCCTA TGACAACACA GTTGTTTTGG AGGAACTTAG GGCTGGTACC 
ACCTATAAAG TAAATGTTTT TGGAATGTTT GATGGAGGAG AAAGCTCACC ACTTGTTGGA 
CAAGAAATGA CAACCCTTTC CGACACAACT GTTATGCCAA TTTTATCTTC TGGGATGGAG 
TGTCTCACCA GAGCTGAGGC AGACATTGTG TTGCTGGTGG ATGGATCATG GAGCATCGGC 
CGGGCAAATT TTAGAACCGT GAGGAGTTTC ATTTCTCGTA TTGTGGAAGT CTTTGACATT 
GGCCCCAAAA GAGTACAAAT TGCTCTTGCT CAGTATAGTG GGGATCCCAG AACAGAGTGG 
CAGTTAAATG CACACAGAGA CAAGAAGAGC TTGTTGCAAG CTGTGGCAAA CTTGCCGTAC 
AAAGGAGGCA ATACTCTCAC AGGCATGGCT TTGAATTTCA TTCGCCAACA GAACTTCAGG 
ACCCAAGCTG GCATGAGACC TCGAGCTCGA AAAATTGGTG TGCTCATTAC TGATGGAAAA 
TCACAAGACG ATGTTGAAGC ACCTTCAAAG AAACTCAAGG ATGAGGGAGT GGAGCTGTTT 
GCTATTGGTA TTAAAAATGC TGATGAAGTC GAATTAAAGA TGATTGCAAC TGATCCTGAT 
GATACCCATG CATACAATGT GGCAGATTTT GAGTCACTCT CCAGGATAGT GGATGATCTC 
ACCATTAATT TGTGTAACAG TGTCAAAGGT CCAGGTGATT TGGAAGCACC TTCTAACTTA 
GTTATTTCTG AGCGAACCCA TCGTTCTTTT AGAGTGAGCT GGACACCACC TTCTGACAGT 
GTGGATCGAT ATAAGGTGGA ATACTATCCA GTTTCTGGAG GGAAACGTCA AGAATTTTAT 
GTGAGTCGAA TGGAAACTAG CACAGTGCTG AAAGATCTGA AACCTGAAAC TGAATATGTT 
GTCAATGTGT ATTCTGTGGT AGAAGATGAA TATAGTGAGC CTCTGAAGGG GACAGAAAAA 
ACCTTGCCAG TGCCTGTAGT CAGCCTGAAT ATTTATGATG TTGGCCCTAC CACCATGCAT 
GTGCAGTGGC AGCCTGTGGG AGGAGCTACT GGCTACATCT TGTCATACAA ACCTGTTAAG 
GACACAGAGC CAACAAGACC CAAAGAGGTG CGTTTGGGGC CAACAGTGAA TGACATGCAG 
CTGACTGACC TTGTTCCCAA CACGGAGTAT GCAGTCACAG TCCAGGCTGT CCTGCACGAC 
CTCACTAGTG AACCTGTCAC TGTTCGGGAA GTCACCTTGC CTTTACCCAG ACCTCAGGAT 
CTGAAACTCA GAGATGTGAC TCACAGCACT ATGAATGTCT TTTGGGAACC TGTGCCTGGA 
AAAGTGCGTA AATATATTGT TCGATACAAA ACACCAGAAG AGGATGTCAA AGAGGTAGAG 
GTGGACAGAT CAGAGACCAG CACTTCCCTC AAAGACCTCT TCTCACAGAC CTTGTACACA 
GTCAGCGTTT CTGCAGTACA TGACGAGGGG GAGTCTCCTC CAGTGACTGC TCAAGAAACT 
ACCCGACCCG TGCCAGCCCC AACAAACTTA AAGATTACTG AAGTAACATC AGAGGGTTTC 
AGAGGGACTT GGGATCATGG AGCTTCAGAT GTGTCTCTCT ACAGAATAAC TTGGGCACCT 
TTTGGAAGCT CAGATAAGAT GGAGACCATC TTAAATGGAG ATGAAAACAC TTTGGTGTTC 
GAAAACCTGA ACCCCAACAC CATCTATGAA GTTTCCATTA CTGCCATCTA TCCTGATGAG 
TCAGAAAGTG ATGACCTGAT TGGCAGTGAG CGCACACTGC CTATCTTAAC AACACAAGCT 
CCCAAAAGTG GCCCACGAAA CCTTCAAGTG TACAATGCAA CATCTAACAG CCTGACTGTT 
AAGTGGGATC CTGCTAGTGG TCGTGTGCAG AAATATAGGA TCACTTATCA GCCTTCCACA 
GGGGAAGGCA ATGAGCAAAC GACCACAATA GGAGGACGGC AGAACAGTGT GGTCCTGCAG 
AAACTGAAGC CAGACACTCC TTACACTATC ACCGTATCCT CTCTGTATCC TGATGGTGAA 
GGAGGTCGGA TGACGGGAAG AGGCAAGACC AAACCTCTAA ACACTGTAAG GAACCTGAGA 
GTGTATGACC CTTCTACCAG CACCTTGAAT GTCCGCTGGG ACCATGCAGA GGGAAATCCT 
CGTCAGTACA AGCTCTTCTA TGCACCAGCA GCAGGTGGTC CAGAGGAACT GGTACCAATC 
CCCGGGAATA CCAATTATGC CATTCTTAGG AATCTGCAGC CAGATACCTC ATACACTGTG 
ACTGTAGTTC CCGTTTATAC TGAAGGTGAT GGGGGACGCA CATCAGATAC TGGAAGGACA 
TTGATGAGAG GACTGGCAAG AAATGTCCAA GTATACAATC CTACACCTAA CAGCCTCGAT 
GTTCGCTGGG ACCCTGCTCC AGGACCTGTG CTGCAATATC GCGTTGTGTA TTCTCCTGTG 
GATGGCACAA GACCCTCAGA ATCTATAGTA GTGCCAGGAA ACACGCGCAT GGTGCATCTG 
GAGCGGCTGA TTCCGGACAC ACTCTATTCC GTGAACCTTG TGGCTCTGTA CTCGGATGGA 
GAGGGAAATC CCAGCCCTGC CCAGGGCCGA ACGCTACCAC GCAGTGGACC AAGGAACCTG 
AGAGTCTTTG GTGAAACAAC CAATAGCCTC TCGGTAGCCT GGGATCATGC TGATGGGCCA 
GTTCAGCAGT ACAGGATCAT CTATTCTCCC ACTGTTGGTG ATCCAATTGA TGAATATACC 
ACAGTCCCAG GCAGAAGAAA CAATGTAATA CTGCAGCCCC TGCAACCTGA CACTCCATAT 
AAAATTACTG TTATTGCTGT TTATGAAGAT GGAGATGGTG GCCATCTAAC AGGAAATGGA 
AGAACTGTGG GACTCCTTCC TCCTCAGAAC ATACACATCT CTGACGAATG GTATACAAGA 
TTCAGGGTGT CCTGGGATCC TTCACCTTCT CCAGTTCTTG GATATAAAAT AGTATATAAG 
CCAGTGGGTT CCAATGAGCC CATGGAAGCC TTTGTTGGAG AAATGACATC ATATACCTTA 
CACAATCTCA ATCCCAGCAC CACCTACGAT GTGAATGTTT ATGCTCAATA TGATTCTGGA 
CTCAGTGTCC CCTTGACAGA TCAAGGCACT ACATTATATT TAAATGTAAC AGATCTGAAA 
ACTTACCAGA TTGGGTGGGA TACATTCTGT GTCAAATGGT CACCTCACCG GGCAGCCACC 
TCCTACAGGC TAAAACTAAG CCCTGCAGAT GGAACAAGGG GACAAGAAAT TACAGTGCGT 
GGATCAGAAA CCAGTCACTG CTTCACTGGC CTTTCACCAG ACACTGATTA TGGTGTCACT 
GTTTTTGTGC AGACACCAAA TCTCGAGGGA CCAGGAGTCT CTGTTAAAGA ACATACCACT 
GTGAAACCAA CAGAAGCCCC TACAGAGCCA CCCACACCTC CTCCCCCTCC CACCATTCCA 
CCAGCCCGGG ATGTATGCAA AGGGGCCAAG GCAGATATTG TGTTCTTGAC TGATGCCTCC 
TGGAGCATTG GGGACGATAA TTTTAACAAA GTTGTAAAAT TCATCTTCAA TACTGTGGGA 
GGCTTTGATG AAATCAGTCC TGCTGGGATT CAGGTTTCAT TTGTGCAATA CAGCGATGAG 
GTCAAGTCTG AGTTCAAGCT GAACACGTAC AATGACAAGG CCCTAGCCCT TGGGGCCCTC 
CAGAATATTA GGTACAGAGG AGGAAACACA AGAACAGGCA AGGCCCTCAC GTTTATCAAG 
GAGAAAGTCT TGACTTGGGA GAGCGGCATG AGGAAGAATG TCCCTAAGGT GTTGGTTGTG 
GTCACGGACG GTCGGTCCCA GGATGAGGTC AAGAAGGCGG CTTTGGTCAT CCAGCAGTCA 
GGGTTCAGTG TCTTTGTAGT TGGTGTGGCT GATGTCGACT ACAATGAGCT TGCCAACATT 
GCCAGCAAAC CAAGTGAACG GCACGTGTTC ATTGTGGACG ACTTTGAATC TTTTGAGAAG 
ATCGAAGACA ATCTTATTAC ATTTGTTTGT GAAACTGCCA CTTCAAGTTG TCCTCTCATT 
TATTTGGATG GCTACACCTC ACCAGGTTTT AAAATGCTTG AAGCATACAA CCTGACAGAA 
AAGAATTTTG CTTCTGTACA AGGAGTATCT TTGGAGTCAG GGTCTTTCCC CAGCTACTCA 
GCATACAGGA TTCAGAAGAA TGCGTTTGTG AATCAGCCTA CAGCAGACCT ACACCCAAAT 
GGACTCCCTC CTTCATACAC GATTATATTA TTATTCAGAC TTCTCCCAGA AACTCCCAGT 
GACCCTTTTG CAATTTGGCA AATCACAGAC AGAGACTACA AACCACAAGT TGGAGTGATT 
GCAGATCCTT CTAGCAAGAC GTTATCATTC TTTAACAAGG ATACAAGAGG CGAGGTGCAA 
ACTGTTACAT TTGACACAGA AGAAGTAAAG ACATTATTTT ATGGAAGTTT TCACAAGGTT 
CATATTGTAG TGACCTCAAA AAGTGTTAAG ATTTACATTG ACTGCTATGA AATTATAGAA 
AAAGACATCA AGGAAGCTGG AAATATAACA ACTGATGGTT ATGAAATTCT TGGAAAACTC 
CTTAAAGGGG AAAGGAAATC AGCCGCATTC CAAATCCAGA GTTTTGACAT TGTCTGCAGT 
CCAGTGTGGA CCAGTAGAGA CAGATGCTGT GATATTCCCT CTAGGAGAGA TGAGGGAAAA 
TGCCCTGCTT TTCCAAATTC TTGCACATGT ACACAGGACA GCGTTGGACC TCCAGGACCT 
CCAGGCCCTG CAGGAGGACC TGGTGCTAAA GGTCCCAGAG GTGAAAGAGG TATCAGTGGG 
GCAATTGGGC CCCCTGGTCC TCGTGGAGAC ATAGGTCCTC CAGGCCCCCA GGGTCCTCCA 
GGCCCTCAGG GACCCAATGG ACTCTCTATT CCGGGAGAGC AAGGTCGCCA AGGGATGAAA 
GGTGATGCTG GAGAGCCAGG ACTTCCAGGC CGAACAGGAA CCCCAGGATT ACCTGGCCCA 
CCAGGACCAA TGGGACCTCC AGGAGACAGA GGCTTCACTG GAAAAGACGG TGCAATGGGA 
CCCAGGGGCC CACCAGGGCC GCCGGGAAGC CCAGGCTCCC CAGGAGTCAC AGGACCAAGT 
GGGAAGCCAG GAAAACCTGG AGATCATGGC AGACCAGGTC CATCTGGGTT GAAAGGAGAA 
AAAGGTGATA GGGGAGACAT TGCTTCCCAG AACATGATGC GAGCAGTTGC AAGACAAGTC 
TGTGAACAAT TGATAAGTGG TCAGATGAAC AGATTCAATC AGATGCTGAA TCAGATTCCA 
AATGATTACC AGTCCAGTCG CAACCAGCCA GGCCCGCCGG GTCCACCGGG ACCTCCTGGT 
AGCGCAGGAG CCAGAGGAGA ACCTGGGCCT GGGGGGCGGC CAGGCTTCCC GGGCACACCA 
GGGATGCAGG GACCCCCTGG GGAACGAGGT TTGCCAGGAG AGAAAGGTGA AAGGGGTACT 
GGATCTTCAG GACCTCGGGG GCTGCCTGGC CCCCCAGGTC CACAAGGAGA ATCCAGAACA 
GGTCCACCAG GGTCCACAGG TTCAAGAGGT CCCCCTGGCC CCCCTGGCCG TCCTGGAAAC 
TCAGGTATCC GAGGACCCCC AGGTCCTCCT GGATACTGTG ATTCTTCTCA GTGTGCCAGC 
ATCCCATACA ACGGGCAAGG CTATCCAGAG CCTTACGTGC CCGAGGGCGG GGCCTACCTG 
CCTGAGCGCG AGCCCTTCAT CGTGCCGGTG GAGCCCGAGC GGACAGCGGA ATACGAGGAC 
TACGGCGCAG ACGAGCCTGC AGAGGAGCCT CCTGAGCCCC ACCGGCGCTG GCGGCGCGCC 
CTGCCCCACG GACCCGGGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011533737.1
CDS	311..9394
Translation	MRSRLPPALAALGAALLLSSIEAEVDPPSDLNFKIIDENTVHMSWAKPVDPIVGYRITVDPTTDGPTKEFTLSASTTETLLSELVPETEYVVTITSYDEVEESVPVIGQLTIQTGSSTKPVEKKPGKTEIQKCSVSAWTDLVFLVDGSWSVGRNNFKYILDFIAALVSAFDIGEEKTRVGVVQYSSDTRTEFNLNQYYQRDELLAAIKKIPYKGGNTMTGDAIDYLVKNTFTESAGARVGFPKVAIIITDGKSQDEVEIPARELRNVGVEVFSLGIKAADAKELKQIASTPSLNHVFNVANFDAIVDIQNEIISQVCSGVDEQLGELVSGEEVVEPPSNLIAMEVSSKYVKLNWNPSPSPVTGYKVILTPMTAGSRQHALSVGPQTTTLSVRDLSADTEYQISVSAMKGMTSSEPISIMEKTQPMKVQVECSRGVDIKADIVFLVDGSYSIGIANFVKVRAFLEVLVKSFEISPNRVQISLVQYSRDPHTEFTLKKFTKVEDIIEAINTFPYRGGSTNTGKAMTYVREKIFVPSKGSRSNVPKVMILITDGKSSDAFRDPAIKLRNSDVEIFAVGVKDAVRSELEAIASPPAETHVFTVEDFDAFQRISFELTQSICLRIEQELAAIKKKAYVPPKDLSFSEVTSYGFKTNWSPAGENVFSYHITYKEAAGDDEVTVVEPASSTSVVLSSLKPETLYLVNVTAEYEDGFSIPLAGEETTEEVKGAPRNLKVTDETTDSFKITWTQAPGRVLRYRIIYRPVAGGESREVTTPPNQRRRTLENLIPDTKYEVSVIPEYFSGPGTPLTGNAATEEERGSPQDLVTKDITDTSIGAYWTSAPGMVRGYRVSWKSLYDDVDTGEKNLPEDAIHTMIENLQPETKYRISVFATYSSGEGEPLTGDATTELSQDSKTLKVDEETENTMRVTWKPAPGKVVNYRVVYRPHGRGKQMVAKVPPTVTSTVLKRLQPQTTYDITVLPIYKMGEGKLRQGSGTTASRFKSPRNLKTSDPTMSSFRVTWEPAPGEVKGYKVTFHPTGDDRRLGELVVGPYDNTVVLEELRAGTTYKVNVFGMFDGGESSPLVGQEMTTLSDTTVMPILSSGMECLTRAEADIVLLVDGSWSIGRANFRTVRSFISRIVEVFDIGPKRVQIALAQYSGDPRTEWQLNAHRDKKSLLQAVANLPYKGGNTLTGMALNFIRQQNFRTQAGMRPRARKIGVLITDGKSQDDVEAPSKKLKDEGVELFAIGIKNADEVELKMIATDPDDTHAYNVADFESLSRIVDDLTINLCNSVKGPGDLEAPSNLVISERTHRSFRVSWTPPSDSVDRYKVEYYPVSGGKRQEFYVSRMETSTVLKDLKPETEYVVNVYSVVEDEYSEPLKGTEKTLPVPVVSLNIYDVGPTTMHVQWQPVGGATGYILSYKPVKDTEPTRPKEVRLGPTVNDMQLTDLVPNTEYAVTVQAVLHDLTSEPVTVREVTLPLPRPQDLKLRDVTHSTMNVFWEPVPGKVRKYIVRYKTPEEDVKEVEVDRSETSTSLKDLFSQTLYTVSVSAVHDEGESPPVTAQETTRPVPAPTNLKITEVTSEGFRGTWDHGASDVSLYRITWAPFGSSDKMETILNGDENTLVFENLNPNTIYEVSITAIYPDESESDDLIGSERTLPILTTQAPKSGPRNLQVYNATSNSLTVKWDPASGRVQKYRITYQPSTGEGNEQTTTIGGRQNSVVLQKLKPDTPYTITVSSLYPDGEGGRMTGRGKTKPLNTVRNLRVYDPSTSTLNVRWDHAEGNPRQYKLFYAPAAGGPEELVPIPGNTNYAILRNLQPDTSYTVTVVPVYTEGDGGRTSDTGRTLMRGLARNVQVYNPTPNSLDVRWDPAPGPVLQYRVVYSPVDGTRPSESIVVPGNTRMVHLERLIPDTLYSVNLVALYSDGEGNPSPAQGRTLPRSGPRNLRVFGETTNSLSVAWDHADGPVQQYRIIYSPTVGDPIDEYTTVPGRRNNVILQPLQPDTPYKITVIAVYEDGDGGHLTGNGRTVGLLPPQNIHISDEWYTRFRVSWDPSPSPVLGYKIVYKPVGSNEPMEAFVGEMTSYTLHNLNPSTTYDVNVYAQYDSGLSVPLTDQGTTLYLNVTDLKTYQIGWDTFCVKWSPHRAATSYRLKLSPADGTRGQEITVRGSETSHCFTGLSPDTDYGVTVFVQTPNLEGPGVSVKEHTTVKPTEAPTEPPTPPPPPTIPPARDVCKGAKADIVFLTDASWSIGDDNFNKVVKFIFNTVGGFDEISPAGIQVSFVQYSDEVKSEFKLNTYNDKALALGALQNIRYRGGNTRTGKALTFIKEKVLTWESGMRKNVPKVLVVVTDGRSQDEVKKAALVIQQSGFSVFVVGVADVDYNELANIASKPSERHVFIVDDFESFEKIEDNLITFVCETATSSCPLIYLDGYTSPGFKMLEAYNLTEKNFASVQGVSLESGSFPSYSAYRIQKNAFVNQPTADLHPNGLPPSYTIILLFRLLPETPSDPFAIWQITDRDYKPQVGVIADPSSKTLSFFNKDTRGEVQTVTFDTEEVKTLFYGSFHKVHIVVTSKSVKIYIDCYEIIEKDIKEAGNITTDGYEILGKLLKGERKSAAFQIQSFDIVCSPVWTSRDRCCDIPSRRDEGKCPAFPNSCTCTQDSVGPPGPPGPAGGPGAKGPRGERGISGAIGPPGPRGDIGPPGPQGPPGPQGPNGLSIPGEQGRQGMKGDAGEPGLPGRTGTPGLPGPPGPMGPPGDRGFTGKDGAMGPRGPPGPPGSPGSPGVTGPSGKPGKPGDHGRPGPSGLKGEKGDRGDIASQNMMRAVARQVCEQLISGQMNRFNQMLNQIPNDYQSSRNQPGPPGPPGPPGSAGARGEPGPGGRPGFPGTPGMQGPPGERGLPGEKGERGTGSSGPRGLPGPPGPQGESRTGPPGSTGSRGPPGPPGRPGNSGIRGPPGPPGYCDSSQCASIPYNGQGYPEPYVPEGGAYLPEREPFIVPVEPERTAEYEDYGADEPAEEPPEPHRRWRRALPHGPGQ

Target ORF information:

RefSeq Version	XM_011535435.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XII alpha 1 chain (COL12A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535435.1

ORF Insert Sequence:

