EVC2 gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_015285796.1	XP_015141282.1	limbin isoform X4
XM_025149755.1	XP_025005523.1	limbin isoform X2
XM_015285793.1	XP_015141279.1	limbin isoform X1
XM_015285796.2	XP_015141282.1	limbin isoform X4
XM_015285795.1	XP_015141281.1	limbin isoform X3
NM_001079749.2	NP_001073217.1	limbin
XM_015285795.2	XP_015141281.1	limbin isoform X3
XM_015285794.1	XP_015141280.1	limbin isoform X2
XM_015285793.2	XP_015141279.1	limbin isoform X1
XM_025149754.1	XP_025005522.1	limbin isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa35734	XM_025149755.1 Latest version!	Gallus gallus EvC ciliary complex subunit 2 (EVC2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa35734	XM_015285794.1 Latest version!	Gallus gallus Ellis van Creveld syndrome 2 (EVC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa35734	XM_025149754.1 Latest version!	Gallus gallus EvC ciliary complex subunit 2 (EVC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa35735	XM_015285795.2 Latest version!	Gallus gallus EvC ciliary complex subunit 2 (EVC2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa24643	NM_001079749.2 Latest version!	Gallus gallus EvC ciliary complex subunit 2 (EVC2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OGa35733	XM_015285793.2 Latest version!	Gallus gallus EvC ciliary complex subunit 2 (EVC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OGa35736	XM_015285796.2 Latest version!	Gallus gallus EvC ciliary complex subunit 2 (EVC2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
View Old Accession Versions >>
OGa35736	XM_015285796.1	Gallus gallus Ellis van Creveld syndrome 2 (EVC2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OGa35735	XM_015285795.1	Gallus gallus Ellis van Creveld syndrome 2 (EVC2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa35733	XM_015285793.1	Gallus gallus Ellis van Creveld syndrome 2 (EVC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
Hide Old Accession Versions >>

EVC2 ( XM_025149755.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025005523.1 Gallus gallus EvC ciliary complex subunit 2 (EVC2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa35734
Clone ID Related Accession (Same CDS sequence)	XM_025149755.1 , XM_015285794.1 , XM_025149754.1
Accession Version	XM_025149755.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	limbin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTGTC TGCGGATGAC AGCACTGCAG TTGCCTGTGG TGGTTCAAGC AAGTGAAGTC 
CCTGATCTGA TCTTGACTTC TGCAGCCTCG GAAGATCAGT GGAGCCATTC AATATTTGCT 
CTGATACCCT CATGGACAAG GAAGATTTTG TTCAAACGAG AGTCTTCTGT TAACCACCAG 
CTGTCTTTTC CCCTTCCACC ACAGCCTGAA GATGTGTCCA ACTTGTCAGC ATCCTCTGGA 
TTTGGAATAA CCCTTCAGAA ATGTGCTGTG GTGGATGTTG AACATGACCC TCAAACTGCA 
TACATTAGAC TTCTGATTAA TAACACCAAC TCACTGCTCT CAAGAAACAT TACTGATCTT 
ATCCTTCTGG ACAATATCAC CGGTCTACAT GTTCAAAAAG ATAGTGGTAA TGAGACAAGA 
GATGGGATGC AAATTTACAG GAAAAGATTC TTGCAAGTTG GGGAATATTT TGCAATCAAC 
TATACTGCAC TTCTTGATGC AAAGAAAACG TGGAAAGGTA GGGTCTTGAC ACTGCCTGCA 
CAGCTGACTT TCAGGAGTTC ATCACAGAAT CAAACAGATC TTGTATCATT GACCACATTG 
TTCACAATAA CTGAAGAAGA AAAGACCGAG GTCTCCTACA GCCATGCCAT CCATGCTTCA 
GGGTTCTTCA TTGCTTTTTT TGTTTCCCTG GTATTGACAT GTGCTGTTTT CTTCATTCTT 
TACCGAACCC AATTATTAAG ATGGAGTTTC TATAGTGAAA ACCAGGAAAT ACAACATGAA 
CTCAGTCAAA ATCACAAACT GGAGCATTCT CAGTTCAGTT CAGGTGACCT CTTCAGTGAA 
GATGTTATTA TGAATGACCA AATCATTGAT ATACTGACCT TTGAAGAGTC GGGGAATATG 
CTTCAGGCTT TAGAAGACTT GGAGGTTGCC AGTCTGACTC GGGCAGATGC TGATTTGGAG 
GCATATCGAA TGCAGATCTG TAAAGAAGTG ATTGCGATAA TGATGAAGAA CATGGTTTTA 
AGTCACAGCC TCATTCCGCA TGTGGAGAAG AGGATGAGTT CAATTTTCAA AAAGCAGTTT 
CTTACAATGG AGAGAGAGTT TCAGGAAGAG TACGAAAGGA AGGTTGTGGC TTTAACAGCT 
GAATGTAATC TGGAAACAAG GAAGAAGATG GAGGCTCAAC GCCAGAAAGA GAGAGCTGCA 
AATGAAGAGG CTGAAGAGTT AATAAGGAAA GTGAATGAAA TGCCTGCTGT AGAATACAAA 
AGTCTTTTGG ATAGACTTCA CAGTCTGGAG CAGGACCATC TGAAGAGGTT CCTTCTGGTA 
AAGCAAGAGG AGTATTTTGC AAAGGCTTAC AGACAACTGG CTGTTTCCCG GAGAAAGGAG 
CTCCATAACA TCTTCTTTAC ACAGATAACA AATGCTACTT TCAAGGGAGA ACTGAAACTG 
GAAATAGCAA AATCATTAGT GGAAGATTAC TCTAAAATAC AGGGAGACAT TGAAGAGCTA 
ATGGATTTTT TGCAAGCCAG CAAGAAGTAT CACCTGAACA GAAGATTTGC TTACAGAGAA 
TATCTTATAA AAAAGATACA GTTAAGGGAT TCTCAGGCCT CTGCTCTTAT AAATGCAGCA 
GCAACTCAGA TATTGAATCT CATTAATAAG ATGGAAAGGG CGGGTCATCT ACATGAAAGT 
CATTTGGGAA TGCTGCTGGA CCGAGCTGAG GCTGAAATTA ATTCTGTTAA ACAAAAATTC 
AATCATGATT TAAAACAAGA AAAGCAAAAG CTTCAGCAGA AACTCATTAC CAAGCGAAAG 
TGGAAAATGT TGCAAAAGAA AGAGCATTGG AAGGAGCAGC TGTCCATAGG GAACCCCTTC 
AAAACCTCAA GGGAGATCAC TGACTACCTG AGCCACTGGA AAACTGTTCT GAGTGATCAT 
GCCATTGAAT TTGAAGAACT TACTGAAAAG CTGGATAATG AAGGCAGTGA AGAGCTGAGA 
GACCTTATAT TTAATCTAGC AGAGAAAACT GTTGAAGAAC TTAAACTTGT TCAATATGGA 
GTATTTGTGC AAGAACTGAT AAAGCTCAGT GTGCCAAAGA TGTTCCTGCT GGAAGTTGTA 
GAGGAGCATA AAAAAGAATT CATTGTTAAA CATGAACACC TTGAAAGGGA AGAACGTGAA 
AAGAGCATGG CTGCTGAAGA GTTGCTTCAG CTCACCAGGC AAAAACTAAG CCAAGAGCTG 
GAAAGTAGTC TTTCACAACA GAAAAAATTA AGGAACTGGG AACAATCAGT ATTCATGCAG 
CTTCTAACTT TGCCCCTTTC ACTGTCTGAA GAGGAATTGC TCAAAATGAG GCAAGAGTTC 
CACTGCTGCT TTTCACAGCT GGACAGCAGT TTGGCTCTGC CCAAGATAAG AGCAAGAGCT 
TTACTTCAGG CTTTTGAGCT GGAGTGGAGG GATGCGCAGC TGCTGAAAGT CAGTCAGAAT 
CTAGCAATGA CCAATAAGCA GCTGCATTCT AAAACGAAAA AAACAGGATC CAAGAATCGA 
AATAAGATTG ATGTTTTGAA GAAATCTTTA CAGGACAAAA TTTTCATTTA TGAAGACTCA 
GTGAAAGTTG AAAACTTGAC TAAGGTCAAT TATGAGTTGC TACTTGAGAA AGACTGTCAG 
ATACATGATC TGGAAAATAA ACTTGGAGAA TATATTGCTT CTTTAGAGTT TCAAAAGAGT 
GTTAAAAAAT GCAAAATGTT GGAGCTCTAT GCAGCAGTCA TAAGTGTGCA AGGTTTGCTC 
TTGGAAAACC TGAGCATGTC AAAAACGCTG TCAAAATCGG AATGTATTCA GATTTTAGAA 
GCTCATAATC CGGAGATTGA AGAACTTGAC AGGAAGCTGG AGTATGAAAT GCTGCATAAA 
GAGTCAGCAC AACAGCAGCA ACATCTAATG AATAAGCAGA GGTGGACACC GGATGGATTG 
AGACCTTCAA GTGAAGTCGT AGAAACAAAT GTAGACAGAC AGGTGACATT TCTGCTAAGA 
CAGGCCATGA ATAGATGTAT GCAGTTTATA AATCTGCACC GGCAAAGCTT GAGAGATGAG 
CAATGGAATT GTATGGTGCT TGAAGATCTC TTGGAAAATA TAGAAATGGA TGCATTTTTG 
GCACTTTATA GTCAGGAGCT CCGACTGGCA GGTTATTTAG CCAGATTGAC AAGGGTACCA 
ATGGGGATGT TGCACAGGAT TCTGAATTTG TTGCTGCCTT CCAGTTCGCA AAGTGAGGTT 
CTTTCTATCC TTGATTCCAT TGGTGTATAT TCAGATGGTG CTGCTGAGAG TGATAGTAAT 
GCAGATGAGT CTGGCAGCTG TAAAAAAAGG AGGAACCAAG AGTGTTGGCA AGCATTGGAG 
TACAAAGTAA GGGAAGATCT GGTAACCAAA AGTTTGGAAA GGATCCTTTC TCTAAATAGG 
AGAAAAGACA GCTTGATAAA GAAGAAGCAA CTTGCCCTTC TGGAAAGACC ATCATTCGTT 
CACCTTGAAA GTTTGCCTAC ACGTCCTCCT GTGGAACGGT CCAGTCCACA TGCTTCTTTT 
AACAGTGTGA CAGCTGAAGC CATCGAGGTA TTAGACACAG GGGAAAAGAT ATTTTTGTTT 
CGTGACCGGA GTGATCCAAC ACTTTCTCTT CATAACTATC CGAGAAAAAA GAAGAAGAAC 
TTTCTTAATT CCAAAAAGGC TGCTCGTGCA AATATCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025005523.1
CDS	1516..5217
Translation	MNCLRMTALQLPVVVQASEVPDLILTSAASEDQWSHSIFALIPSWTRKILFKRESSVNHQLSFPLPPQPEDVSNLSASSGFGITLQKCAVVDVEHDPQTAYIRLLINNTNSLLSRNITDLILLDNITGLHVQKDSGNETRDGMQIYRKRFLQVGEYFAINYTALLDAKKTWKGRVLTLPAQLTFRSSSQNQTDLVSLTTLFTITEEEKTEVSYSHAIHASGFFIAFFVSLVLTCAVFFILYRTQLLRWSFYSENQEIQHELSQNHKLEHSQFSSGDLFSEDVIMNDQIIDILTFEESGNMLQALEDLEVASLTRADADLEAYRMQICKEVIAIMMKNMVLSHSLIPHVEKRMSSIFKKQFLTMEREFQEEYERKVVALTAECNLETRKKMEAQRQKERAANEEAEELIRKVNEMPAVEYKSLLDRLHSLEQDHLKRFLLVKQEEYFAKAYRQLAVSRRKELHNIFFTQITNATFKGELKLEIAKSLVEDYSKIQGDIEELMDFLQASKKYHLNRRFAYREYLIKKIQLRDSQASALINAAATQILNLINKMERAGHLHESHLGMLLDRAEAEINSVKQKFNHDLKQEKQKLQQKLITKRKWKMLQKKEHWKEQLSIGNPFKTSREITDYLSHWKTVLSDHAIEFEELTEKLDNEGSEELRDLIFNLAEKTVEELKLVQYGVFVQELIKLSVPKMFLLEVVEEHKKEFIVKHEHLEREEREKSMAAEELLQLTRQKLSQELESSLSQQKKLRNWEQSVFMQLLTLPLSLSEEELLKMRQEFHCCFSQLDSSLALPKIRARALLQAFELEWRDAQLLKVSQNLAMTNKQLHSKTKKTGSKNRNKIDVLKKSLQDKIFIYEDSVKVENLTKVNYELLLEKDCQIHDLENKLGEYIASLEFQKSVKKCKMLELYAAVISVQGLLLENLSMSKTLSKSECIQILEAHNPEIEELDRKLEYEMLHKESAQQQQHLMNKQRWTPDGLRPSSEVVETNVDRQVTFLLRQAMNRCMQFINLHRQSLRDEQWNCMVLEDLLENIEMDAFLALYSQELRLAGYLARLTRVPMGMLHRILNLLLPSSSQSEVLSILDSIGVYSDGAAESDSNADESGSCKKRRNQECWQALEYKVREDLVTKSLERILSLNRRKDSLIKKKQLALLERPSFVHLESLPTRPPVERSSPHASFNSVTAEAIEVLDTGEKIFLFRDRSDPTLSLHNYPRKKKKNFLNSKKAARANIN

Target ORF information:

RefSeq Version	XM_025149755.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus EvC ciliary complex subunit 2 (EVC2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025149755.1

ORF Insert Sequence:

