DLST cDNA ORF clone, Haliaeetus leucocephalus(bald eagle)

Gene
Clones
gRNAs

Summary

Gene Symbol	DLST
Entrez Gene ID	104841808
Full Name	dihydrolipoamide S-succinyltransferase
Gene Type	protein-coding
Organism	Haliaeetus leucocephalus(bald eagle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010583715.1	XP_010582017.1	dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial

Pathways from BioSystems
Gene Ontology

The following DLST gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DLST cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHa124390	XM_010583715.1 Latest version!	Haliaeetus leucocephalus dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex) (DLST), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DLST ( XM_010583715.1 ) cDNA ORF clone, Haliaeetus leucocephalus(bald eagle) -> XP_010582017.1 Haliaeetus leucocephalus dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex) (DLST), mRNA.

Price & Availability↑

CloneID	OHa124390
Clone ID Related Accession (Same CDS sequence)	XM_010583715.1
Accession Version	XM_010583715.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-05
Organism	Haliaeetus leucocephalus(bald eagle)
Product	dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010973368.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Haliaeetus leucocephalus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTG ATTTTAGCCA TTGTGTGAAT GGGAACTGTA CTCTGGCAAG ATGCTCTCTG 
TCTGGTGTGG CTGGGAGCCA GGGACTGGCT TACATGAACA GCAGGAAGCT TGTAGTAAAT 
AGCTCCAGTG TCTTCACCGT CCGTTACTTC AGAACCACTG CAGTACGTAG GGATGACGTG 
GTTACGGTGA ACACACCAGC CTTTGCAGAG TCAGTCACAG AAGGAGATGT CAGGTGGGAG 
AAAGCTGTTG GAGACACAGT GGCGGAAGAT GAAGTGGTGT GTGAGATTGA AACAGACAAG 
ACATCAGTGC AAGTTCCAGC CCCAGCAGCT GGTGTGATTG AAGCCCTTCT GGTACCTGAT 
GGTGGCAAAG TGGAAGGGGG AACGCCTCTG TTCAAACTCA GGAAAACTGG GGCTGCTCCT 
GCCAAGGCCA AACCAGCAGC AGCCCCTCCT CCTCCTGCAG CCCCTGAACC TGTAGCTGCA 
GCTGCTCCTC CCCCTGCTGC AGCACCAATT CCCACTACAA TGCCACCAGT GCCGCCTGTG 
TCAACTCAGC CCATTGATAG CAAACCAGTG TCTGCGGTGA AGCCAGCTGC AGCCCCAGCG 
GCAGCCCCTC CTGGGGAGGC AGTGCCCAGC AAAGGTGCCC GATCAGAACA TAGGGTGAAA 
ATGAATAGGA TGCGTCAGCG TATTGCTCAG CGGCTGAAAG AGGCTCAGAA TACTTGTGCC 
ATGCTGACCA CTTTCAATGA GATCGATATG AGCAACATCC GGGAGATGAG AGCTGTACAC 
AAGGATCCCT TCCTGAAAAA GCACAATCTG AAGCTAGGTT TCATGTCAGC TTTTGTGAAA 
GCTGCAGCTT TTGCTCTGCA GGACCAGCCC GTTGTGAATG CAGTGATTGA TGACACGACC 
AAAGAGATTG TGTACAGGGA CTATGTGGAC ATCAGTGTTG CTGTAGCAAC TCCCCGGGGT 
CTTGTGGTCC CTGTCGTTAG GAATGTAGAA AACATGAACT TTGCTGACAT AGAGCGTGCT 
ATCTACGAGT TGGGGGAGAA GGCACGGAAG AACGAACTGG CCATTGAAGA CATGGATGGC 
GGCACTTTCA CAATCAGCAA CGGAGGGGTT TTTGGGTCAC TCTTTGGGAC ACCTATCATT 
AACCCACCCC AGTCTGCCAT CTTAGGCATG CATGCCATCT TTGACAGGCC TGTAGCTGTG 
GGAGGCAAGA TCGAGGTGCG GCCCATGATG TATGTGGCGC TGACGTATGA TCACCGGCTG 
ATTGATGGCA GAGAGGCAGT GACTTTCCTG CGCAAGATCA AGGCAGCGGT GGAGGATCCC 
CGCGTGCTGC TGCTTGACCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010582017.1
CDS	542..1885
Translation	MRFDFSHCVNGNCTLARCSLSGVAGSQGLAYMNSRKLVVNSSSVFTVRYFRTTAVRRDDVVTVNTPAFAESVTEGDVRWEKAVGDTVAEDEVVCEIETDKTSVQVPAPAAGVIEALLVPDGGKVEGGTPLFKLRKTGAAPAKAKPAAAPPPPAAPEPVAAAAPPPAAAPIPTTMPPVPPVSTQPIDSKPVSAVKPAAAPAAAPPGEAVPSKGARSEHRVKMNRMRQRIAQRLKEAQNTCAMLTTFNEIDMSNIREMRAVHKDPFLKKHNLKLGFMSAFVKAAAFALQDQPVVNAVIDDTTKEIVYRDYVDISVAVATPRGLVVPVVRNVENMNFADIERAIYELGEKARKNELAIEDMDGGTFTISNGGVFGSLFGTPIINPPQSAILGMHAIFDRPVAVGGKIEVRPMMYVALTYDHRLIDGREAVTFLRKIKAAVEDPRVLLLDL