ATGCGGAGTA GGCTTCCCCC AGCGCTTGCC GCCCTGGGCG CGGCCCTGCT CCTGTCTTCC 
ATTGAGGCAG AAGTTGACCC ACCTTCAGAC TTGAATTTTA AAATTATAGA TGAAAATACT 
GTTCATATGT CATGGGCAAA ACCAGTTGAT CCAATTGTGG GTTACAGAAT AACGGTGGAC 
CCTACAACGG ATGGGCCTAC TAAAGAATTT ACCCTTTCAG CTAGTACCAC TGAAACTTTA 
TTGTCAGAAC TTGTACCTGA AACAGAGTAT GTGGTGACAA TAACTTCATA TGATGAAGTA 
GAAGAAAGTG TACCAGTTAT AGGACAACTA ACAATTCAAA CAGGTAGTTC GACAAAGCCA 
GTGGAGAAGA AACCTGGAAA AACCGAGATA CAAAAATGCT CTGTCAGTGC CTGGACTGAT 
TTGGTTTTCC TCGTGGATGG CTCTTGGAGT GTGGGAAGAA ATAATTTCAA GTACATTTTA 
GACTTCATTG CTGCTCTTGT GTCTGCTTTT GACATTGGGG AAGAGAAGAC AAGAGTTGGA 
GTTGTTCAAT ACAGCTCTGA TACCAGGACT GAATTTAACT TAAATCAGTA CTACCAAAGG 
GATGAACTTC TTGCTGCAAT AAAAAAAATT CCATATAAAG GTGGCAACAC AATGACAGGG 
GATGCCATTG ATTATTTAGT TAAAAATACT TTCACGGAAT CTGCTGGGGC AAGAGTTGGC 
TTTCCTAAAG TGGCAATTAT TATTACGGAT GGAAAATCCC AGGATGAAGT GGAAATTCCA 
GCAAGAGAGC TTCGTAATGT TGGAGTTGAA GTTTTCTCCT TGGGCATTAA AGCTGCAGAT 
GCAAAAGAAC TCAAACAAAT TGCCTCCACA CCTTCACTGA ACCATGTTTT CAATGTGGCC 
AACTTTGATG CAATTGTGGA TATTCAGAAT GAGATCATCT CCCAGGTGTG CTCAGGTGTT 
GATGAACAAC TTGGTGAATT GGTTAGTGGA GAAGAAGTTG TTGAGCCTCC TTCAAATTTG 
ATTGCCATGG AAGTCTCTTC AAAATATGTT AAGCTAAATT GGAATCCATC TCCTAGTCCA 
GTGACTGGCT ACAAAGTCAT CCTCACACCA ATGACTGCAG GAAGCCGACA GCACGCTCTG 
AGTGTGGGGC CTCAGACAAC CACGCTCAGT GTTCGCGACC TCTCAGCAGA CACAGAATAC 
CAGATCAGTG TTTCCGCCAT GAAGGGAATG ACATCCAGTG AACCCATTTC AATAATGGAG 
AAGACTCAGC CAATGAAAGT TCAAGTGGAA TGCTCACGTG GTGTGGATAT AAAAGCCGAT 
ATTGTGTTTT TGGTTGATGG CTCCTATAGC ATTGGGATTG CAAACTTTGT TAAAGTTAGA 
GCCTTTTTGG AAGTTCTTGT AAAAAGTTTT GAAATTTCAC CAAATAGGGT CCAGATTAGT 
CTTGTGCAAT ACAGCCGGGA TCCTCATACT GAGTTCACTT TGAAAAAATT CACCAAAGTT 
GAAGATATAA TTGAAGCAAT AAACACCTTC CCTTACAGAG GAGGATCTAC AAATACTGGC 
AAAGCAATGA CTTATGTCAG AGAGAAAATA TTTGTGCCTA GCAAGGGATC AAGAAGCAAT 
GTGCCAAAGG TCATGATTCT TATCACGGAT GGGAAATCAT CAGATGCTTT CAGAGATCCT 
GCGATAAAAC TGAGGAATTC AGATGTTGAA ATCTTTGCAG TTGGTGTGAA GGATGCCGTT 
CGCTCAGAAT TGGAAGCTAT TGCCTCTCCT CCTGCAGAGA CCCATGTGTT CACAGTGGAA 
GATTTTGATG CTTTTCAGAG GATATCTTTT GAACTCACAC AGTCTATCTG CCTTAGAATT 
GAGCAAGAAT TGGCAGCTAT AAAGAAGAAA GCTTACGTCC CTCCAAAGGA TCTTAGTTTT 
TCAGAAGTGA CTTCTTATGG TTTCAAAACC AACTGGTCTC CAGCTGGAGA AAATGTTTTT 
TCATATCACA TCACCTACAA GGAAGCGGCT GGGGATGATG AGGTCACTGT GGTGGAGCCA 
GCATCGAGCA CCAGTGTTGT TCTCAGCAGC CTGAAGCCAG AGACCTTGTA TTTGGTCAAT 
GTGACTGCGG AGTATGAGGA TGGCTTCAGC ATTCCCTTAG CTGGAGAGGA GACCACCGAA 
GAAGTAAAAG GAGCACCTCG AAACCTAAAG GTGACAGATG AGACTACAGA TAGTTTCAAA 
ATTACTTGGA CTCAAGCTCC AGGGAGAGTT TTAAGATATC GAATTATATA TAGACCAGTT 
GCTGGTGGAG AGAGCAGAGA AGTTACCACC CCACCCAATC AGAGGAGGAG AACACTGGAG 
AACTTGATTC CAGACACGAA ATATGAAGTA TCTGTAATTC CTGAATATTT CTCAGGACCT 
GGTACTCCAT TAACTGGAAA TGCAGCCACT GAAGAAGAAC GTGGTTCTCC TCAAGATTTA 
GTTACTAAAG ACATCACTGA CACATCAATT GGGGCTTATT GGACATCTGC TCCAGGAATG 
GTTCGCGGTT ACAGGGTCTC ATGGAAATCA CTTTATGATG ATGTTGACAC TGGAGAGAAA 
AATCTGCCTG AAGATGCAAT TCATACGATG ATAGAAAATC TGCAGCCAGA GACCAAATAC 
AGAATTTCAG TATTTGCCAC TTACAGCAGT GGAGAAGGAG AACCTTTGAC TGGAGATGCC 
ACAACTGAAT TATCTCAAGA TTCCAAAACC CTGAAAGTAG ATGAAGAAAC AGAAAACACA 
ATGAGAGTTA CATGGAAACC AGCACCAGGG AAAGTCGTCA ACTACCGTGT TGTCTATCGC 
CCTCATGGGA GAGGGAAGCA AATGGTTGCT AAGGTGCCCC CCACAGTCAC TTCGACAGTG 
TTAAAGCGAC TTCAGCCACA GACCACATAT GACATCACAG TTCTTCCTAT TTACAAGATG 
GGAGAAGGAA AGCTTAGGCA AGGATCAGGA ACAACAGCTT CTCGGTTTAA GTCTCCTAGA 
AACCTCAAAA CATCTGACCC AACCATGTCA AGCTTCCGAG TGACTTGGGA GCCTGCCCCT 
GGGGAAGTGA AGGGTTATAA AGTCACATTC CACCCTACGG GGGATGACAG AAGACTGGGG 
GAGTTAGTGG TTGGACCCTA TGACAACACA GTTGTTTTGG AGGAACTTAG GGCTGGTACC 
ACCTATAAAG TAAATGTTTT TGGAATGTTT GATGGAGGAG AAAGCTCACC ACTTGTTGGA 
CAAGAAATGA CAACCCTTTC CGACACAACT GTTATGCCAA TTTTATCTTC TGGGATGGAG 
TGTCTCACCA GAGCTGAGGC AGACATTGTG TTGCTGGTGG ATGGATCATG GAGCATCGGC 
CGGGCAAATT TTAGAACCGT GAGGAGTTTC ATTTCTCGTA TTGTGGAAGT CTTTGACATT 
GGCCCCAAAA GAGTACAAAT TGCTCTTGCT CAGTATAGTG GGGATCCCAG AACAGAGTGG 
CAGTTAAATG CACACAGAGA CAAGAAGAGC TTGTTGCAAG CTGTGGCAAA CTTGCCGTAC 
AAAGGAGGCA ATACTCTCAC AGGCATGGCT TTGAATTTCA TTCGCCAACA GAACTTCAGG 
ACCCAAGCTG GCATGAGACC TCGAGCTCGA AAAATTGGTG TGCTCATTAC TGATGGAAAA 
TCACAAGACG ATGTTGAAGC ACCTTCAAAG AAACTCAAGG ATGAGGGAGT GGAGCTGTTT 
GCTATTGGTA TTAAAAATGC TGATGAAGTC GAATTAAAGA TGATTGCAAC TGATCCTGAT 
GATACCCATG CATACAATGT GGCAGATTTT GAGTCACTCT CCAGGATAGT GGATGATCTC 
ACCATTAATT TGTGTAACAG TGTCAAAGGT CCAGGTGATT TGGAAGCACC TTCTAACTTA 
GTTATTTCTG AGCGAACCCA TCGTTCTTTT AGAGTGAGCT GGACACCACC TTCTGACAGT 
GTGGATCGAT ATAAGGTGGA ATACTATCCA GTTTCTGGAG GGAAACGTCA AGAATTTTAT 
GTGAGTCGAA TGGAAACTAG CACAGTGCTG AAAGATCTGA AACCTGAAAC TGAATATGTT 
GTCAATGTGT ATTCTGTGGT AGAAGATGAA TATAGTGAGC CTCTGAAGGG GACAGAAAAA 
ACCTTGCCAG TGCCTGTAGT CAGCCTGAAT ATTTATGATG TTGGCCCTAC CACCATGCAT 
GTGCAGTGGC AGCCTGTGGG AGGAGCTACT GGCTACATCT TGTCATACAA ACCTGTTAAG 
GACACAGAGC CAACAAGACC CAAAGAGGTG CGTTTGGGGC CAACAGTGAA TGACATGCAG 
CTGACTGACC TTGTTCCCAA CACGGAGTAT GCAGTCACAG TCCAGGCTGT CCTGCACGAC 
CTCACTAGTG AACCTGTCAC TGTTCGGGAA GTCACCTTGC CTTTACCCAG ACCTCAGGAT 
CTGAAACTCA GAGATGTGAC TCACAGCACT ATGAATGTCT TTTGGGAACC TGTGCCTGGA 
AAAGTGCGTA AATATATTGT TCGATACAAA ACACCAGAAG AGGATGTCAA AGAGGTAGAG 
GTGGACAGAT CAGAGACCAG CACTTCCCTC AAAGACCTCT TCTCACAGAC CTTGTACACA 
GTCAGCGTTT CTGCAGTACA TGACGAGGGG GAGTCTCCTC CAGTGACTGC TCAAGAAACT 
ACCCGACCCG TGCCAGCCCC AACAAACTTA AAGATTACTG AAGTAACATC AGAGGGTTTC 
AGAGGGACTT GGGATCATGG AGCTTCAGAT GTGTCTCTCT ACAGAATAAC TTGGGCACCT 
TTTGGAAGCT CAGATAAGAT GGAGACCATC TTAAATGGAG ATGAAAACAC TTTGGTGTTC 
GAAAACCTGA ACCCCAACAC CATCTATGAA GTTTCCATTA CTGCCATCTA TCCTGATGAG 
TCAGAAAGTG ATGACCTGAT TGGCAGTGAG CGCACACTGC CTATCTTAAC AACACAAGCT 
CCCAAAAGTG GCCCACGAAA CCTTCAAGTG TACAATGCAA CATCTAACAG CCTGACTGTT 
AAGTGGGATC CTGCTAGTGG TCGTGTGCAG AAATATAGGA TCACTTATCA GCCTTCCACA 
GGGGAAGGCA ATGAGCAAAC GACCACAATA GGAGGACGGC AGAACAGTGT GGTCCTGCAG 
AAACTGAAGC CAGACACTCC TTACACTATC ACCGTATCCT CTCTGTATCC TGATGGTGAA 
GGAGGTCGGA TGACGGGAAG AGGCAAGACC AAACCTCTAA ACACTGTAAG GAACCTGAGA 
GTGTATGACC CTTCTACCAG CACCTTGAAT GTCCGCTGGG ACCATGCAGA GGGAAATCCT 
CGTCAGTACA AGCTCTTCTA TGCACCAGCA GCAGGTGGTC CAGAGGAACT GGTACCAATC 
CCCGGGAATA CCAATTATGC CATTCTTAGG AATCTGCAGC CAGATACCTC ATACACTGTG 
ACTGTAGTTC CCGTTTATAC TGAAGGTGAT GGGGGACGCA CATCAGATAC TGGAAGGACA 
TTGATGAGAG GACTGGCAAG AAATGTCCAA GTATACAATC CTACACCTAA CAGCCTCGAT 
GTTCGCTGGG ACCCTGCTCC AGGACCTGTG CTGCAATATC GCGTTGTGTA TTCTCCTGTG 
GATGGCACAA GACCCTCAGA ATCTATAGTA GTGCCAGGAA ACACGCGCAT GGTGCATCTG 
GAGCGGCTGA TTCCGGACAC ACTCTATTCC GTGAACCTTG TGGCTCTGTA CTCGGATGGA 
GAGGGAAATC CCAGCCCTGC CCAGGGCCGA ACGCTACCAC GCAGTGGACC AAGGAACCTG 
AGAGTCTTTG GTGAAACAAC CAATAGCCTC TCGGTAGCCT GGGATCATGC TGATGGGCCA 
GTTCAGCAGT ACAGGATCAT CTATTCTCCC ACTGTTGGTG ATCCAATTGA TGAATATACC 
ACAGTCCCAG GCAGAAGAAA CAATGTAATA CTGCAGCCCC TGCAACCTGA CACTCCATAT 
AAAATTACTG TTATTGCTGT TTATGAAGAT GGAGATGGTG GCCATCTAAC AGGAAATGGA 
AGAACTGTGG GACTCCTTCC TCCTCAGAAC ATACACATCT CTGACGAATG GTATACAAGA 
TTCAGGGTGT CCTGGGATCC TTCACCTTCT CCAGTTCTTG GATATAAAAT AGTATATAAG 
CCAGTGGGTT CCAATGAGCC CATGGAAGCC TTTGTTGGAG AAATGACATC ATATACCTTA 
CACAATCTCA ATCCCAGCAC CACCTACGAT GTGAATGTTT ATGCTCAATA TGATTCTGGA 
CTCAGTGTCC CCTTGACAGA TCAAGGCACT ACATTATATT TAAATGTAAC AGATCTGAAA 
ACTTACCAGA TTGGGTGGGA TACATTCTGT GTCAAATGGT CACCTCACCG GGCAGCCACC 
TCCTACAGGC TAAAACTAAG CCCTGCAGAT GGAACAAGGG GACAAGAAAT TACAGTGCGT 
GGATCAGAAA CCAGTCACTG CTTCACTGGC CTTTCACCAG ACACTGATTA TGGTGTCACT 
GTTTTTGTGC AGACACCAAA TCTCGAGGGA CCAGGAGTCT CTGTTAAAGA ACATACCACT 
GTGAAACCAA CAGAAGCCCC TACAGAGCCA CCCACACCTC CTCCCCCTCC CACCATTCCA 
CCAGCCCGGG ATGTATGCAA AGGGGCCAAG GCAGATATTG TGTTCTTGAC TGATGCCTCC 
TGGAGCATTG GGGACGATAA TTTTAACAAA GTTGTAAAAT TCATCTTCAA TACTGTGGGA 
GGCTTTGATG AAATCAGTCC TGCTGGGATT CAGGTTTCAT TTGTGCAATA CAGCGATGAG 
GTCAAGTCTG AGTTCAAGCT GAACACGTAC AATGACAAGG CCCTAGCCCT TGGGGCCCTC 
CAGAATATTA GGTACAGAGG AGGAAACACA AGAACAGGCA AGGCCCTCAC GTTTATCAAG 
GAGAAAGTCT TGACTTGGGA GAGCGGCATG AGGAAGAATG TCCCTAAGGT GTTGGTTGTG 
GTCACGGACG GTCGGTCCCA GGATGAGGTC AAGAAGGCGG CTTTGGTCAT CCAGCAGTCA 
GGGTTCAGTG TCTTTGTAGT TGGTGTGGCT GATGTCGACT ACAATGAGCT TGCCAACATT 
GCCAGCAAAC CAAGTGAACG GCACGTGTTC ATTGTGGACG ACTTTGAATC TTTTGAGAAG 
ATCGAAGACA ATCTTATTAC ATTTGTTTGT GAAACTGCCA CTTCAAGTTG TCCTCTCATT 
TATTTGGATG GCTACACCTC ACCAGGTTTT AAAATGCTTG AAGCATACAA CCTGACAGAA 
AAGAATTTTG CTTCTGTACA AGGAGTATCT TTGGAGTCAG GGTCTTTCCC CAGCTACTCA 
GCATACAGGA TTCAGAAGAA TGCGTTTGTG AATCAGCCTA CAGCAGACCT ACACCCAAAT 
GGACTCCCTC CTTCATACAC GATTATATTA TTATTCAGAC TTCTCCCAGA AACTCCCAGT 
GACCCTTTTG CAATTTGGCA AATCACAGAC AGAGACTACA AACCACAAGT TGGAGTGATT 
GCAGATCCTT CTAGCAAGAC GTTATCATTC TTTAACAAGG ATACAAGAGG CGAGGTGCAA 
ACTGTTACAT TTGACACAGA AGAAGTAAAG ACATTATTTT ATGGAAGTTT TCACAAGGTT 
CATATTGTAG TGACCTCAAA AAGTGTTAAG ATTTACATTG ACTGCTATGA AATTATAGAA 
AAAGACATCA AGGAAGCTGG AAATATAACA ACTGATGGTT ATGAAATTCT TGGAAAACTC 
CTTAAAGGGG AAAGGAAATC AGCCGCATTC CAAATCCAGA GTTTTGACAT TGTCTGCAGT 
CCAGTGTGGA CCAGTAGAGA CAGATGCTGT GATATTCCCT CTAGGAGAGA TGAGGGAAAA 
TGCCCTGCTT TTCCAAATTC TTGCACATGT ACACAGGACA GCGTTGGACC TCCAGGACCT 
CCAGGCCCTG CAGGAGGACC TGGTGCTAAA GGTCCCAGAG GTGAAAGAGG TATCAGTGGG 
GCAATTGGGC CCCCTGGTCC TCGTGGAGAC ATAGGTCCTC CAGGCCCCCA GGGTCCTCCA 
GGCCCTCAGG GACCCAATGG ACTCTCTATT CCGGGAGAGC AAGGTCGCCA AGGGATGAAA 
GGTGATGCTG GAGAGCCAGG ACTTCCAGGC CGAACAGGAA CCCCAGGATT ACCTGGCCCA 
CCAGGACCAA TGGGACCTCC AGGAGACAGA GGCTTCACTG GAAAAGACGG TGCAATGGGA 
CCCAGGGGCC CACCAGGGCC GCCGGGAAGC CCAGGCTCCC CAGGAGTCAC AGGACCAAGT 
GGGAAGCCAG GAAAACCTGG AGATCATGGC AGACCAGGTC CATCTGGGTT GAAAGGAGAA 
AAAGGTGATA GGGGAGACAT TGCTTCCCAG AACATGATGC GAGCAGTTGC AAGACAAGTC 
TGTGAACAAT TGATAAGTGG TCAGATGAAC AGATTCAATC AGATGCTGAA TCAGATTCCA 
AATGATTACC AGTCCAGTCG CAACCAGCCA GGCCCGCCGG GTCCACCGGG ACCTCCTGGT 
AGCGCAGGAG CCAGAGGAGA ACCTGGGCCT GGGGGGCGGC CAGGCTTCCC GGGCACACCA 
GGGATGCAGG GACCCCCTGG GGAACGAGGT TTGCCAGGAG AGAAAGGTGA AAGGGGTACT 
GGATCTTCAG GACCTCGGGG GCTGCCTGGC CCCCCAGGTC CACAAGGAGA ATCCAGAACA 
GGTCCACCAG GGTCCACAGG TTCAAGAGGT CCCCCTGGCC CCCCTGGCCG TCCTGGAAAC 
TCAGGTATCC GAGGACCCCC AGGTCCTCCT GGATACTGTG ATTCTTCTCA GTGTGCCAGC 
ATCCCATACA ACGGGCAAGG CTATCCAGAG CCTTACGTGC CCGAGGGCGG GGCCTACCTG 
CCTGAGCGCG AGCCCTTCAT CGTGCCGGTG GAGCCCGAGC GGACAGCGGA ATACGAGGAC 
TACGGCGCAG ACGAGCCTGC AGAGGAGCCT CCTGAGCCCC ACCGGCGCTG GCGGCGCGCC 
CTGCCCCACG GACCCGGGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL12A1 ( XM_011535436.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011533738.1 Homo sapiens collagen type XII alpha 1 chain (COL12A1), transcript variant X4, mRNA.