ATGAATTGTC TGCGGATGAC AGCACTGCAG TTGCCTGTGG TGGTTCAAGC AAGTGAAGTC 
CCTGATCTGA TCTTGACTTC TGCAGCCTCG GAAGATCAGT GGAGCCATTC AATATTTGCT 
CTGATACCCT CATGGACAAG GAAGATTTTG TTCAAACGAG AGTCTTCTGT TAACCACCAG 
CTGTCTTTTC CCCTTCCACC ACAGCCTGAA GATGTGTCCA ACTTGTCAGC ATCCTCTGGA 
TTTGGAATAA CCCTTCAGAA ATGTGCTGTG GTGGATGTTG AACATGACCC TCAAACTGCA 
TACATTAGAC TTCTGATTAA TAACACCAAC TCACTGCTCT CAAGAAACAT TACTGATCTT 
ATCCTTCTGG ACAATATCAC CGGTCTACAT GTTCAAAAAG ATAGTGGTAA TGAGACAAGA 
GATGGGATGC AAATTTACAG GAAAAGATTC TTGCAAGTTG GGGAATATTT TGCAATCAAC 
TATACTGCAC TTCTTGATGC AAAGAAAACG TGGAAAGGTA GGGTCTTGAC ACTGCCTGCA 
CAGCTGACTT TCAGGAGTTC ATCACAGAAT CAAACAGATC TTGTATCATT GACCACATTG 
TTCACAATAA CTGAAGAAGA AAAGACCGAG GTCTCCTACA GCCATGCCAT CCATGCTTCA 
GGGTTCTTCA TTGCTTTTTT TGTTTCCCTG GTATTGACAT GTGCTGTTTT CTTCATTCTT 
TACCGAACCC AATTATTAAG ATGGAGTTTC TATAGTGAAA ACCAGGAAAT ACAACATGAA 
CTCAGTCAAA ATCACAAACT GGAGCATTCT CAGTTCAGTT CAGGTGACCT CTTCAGTGAA 
GATGTTATTA TGAATGACCA AATCATTGAT ATACTGACCT TTGAAGAGTC GGGGAATATG 
CTTCAGGCTT TAGAAGACTT GGAGGTTGCC AGTCTGACTC GGGCAGATGC TGATTTGGAG 
GCATATCGAA TGCAGATCTG TAAAGAAGTG ATTGCGATAA TGATGAAGAA CATGGTTTTA 
AGTCACAGCC TCATTCCGCA TGTGGAGAAG AGGATGAGTT CAATTTTCAA AAAGCAGTTT 
CTTACAATGG AGAGAGAGTT TCAGGAAGAG TACGAAAGGA AGGTTGTGGC TTTAACAGCT 
GAATGTAATC TGGAAACAAG GAAGAAGATG GAGGCTCAAC GCCAGAAAGA GAGAGCTGCA 
AATGAAGAGG CTGAAGAGTT AATAAGGAAA GTGAATGAAA TGCCTGCTGT AGAATACAAA 
AGTCTTTTGG ATAGACTTCA CAGTCTGGAG CAGGACCATC TGAAGAGGTT CCTTCTGGTA 
AAGCAAGAGG AGTATTTTGC AAAGGCTTAC AGACAACTGG CTGTTTCCCG GAGAAAGGAG 
CTCCATAACA TCTTCTTTAC ACAGATAACA AATGCTACTT TCAAGGGAGA ACTGAAACTG 
GAAATAGCAA AATCATTAGT GGAAGATTAC TCTAAAATAC AGGGAGACAT TGAAGAGCTA 
ATGGATTTTT TGCAAGCCAG CAAGAAGTAT CACCTGAACA GAAGATTTGC TTACAGAGAA 
TATCTTATAA AAAAGATACA GTTAAGGGAT TCTCAGGCCT CTGCTCTTAT AAATGCAGCA 
GCAACTCAGA TATTGAATCT CATTAATAAG ATGGAAAGGG CGGGTCATCT ACATGAAAGT 
CATTTGGGAA TGCTGCTGGA CCGAGCTGAG GCTGAAATTA ATTCTGTTAA ACAAAAATTC 
AATCATGATT TAAAACAAGA AAAGCAAAAG CTTCAGCAGA AACTCATTAC CAAGCGAAAG 
TGGAAAATGT TGCAAAAGAA AGAGCATTGG AAGGAGCAGC TGTCCATAGG GAACCCCTTC 
AAAACCTCAA GGGAGATCAC TGACTACCTG AGCCACTGGA AAACTGTTCT GAGTGATCAT 
GCCATTGAAT TTGAAGAACT TACTGAAAAG CTGGATAATG AAGGCAGTGA AGAGCTGAGA 
GACCTTATAT TTAATCTAGC AGAGAAAACT GTTGAAGAAC TTAAACTTGT TCAATATGGA 
GTATTTGTGC AAGAACTGAT AAAGCTCAGT GTGCCAAAGA TGTTCCTGCT GGAAGTTGTA 
GAGGAGCATA AAAAAGAATT CATTGTTAAA CATGAACACC TTGAAAGGGA AGAACGTGAA 
AAGAGCATGG CTGCTGAAGA GTTGCTTCAG CTCACCAGGC AAAAACTAAG CCAAGAGCTG 
GAAAGTAGTC TTTCACAACA GAAAAAATTA AGGAACTGGG AACAATCAGT ATTCATGCAG 
CTTCTAACTT TGCCCCTTTC ACTGTCTGAA GAGGAATTGC TCAAAATGAG GCAAGAGTTC 
CACTGCTGCT TTTCACAGCT GGACAGCAGT TTGGCTCTGC CCAAGATAAG AGCAAGAGCT 
TTACTTCAGG CTTTTGAGCT GGAGTGGAGG GATGCGCAGC TGCTGAAAGT CAGTCAGAAT 
CTAGCAATGA CCAATAAGCA GCTGCATTCT AAAACGAAAA AAACAGGATC CAAGAATCGA 
AATAAGATTG ATGTTTTGAA GAAATCTTTA CAGGACAAAA TTTTCATTTA TGAAGACTCA 
GTGAAAGTTG AAAACTTGAC TAAGGTCAAT TATGAGTTGC TACTTGAGAA AGACTGTCAG 
ATACATGATC TGGAAAATAA ACTTGGAGAA TATATTGCTT CTTTAGAGTT TCAAAAGAGT 
GTTAAAAAAT GCAAAATGTT GGAGCTCTAT GCAGCAGTCA TAAGTGTGCA AGGTTTGCTC 
TTGGAAAACC TGAGCATGTC AAAAACGCTG TCAAAATCGG AATGTATTCA GATTTTAGAA 
GCTCATAATC CGGAGATTGA AGAACTTGAC AGGAAGCTGG AGTATGAAAT GCTGCATAAA 
GAGTCAGCAC AACAGCAGCA ACATCTAATG AATAAGCAGA GGTGGACACC GGATGGATTG 
AGACCTTCAA GTGAAGTCGT AGAAACAAAT GTAGACAGAC AGGTGACATT TCTGCTAAGA 
CAGGCCATGA ATAGATGTAT GCAGTTTATA AATCTGCACC GGCAAAGCTT GAGAGATGAG 
CAATGGAATT GTATGGTGCT TGAAGATCTC TTGGAAAATA TAGAAATGGA TGCATTTTTG 
GCACTTTATA GTCAGGAGCT CCGACTGGCA GGTTATTTAG CCAGATTGAC AAGGGTACCA 
ATGGGGATGT TGCACAGGAT TCTGAATTTG TTGCTGCCTT CCAGTTCGCA AAGTGAGGTT 
CTTTCTATCC TTGATTCCAT TGGTGTATAT TCAGATGGTG CTGCTGAGAG TGATAGTAAT 
GCAGATGAGT CTGGCAGCTG TAAAAAAAGG AGGAACCAAG AGTGTTGGCA AGCATTGGAG 
TACAAAGTAA GGGAAGATCT GGTAACCAAA AGTTTGGAAA GGATCCTTTC TCTAAATAGG 
AGAAAAGACA GCTTGATAAA GAAGAAGCAA CTTGCCCTTC TGGAAAGACC ATCATTCGTT 
CACCTTGAAA GTTTGCCTAC ACGTCCTCCT GTGGAACGGT CCAGTCCACA TGCTTCTTTT 
AACAGTGTGA CAGCTGAAGC CATCGAGGTA TTAGACACAG GGGAAAAGAT ATTTTTGTTT 
CGTGACCGGA GTGATCCAAC ACTTTCTCTT CATAACTATC CGAGAAAAAA GAAGAAGAAC 
TTTCTTAATT CCAAAAAGGC TGCTCGTGCA AATATCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EVC2 ( XM_015285794.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015141280.1 Gallus gallus Ellis van Creveld syndrome 2 (EVC2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa35734
Clone ID Related Accession (Same CDS sequence)	XM_025149755.1 , XM_015285794.1 , XM_025149754.1
Accession Version	XM_015285794.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	limbin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455863.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTGTC TGCGGATGAC AGCACTGCAG TTGCCTGTGG TGGTTCAAGC AAGTGAAGTC 
CCTGATCTGA TCTTGACTTC TGCAGCCTCG GAAGATCAGT GGAGCCATTC AATATTTGCT 
CTGATACCCT CATGGACAAG GAAGATTTTG TTCAAACGAG AGTCTTCTGT TAACCACCAG 
CTGTCTTTTC CCCTTCCACC ACAGCCTGAA GATGTGTCCA ACTTGTCAGC ATCCTCTGGA 
TTTGGAATAA CCCTTCAGAA ATGTGCTGTG GTGGATGTTG AACATGACCC TCAAACTGCA 
TACATTAGAC TTCTGATTAA TAACACCAAC TCACTGCTCT CAAGAAACAT TACTGATCTT 
ATCCTTCTGG ACAATATCAC CGGTCTACAT GTTCAAAAAG ATAGTGGTAA TGAGACAAGA 
GATGGGATGC AAATTTACAG GAAAAGATTC TTGCAAGTTG GGGAATATTT TGCAATCAAC 
TATACTGCAC TTCTTGATGC AAAGAAAACG TGGAAAGGTA GGGTCTTGAC ACTGCCTGCA 
CAGCTGACTT TCAGGAGTTC ATCACAGAAT CAAACAGATC TTGTATCATT GACCACATTG 
TTCACAATAA CTGAAGAAGA AAAGACCGAG GTCTCCTACA GCCATGCCAT CCATGCTTCA 
GGGTTCTTCA TTGCTTTTTT TGTTTCCCTG GTATTGACAT GTGCTGTTTT CTTCATTCTT 
TACCGAACCC AATTATTAAG ATGGAGTTTC TATAGTGAAA ACCAGGAAAT ACAACATGAA 
CTCAGTCAAA ATCACAAACT GGAGCATTCT CAGTTCAGTT CAGGTGACCT CTTCAGTGAA 
GATGTTATTA TGAATGACCA AATCATTGAT ATACTGACCT TTGAAGAGTC GGGGAATATG 
CTTCAGGCTT TAGAAGACTT GGAGGTTGCC AGTCTGACTC GGGCAGATGC TGATTTGGAG 
GCATATCGAA TGCAGATCTG TAAAGAAGTG ATTGCGATAA TGATGAAGAA CATGGTTTTA 
AGTCACAGCC TCATTCCGCA TGTGGAGAAG AGGATGAGTT CAATTTTCAA AAAGCAGTTT 
CTTACAATGG AGAGAGAGTT TCAGGAAGAG TACGAAAGGA AGGTTGTGGC TTTAACAGCT 
GAATGTAATC TGGAAACAAG GAAGAAGATG GAGGCTCAAC GCCAGAAAGA GAGAGCTGCA 
AATGAAGAGG CTGAAGAGTT AATAAGGAAA GTGAATGAAA TGCCTGCTGT AGAATACAAA 
AGTCTTTTGG ATAGACTTCA CAGTCTGGAG CAGGACCATC TGAAGAGGTT CCTTCTGGTA 
AAGCAAGAGG AGTATTTTGC AAAGGCTTAC AGACAACTGG CTGTTTCCCG GAGAAAGGAG 
CTCCATAACA TCTTCTTTAC ACAGATAACA AATGCTACTT TCAAGGGAGA ACTGAAACTG 
GAAATAGCAA AATCATTAGT GGAAGATTAC TCTAAAATAC AGGGAGACAT TGAAGAGCTA 
ATGGATTTTT TGCAAGCCAG CAAGAAGTAT CACCTGAACA GAAGATTTGC TTACAGAGAA 
TATCTTATAA AAAAGATACA GTTAAGGGAT TCTCAGGCCT CTGCTCTTAT AAATGCAGCA 
GCAACTCAGA TATTGAATCT CATTAATAAG ATGGAAAGGG CGGGTCATCT ACATGAAAGT 
CATTTGGGAA TGCTGCTGGA CCGAGCTGAG GCTGAAATTA ATTCTGTTAA ACAAAAATTC 
AATCATGATT TAAAACAAGA AAAGCAAAAG CTTCAGCAGA AACTCATTAC CAAGCGAAAG 
TGGAAAATGT TGCAAAAGAA AGAGCATTGG AAGGAGCAGC TGTCCATAGG GAACCCCTTC 
AAAACCTCAA GGGAGATCAC TGACTACCTG AGCCACTGGA AAACTGTTCT GAGTGATCAT 
GCCATTGAAT TTGAAGAACT TACTGAAAAG CTGGATAATG AAGGCAGTGA AGAGCTGAGA 
GACCTTATAT TTAATCTAGC AGAGAAAACT GTTGAAGAAC TTAAACTTGT TCAATATGGA 
GTATTTGTGC AAGAACTGAT AAAGCTCAGT GTGCCAAAGA TGTTCCTGCT GGAAGTTGTA 
GAGGAGCATA AAAAAGAATT CATTGTTAAA CATGAACACC TTGAAAGGGA AGAACGTGAA 
AAGAGCATGG CTGCTGAAGA GTTGCTTCAG CTCACCAGGC AAAAACTAAG CCAAGAGCTG 
GAAAGTAGTC TTTCACAACA GAAAAAATTA AGGAACTGGG AACAATCAGT ATTCATGCAG 
CTTCTAACTT TGCCCCTTTC ACTGTCTGAA GAGGAATTGC TCAAAATGAG GCAAGAGTTC 
CACTGCTGCT TTTCACAGCT GGACAGCAGT TTGGCTCTGC CCAAGATAAG AGCAAGAGCT 
TTACTTCAGG CTTTTGAGCT GGAGTGGAGG GATGCGCAGC TGCTGAAAGT CAGTCAGAAT 
CTAGCAATGA CCAATAAGCA GCTGCATTCT AAAACGAAAA AAACAGGATC CAAGAATCGA 
AATAAGATTG ATGTTTTGAA GAAATCTTTA CAGGACAAAA TTTTCATTTA TGAAGACTCA 
GTGAAAGTTG AAAACTTGAC TAAGGTCAAT TATGAGTTGC TACTTGAGAA AGACTGTCAG 
ATACATGATC TGGAAAATAA ACTTGGAGAA TATATTGCTT CTTTAGAGTT TCAAAAGAGT 
GTTAAAAAAT GCAAAATGTT GGAGCTCTAT GCAGCAGTCA TAAGTGTGCA AGGTTTGCTC 
TTGGAAAACC TGAGCATGTC AAAAACGCTG TCAAAATCGG AATGTATTCA GATTTTAGAA 
GCTCATAATC CGGAGATTGA AGAACTTGAC AGGAAGCTGG AGTATGAAAT GCTGCATAAA 
GAGTCAGCAC AACAGCAGCA ACATCTAATG AATAAGCAGA GGTGGACACC GGATGGATTG 
AGACCTTCAA GTGAAGTCGT AGAAACAAAT GTAGACAGAC AGGTGACATT TCTGCTAAGA 
CAGGCCATGA ATAGATGTAT GCAGTTTATA AATCTGCACC GGCAAAGCTT GAGAGATGAG 
CAATGGAATT GTATGGTGCT TGAAGATCTC TTGGAAAATA TAGAAATGGA TGCATTTTTG 
GCACTTTATA GTCAGGAGCT CCGACTGGCA GGTTATTTAG CCAGATTGAC AAGGGTACCA 
ATGGGGATGT TGCACAGGAT TCTGAATTTG TTGCTGCCTT CCAGTTCGCA AAGTGAGGTT 
CTTTCTATCC TTGATTCCAT TGGTGTATAT TCAGATGGTG CTGCTGAGAG TGATAGTAAT 
GCAGATGAGT CTGGCAGCTG TAAAAAAAGG AGGAACCAAG AGTGTTGGCA AGCATTGGAG 
TACAAAGTAA GGGAAGATCT GGTAACCAAA AGTTTGGAAA GGATCCTTTC TCTAAATAGG 
AGAAAAGACA GCTTGATAAA GAAGAAGCAA CTTGCCCTTC TGGAAAGACC ATCATTCGTT 
CACCTTGAAA GTTTGCCTAC ACGTCCTCCT GTGGAACGGT CCAGTCCACA TGCTTCTTTT 
AACAGTGTGA CAGCTGAAGC CATCGAGGTA TTAGACACAG GGGAAAAGAT ATTTTTGTTT 
CGTGACCGGA GTGATCCAAC ACTTTCTCTT CATAACTATC CGAGAAAAAA GAAGAAGAAC 
TTTCTTAATT CCAAAAAGGC TGCTCGTGCA AATATCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015141280.1
CDS	1656..5357
Translation	MNCLRMTALQLPVVVQASEVPDLILTSAASEDQWSHSIFALIPSWTRKILFKRESSVNHQLSFPLPPQPEDVSNLSASSGFGITLQKCAVVDVEHDPQTAYIRLLINNTNSLLSRNITDLILLDNITGLHVQKDSGNETRDGMQIYRKRFLQVGEYFAINYTALLDAKKTWKGRVLTLPAQLTFRSSSQNQTDLVSLTTLFTITEEEKTEVSYSHAIHASGFFIAFFVSLVLTCAVFFILYRTQLLRWSFYSENQEIQHELSQNHKLEHSQFSSGDLFSEDVIMNDQIIDILTFEESGNMLQALEDLEVASLTRADADLEAYRMQICKEVIAIMMKNMVLSHSLIPHVEKRMSSIFKKQFLTMEREFQEEYERKVVALTAECNLETRKKMEAQRQKERAANEEAEELIRKVNEMPAVEYKSLLDRLHSLEQDHLKRFLLVKQEEYFAKAYRQLAVSRRKELHNIFFTQITNATFKGELKLEIAKSLVEDYSKIQGDIEELMDFLQASKKYHLNRRFAYREYLIKKIQLRDSQASALINAAATQILNLINKMERAGHLHESHLGMLLDRAEAEINSVKQKFNHDLKQEKQKLQQKLITKRKWKMLQKKEHWKEQLSIGNPFKTSREITDYLSHWKTVLSDHAIEFEELTEKLDNEGSEELRDLIFNLAEKTVEELKLVQYGVFVQELIKLSVPKMFLLEVVEEHKKEFIVKHEHLEREEREKSMAAEELLQLTRQKLSQELESSLSQQKKLRNWEQSVFMQLLTLPLSLSEEELLKMRQEFHCCFSQLDSSLALPKIRARALLQAFELEWRDAQLLKVSQNLAMTNKQLHSKTKKTGSKNRNKIDVLKKSLQDKIFIYEDSVKVENLTKVNYELLLEKDCQIHDLENKLGEYIASLEFQKSVKKCKMLELYAAVISVQGLLLENLSMSKTLSKSECIQILEAHNPEIEELDRKLEYEMLHKESAQQQQHLMNKQRWTPDGLRPSSEVVETNVDRQVTFLLRQAMNRCMQFINLHRQSLRDEQWNCMVLEDLLENIEMDAFLALYSQELRLAGYLARLTRVPMGMLHRILNLLLPSSSQSEVLSILDSIGVYSDGAAESDSNADESGSCKKRRNQECWQALEYKVREDLVTKSLERILSLNRRKDSLIKKKQLALLERPSFVHLESLPTRPPVERSSPHASFNSVTAEAIEVLDTGEKIFLFRDRSDPTLSLHNYPRKKKKNFLNSKKAARANIN

Target ORF information:

RefSeq Version	XM_015285794.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ellis van Creveld syndrome 2 (EVC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285794.1

ORF Insert Sequence:

ATGAATTGTC TGCGGATGAC AGCACTGCAG TTGCCTGTGG TGGTTCAAGC AAGTGAAGTC 
CCTGATCTGA TCTTGACTTC TGCAGCCTCG GAAGATCAGT GGAGCCATTC AATATTTGCT 
CTGATACCCT CATGGACAAG GAAGATTTTG TTCAAACGAG AGTCTTCTGT TAACCACCAG 
CTGTCTTTTC CCCTTCCACC ACAGCCTGAA GATGTGTCCA ACTTGTCAGC ATCCTCTGGA 
TTTGGAATAA CCCTTCAGAA ATGTGCTGTG GTGGATGTTG AACATGACCC TCAAACTGCA 
TACATTAGAC TTCTGATTAA TAACACCAAC TCACTGCTCT CAAGAAACAT TACTGATCTT 
ATCCTTCTGG ACAATATCAC CGGTCTACAT GTTCAAAAAG ATAGTGGTAA TGAGACAAGA 
GATGGGATGC AAATTTACAG GAAAAGATTC TTGCAAGTTG GGGAATATTT TGCAATCAAC 
TATACTGCAC TTCTTGATGC AAAGAAAACG TGGAAAGGTA GGGTCTTGAC ACTGCCTGCA 
CAGCTGACTT TCAGGAGTTC ATCACAGAAT CAAACAGATC TTGTATCATT GACCACATTG 
TTCACAATAA CTGAAGAAGA AAAGACCGAG GTCTCCTACA GCCATGCCAT CCATGCTTCA 
GGGTTCTTCA TTGCTTTTTT TGTTTCCCTG GTATTGACAT GTGCTGTTTT CTTCATTCTT 
TACCGAACCC AATTATTAAG ATGGAGTTTC TATAGTGAAA ACCAGGAAAT ACAACATGAA 
CTCAGTCAAA ATCACAAACT GGAGCATTCT CAGTTCAGTT CAGGTGACCT CTTCAGTGAA 
GATGTTATTA TGAATGACCA AATCATTGAT ATACTGACCT TTGAAGAGTC GGGGAATATG 
CTTCAGGCTT TAGAAGACTT GGAGGTTGCC AGTCTGACTC GGGCAGATGC TGATTTGGAG 
GCATATCGAA TGCAGATCTG TAAAGAAGTG ATTGCGATAA TGATGAAGAA CATGGTTTTA 
AGTCACAGCC TCATTCCGCA TGTGGAGAAG AGGATGAGTT CAATTTTCAA AAAGCAGTTT 
CTTACAATGG AGAGAGAGTT TCAGGAAGAG TACGAAAGGA AGGTTGTGGC TTTAACAGCT 
GAATGTAATC TGGAAACAAG GAAGAAGATG GAGGCTCAAC GCCAGAAAGA GAGAGCTGCA 
AATGAAGAGG CTGAAGAGTT AATAAGGAAA GTGAATGAAA TGCCTGCTGT AGAATACAAA 
AGTCTTTTGG ATAGACTTCA CAGTCTGGAG CAGGACCATC TGAAGAGGTT CCTTCTGGTA 
AAGCAAGAGG AGTATTTTGC AAAGGCTTAC AGACAACTGG CTGTTTCCCG GAGAAAGGAG 
CTCCATAACA TCTTCTTTAC ACAGATAACA AATGCTACTT TCAAGGGAGA ACTGAAACTG 
GAAATAGCAA AATCATTAGT GGAAGATTAC TCTAAAATAC AGGGAGACAT TGAAGAGCTA 
ATGGATTTTT TGCAAGCCAG CAAGAAGTAT CACCTGAACA GAAGATTTGC TTACAGAGAA 
TATCTTATAA AAAAGATACA GTTAAGGGAT TCTCAGGCCT CTGCTCTTAT AAATGCAGCA 
GCAACTCAGA TATTGAATCT CATTAATAAG ATGGAAAGGG CGGGTCATCT ACATGAAAGT 
CATTTGGGAA TGCTGCTGGA CCGAGCTGAG GCTGAAATTA ATTCTGTTAA ACAAAAATTC 
AATCATGATT TAAAACAAGA AAAGCAAAAG CTTCAGCAGA AACTCATTAC CAAGCGAAAG 
TGGAAAATGT TGCAAAAGAA AGAGCATTGG AAGGAGCAGC TGTCCATAGG GAACCCCTTC 
AAAACCTCAA GGGAGATCAC TGACTACCTG AGCCACTGGA AAACTGTTCT GAGTGATCAT 
GCCATTGAAT TTGAAGAACT TACTGAAAAG CTGGATAATG AAGGCAGTGA AGAGCTGAGA 
GACCTTATAT TTAATCTAGC AGAGAAAACT GTTGAAGAAC TTAAACTTGT TCAATATGGA 
GTATTTGTGC AAGAACTGAT AAAGCTCAGT GTGCCAAAGA TGTTCCTGCT GGAAGTTGTA 
GAGGAGCATA AAAAAGAATT CATTGTTAAA CATGAACACC TTGAAAGGGA AGAACGTGAA 
AAGAGCATGG CTGCTGAAGA GTTGCTTCAG CTCACCAGGC AAAAACTAAG CCAAGAGCTG 
GAAAGTAGTC TTTCACAACA GAAAAAATTA AGGAACTGGG AACAATCAGT ATTCATGCAG 
CTTCTAACTT TGCCCCTTTC ACTGTCTGAA GAGGAATTGC TCAAAATGAG GCAAGAGTTC 
CACTGCTGCT TTTCACAGCT GGACAGCAGT TTGGCTCTGC CCAAGATAAG AGCAAGAGCT 
TTACTTCAGG CTTTTGAGCT GGAGTGGAGG GATGCGCAGC TGCTGAAAGT CAGTCAGAAT 
CTAGCAATGA CCAATAAGCA GCTGCATTCT AAAACGAAAA AAACAGGATC CAAGAATCGA 
AATAAGATTG ATGTTTTGAA GAAATCTTTA CAGGACAAAA TTTTCATTTA TGAAGACTCA 
GTGAAAGTTG AAAACTTGAC TAAGGTCAAT TATGAGTTGC TACTTGAGAA AGACTGTCAG 
ATACATGATC TGGAAAATAA ACTTGGAGAA TATATTGCTT CTTTAGAGTT TCAAAAGAGT 
GTTAAAAAAT GCAAAATGTT GGAGCTCTAT GCAGCAGTCA TAAGTGTGCA AGGTTTGCTC 
TTGGAAAACC TGAGCATGTC AAAAACGCTG TCAAAATCGG AATGTATTCA GATTTTAGAA 
GCTCATAATC CGGAGATTGA AGAACTTGAC AGGAAGCTGG AGTATGAAAT GCTGCATAAA 
GAGTCAGCAC AACAGCAGCA ACATCTAATG AATAAGCAGA GGTGGACACC GGATGGATTG 
AGACCTTCAA GTGAAGTCGT AGAAACAAAT GTAGACAGAC AGGTGACATT TCTGCTAAGA 
CAGGCCATGA ATAGATGTAT GCAGTTTATA AATCTGCACC GGCAAAGCTT GAGAGATGAG 
CAATGGAATT GTATGGTGCT TGAAGATCTC TTGGAAAATA TAGAAATGGA TGCATTTTTG 
GCACTTTATA GTCAGGAGCT CCGACTGGCA GGTTATTTAG CCAGATTGAC AAGGGTACCA 
ATGGGGATGT TGCACAGGAT TCTGAATTTG TTGCTGCCTT CCAGTTCGCA AAGTGAGGTT 
CTTTCTATCC TTGATTCCAT TGGTGTATAT TCAGATGGTG CTGCTGAGAG TGATAGTAAT 
GCAGATGAGT CTGGCAGCTG TAAAAAAAGG AGGAACCAAG AGTGTTGGCA AGCATTGGAG 
TACAAAGTAA GGGAAGATCT GGTAACCAAA AGTTTGGAAA GGATCCTTTC TCTAAATAGG 
AGAAAAGACA GCTTGATAAA GAAGAAGCAA CTTGCCCTTC TGGAAAGACC ATCATTCGTT 
CACCTTGAAA GTTTGCCTAC ACGTCCTCCT GTGGAACGGT CCAGTCCACA TGCTTCTTTT 
AACAGTGTGA CAGCTGAAGC CATCGAGGTA TTAGACACAG GGGAAAAGAT ATTTTTGTTT 
CGTGACCGGA GTGATCCAAC ACTTTCTCTT CATAACTATC CGAGAAAAAA GAAGAAGAAC 
TTTCTTAATT CCAAAAAGGC TGCTCGTGCA AATATCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EVC2 ( XM_025149754.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025005522.1 Gallus gallus EvC ciliary complex subunit 2 (EVC2), transcript variant X2, mRNA.

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CloneID	OGa35734
Clone ID Related Accession (Same CDS sequence)	XM_025149755.1 , XM_015285794.1 , XM_025149754.1
Accession Version	XM_025149754.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	limbin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTGTC TGCGGATGAC AGCACTGCAG TTGCCTGTGG TGGTTCAAGC AAGTGAAGTC 
CCTGATCTGA TCTTGACTTC TGCAGCCTCG GAAGATCAGT GGAGCCATTC AATATTTGCT 
CTGATACCCT CATGGACAAG GAAGATTTTG TTCAAACGAG AGTCTTCTGT TAACCACCAG 
CTGTCTTTTC CCCTTCCACC ACAGCCTGAA GATGTGTCCA ACTTGTCAGC ATCCTCTGGA 
TTTGGAATAA CCCTTCAGAA ATGTGCTGTG GTGGATGTTG AACATGACCC TCAAACTGCA 
TACATTAGAC TTCTGATTAA TAACACCAAC TCACTGCTCT CAAGAAACAT TACTGATCTT 
ATCCTTCTGG ACAATATCAC CGGTCTACAT GTTCAAAAAG ATAGTGGTAA TGAGACAAGA 
GATGGGATGC AAATTTACAG GAAAAGATTC TTGCAAGTTG GGGAATATTT TGCAATCAAC 
TATACTGCAC TTCTTGATGC AAAGAAAACG TGGAAAGGTA GGGTCTTGAC ACTGCCTGCA 
CAGCTGACTT TCAGGAGTTC ATCACAGAAT CAAACAGATC TTGTATCATT GACCACATTG 
TTCACAATAA CTGAAGAAGA AAAGACCGAG GTCTCCTACA GCCATGCCAT CCATGCTTCA 
GGGTTCTTCA TTGCTTTTTT TGTTTCCCTG GTATTGACAT GTGCTGTTTT CTTCATTCTT 
TACCGAACCC AATTATTAAG ATGGAGTTTC TATAGTGAAA ACCAGGAAAT ACAACATGAA 
CTCAGTCAAA ATCACAAACT GGAGCATTCT CAGTTCAGTT CAGGTGACCT CTTCAGTGAA 
GATGTTATTA TGAATGACCA AATCATTGAT ATACTGACCT TTGAAGAGTC GGGGAATATG 
CTTCAGGCTT TAGAAGACTT GGAGGTTGCC AGTCTGACTC GGGCAGATGC TGATTTGGAG 
GCATATCGAA TGCAGATCTG TAAAGAAGTG ATTGCGATAA TGATGAAGAA CATGGTTTTA 
AGTCACAGCC TCATTCCGCA TGTGGAGAAG AGGATGAGTT CAATTTTCAA AAAGCAGTTT 
CTTACAATGG AGAGAGAGTT TCAGGAAGAG TACGAAAGGA AGGTTGTGGC TTTAACAGCT 
GAATGTAATC TGGAAACAAG GAAGAAGATG GAGGCTCAAC GCCAGAAAGA GAGAGCTGCA 
AATGAAGAGG CTGAAGAGTT AATAAGGAAA GTGAATGAAA TGCCTGCTGT AGAATACAAA 
AGTCTTTTGG ATAGACTTCA CAGTCTGGAG CAGGACCATC TGAAGAGGTT CCTTCTGGTA 
AAGCAAGAGG AGTATTTTGC AAAGGCTTAC AGACAACTGG CTGTTTCCCG GAGAAAGGAG 
CTCCATAACA TCTTCTTTAC ACAGATAACA AATGCTACTT TCAAGGGAGA ACTGAAACTG 
GAAATAGCAA AATCATTAGT GGAAGATTAC TCTAAAATAC AGGGAGACAT TGAAGAGCTA 
ATGGATTTTT TGCAAGCCAG CAAGAAGTAT CACCTGAACA GAAGATTTGC TTACAGAGAA 
TATCTTATAA AAAAGATACA GTTAAGGGAT TCTCAGGCCT CTGCTCTTAT AAATGCAGCA 
GCAACTCAGA TATTGAATCT CATTAATAAG ATGGAAAGGG CGGGTCATCT ACATGAAAGT 
CATTTGGGAA TGCTGCTGGA CCGAGCTGAG GCTGAAATTA ATTCTGTTAA ACAAAAATTC 
AATCATGATT TAAAACAAGA AAAGCAAAAG CTTCAGCAGA AACTCATTAC CAAGCGAAAG 
TGGAAAATGT TGCAAAAGAA AGAGCATTGG AAGGAGCAGC TGTCCATAGG GAACCCCTTC 
AAAACCTCAA GGGAGATCAC TGACTACCTG AGCCACTGGA AAACTGTTCT GAGTGATCAT 
GCCATTGAAT TTGAAGAACT TACTGAAAAG CTGGATAATG AAGGCAGTGA AGAGCTGAGA 
GACCTTATAT TTAATCTAGC AGAGAAAACT GTTGAAGAAC TTAAACTTGT TCAATATGGA 
GTATTTGTGC AAGAACTGAT AAAGCTCAGT GTGCCAAAGA TGTTCCTGCT GGAAGTTGTA 
GAGGAGCATA AAAAAGAATT CATTGTTAAA CATGAACACC TTGAAAGGGA AGAACGTGAA 
AAGAGCATGG CTGCTGAAGA GTTGCTTCAG CTCACCAGGC AAAAACTAAG CCAAGAGCTG 
GAAAGTAGTC TTTCACAACA GAAAAAATTA AGGAACTGGG AACAATCAGT ATTCATGCAG 
CTTCTAACTT TGCCCCTTTC ACTGTCTGAA GAGGAATTGC TCAAAATGAG GCAAGAGTTC 
CACTGCTGCT TTTCACAGCT GGACAGCAGT TTGGCTCTGC CCAAGATAAG AGCAAGAGCT 
TTACTTCAGG CTTTTGAGCT GGAGTGGAGG GATGCGCAGC TGCTGAAAGT CAGTCAGAAT 
CTAGCAATGA CCAATAAGCA GCTGCATTCT AAAACGAAAA AAACAGGATC CAAGAATCGA 
AATAAGATTG ATGTTTTGAA GAAATCTTTA CAGGACAAAA TTTTCATTTA TGAAGACTCA 
GTGAAAGTTG AAAACTTGAC TAAGGTCAAT TATGAGTTGC TACTTGAGAA AGACTGTCAG 
ATACATGATC TGGAAAATAA ACTTGGAGAA TATATTGCTT CTTTAGAGTT TCAAAAGAGT 
GTTAAAAAAT GCAAAATGTT GGAGCTCTAT GCAGCAGTCA TAAGTGTGCA AGGTTTGCTC 
TTGGAAAACC TGAGCATGTC AAAAACGCTG TCAAAATCGG AATGTATTCA GATTTTAGAA 
GCTCATAATC CGGAGATTGA AGAACTTGAC AGGAAGCTGG AGTATGAAAT GCTGCATAAA 
GAGTCAGCAC AACAGCAGCA ACATCTAATG AATAAGCAGA GGTGGACACC GGATGGATTG 
AGACCTTCAA GTGAAGTCGT AGAAACAAAT GTAGACAGAC AGGTGACATT TCTGCTAAGA 
CAGGCCATGA ATAGATGTAT GCAGTTTATA AATCTGCACC GGCAAAGCTT GAGAGATGAG 
CAATGGAATT GTATGGTGCT TGAAGATCTC TTGGAAAATA TAGAAATGGA TGCATTTTTG 
GCACTTTATA GTCAGGAGCT CCGACTGGCA GGTTATTTAG CCAGATTGAC AAGGGTACCA 
ATGGGGATGT TGCACAGGAT TCTGAATTTG TTGCTGCCTT CCAGTTCGCA AAGTGAGGTT 
CTTTCTATCC TTGATTCCAT TGGTGTATAT TCAGATGGTG CTGCTGAGAG TGATAGTAAT 
GCAGATGAGT CTGGCAGCTG TAAAAAAAGG AGGAACCAAG AGTGTTGGCA AGCATTGGAG 
TACAAAGTAA GGGAAGATCT GGTAACCAAA AGTTTGGAAA GGATCCTTTC TCTAAATAGG 
AGAAAAGACA GCTTGATAAA GAAGAAGCAA CTTGCCCTTC TGGAAAGACC ATCATTCGTT 
CACCTTGAAA GTTTGCCTAC ACGTCCTCCT GTGGAACGGT CCAGTCCACA TGCTTCTTTT 
AACAGTGTGA CAGCTGAAGC CATCGAGGTA TTAGACACAG GGGAAAAGAT ATTTTTGTTT 
CGTGACCGGA GTGATCCAAC ACTTTCTCTT CATAACTATC CGAGAAAAAA GAAGAAGAAC 
TTTCTTAATT CCAAAAAGGC TGCTCGTGCA AATATCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025005522.1
CDS	214..3915
Translation	MNCLRMTALQLPVVVQASEVPDLILTSAASEDQWSHSIFALIPSWTRKILFKRESSVNHQLSFPLPPQPEDVSNLSASSGFGITLQKCAVVDVEHDPQTAYIRLLINNTNSLLSRNITDLILLDNITGLHVQKDSGNETRDGMQIYRKRFLQVGEYFAINYTALLDAKKTWKGRVLTLPAQLTFRSSSQNQTDLVSLTTLFTITEEEKTEVSYSHAIHASGFFIAFFVSLVLTCAVFFILYRTQLLRWSFYSENQEIQHELSQNHKLEHSQFSSGDLFSEDVIMNDQIIDILTFEESGNMLQALEDLEVASLTRADADLEAYRMQICKEVIAIMMKNMVLSHSLIPHVEKRMSSIFKKQFLTMEREFQEEYERKVVALTAECNLETRKKMEAQRQKERAANEEAEELIRKVNEMPAVEYKSLLDRLHSLEQDHLKRFLLVKQEEYFAKAYRQLAVSRRKELHNIFFTQITNATFKGELKLEIAKSLVEDYSKIQGDIEELMDFLQASKKYHLNRRFAYREYLIKKIQLRDSQASALINAAATQILNLINKMERAGHLHESHLGMLLDRAEAEINSVKQKFNHDLKQEKQKLQQKLITKRKWKMLQKKEHWKEQLSIGNPFKTSREITDYLSHWKTVLSDHAIEFEELTEKLDNEGSEELRDLIFNLAEKTVEELKLVQYGVFVQELIKLSVPKMFLLEVVEEHKKEFIVKHEHLEREEREKSMAAEELLQLTRQKLSQELESSLSQQKKLRNWEQSVFMQLLTLPLSLSEEELLKMRQEFHCCFSQLDSSLALPKIRARALLQAFELEWRDAQLLKVSQNLAMTNKQLHSKTKKTGSKNRNKIDVLKKSLQDKIFIYEDSVKVENLTKVNYELLLEKDCQIHDLENKLGEYIASLEFQKSVKKCKMLELYAAVISVQGLLLENLSMSKTLSKSECIQILEAHNPEIEELDRKLEYEMLHKESAQQQQHLMNKQRWTPDGLRPSSEVVETNVDRQVTFLLRQAMNRCMQFINLHRQSLRDEQWNCMVLEDLLENIEMDAFLALYSQELRLAGYLARLTRVPMGMLHRILNLLLPSSSQSEVLSILDSIGVYSDGAAESDSNADESGSCKKRRNQECWQALEYKVREDLVTKSLERILSLNRRKDSLIKKKQLALLERPSFVHLESLPTRPPVERSSPHASFNSVTAEAIEVLDTGEKIFLFRDRSDPTLSLHNYPRKKKKNFLNSKKAARANIN

Target ORF information:

RefSeq Version	XM_025149754.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus EvC ciliary complex subunit 2 (EVC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025149754.1

ORF Insert Sequence:

ATGAATTGTC TGCGGATGAC AGCACTGCAG TTGCCTGTGG TGGTTCAAGC AAGTGAAGTC 
CCTGATCTGA TCTTGACTTC TGCAGCCTCG GAAGATCAGT GGAGCCATTC AATATTTGCT 
CTGATACCCT CATGGACAAG GAAGATTTTG TTCAAACGAG AGTCTTCTGT TAACCACCAG 
CTGTCTTTTC CCCTTCCACC ACAGCCTGAA GATGTGTCCA ACTTGTCAGC ATCCTCTGGA 
TTTGGAATAA CCCTTCAGAA ATGTGCTGTG GTGGATGTTG AACATGACCC TCAAACTGCA 
TACATTAGAC TTCTGATTAA TAACACCAAC TCACTGCTCT CAAGAAACAT TACTGATCTT 
ATCCTTCTGG ACAATATCAC CGGTCTACAT GTTCAAAAAG ATAGTGGTAA TGAGACAAGA 
GATGGGATGC AAATTTACAG GAAAAGATTC TTGCAAGTTG GGGAATATTT TGCAATCAAC 
TATACTGCAC TTCTTGATGC AAAGAAAACG TGGAAAGGTA GGGTCTTGAC ACTGCCTGCA 
CAGCTGACTT TCAGGAGTTC ATCACAGAAT CAAACAGATC TTGTATCATT GACCACATTG 
TTCACAATAA CTGAAGAAGA AAAGACCGAG GTCTCCTACA GCCATGCCAT CCATGCTTCA 
GGGTTCTTCA TTGCTTTTTT TGTTTCCCTG GTATTGACAT GTGCTGTTTT CTTCATTCTT 
TACCGAACCC AATTATTAAG ATGGAGTTTC TATAGTGAAA ACCAGGAAAT ACAACATGAA 
CTCAGTCAAA ATCACAAACT GGAGCATTCT CAGTTCAGTT CAGGTGACCT CTTCAGTGAA 
GATGTTATTA TGAATGACCA AATCATTGAT ATACTGACCT TTGAAGAGTC GGGGAATATG 
CTTCAGGCTT TAGAAGACTT GGAGGTTGCC AGTCTGACTC GGGCAGATGC TGATTTGGAG 
GCATATCGAA TGCAGATCTG TAAAGAAGTG ATTGCGATAA TGATGAAGAA CATGGTTTTA 
AGTCACAGCC TCATTCCGCA TGTGGAGAAG AGGATGAGTT CAATTTTCAA AAAGCAGTTT 
CTTACAATGG AGAGAGAGTT TCAGGAAGAG TACGAAAGGA AGGTTGTGGC TTTAACAGCT 
GAATGTAATC TGGAAACAAG GAAGAAGATG GAGGCTCAAC GCCAGAAAGA GAGAGCTGCA 
AATGAAGAGG CTGAAGAGTT AATAAGGAAA GTGAATGAAA TGCCTGCTGT AGAATACAAA 
AGTCTTTTGG ATAGACTTCA CAGTCTGGAG CAGGACCATC TGAAGAGGTT CCTTCTGGTA 
AAGCAAGAGG AGTATTTTGC AAAGGCTTAC AGACAACTGG CTGTTTCCCG GAGAAAGGAG 
CTCCATAACA TCTTCTTTAC ACAGATAACA AATGCTACTT TCAAGGGAGA ACTGAAACTG 
GAAATAGCAA AATCATTAGT GGAAGATTAC TCTAAAATAC AGGGAGACAT TGAAGAGCTA 
ATGGATTTTT TGCAAGCCAG CAAGAAGTAT CACCTGAACA GAAGATTTGC TTACAGAGAA 
TATCTTATAA AAAAGATACA GTTAAGGGAT TCTCAGGCCT CTGCTCTTAT AAATGCAGCA 
GCAACTCAGA TATTGAATCT CATTAATAAG ATGGAAAGGG CGGGTCATCT ACATGAAAGT 
CATTTGGGAA TGCTGCTGGA CCGAGCTGAG GCTGAAATTA ATTCTGTTAA ACAAAAATTC 
AATCATGATT TAAAACAAGA AAAGCAAAAG CTTCAGCAGA AACTCATTAC CAAGCGAAAG 
TGGAAAATGT TGCAAAAGAA AGAGCATTGG AAGGAGCAGC TGTCCATAGG GAACCCCTTC 
AAAACCTCAA GGGAGATCAC TGACTACCTG AGCCACTGGA AAACTGTTCT GAGTGATCAT 
GCCATTGAAT TTGAAGAACT TACTGAAAAG CTGGATAATG AAGGCAGTGA AGAGCTGAGA 
GACCTTATAT TTAATCTAGC AGAGAAAACT GTTGAAGAAC TTAAACTTGT TCAATATGGA 
GTATTTGTGC AAGAACTGAT AAAGCTCAGT GTGCCAAAGA TGTTCCTGCT GGAAGTTGTA 
GAGGAGCATA AAAAAGAATT CATTGTTAAA CATGAACACC TTGAAAGGGA AGAACGTGAA 
AAGAGCATGG CTGCTGAAGA GTTGCTTCAG CTCACCAGGC AAAAACTAAG CCAAGAGCTG 
GAAAGTAGTC TTTCACAACA GAAAAAATTA AGGAACTGGG AACAATCAGT ATTCATGCAG 
CTTCTAACTT TGCCCCTTTC ACTGTCTGAA GAGGAATTGC TCAAAATGAG GCAAGAGTTC 
CACTGCTGCT TTTCACAGCT GGACAGCAGT TTGGCTCTGC CCAAGATAAG AGCAAGAGCT 
TTACTTCAGG CTTTTGAGCT GGAGTGGAGG GATGCGCAGC TGCTGAAAGT CAGTCAGAAT 
CTAGCAATGA CCAATAAGCA GCTGCATTCT AAAACGAAAA AAACAGGATC CAAGAATCGA 
AATAAGATTG ATGTTTTGAA GAAATCTTTA CAGGACAAAA TTTTCATTTA TGAAGACTCA 
GTGAAAGTTG AAAACTTGAC TAAGGTCAAT TATGAGTTGC TACTTGAGAA AGACTGTCAG 
ATACATGATC TGGAAAATAA ACTTGGAGAA TATATTGCTT CTTTAGAGTT TCAAAAGAGT 
GTTAAAAAAT GCAAAATGTT GGAGCTCTAT GCAGCAGTCA TAAGTGTGCA AGGTTTGCTC 
TTGGAAAACC TGAGCATGTC AAAAACGCTG TCAAAATCGG AATGTATTCA GATTTTAGAA 
GCTCATAATC CGGAGATTGA AGAACTTGAC AGGAAGCTGG AGTATGAAAT GCTGCATAAA 
GAGTCAGCAC AACAGCAGCA ACATCTAATG AATAAGCAGA GGTGGACACC GGATGGATTG 
AGACCTTCAA GTGAAGTCGT AGAAACAAAT GTAGACAGAC AGGTGACATT TCTGCTAAGA 
CAGGCCATGA ATAGATGTAT GCAGTTTATA AATCTGCACC GGCAAAGCTT GAGAGATGAG 
CAATGGAATT GTATGGTGCT TGAAGATCTC TTGGAAAATA TAGAAATGGA TGCATTTTTG 
GCACTTTATA GTCAGGAGCT CCGACTGGCA GGTTATTTAG CCAGATTGAC AAGGGTACCA 
ATGGGGATGT TGCACAGGAT TCTGAATTTG TTGCTGCCTT CCAGTTCGCA AAGTGAGGTT 
CTTTCTATCC TTGATTCCAT TGGTGTATAT TCAGATGGTG CTGCTGAGAG TGATAGTAAT 
GCAGATGAGT CTGGCAGCTG TAAAAAAAGG AGGAACCAAG AGTGTTGGCA AGCATTGGAG 
TACAAAGTAA GGGAAGATCT GGTAACCAAA AGTTTGGAAA GGATCCTTTC TCTAAATAGG 
AGAAAAGACA GCTTGATAAA GAAGAAGCAA CTTGCCCTTC TGGAAAGACC ATCATTCGTT 
CACCTTGAAA GTTTGCCTAC ACGTCCTCCT GTGGAACGGT CCAGTCCACA TGCTTCTTTT 
AACAGTGTGA CAGCTGAAGC CATCGAGGTA TTAGACACAG GGGAAAAGAT ATTTTTGTTT 
CGTGACCGGA GTGATCCAAC ACTTTCTCTT CATAACTATC CGAGAAAAAA GAAGAAGAAC 
TTTCTTAATT CCAAAAAGGC TGCTCGTGCA AATATCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EVC2 ( XM_015285795.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015141281.1 Gallus gallus Ellis van Creveld syndrome 2 (EVC2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa35735
Clone ID Related Accession (Same CDS sequence)	XM_015285795.1 , XM_015285795.2
Accession Version	XM_015285795.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	limbin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455863.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAAAA AGATAGTGGT AATGAGACAA GAGATGGGAT GCAAATTTAC AGGAAAAGAT 
TCTTGCAAGT TGGGGAATAT TTTGCAATCA ACTATACTGC ACTTCTTGAT GCAAAGAAAA 
CGTGGAAAGA ATCAAACAGA TCTTGTATCA TTGACCACAT TGTTCACAAT AACTGAAGAA 
GAAAAGACCG AGGTCTCCTA CAGCCATGCC ATCCATGCTT CAGGGTTCTT CATTGCTTTT 
TTTGTTTCCC TGGTATTGAC ATGTGCTGTT TTCTTCATTC TTTACCGAAC CCAATTATTA 
AGATGGAGTT TCTATAGTGA AAACCAGGAA ATACAACATG AACTCAGTCA AAATCACAAA 
CTGGAGCATT CTCAGTTCAG TTCAGGTGAC CTCTTCAGTG AAGATGTTAT TATGAATGAC 
CAAATCATTG ATATACTGAC CTTTGAAGAG TCGGGGAATA TGCTTCAGGC TTTAGAAGAC 
TTGGAGGTTG CCAGTCTGAC TCGGGCAGAT GCTGATTTGG AGGCATATCG AATGCAGATC 
TGTAAAGAAG TGATTGCGAT AATGATGAAG AACATGGTTT TAAGTCACAG CCTCATTCCG 
CATGTGGAGA AGAGGATGAG TTCAATTTTC AAAAAGCAGT TTCTTACAAT GGAGAGAGAG 
TTTCAGGAAG AGTACGAAAG GAAGGTTGTG GCTTTAACAG CTGAATGTAA TCTGGAAACA 
AGGAAGAAGA TGGAGGCTCA ACGCCAGAAA GAGAGAGCTG CAAATGAAGA GGCTGAAGAG 
TTAATAAGGA AAGTGAATGA AATGCCTGCT GTAGAATACA AAAGTCTTTT GGATAGACTT 
CACAGTCTGG AGCAGGACCA TCTGAAGAGG TTCCTTCTGG TAAAGCAAGA GGAGTATTTT 
GCAAAGGCTT ACAGACAACT GGCTGTTTCC CGGAGAAAGG AGCTCCATAA CATCTTCTTT 
ACACAGATAA CAAATGCTAC TTTCAAGGGA GAACTGAAAC TGGAAATAGC AAAATCATTA 
GTGGAAGATT ACTCTAAAAT ACAGGGAGAC ATTGAAGAGC TAATGGATTT TTTGCAAGCC 
AGCAAGAAGT ATCACCTGAA CAGAAGATTT GCTTACAGAG AATATCTTAT AAAAAAGATA 
CAGTTAAGGG ATTCTCAGGC CTCTGCTCTT ATAAATGCAG CAGCAACTCA GATATTGAAT 
CTCATTAATA AGATGGAAAG GGCGGGTCAT CTACATGAAA GTCATTTGGG AATGCTGCTG 
GACCGAGCTG AGGCTGAAAT TAATTCTGTT AAACAAAAAT TCAATCATGA TTTAAAACAA 
GAAAAGCAAA AGCTTCAGCA GAAACTCATT ACCAAGCGAA AGTGGAAAAT GTTGCAAAAG 
AAAGAGCATT GGAAGGAGCA GCTGTCCATA GGGAACCCCT TCAAAACCTC AAGGGAGATC 
ACTGACTACC TGAGCCACTG GAAAACTGTT CTGAGTGATC ATGCCATTGA ATTTGAAGAA 
CTTACTGAAA AGCTGGATAA TGAAGGCAGT GAAGAGCTGA GAGACCTTAT ATTTAATCTA 
GCAGAGAAAA CTGTTGAAGA ACTTAAACTT GTTCAATATG GAGTATTTGT GCAAGAACTG 
ATAAAGCTCA GTGTGCCAAA GATGTTCCTG CTGGAAGTTG TAGAGGAGCA TAAAAAAGAA 
TTCATTGTTA AACATGAACA CCTTGAAAGG GAAGAACGTG AAAAGAGCAT GGCTGCTGAA 
GAGTTGCTTC AGCTCACCAG GCAAAAACTA AGCCAAGAGC TGGAAAGTAG TCTTTCACAA 
CAGAAAAAAT TAAGGAACTG GGAACAATCA GTATTCATGC AGCTTCTAAC TTTGCCCCTT 
TCACTGTCTG AAGAGGAATT GCTCAAAATG AGGCAAGAGT TCCACTGCTG CTTTTCACAG 
CTGGACAGCA GTTTGGCTCT GCCCAAGATA AGAGCAAGAG CTTTACTTCA GGCTTTTGAG 
CTGGAGTGGA GGGATGCGCA GCTGCTGAAA GTCAGTCAGA ATCTAGCAAT GACCAATAAG 
CAGCTGCATT CTAAAACGAA AAAAACAGGA TCCAAGAATC GAAATAAGAT TGATGTTTTG 
AAGAAATCTT TACAGGACAA AATTTTCATT TATGAAGACT CAGTGAAAGT TGAAAACTTG 
ACTAAGGTCA ATTATGAGTT GCTACTTGAG AAAGACTGTC AGATACATGA TCTGGAAAAT 
AAACTTGGAG AATATATTGC TTCTTTAGAG TTTCAAAAGA GTGTTAAAAA ATGCAAAATG 
TTGGAGCTCT ATGCAGCAGT CATAAGTGTG CAAGGTTTGC TCTTGGAAAA CCTGAGCATG 
TCAAAAACGC TGTCAAAATC GGAATGTATT CAGATTTTAG AAGCTCATAA TCCGGAGATT 
GAAGAACTTG ACAGGAAGCT GGAGTATGAA ATGCTGCATA AAGAGTCAGC ACAACAGCAG 
CAACATCTAA TGAATAAGCA GAGGTGGACA CCGGATGGAT TGAGACCTTC AAGTGAAGTC 
GTAGAAACAA ATGTAGACAG ACAGGTGACA TTTCTGCTAA GACAGGCCAT GAATAGATGT 
ATGCAGTTTA TAAATCTGCA CCGGCAAAGC TTGAGAGATG AGCAATGGAA TTGTATGGTG 
CTTGAAGATC TCTTGGAAAA TATAGAAATG GATGCATTTT TGGCACTTTA TAGTCAGGAG 
CTCCGACTGG CAGGTTATTT AGCCAGATTG ACAAGGGTAC CAATGGGGAT GTTGCACAGG 
ATTCTGAATT TGTTGCTGCC TTCCAGTTCG CAAAGTGAGG TTCTTTCTAT CCTTGATTCC 
ATTGGTGTAT ATTCAGATGG TGCTGCTGAG AGTGATAGTA ATGCAGATGA GTCTGGCAGC 
TGTAAAAAAA GGAGGAACCA AGAGTGTTGG CAAGCATTGG AGTACAAAGT AAGGGAAGAT 
CTGGTAACCA AAAGTTTGGA AAGGATCCTT TCTCTAAATA GGAGAAAAGA CAGCTTGATA 
AAGAAGAAGC AACTTGCCCT TCTGGAAAGA CCATCATTCG TTCACCTTGA AAGTTTGCCT 
ACACGTCCTC CTGTGGAACG GTCCAGTCCA CATGCTTCTT TTAACAGTGT GACAGCTGAA 
GCCATCGAGG TATTAGACAC AGGGGAAAAG ATATTTTTGT TTCGTGACCG GAGTGATCCA 
ACACTTTCTC TTCATAACTA TCCGAGAAAA AAGAAGAAGA ACTTTCTTAA TTCCAAAAAG 
GCTGCTCGTG CAAATATCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015141281.1
CDS	104..3367
Translation	MFKKIVVMRQEMGCKFTGKDSCKLGNILQSTILHFLMQRKRGKNQTDLVSLTTLFTITEEEKTEVSYSHAIHASGFFIAFFVSLVLTCAVFFILYRTQLLRWSFYSENQEIQHELSQNHKLEHSQFSSGDLFSEDVIMNDQIIDILTFEESGNMLQALEDLEVASLTRADADLEAYRMQICKEVIAIMMKNMVLSHSLIPHVEKRMSSIFKKQFLTMEREFQEEYERKVVALTAECNLETRKKMEAQRQKERAANEEAEELIRKVNEMPAVEYKSLLDRLHSLEQDHLKRFLLVKQEEYFAKAYRQLAVSRRKELHNIFFTQITNATFKGELKLEIAKSLVEDYSKIQGDIEELMDFLQASKKYHLNRRFAYREYLIKKIQLRDSQASALINAAATQILNLINKMERAGHLHESHLGMLLDRAEAEINSVKQKFNHDLKQEKQKLQQKLITKRKWKMLQKKEHWKEQLSIGNPFKTSREITDYLSHWKTVLSDHAIEFEELTEKLDNEGSEELRDLIFNLAEKTVEELKLVQYGVFVQELIKLSVPKMFLLEVVEEHKKEFIVKHEHLEREEREKSMAAEELLQLTRQKLSQELESSLSQQKKLRNWEQSVFMQLLTLPLSLSEEELLKMRQEFHCCFSQLDSSLALPKIRARALLQAFELEWRDAQLLKVSQNLAMTNKQLHSKTKKTGSKNRNKIDVLKKSLQDKIFIYEDSVKVENLTKVNYELLLEKDCQIHDLENKLGEYIASLEFQKSVKKCKMLELYAAVISVQGLLLENLSMSKTLSKSECIQILEAHNPEIEELDRKLEYEMLHKESAQQQQHLMNKQRWTPDGLRPSSEVVETNVDRQVTFLLRQAMNRCMQFINLHRQSLRDEQWNCMVLEDLLENIEMDAFLALYSQELRLAGYLARLTRVPMGMLHRILNLLLPSSSQSEVLSILDSIGVYSDGAAESDSNADESGSCKKRRNQECWQALEYKVREDLVTKSLERILSLNRRKDSLIKKKQLALLERPSFVHLESLPTRPPVERSSPHASFNSVTAEAIEVLDTGEKIFLFRDRSDPTLSLHNYPRKKKKNFLNSKKAARANIN

Target ORF information:

RefSeq Version	XM_015285795.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ellis van Creveld syndrome 2 (EVC2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285795.1

ORF Insert Sequence:

ATGTTCAAAA AGATAGTGGT AATGAGACAA GAGATGGGAT GCAAATTTAC AGGAAAAGAT 
TCTTGCAAGT TGGGGAATAT TTTGCAATCA ACTATACTGC ACTTCTTGAT GCAAAGAAAA 
CGTGGAAAGA ATCAAACAGA TCTTGTATCA TTGACCACAT TGTTCACAAT AACTGAAGAA 
GAAAAGACCG AGGTCTCCTA CAGCCATGCC ATCCATGCTT CAGGGTTCTT CATTGCTTTT 
TTTGTTTCCC TGGTATTGAC ATGTGCTGTT TTCTTCATTC TTTACCGAAC CCAATTATTA 
AGATGGAGTT TCTATAGTGA AAACCAGGAA ATACAACATG AACTCAGTCA AAATCACAAA 
CTGGAGCATT CTCAGTTCAG TTCAGGTGAC CTCTTCAGTG AAGATGTTAT TATGAATGAC 
CAAATCATTG ATATACTGAC CTTTGAAGAG TCGGGGAATA TGCTTCAGGC TTTAGAAGAC 
TTGGAGGTTG CCAGTCTGAC TCGGGCAGAT GCTGATTTGG AGGCATATCG AATGCAGATC 
TGTAAAGAAG TGATTGCGAT AATGATGAAG AACATGGTTT TAAGTCACAG CCTCATTCCG 
CATGTGGAGA AGAGGATGAG TTCAATTTTC AAAAAGCAGT TTCTTACAAT GGAGAGAGAG 
TTTCAGGAAG AGTACGAAAG GAAGGTTGTG GCTTTAACAG CTGAATGTAA TCTGGAAACA 
AGGAAGAAGA TGGAGGCTCA ACGCCAGAAA GAGAGAGCTG CAAATGAAGA GGCTGAAGAG 
TTAATAAGGA AAGTGAATGA AATGCCTGCT GTAGAATACA AAAGTCTTTT GGATAGACTT 
CACAGTCTGG AGCAGGACCA TCTGAAGAGG TTCCTTCTGG TAAAGCAAGA GGAGTATTTT 
GCAAAGGCTT ACAGACAACT GGCTGTTTCC CGGAGAAAGG AGCTCCATAA CATCTTCTTT 
ACACAGATAA CAAATGCTAC TTTCAAGGGA GAACTGAAAC TGGAAATAGC AAAATCATTA 
GTGGAAGATT ACTCTAAAAT ACAGGGAGAC ATTGAAGAGC TAATGGATTT TTTGCAAGCC 
AGCAAGAAGT ATCACCTGAA CAGAAGATTT GCTTACAGAG AATATCTTAT AAAAAAGATA 
CAGTTAAGGG ATTCTCAGGC CTCTGCTCTT ATAAATGCAG CAGCAACTCA GATATTGAAT 
CTCATTAATA AGATGGAAAG GGCGGGTCAT CTACATGAAA GTCATTTGGG AATGCTGCTG 
GACCGAGCTG AGGCTGAAAT TAATTCTGTT AAACAAAAAT TCAATCATGA TTTAAAACAA 
GAAAAGCAAA AGCTTCAGCA GAAACTCATT ACCAAGCGAA AGTGGAAAAT GTTGCAAAAG 
AAAGAGCATT GGAAGGAGCA GCTGTCCATA GGGAACCCCT TCAAAACCTC AAGGGAGATC 
ACTGACTACC TGAGCCACTG GAAAACTGTT CTGAGTGATC ATGCCATTGA ATTTGAAGAA 
CTTACTGAAA AGCTGGATAA TGAAGGCAGT GAAGAGCTGA GAGACCTTAT ATTTAATCTA 
GCAGAGAAAA CTGTTGAAGA ACTTAAACTT GTTCAATATG GAGTATTTGT GCAAGAACTG 
ATAAAGCTCA GTGTGCCAAA GATGTTCCTG CTGGAAGTTG TAGAGGAGCA TAAAAAAGAA 
TTCATTGTTA AACATGAACA CCTTGAAAGG GAAGAACGTG AAAAGAGCAT GGCTGCTGAA 
GAGTTGCTTC AGCTCACCAG GCAAAAACTA AGCCAAGAGC TGGAAAGTAG TCTTTCACAA 
CAGAAAAAAT TAAGGAACTG GGAACAATCA GTATTCATGC AGCTTCTAAC TTTGCCCCTT 
TCACTGTCTG AAGAGGAATT GCTCAAAATG AGGCAAGAGT TCCACTGCTG CTTTTCACAG 
CTGGACAGCA GTTTGGCTCT GCCCAAGATA AGAGCAAGAG CTTTACTTCA GGCTTTTGAG 
CTGGAGTGGA GGGATGCGCA GCTGCTGAAA GTCAGTCAGA ATCTAGCAAT GACCAATAAG 
CAGCTGCATT CTAAAACGAA AAAAACAGGA TCCAAGAATC GAAATAAGAT TGATGTTTTG 
AAGAAATCTT TACAGGACAA AATTTTCATT TATGAAGACT CAGTGAAAGT TGAAAACTTG 
ACTAAGGTCA ATTATGAGTT GCTACTTGAG AAAGACTGTC AGATACATGA TCTGGAAAAT 
AAACTTGGAG AATATATTGC TTCTTTAGAG TTTCAAAAGA GTGTTAAAAA ATGCAAAATG 
TTGGAGCTCT ATGCAGCAGT CATAAGTGTG CAAGGTTTGC TCTTGGAAAA CCTGAGCATG 
TCAAAAACGC TGTCAAAATC GGAATGTATT CAGATTTTAG AAGCTCATAA TCCGGAGATT 
GAAGAACTTG ACAGGAAGCT GGAGTATGAA ATGCTGCATA AAGAGTCAGC ACAACAGCAG 
CAACATCTAA TGAATAAGCA GAGGTGGACA CCGGATGGAT TGAGACCTTC AAGTGAAGTC 
GTAGAAACAA ATGTAGACAG ACAGGTGACA TTTCTGCTAA GACAGGCCAT GAATAGATGT 
ATGCAGTTTA TAAATCTGCA CCGGCAAAGC TTGAGAGATG AGCAATGGAA TTGTATGGTG 
CTTGAAGATC TCTTGGAAAA TATAGAAATG GATGCATTTT TGGCACTTTA TAGTCAGGAG 
CTCCGACTGG CAGGTTATTT AGCCAGATTG ACAAGGGTAC CAATGGGGAT GTTGCACAGG 
ATTCTGAATT TGTTGCTGCC TTCCAGTTCG CAAAGTGAGG TTCTTTCTAT CCTTGATTCC 
ATTGGTGTAT ATTCAGATGG TGCTGCTGAG AGTGATAGTA ATGCAGATGA GTCTGGCAGC 
TGTAAAAAAA GGAGGAACCA AGAGTGTTGG CAAGCATTGG AGTACAAAGT AAGGGAAGAT 
CTGGTAACCA AAAGTTTGGA AAGGATCCTT TCTCTAAATA GGAGAAAAGA CAGCTTGATA 
AAGAAGAAGC AACTTGCCCT TCTGGAAAGA CCATCATTCG TTCACCTTGA AAGTTTGCCT 
ACACGTCCTC CTGTGGAACG GTCCAGTCCA CATGCTTCTT TTAACAGTGT GACAGCTGAA 
GCCATCGAGG TATTAGACAC AGGGGAAAAG ATATTTTTGT TTCGTGACCG GAGTGATCCA 
ACACTTTCTC TTCATAACTA TCCGAGAAAA AAGAAGAAGA ACTTTCTTAA TTCCAAAAAG 
GCTGCTCGTG CAAATATCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EVC2 ( XM_015285795.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015141281.1 Gallus gallus EvC ciliary complex subunit 2 (EVC2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OGa35735
Clone ID Related Accession (Same CDS sequence)	XM_015285795.1 , XM_015285795.2
Accession Version	XM_015285795.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	limbin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285795.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAAAA AGATAGTGGT AATGAGACAA GAGATGGGAT GCAAATTTAC AGGAAAAGAT 
TCTTGCAAGT TGGGGAATAT TTTGCAATCA ACTATACTGC ACTTCTTGAT GCAAAGAAAA 
CGTGGAAAGA ATCAAACAGA TCTTGTATCA TTGACCACAT TGTTCACAAT AACTGAAGAA 
GAAAAGACCG AGGTCTCCTA CAGCCATGCC ATCCATGCTT CAGGGTTCTT CATTGCTTTT 
TTTGTTTCCC TGGTATTGAC ATGTGCTGTT TTCTTCATTC TTTACCGAAC CCAATTATTA 
AGATGGAGTT TCTATAGTGA AAACCAGGAA ATACAACATG AACTCAGTCA AAATCACAAA 
CTGGAGCATT CTCAGTTCAG TTCAGGTGAC CTCTTCAGTG AAGATGTTAT TATGAATGAC 
CAAATCATTG ATATACTGAC CTTTGAAGAG TCGGGGAATA TGCTTCAGGC TTTAGAAGAC 
TTGGAGGTTG CCAGTCTGAC TCGGGCAGAT GCTGATTTGG AGGCATATCG AATGCAGATC 
TGTAAAGAAG TGATTGCGAT AATGATGAAG AACATGGTTT TAAGTCACAG CCTCATTCCG 
CATGTGGAGA AGAGGATGAG TTCAATTTTC AAAAAGCAGT TTCTTACAAT GGAGAGAGAG 
TTTCAGGAAG AGTACGAAAG GAAGGTTGTG GCTTTAACAG CTGAATGTAA TCTGGAAACA 
AGGAAGAAGA TGGAGGCTCA ACGCCAGAAA GAGAGAGCTG CAAATGAAGA GGCTGAAGAG 
TTAATAAGGA AAGTGAATGA AATGCCTGCT GTAGAATACA AAAGTCTTTT GGATAGACTT 
CACAGTCTGG AGCAGGACCA TCTGAAGAGG TTCCTTCTGG TAAAGCAAGA GGAGTATTTT 
GCAAAGGCTT ACAGACAACT GGCTGTTTCC CGGAGAAAGG AGCTCCATAA CATCTTCTTT 
ACACAGATAA CAAATGCTAC TTTCAAGGGA GAACTGAAAC TGGAAATAGC AAAATCATTA 
GTGGAAGATT ACTCTAAAAT ACAGGGAGAC ATTGAAGAGC TAATGGATTT TTTGCAAGCC 
AGCAAGAAGT ATCACCTGAA CAGAAGATTT GCTTACAGAG AATATCTTAT AAAAAAGATA 
CAGTTAAGGG ATTCTCAGGC CTCTGCTCTT ATAAATGCAG CAGCAACTCA GATATTGAAT 
CTCATTAATA AGATGGAAAG GGCGGGTCAT CTACATGAAA GTCATTTGGG AATGCTGCTG 
GACCGAGCTG AGGCTGAAAT TAATTCTGTT AAACAAAAAT TCAATCATGA TTTAAAACAA 
GAAAAGCAAA AGCTTCAGCA GAAACTCATT ACCAAGCGAA AGTGGAAAAT GTTGCAAAAG 
AAAGAGCATT GGAAGGAGCA GCTGTCCATA GGGAACCCCT TCAAAACCTC AAGGGAGATC 
ACTGACTACC TGAGCCACTG GAAAACTGTT CTGAGTGATC ATGCCATTGA ATTTGAAGAA 
CTTACTGAAA AGCTGGATAA TGAAGGCAGT GAAGAGCTGA GAGACCTTAT ATTTAATCTA 
GCAGAGAAAA CTGTTGAAGA ACTTAAACTT GTTCAATATG GAGTATTTGT GCAAGAACTG 
ATAAAGCTCA GTGTGCCAAA GATGTTCCTG CTGGAAGTTG TAGAGGAGCA TAAAAAAGAA 
TTCATTGTTA AACATGAACA CCTTGAAAGG GAAGAACGTG AAAAGAGCAT GGCTGCTGAA 
GAGTTGCTTC AGCTCACCAG GCAAAAACTA AGCCAAGAGC TGGAAAGTAG TCTTTCACAA 
CAGAAAAAAT TAAGGAACTG GGAACAATCA GTATTCATGC AGCTTCTAAC TTTGCCCCTT 
TCACTGTCTG AAGAGGAATT GCTCAAAATG AGGCAAGAGT TCCACTGCTG CTTTTCACAG 
CTGGACAGCA GTTTGGCTCT GCCCAAGATA AGAGCAAGAG CTTTACTTCA GGCTTTTGAG 
CTGGAGTGGA GGGATGCGCA GCTGCTGAAA GTCAGTCAGA ATCTAGCAAT GACCAATAAG 
CAGCTGCATT CTAAAACGAA AAAAACAGGA TCCAAGAATC GAAATAAGAT TGATGTTTTG 
AAGAAATCTT TACAGGACAA AATTTTCATT TATGAAGACT CAGTGAAAGT TGAAAACTTG 
ACTAAGGTCA ATTATGAGTT GCTACTTGAG AAAGACTGTC AGATACATGA TCTGGAAAAT 
AAACTTGGAG AATATATTGC TTCTTTAGAG TTTCAAAAGA GTGTTAAAAA ATGCAAAATG 
TTGGAGCTCT ATGCAGCAGT CATAAGTGTG CAAGGTTTGC TCTTGGAAAA CCTGAGCATG 
TCAAAAACGC TGTCAAAATC GGAATGTATT CAGATTTTAG AAGCTCATAA TCCGGAGATT 
GAAGAACTTG ACAGGAAGCT GGAGTATGAA ATGCTGCATA AAGAGTCAGC ACAACAGCAG 
CAACATCTAA TGAATAAGCA GAGGTGGACA CCGGATGGAT TGAGACCTTC AAGTGAAGTC 
GTAGAAACAA ATGTAGACAG ACAGGTGACA TTTCTGCTAA GACAGGCCAT GAATAGATGT 
ATGCAGTTTA TAAATCTGCA CCGGCAAAGC TTGAGAGATG AGCAATGGAA TTGTATGGTG 
CTTGAAGATC TCTTGGAAAA TATAGAAATG GATGCATTTT TGGCACTTTA TAGTCAGGAG 
CTCCGACTGG CAGGTTATTT AGCCAGATTG ACAAGGGTAC CAATGGGGAT GTTGCACAGG 
ATTCTGAATT TGTTGCTGCC TTCCAGTTCG CAAAGTGAGG TTCTTTCTAT CCTTGATTCC 
ATTGGTGTAT ATTCAGATGG TGCTGCTGAG AGTGATAGTA ATGCAGATGA GTCTGGCAGC 
TGTAAAAAAA GGAGGAACCA AGAGTGTTGG CAAGCATTGG AGTACAAAGT AAGGGAAGAT 
CTGGTAACCA AAAGTTTGGA AAGGATCCTT TCTCTAAATA GGAGAAAAGA CAGCTTGATA 
AAGAAGAAGC AACTTGCCCT TCTGGAAAGA CCATCATTCG TTCACCTTGA AAGTTTGCCT 
ACACGTCCTC CTGTGGAACG GTCCAGTCCA CATGCTTCTT TTAACAGTGT GACAGCTGAA 
GCCATCGAGG TATTAGACAC AGGGGAAAAG ATATTTTTGT TTCGTGACCG GAGTGATCCA 
ACACTTTCTC TTCATAACTA TCCGAGAAAA AAGAAGAAGA ACTTTCTTAA TTCCAAAAAG 
GCTGCTCGTG CAAATATCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015141281.1
CDS	185..3448
Translation	MFKKIVVMRQEMGCKFTGKDSCKLGNILQSTILHFLMQRKRGKNQTDLVSLTTLFTITEEEKTEVSYSHAIHASGFFIAFFVSLVLTCAVFFILYRTQLLRWSFYSENQEIQHELSQNHKLEHSQFSSGDLFSEDVIMNDQIIDILTFEESGNMLQALEDLEVASLTRADADLEAYRMQICKEVIAIMMKNMVLSHSLIPHVEKRMSSIFKKQFLTMEREFQEEYERKVVALTAECNLETRKKMEAQRQKERAANEEAEELIRKVNEMPAVEYKSLLDRLHSLEQDHLKRFLLVKQEEYFAKAYRQLAVSRRKELHNIFFTQITNATFKGELKLEIAKSLVEDYSKIQGDIEELMDFLQASKKYHLNRRFAYREYLIKKIQLRDSQASALINAAATQILNLINKMERAGHLHESHLGMLLDRAEAEINSVKQKFNHDLKQEKQKLQQKLITKRKWKMLQKKEHWKEQLSIGNPFKTSREITDYLSHWKTVLSDHAIEFEELTEKLDNEGSEELRDLIFNLAEKTVEELKLVQYGVFVQELIKLSVPKMFLLEVVEEHKKEFIVKHEHLEREEREKSMAAEELLQLTRQKLSQELESSLSQQKKLRNWEQSVFMQLLTLPLSLSEEELLKMRQEFHCCFSQLDSSLALPKIRARALLQAFELEWRDAQLLKVSQNLAMTNKQLHSKTKKTGSKNRNKIDVLKKSLQDKIFIYEDSVKVENLTKVNYELLLEKDCQIHDLENKLGEYIASLEFQKSVKKCKMLELYAAVISVQGLLLENLSMSKTLSKSECIQILEAHNPEIEELDRKLEYEMLHKESAQQQQHLMNKQRWTPDGLRPSSEVVETNVDRQVTFLLRQAMNRCMQFINLHRQSLRDEQWNCMVLEDLLENIEMDAFLALYSQELRLAGYLARLTRVPMGMLHRILNLLLPSSSQSEVLSILDSIGVYSDGAAESDSNADESGSCKKRRNQECWQALEYKVREDLVTKSLERILSLNRRKDSLIKKKQLALLERPSFVHLESLPTRPPVERSSPHASFNSVTAEAIEVLDTGEKIFLFRDRSDPTLSLHNYPRKKKKNFLNSKKAARANIN

Target ORF information:

RefSeq Version	XM_015285795.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus EvC ciliary complex subunit 2 (EVC2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285795.2

ORF Insert Sequence:

ATGTTCAAAA AGATAGTGGT AATGAGACAA GAGATGGGAT GCAAATTTAC AGGAAAAGAT 
TCTTGCAAGT TGGGGAATAT TTTGCAATCA ACTATACTGC ACTTCTTGAT GCAAAGAAAA 
CGTGGAAAGA ATCAAACAGA TCTTGTATCA TTGACCACAT TGTTCACAAT AACTGAAGAA 
GAAAAGACCG AGGTCTCCTA CAGCCATGCC ATCCATGCTT CAGGGTTCTT CATTGCTTTT 
TTTGTTTCCC TGGTATTGAC ATGTGCTGTT TTCTTCATTC TTTACCGAAC CCAATTATTA 
AGATGGAGTT TCTATAGTGA AAACCAGGAA ATACAACATG AACTCAGTCA AAATCACAAA 
CTGGAGCATT CTCAGTTCAG TTCAGGTGAC CTCTTCAGTG AAGATGTTAT TATGAATGAC 
CAAATCATTG ATATACTGAC CTTTGAAGAG TCGGGGAATA TGCTTCAGGC TTTAGAAGAC 
TTGGAGGTTG CCAGTCTGAC TCGGGCAGAT GCTGATTTGG AGGCATATCG AATGCAGATC 
TGTAAAGAAG TGATTGCGAT AATGATGAAG AACATGGTTT TAAGTCACAG CCTCATTCCG 
CATGTGGAGA AGAGGATGAG TTCAATTTTC AAAAAGCAGT TTCTTACAAT GGAGAGAGAG 
TTTCAGGAAG AGTACGAAAG GAAGGTTGTG GCTTTAACAG CTGAATGTAA TCTGGAAACA 
AGGAAGAAGA TGGAGGCTCA ACGCCAGAAA GAGAGAGCTG CAAATGAAGA GGCTGAAGAG 
TTAATAAGGA AAGTGAATGA AATGCCTGCT GTAGAATACA AAAGTCTTTT GGATAGACTT 
CACAGTCTGG AGCAGGACCA TCTGAAGAGG TTCCTTCTGG TAAAGCAAGA GGAGTATTTT 
GCAAAGGCTT ACAGACAACT GGCTGTTTCC CGGAGAAAGG AGCTCCATAA CATCTTCTTT 
ACACAGATAA CAAATGCTAC TTTCAAGGGA GAACTGAAAC TGGAAATAGC AAAATCATTA 
GTGGAAGATT ACTCTAAAAT ACAGGGAGAC ATTGAAGAGC TAATGGATTT TTTGCAAGCC 
AGCAAGAAGT ATCACCTGAA CAGAAGATTT GCTTACAGAG AATATCTTAT AAAAAAGATA 
CAGTTAAGGG ATTCTCAGGC CTCTGCTCTT ATAAATGCAG CAGCAACTCA GATATTGAAT 
CTCATTAATA AGATGGAAAG GGCGGGTCAT CTACATGAAA GTCATTTGGG AATGCTGCTG 
GACCGAGCTG AGGCTGAAAT TAATTCTGTT AAACAAAAAT TCAATCATGA TTTAAAACAA 
GAAAAGCAAA AGCTTCAGCA GAAACTCATT ACCAAGCGAA AGTGGAAAAT GTTGCAAAAG 
AAAGAGCATT GGAAGGAGCA GCTGTCCATA GGGAACCCCT TCAAAACCTC AAGGGAGATC 
ACTGACTACC TGAGCCACTG GAAAACTGTT CTGAGTGATC ATGCCATTGA ATTTGAAGAA 
CTTACTGAAA AGCTGGATAA TGAAGGCAGT GAAGAGCTGA GAGACCTTAT ATTTAATCTA 
GCAGAGAAAA CTGTTGAAGA ACTTAAACTT GTTCAATATG GAGTATTTGT GCAAGAACTG 
ATAAAGCTCA GTGTGCCAAA GATGTTCCTG CTGGAAGTTG TAGAGGAGCA TAAAAAAGAA 
TTCATTGTTA AACATGAACA CCTTGAAAGG GAAGAACGTG AAAAGAGCAT GGCTGCTGAA 
GAGTTGCTTC AGCTCACCAG GCAAAAACTA AGCCAAGAGC TGGAAAGTAG TCTTTCACAA 
CAGAAAAAAT TAAGGAACTG GGAACAATCA GTATTCATGC AGCTTCTAAC TTTGCCCCTT 
TCACTGTCTG AAGAGGAATT GCTCAAAATG AGGCAAGAGT TCCACTGCTG CTTTTCACAG 
CTGGACAGCA GTTTGGCTCT GCCCAAGATA AGAGCAAGAG CTTTACTTCA GGCTTTTGAG 
CTGGAGTGGA GGGATGCGCA GCTGCTGAAA GTCAGTCAGA ATCTAGCAAT GACCAATAAG 
CAGCTGCATT CTAAAACGAA AAAAACAGGA TCCAAGAATC GAAATAAGAT TGATGTTTTG 
AAGAAATCTT TACAGGACAA AATTTTCATT TATGAAGACT CAGTGAAAGT TGAAAACTTG 
ACTAAGGTCA ATTATGAGTT GCTACTTGAG AAAGACTGTC AGATACATGA TCTGGAAAAT 
AAACTTGGAG AATATATTGC TTCTTTAGAG TTTCAAAAGA GTGTTAAAAA ATGCAAAATG 
TTGGAGCTCT ATGCAGCAGT CATAAGTGTG CAAGGTTTGC TCTTGGAAAA CCTGAGCATG 
TCAAAAACGC TGTCAAAATC GGAATGTATT CAGATTTTAG AAGCTCATAA TCCGGAGATT 
GAAGAACTTG ACAGGAAGCT GGAGTATGAA ATGCTGCATA AAGAGTCAGC ACAACAGCAG 
CAACATCTAA TGAATAAGCA GAGGTGGACA CCGGATGGAT TGAGACCTTC AAGTGAAGTC 
GTAGAAACAA ATGTAGACAG ACAGGTGACA TTTCTGCTAA GACAGGCCAT GAATAGATGT 
ATGCAGTTTA TAAATCTGCA CCGGCAAAGC TTGAGAGATG AGCAATGGAA TTGTATGGTG 
CTTGAAGATC TCTTGGAAAA TATAGAAATG GATGCATTTT TGGCACTTTA TAGTCAGGAG 
CTCCGACTGG CAGGTTATTT AGCCAGATTG ACAAGGGTAC CAATGGGGAT GTTGCACAGG 
ATTCTGAATT TGTTGCTGCC TTCCAGTTCG CAAAGTGAGG TTCTTTCTAT CCTTGATTCC 
ATTGGTGTAT ATTCAGATGG TGCTGCTGAG AGTGATAGTA ATGCAGATGA GTCTGGCAGC 
TGTAAAAAAA GGAGGAACCA AGAGTGTTGG CAAGCATTGG AGTACAAAGT AAGGGAAGAT 
CTGGTAACCA AAAGTTTGGA AAGGATCCTT TCTCTAAATA GGAGAAAAGA CAGCTTGATA 
AAGAAGAAGC AACTTGCCCT TCTGGAAAGA CCATCATTCG TTCACCTTGA AAGTTTGCCT 
ACACGTCCTC CTGTGGAACG GTCCAGTCCA CATGCTTCTT TTAACAGTGT GACAGCTGAA 
GCCATCGAGG TATTAGACAC AGGGGAAAAG ATATTTTTGT TTCGTGACCG GAGTGATCCA 
ACACTTTCTC TTCATAACTA TCCGAGAAAA AAGAAGAAGA ACTTTCTTAA TTCCAAAAAG 
GCTGCTCGTG CAAATATCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EVC2 ( NM_001079749.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001073217.1 Gallus gallus EvC ciliary complex subunit 2 (EVC2), mRNA.

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CloneID	OGa24643
Clone ID Related Accession (Same CDS sequence)	NM_001079749.2
Accession Version	NM_001079749.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-20
Organism	Gallus gallus(chicken)
Product	limbin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY581318.1. On Apr 1, 2016 this sequence version replaced NM_001079749.1. ##Evidence-Data-START## Transcript exon combination :: AY581318.1 [ECO:0000332] ##Evidence-Data-END##

ATGGATGCGA AGGCTGAGGC CCGGTCTGGA TCGGGGCAGC GCGGCGCTCC GCGGGCCGGC 
CCGGTGCCGT GCGGGGCTGG GCGGCTCCTG GCCGGGCTGC TCGTGGTACT GCTGTCCTCC 
GCGCCCGCTC AGCCCCTCGG GGCGGCCGCC TGGTGCCCCA CGCAGGGACA CGGGGTGCCG 
GGCCCACCCC GGGGGCACTG CGGGAGGACG GCGGCTGGGG TTATTACAGA TGATCCGTGG 
CTTCAAAAAG AGATGAATTG TCTGCGGATG ACAGCACTGC AGTTGCCTGT GGTGGTTCAA 
GCAAGTGAAG TCCCTGATCT GATCTTGACT TCTGCAGCCT CGGAAGATCA GTGGAGCCAT 
TCGATATTTG CTCTGATATC CTCATGGACA AGGAAGATTT TGTTCAAACG AGAGTCTTCT 
GTTAACCACC AGCTGCCTGA AGATGTGTCC AACTTGTCAG CATCCTCTGG ATTTGGAATA 
ACCCTTCAGA AATGTGCTGT GGTGGATGTT GAACATGACC CTCAAACTGC ATACATTAGA 
CTTCTGATTA ATAACACCAA CTCACTGCTC TCAAGAAACA TTACTGATCT TATCCTTCTG 
GACAATATCA CCGGTCTACA TGTTCAAAAA GATAGTGGTA ATGAGACAAG AGATGGGATG 
CAAATTTACA GGAAAAGATT CTTGCAAGTT GGGGAATATT TTGCAATCAA CTACACTGCA 
CTTCTTGATG CAAAGAAAAC GTGGAAAGGT AGGGTCTTGA CACTGCCTGC ACAGCTGACT 
TTCAGGAGTT CATCACAGAA TCAAACAGAT CTTGTATCAT TGACCACATT GTTCACAATA 
ACTGAAGAAG AAAAGACCGA GGTCTCCTAC AGCCATGCCA TCCATGCTTC AGGGTTCTTC 
ATTGCTTTTT TTGTTTCCCT GGTATTGACA TGTGCTGTTT TCTTCATTCT TTACCGAACC 
CAATTATTAA GATGGAGTTT CTATAGTGAA AACCAGGAAA TACAACATGA ACTCAGTCAA 
AATCACAAAC TGGAGCATTC TCAGTTCAGT TCAGGTGACC TCTTCAGTGA AGATGTTATT 
ATGAATGACC AAATCATTGA TATACTGACC TTTGAAGAGT CGGGGAATAT GCTTCAGGCT 
TTAGAAGACT TGGAGGTTGC CAGTCTGACT CGGGCAGATG CTGATTTGGA GGCATATCGA 
ATGCAGATCT GTAAAGAAGT GATTGCTATA ATGATGAAGA ACATGGTTTT AAGTCACAGC 
CTCATTCCGC ATGTGGAGAA GAGGATGAGT TCAATTTTCA AAAAGCAGTT TCTTACAATG 
GAGAGAGAGT TTCAGGAAGA GTACGAAAGG AAGGTTGTGG CTTTAACAGC TGAATGTAAT 
CTGGAAACAA GGAAGAAGAT GGAGGCTCAA CGCCAGAAAG AGAGAGCTGC AAATGAAGAG 
GCTGAAGAGT TAATAAGGAA AGTGAATGAA ATGCCTGCTG TAGAATACAA AAGTCTTTTG 
GATAGACTTC ACAGTCTGGA GCAGGACCAT CTGAAGAGGT TCCTTCTTGT AAAGCAAGAG 
GAATATTTTG CAAAGGCTTA CAGACAGCTG GCTGTTTCCC AGAGAAAAGA GCTCCATAAC 
ATCTTCTTTA CACAGATAAC AAATGCTACT TTCAAGGGAG AACTGAAACT GGAAATAGCA 
AAATCATTAG TGGAAGATTA CTCTAAAATA CAGGGAGACA TTGAAGAGCT AATGGATTTT 
TTGCAAGCCA GCAAGAAGTA TCACCTGAAC AGAAGATTTG CTTACAGAGA ATATCTTATA 
AAAAAGATAC AGTTAAGGGA TTCTCAGGCC TCTGCTCTTA TAAATGCAGC AGCAACTCAG 
ATATTGAATC TCATTAATAA GATGGAAAGG GCGGGTCATC TACATGAAAG TCATTTGGGA 
ATGCTGCTGG ACCGAGCTGA GGCTGAAATT AATTCTGTTA AACAAAAATT CAATCATGAT 
TTAAAACAAG AAAAGCAAAA GCTTCAGCAG AAACTCATTA CCAAGCGAAA GTGGAAAATG 
TTGCAAAAGA AAGAGCATTG GAAGGAGCAG CTGTCCATAG GGAACCCCTT CAAAACCTCA 
AGGGAGATCA CTGACTACCT GAGCCACTGG AAAACTGTTC TGAGTGATCA TGCCATTGAA 
TTTGAAGAAC TTACTGAAAA GCTGGATAAT GAAGGCAGTG AAGAGCTGAG AGACCTTATA 
TTTAATCTAG CAGAGAAAAC TGTTGAAGAA CTTAAACTTG TTCAATATGG AGTATTTGTG 
CAAGAACTGA TAAAGCTCAG TGTGCCAAAG ATGTTCCTGC TGGAAGTTGT AGAGGAGCAT 
AAAAAAGAAT TCATTGTTAA ACGTGAACAC CTTGAAAGGG AAGAACGTGA AAAGATCATG 
GCTGCTGAAG AGTTGCTTCA GCTCACCAGG CAAAAACTAA GCCAAGAGCT GGAAAGTAGT 
CTTTCACAAC AGAAAAAATT AAGGAACTGG GAACAATCAG TATTCATGCA GCTTCTAACT 
TTGCCCCTTT CACTGTCTGA AGAGGAATTG CTCAAAATGA GGCAAGAGTT CCACTGCTGC 
TTTTCACAGC TGGACAGCAG TTTGGCTCTG CCCAAGATAA GAGCAAGAGC TTTACTTCAG 
GCTTTTGAGC TGGAGTGGAG GGATGCGCAG CTGCTGAAAG TCAGTCAGAA TCTAGCAATG 
ACCAATAAGC AGCTGCATTC TAAAACGAAA AAAACAGGAT CCAAGAATCG AAATAAGATT 
GATGTTTTGA AGAAATCTTT ACAGGACAAA ATTTTCATTT ATGAAGACTC AGTGAAAGTT 
GAAAACTTGA CTAAGGTCAA TTATGAGTTG CTACTTGAGA AAGACTGTCA GATACATGAT 
CTGGAAAATA AACTTGGAGA ATATATTGCT TCTTTAGAGT TTCAAAAGAG TGTTAAAAAA 
TGCAAAATGT TGGAGCTCTA TGCAGCAGTC ATAAGTGTGC AAGGTTTGCT CTTGGAAAAC 
CTGAGCATGT CAAAAACGCT GTCAAAATCG GAATGTATTC AGATTTTAGA AGCTCATAAT 
CCGGAGATTG AAGAACTTGA CAGGAAGCTG GAGTATGAAA TGCTGCATAA AGAGTCAGCA 
CAACAGCAGC AACATCTAAT GAATAAGCAG AGGTGGACAC CGGATGGATT GAGACCTTCA 
AGTGAAGTCG TAGAAACAAA TGTAGACAGA CAGGTGACAT TTCTGCTAAG ACAGGCCATG 
AATAGATGTA TGCAGTTTAT AAATCTGCAC CGGCAAAGCT TGAGAGATGA GCAATGGAAT 
TGTATGGTGC TTGAAGATCT CTTGGAAAAT ATAGAAATGG ATGCATTTTT GGCACTTTAT 
AGTCAGGAGC TCCGACTGGC AGGTTATTTA GCCAGATTGA CAAGGGTACC AATGGGGATG 
TTGCACAGGA TTCTGAATTT GTTGCTGCCT TCCAGTTCGC AAAGTGAGGT TCTTTCTATC 
CTTGATTCCA TTGGTGTATA TTCAGATGGT GCTGCTGAGA GTGATAGTAA TGCAGATGAG 
TCTGGCAGCT GTAAAAAAAG GAGGAACCAA GAGTGTTGGC AAGCATTGGA GTACAAAGTA 
AGGGAAGATC TGGTAACCAA AAGTTTGGAA AGGATCCTTT CTCTAAATAG GAGAAAAGAC 
AGCTTGATAA AGAAGAAGCA ACTTGCCCTT CTGGAAAGAC CATCATTCGT TCACCTTGAA 
AGTTTGCCTA CACGTCCTCC TGTGGAACGG TCCAGTCCAC ATGTTTCTTT TAACAGTGTG 
ACAGCTGAAG CCATCGAGCT ATTAGACACA GGGGAAAAGA TATTTTTGTT TCGTGACCGG 
AGTGATCCAA CACTTTCTTT TCATAACTAT CCGAGAAAAA AGAAGAAGAA CTTTCTTAAT 
TCCAAAAAGG CTGCTCGTGC AAATATCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073217.1
CDS	16..3948
Translation	MDAKAEARSGSGQRGAPRAGPVPCGAGRLLAGLLVVLLSSAPAQPLGAAAWCPTQGHGVPGPPRGHCGRTAAGVITDDPWLQKEMNCLRMTALQLPVVVQASEVPDLILTSAASEDQWSHSIFALISSWTRKILFKRESSVNHQLPEDVSNLSASSGFGITLQKCAVVDVEHDPQTAYIRLLINNTNSLLSRNITDLILLDNITGLHVQKDSGNETRDGMQIYRKRFLQVGEYFAINYTALLDAKKTWKGRVLTLPAQLTFRSSSQNQTDLVSLTTLFTITEEEKTEVSYSHAIHASGFFIAFFVSLVLTCAVFFILYRTQLLRWSFYSENQEIQHELSQNHKLEHSQFSSGDLFSEDVIMNDQIIDILTFEESGNMLQALEDLEVASLTRADADLEAYRMQICKEVIAIMMKNMVLSHSLIPHVEKRMSSIFKKQFLTMEREFQEEYERKVVALTAECNLETRKKMEAQRQKERAANEEAEELIRKVNEMPAVEYKSLLDRLHSLEQDHLKRFLLVKQEEYFAKAYRQLAVSQRKELHNIFFTQITNATFKGELKLEIAKSLVEDYSKIQGDIEELMDFLQASKKYHLNRRFAYREYLIKKIQLRDSQASALINAAATQILNLINKMERAGHLHESHLGMLLDRAEAEINSVKQKFNHDLKQEKQKLQQKLITKRKWKMLQKKEHWKEQLSIGNPFKTSREITDYLSHWKTVLSDHAIEFEELTEKLDNEGSEELRDLIFNLAEKTVEELKLVQYGVFVQELIKLSVPKMFLLEVVEEHKKEFIVKREHLEREEREKIMAAEELLQLTRQKLSQELESSLSQQKKLRNWEQSVFMQLLTLPLSLSEEELLKMRQEFHCCFSQLDSSLALPKIRARALLQAFELEWRDAQLLKVSQNLAMTNKQLHSKTKKTGSKNRNKIDVLKKSLQDKIFIYEDSVKVENLTKVNYELLLEKDCQIHDLENKLGEYIASLEFQKSVKKCKMLELYAAVISVQGLLLENLSMSKTLSKSECIQILEAHNPEIEELDRKLEYEMLHKESAQQQQHLMNKQRWTPDGLRPSSEVVETNVDRQVTFLLRQAMNRCMQFINLHRQSLRDEQWNCMVLEDLLENIEMDAFLALYSQELRLAGYLARLTRVPMGMLHRILNLLLPSSSQSEVLSILDSIGVYSDGAAESDSNADESGSCKKRRNQECWQALEYKVREDLVTKSLERILSLNRRKDSLIKKKQLALLERPSFVHLESLPTRPPVERSSPHVSFNSVTAEAIELLDTGEKIFLFRDRSDPTLSFHNYPRKKKKNFLNSKKAARANIN

