DACT1 cDNA ORF clone, Gekko japonicus

Gene
Clones
gRNAs

Summary

Gene Symbol	DACT1
Entrez Gene ID	107120639
Full Name	dishevelled binding antagonist of beta catenin 1
Gene Type	protein-coding
Organism	Gekko japonicus

mRNA and Protein(s)

mRNA	Protein	Name
XM_015423373.1	XP_015278859.1	dapper homolog 1

Pathways from BioSystems
Gene Ontology

The following DACT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DACT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGe31909	XM_015423373.1 Latest version!	Gekko japonicus dishevelled-binding antagonist of beta-catenin 1 (DACT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DACT1 ( XM_015423373.1 ) cDNA ORF clone, Gekko japonicus -> XP_015278859.1 Gekko japonicus dishevelled-binding antagonist of beta-catenin 1 (DACT1), mRNA.

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CloneID	OGe31909
Clone ID Related Accession (Same CDS sequence)	XM_015423373.1
Accession Version	XM_015423373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-19
Organism	Gekko japonicus
Product	dapper homolog 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015173723.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gekko japonicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 5% of CDS bases ##RefSeq-Attributes-END##

ATGGCGTCGA GCGGCAGAAT CCGGGCGGAG ACGGAATTGT GGAGGTGCGG TGACATGGCG 
GCGCAGCAGA GCCGGTATAA CATGAGCCAG AGCTGCGGCT GGCCTCGCCG TCGGAACTGC 
TTGAGAAGGA GGGACGCCGG CTTGTTGAAC CAGCTCCAAG AACTGGACAA GCAAATCAGT 
GATCTGAGGC TGGACGTTGA AAAAACAGCA GACGAACGGC TGGAGATGGA CAGTCGACCC 
AGCTCAGGGT TTTACGAGCT AAGTGATGGC GGGGCTTCTG GGTCCCTCTC CAACTCGTCC 
AACTCCGTTT TCAGCGAGTG TTTATCCAGC TGTCCCTCCA GCGCCTGCTT CTGCAGCCCT 
TTGGATACAT CCTTGAGCAG TTCAGATGGC CGTCCCAAAT CGGCAGATCT TATTGGGTGG 
ATGGAATACA ACAAGGGAGG TCAACCTGAA GACCAGTCAG TGGGGACTGT CTCTCATTCT 
CTGTCCACGC GACATTCGAA CTCACTGGAC GTGATCGCAG AAGTGCACCC AAAATATCAG 
TGTGACCTGG TGTCCAAAAA TGGGAATGAC GTGTACCGGT ACCCCAGTCC CTTACATGCG 
GTTGCAGTCC AGAGCCCCAT GTTTCTGTTG CCAGCAACGA GTAGCCCCCC AAGAGAAGAG 
GAAGACAGGA GTATTTGCAA CGTTGGTGAG GTCTGTGTTG GATCGGAACT CGATACAAAA 
ACCGGCACTT CCTGTCACCC GTCAGAGTCT CATTCCGCAT CCCTTCCCAT GGCCACCAAA 
AAAATGGATG GATACATCTT GAGTCTCGTG CAGAAAAAGA TCCCGTCAGT GAGGACTAAC 
AAACCGAGAA CGAATCTGAA CGCAGACCCT GCCAAAGGAA TCTTGAGACA CGGAAGCATT 
TGTGCTCGGC AGGGGGCCAG TGCGGCCCAT ACCAATGCAG TCCTTGTTAA GAATCCTAGC 
CAGGTGTTTC CCCGTTCCGC GACAGCCACC CCCGTGTTGG ATAATGGCGC CTTTTCTCCC 