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CloneID	OHu57024
Clone ID Related Accession (Same CDS sequence)	XM_011535436.2
Accession Version	XM_011535436.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(XII) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535436.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGTA GGCTTCCCCC AGCGCTTGCC GCCCTGGGCG CGGCCCTGCT CCTGTCTTCC 
ATTGAGGCAG AAGGGATGGA GTGTCTCACC AGAGCTGAGG CAGACATTGT GTTGCTGGTG 
GATGGATCAT GGAGCATCGG CCGGGCAAAT TTTAGAACCG TGAGGAGTTT CATTTCTCGT 
ATTGTGGAAG TCTTTGACAT TGGCCCCAAA AGAGTACAAA TTGCTCTTGC TCAGTATAGT 
GGGGATCCCA GAACAGAGTG GCAGTTAAAT GCACACAGAG ACAAGAAGAG CTTGTTGCAA 
GCTGTGGCAA ACTTGCCGTA CAAAGGAGGC AATACTCTCA CAGGCATGGC TTTGAATTTC 
ATTCGCCAAC AGAACTTCAG GACCCAAGCT GGCATGAGAC CTCGAGCTCG AAAAATTGGT 
GTGCTCATTA CTGATGGAAA ATCACAAGAC GATGTTGAAG CACCTTCAAA GAAACTCAAG 
GATGAGGGAG TGGAGCTGTT TGCTATTGGT ATTAAAAATG CTGATGAAGT CGAATTAAAG 
ATGATTGCAA CTGATCCTGA TGATACCCAT GCATACAATG TGGCAGATTT TGAGTCACTC 
TCCAGGATAG TGGATGATCT CACCATTAAT TTGTGTAACA GTGTCAAAGG TCCAGGTGAT 
TTGGAAGCAC CTTCTAACTT AGTTATTTCT GAGCGAACCC ATCGTTCTTT TAGAGTGAGC 
TGGACACCAC CTTCTGACAG TGTGGATCGA TATAAGGTGG AATACTATCC AGTTTCTGGA 
GGGAAACGTC AAGAATTTTA TGTGAGTCGA ATGGAAACTA GCACAGTGCT GAAAGATCTG 
AAACCTGAAA CTGAATATGT TGTCAATGTG TATTCTGTGG TAGAAGATGA ATATAGTGAG 
CCTCTGAAGG GGACAGAAAA AACCTTGCCA GTGCCTGTAG TCAGCCTGAA TATTTATGAT 
GTTGGCCCTA CCACCATGCA TGTGCAGTGG CAGCCTGTGG GAGGAGCTAC TGGCTACATC 
TTGTCATACA AACCTGTTAA GGACACAGAG CCAACAAGAC CCAAAGAGGT GCGTTTGGGG 
CCAACAGTGA ATGACATGCA GCTGACTGAC CTTGTTCCCA ACACGGAGTA TGCAGTCACA 
GTCCAGGCTG TCCTGCACGA CCTCACTAGT GAACCTGTCA CTGTTCGGGA AGTCACCTTG 
CCTTTACCCA GACCTCAGGA TCTGAAACTC AGAGATGTGA CTCACAGCAC TATGAATGTC 
TTTTGGGAAC CTGTGCCTGG AAAAGTGCGT AAATATATTG TTCGATACAA AACACCAGAA 
GAGGATGTCA AAGAGGTAGA GGTGGACAGA TCAGAGACCA GCACTTCCCT CAAAGACCTC 
TTCTCACAGA CCTTGTACAC AGTCAGCGTT TCTGCAGTAC ATGACGAGGG GGAGTCTCCT 
CCAGTGACTG CTCAAGAAAC TACCCGACCC GTGCCAGCCC CAACAAACTT AAAGATTACT 
GAAGTAACAT CAGAGGGTTT CAGAGGGACT TGGGATCATG GAGCTTCAGA TGTGTCTCTC 
TACAGAATAA CTTGGGCACC TTTTGGAAGC TCAGATAAGA TGGAGACCAT CTTAAATGGA 
GATGAAAACA CTTTGGTGTT CGAAAACCTG AACCCCAACA CCATCTATGA AGTTTCCATT 
ACTGCCATCT ATCCTGATGA GTCAGAAAGT GATGACCTGA TTGGCAGTGA GCGCACACTG 
CCTATCTTAA CAACACAAGC TCCCAAAAGT GGCCCACGAA ACCTTCAAGT GTACAATGCA 
ACATCTAACA GCCTGACTGT TAAGTGGGAT CCTGCTAGTG GTCGTGTGCA GAAATATAGG 
ATCACTTATC AGCCTTCCAC AGGGGAAGGC AATGAGCAAA CGACCACAAT AGGAGGACGG 
CAGAACAGTG TGGTCCTGCA GAAACTGAAG CCAGACACTC CTTACACTAT CACCGTATCC 
TCTCTGTATC CTGATGGTGA AGGAGGTCGG ATGACGGGAA GAGGCAAGAC CAAACCTCTA 
AACACTGTAA GGAACCTGAG AGTGTATGAC CCTTCTACCA GCACCTTGAA TGTCCGCTGG 
GACCATGCAG AGGGAAATCC TCGTCAGTAC AAGCTCTTCT ATGCACCAGC AGCAGGTGGT 
CCAGAGGAAC TGGTACCAAT CCCCGGGAAT ACCAATTATG CCATTCTTAG GAATCTGCAG 
CCAGATACCT CATACACTGT GACTGTAGTT CCCGTTTATA CTGAAGGTGA TGGGGGACGC 
ACATCAGATA CTGGAAGGAC ATTGATGAGA GGACTGGCAA GAAATGTCCA AGTATACAAT 
CCTACACCTA ACAGCCTCGA TGTTCGCTGG GACCCTGCTC CAGGACCTGT GCTGCAATAT 
CGCGTTGTGT ATTCTCCTGT GGATGGCACA AGACCCTCAG AATCTATAGT AGTGCCAGGA 
AACACGCGCA TGGTGCATCT GGAGCGGCTG ATTCCGGACA CACTCTATTC CGTGAACCTT 
GTGGCTCTGT ACTCGGATGG AGAGGGAAAT CCCAGCCCTG CCCAGGGCCG AACGCTACCA 
CGCAGTGGAC CAAGGAACCT GAGAGTCTTT GGTGAAACAA CCAATAGCCT CTCGGTAGCC 
TGGGATCATG CTGATGGGCC AGTTCAGCAG TACAGGATCA TCTATTCTCC CACTGTTGGT 
GATCCAATTG ATGAATATAC CACAGTCCCA GGCAGAAGAA ACAATGTAAT ACTGCAGCCC 
CTGCAACCTG ACACTCCATA TAAAATTACT GTTATTGCTG TTTATGAAGA TGGAGATGGT 
GGCCATCTAA CAGGAAATGG AAGAACTGTG GGACTCCTTC CTCCTCAGAA CATACACATC 
TCTGACGAAT GGTATACAAG ATTCAGGGTG TCCTGGGATC CTTCACCTTC TCCAGTTCTT 
GGATATAAAA TAGTATATAA GCCAGTGGGT TCCAATGAGC CCATGGAAGC CTTTGTTGGA 
GAAATGACAT CATATACCTT ACACAATCTC AATCCCAGCA CCACCTACGA TGTGAATGTT 
TATGCTCAAT ATGATTCTGG ACTCAGTGTC CCCTTGACAG ATCAAGGCAC TACATTATAT 
TTAAATGTAA CAGATCTGAA AACTTACCAG ATTGGGTGGG ATACATTCTG TGTCAAATGG 
TCACCTCACC GGGCAGCCAC CTCCTACAGG CTAAAACTAA GCCCTGCAGA TGGAACAAGG 
GGACAAGAAA TTACAGTGCG TGGATCAGAA ACCAGTCACT GCTTCACTGG CCTTTCACCA 
GACACTGATT ATGGTGTCAC TGTTTTTGTG CAGACACCAA ATCTCGAGGG ACCAGGAGTC 
TCTGTTAAAG AACATACCAC TGTGAAACCA ACAGAAGCCC CTACAGAGCC ACCCACACCT 
CCTCCCCCTC CCACCATTCC ACCAGCCCGG GATGTATGCA AAGGGGCCAA GGCAGATATT 
GTGTTCTTGA CTGATGCCTC CTGGAGCATT GGGGACGATA ATTTTAACAA AGTTGTAAAA 
TTCATCTTCA ATACTGTGGG AGGCTTTGAT GAAATCAGTC CTGCTGGGAT TCAGGTTTCA 
TTTGTGCAAT ACAGCGATGA GGTCAAGTCT GAGTTCAAGC TGAACACGTA CAATGACAAG 
GCCCTAGCCC TTGGGGCCCT CCAGAATATT AGGTACAGAG GAGGAAACAC AAGAACAGGC 
AAGGCCCTCA CGTTTATCAA GGAGAAAGTC TTGACTTGGG AGAGCGGCAT GAGGAAGAAT 
GTCCCTAAGG TGTTGGTTGT GGTCACGGAC GGTCGGTCCC AGGATGAGGT CAAGAAGGCG 
GCTTTGGTCA TCCAGCAGTC AGGGTTCAGT GTCTTTGTAG TTGGTGTGGC TGATGTCGAC 
TACAATGAGC TTGCCAACAT TGCCAGCAAA CCAAGTGAAC GGCACGTGTT CATTGTGGAC 
GACTTTGAAT CTTTTGAGAA GATCGAAGAC AATCTTATTA CATTTGTTTG TGAAACTGCC 
ACTTCAAGTT GTCCTCTCAT TTATTTGGAT GGCTACACCT CACCAGGTTT TAAAATGCTT 
GAAGCATACA ACCTGACAGA AAAGAATTTT GCTTCTGTAC AAGGAGTATC TTTGGAGTCA 
GGGTCTTTCC CCAGCTACTC AGCATACAGG ATTCAGAAGA ATGCGTTTGT GAATCAGCCT 
ACAGCAGACC TACACCCAAA TGGACTCCCT CCTTCATACA CGATTATATT ATTATTCAGA 
CTTCTCCCAG AAACTCCCAG TGACCCTTTT GCAATTTGGC AAATCACAGA CAGAGACTAC 
AAACCACAAG TTGGAGTGAT TGCAGATCCT TCTAGCAAGA CGTTATCATT CTTTAACAAG 
GATACAAGAG GCGAGGTGCA AACTGTTACA TTTGACACAG AAGAAGTAAA GACATTATTT 
TATGGAAGTT TTCACAAGGT TCATATTGTA GTGACCTCAA AAAGTGTTAA GATTTACATT 
GACTGCTATG AAATTATAGA AAAAGACATC AAGGAAGCTG GAAATATAAC AACTGATGGT 
TATGAAATTC TTGGAAAACT CCTTAAAGGG GAAAGGAAAT CAGCCGCATT CCAAATCCAG 
AGTTTTGACA TTGTCTGCAG TCCAGTGTGG ACCAGTAGAG ACAGATGCTG TGATATTCCC 
TCTAGGAGAG ATGAGGGAAA ATGCCCTGCT TTTCCAAATT CTTGCACATG TACACAGGAC 
AGCGTTGGAC CTCCAGGACC TCCAGGCCCT GCAGGAGGAC CTGGTGCTAA AGGTCCCAGA 
GGTGAAAGAG GTATCAGTGG GGCAATTGGG CCCCCTGGTC CTCGTGGAGA CATAGGTCCT 
CCAGGCCCCC AGGGTCCTCC AGGCCCTCAG GGACCCAATG GACTCTCTAT TCCGGGAGAG 
CAAGGTCGCC AAGGGATGAA AGGTGATGCT GGAGAGCCAG GACTTCCAGG CCGAACAGGA 
ACCCCAGGAT TACCTGGCCC ACCAGGACCA ATGGGACCTC CAGGAGACAG AGGCTTCACT 
GGAAAAGACG GTGCAATGGG ACCCAGGGGC CCACCAGGGC CGCCGGGAAG CCCAGGCTCC 
CCAGGAGTCA CAGGACCAAG TGGGAAGCCA GGAAAACCTG GAGATCATGG CAGACCAGGT 
CCATCTGGGT TGAAAGGAGA AAAAGGTGAT AGGGGAGACA TTGCTTCCCA GAACATGATG 
CGAGCAGTTG CAAGACAAGT CTGTGAACAA TTGATAAGTG GTCAGATGAA CAGATTCAAT 
CAGATGCTGA ATCAGATTCC AAATGATTAC CAGTCCAGTC GCAACCAGCC AGGCCCGCCG 
GGTCCACCGG GACCTCCTGG TAGCGCAGGA GCCAGAGGAG AACCTGGGCC TGGGGGGCGG 
CCAGGCTTCC CGGGCACACC AGGGATGCAG GGACCCCCTG GGGAACGAGG TTTGCCAGGA 
GAGAAAGGTG AAAGGGGTAC TGGATCTTCA GGACCTCGGG GGCTGCCTGG CCCCCCAGGT 
CCACAAGGAG AATCCAGAAC AGGTCCACCA GGGTCCACAG GTTCAAGAGG TCCCCCTGGC 
CCCCCTGGCC GTCCTGGAAA CTCAGGTATC CGAGGACCCC CAGGTCCTCC TGGATACTGT 
GATTCTTCTC AGTGTGCCAG CATCCCATAC AACGGGCAAG GCTATCCAGA GCCTTACGTG 
CCCGAGGGCG GGGCCTACCT GCCTGAGCGC GAGCCCTTCA TCGTGCCGGT GGAGCCCGAG 
CGGACAGCGG AATACGAGGA CTACGGCGCA GACGAGCCTG CAGAGGAGCC TCCTGAGCCC 
CACCGGCGCT GGCGGCGCGC CCTGCCCCAC GGACCCGGGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011533738.1
CDS	522..6386
Translation	MRSRLPPALAALGAALLLSSIEAEGMECLTRAEADIVLLVDGSWSIGRANFRTVRSFISRIVEVFDIGPKRVQIALAQYSGDPRTEWQLNAHRDKKSLLQAVANLPYKGGNTLTGMALNFIRQQNFRTQAGMRPRARKIGVLITDGKSQDDVEAPSKKLKDEGVELFAIGIKNADEVELKMIATDPDDTHAYNVADFESLSRIVDDLTINLCNSVKGPGDLEAPSNLVISERTHRSFRVSWTPPSDSVDRYKVEYYPVSGGKRQEFYVSRMETSTVLKDLKPETEYVVNVYSVVEDEYSEPLKGTEKTLPVPVVSLNIYDVGPTTMHVQWQPVGGATGYILSYKPVKDTEPTRPKEVRLGPTVNDMQLTDLVPNTEYAVTVQAVLHDLTSEPVTVREVTLPLPRPQDLKLRDVTHSTMNVFWEPVPGKVRKYIVRYKTPEEDVKEVEVDRSETSTSLKDLFSQTLYTVSVSAVHDEGESPPVTAQETTRPVPAPTNLKITEVTSEGFRGTWDHGASDVSLYRITWAPFGSSDKMETILNGDENTLVFENLNPNTIYEVSITAIYPDESESDDLIGSERTLPILTTQAPKSGPRNLQVYNATSNSLTVKWDPASGRVQKYRITYQPSTGEGNEQTTTIGGRQNSVVLQKLKPDTPYTITVSSLYPDGEGGRMTGRGKTKPLNTVRNLRVYDPSTSTLNVRWDHAEGNPRQYKLFYAPAAGGPEELVPIPGNTNYAILRNLQPDTSYTVTVVPVYTEGDGGRTSDTGRTLMRGLARNVQVYNPTPNSLDVRWDPAPGPVLQYRVVYSPVDGTRPSESIVVPGNTRMVHLERLIPDTLYSVNLVALYSDGEGNPSPAQGRTLPRSGPRNLRVFGETTNSLSVAWDHADGPVQQYRIIYSPTVGDPIDEYTTVPGRRNNVILQPLQPDTPYKITVIAVYEDGDGGHLTGNGRTVGLLPPQNIHISDEWYTRFRVSWDPSPSPVLGYKIVYKPVGSNEPMEAFVGEMTSYTLHNLNPSTTYDVNVYAQYDSGLSVPLTDQGTTLYLNVTDLKTYQIGWDTFCVKWSPHRAATSYRLKLSPADGTRGQEITVRGSETSHCFTGLSPDTDYGVTVFVQTPNLEGPGVSVKEHTTVKPTEAPTEPPTPPPPPTIPPARDVCKGAKADIVFLTDASWSIGDDNFNKVVKFIFNTVGGFDEISPAGIQVSFVQYSDEVKSEFKLNTYNDKALALGALQNIRYRGGNTRTGKALTFIKEKVLTWESGMRKNVPKVLVVVTDGRSQDEVKKAALVIQQSGFSVFVVGVADVDYNELANIASKPSERHVFIVDDFESFEKIEDNLITFVCETATSSCPLIYLDGYTSPGFKMLEAYNLTEKNFASVQGVSLESGSFPSYSAYRIQKNAFVNQPTADLHPNGLPPSYTIILLFRLLPETPSDPFAIWQITDRDYKPQVGVIADPSSKTLSFFNKDTRGEVQTVTFDTEEVKTLFYGSFHKVHIVVTSKSVKIYIDCYEIIEKDIKEAGNITTDGYEILGKLLKGERKSAAFQIQSFDIVCSPVWTSRDRCCDIPSRRDEGKCPAFPNSCTCTQDSVGPPGPPGPAGGPGAKGPRGERGISGAIGPPGPRGDIGPPGPQGPPGPQGPNGLSIPGEQGRQGMKGDAGEPGLPGRTGTPGLPGPPGPMGPPGDRGFTGKDGAMGPRGPPGPPGSPGSPGVTGPSGKPGKPGDHGRPGPSGLKGEKGDRGDIASQNMMRAVARQVCEQLISGQMNRFNQMLNQIPNDYQSSRNQPGPPGPPGPPGSAGARGEPGPGGRPGFPGTPGMQGPPGERGLPGEKGERGTGSSGPRGLPGPPGPQGESRTGPPGSTGSRGPPGPPGRPGNSGIRGPPGPPGYCDSSQCASIPYNGQGYPEPYVPEGGAYLPEREPFIVPVEPERTAEYEDYGADEPAEEPPEPHRRWRRALPHGPGQ

Target ORF information:

RefSeq Version	XM_011535436.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XII alpha 1 chain (COL12A1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535436.2

ORF Insert Sequence:

ATGCGGAGTA GGCTTCCCCC AGCGCTTGCC GCCCTGGGCG CGGCCCTGCT CCTGTCTTCC 
ATTGAGGCAG AAGGGATGGA GTGTCTCACC AGAGCTGAGG CAGACATTGT GTTGCTGGTG 
GATGGATCAT GGAGCATCGG CCGGGCAAAT TTTAGAACCG TGAGGAGTTT CATTTCTCGT 
ATTGTGGAAG TCTTTGACAT TGGCCCCAAA AGAGTACAAA TTGCTCTTGC TCAGTATAGT 
GGGGATCCCA GAACAGAGTG GCAGTTAAAT GCACACAGAG ACAAGAAGAG CTTGTTGCAA 
GCTGTGGCAA ACTTGCCGTA CAAAGGAGGC AATACTCTCA CAGGCATGGC TTTGAATTTC 
ATTCGCCAAC AGAACTTCAG GACCCAAGCT GGCATGAGAC CTCGAGCTCG AAAAATTGGT 
GTGCTCATTA CTGATGGAAA ATCACAAGAC GATGTTGAAG CACCTTCAAA GAAACTCAAG 
GATGAGGGAG TGGAGCTGTT TGCTATTGGT ATTAAAAATG CTGATGAAGT CGAATTAAAG 
ATGATTGCAA CTGATCCTGA TGATACCCAT GCATACAATG TGGCAGATTT TGAGTCACTC 
TCCAGGATAG TGGATGATCT CACCATTAAT TTGTGTAACA GTGTCAAAGG TCCAGGTGAT 
TTGGAAGCAC CTTCTAACTT AGTTATTTCT GAGCGAACCC ATCGTTCTTT TAGAGTGAGC 
TGGACACCAC CTTCTGACAG TGTGGATCGA TATAAGGTGG AATACTATCC AGTTTCTGGA 
GGGAAACGTC AAGAATTTTA TGTGAGTCGA ATGGAAACTA GCACAGTGCT GAAAGATCTG 
AAACCTGAAA CTGAATATGT TGTCAATGTG TATTCTGTGG TAGAAGATGA ATATAGTGAG 
CCTCTGAAGG GGACAGAAAA AACCTTGCCA GTGCCTGTAG TCAGCCTGAA TATTTATGAT 
GTTGGCCCTA CCACCATGCA TGTGCAGTGG CAGCCTGTGG GAGGAGCTAC TGGCTACATC 
TTGTCATACA AACCTGTTAA GGACACAGAG CCAACAAGAC CCAAAGAGGT GCGTTTGGGG 
CCAACAGTGA ATGACATGCA GCTGACTGAC CTTGTTCCCA ACACGGAGTA TGCAGTCACA 
GTCCAGGCTG TCCTGCACGA CCTCACTAGT GAACCTGTCA CTGTTCGGGA AGTCACCTTG 
CCTTTACCCA GACCTCAGGA TCTGAAACTC AGAGATGTGA CTCACAGCAC TATGAATGTC 
TTTTGGGAAC CTGTGCCTGG AAAAGTGCGT AAATATATTG TTCGATACAA AACACCAGAA 
GAGGATGTCA AAGAGGTAGA GGTGGACAGA TCAGAGACCA GCACTTCCCT CAAAGACCTC 
TTCTCACAGA CCTTGTACAC AGTCAGCGTT TCTGCAGTAC ATGACGAGGG GGAGTCTCCT 
CCAGTGACTG CTCAAGAAAC TACCCGACCC GTGCCAGCCC CAACAAACTT AAAGATTACT 
GAAGTAACAT CAGAGGGTTT CAGAGGGACT TGGGATCATG GAGCTTCAGA TGTGTCTCTC 
TACAGAATAA CTTGGGCACC TTTTGGAAGC TCAGATAAGA TGGAGACCAT CTTAAATGGA 
GATGAAAACA CTTTGGTGTT CGAAAACCTG AACCCCAACA CCATCTATGA AGTTTCCATT 
ACTGCCATCT ATCCTGATGA GTCAGAAAGT GATGACCTGA TTGGCAGTGA GCGCACACTG 
CCTATCTTAA CAACACAAGC TCCCAAAAGT GGCCCACGAA ACCTTCAAGT GTACAATGCA 
ACATCTAACA GCCTGACTGT TAAGTGGGAT CCTGCTAGTG GTCGTGTGCA GAAATATAGG 
ATCACTTATC AGCCTTCCAC AGGGGAAGGC AATGAGCAAA CGACCACAAT AGGAGGACGG 
CAGAACAGTG TGGTCCTGCA GAAACTGAAG CCAGACACTC CTTACACTAT CACCGTATCC 
TCTCTGTATC CTGATGGTGA AGGAGGTCGG ATGACGGGAA GAGGCAAGAC CAAACCTCTA 
AACACTGTAA GGAACCTGAG AGTGTATGAC CCTTCTACCA GCACCTTGAA TGTCCGCTGG 
GACCATGCAG AGGGAAATCC TCGTCAGTAC AAGCTCTTCT ATGCACCAGC AGCAGGTGGT 
CCAGAGGAAC TGGTACCAAT CCCCGGGAAT ACCAATTATG CCATTCTTAG GAATCTGCAG 
CCAGATACCT CATACACTGT GACTGTAGTT CCCGTTTATA CTGAAGGTGA TGGGGGACGC 
ACATCAGATA CTGGAAGGAC ATTGATGAGA GGACTGGCAA GAAATGTCCA AGTATACAAT 
CCTACACCTA ACAGCCTCGA TGTTCGCTGG GACCCTGCTC CAGGACCTGT GCTGCAATAT 
CGCGTTGTGT ATTCTCCTGT GGATGGCACA AGACCCTCAG AATCTATAGT AGTGCCAGGA 
AACACGCGCA TGGTGCATCT GGAGCGGCTG ATTCCGGACA CACTCTATTC CGTGAACCTT 
GTGGCTCTGT ACTCGGATGG AGAGGGAAAT CCCAGCCCTG CCCAGGGCCG AACGCTACCA 
CGCAGTGGAC CAAGGAACCT GAGAGTCTTT GGTGAAACAA CCAATAGCCT CTCGGTAGCC 
TGGGATCATG CTGATGGGCC AGTTCAGCAG TACAGGATCA TCTATTCTCC CACTGTTGGT 
GATCCAATTG ATGAATATAC CACAGTCCCA GGCAGAAGAA ACAATGTAAT ACTGCAGCCC 
CTGCAACCTG ACACTCCATA TAAAATTACT GTTATTGCTG TTTATGAAGA TGGAGATGGT 
GGCCATCTAA CAGGAAATGG AAGAACTGTG GGACTCCTTC CTCCTCAGAA CATACACATC 
TCTGACGAAT GGTATACAAG ATTCAGGGTG TCCTGGGATC CTTCACCTTC TCCAGTTCTT 
GGATATAAAA TAGTATATAA GCCAGTGGGT TCCAATGAGC CCATGGAAGC CTTTGTTGGA 
GAAATGACAT CATATACCTT ACACAATCTC AATCCCAGCA CCACCTACGA TGTGAATGTT 
TATGCTCAAT ATGATTCTGG ACTCAGTGTC CCCTTGACAG ATCAAGGCAC TACATTATAT 
TTAAATGTAA CAGATCTGAA AACTTACCAG ATTGGGTGGG ATACATTCTG TGTCAAATGG 
TCACCTCACC GGGCAGCCAC CTCCTACAGG CTAAAACTAA GCCCTGCAGA TGGAACAAGG 
GGACAAGAAA TTACAGTGCG TGGATCAGAA ACCAGTCACT GCTTCACTGG CCTTTCACCA 
GACACTGATT ATGGTGTCAC TGTTTTTGTG CAGACACCAA ATCTCGAGGG ACCAGGAGTC 
TCTGTTAAAG AACATACCAC TGTGAAACCA ACAGAAGCCC CTACAGAGCC ACCCACACCT 
CCTCCCCCTC CCACCATTCC ACCAGCCCGG GATGTATGCA AAGGGGCCAA GGCAGATATT 
GTGTTCTTGA CTGATGCCTC CTGGAGCATT GGGGACGATA ATTTTAACAA AGTTGTAAAA 
TTCATCTTCA ATACTGTGGG AGGCTTTGAT GAAATCAGTC CTGCTGGGAT TCAGGTTTCA 
TTTGTGCAAT ACAGCGATGA GGTCAAGTCT GAGTTCAAGC TGAACACGTA CAATGACAAG 
GCCCTAGCCC TTGGGGCCCT CCAGAATATT AGGTACAGAG GAGGAAACAC AAGAACAGGC 
AAGGCCCTCA CGTTTATCAA GGAGAAAGTC TTGACTTGGG AGAGCGGCAT GAGGAAGAAT 
GTCCCTAAGG TGTTGGTTGT GGTCACGGAC GGTCGGTCCC AGGATGAGGT CAAGAAGGCG 
GCTTTGGTCA TCCAGCAGTC AGGGTTCAGT GTCTTTGTAG TTGGTGTGGC TGATGTCGAC 
TACAATGAGC TTGCCAACAT TGCCAGCAAA CCAAGTGAAC GGCACGTGTT CATTGTGGAC 
GACTTTGAAT CTTTTGAGAA GATCGAAGAC AATCTTATTA CATTTGTTTG TGAAACTGCC 
ACTTCAAGTT GTCCTCTCAT TTATTTGGAT GGCTACACCT CACCAGGTTT TAAAATGCTT 
GAAGCATACA ACCTGACAGA AAAGAATTTT GCTTCTGTAC AAGGAGTATC TTTGGAGTCA 
GGGTCTTTCC CCAGCTACTC AGCATACAGG ATTCAGAAGA ATGCGTTTGT GAATCAGCCT 
ACAGCAGACC TACACCCAAA TGGACTCCCT CCTTCATACA CGATTATATT ATTATTCAGA 
CTTCTCCCAG AAACTCCCAG TGACCCTTTT GCAATTTGGC AAATCACAGA CAGAGACTAC 
AAACCACAAG TTGGAGTGAT TGCAGATCCT TCTAGCAAGA CGTTATCATT CTTTAACAAG 
GATACAAGAG GCGAGGTGCA AACTGTTACA TTTGACACAG AAGAAGTAAA GACATTATTT 
TATGGAAGTT TTCACAAGGT TCATATTGTA GTGACCTCAA AAAGTGTTAA GATTTACATT 
GACTGCTATG AAATTATAGA AAAAGACATC AAGGAAGCTG GAAATATAAC AACTGATGGT 
TATGAAATTC TTGGAAAACT CCTTAAAGGG GAAAGGAAAT CAGCCGCATT CCAAATCCAG 
AGTTTTGACA TTGTCTGCAG TCCAGTGTGG ACCAGTAGAG ACAGATGCTG TGATATTCCC 
TCTAGGAGAG ATGAGGGAAA ATGCCCTGCT TTTCCAAATT CTTGCACATG TACACAGGAC 
AGCGTTGGAC CTCCAGGACC TCCAGGCCCT GCAGGAGGAC CTGGTGCTAA AGGTCCCAGA 
GGTGAAAGAG GTATCAGTGG GGCAATTGGG CCCCCTGGTC CTCGTGGAGA CATAGGTCCT 
CCAGGCCCCC AGGGTCCTCC AGGCCCTCAG GGACCCAATG GACTCTCTAT TCCGGGAGAG 
CAAGGTCGCC AAGGGATGAA AGGTGATGCT GGAGAGCCAG GACTTCCAGG CCGAACAGGA 
ACCCCAGGAT TACCTGGCCC ACCAGGACCA ATGGGACCTC CAGGAGACAG AGGCTTCACT 
GGAAAAGACG GTGCAATGGG ACCCAGGGGC CCACCAGGGC CGCCGGGAAG CCCAGGCTCC 
CCAGGAGTCA CAGGACCAAG TGGGAAGCCA GGAAAACCTG GAGATCATGG CAGACCAGGT 
CCATCTGGGT TGAAAGGAGA AAAAGGTGAT AGGGGAGACA TTGCTTCCCA GAACATGATG 
CGAGCAGTTG CAAGACAAGT CTGTGAACAA TTGATAAGTG GTCAGATGAA CAGATTCAAT 
CAGATGCTGA ATCAGATTCC AAATGATTAC CAGTCCAGTC GCAACCAGCC AGGCCCGCCG 
GGTCCACCGG GACCTCCTGG TAGCGCAGGA GCCAGAGGAG AACCTGGGCC TGGGGGGCGG 
CCAGGCTTCC CGGGCACACC AGGGATGCAG GGACCCCCTG GGGAACGAGG TTTGCCAGGA 
GAGAAAGGTG AAAGGGGTAC TGGATCTTCA GGACCTCGGG GGCTGCCTGG CCCCCCAGGT 
CCACAAGGAG AATCCAGAAC AGGTCCACCA GGGTCCACAG GTTCAAGAGG TCCCCCTGGC 
CCCCCTGGCC GTCCTGGAAA CTCAGGTATC CGAGGACCCC CAGGTCCTCC TGGATACTGT 
GATTCTTCTC AGTGTGCCAG CATCCCATAC AACGGGCAAG GCTATCCAGA GCCTTACGTG 
CCCGAGGGCG GGGCCTACCT GCCTGAGCGC GAGCCCTTCA TCGTGCCGGT GGAGCCCGAG 
CGGACAGCGG AATACGAGGA CTACGGCGCA GACGAGCCTG CAGAGGAGCC TCCTGAGCCC 
CACCGGCGCT GGCGGCGCGC CCTGCCCCAC GGACCCGGGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL12A1 ( XM_017010252.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865741.1 Homo sapiens collagen type XII alpha 1 chain (COL12A1), transcript variant X2, mRNA.