Target ORF information:

RefSeq Version	XM_010583715.1
Organism	Haliaeetus leucocephalus(bald eagle)
Definition	Haliaeetus leucocephalus dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex) (DLST), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010583715.1

ORF Insert Sequence:

ATGAGATTTG ATTTTAGCCA TTGTGTGAAT GGGAACTGTA CTCTGGCAAG ATGCTCTCTG 
TCTGGTGTGG CTGGGAGCCA GGGACTGGCT TACATGAACA GCAGGAAGCT TGTAGTAAAT 
AGCTCCAGTG TCTTCACCGT CCGTTACTTC AGAACCACTG CAGTACGTAG GGATGACGTG 
GTTACGGTGA ACACACCAGC CTTTGCAGAG TCAGTCACAG AAGGAGATGT CAGGTGGGAG 
AAAGCTGTTG GAGACACAGT GGCGGAAGAT GAAGTGGTGT GTGAGATTGA AACAGACAAG 
ACATCAGTGC AAGTTCCAGC CCCAGCAGCT GGTGTGATTG AAGCCCTTCT GGTACCTGAT 
GGTGGCAAAG TGGAAGGGGG AACGCCTCTG TTCAAACTCA GGAAAACTGG GGCTGCTCCT 
GCCAAGGCCA AACCAGCAGC AGCCCCTCCT CCTCCTGCAG CCCCTGAACC TGTAGCTGCA 
GCTGCTCCTC CCCCTGCTGC AGCACCAATT CCCACTACAA TGCCACCAGT GCCGCCTGTG 
TCAACTCAGC CCATTGATAG CAAACCAGTG TCTGCGGTGA AGCCAGCTGC AGCCCCAGCG 
GCAGCCCCTC CTGGGGAGGC AGTGCCCAGC AAAGGTGCCC GATCAGAACA TAGGGTGAAA 
ATGAATAGGA TGCGTCAGCG TATTGCTCAG CGGCTGAAAG AGGCTCAGAA TACTTGTGCC 
ATGCTGACCA CTTTCAATGA GATCGATATG AGCAACATCC GGGAGATGAG AGCTGTACAC 
AAGGATCCCT TCCTGAAAAA GCACAATCTG AAGCTAGGTT TCATGTCAGC TTTTGTGAAA 
GCTGCAGCTT TTGCTCTGCA GGACCAGCCC GTTGTGAATG CAGTGATTGA TGACACGACC 
AAAGAGATTG TGTACAGGGA CTATGTGGAC ATCAGTGTTG CTGTAGCAAC TCCCCGGGGT 
CTTGTGGTCC CTGTCGTTAG GAATGTAGAA AACATGAACT TTGCTGACAT AGAGCGTGCT 
ATCTACGAGT TGGGGGAGAA GGCACGGAAG AACGAACTGG CCATTGAAGA CATGGATGGC 
GGCACTTTCA CAATCAGCAA CGGAGGGGTT TTTGGGTCAC TCTTTGGGAC ACCTATCATT 
AACCCACCCC AGTCTGCCAT CTTAGGCATG CATGCCATCT TTGACAGGCC TGTAGCTGTG 
GGAGGCAAGA TCGAGGTGCG GCCCATGATG TATGTGGCGC TGACGTATGA TCACCGGCTG 
ATTGATGGCA GAGAGGCAGT GACTTTCCTG CGCAAGATCA AGGCAGCGGT GGAGGATCCC 
CGCGTGCTGC TGCTTGACCT GTAG