TCCAAGCAGC GTTTGCGAGA AGCAAGCATT GAGAATCTGG AGACCAAAAA GATTCCTTTA 
CCCACAGCCT TTTCACCAAA TCCTAACCAT GAACTTCAGA GCAAACCCCC CTCATCCAGG 
ACCGTCAAAC CTCAAGAGGT TATACGGTGT CCTGTGGTTA GCAAGGGTGA TGGGCCCAAG 
GAAAGAGGCC AAACCTTTGT GACTAAAGAA AGCCCTTGCC GGAACACGCC ACCTTTGAAG 
GAAAACAAAG CCATCCAGAT ACCCAAAAAG ATACCGTTAA AAAACAATGG GGGACAAGCA 
ACTCACTCTG TTTCGCCTCC TCATGAGACC AGGCCATCTT TGGATTTCAA AAGTGAGGGA 
TCATCCTCGC AAAGCCAGGA GGAAGGGTTA CTGGTGAACG CCCAGTATGT ACCAGCCCAA 
CCACAAACAT TCAGACTTCA CAAAGGCACC AGGAGTGTCA AAATTCTAAA AAGTTCCATG 
GTGAAACACA GGTCCCATCT CGTCACGGTC CTGGAAAACA GTCCCCTGGC TGGGCGGGAG 
AAAGGCAAAC CCCCTGGCAA AAAATGCCGT TTCCCCGATG ACCTGGATAC AAATAAGAAG 
CTGAAAAAGA CAACCTCAAG GGGAAAGAAA AGTGGCCATT TGCCAACGGA GACAAGCCTT 
TTGAGCAGGC CCCCTGGTGT CCTTAGATCC ACAGCGAGAC CCCACGGGTA CAGCAGAGAA 
CCGGTTGTAG CCAAACCTAA GCACAAGCGA GCTGACTATC GCCGCTGGAA ACTGTCAGCG 
GAGGTGTCTT ATGAAGAAGC CCTCAAGAGG GCCAGAAGAA ACCGGAGAGA AGGCCTTGGG 
GTCTACTCAC AAGTTACCCA TCCCTACGCT AGCCCCTACG CCTATGTTGC TAGTGACTCG 
GAATATTCGG CTGAGTGCGA ATCTCTTTTC CATTCCACTG TAGTAGATAC CAGCGAGGAC 
GAAAGGAGTA ATTACACCAC GAACTGCTTT GGGGATAGTG AGTCAAGCTT GAGCGAGGTC 
GAGTTTGTCG GCGAAAGCAC GACGACCAGT GACTCGGACG AGAGTGGGGG CCTCATCTGG 
TCCCAGTTCG TCCAAACTCT CCCCATCCAG ACAGTCGCCG CTGCTTCGGA GCTCCACGAT 
AACGCGGCCA AAACTTTTGT GAAGATTAAG GCCTCTCATA ACCTGAAGAA GAAAATCCTC 
CGCTTTCGGT CCGGCTCTCT CAAACTCATG ACTACTGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015278859.1
CDS	1..2262
Translation	MASSGRIRAETELWRCGDMAAQQSRYNMSQSCGWPRRRNCLRRRDAGLLNQLQELDKQISDLRLDVEKTADERLEMDSRPSSGFYELSDGGASGSLSNSSNSVFSECLSSCPSSACFCSPLDTSLSSSDGRPKSADLIGWMEYNKGGQPEDQSVGTVSHSLSTRHSNSLDVIAEVHPKYQCDLVSKNGNDVYRYPSPLHAVAVQSPMFLLPATSSPPREEEDRSICNVGEVCVGSELDTKTGTSCHPSESHSASLPMATKKMDGYILSLVQKKIPSVRTNKPRTNLNADPAKGILRHGSICARQGASAAHTNAVLVKNPSQVFPRSATATPVLDNGAFSPSKQRLREASIENLETKKIPLPTAFSPNPNHELQSKPPSSRTVKPQEVIRCPVVSKGDGPKERGQTFVTKESPCRNTPPLKENKAIQIPKKIPLKNNGGQATHSVSPPHETRPSLDFKSEGSSSQSQEEGLLVNAQYVPAQPQTFRLHKGTRSVKILKSSMVKHRSHLVTVLENSPLAGREKGKPPGKKCRFPDDLDTNKKLKKTTSRGKKSGHLPTETSLLSRPPGVLRSTARPHGYSREPVVAKPKHKRADYRRWKLSAEVSYEEALKRARRNRREGLGVYSQVTHPYASPYAYVASDSEYSAECESLFHSTVVDTSEDERSNYTTNCFGDSESSLSEVEFVGESTTTSDSDESGGLIWSQFVQTLPIQTVAAASELHDNAAKTFVKIKASHNLKKKILRFRSGSLKLMTTV

Target ORF information:

RefSeq Version	XM_015423373.1
Organism	Gekko japonicus
Definition	Gekko japonicus dishevelled-binding antagonist of beta-catenin 1 (DACT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015423373.1

ORF Insert Sequence:

ATGGCGTCGA GCGGCAGAAT CCGGGCGGAG ACGGAATTGT GGAGGTGCGG TGACATGGCG 
GCGCAGCAGA GCCGGTATAA CATGAGCCAG AGCTGCGGCT GGCCTCGCCG TCGGAACTGC 
TTGAGAAGGA GGGACGCCGG CTTGTTGAAC CAGCTCCAAG AACTGGACAA GCAAATCAGT 
GATCTGAGGC TGGACGTTGA AAAAACAGCA GACGAACGGC TGGAGATGGA CAGTCGACCC 
AGCTCAGGGT TTTACGAGCT AAGTGATGGC GGGGCTTCTG GGTCCCTCTC CAACTCGTCC 
AACTCCGTTT TCAGCGAGTG TTTATCCAGC TGTCCCTCCA GCGCCTGCTT CTGCAGCCCT 
TTGGATACAT CCTTGAGCAG TTCAGATGGC CGTCCCAAAT CGGCAGATCT TATTGGGTGG 
ATGGAATACA ACAAGGGAGG TCAACCTGAA GACCAGTCAG TGGGGACTGT CTCTCATTCT 
CTGTCCACGC GACATTCGAA CTCACTGGAC GTGATCGCAG AAGTGCACCC AAAATATCAG 
TGTGACCTGG TGTCCAAAAA TGGGAATGAC GTGTACCGGT ACCCCAGTCC CTTACATGCG 
GTTGCAGTCC AGAGCCCCAT GTTTCTGTTG CCAGCAACGA GTAGCCCCCC AAGAGAAGAG 
GAAGACAGGA GTATTTGCAA CGTTGGTGAG GTCTGTGTTG GATCGGAACT CGATACAAAA 
ACCGGCACTT CCTGTCACCC GTCAGAGTCT CATTCCGCAT CCCTTCCCAT GGCCACCAAA 
AAAATGGATG GATACATCTT GAGTCTCGTG CAGAAAAAGA TCCCGTCAGT GAGGACTAAC 
AAACCGAGAA CGAATCTGAA CGCAGACCCT GCCAAAGGAA TCTTGAGACA CGGAAGCATT 
TGTGCTCGGC AGGGGGCCAG TGCGGCCCAT ACCAATGCAG TCCTTGTTAA GAATCCTAGC 
CAGGTGTTTC CCCGTTCCGC GACAGCCACC CCCGTGTTGG ATAATGGCGC CTTTTCTCCC 
TCCAAGCAGC GTTTGCGAGA AGCAAGCATT GAGAATCTGG AGACCAAAAA GATTCCTTTA 
CCCACAGCCT TTTCACCAAA TCCTAACCAT GAACTTCAGA GCAAACCCCC CTCATCCAGG 
ACCGTCAAAC CTCAAGAGGT TATACGGTGT CCTGTGGTTA GCAAGGGTGA TGGGCCCAAG 
GAAAGAGGCC AAACCTTTGT GACTAAAGAA AGCCCTTGCC GGAACACGCC ACCTTTGAAG 
GAAAACAAAG CCATCCAGAT ACCCAAAAAG ATACCGTTAA AAAACAATGG GGGACAAGCA 
ACTCACTCTG TTTCGCCTCC TCATGAGACC AGGCCATCTT TGGATTTCAA AAGTGAGGGA 
TCATCCTCGC AAAGCCAGGA GGAAGGGTTA CTGGTGAACG CCCAGTATGT ACCAGCCCAA 
CCACAAACAT TCAGACTTCA CAAAGGCACC AGGAGTGTCA AAATTCTAAA AAGTTCCATG 
GTGAAACACA GGTCCCATCT CGTCACGGTC CTGGAAAACA GTCCCCTGGC TGGGCGGGAG 
AAAGGCAAAC CCCCTGGCAA AAAATGCCGT TTCCCCGATG ACCTGGATAC AAATAAGAAG 
CTGAAAAAGA CAACCTCAAG GGGAAAGAAA AGTGGCCATT TGCCAACGGA GACAAGCCTT 
TTGAGCAGGC CCCCTGGTGT CCTTAGATCC ACAGCGAGAC CCCACGGGTA CAGCAGAGAA 
CCGGTTGTAG CCAAACCTAA GCACAAGCGA GCTGACTATC GCCGCTGGAA ACTGTCAGCG 
GAGGTGTCTT ATGAAGAAGC CCTCAAGAGG GCCAGAAGAA ACCGGAGAGA AGGCCTTGGG 
GTCTACTCAC AAGTTACCCA TCCCTACGCT AGCCCCTACG CCTATGTTGC TAGTGACTCG 
GAATATTCGG CTGAGTGCGA ATCTCTTTTC CATTCCACTG TAGTAGATAC CAGCGAGGAC 
GAAAGGAGTA ATTACACCAC GAACTGCTTT GGGGATAGTG AGTCAAGCTT GAGCGAGGTC 
GAGTTTGTCG GCGAAAGCAC GACGACCAGT GACTCGGACG AGAGTGGGGG CCTCATCTGG 
TCCCAGTTCG TCCAAACTCT CCCCATCCAG ACAGTCGCCG CTGCTTCGGA GCTCCACGAT 
AACGCGGCCA AAACTTTTGT GAAGATTAAG GCCTCTCATA ACCTGAAGAA GAAAATCCTC 
CGCTTTCGGT CCGGCTCTCT CAAACTCATG ACTACTGTCT AA