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CloneID	OHu96293
Clone ID Related Accession (Same CDS sequence)	XM_017010252.2 , XM_017010252.1
Accession Version	XM_017010252.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(XII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017010252.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAACA GACAAGACCC CAGTCTTCTA GTCCTAGTTG ACCCACCTTC AGACTTGAAT 
TTTAAAATTA TAGATGAAAA TACTGTTCAT ATGTCATGGG CAAAACCAGT TGATCCAATT 
GTGGGTTACA GAATAACGGT GGACCCTACA ACGGATGGGC CTACTAAAGA ATTTACCCTT 
TCAGCTAGTA CCACTGAAAC TTTATTGTCA GAACTTGTAC CTGAAACAGA GTATGTGGTG 
ACAATAACTT CATATGATGA AGTAGAAGAA AGTGTACCAG TTATAGGACA ACTAACAATT 
CAAACAGGTA GTTCGACAAA GCCAGTGGAG AAGAAACCTG GAAAAACCGA GATACAAAAA 
TGCTCTGTCA GTGCCTGGAC TGATTTGGTT TTCCTCGTGG ATGGCTCTTG GAGTGTGGGA 
AGAAATAATT TCAAGTACAT TTTAGACTTC ATTGCTGCTC TTGTGTCTGC TTTTGACATT 
GGGGAAGAGA AGACAAGAGT TGGAGTTGTT CAATACAGCT CTGATACCAG GACTGAATTT 
AACTTAAATC AGTACTACCA AAGGGATGAA CTTCTTGCTG CAATAAAAAA AATTCCATAT 
AAAGGTGGCA ACACAATGAC AGGGGATGCC ATTGATTATT TAGTTAAAAA TACTTTCACG 
GAATCTGCTG GGGCAAGAGT TGGCTTTCCT AAAGTGGCAA TTATTATTAC GGATGGAAAA 
TCCCAGGATG AAGTGGAAAT TCCAGCAAGA GAGCTTCGTA ATGTTGGAGT TGAAGTTTTC 
TCCTTGGGCA TTAAAGCTGC AGATGCAAAA GAACTCAAAC AAATTGCCTC CACACCTTCA 
CTGAACCATG TTTTCAATGT GGCCAACTTT GATGCAATTG TGGATATTCA GAATGAGATC 
ATCTCCCAGG TGTGCTCAGG TGTTGATGAA CAACTTGGTG AATTGGTTAG TGGAGAAGAA 
GTTGTTGAGC CTCCTTCAAA TTTGATTGCC ATGGAAGTCT CTTCAAAATA TGTTAAGCTA 
AATTGGAATC CATCTCCTAG TCCAGTGACT GGCTACAAAG TCATCCTCAC ACCAATGACT 
GCAGGAAGCC GACAGCACGC TCTGAGTGTG GGGCCTCAGA CAACCACGCT CAGTGTTCGC 
GACCTCTCAG CAGACACAGA ATACCAGATC AGTGTTTCCG CCATGAAGGG AATGACATCC 
AGTGAACCCA TTTCAATAAT GGAGAAGACT CAGCCAATGA AAGTTCAAGT GGAATGCTCA 
CGTGGTGTGG ATATAAAAGC CGATATTGTG TTTTTGGTTG ATGGCTCCTA TAGCATTGGG 
ATTGCAAACT TTGTTAAAGT TAGAGCCTTT TTGGAAGTTC TTGTAAAAAG TTTTGAAATT 
TCACCAAATA GGGTCCAGAT TAGTCTTGTG CAATACAGCC GGGATCCTCA TACTGAGTTC 
ACTTTGAAAA AATTCACCAA AGTTGAAGAT ATAATTGAAG CAATAAACAC CTTCCCTTAC 
AGAGGAGGAT CTACAAATAC TGGCAAAGCA ATGACTTATG TCAGAGAGAA AATATTTGTG 
CCTAGCAAGG GATCAAGAAG CAATGTGCCA AAGGTCATGA TTCTTATCAC GGATGGGAAA 
TCATCAGATG CTTTCAGAGA TCCTGCGATA AAACTGAGGA ATTCAGATGT TGAAATCTTT 
GCAGTTGGTG TGAAGGATGC CGTTCGCTCA GAATTGGAAG CTATTGCCTC TCCTCCTGCA 
GAGACCCATG TGTTCACAGT GGAAGATTTT GATGCTTTTC AGAGGATATC TTTTGAACTC 
ACACAGTCTA TCTGCCTTAG AATTGAGCAA GAATTGGCAG CTATAAAGAA GAAAGCTTAC 
GTCCCTCCAA AGGATCTTAG TTTTTCAGAA GTGACTTCTT ATGGTTTCAA AACCAACTGG 
TCTCCAGCTG GAGAAAATGT TTTTTCATAT CACATCACCT ACAAGGAAGC GGCTGGGGAT 
GATGAGGTCA CTGTGGTGGA GCCAGCATCG AGCACCAGTG TTGTTCTCAG CAGCCTGAAG 
CCAGAGACCT TGTATTTGGT CAATGTGACT GCGGAGTATG AGGATGGCTT CAGCATTCCC 
TTAGCTGGAG AGGAGACCAC CGAAGAAGTA AAAGGAGCAC CTCGAAACCT AAAGGTGACA 
GATGAGACTA CAGATAGTTT CAAAATTACT TGGACTCAAG CTCCAGGGAG AGTTTTAAGA 
TATCGAATTA TATATAGACC AGTTGCTGGT GGAGAGAGCA GAGAAGTTAC CACCCCACCC 
AATCAGAGGA GGAGAACACT GGAGAACTTG ATTCCAGACA CGAAATATGA AGTATCTGTA 
ATTCCTGAAT ATTTCTCAGG ACCTGGTACT CCATTAACTG GAAATGCAGC CACTGAAGAA 
GTTAGAGGGA ATCCAAGAGA CTTAAGAGTT TCTGACCCTA CGACGTCTAC TATGAAATTA 
TCTTGGAGTG GGGCACCAGG AAAAGTGAAA CAGTATCTCG TCACATATAC CCCAGTGGCA 
GGGGGTGAAA CTCAAGAGGT CACTGTGAGG GGAGATACAA CCAATACGGT GCTGCAGGGA 
TTGAAGGAAG GGACACAATA CGCCTTATCT GTGACAGCCT TGTATGCGTC TGGGGCTGGA 
GACGCCCTCT TTGGTGAAGG AACAACACTT GAAGAACGTG GTTCTCCTCA AGATTTAGTT 
ACTAAAGACA TCACTGACAC ATCAATTGGG GCTTATTGGA CATCTGCTCC AGGAATGGTT 
CGCGGTTACA GGGTCTCATG GAAATCACTT TATGATGATG TTGACACTGG AGAGAAAAAT 
CTGCCTGAAG ATGCAATTCA TACGATGATA GAAAATCTGC AGCCAGAGAC CAAATACAGA 
ATTTCAGTAT TTGCCACTTA CAGCAGTGGA GAAGGAGAAC CTTTGACTGG AGATGCCACA 
ACTGAATTAT CTCAAGATTC CAAAACCCTG AAAGTAGATG AAGAAACAGA AAACACAATG 
AGAGTTACAT GGAAACCAGC ACCAGGGAAA GTCGTCAACT ACCGTGTTGT CTATCGCCCT 
CATGGGAGAG GGAAGCAAAT GGTTGCTAAG GTGCCCCCCA CAGTCACTTC GACAGTGTTA 
AAGCGACTTC AGCCACAGAC CACATATGAC ATCACAGTTC TTCCTATTTA CAAGATGGGA 
GAAGGAAAGC TTAGGCAAGG ATCAGGAACA ACAGCTTCTC GGTTTAAGTC TCCTAGAAAC 
CTCAAAACAT CTGACCCAAC CATGTCAAGC TTCCGAGTGA CTTGGGAGCC TGCCCCTGGG 
GAAGTGAAGG GTTATAAAGT CACATTCCAC CCTACGGGGG ATGACAGAAG ACTGGGGGAG 
TTAGTGGTTG GACCCTATGA CAACACAGTT GTTTTGGAGG AACTTAGGGC TGGTACCACC 
TATAAAGTAA ATGTTTTTGG AATGTTTGAT GGAGGAGAAA GCTCACCACT TGTTGGACAA 
GAAATGACAA CCCTTTCCGA CACAACTGTT ATGCCAATTT TATCTTCTGG GATGGAGTGT 
CTCACCAGAG CTGAGGCAGA CATTGTGTTG CTGGTGGATG GATCATGGAG CATCGGCCGG 
GCAAATTTTA GAACCGTGAG GAGTTTCATT TCTCGTATTG TGGAAGTCTT TGACATTGGC 
CCCAAAAGAG TACAAATTGC TCTTGCTCAG TATAGTGGGG ATCCCAGAAC AGAGTGGCAG 
TTAAATGCAC ACAGAGACAA GAAGAGCTTG TTGCAAGCTG TGGCAAACTT GCCGTACAAA 
GGAGGCAATA CTCTCACAGG CATGGCTTTG AATTTCATTC GCCAACAGAA CTTCAGGACC 
CAAGCTGGCA TGAGACCTCG AGCTCGAAAA ATTGGTGTGC TCATTACTGA TGGAAAATCA 
CAAGACGATG TTGAAGCACC TTCAAAGAAA CTCAAGGATG AGGGAGTGGA GCTGTTTGCT 
ATTGGTATTA AAAATGCTGA TGAAGTCGAA TTAAAGATGA TTGCAACTGA TCCTGATGAT 
ACCCATGCAT ACAATGTGGC AGATTTTGAG TCACTCTCCA GGATAGTGGA TGATCTCACC 
ATTAATTTGT GTAACAGTGT CAAAGGTCCA GGTGATTTGG AAGCACCTTC TAACTTAGTT 
ATTTCTGAGC GAACCCATCG TTCTTTTAGA GTGAGCTGGA CACCACCTTC TGACAGTGTG 
GATCGATATA AGGTGGAATA CTATCCAGTT TCTGGAGGGA AACGTCAAGA ATTTTATGTG 
AGTCGAATGG AAACTAGCAC AGTGCTGAAA GATCTGAAAC CTGAAACTGA ATATGTTGTC 
AATGTGTATT CTGTGGTAGA AGATGAATAT AGTGAGCCTC TGAAGGGGAC AGAAAAAACC 
TTGCCAGTGC CTGTAGTCAG CCTGAATATT TATGATGTTG GCCCTACCAC CATGCATGTG 
CAGTGGCAGC CTGTGGGAGG AGCTACTGGC TACATCTTGT CATACAAACC TGTTAAGGAC 
ACAGAGCCAA CAAGACCCAA AGAGGTGCGT TTGGGGCCAA CAGTGAATGA CATGCAGCTG 
ACTGACCTTG TTCCCAACAC GGAGTATGCA GTCACAGTCC AGGCTGTCCT GCACGACCTC 
ACTAGTGAAC CTGTCACTGT TCGGGAAGTC ACCTTGCCTT TACCCAGACC TCAGGATCTG 
AAACTCAGAG ATGTGACTCA CAGCACTATG AATGTCTTTT GGGAACCTGT GCCTGGAAAA 
GTGCGTAAAT ATATTGTTCG ATACAAAACA CCAGAAGAGG ATGTCAAAGA GGTAGAGGTG 
GACAGATCAG AGACCAGCAC TTCCCTCAAA GACCTCTTCT CACAGACCTT GTACACAGTC 
AGCGTTTCTG CAGTACATGA CGAGGGGGAG TCTCCTCCAG TGACTGCTCA AGAAACTACC 
CGACCCGTGC CAGCCCCAAC AAACTTAAAG ATTACTGAAG TAACATCAGA GGGTTTCAGA 
GGGACTTGGG ATCATGGAGC TTCAGATGTG TCTCTCTACA GAATAACTTG GGCACCTTTT 
GGAAGCTCAG ATAAGATGGA GACCATCTTA AATGGAGATG AAAACACTTT GGTGTTCGAA 
AACCTGAACC CCAACACCAT CTATGAAGTT TCCATTACTG CCATCTATCC TGATGAGTCA 
GAAAGTGATG ACCTGATTGG CAGTGAGCGC ACACTGCCTA TCTTAACAAC ACAAGCTCCC 
AAAAGTGGCC CACGAAACCT TCAAGTGTAC AATGCAACAT CTAACAGCCT GACTGTTAAG 
TGGGATCCTG CTAGTGGTCG TGTGCAGAAA TATAGGATCA CTTATCAGCC TTCCACAGGG 
GAAGGCAATG AGCAAACGAC CACAATAGGA GGACGGCAGA ACAGTGTGGT CCTGCAGAAA 
CTGAAGCCAG ACACTCCTTA CACTATCACC GTATCCTCTC TGTATCCTGA TGGTGAAGGA 
GGTCGGATGA CGGGAAGAGG CAAGACCAAA CCTCTAAACA CTGTAAGGAA CCTGAGAGTG 
TATGACCCTT CTACCAGCAC CTTGAATGTC CGCTGGGACC ATGCAGAGGG AAATCCTCGT 
CAGTACAAGC TCTTCTATGC ACCAGCAGCA GGTGGTCCAG AGGAACTGGT ACCAATCCCC 
GGGAATACCA ATTATGCCAT TCTTAGGAAT CTGCAGCCAG ATACCTCATA CACTGTGACT 
GTAGTTCCCG TTTATACTGA AGGTGATGGG GGACGCACAT CAGATACTGG AAGGACATTG 
ATGAGAGGAC TGGCAAGAAA TGTCCAAGTA TACAATCCTA CACCTAACAG CCTCGATGTT 
CGCTGGGACC CTGCTCCAGG ACCTGTGCTG CAATATCGCG TTGTGTATTC TCCTGTGGAT 
GGCACAAGAC CCTCAGAATC TATAGTAGTG CCAGGAAACA CGCGCATGGT GCATCTGGAG 
CGGCTGATTC CGGACACACT CTATTCCGTG AACCTTGTGG CTCTGTACTC GGATGGAGAG 
GGAAATCCCA GCCCTGCCCA GGGCCGAACG CTACCACGCA GTGGACCAAG GAACCTGAGA 
GTCTTTGGTG AAACAACCAA TAGCCTCTCG GTAGCCTGGG ATCATGCTGA TGGGCCAGTT 
CAGCAGTACA GGATCATCTA TTCTCCCACT GTTGGTGATC CAATTGATGA ATATACCACA 
GTCCCAGGCA GAAGAAACAA TGTAATACTG CAGCCCCTGC AACCTGACAC TCCATATAAA 
ATTACTGTTA TTGCTGTTTA TGAAGATGGA GATGGTGGCC ATCTAACAGG AAATGGAAGA 
ACTGTGGGAC TCCTTCCTCC TCAGAACATA CACATCTCTG ACGAATGGTA TACAAGATTC 
AGGGTGTCCT GGGATCCTTC ACCTTCTCCA GTTCTTGGAT ATAAAATAGT ATATAAGCCA 
GTGGGTTCCA ATGAGCCCAT GGAAGCCTTT GTTGGAGAAA TGACATCATA TACCTTACAC 
AATCTCAATC CCAGCACCAC CTACGATGTG AATGTTTATG CTCAATATGA TTCTGGACTC 
AGTGTCCCCT TGACAGATCA AGGCACTACA TTATATTTAA ATGTAACAGA TCTGAAAACT 
TACCAGATTG GGTGGGATAC ATTCTGTGTC AAATGGTCAC CTCACCGGGC AGCCACCTCC 
TACAGGCTAA AACTAAGCCC TGCAGATGGA ACAAGGGGAC AAGAAATTAC AGTGCGTGGA 
TCAGAAACCA GTCACTGCTT CACTGGCCTT TCACCAGACA CTGATTATGG TGTCACTGTT 
TTTGTGCAGA CACCAAATCT CGAGGGACCA GGAGTCTCTG TTAAAGAACA TACCACTGTG 
AAACCAACAG AAGCCCCTAC AGAGCCACCC ACACCTCCTC CCCCTCCCAC CATTCCACCA 
GCCCGGGATG TATGCAAAGG GGCCAAGGCA GATATTGTGT TCTTGACTGA TGCCTCCTGG 
AGCATTGGGG ACGATAATTT TAACAAAGTT GTAAAATTCA TCTTCAATAC TGTGGGAGGC 
TTTGATGAAA TCAGTCCTGC TGGGATTCAG GTTTCATTTG TGCAATACAG CGATGAGGTC 
AAGTCTGAGT TCAAGCTGAA CACGTACAAT GACAAGGCCC TAGCCCTTGG GGCCCTCCAG 
AATATTAGGT ACAGAGGAGG AAACACAAGA ACAGGCAAGG CCCTCACGTT TATCAAGGAG 
AAAGTCTTGA CTTGGGAGAG CGGCATGAGG AAGAATGTCC CTAAGGTGTT GGTTGTGGTC 
ACGGACGGTC GGTCCCAGGA TGAGGTCAAG AAGGCGGCTT TGGTCATCCA GCAGTCAGGG 
TTCAGTGTCT TTGTAGTTGG TGTGGCTGAT GTCGACTACA ATGAGCTTGC CAACATTGCC 
AGCAAACCAA GTGAACGGCA CGTGTTCATT GTGGACGACT TTGAATCTTT TGAGAAGATC 
GAAGACAATC TTATTACATT TGTTTGTGAA ACTGCCACTT CAAGTTGTCC TCTCATTTAT 
TTGGATGGCT ACACCTCACC AGGTTTTAAA ATGCTTGAAG CATACAACCT GACAGAAAAG 
AATTTTGCTT CTGTACAAGG AGTATCTTTG GAGTCAGGGT CTTTCCCCAG CTACTCAGCA 
TACAGGATTC AGAAGAATGC GTTTGTGAAT CAGCCTACAG CAGACCTACA CCCAAATGGA 
CTCCCTCCTT CATACACGAT TATATTATTA TTCAGACTTC TCCCAGAAAC TCCCAGTGAC 
CCTTTTGCAA TTTGGCAAAT CACAGACAGA GACTACAAAC CACAAGTTGG AGTGATTGCA 
GATCCTTCTA GCAAGACGTT ATCATTCTTT AACAAGGATA CAAGAGGCGA GGTGCAAACT 
GTTACATTTG ACACAGAAGA AGTAAAGACA TTATTTTATG GAAGTTTTCA CAAGGTTCAT 
ATTGTAGTGA CCTCAAAAAG TGTTAAGATT TACATTGACT GCTATGAAAT TATAGAAAAA 
GACATCAAGG AAGCTGGAAA TATAACAACT GATGGTTATG AAATTCTTGG AAAACTCCTT 
AAAGGGGAAA GGAAATCAGC CGCATTCCAA ATCCAGAGTT TTGACATTGT CTGCAGTCCA 
GTGTGGACCA GTAGAGACAG ATGCTGTGAT ATTCCCTCTA GGAGAGATGA GGGAAAATGC 
CCTGCTTTTC CAAATTCTTG CACATGTACA CAGGACAGCG TTGGACCTCC AGGACCTCCA 
GGCCCTGCAG GAGGACCTGG TGCTAAAGGT CCCAGAGGTG AAAGAGGTAT CAGTGGGGCA 
ATTGGGCCCC CTGGTCCTCG TGGAGACATA GGTCCTCCAG GCCCCCAGGG TCCTCCAGGC 
CCTCAGGGAC CCAATGGACT CTCTATTCCG GGAGAGCAAG GTCGCCAAGG GATGAAAGGT 
GATGCTGGAG AGCCAGGACT TCCAGGCCGA ACAGGAACCC CAGGATTACC TGGCCCACCA 
GGACCAATGG GACCTCCAGG AGACAGAGGC TTCACTGGAA AAGACGGTGC AATGGGACCC 
AGGGGCCCAC CAGGGCCGCC GGGAAGCCCA GGCTCCCCAG GAGTCACAGG ACCAAGTGGG 
AAGCCAGGAA AACCTGGAGA TCATGGCAGA CCAGGTCCAT CTGGGTTGAA AGGAGAAAAA 
GGTGATAGGG GAGACATTGC TTCCCAGAAC ATGATGCGAG CAGTTGCAAG ACAAGTCTGT 
GAACAATTGA TAAGTGGTCA GATGAACAGA TTCAATCAGA TGCTGAATCA GATTCCAAAT 
GATTACCAGT CCAGTCGCAA CCAGCCAGGC CCGCCGGGTC CACCGGGACC TCCTGGTAGC 
GCAGGAGCCA GAGGAGAACC TGGGCCTGGG GGGCGGCCAG GCTTCCCGGG CACACCAGGG 
ATGCAGGGAC CCCCTGGGGA ACGAGGTTTG CCAGGAGAGA AAGGTGAAAG GGGTACTGGA 
TCTTCAGGAC CTCGGGGGCT GCCTGGCCCC CCAGGTCCAC AAGGAGAATC CAGAACAGGT 
CCACCAGGGT CCACAGGTTC AAGAGGTCCC CCTGGCCCCC CTGGCCGTCC TGGAAACTCA 
GGTATCCGAG GACCCCCAGG TCCTCCTGGA TACTGTGATT CTTCTCAGTG TGCCAGCATC 
CCATACAACG GGCAAGGCTA TCCAGAGCCT TACGTGCCCG AGGGCGGGGC CTACCTGCCT 
GAGCGCGAGC CCTTCATCGT GCCGGTGGAG CCCGAGCGGA CAGCGGAATA CGAGGACTAC 
GGCGCAGACG AGCCTGCAGA GGAGCCTCCT GAGCCCCACC GGCGCTGGCG GCGCGCCCTG 
CCCCACGGAC CCGGGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865741.1
CDS	1207..10527
Translation	MKNRQDPSLLVLVDPPSDLNFKIIDENTVHMSWAKPVDPIVGYRITVDPTTDGPTKEFTLSASTTETLLSELVPETEYVVTITSYDEVEESVPVIGQLTIQTGSSTKPVEKKPGKTEIQKCSVSAWTDLVFLVDGSWSVGRNNFKYILDFIAALVSAFDIGEEKTRVGVVQYSSDTRTEFNLNQYYQRDELLAAIKKIPYKGGNTMTGDAIDYLVKNTFTESAGARVGFPKVAIIITDGKSQDEVEIPARELRNVGVEVFSLGIKAADAKELKQIASTPSLNHVFNVANFDAIVDIQNEIISQVCSGVDEQLGELVSGEEVVEPPSNLIAMEVSSKYVKLNWNPSPSPVTGYKVILTPMTAGSRQHALSVGPQTTTLSVRDLSADTEYQISVSAMKGMTSSEPISIMEKTQPMKVQVECSRGVDIKADIVFLVDGSYSIGIANFVKVRAFLEVLVKSFEISPNRVQISLVQYSRDPHTEFTLKKFTKVEDIIEAINTFPYRGGSTNTGKAMTYVREKIFVPSKGSRSNVPKVMILITDGKSSDAFRDPAIKLRNSDVEIFAVGVKDAVRSELEAIASPPAETHVFTVEDFDAFQRISFELTQSICLRIEQELAAIKKKAYVPPKDLSFSEVTSYGFKTNWSPAGENVFSYHITYKEAAGDDEVTVVEPASSTSVVLSSLKPETLYLVNVTAEYEDGFSIPLAGEETTEEVKGAPRNLKVTDETTDSFKITWTQAPGRVLRYRIIYRPVAGGESREVTTPPNQRRRTLENLIPDTKYEVSVIPEYFSGPGTPLTGNAATEEVRGNPRDLRVSDPTTSTMKLSWSGAPGKVKQYLVTYTPVAGGETQEVTVRGDTTNTVLQGLKEGTQYALSVTALYASGAGDALFGEGTTLEERGSPQDLVTKDITDTSIGAYWTSAPGMVRGYRVSWKSLYDDVDTGEKNLPEDAIHTMIENLQPETKYRISVFATYSSGEGEPLTGDATTELSQDSKTLKVDEETENTMRVTWKPAPGKVVNYRVVYRPHGRGKQMVAKVPPTVTSTVLKRLQPQTTYDITVLPIYKMGEGKLRQGSGTTASRFKSPRNLKTSDPTMSSFRVTWEPAPGEVKGYKVTFHPTGDDRRLGELVVGPYDNTVVLEELRAGTTYKVNVFGMFDGGESSPLVGQEMTTLSDTTVMPILSSGMECLTRAEADIVLLVDGSWSIGRANFRTVRSFISRIVEVFDIGPKRVQIALAQYSGDPRTEWQLNAHRDKKSLLQAVANLPYKGGNTLTGMALNFIRQQNFRTQAGMRPRARKIGVLITDGKSQDDVEAPSKKLKDEGVELFAIGIKNADEVELKMIATDPDDTHAYNVADFESLSRIVDDLTINLCNSVKGPGDLEAPSNLVISERTHRSFRVSWTPPSDSVDRYKVEYYPVSGGKRQEFYVSRMETSTVLKDLKPETEYVVNVYSVVEDEYSEPLKGTEKTLPVPVVSLNIYDVGPTTMHVQWQPVGGATGYILSYKPVKDTEPTRPKEVRLGPTVNDMQLTDLVPNTEYAVTVQAVLHDLTSEPVTVREVTLPLPRPQDLKLRDVTHSTMNVFWEPVPGKVRKYIVRYKTPEEDVKEVEVDRSETSTSLKDLFSQTLYTVSVSAVHDEGESPPVTAQETTRPVPAPTNLKITEVTSEGFRGTWDHGASDVSLYRITWAPFGSSDKMETILNGDENTLVFENLNPNTIYEVSITAIYPDESESDDLIGSERTLPILTTQAPKSGPRNLQVYNATSNSLTVKWDPASGRVQKYRITYQPSTGEGNEQTTTIGGRQNSVVLQKLKPDTPYTITVSSLYPDGEGGRMTGRGKTKPLNTVRNLRVYDPSTSTLNVRWDHAEGNPRQYKLFYAPAAGGPEELVPIPGNTNYAILRNLQPDTSYTVTVVPVYTEGDGGRTSDTGRTLMRGLARNVQVYNPTPNSLDVRWDPAPGPVLQYRVVYSPVDGTRPSESIVVPGNTRMVHLERLIPDTLYSVNLVALYSDGEGNPSPAQGRTLPRSGPRNLRVFGETTNSLSVAWDHADGPVQQYRIIYSPTVGDPIDEYTTVPGRRNNVILQPLQPDTPYKITVIAVYEDGDGGHLTGNGRTVGLLPPQNIHISDEWYTRFRVSWDPSPSPVLGYKIVYKPVGSNEPMEAFVGEMTSYTLHNLNPSTTYDVNVYAQYDSGLSVPLTDQGTTLYLNVTDLKTYQIGWDTFCVKWSPHRAATSYRLKLSPADGTRGQEITVRGSETSHCFTGLSPDTDYGVTVFVQTPNLEGPGVSVKEHTTVKPTEAPTEPPTPPPPPTIPPARDVCKGAKADIVFLTDASWSIGDDNFNKVVKFIFNTVGGFDEISPAGIQVSFVQYSDEVKSEFKLNTYNDKALALGALQNIRYRGGNTRTGKALTFIKEKVLTWESGMRKNVPKVLVVVTDGRSQDEVKKAALVIQQSGFSVFVVGVADVDYNELANIASKPSERHVFIVDDFESFEKIEDNLITFVCETATSSCPLIYLDGYTSPGFKMLEAYNLTEKNFASVQGVSLESGSFPSYSAYRIQKNAFVNQPTADLHPNGLPPSYTIILLFRLLPETPSDPFAIWQITDRDYKPQVGVIADPSSKTLSFFNKDTRGEVQTVTFDTEEVKTLFYGSFHKVHIVVTSKSVKIYIDCYEIIEKDIKEAGNITTDGYEILGKLLKGERKSAAFQIQSFDIVCSPVWTSRDRCCDIPSRRDEGKCPAFPNSCTCTQDSVGPPGPPGPAGGPGAKGPRGERGISGAIGPPGPRGDIGPPGPQGPPGPQGPNGLSIPGEQGRQGMKGDAGEPGLPGRTGTPGLPGPPGPMGPPGDRGFTGKDGAMGPRGPPGPPGSPGSPGVTGPSGKPGKPGDHGRPGPSGLKGEKGDRGDIASQNMMRAVARQVCEQLISGQMNRFNQMLNQIPNDYQSSRNQPGPPGPPGPPGSAGARGEPGPGGRPGFPGTPGMQGPPGERGLPGEKGERGTGSSGPRGLPGPPGPQGESRTGPPGSTGSRGPPGPPGRPGNSGIRGPPGPPGYCDSSQCASIPYNGQGYPEPYVPEGGAYLPEREPFIVPVEPERTAEYEDYGADEPAEEPPEPHRRWRRALPHGPGQ

Target ORF information:

RefSeq Version	XM_017010252.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XII alpha 1 chain (COL12A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010252.2

ORF Insert Sequence:

ATGAAAAACA GACAAGACCC CAGTCTTCTA GTCCTAGTTG ACCCACCTTC AGACTTGAAT 
TTTAAAATTA TAGATGAAAA TACTGTTCAT ATGTCATGGG CAAAACCAGT TGATCCAATT 
GTGGGTTACA GAATAACGGT GGACCCTACA ACGGATGGGC CTACTAAAGA ATTTACCCTT 
TCAGCTAGTA CCACTGAAAC TTTATTGTCA GAACTTGTAC CTGAAACAGA GTATGTGGTG 
ACAATAACTT CATATGATGA AGTAGAAGAA AGTGTACCAG TTATAGGACA ACTAACAATT 
CAAACAGGTA GTTCGACAAA GCCAGTGGAG AAGAAACCTG GAAAAACCGA GATACAAAAA 
TGCTCTGTCA GTGCCTGGAC TGATTTGGTT TTCCTCGTGG ATGGCTCTTG GAGTGTGGGA 
AGAAATAATT TCAAGTACAT TTTAGACTTC ATTGCTGCTC TTGTGTCTGC TTTTGACATT 
GGGGAAGAGA AGACAAGAGT TGGAGTTGTT CAATACAGCT CTGATACCAG GACTGAATTT 
AACTTAAATC AGTACTACCA AAGGGATGAA CTTCTTGCTG CAATAAAAAA AATTCCATAT 
AAAGGTGGCA ACACAATGAC AGGGGATGCC ATTGATTATT TAGTTAAAAA TACTTTCACG 
GAATCTGCTG GGGCAAGAGT TGGCTTTCCT AAAGTGGCAA TTATTATTAC GGATGGAAAA 
TCCCAGGATG AAGTGGAAAT TCCAGCAAGA GAGCTTCGTA ATGTTGGAGT TGAAGTTTTC 
TCCTTGGGCA TTAAAGCTGC AGATGCAAAA GAACTCAAAC AAATTGCCTC CACACCTTCA 
CTGAACCATG TTTTCAATGT GGCCAACTTT GATGCAATTG TGGATATTCA GAATGAGATC 
ATCTCCCAGG TGTGCTCAGG TGTTGATGAA CAACTTGGTG AATTGGTTAG TGGAGAAGAA 
GTTGTTGAGC CTCCTTCAAA TTTGATTGCC ATGGAAGTCT CTTCAAAATA TGTTAAGCTA 
AATTGGAATC CATCTCCTAG TCCAGTGACT GGCTACAAAG TCATCCTCAC ACCAATGACT 
GCAGGAAGCC GACAGCACGC TCTGAGTGTG GGGCCTCAGA CAACCACGCT CAGTGTTCGC 
GACCTCTCAG CAGACACAGA ATACCAGATC AGTGTTTCCG CCATGAAGGG AATGACATCC 
AGTGAACCCA TTTCAATAAT GGAGAAGACT CAGCCAATGA AAGTTCAAGT GGAATGCTCA 
CGTGGTGTGG ATATAAAAGC CGATATTGTG TTTTTGGTTG ATGGCTCCTA TAGCATTGGG 
ATTGCAAACT TTGTTAAAGT TAGAGCCTTT TTGGAAGTTC TTGTAAAAAG TTTTGAAATT 
TCACCAAATA GGGTCCAGAT TAGTCTTGTG CAATACAGCC GGGATCCTCA TACTGAGTTC 
ACTTTGAAAA AATTCACCAA AGTTGAAGAT ATAATTGAAG CAATAAACAC CTTCCCTTAC 
AGAGGAGGAT CTACAAATAC TGGCAAAGCA ATGACTTATG TCAGAGAGAA AATATTTGTG 
CCTAGCAAGG GATCAAGAAG CAATGTGCCA AAGGTCATGA TTCTTATCAC GGATGGGAAA 
TCATCAGATG CTTTCAGAGA TCCTGCGATA AAACTGAGGA ATTCAGATGT TGAAATCTTT 
GCAGTTGGTG TGAAGGATGC CGTTCGCTCA GAATTGGAAG CTATTGCCTC TCCTCCTGCA 
GAGACCCATG TGTTCACAGT GGAAGATTTT GATGCTTTTC AGAGGATATC TTTTGAACTC 
ACACAGTCTA TCTGCCTTAG AATTGAGCAA GAATTGGCAG CTATAAAGAA GAAAGCTTAC 
GTCCCTCCAA AGGATCTTAG TTTTTCAGAA GTGACTTCTT ATGGTTTCAA AACCAACTGG 
TCTCCAGCTG GAGAAAATGT TTTTTCATAT CACATCACCT ACAAGGAAGC GGCTGGGGAT 
GATGAGGTCA CTGTGGTGGA GCCAGCATCG AGCACCAGTG TTGTTCTCAG CAGCCTGAAG 
CCAGAGACCT TGTATTTGGT CAATGTGACT GCGGAGTATG AGGATGGCTT CAGCATTCCC 
TTAGCTGGAG AGGAGACCAC CGAAGAAGTA AAAGGAGCAC CTCGAAACCT AAAGGTGACA 
GATGAGACTA CAGATAGTTT CAAAATTACT TGGACTCAAG CTCCAGGGAG AGTTTTAAGA 
TATCGAATTA TATATAGACC AGTTGCTGGT GGAGAGAGCA GAGAAGTTAC CACCCCACCC 
AATCAGAGGA GGAGAACACT GGAGAACTTG ATTCCAGACA CGAAATATGA AGTATCTGTA 
ATTCCTGAAT ATTTCTCAGG ACCTGGTACT CCATTAACTG GAAATGCAGC CACTGAAGAA 
GTTAGAGGGA ATCCAAGAGA CTTAAGAGTT TCTGACCCTA CGACGTCTAC TATGAAATTA 
TCTTGGAGTG GGGCACCAGG AAAAGTGAAA CAGTATCTCG TCACATATAC CCCAGTGGCA 
GGGGGTGAAA CTCAAGAGGT CACTGTGAGG GGAGATACAA CCAATACGGT GCTGCAGGGA 
TTGAAGGAAG GGACACAATA CGCCTTATCT GTGACAGCCT TGTATGCGTC TGGGGCTGGA 
GACGCCCTCT TTGGTGAAGG AACAACACTT GAAGAACGTG GTTCTCCTCA AGATTTAGTT 
ACTAAAGACA TCACTGACAC ATCAATTGGG GCTTATTGGA CATCTGCTCC AGGAATGGTT 
CGCGGTTACA GGGTCTCATG GAAATCACTT TATGATGATG TTGACACTGG AGAGAAAAAT 
CTGCCTGAAG ATGCAATTCA TACGATGATA GAAAATCTGC AGCCAGAGAC CAAATACAGA 
ATTTCAGTAT TTGCCACTTA CAGCAGTGGA GAAGGAGAAC CTTTGACTGG AGATGCCACA 
ACTGAATTAT CTCAAGATTC CAAAACCCTG AAAGTAGATG AAGAAACAGA AAACACAATG 
AGAGTTACAT GGAAACCAGC ACCAGGGAAA GTCGTCAACT ACCGTGTTGT CTATCGCCCT 
CATGGGAGAG GGAAGCAAAT GGTTGCTAAG GTGCCCCCCA CAGTCACTTC GACAGTGTTA 
AAGCGACTTC AGCCACAGAC CACATATGAC ATCACAGTTC TTCCTATTTA CAAGATGGGA 
GAAGGAAAGC TTAGGCAAGG ATCAGGAACA ACAGCTTCTC GGTTTAAGTC TCCTAGAAAC 
CTCAAAACAT CTGACCCAAC CATGTCAAGC TTCCGAGTGA CTTGGGAGCC TGCCCCTGGG 
GAAGTGAAGG GTTATAAAGT CACATTCCAC CCTACGGGGG ATGACAGAAG ACTGGGGGAG 
TTAGTGGTTG GACCCTATGA CAACACAGTT GTTTTGGAGG AACTTAGGGC TGGTACCACC 
TATAAAGTAA ATGTTTTTGG AATGTTTGAT GGAGGAGAAA GCTCACCACT TGTTGGACAA 
GAAATGACAA CCCTTTCCGA CACAACTGTT ATGCCAATTT TATCTTCTGG GATGGAGTGT 
CTCACCAGAG CTGAGGCAGA CATTGTGTTG CTGGTGGATG GATCATGGAG CATCGGCCGG 
GCAAATTTTA GAACCGTGAG GAGTTTCATT TCTCGTATTG TGGAAGTCTT TGACATTGGC 
CCCAAAAGAG TACAAATTGC TCTTGCTCAG TATAGTGGGG ATCCCAGAAC AGAGTGGCAG 
TTAAATGCAC ACAGAGACAA GAAGAGCTTG TTGCAAGCTG TGGCAAACTT GCCGTACAAA 
GGAGGCAATA CTCTCACAGG CATGGCTTTG AATTTCATTC GCCAACAGAA CTTCAGGACC 
CAAGCTGGCA TGAGACCTCG AGCTCGAAAA ATTGGTGTGC TCATTACTGA TGGAAAATCA 
CAAGACGATG TTGAAGCACC TTCAAAGAAA CTCAAGGATG AGGGAGTGGA GCTGTTTGCT 
ATTGGTATTA AAAATGCTGA TGAAGTCGAA TTAAAGATGA TTGCAACTGA TCCTGATGAT 
ACCCATGCAT ACAATGTGGC AGATTTTGAG TCACTCTCCA GGATAGTGGA TGATCTCACC 
ATTAATTTGT GTAACAGTGT CAAAGGTCCA GGTGATTTGG AAGCACCTTC TAACTTAGTT 
ATTTCTGAGC GAACCCATCG TTCTTTTAGA GTGAGCTGGA CACCACCTTC TGACAGTGTG 
GATCGATATA AGGTGGAATA CTATCCAGTT TCTGGAGGGA AACGTCAAGA ATTTTATGTG 
AGTCGAATGG AAACTAGCAC AGTGCTGAAA GATCTGAAAC CTGAAACTGA ATATGTTGTC 
AATGTGTATT CTGTGGTAGA AGATGAATAT AGTGAGCCTC TGAAGGGGAC AGAAAAAACC 
TTGCCAGTGC CTGTAGTCAG CCTGAATATT TATGATGTTG GCCCTACCAC CATGCATGTG 
CAGTGGCAGC CTGTGGGAGG AGCTACTGGC TACATCTTGT CATACAAACC TGTTAAGGAC 
ACAGAGCCAA CAAGACCCAA AGAGGTGCGT TTGGGGCCAA CAGTGAATGA CATGCAGCTG 
ACTGACCTTG TTCCCAACAC GGAGTATGCA GTCACAGTCC AGGCTGTCCT GCACGACCTC 
ACTAGTGAAC CTGTCACTGT TCGGGAAGTC ACCTTGCCTT TACCCAGACC TCAGGATCTG 
AAACTCAGAG ATGTGACTCA CAGCACTATG AATGTCTTTT GGGAACCTGT GCCTGGAAAA 
GTGCGTAAAT ATATTGTTCG ATACAAAACA CCAGAAGAGG ATGTCAAAGA GGTAGAGGTG 
GACAGATCAG AGACCAGCAC TTCCCTCAAA GACCTCTTCT CACAGACCTT GTACACAGTC 
AGCGTTTCTG CAGTACATGA CGAGGGGGAG TCTCCTCCAG TGACTGCTCA AGAAACTACC 
CGACCCGTGC CAGCCCCAAC AAACTTAAAG ATTACTGAAG TAACATCAGA GGGTTTCAGA 
GGGACTTGGG ATCATGGAGC TTCAGATGTG TCTCTCTACA GAATAACTTG GGCACCTTTT 
GGAAGCTCAG ATAAGATGGA GACCATCTTA AATGGAGATG AAAACACTTT GGTGTTCGAA 
AACCTGAACC CCAACACCAT CTATGAAGTT TCCATTACTG CCATCTATCC TGATGAGTCA 
GAAAGTGATG ACCTGATTGG CAGTGAGCGC ACACTGCCTA TCTTAACAAC ACAAGCTCCC 
AAAAGTGGCC CACGAAACCT TCAAGTGTAC AATGCAACAT CTAACAGCCT GACTGTTAAG 
TGGGATCCTG CTAGTGGTCG TGTGCAGAAA TATAGGATCA CTTATCAGCC TTCCACAGGG 
GAAGGCAATG AGCAAACGAC CACAATAGGA GGACGGCAGA ACAGTGTGGT CCTGCAGAAA 
CTGAAGCCAG ACACTCCTTA CACTATCACC GTATCCTCTC TGTATCCTGA TGGTGAAGGA 
GGTCGGATGA CGGGAAGAGG CAAGACCAAA CCTCTAAACA CTGTAAGGAA CCTGAGAGTG 
TATGACCCTT CTACCAGCAC CTTGAATGTC CGCTGGGACC ATGCAGAGGG AAATCCTCGT 
CAGTACAAGC TCTTCTATGC ACCAGCAGCA GGTGGTCCAG AGGAACTGGT ACCAATCCCC 
GGGAATACCA ATTATGCCAT TCTTAGGAAT CTGCAGCCAG ATACCTCATA CACTGTGACT 
GTAGTTCCCG TTTATACTGA AGGTGATGGG GGACGCACAT CAGATACTGG AAGGACATTG 
ATGAGAGGAC TGGCAAGAAA TGTCCAAGTA TACAATCCTA CACCTAACAG CCTCGATGTT 
CGCTGGGACC CTGCTCCAGG ACCTGTGCTG CAATATCGCG TTGTGTATTC TCCTGTGGAT 
GGCACAAGAC CCTCAGAATC TATAGTAGTG CCAGGAAACA CGCGCATGGT GCATCTGGAG 
CGGCTGATTC CGGACACACT CTATTCCGTG AACCTTGTGG CTCTGTACTC GGATGGAGAG 
GGAAATCCCA GCCCTGCCCA GGGCCGAACG CTACCACGCA GTGGACCAAG GAACCTGAGA 
GTCTTTGGTG AAACAACCAA TAGCCTCTCG GTAGCCTGGG ATCATGCTGA TGGGCCAGTT 
CAGCAGTACA GGATCATCTA TTCTCCCACT GTTGGTGATC CAATTGATGA ATATACCACA 
GTCCCAGGCA GAAGAAACAA TGTAATACTG CAGCCCCTGC AACCTGACAC TCCATATAAA 
ATTACTGTTA TTGCTGTTTA TGAAGATGGA GATGGTGGCC ATCTAACAGG AAATGGAAGA 
ACTGTGGGAC TCCTTCCTCC TCAGAACATA CACATCTCTG ACGAATGGTA TACAAGATTC 
AGGGTGTCCT GGGATCCTTC ACCTTCTCCA GTTCTTGGAT ATAAAATAGT ATATAAGCCA 
GTGGGTTCCA ATGAGCCCAT GGAAGCCTTT GTTGGAGAAA TGACATCATA TACCTTACAC 
AATCTCAATC CCAGCACCAC CTACGATGTG AATGTTTATG CTCAATATGA TTCTGGACTC 
AGTGTCCCCT TGACAGATCA AGGCACTACA TTATATTTAA ATGTAACAGA TCTGAAAACT 
TACCAGATTG GGTGGGATAC ATTCTGTGTC AAATGGTCAC CTCACCGGGC AGCCACCTCC 
TACAGGCTAA AACTAAGCCC TGCAGATGGA ACAAGGGGAC AAGAAATTAC AGTGCGTGGA 
TCAGAAACCA GTCACTGCTT CACTGGCCTT TCACCAGACA CTGATTATGG TGTCACTGTT 
TTTGTGCAGA CACCAAATCT CGAGGGACCA GGAGTCTCTG TTAAAGAACA TACCACTGTG 
AAACCAACAG AAGCCCCTAC AGAGCCACCC ACACCTCCTC CCCCTCCCAC CATTCCACCA 
GCCCGGGATG TATGCAAAGG GGCCAAGGCA GATATTGTGT TCTTGACTGA TGCCTCCTGG 
AGCATTGGGG ACGATAATTT TAACAAAGTT GTAAAATTCA TCTTCAATAC TGTGGGAGGC 
TTTGATGAAA TCAGTCCTGC TGGGATTCAG GTTTCATTTG TGCAATACAG CGATGAGGTC 
AAGTCTGAGT TCAAGCTGAA CACGTACAAT GACAAGGCCC TAGCCCTTGG GGCCCTCCAG 
AATATTAGGT ACAGAGGAGG AAACACAAGA ACAGGCAAGG CCCTCACGTT TATCAAGGAG 
AAAGTCTTGA CTTGGGAGAG CGGCATGAGG AAGAATGTCC CTAAGGTGTT GGTTGTGGTC 
ACGGACGGTC GGTCCCAGGA TGAGGTCAAG AAGGCGGCTT TGGTCATCCA GCAGTCAGGG 
TTCAGTGTCT TTGTAGTTGG TGTGGCTGAT GTCGACTACA ATGAGCTTGC CAACATTGCC 
AGCAAACCAA GTGAACGGCA CGTGTTCATT GTGGACGACT TTGAATCTTT TGAGAAGATC 
GAAGACAATC TTATTACATT TGTTTGTGAA ACTGCCACTT CAAGTTGTCC TCTCATTTAT 
TTGGATGGCT ACACCTCACC AGGTTTTAAA ATGCTTGAAG CATACAACCT GACAGAAAAG 
AATTTTGCTT CTGTACAAGG AGTATCTTTG GAGTCAGGGT CTTTCCCCAG CTACTCAGCA 
TACAGGATTC AGAAGAATGC GTTTGTGAAT CAGCCTACAG CAGACCTACA CCCAAATGGA 
CTCCCTCCTT CATACACGAT TATATTATTA TTCAGACTTC TCCCAGAAAC TCCCAGTGAC 
CCTTTTGCAA TTTGGCAAAT CACAGACAGA GACTACAAAC CACAAGTTGG AGTGATTGCA 
GATCCTTCTA GCAAGACGTT ATCATTCTTT AACAAGGATA CAAGAGGCGA GGTGCAAACT 
GTTACATTTG ACACAGAAGA AGTAAAGACA TTATTTTATG GAAGTTTTCA CAAGGTTCAT 
ATTGTAGTGA CCTCAAAAAG TGTTAAGATT TACATTGACT GCTATGAAAT TATAGAAAAA 
GACATCAAGG AAGCTGGAAA TATAACAACT GATGGTTATG AAATTCTTGG AAAACTCCTT 
AAAGGGGAAA GGAAATCAGC CGCATTCCAA ATCCAGAGTT TTGACATTGT CTGCAGTCCA 
GTGTGGACCA GTAGAGACAG ATGCTGTGAT ATTCCCTCTA GGAGAGATGA GGGAAAATGC 
CCTGCTTTTC CAAATTCTTG CACATGTACA CAGGACAGCG TTGGACCTCC AGGACCTCCA 
GGCCCTGCAG GAGGACCTGG TGCTAAAGGT CCCAGAGGTG AAAGAGGTAT CAGTGGGGCA 
ATTGGGCCCC CTGGTCCTCG TGGAGACATA GGTCCTCCAG GCCCCCAGGG TCCTCCAGGC 
CCTCAGGGAC CCAATGGACT CTCTATTCCG GGAGAGCAAG GTCGCCAAGG GATGAAAGGT 
GATGCTGGAG AGCCAGGACT TCCAGGCCGA ACAGGAACCC CAGGATTACC TGGCCCACCA 
GGACCAATGG GACCTCCAGG AGACAGAGGC TTCACTGGAA AAGACGGTGC AATGGGACCC 
AGGGGCCCAC CAGGGCCGCC GGGAAGCCCA GGCTCCCCAG GAGTCACAGG ACCAAGTGGG 
AAGCCAGGAA AACCTGGAGA TCATGGCAGA CCAGGTCCAT CTGGGTTGAA AGGAGAAAAA 
GGTGATAGGG GAGACATTGC TTCCCAGAAC ATGATGCGAG CAGTTGCAAG ACAAGTCTGT 
GAACAATTGA TAAGTGGTCA GATGAACAGA TTCAATCAGA TGCTGAATCA GATTCCAAAT 
GATTACCAGT CCAGTCGCAA CCAGCCAGGC CCGCCGGGTC CACCGGGACC TCCTGGTAGC 
GCAGGAGCCA GAGGAGAACC TGGGCCTGGG GGGCGGCCAG GCTTCCCGGG CACACCAGGG 
ATGCAGGGAC CCCCTGGGGA ACGAGGTTTG CCAGGAGAGA AAGGTGAAAG GGGTACTGGA 
TCTTCAGGAC CTCGGGGGCT GCCTGGCCCC CCAGGTCCAC AAGGAGAATC CAGAACAGGT 
CCACCAGGGT CCACAGGTTC AAGAGGTCCC CCTGGCCCCC CTGGCCGTCC TGGAAACTCA 
GGTATCCGAG GACCCCCAGG TCCTCCTGGA TACTGTGATT CTTCTCAGTG TGCCAGCATC 
CCATACAACG GGCAAGGCTA TCCAGAGCCT TACGTGCCCG AGGGCGGGGC CTACCTGCCT 
GAGCGCGAGC CCTTCATCGT GCCGGTGGAG CCCGAGCGGA CAGCGGAATA CGAGGACTAC 
GGCGCAGACG AGCCTGCAGA GGAGCCTCCT GAGCCCCACC GGCGCTGGCG GCGCGCCCTG 
CCCCACGGAC CCGGGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL12A1 ( XM_017010252.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865741.1 Homo sapiens collagen type XII alpha 1 (COL12A1), transcript variant X2, mRNA.