Target ORF information:

RefSeq Version	NM_001079749.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus EvC ciliary complex subunit 2 (EVC2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079749.2

ORF Insert Sequence:

ATGGATGCGA AGGCTGAGGC CCGGTCTGGA TCGGGGCAGC GCGGCGCTCC GCGGGCCGGC 
CCGGTGCCGT GCGGGGCTGG GCGGCTCCTG GCCGGGCTGC TCGTGGTACT GCTGTCCTCC 
GCGCCCGCTC AGCCCCTCGG GGCGGCCGCC TGGTGCCCCA CGCAGGGACA CGGGGTGCCG 
GGCCCACCCC GGGGGCACTG CGGGAGGACG GCGGCTGGGG TTATTACAGA TGATCCGTGG 
CTTCAAAAAG AGATGAATTG TCTGCGGATG ACAGCACTGC AGTTGCCTGT GGTGGTTCAA 
GCAAGTGAAG TCCCTGATCT GATCTTGACT TCTGCAGCCT CGGAAGATCA GTGGAGCCAT 
TCGATATTTG CTCTGATATC CTCATGGACA AGGAAGATTT TGTTCAAACG AGAGTCTTCT 
GTTAACCACC AGCTGCCTGA AGATGTGTCC AACTTGTCAG CATCCTCTGG ATTTGGAATA 
ACCCTTCAGA AATGTGCTGT GGTGGATGTT GAACATGACC CTCAAACTGC ATACATTAGA 
CTTCTGATTA ATAACACCAA CTCACTGCTC TCAAGAAACA TTACTGATCT TATCCTTCTG 
GACAATATCA CCGGTCTACA TGTTCAAAAA GATAGTGGTA ATGAGACAAG AGATGGGATG 
CAAATTTACA GGAAAAGATT CTTGCAAGTT GGGGAATATT TTGCAATCAA CTACACTGCA 
CTTCTTGATG CAAAGAAAAC GTGGAAAGGT AGGGTCTTGA CACTGCCTGC ACAGCTGACT 
TTCAGGAGTT CATCACAGAA TCAAACAGAT CTTGTATCAT TGACCACATT GTTCACAATA 
ACTGAAGAAG AAAAGACCGA GGTCTCCTAC AGCCATGCCA TCCATGCTTC AGGGTTCTTC 
ATTGCTTTTT TTGTTTCCCT GGTATTGACA TGTGCTGTTT TCTTCATTCT TTACCGAACC 
CAATTATTAA GATGGAGTTT CTATAGTGAA AACCAGGAAA TACAACATGA ACTCAGTCAA 
AATCACAAAC TGGAGCATTC TCAGTTCAGT TCAGGTGACC TCTTCAGTGA AGATGTTATT 
ATGAATGACC AAATCATTGA TATACTGACC TTTGAAGAGT CGGGGAATAT GCTTCAGGCT 
TTAGAAGACT TGGAGGTTGC CAGTCTGACT CGGGCAGATG CTGATTTGGA GGCATATCGA 
ATGCAGATCT GTAAAGAAGT GATTGCTATA ATGATGAAGA ACATGGTTTT AAGTCACAGC 
CTCATTCCGC ATGTGGAGAA GAGGATGAGT TCAATTTTCA AAAAGCAGTT TCTTACAATG 
GAGAGAGAGT TTCAGGAAGA GTACGAAAGG AAGGTTGTGG CTTTAACAGC TGAATGTAAT 
CTGGAAACAA GGAAGAAGAT GGAGGCTCAA CGCCAGAAAG AGAGAGCTGC AAATGAAGAG 
GCTGAAGAGT TAATAAGGAA AGTGAATGAA ATGCCTGCTG TAGAATACAA AAGTCTTTTG 
GATAGACTTC ACAGTCTGGA GCAGGACCAT CTGAAGAGGT TCCTTCTTGT AAAGCAAGAG 
GAATATTTTG CAAAGGCTTA CAGACAGCTG GCTGTTTCCC AGAGAAAAGA GCTCCATAAC 
ATCTTCTTTA CACAGATAAC AAATGCTACT TTCAAGGGAG AACTGAAACT GGAAATAGCA 
AAATCATTAG TGGAAGATTA CTCTAAAATA CAGGGAGACA TTGAAGAGCT AATGGATTTT 
TTGCAAGCCA GCAAGAAGTA TCACCTGAAC AGAAGATTTG CTTACAGAGA ATATCTTATA 
AAAAAGATAC AGTTAAGGGA TTCTCAGGCC TCTGCTCTTA TAAATGCAGC AGCAACTCAG 
ATATTGAATC TCATTAATAA GATGGAAAGG GCGGGTCATC TACATGAAAG TCATTTGGGA 
ATGCTGCTGG ACCGAGCTGA GGCTGAAATT AATTCTGTTA AACAAAAATT CAATCATGAT 
TTAAAACAAG AAAAGCAAAA GCTTCAGCAG AAACTCATTA CCAAGCGAAA GTGGAAAATG 
TTGCAAAAGA AAGAGCATTG GAAGGAGCAG CTGTCCATAG GGAACCCCTT CAAAACCTCA 
AGGGAGATCA CTGACTACCT GAGCCACTGG AAAACTGTTC TGAGTGATCA TGCCATTGAA 
TTTGAAGAAC TTACTGAAAA GCTGGATAAT GAAGGCAGTG AAGAGCTGAG AGACCTTATA 
TTTAATCTAG CAGAGAAAAC TGTTGAAGAA CTTAAACTTG TTCAATATGG AGTATTTGTG 
CAAGAACTGA TAAAGCTCAG TGTGCCAAAG ATGTTCCTGC TGGAAGTTGT AGAGGAGCAT 
AAAAAAGAAT TCATTGTTAA ACGTGAACAC CTTGAAAGGG AAGAACGTGA AAAGATCATG 
GCTGCTGAAG AGTTGCTTCA GCTCACCAGG CAAAAACTAA GCCAAGAGCT GGAAAGTAGT 
CTTTCACAAC AGAAAAAATT AAGGAACTGG GAACAATCAG TATTCATGCA GCTTCTAACT 
TTGCCCCTTT CACTGTCTGA AGAGGAATTG CTCAAAATGA GGCAAGAGTT CCACTGCTGC 
TTTTCACAGC TGGACAGCAG TTTGGCTCTG CCCAAGATAA GAGCAAGAGC TTTACTTCAG 
GCTTTTGAGC TGGAGTGGAG GGATGCGCAG CTGCTGAAAG TCAGTCAGAA TCTAGCAATG 
ACCAATAAGC AGCTGCATTC TAAAACGAAA AAAACAGGAT CCAAGAATCG AAATAAGATT 
GATGTTTTGA AGAAATCTTT ACAGGACAAA ATTTTCATTT ATGAAGACTC AGTGAAAGTT 
GAAAACTTGA CTAAGGTCAA TTATGAGTTG CTACTTGAGA AAGACTGTCA GATACATGAT 
CTGGAAAATA AACTTGGAGA ATATATTGCT TCTTTAGAGT TTCAAAAGAG TGTTAAAAAA 
TGCAAAATGT TGGAGCTCTA TGCAGCAGTC ATAAGTGTGC AAGGTTTGCT CTTGGAAAAC 
CTGAGCATGT CAAAAACGCT GTCAAAATCG GAATGTATTC AGATTTTAGA AGCTCATAAT 
CCGGAGATTG AAGAACTTGA CAGGAAGCTG GAGTATGAAA TGCTGCATAA AGAGTCAGCA 
CAACAGCAGC AACATCTAAT GAATAAGCAG AGGTGGACAC CGGATGGATT GAGACCTTCA 
AGTGAAGTCG TAGAAACAAA TGTAGACAGA CAGGTGACAT TTCTGCTAAG ACAGGCCATG 
AATAGATGTA TGCAGTTTAT AAATCTGCAC CGGCAAAGCT TGAGAGATGA GCAATGGAAT 
TGTATGGTGC TTGAAGATCT CTTGGAAAAT ATAGAAATGG ATGCATTTTT GGCACTTTAT 
AGTCAGGAGC TCCGACTGGC AGGTTATTTA GCCAGATTGA CAAGGGTACC AATGGGGATG 
TTGCACAGGA TTCTGAATTT GTTGCTGCCT TCCAGTTCGC AAAGTGAGGT TCTTTCTATC 
CTTGATTCCA TTGGTGTATA TTCAGATGGT GCTGCTGAGA GTGATAGTAA TGCAGATGAG 
TCTGGCAGCT GTAAAAAAAG GAGGAACCAA GAGTGTTGGC AAGCATTGGA GTACAAAGTA 
AGGGAAGATC TGGTAACCAA AAGTTTGGAA AGGATCCTTT CTCTAAATAG GAGAAAAGAC 
AGCTTGATAA AGAAGAAGCA ACTTGCCCTT CTGGAAAGAC CATCATTCGT TCACCTTGAA 
AGTTTGCCTA CACGTCCTCC TGTGGAACGG TCCAGTCCAC ATGTTTCTTT TAACAGTGTG 
ACAGCTGAAG CCATCGAGCT ATTAGACACA GGGGAAAAGA TATTTTTGTT TCGTGACCGG 
AGTGATCCAA CACTTTCTTT TCATAACTAT CCGAGAAAAA AGAAGAAGAA CTTTCTTAAT 
TCCAAAAAGG CTGCTCGTGC AAATATCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EVC2 ( XM_015285793.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015141279.1 Gallus gallus Ellis van Creveld syndrome 2 (EVC2), transcript variant X1, mRNA.

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CloneID	OGa35733
Clone ID Related Accession (Same CDS sequence)	XM_015285793.1 , XM_015285793.2
Accession Version	XM_015285793.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	limbin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455863.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCGA AGGCTGAGGC CCGGTCTGGA TCGGGGCAGC GCGGCGCTCC GCGGGCCGGC 
CCGGTGCCGT GCGGGGCTGG GCGGCTCCTG GCCGGGCTGC TCGTGGTACT GCTGTCCTCC 
GCGCCCGCTC AGCCCCTCGG GGCGGCTGCC TGGTGCCCCA CGCAGGGACA CGGGGTGCCG 
GGCCCACCCC GGGGGCACTG CGGGAGGACG GCGGCTGGGG TTATTACAGA TGATCCGTGG 
CTTCAAAAAG AGATGAATTG TCTGCGGATG ACAGCACTGC AGTTGCCTGT GGTGGTTCAA 
GCAAGTGAAG TCCCTGATCT GATCTTGACT TCTGCAGCCT CGGAAGATCA GTGGAGCCAT 
TCAATATTTG CTCTGATACC CTCATGGACA AGGAAGATTT TGTTCAAACG AGAGTCTTCT 
GTTAACCACC AGCTGTCTTT TCCCCTTCCA CCACAGCCTG AAGATGTGTC CAACTTGTCA 
GCATCCTCTG GATTTGGAAT AACCCTTCAG AAATGTGCTG TGGTGGATGT TGAACATGAC 
CCTCAAACTG CATACATTAG ACTTCTGATT AATAACACCA ACTCACTGCT CTCAAGAAAC 
ATTACTGATC TTATCCTTCT GGACAATATC ACCGGTCTAC ATGTTCAAAA AGATAGTGGT 
AATGAGACAA GAGATGGGAT GCAAATTTAC AGGAAAAGAT TCTTGCAAGT TGGGGAATAT 
TTTGCAATCA ACTATACTGC ACTTCTTGAT GCAAAGAAAA CGTGGAAAGG TAGGGTCTTG 
ACACTGCCTG CACAGCTGAC TTTCAGGAGT TCATCACAGA ATCAAACAGA TCTTGTATCA 
TTGACCACAT TGTTCACAAT AACTGAAGAA GAAAAGACCG AGGTCTCCTA CAGCCATGCC 
ATCCATGCTT CAGGGTTCTT CATTGCTTTT TTTGTTTCCC TGGTATTGAC ATGTGCTGTT 
TTCTTCATTC TTTACCGAAC CCAATTATTA AGATGGAGTT TCTATAGTGA AAACCAGGAA 
ATACAACATG AACTCAGTCA AAATCACAAA CTGGAGCATT CTCAGTTCAG TTCAGGTGAC 
CTCTTCAGTG AAGATGTTAT TATGAATGAC CAAATCATTG ATATACTGAC CTTTGAAGAG 
TCGGGGAATA TGCTTCAGGC TTTAGAAGAC TTGGAGGTTG CCAGTCTGAC TCGGGCAGAT 
GCTGATTTGG AGGCATATCG AATGCAGATC TGTAAAGAAG TGATTGCGAT AATGATGAAG 
AACATGGTTT TAAGTCACAG CCTCATTCCG CATGTGGAGA AGAGGATGAG TTCAATTTTC 
AAAAAGCAGT TTCTTACAAT GGAGAGAGAG TTTCAGGAAG AGTACGAAAG GAAGGTTGTG 
GCTTTAACAG CTGAATGTAA TCTGGAAACA AGGAAGAAGA TGGAGGCTCA ACGCCAGAAA 
GAGAGAGCTG CAAATGAAGA GGCTGAAGAG TTAATAAGGA AAGTGAATGA AATGCCTGCT 
GTAGAATACA AAAGTCTTTT GGATAGACTT CACAGTCTGG AGCAGGACCA TCTGAAGAGG 
TTCCTTCTGG TAAAGCAAGA GGAGTATTTT GCAAAGGCTT ACAGACAACT GGCTGTTTCC 
CGGAGAAAGG AGCTCCATAA CATCTTCTTT ACACAGATAA CAAATGCTAC TTTCAAGGGA 
GAACTGAAAC TGGAAATAGC AAAATCATTA GTGGAAGATT ACTCTAAAAT ACAGGGAGAC 
ATTGAAGAGC TAATGGATTT TTTGCAAGCC AGCAAGAAGT ATCACCTGAA CAGAAGATTT 
GCTTACAGAG AATATCTTAT AAAAAAGATA CAGTTAAGGG ATTCTCAGGC CTCTGCTCTT 
ATAAATGCAG CAGCAACTCA GATATTGAAT CTCATTAATA AGATGGAAAG GGCGGGTCAT 
CTACATGAAA GTCATTTGGG AATGCTGCTG GACCGAGCTG AGGCTGAAAT TAATTCTGTT 
AAACAAAAAT TCAATCATGA TTTAAAACAA GAAAAGCAAA AGCTTCAGCA GAAACTCATT 
ACCAAGCGAA AGTGGAAAAT GTTGCAAAAG AAAGAGCATT GGAAGGAGCA GCTGTCCATA 
GGGAACCCCT TCAAAACCTC AAGGGAGATC ACTGACTACC TGAGCCACTG GAAAACTGTT 
CTGAGTGATC ATGCCATTGA ATTTGAAGAA CTTACTGAAA AGCTGGATAA TGAAGGCAGT 
GAAGAGCTGA GAGACCTTAT ATTTAATCTA GCAGAGAAAA CTGTTGAAGA ACTTAAACTT 
GTTCAATATG GAGTATTTGT GCAAGAACTG ATAAAGCTCA GTGTGCCAAA GATGTTCCTG 
CTGGAAGTTG TAGAGGAGCA TAAAAAAGAA TTCATTGTTA AACATGAACA CCTTGAAAGG 
GAAGAACGTG AAAAGAGCAT GGCTGCTGAA GAGTTGCTTC AGCTCACCAG GCAAAAACTA 
AGCCAAGAGC TGGAAAGTAG TCTTTCACAA CAGAAAAAAT TAAGGAACTG GGAACAATCA 
GTATTCATGC AGCTTCTAAC TTTGCCCCTT TCACTGTCTG AAGAGGAATT GCTCAAAATG 
AGGCAAGAGT TCCACTGCTG CTTTTCACAG CTGGACAGCA GTTTGGCTCT GCCCAAGATA 
AGAGCAAGAG CTTTACTTCA GGCTTTTGAG CTGGAGTGGA GGGATGCGCA GCTGCTGAAA 
GTCAGTCAGA ATCTAGCAAT GACCAATAAG CAGCTGCATT CTAAAACGAA AAAAACAGGA 
TCCAAGAATC GAAATAAGAT TGATGTTTTG AAGAAATCTT TACAGGACAA AATTTTCATT 
TATGAAGACT CAGTGAAAGT TGAAAACTTG ACTAAGGTCA ATTATGAGTT GCTACTTGAG 
AAAGACTGTC AGATACATGA TCTGGAAAAT AAACTTGGAG AATATATTGC TTCTTTAGAG 
TTTCAAAAGA GTGTTAAAAA ATGCAAAATG TTGGAGCTCT ATGCAGCAGT CATAAGTGTG 
CAAGGTTTGC TCTTGGAAAA CCTGAGCATG TCAAAAACGC TGTCAAAATC GGAATGTATT 
CAGATTTTAG AAGCTCATAA TCCGGAGATT GAAGAACTTG ACAGGAAGCT GGAGTATGAA 
ATGCTGCATA AAGAGTCAGC ACAACAGCAG CAACATCTAA TGAATAAGCA GAGGTGGACA 
CCGGATGGAT TGAGACCTTC AAGTGAAGTC GTAGAAACAA ATGTAGACAG ACAGGTGACA 
TTTCTGCTAA GACAGGCCAT GAATAGATGT ATGCAGTTTA TAAATCTGCA CCGGCAAAGC 
TTGAGAGATG AGCAATGGAA TTGTATGGTG CTTGAAGATC TCTTGGAAAA TATAGAAATG 
GATGCATTTT TGGCACTTTA TAGTCAGGAG CTCCGACTGG CAGGTTATTT AGCCAGATTG 
ACAAGGGTAC CAATGGGGAT GTTGCACAGG ATTCTGAATT TGTTGCTGCC TTCCAGTTCG 
CAAAGTGAGG TTCTTTCTAT CCTTGATTCC ATTGGTGTAT ATTCAGATGG TGCTGCTGAG 
AGTGATAGTA ATGCAGATGA GTCTGGCAGC TGTAAAAAAA GGAGGAACCA AGAGTGTTGG 
CAAGCATTGG AGTACAAAGT AAGGGAAGAT CTGGTAACCA AAAGTTTGGA AAGGATCCTT 
TCTCTAAATA GGAGAAAAGA CAGCTTGATA AAGAAGAAGC AACTTGCCCT TCTGGAAAGA 
CCATCATTCG TTCACCTTGA AAGTTTGCCT ACACGTCCTC CTGTGGAACG GTCCAGTCCA 
CATGCTTCTT TTAACAGTGT GACAGCTGAA GCCATCGAGG TATTAGACAC AGGGGAAAAG 
ATATTTTTGT TTCGTGACCG GAGTGATCCA ACACTTTCTC TTCATAACTA TCCGAGAAAA 
AAGAAGAAGA ACTTTCTTAA TTCCAAAAAG GCTGCTCGTG CAAATATCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015141279.1
CDS	24..3977
Translation	MDAKAEARSGSGQRGAPRAGPVPCGAGRLLAGLLVVLLSSAPAQPLGAAAWCPTQGHGVPGPPRGHCGRTAAGVITDDPWLQKEMNCLRMTALQLPVVVQASEVPDLILTSAASEDQWSHSIFALIPSWTRKILFKRESSVNHQLSFPLPPQPEDVSNLSASSGFGITLQKCAVVDVEHDPQTAYIRLLINNTNSLLSRNITDLILLDNITGLHVQKDSGNETRDGMQIYRKRFLQVGEYFAINYTALLDAKKTWKGRVLTLPAQLTFRSSSQNQTDLVSLTTLFTITEEEKTEVSYSHAIHASGFFIAFFVSLVLTCAVFFILYRTQLLRWSFYSENQEIQHELSQNHKLEHSQFSSGDLFSEDVIMNDQIIDILTFEESGNMLQALEDLEVASLTRADADLEAYRMQICKEVIAIMMKNMVLSHSLIPHVEKRMSSIFKKQFLTMEREFQEEYERKVVALTAECNLETRKKMEAQRQKERAANEEAEELIRKVNEMPAVEYKSLLDRLHSLEQDHLKRFLLVKQEEYFAKAYRQLAVSRRKELHNIFFTQITNATFKGELKLEIAKSLVEDYSKIQGDIEELMDFLQASKKYHLNRRFAYREYLIKKIQLRDSQASALINAAATQILNLINKMERAGHLHESHLGMLLDRAEAEINSVKQKFNHDLKQEKQKLQQKLITKRKWKMLQKKEHWKEQLSIGNPFKTSREITDYLSHWKTVLSDHAIEFEELTEKLDNEGSEELRDLIFNLAEKTVEELKLVQYGVFVQELIKLSVPKMFLLEVVEEHKKEFIVKHEHLEREEREKSMAAEELLQLTRQKLSQELESSLSQQKKLRNWEQSVFMQLLTLPLSLSEEELLKMRQEFHCCFSQLDSSLALPKIRARALLQAFELEWRDAQLLKVSQNLAMTNKQLHSKTKKTGSKNRNKIDVLKKSLQDKIFIYEDSVKVENLTKVNYELLLEKDCQIHDLENKLGEYIASLEFQKSVKKCKMLELYAAVISVQGLLLENLSMSKTLSKSECIQILEAHNPEIEELDRKLEYEMLHKESAQQQQHLMNKQRWTPDGLRPSSEVVETNVDRQVTFLLRQAMNRCMQFINLHRQSLRDEQWNCMVLEDLLENIEMDAFLALYSQELRLAGYLARLTRVPMGMLHRILNLLLPSSSQSEVLSILDSIGVYSDGAAESDSNADESGSCKKRRNQECWQALEYKVREDLVTKSLERILSLNRRKDSLIKKKQLALLERPSFVHLESLPTRPPVERSSPHASFNSVTAEAIEVLDTGEKIFLFRDRSDPTLSLHNYPRKKKKNFLNSKKAARANIN