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CloneID	OHu96293
Clone ID Related Accession (Same CDS sequence)	XM_017010252.2 , XM_017010252.1
Accession Version	XM_017010252.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(XII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAACA GACAAGACCC CAGTCTTCTA GTCCTAGTTG ACCCACCTTC AGACTTGAAT 
TTTAAAATTA TAGATGAAAA TACTGTTCAT ATGTCATGGG CAAAACCAGT TGATCCAATT 
GTGGGTTACA GAATAACGGT GGACCCTACA ACGGATGGGC CTACTAAAGA ATTTACCCTT 
TCAGCTAGTA CCACTGAAAC TTTATTGTCA GAACTTGTAC CTGAAACAGA GTATGTGGTG 
ACAATAACTT CATATGATGA AGTAGAAGAA AGTGTACCAG TTATAGGACA ACTAACAATT 
CAAACAGGTA GTTCGACAAA GCCAGTGGAG AAGAAACCTG GAAAAACCGA GATACAAAAA 
TGCTCTGTCA GTGCCTGGAC TGATTTGGTT TTCCTCGTGG ATGGCTCTTG GAGTGTGGGA 
AGAAATAATT TCAAGTACAT TTTAGACTTC ATTGCTGCTC TTGTGTCTGC TTTTGACATT 
GGGGAAGAGA AGACAAGAGT TGGAGTTGTT CAATACAGCT CTGATACCAG GACTGAATTT 
AACTTAAATC AGTACTACCA AAGGGATGAA CTTCTTGCTG CAATAAAAAA AATTCCATAT 
AAAGGTGGCA ACACAATGAC AGGGGATGCC ATTGATTATT TAGTTAAAAA TACTTTCACG 
GAATCTGCTG GGGCAAGAGT TGGCTTTCCT AAAGTGGCAA TTATTATTAC GGATGGAAAA 
TCCCAGGATG AAGTGGAAAT TCCAGCAAGA GAGCTTCGTA ATGTTGGAGT TGAAGTTTTC 
TCCTTGGGCA TTAAAGCTGC AGATGCAAAA GAACTCAAAC AAATTGCCTC CACACCTTCA 
CTGAACCATG TTTTCAATGT GGCCAACTTT GATGCAATTG TGGATATTCA GAATGAGATC 
ATCTCCCAGG TGTGCTCAGG TGTTGATGAA CAACTTGGTG AATTGGTTAG TGGAGAAGAA 
GTTGTTGAGC CTCCTTCAAA TTTGATTGCC ATGGAAGTCT CTTCAAAATA TGTTAAGCTA 
AATTGGAATC CATCTCCTAG TCCAGTGACT GGCTACAAAG TCATCCTCAC ACCAATGACT 
GCAGGAAGCC GACAGCACGC TCTGAGTGTG GGGCCTCAGA CAACCACGCT CAGTGTTCGC 
GACCTCTCAG CAGACACAGA ATACCAGATC AGTGTTTCCG CCATGAAGGG AATGACATCC 
AGTGAACCCA TTTCAATAAT GGAGAAGACT CAGCCAATGA AAGTTCAAGT GGAATGCTCA 
CGTGGTGTGG ATATAAAAGC CGATATTGTG TTTTTGGTTG ATGGCTCCTA TAGCATTGGG 
ATTGCAAACT TTGTTAAAGT TAGAGCCTTT TTGGAAGTTC TTGTAAAAAG TTTTGAAATT 
TCACCAAATA GGGTCCAGAT TAGTCTTGTG CAATACAGCC GGGATCCTCA TACTGAGTTC 
ACTTTGAAAA AATTCACCAA AGTTGAAGAT ATAATTGAAG CAATAAACAC CTTCCCTTAC 
AGAGGAGGAT CTACAAATAC TGGCAAAGCA ATGACTTATG TCAGAGAGAA AATATTTGTG 
CCTAGCAAGG GATCAAGAAG CAATGTGCCA AAGGTCATGA TTCTTATCAC GGATGGGAAA 
TCATCAGATG CTTTCAGAGA TCCTGCGATA AAACTGAGGA ATTCAGATGT TGAAATCTTT 
GCAGTTGGTG TGAAGGATGC CGTTCGCTCA GAATTGGAAG CTATTGCCTC TCCTCCTGCA 
GAGACCCATG TGTTCACAGT GGAAGATTTT GATGCTTTTC AGAGGATATC TTTTGAACTC 
ACACAGTCTA TCTGCCTTAG AATTGAGCAA GAATTGGCAG CTATAAAGAA GAAAGCTTAC 
GTCCCTCCAA AGGATCTTAG TTTTTCAGAA GTGACTTCTT ATGGTTTCAA AACCAACTGG 
TCTCCAGCTG GAGAAAATGT TTTTTCATAT CACATCACCT ACAAGGAAGC GGCTGGGGAT 
GATGAGGTCA CTGTGGTGGA GCCAGCATCG AGCACCAGTG TTGTTCTCAG CAGCCTGAAG 
CCAGAGACCT TGTATTTGGT CAATGTGACT GCGGAGTATG AGGATGGCTT CAGCATTCCC 
TTAGCTGGAG AGGAGACCAC CGAAGAAGTA AAAGGAGCAC CTCGAAACCT AAAGGTGACA 
GATGAGACTA CAGATAGTTT CAAAATTACT TGGACTCAAG CTCCAGGGAG AGTTTTAAGA 
TATCGAATTA TATATAGACC AGTTGCTGGT GGAGAGAGCA GAGAAGTTAC CACCCCACCC 
AATCAGAGGA GGAGAACACT GGAGAACTTG ATTCCAGACA CGAAATATGA AGTATCTGTA 
ATTCCTGAAT ATTTCTCAGG ACCTGGTACT CCATTAACTG GAAATGCAGC CACTGAAGAA 
GTTAGAGGGA ATCCAAGAGA CTTAAGAGTT TCTGACCCTA CGACGTCTAC TATGAAATTA 
TCTTGGAGTG GGGCACCAGG AAAAGTGAAA CAGTATCTCG TCACATATAC CCCAGTGGCA 
GGGGGTGAAA CTCAAGAGGT CACTGTGAGG GGAGATACAA CCAATACGGT GCTGCAGGGA 
TTGAAGGAAG GGACACAATA CGCCTTATCT GTGACAGCCT TGTATGCGTC TGGGGCTGGA 
GACGCCCTCT TTGGTGAAGG AACAACACTT GAAGAACGTG GTTCTCCTCA AGATTTAGTT 
ACTAAAGACA TCACTGACAC ATCAATTGGG GCTTATTGGA CATCTGCTCC AGGAATGGTT 
CGCGGTTACA GGGTCTCATG GAAATCACTT TATGATGATG TTGACACTGG AGAGAAAAAT 
CTGCCTGAAG ATGCAATTCA TACGATGATA GAAAATCTGC AGCCAGAGAC CAAATACAGA 
ATTTCAGTAT TTGCCACTTA CAGCAGTGGA GAAGGAGAAC CTTTGACTGG AGATGCCACA 
ACTGAATTAT CTCAAGATTC CAAAACCCTG AAAGTAGATG AAGAAACAGA AAACACAATG 
AGAGTTACAT GGAAACCAGC ACCAGGGAAA GTCGTCAACT ACCGTGTTGT CTATCGCCCT 
CATGGGAGAG GGAAGCAAAT GGTTGCTAAG GTGCCCCCCA CAGTCACTTC GACAGTGTTA 
AAGCGACTTC AGCCACAGAC CACATATGAC ATCACAGTTC TTCCTATTTA CAAGATGGGA 
GAAGGAAAGC TTAGGCAAGG ATCAGGAACA ACAGCTTCTC GGTTTAAGTC TCCTAGAAAC 
CTCAAAACAT CTGACCCAAC CATGTCAAGC TTCCGAGTGA CTTGGGAGCC TGCCCCTGGG 
GAAGTGAAGG GTTATAAAGT CACATTCCAC CCTACGGGGG ATGACAGAAG ACTGGGGGAG 
TTAGTGGTTG GACCCTATGA CAACACAGTT GTTTTGGAGG AACTTAGGGC TGGTACCACC 
TATAAAGTAA ATGTTTTTGG AATGTTTGAT GGAGGAGAAA GCTCACCACT TGTTGGACAA 
GAAATGACAA CCCTTTCCGA CACAACTGTT ATGCCAATTT TATCTTCTGG GATGGAGTGT 
CTCACCAGAG CTGAGGCAGA CATTGTGTTG CTGGTGGATG GATCATGGAG CATCGGCCGG 
GCAAATTTTA GAACCGTGAG GAGTTTCATT TCTCGTATTG TGGAAGTCTT TGACATTGGC 
CCCAAAAGAG TACAAATTGC TCTTGCTCAG TATAGTGGGG ATCCCAGAAC AGAGTGGCAG 
TTAAATGCAC ACAGAGACAA GAAGAGCTTG TTGCAAGCTG TGGCAAACTT GCCGTACAAA 
GGAGGCAATA CTCTCACAGG CATGGCTTTG AATTTCATTC GCCAACAGAA CTTCAGGACC 
CAAGCTGGCA TGAGACCTCG AGCTCGAAAA ATTGGTGTGC TCATTACTGA TGGAAAATCA 
CAAGACGATG TTGAAGCACC TTCAAAGAAA CTCAAGGATG AGGGAGTGGA GCTGTTTGCT 
ATTGGTATTA AAAATGCTGA TGAAGTCGAA TTAAAGATGA TTGCAACTGA TCCTGATGAT 
ACCCATGCAT ACAATGTGGC AGATTTTGAG TCACTCTCCA GGATAGTGGA TGATCTCACC 
ATTAATTTGT GTAACAGTGT CAAAGGTCCA GGTGATTTGG AAGCACCTTC TAACTTAGTT 
ATTTCTGAGC GAACCCATCG TTCTTTTAGA GTGAGCTGGA CACCACCTTC TGACAGTGTG 
GATCGATATA AGGTGGAATA CTATCCAGTT TCTGGAGGGA AACGTCAAGA ATTTTATGTG 
AGTCGAATGG AAACTAGCAC AGTGCTGAAA GATCTGAAAC CTGAAACTGA ATATGTTGTC 
AATGTGTATT CTGTGGTAGA AGATGAATAT AGTGAGCCTC TGAAGGGGAC AGAAAAAACC 
TTGCCAGTGC CTGTAGTCAG CCTGAATATT TATGATGTTG GCCCTACCAC CATGCATGTG 
CAGTGGCAGC CTGTGGGAGG AGCTACTGGC TACATCTTGT CATACAAACC TGTTAAGGAC 
ACAGAGCCAA CAAGACCCAA AGAGGTGCGT TTGGGGCCAA CAGTGAATGA CATGCAGCTG 
ACTGACCTTG TTCCCAACAC GGAGTATGCA GTCACAGTCC AGGCTGTCCT GCACGACCTC 
ACTAGTGAAC CTGTCACTGT TCGGGAAGTC ACCTTGCCTT TACCCAGACC TCAGGATCTG 
AAACTCAGAG ATGTGACTCA CAGCACTATG AATGTCTTTT GGGAACCTGT GCCTGGAAAA 
GTGCGTAAAT ATATTGTTCG ATACAAAACA CCAGAAGAGG ATGTCAAAGA GGTAGAGGTG 
GACAGATCAG AGACCAGCAC TTCCCTCAAA GACCTCTTCT CACAGACCTT GTACACAGTC 
AGCGTTTCTG CAGTACATGA CGAGGGGGAG TCTCCTCCAG TGACTGCTCA AGAAACTACC 
CGACCCGTGC CAGCCCCAAC AAACTTAAAG ATTACTGAAG TAACATCAGA GGGTTTCAGA 
GGGACTTGGG ATCATGGAGC TTCAGATGTG TCTCTCTACA GAATAACTTG GGCACCTTTT 
GGAAGCTCAG ATAAGATGGA GACCATCTTA AATGGAGATG AAAACACTTT GGTGTTCGAA 
AACCTGAACC CCAACACCAT CTATGAAGTT TCCATTACTG CCATCTATCC TGATGAGTCA 
GAAAGTGATG ACCTGATTGG CAGTGAGCGC ACACTGCCTA TCTTAACAAC ACAAGCTCCC 
AAAAGTGGCC CACGAAACCT TCAAGTGTAC AATGCAACAT CTAACAGCCT GACTGTTAAG 
TGGGATCCTG CTAGTGGTCG TGTGCAGAAA TATAGGATCA CTTATCAGCC TTCCACAGGG 
GAAGGCAATG AGCAAACGAC CACAATAGGA GGACGGCAGA ACAGTGTGGT CCTGCAGAAA 
CTGAAGCCAG ACACTCCTTA CACTATCACC GTATCCTCTC TGTATCCTGA TGGTGAAGGA 
GGTCGGATGA CGGGAAGAGG CAAGACCAAA CCTCTAAACA CTGTAAGGAA CCTGAGAGTG 
TATGACCCTT CTACCAGCAC CTTGAATGTC CGCTGGGACC ATGCAGAGGG AAATCCTCGT 
CAGTACAAGC TCTTCTATGC ACCAGCAGCA GGTGGTCCAG AGGAACTGGT ACCAATCCCC 
GGGAATACCA ATTATGCCAT TCTTAGGAAT CTGCAGCCAG ATACCTCATA CACTGTGACT 
GTAGTTCCCG TTTATACTGA AGGTGATGGG GGACGCACAT CAGATACTGG AAGGACATTG 
ATGAGAGGAC TGGCAAGAAA TGTCCAAGTA TACAATCCTA CACCTAACAG CCTCGATGTT 
CGCTGGGACC CTGCTCCAGG ACCTGTGCTG CAATATCGCG TTGTGTATTC TCCTGTGGAT 
GGCACAAGAC CCTCAGAATC TATAGTAGTG CCAGGAAACA CGCGCATGGT GCATCTGGAG 
CGGCTGATTC CGGACACACT CTATTCCGTG AACCTTGTGG CTCTGTACTC GGATGGAGAG 
GGAAATCCCA GCCCTGCCCA GGGCCGAACG CTACCACGCA GTGGACCAAG GAACCTGAGA 
GTCTTTGGTG AAACAACCAA TAGCCTCTCG GTAGCCTGGG ATCATGCTGA TGGGCCAGTT 
CAGCAGTACA GGATCATCTA TTCTCCCACT GTTGGTGATC CAATTGATGA ATATACCACA 
GTCCCAGGCA GAAGAAACAA TGTAATACTG CAGCCCCTGC AACCTGACAC TCCATATAAA 
ATTACTGTTA TTGCTGTTTA TGAAGATGGA GATGGTGGCC ATCTAACAGG AAATGGAAGA 
ACTGTGGGAC TCCTTCCTCC TCAGAACATA CACATCTCTG ACGAATGGTA TACAAGATTC 
AGGGTGTCCT GGGATCCTTC ACCTTCTCCA GTTCTTGGAT ATAAAATAGT ATATAAGCCA 
GTGGGTTCCA ATGAGCCCAT GGAAGCCTTT GTTGGAGAAA TGACATCATA TACCTTACAC 
AATCTCAATC CCAGCACCAC CTACGATGTG AATGTTTATG CTCAATATGA TTCTGGACTC 
AGTGTCCCCT TGACAGATCA AGGCACTACA TTATATTTAA ATGTAACAGA TCTGAAAACT 
TACCAGATTG GGTGGGATAC ATTCTGTGTC AAATGGTCAC CTCACCGGGC AGCCACCTCC 
TACAGGCTAA AACTAAGCCC TGCAGATGGA ACAAGGGGAC AAGAAATTAC AGTGCGTGGA 
TCAGAAACCA GTCACTGCTT CACTGGCCTT TCACCAGACA CTGATTATGG TGTCACTGTT 
TTTGTGCAGA CACCAAATCT CGAGGGACCA GGAGTCTCTG TTAAAGAACA TACCACTGTG 
AAACCAACAG AAGCCCCTAC AGAGCCACCC ACACCTCCTC CCCCTCCCAC CATTCCACCA 
GCCCGGGATG TATGCAAAGG GGCCAAGGCA GATATTGTGT TCTTGACTGA TGCCTCCTGG 
AGCATTGGGG ACGATAATTT TAACAAAGTT GTAAAATTCA TCTTCAATAC TGTGGGAGGC 
TTTGATGAAA TCAGTCCTGC TGGGATTCAG GTTTCATTTG TGCAATACAG CGATGAGGTC 
AAGTCTGAGT TCAAGCTGAA CACGTACAAT GACAAGGCCC TAGCCCTTGG GGCCCTCCAG 
AATATTAGGT ACAGAGGAGG AAACACAAGA ACAGGCAAGG CCCTCACGTT TATCAAGGAG 
AAAGTCTTGA CTTGGGAGAG CGGCATGAGG AAGAATGTCC CTAAGGTGTT GGTTGTGGTC 
ACGGACGGTC GGTCCCAGGA TGAGGTCAAG AAGGCGGCTT TGGTCATCCA GCAGTCAGGG 
TTCAGTGTCT TTGTAGTTGG TGTGGCTGAT GTCGACTACA ATGAGCTTGC CAACATTGCC 
AGCAAACCAA GTGAACGGCA CGTGTTCATT GTGGACGACT TTGAATCTTT TGAGAAGATC 
GAAGACAATC TTATTACATT TGTTTGTGAA ACTGCCACTT CAAGTTGTCC TCTCATTTAT 
TTGGATGGCT ACACCTCACC AGGTTTTAAA ATGCTTGAAG CATACAACCT GACAGAAAAG 
AATTTTGCTT CTGTACAAGG AGTATCTTTG GAGTCAGGGT CTTTCCCCAG CTACTCAGCA 
TACAGGATTC AGAAGAATGC GTTTGTGAAT CAGCCTACAG CAGACCTACA CCCAAATGGA 
CTCCCTCCTT CATACACGAT TATATTATTA TTCAGACTTC TCCCAGAAAC TCCCAGTGAC 
CCTTTTGCAA TTTGGCAAAT CACAGACAGA GACTACAAAC CACAAGTTGG AGTGATTGCA 
GATCCTTCTA GCAAGACGTT ATCATTCTTT AACAAGGATA CAAGAGGCGA GGTGCAAACT 
GTTACATTTG ACACAGAAGA AGTAAAGACA TTATTTTATG GAAGTTTTCA CAAGGTTCAT 
ATTGTAGTGA CCTCAAAAAG TGTTAAGATT TACATTGACT GCTATGAAAT TATAGAAAAA 
GACATCAAGG AAGCTGGAAA TATAACAACT GATGGTTATG AAATTCTTGG AAAACTCCTT 
AAAGGGGAAA GGAAATCAGC CGCATTCCAA ATCCAGAGTT TTGACATTGT CTGCAGTCCA 
GTGTGGACCA GTAGAGACAG ATGCTGTGAT ATTCCCTCTA GGAGAGATGA GGGAAAATGC 
CCTGCTTTTC CAAATTCTTG CACATGTACA CAGGACAGCG TTGGACCTCC AGGACCTCCA 
GGCCCTGCAG GAGGACCTGG TGCTAAAGGT CCCAGAGGTG AAAGAGGTAT CAGTGGGGCA 
ATTGGGCCCC CTGGTCCTCG TGGAGACATA GGTCCTCCAG GCCCCCAGGG TCCTCCAGGC 
CCTCAGGGAC CCAATGGACT CTCTATTCCG GGAGAGCAAG GTCGCCAAGG GATGAAAGGT 
GATGCTGGAG AGCCAGGACT TCCAGGCCGA ACAGGAACCC CAGGATTACC TGGCCCACCA 
GGACCAATGG GACCTCCAGG AGACAGAGGC TTCACTGGAA AAGACGGTGC AATGGGACCC 
AGGGGCCCAC CAGGGCCGCC GGGAAGCCCA GGCTCCCCAG GAGTCACAGG ACCAAGTGGG 
AAGCCAGGAA AACCTGGAGA TCATGGCAGA CCAGGTCCAT CTGGGTTGAA AGGAGAAAAA 
GGTGATAGGG GAGACATTGC TTCCCAGAAC ATGATGCGAG CAGTTGCAAG ACAAGTCTGT 
GAACAATTGA TAAGTGGTCA GATGAACAGA TTCAATCAGA TGCTGAATCA GATTCCAAAT 
GATTACCAGT CCAGTCGCAA CCAGCCAGGC CCGCCGGGTC CACCGGGACC TCCTGGTAGC 
GCAGGAGCCA GAGGAGAACC TGGGCCTGGG GGGCGGCCAG GCTTCCCGGG CACACCAGGG 
ATGCAGGGAC CCCCTGGGGA ACGAGGTTTG CCAGGAGAGA AAGGTGAAAG GGGTACTGGA 
TCTTCAGGAC CTCGGGGGCT GCCTGGCCCC CCAGGTCCAC AAGGAGAATC CAGAACAGGT 
CCACCAGGGT CCACAGGTTC AAGAGGTCCC CCTGGCCCCC CTGGCCGTCC TGGAAACTCA 
GGTATCCGAG GACCCCCAGG TCCTCCTGGA TACTGTGATT CTTCTCAGTG TGCCAGCATC 
CCATACAACG GGCAAGGCTA TCCAGAGCCT TACGTGCCCG AGGGCGGGGC CTACCTGCCT 
GAGCGCGAGC CCTTCATCGT GCCGGTGGAG CCCGAGCGGA CAGCGGAATA CGAGGACTAC 
GGCGCAGACG AGCCTGCAGA GGAGCCTCCT GAGCCCCACC GGCGCTGGCG GCGCGCCCTG 
CCCCACGGAC CCGGGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865741.1
CDS	599..9919
Translation	MKNRQDPSLLVLVDPPSDLNFKIIDENTVHMSWAKPVDPIVGYRITVDPTTDGPTKEFTLSASTTETLLSELVPETEYVVTITSYDEVEESVPVIGQLTIQTGSSTKPVEKKPGKTEIQKCSVSAWTDLVFLVDGSWSVGRNNFKYILDFIAALVSAFDIGEEKTRVGVVQYSSDTRTEFNLNQYYQRDELLAAIKKIPYKGGNTMTGDAIDYLVKNTFTESAGARVGFPKVAIIITDGKSQDEVEIPARELRNVGVEVFSLGIKAADAKELKQIASTPSLNHVFNVANFDAIVDIQNEIISQVCSGVDEQLGELVSGEEVVEPPSNLIAMEVSSKYVKLNWNPSPSPVTGYKVILTPMTAGSRQHALSVGPQTTTLSVRDLSADTEYQISVSAMKGMTSSEPISIMEKTQPMKVQVECSRGVDIKADIVFLVDGSYSIGIANFVKVRAFLEVLVKSFEISPNRVQISLVQYSRDPHTEFTLKKFTKVEDIIEAINTFPYRGGSTNTGKAMTYVREKIFVPSKGSRSNVPKVMILITDGKSSDAFRDPAIKLRNSDVEIFAVGVKDAVRSELEAIASPPAETHVFTVEDFDAFQRISFELTQSICLRIEQELAAIKKKAYVPPKDLSFSEVTSYGFKTNWSPAGENVFSYHITYKEAAGDDEVTVVEPASSTSVVLSSLKPETLYLVNVTAEYEDGFSIPLAGEETTEEVKGAPRNLKVTDETTDSFKITWTQAPGRVLRYRIIYRPVAGGESREVTTPPNQRRRTLENLIPDTKYEVSVIPEYFSGPGTPLTGNAATEEVRGNPRDLRVSDPTTSTMKLSWSGAPGKVKQYLVTYTPVAGGETQEVTVRGDTTNTVLQGLKEGTQYALSVTALYASGAGDALFGEGTTLEERGSPQDLVTKDITDTSIGAYWTSAPGMVRGYRVSWKSLYDDVDTGEKNLPEDAIHTMIENLQPETKYRISVFATYSSGEGEPLTGDATTELSQDSKTLKVDEETENTMRVTWKPAPGKVVNYRVVYRPHGRGKQMVAKVPPTVTSTVLKRLQPQTTYDITVLPIYKMGEGKLRQGSGTTASRFKSPRNLKTSDPTMSSFRVTWEPAPGEVKGYKVTFHPTGDDRRLGELVVGPYDNTVVLEELRAGTTYKVNVFGMFDGGESSPLVGQEMTTLSDTTVMPILSSGMECLTRAEADIVLLVDGSWSIGRANFRTVRSFISRIVEVFDIGPKRVQIALAQYSGDPRTEWQLNAHRDKKSLLQAVANLPYKGGNTLTGMALNFIRQQNFRTQAGMRPRARKIGVLITDGKSQDDVEAPSKKLKDEGVELFAIGIKNADEVELKMIATDPDDTHAYNVADFESLSRIVDDLTINLCNSVKGPGDLEAPSNLVISERTHRSFRVSWTPPSDSVDRYKVEYYPVSGGKRQEFYVSRMETSTVLKDLKPETEYVVNVYSVVEDEYSEPLKGTEKTLPVPVVSLNIYDVGPTTMHVQWQPVGGATGYILSYKPVKDTEPTRPKEVRLGPTVNDMQLTDLVPNTEYAVTVQAVLHDLTSEPVTVREVTLPLPRPQDLKLRDVTHSTMNVFWEPVPGKVRKYIVRYKTPEEDVKEVEVDRSETSTSLKDLFSQTLYTVSVSAVHDEGESPPVTAQETTRPVPAPTNLKITEVTSEGFRGTWDHGASDVSLYRITWAPFGSSDKMETILNGDENTLVFENLNPNTIYEVSITAIYPDESESDDLIGSERTLPILTTQAPKSGPRNLQVYNATSNSLTVKWDPASGRVQKYRITYQPSTGEGNEQTTTIGGRQNSVVLQKLKPDTPYTITVSSLYPDGEGGRMTGRGKTKPLNTVRNLRVYDPSTSTLNVRWDHAEGNPRQYKLFYAPAAGGPEELVPIPGNTNYAILRNLQPDTSYTVTVVPVYTEGDGGRTSDTGRTLMRGLARNVQVYNPTPNSLDVRWDPAPGPVLQYRVVYSPVDGTRPSESIVVPGNTRMVHLERLIPDTLYSVNLVALYSDGEGNPSPAQGRTLPRSGPRNLRVFGETTNSLSVAWDHADGPVQQYRIIYSPTVGDPIDEYTTVPGRRNNVILQPLQPDTPYKITVIAVYEDGDGGHLTGNGRTVGLLPPQNIHISDEWYTRFRVSWDPSPSPVLGYKIVYKPVGSNEPMEAFVGEMTSYTLHNLNPSTTYDVNVYAQYDSGLSVPLTDQGTTLYLNVTDLKTYQIGWDTFCVKWSPHRAATSYRLKLSPADGTRGQEITVRGSETSHCFTGLSPDTDYGVTVFVQTPNLEGPGVSVKEHTTVKPTEAPTEPPTPPPPPTIPPARDVCKGAKADIVFLTDASWSIGDDNFNKVVKFIFNTVGGFDEISPAGIQVSFVQYSDEVKSEFKLNTYNDKALALGALQNIRYRGGNTRTGKALTFIKEKVLTWESGMRKNVPKVLVVVTDGRSQDEVKKAALVIQQSGFSVFVVGVADVDYNELANIASKPSERHVFIVDDFESFEKIEDNLITFVCETATSSCPLIYLDGYTSPGFKMLEAYNLTEKNFASVQGVSLESGSFPSYSAYRIQKNAFVNQPTADLHPNGLPPSYTIILLFRLLPETPSDPFAIWQITDRDYKPQVGVIADPSSKTLSFFNKDTRGEVQTVTFDTEEVKTLFYGSFHKVHIVVTSKSVKIYIDCYEIIEKDIKEAGNITTDGYEILGKLLKGERKSAAFQIQSFDIVCSPVWTSRDRCCDIPSRRDEGKCPAFPNSCTCTQDSVGPPGPPGPAGGPGAKGPRGERGISGAIGPPGPRGDIGPPGPQGPPGPQGPNGLSIPGEQGRQGMKGDAGEPGLPGRTGTPGLPGPPGPMGPPGDRGFTGKDGAMGPRGPPGPPGSPGSPGVTGPSGKPGKPGDHGRPGPSGLKGEKGDRGDIASQNMMRAVARQVCEQLISGQMNRFNQMLNQIPNDYQSSRNQPGPPGPPGPPGSAGARGEPGPGGRPGFPGTPGMQGPPGERGLPGEKGERGTGSSGPRGLPGPPGPQGESRTGPPGSTGSRGPPGPPGRPGNSGIRGPPGPPGYCDSSQCASIPYNGQGYPEPYVPEGGAYLPEREPFIVPVEPERTAEYEDYGADEPAEEPPEPHRRWRRALPHGPGQ

Target ORF information:

RefSeq Version	XM_017010252.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XII alpha 1 (COL12A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010252.1

ORF Insert Sequence:

ATGAAAAACA GACAAGACCC CAGTCTTCTA GTCCTAGTTG ACCCACCTTC AGACTTGAAT 
TTTAAAATTA TAGATGAAAA TACTGTTCAT ATGTCATGGG CAAAACCAGT TGATCCAATT 
GTGGGTTACA GAATAACGGT GGACCCTACA ACGGATGGGC CTACTAAAGA ATTTACCCTT 
TCAGCTAGTA CCACTGAAAC TTTATTGTCA GAACTTGTAC CTGAAACAGA GTATGTGGTG 
ACAATAACTT CATATGATGA AGTAGAAGAA AGTGTACCAG TTATAGGACA ACTAACAATT 
CAAACAGGTA GTTCGACAAA GCCAGTGGAG AAGAAACCTG GAAAAACCGA GATACAAAAA 
TGCTCTGTCA GTGCCTGGAC TGATTTGGTT TTCCTCGTGG ATGGCTCTTG GAGTGTGGGA 
AGAAATAATT TCAAGTACAT TTTAGACTTC ATTGCTGCTC TTGTGTCTGC TTTTGACATT 
GGGGAAGAGA AGACAAGAGT TGGAGTTGTT CAATACAGCT CTGATACCAG GACTGAATTT 
AACTTAAATC AGTACTACCA AAGGGATGAA CTTCTTGCTG CAATAAAAAA AATTCCATAT 
AAAGGTGGCA ACACAATGAC AGGGGATGCC ATTGATTATT TAGTTAAAAA TACTTTCACG 
GAATCTGCTG GGGCAAGAGT TGGCTTTCCT AAAGTGGCAA TTATTATTAC GGATGGAAAA 
TCCCAGGATG AAGTGGAAAT TCCAGCAAGA GAGCTTCGTA ATGTTGGAGT TGAAGTTTTC 
TCCTTGGGCA TTAAAGCTGC AGATGCAAAA GAACTCAAAC AAATTGCCTC CACACCTTCA 
CTGAACCATG TTTTCAATGT GGCCAACTTT GATGCAATTG TGGATATTCA GAATGAGATC 
ATCTCCCAGG TGTGCTCAGG TGTTGATGAA CAACTTGGTG AATTGGTTAG TGGAGAAGAA 
GTTGTTGAGC CTCCTTCAAA TTTGATTGCC ATGGAAGTCT CTTCAAAATA TGTTAAGCTA 
AATTGGAATC CATCTCCTAG TCCAGTGACT GGCTACAAAG TCATCCTCAC ACCAATGACT 
GCAGGAAGCC GACAGCACGC TCTGAGTGTG GGGCCTCAGA CAACCACGCT CAGTGTTCGC 
GACCTCTCAG CAGACACAGA ATACCAGATC AGTGTTTCCG CCATGAAGGG AATGACATCC 
AGTGAACCCA TTTCAATAAT GGAGAAGACT CAGCCAATGA AAGTTCAAGT GGAATGCTCA 
CGTGGTGTGG ATATAAAAGC CGATATTGTG TTTTTGGTTG ATGGCTCCTA TAGCATTGGG 
ATTGCAAACT TTGTTAAAGT TAGAGCCTTT TTGGAAGTTC TTGTAAAAAG TTTTGAAATT 
TCACCAAATA GGGTCCAGAT TAGTCTTGTG CAATACAGCC GGGATCCTCA TACTGAGTTC 
ACTTTGAAAA AATTCACCAA AGTTGAAGAT ATAATTGAAG CAATAAACAC CTTCCCTTAC 
AGAGGAGGAT CTACAAATAC TGGCAAAGCA ATGACTTATG TCAGAGAGAA AATATTTGTG 
CCTAGCAAGG GATCAAGAAG CAATGTGCCA AAGGTCATGA TTCTTATCAC GGATGGGAAA 
TCATCAGATG CTTTCAGAGA TCCTGCGATA AAACTGAGGA ATTCAGATGT TGAAATCTTT 
GCAGTTGGTG TGAAGGATGC CGTTCGCTCA GAATTGGAAG CTATTGCCTC TCCTCCTGCA 
GAGACCCATG TGTTCACAGT GGAAGATTTT GATGCTTTTC AGAGGATATC TTTTGAACTC 
ACACAGTCTA TCTGCCTTAG AATTGAGCAA GAATTGGCAG CTATAAAGAA GAAAGCTTAC 
GTCCCTCCAA AGGATCTTAG TTTTTCAGAA GTGACTTCTT ATGGTTTCAA AACCAACTGG 
TCTCCAGCTG GAGAAAATGT TTTTTCATAT CACATCACCT ACAAGGAAGC GGCTGGGGAT 
GATGAGGTCA CTGTGGTGGA GCCAGCATCG AGCACCAGTG TTGTTCTCAG CAGCCTGAAG 
CCAGAGACCT TGTATTTGGT CAATGTGACT GCGGAGTATG AGGATGGCTT CAGCATTCCC 
TTAGCTGGAG AGGAGACCAC CGAAGAAGTA AAAGGAGCAC CTCGAAACCT AAAGGTGACA 
GATGAGACTA CAGATAGTTT CAAAATTACT TGGACTCAAG CTCCAGGGAG AGTTTTAAGA 
TATCGAATTA TATATAGACC AGTTGCTGGT GGAGAGAGCA GAGAAGTTAC CACCCCACCC 
AATCAGAGGA GGAGAACACT GGAGAACTTG ATTCCAGACA CGAAATATGA AGTATCTGTA 
ATTCCTGAAT ATTTCTCAGG ACCTGGTACT CCATTAACTG GAAATGCAGC CACTGAAGAA 
GTTAGAGGGA ATCCAAGAGA CTTAAGAGTT TCTGACCCTA CGACGTCTAC TATGAAATTA 
TCTTGGAGTG GGGCACCAGG AAAAGTGAAA CAGTATCTCG TCACATATAC CCCAGTGGCA 
GGGGGTGAAA CTCAAGAGGT CACTGTGAGG GGAGATACAA CCAATACGGT GCTGCAGGGA 
TTGAAGGAAG GGACACAATA CGCCTTATCT GTGACAGCCT TGTATGCGTC TGGGGCTGGA 
GACGCCCTCT TTGGTGAAGG AACAACACTT GAAGAACGTG GTTCTCCTCA AGATTTAGTT 
ACTAAAGACA TCACTGACAC ATCAATTGGG GCTTATTGGA CATCTGCTCC AGGAATGGTT 
CGCGGTTACA GGGTCTCATG GAAATCACTT TATGATGATG TTGACACTGG AGAGAAAAAT 
CTGCCTGAAG ATGCAATTCA TACGATGATA GAAAATCTGC AGCCAGAGAC CAAATACAGA 
ATTTCAGTAT TTGCCACTTA CAGCAGTGGA GAAGGAGAAC CTTTGACTGG AGATGCCACA 
ACTGAATTAT CTCAAGATTC CAAAACCCTG AAAGTAGATG AAGAAACAGA AAACACAATG 
AGAGTTACAT GGAAACCAGC ACCAGGGAAA GTCGTCAACT ACCGTGTTGT CTATCGCCCT 
CATGGGAGAG GGAAGCAAAT GGTTGCTAAG GTGCCCCCCA CAGTCACTTC GACAGTGTTA 
AAGCGACTTC AGCCACAGAC CACATATGAC ATCACAGTTC TTCCTATTTA CAAGATGGGA 
GAAGGAAAGC TTAGGCAAGG ATCAGGAACA ACAGCTTCTC GGTTTAAGTC TCCTAGAAAC 
CTCAAAACAT CTGACCCAAC CATGTCAAGC TTCCGAGTGA CTTGGGAGCC TGCCCCTGGG 
GAAGTGAAGG GTTATAAAGT CACATTCCAC CCTACGGGGG ATGACAGAAG ACTGGGGGAG 
TTAGTGGTTG GACCCTATGA CAACACAGTT GTTTTGGAGG AACTTAGGGC TGGTACCACC 
TATAAAGTAA ATGTTTTTGG AATGTTTGAT GGAGGAGAAA GCTCACCACT TGTTGGACAA 
GAAATGACAA CCCTTTCCGA CACAACTGTT ATGCCAATTT TATCTTCTGG GATGGAGTGT 
CTCACCAGAG CTGAGGCAGA CATTGTGTTG CTGGTGGATG GATCATGGAG CATCGGCCGG 
GCAAATTTTA GAACCGTGAG GAGTTTCATT TCTCGTATTG TGGAAGTCTT TGACATTGGC 
CCCAAAAGAG TACAAATTGC TCTTGCTCAG TATAGTGGGG ATCCCAGAAC AGAGTGGCAG 
TTAAATGCAC ACAGAGACAA GAAGAGCTTG TTGCAAGCTG TGGCAAACTT GCCGTACAAA 
GGAGGCAATA CTCTCACAGG CATGGCTTTG AATTTCATTC GCCAACAGAA CTTCAGGACC 
CAAGCTGGCA TGAGACCTCG AGCTCGAAAA ATTGGTGTGC TCATTACTGA TGGAAAATCA 
CAAGACGATG TTGAAGCACC TTCAAAGAAA CTCAAGGATG AGGGAGTGGA GCTGTTTGCT 
ATTGGTATTA AAAATGCTGA TGAAGTCGAA TTAAAGATGA TTGCAACTGA TCCTGATGAT 
ACCCATGCAT ACAATGTGGC AGATTTTGAG TCACTCTCCA GGATAGTGGA TGATCTCACC 
ATTAATTTGT GTAACAGTGT CAAAGGTCCA GGTGATTTGG AAGCACCTTC TAACTTAGTT 
ATTTCTGAGC GAACCCATCG TTCTTTTAGA GTGAGCTGGA CACCACCTTC TGACAGTGTG 
GATCGATATA AGGTGGAATA CTATCCAGTT TCTGGAGGGA AACGTCAAGA ATTTTATGTG 
AGTCGAATGG AAACTAGCAC AGTGCTGAAA GATCTGAAAC CTGAAACTGA ATATGTTGTC 
AATGTGTATT CTGTGGTAGA AGATGAATAT AGTGAGCCTC TGAAGGGGAC AGAAAAAACC 
TTGCCAGTGC CTGTAGTCAG CCTGAATATT TATGATGTTG GCCCTACCAC CATGCATGTG 
CAGTGGCAGC CTGTGGGAGG AGCTACTGGC TACATCTTGT CATACAAACC TGTTAAGGAC 
ACAGAGCCAA CAAGACCCAA AGAGGTGCGT TTGGGGCCAA CAGTGAATGA CATGCAGCTG 
ACTGACCTTG TTCCCAACAC GGAGTATGCA GTCACAGTCC AGGCTGTCCT GCACGACCTC 
ACTAGTGAAC CTGTCACTGT TCGGGAAGTC ACCTTGCCTT TACCCAGACC TCAGGATCTG 
AAACTCAGAG ATGTGACTCA CAGCACTATG AATGTCTTTT GGGAACCTGT GCCTGGAAAA 
GTGCGTAAAT ATATTGTTCG ATACAAAACA CCAGAAGAGG ATGTCAAAGA GGTAGAGGTG 
GACAGATCAG AGACCAGCAC TTCCCTCAAA GACCTCTTCT CACAGACCTT GTACACAGTC 
AGCGTTTCTG CAGTACATGA CGAGGGGGAG TCTCCTCCAG TGACTGCTCA AGAAACTACC 
CGACCCGTGC CAGCCCCAAC AAACTTAAAG ATTACTGAAG TAACATCAGA GGGTTTCAGA 
GGGACTTGGG ATCATGGAGC TTCAGATGTG TCTCTCTACA GAATAACTTG GGCACCTTTT 
GGAAGCTCAG ATAAGATGGA GACCATCTTA AATGGAGATG AAAACACTTT GGTGTTCGAA 
AACCTGAACC CCAACACCAT CTATGAAGTT TCCATTACTG CCATCTATCC TGATGAGTCA 
GAAAGTGATG ACCTGATTGG CAGTGAGCGC ACACTGCCTA TCTTAACAAC ACAAGCTCCC 
AAAAGTGGCC CACGAAACCT TCAAGTGTAC AATGCAACAT CTAACAGCCT GACTGTTAAG 
TGGGATCCTG CTAGTGGTCG TGTGCAGAAA TATAGGATCA CTTATCAGCC TTCCACAGGG 
GAAGGCAATG AGCAAACGAC CACAATAGGA GGACGGCAGA ACAGTGTGGT CCTGCAGAAA 
CTGAAGCCAG ACACTCCTTA CACTATCACC GTATCCTCTC TGTATCCTGA TGGTGAAGGA 
GGTCGGATGA CGGGAAGAGG CAAGACCAAA CCTCTAAACA CTGTAAGGAA CCTGAGAGTG 
TATGACCCTT CTACCAGCAC CTTGAATGTC CGCTGGGACC ATGCAGAGGG AAATCCTCGT 
CAGTACAAGC TCTTCTATGC ACCAGCAGCA GGTGGTCCAG AGGAACTGGT ACCAATCCCC 
GGGAATACCA ATTATGCCAT TCTTAGGAAT CTGCAGCCAG ATACCTCATA CACTGTGACT 
GTAGTTCCCG TTTATACTGA AGGTGATGGG GGACGCACAT CAGATACTGG AAGGACATTG 
ATGAGAGGAC TGGCAAGAAA TGTCCAAGTA TACAATCCTA CACCTAACAG CCTCGATGTT 
CGCTGGGACC CTGCTCCAGG ACCTGTGCTG CAATATCGCG TTGTGTATTC TCCTGTGGAT 
GGCACAAGAC CCTCAGAATC TATAGTAGTG CCAGGAAACA CGCGCATGGT GCATCTGGAG 
CGGCTGATTC CGGACACACT CTATTCCGTG AACCTTGTGG CTCTGTACTC GGATGGAGAG 
GGAAATCCCA GCCCTGCCCA GGGCCGAACG CTACCACGCA GTGGACCAAG GAACCTGAGA 
GTCTTTGGTG AAACAACCAA TAGCCTCTCG GTAGCCTGGG ATCATGCTGA TGGGCCAGTT 
CAGCAGTACA GGATCATCTA TTCTCCCACT GTTGGTGATC CAATTGATGA ATATACCACA 
GTCCCAGGCA GAAGAAACAA TGTAATACTG CAGCCCCTGC AACCTGACAC TCCATATAAA 
ATTACTGTTA TTGCTGTTTA TGAAGATGGA GATGGTGGCC ATCTAACAGG AAATGGAAGA 
ACTGTGGGAC TCCTTCCTCC TCAGAACATA CACATCTCTG ACGAATGGTA TACAAGATTC 
AGGGTGTCCT GGGATCCTTC ACCTTCTCCA GTTCTTGGAT ATAAAATAGT ATATAAGCCA 
GTGGGTTCCA ATGAGCCCAT GGAAGCCTTT GTTGGAGAAA TGACATCATA TACCTTACAC 
AATCTCAATC CCAGCACCAC CTACGATGTG AATGTTTATG CTCAATATGA TTCTGGACTC 
AGTGTCCCCT TGACAGATCA AGGCACTACA TTATATTTAA ATGTAACAGA TCTGAAAACT 
TACCAGATTG GGTGGGATAC ATTCTGTGTC AAATGGTCAC CTCACCGGGC AGCCACCTCC 
TACAGGCTAA AACTAAGCCC TGCAGATGGA ACAAGGGGAC AAGAAATTAC AGTGCGTGGA 
TCAGAAACCA GTCACTGCTT CACTGGCCTT TCACCAGACA CTGATTATGG TGTCACTGTT 
TTTGTGCAGA CACCAAATCT CGAGGGACCA GGAGTCTCTG TTAAAGAACA TACCACTGTG 
AAACCAACAG AAGCCCCTAC AGAGCCACCC ACACCTCCTC CCCCTCCCAC CATTCCACCA 
GCCCGGGATG TATGCAAAGG GGCCAAGGCA GATATTGTGT TCTTGACTGA TGCCTCCTGG 
AGCATTGGGG ACGATAATTT TAACAAAGTT GTAAAATTCA TCTTCAATAC TGTGGGAGGC 
TTTGATGAAA TCAGTCCTGC TGGGATTCAG GTTTCATTTG TGCAATACAG CGATGAGGTC 
AAGTCTGAGT TCAAGCTGAA CACGTACAAT GACAAGGCCC TAGCCCTTGG GGCCCTCCAG 
AATATTAGGT ACAGAGGAGG AAACACAAGA ACAGGCAAGG CCCTCACGTT TATCAAGGAG 
AAAGTCTTGA CTTGGGAGAG CGGCATGAGG AAGAATGTCC CTAAGGTGTT GGTTGTGGTC 
ACGGACGGTC GGTCCCAGGA TGAGGTCAAG AAGGCGGCTT TGGTCATCCA GCAGTCAGGG 
TTCAGTGTCT TTGTAGTTGG TGTGGCTGAT GTCGACTACA ATGAGCTTGC CAACATTGCC 
AGCAAACCAA GTGAACGGCA CGTGTTCATT GTGGACGACT TTGAATCTTT TGAGAAGATC 
GAAGACAATC TTATTACATT TGTTTGTGAA ACTGCCACTT CAAGTTGTCC TCTCATTTAT 
TTGGATGGCT ACACCTCACC AGGTTTTAAA ATGCTTGAAG CATACAACCT GACAGAAAAG 
AATTTTGCTT CTGTACAAGG AGTATCTTTG GAGTCAGGGT CTTTCCCCAG CTACTCAGCA 
TACAGGATTC AGAAGAATGC GTTTGTGAAT CAGCCTACAG CAGACCTACA CCCAAATGGA 
CTCCCTCCTT CATACACGAT TATATTATTA TTCAGACTTC TCCCAGAAAC TCCCAGTGAC 
CCTTTTGCAA TTTGGCAAAT CACAGACAGA GACTACAAAC CACAAGTTGG AGTGATTGCA 
GATCCTTCTA GCAAGACGTT ATCATTCTTT AACAAGGATA CAAGAGGCGA GGTGCAAACT 
GTTACATTTG ACACAGAAGA AGTAAAGACA TTATTTTATG GAAGTTTTCA CAAGGTTCAT 
ATTGTAGTGA CCTCAAAAAG TGTTAAGATT TACATTGACT GCTATGAAAT TATAGAAAAA 
GACATCAAGG AAGCTGGAAA TATAACAACT GATGGTTATG AAATTCTTGG AAAACTCCTT 
AAAGGGGAAA GGAAATCAGC CGCATTCCAA ATCCAGAGTT TTGACATTGT CTGCAGTCCA 
GTGTGGACCA GTAGAGACAG ATGCTGTGAT ATTCCCTCTA GGAGAGATGA GGGAAAATGC 
CCTGCTTTTC CAAATTCTTG CACATGTACA CAGGACAGCG TTGGACCTCC AGGACCTCCA 
GGCCCTGCAG GAGGACCTGG TGCTAAAGGT CCCAGAGGTG AAAGAGGTAT CAGTGGGGCA 
ATTGGGCCCC CTGGTCCTCG TGGAGACATA GGTCCTCCAG GCCCCCAGGG TCCTCCAGGC 
CCTCAGGGAC CCAATGGACT CTCTATTCCG GGAGAGCAAG GTCGCCAAGG GATGAAAGGT 
GATGCTGGAG AGCCAGGACT TCCAGGCCGA ACAGGAACCC CAGGATTACC TGGCCCACCA 
GGACCAATGG GACCTCCAGG AGACAGAGGC TTCACTGGAA AAGACGGTGC AATGGGACCC 
AGGGGCCCAC CAGGGCCGCC GGGAAGCCCA GGCTCCCCAG GAGTCACAGG ACCAAGTGGG 
AAGCCAGGAA AACCTGGAGA TCATGGCAGA CCAGGTCCAT CTGGGTTGAA AGGAGAAAAA 
GGTGATAGGG GAGACATTGC TTCCCAGAAC ATGATGCGAG CAGTTGCAAG ACAAGTCTGT 
GAACAATTGA TAAGTGGTCA GATGAACAGA TTCAATCAGA TGCTGAATCA GATTCCAAAT 
GATTACCAGT CCAGTCGCAA CCAGCCAGGC CCGCCGGGTC CACCGGGACC TCCTGGTAGC 
GCAGGAGCCA GAGGAGAACC TGGGCCTGGG GGGCGGCCAG GCTTCCCGGG CACACCAGGG 
ATGCAGGGAC CCCCTGGGGA ACGAGGTTTG CCAGGAGAGA AAGGTGAAAG GGGTACTGGA 
TCTTCAGGAC CTCGGGGGCT GCCTGGCCCC CCAGGTCCAC AAGGAGAATC CAGAACAGGT 
CCACCAGGGT CCACAGGTTC AAGAGGTCCC CCTGGCCCCC CTGGCCGTCC TGGAAACTCA 
GGTATCCGAG GACCCCCAGG TCCTCCTGGA TACTGTGATT CTTCTCAGTG TGCCAGCATC 
CCATACAACG GGCAAGGCTA TCCAGAGCCT TACGTGCCCG AGGGCGGGGC CTACCTGCCT 
GAGCGCGAGC CCTTCATCGT GCCGGTGGAG CCCGAGCGGA CAGCGGAATA CGAGGACTAC 
GGCGCAGACG AGCCTGCAGA GGAGCCTCCT GAGCCCCACC GGCGCTGGCG GCGCGCCCTG 
CCCCACGGAC CCGGGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	COL12A1
Entrez Gene ID	1303
Full Name	collagen type XII alpha 1 chain
Synonyms	BA209D8.1,BTHLM2,COL12A1L,DJ234P15.1,UCMD2
General protein information	Preferred Names collagen type XII alpha 1 chain Names collagen alpha-1(XII) chain collagen type XII proteoglycan collagen, type XII, alpha 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q13-q14.1
Summary	This gene encodes the alpha chain of type XII collagen, a member of the FACIT (fibril-associated collagens with interrupted triple helices) collagen family. Type XII collagen is a homotrimer found in association with type I collagen, an association that is thought to modify the interactions between collagen I fibrils and the surrounding matrix. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008].
Disorder MIM:	120320
Disorder Html:	?Ullrich congenital muscular dystrophy 2, 616470 (3); Bethlem myopathy 2, 616471 (3)

Rattus norvegicus (Norway rat)	Col12a1	XP_006243503.1
Danio rerio (zebrafish)	col12a1a	XP_002665305.3
Canis lupus familiaris (dog)	COL12A1	XP_005627591.1
Gallus gallus (chicken)	COL12A1	NP_990352.1
Homo sapiens (human)	COL12A1	NP_004361.3
Pan troglodytes (chimpanzee)	COL12A1	XP_001142912.1
Xenopus tropicalis (tropical clawed frog)	col12a1	XP_002936246.2
	COL12A1	XP_001109727.2
Bos taurus (cattle)	COL12A1	NP_001193426.1
Mus musculus (house mouse)	Col12a1	NP_031756.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

COL12A1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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