Target ORF information:

RefSeq Version	XM_015285793.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ellis van Creveld syndrome 2 (EVC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285793.1

ORF Insert Sequence:

ATGGATGCGA AGGCTGAGGC CCGGTCTGGA TCGGGGCAGC GCGGCGCTCC GCGGGCCGGC 
CCGGTGCCGT GCGGGGCTGG GCGGCTCCTG GCCGGGCTGC TCGTGGTACT GCTGTCCTCC 
GCGCCCGCTC AGCCCCTCGG GGCGGCTGCC TGGTGCCCCA CGCAGGGACA CGGGGTGCCG 
GGCCCACCCC GGGGGCACTG CGGGAGGACG GCGGCTGGGG TTATTACAGA TGATCCGTGG 
CTTCAAAAAG AGATGAATTG TCTGCGGATG ACAGCACTGC AGTTGCCTGT GGTGGTTCAA 
GCAAGTGAAG TCCCTGATCT GATCTTGACT TCTGCAGCCT CGGAAGATCA GTGGAGCCAT 
TCAATATTTG CTCTGATACC CTCATGGACA AGGAAGATTT TGTTCAAACG AGAGTCTTCT 
GTTAACCACC AGCTGTCTTT TCCCCTTCCA CCACAGCCTG AAGATGTGTC CAACTTGTCA 
GCATCCTCTG GATTTGGAAT AACCCTTCAG AAATGTGCTG TGGTGGATGT TGAACATGAC 
CCTCAAACTG CATACATTAG ACTTCTGATT AATAACACCA ACTCACTGCT CTCAAGAAAC 
ATTACTGATC TTATCCTTCT GGACAATATC ACCGGTCTAC ATGTTCAAAA AGATAGTGGT 
AATGAGACAA GAGATGGGAT GCAAATTTAC AGGAAAAGAT TCTTGCAAGT TGGGGAATAT 
TTTGCAATCA ACTATACTGC ACTTCTTGAT GCAAAGAAAA CGTGGAAAGG TAGGGTCTTG 
ACACTGCCTG CACAGCTGAC TTTCAGGAGT TCATCACAGA ATCAAACAGA TCTTGTATCA 
TTGACCACAT TGTTCACAAT AACTGAAGAA GAAAAGACCG AGGTCTCCTA CAGCCATGCC 
ATCCATGCTT CAGGGTTCTT CATTGCTTTT TTTGTTTCCC TGGTATTGAC ATGTGCTGTT 
TTCTTCATTC TTTACCGAAC CCAATTATTA AGATGGAGTT TCTATAGTGA AAACCAGGAA 
ATACAACATG AACTCAGTCA AAATCACAAA CTGGAGCATT CTCAGTTCAG TTCAGGTGAC 
CTCTTCAGTG AAGATGTTAT TATGAATGAC CAAATCATTG ATATACTGAC CTTTGAAGAG 
TCGGGGAATA TGCTTCAGGC TTTAGAAGAC TTGGAGGTTG CCAGTCTGAC TCGGGCAGAT 
GCTGATTTGG AGGCATATCG AATGCAGATC TGTAAAGAAG TGATTGCGAT AATGATGAAG 
AACATGGTTT TAAGTCACAG CCTCATTCCG CATGTGGAGA AGAGGATGAG TTCAATTTTC 
AAAAAGCAGT TTCTTACAAT GGAGAGAGAG TTTCAGGAAG AGTACGAAAG GAAGGTTGTG 
GCTTTAACAG CTGAATGTAA TCTGGAAACA AGGAAGAAGA TGGAGGCTCA ACGCCAGAAA 
GAGAGAGCTG CAAATGAAGA GGCTGAAGAG TTAATAAGGA AAGTGAATGA AATGCCTGCT 
GTAGAATACA AAAGTCTTTT GGATAGACTT CACAGTCTGG AGCAGGACCA TCTGAAGAGG 
TTCCTTCTGG TAAAGCAAGA GGAGTATTTT GCAAAGGCTT ACAGACAACT GGCTGTTTCC 
CGGAGAAAGG AGCTCCATAA CATCTTCTTT ACACAGATAA CAAATGCTAC TTTCAAGGGA 
GAACTGAAAC TGGAAATAGC AAAATCATTA GTGGAAGATT ACTCTAAAAT ACAGGGAGAC 
ATTGAAGAGC TAATGGATTT TTTGCAAGCC AGCAAGAAGT ATCACCTGAA CAGAAGATTT 
GCTTACAGAG AATATCTTAT AAAAAAGATA CAGTTAAGGG ATTCTCAGGC CTCTGCTCTT 
ATAAATGCAG CAGCAACTCA GATATTGAAT CTCATTAATA AGATGGAAAG GGCGGGTCAT 
CTACATGAAA GTCATTTGGG AATGCTGCTG GACCGAGCTG AGGCTGAAAT TAATTCTGTT 
AAACAAAAAT TCAATCATGA TTTAAAACAA GAAAAGCAAA AGCTTCAGCA GAAACTCATT 
ACCAAGCGAA AGTGGAAAAT GTTGCAAAAG AAAGAGCATT GGAAGGAGCA GCTGTCCATA 
GGGAACCCCT TCAAAACCTC AAGGGAGATC ACTGACTACC TGAGCCACTG GAAAACTGTT 
CTGAGTGATC ATGCCATTGA ATTTGAAGAA CTTACTGAAA AGCTGGATAA TGAAGGCAGT 
GAAGAGCTGA GAGACCTTAT ATTTAATCTA GCAGAGAAAA CTGTTGAAGA ACTTAAACTT 
GTTCAATATG GAGTATTTGT GCAAGAACTG ATAAAGCTCA GTGTGCCAAA GATGTTCCTG 
CTGGAAGTTG TAGAGGAGCA TAAAAAAGAA TTCATTGTTA AACATGAACA CCTTGAAAGG 
GAAGAACGTG AAAAGAGCAT GGCTGCTGAA GAGTTGCTTC AGCTCACCAG GCAAAAACTA 
AGCCAAGAGC TGGAAAGTAG TCTTTCACAA CAGAAAAAAT TAAGGAACTG GGAACAATCA 
GTATTCATGC AGCTTCTAAC TTTGCCCCTT TCACTGTCTG AAGAGGAATT GCTCAAAATG 
AGGCAAGAGT TCCACTGCTG CTTTTCACAG CTGGACAGCA GTTTGGCTCT GCCCAAGATA 
AGAGCAAGAG CTTTACTTCA GGCTTTTGAG CTGGAGTGGA GGGATGCGCA GCTGCTGAAA 
GTCAGTCAGA ATCTAGCAAT GACCAATAAG CAGCTGCATT CTAAAACGAA AAAAACAGGA 
TCCAAGAATC GAAATAAGAT TGATGTTTTG AAGAAATCTT TACAGGACAA AATTTTCATT 
TATGAAGACT CAGTGAAAGT TGAAAACTTG ACTAAGGTCA ATTATGAGTT GCTACTTGAG 
AAAGACTGTC AGATACATGA TCTGGAAAAT AAACTTGGAG AATATATTGC TTCTTTAGAG 
TTTCAAAAGA GTGTTAAAAA ATGCAAAATG TTGGAGCTCT ATGCAGCAGT CATAAGTGTG 
CAAGGTTTGC TCTTGGAAAA CCTGAGCATG TCAAAAACGC TGTCAAAATC GGAATGTATT 
CAGATTTTAG AAGCTCATAA TCCGGAGATT GAAGAACTTG ACAGGAAGCT GGAGTATGAA 
ATGCTGCATA AAGAGTCAGC ACAACAGCAG CAACATCTAA TGAATAAGCA GAGGTGGACA 
CCGGATGGAT TGAGACCTTC AAGTGAAGTC GTAGAAACAA ATGTAGACAG ACAGGTGACA 
TTTCTGCTAA GACAGGCCAT GAATAGATGT ATGCAGTTTA TAAATCTGCA CCGGCAAAGC 
TTGAGAGATG AGCAATGGAA TTGTATGGTG CTTGAAGATC TCTTGGAAAA TATAGAAATG 
GATGCATTTT TGGCACTTTA TAGTCAGGAG CTCCGACTGG CAGGTTATTT AGCCAGATTG 
ACAAGGGTAC CAATGGGGAT GTTGCACAGG ATTCTGAATT TGTTGCTGCC TTCCAGTTCG 
CAAAGTGAGG TTCTTTCTAT CCTTGATTCC ATTGGTGTAT ATTCAGATGG TGCTGCTGAG 
AGTGATAGTA ATGCAGATGA GTCTGGCAGC TGTAAAAAAA GGAGGAACCA AGAGTGTTGG 
CAAGCATTGG AGTACAAAGT AAGGGAAGAT CTGGTAACCA AAAGTTTGGA AAGGATCCTT 
TCTCTAAATA GGAGAAAAGA CAGCTTGATA AAGAAGAAGC AACTTGCCCT TCTGGAAAGA 
CCATCATTCG TTCACCTTGA AAGTTTGCCT ACACGTCCTC CTGTGGAACG GTCCAGTCCA 
CATGCTTCTT TTAACAGTGT GACAGCTGAA GCCATCGAGG TATTAGACAC AGGGGAAAAG 
ATATTTTTGT TTCGTGACCG GAGTGATCCA ACACTTTCTC TTCATAACTA TCCGAGAAAA 
AAGAAGAAGA ACTTTCTTAA TTCCAAAAAG GCTGCTCGTG CAAATATCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EVC2 ( XM_015285793.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015141279.1 Gallus gallus EvC ciliary complex subunit 2 (EVC2), transcript variant X1, mRNA.

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CloneID	OGa35733
Clone ID Related Accession (Same CDS sequence)	XM_015285793.1 , XM_015285793.2
Accession Version	XM_015285793.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	limbin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285793.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCGA AGGCTGAGGC CCGGTCTGGA TCGGGGCAGC GCGGCGCTCC GCGGGCCGGC 
CCGGTGCCGT GCGGGGCTGG GCGGCTCCTG GCCGGGCTGC TCGTGGTACT GCTGTCCTCC 
GCGCCCGCTC AGCCCCTCGG GGCGGCTGCC TGGTGCCCCA CGCAGGGACA CGGGGTGCCG 
GGCCCACCCC GGGGGCACTG CGGGAGGACG GCGGCTGGGG TTATTACAGA TGATCCGTGG 
CTTCAAAAAG AGATGAATTG TCTGCGGATG ACAGCACTGC AGTTGCCTGT GGTGGTTCAA 
GCAAGTGAAG TCCCTGATCT GATCTTGACT TCTGCAGCCT CGGAAGATCA GTGGAGCCAT 
TCAATATTTG CTCTGATACC CTCATGGACA AGGAAGATTT TGTTCAAACG AGAGTCTTCT 
GTTAACCACC AGCTGTCTTT TCCCCTTCCA CCACAGCCTG AAGATGTGTC CAACTTGTCA 
GCATCCTCTG GATTTGGAAT AACCCTTCAG AAATGTGCTG TGGTGGATGT TGAACATGAC 
CCTCAAACTG CATACATTAG ACTTCTGATT AATAACACCA ACTCACTGCT CTCAAGAAAC 
ATTACTGATC TTATCCTTCT GGACAATATC ACCGGTCTAC ATGTTCAAAA AGATAGTGGT 
AATGAGACAA GAGATGGGAT GCAAATTTAC AGGAAAAGAT TCTTGCAAGT TGGGGAATAT 
TTTGCAATCA ACTATACTGC ACTTCTTGAT GCAAAGAAAA CGTGGAAAGG TAGGGTCTTG 
ACACTGCCTG CACAGCTGAC TTTCAGGAGT TCATCACAGA ATCAAACAGA TCTTGTATCA 
TTGACCACAT TGTTCACAAT AACTGAAGAA GAAAAGACCG AGGTCTCCTA CAGCCATGCC 
ATCCATGCTT CAGGGTTCTT CATTGCTTTT TTTGTTTCCC TGGTATTGAC ATGTGCTGTT 
TTCTTCATTC TTTACCGAAC CCAATTATTA AGATGGAGTT TCTATAGTGA AAACCAGGAA 
ATACAACATG AACTCAGTCA AAATCACAAA CTGGAGCATT CTCAGTTCAG TTCAGGTGAC 
CTCTTCAGTG AAGATGTTAT TATGAATGAC CAAATCATTG ATATACTGAC CTTTGAAGAG 
TCGGGGAATA TGCTTCAGGC TTTAGAAGAC TTGGAGGTTG CCAGTCTGAC TCGGGCAGAT 
GCTGATTTGG AGGCATATCG AATGCAGATC TGTAAAGAAG TGATTGCGAT AATGATGAAG 
AACATGGTTT TAAGTCACAG CCTCATTCCG CATGTGGAGA AGAGGATGAG TTCAATTTTC 
AAAAAGCAGT TTCTTACAAT GGAGAGAGAG TTTCAGGAAG AGTACGAAAG GAAGGTTGTG 
GCTTTAACAG CTGAATGTAA TCTGGAAACA AGGAAGAAGA TGGAGGCTCA ACGCCAGAAA 
GAGAGAGCTG CAAATGAAGA GGCTGAAGAG TTAATAAGGA AAGTGAATGA AATGCCTGCT 
GTAGAATACA AAAGTCTTTT GGATAGACTT CACAGTCTGG AGCAGGACCA TCTGAAGAGG 
TTCCTTCTGG TAAAGCAAGA GGAGTATTTT GCAAAGGCTT ACAGACAACT GGCTGTTTCC 
CGGAGAAAGG AGCTCCATAA CATCTTCTTT ACACAGATAA CAAATGCTAC TTTCAAGGGA 
GAACTGAAAC TGGAAATAGC AAAATCATTA GTGGAAGATT ACTCTAAAAT ACAGGGAGAC 
ATTGAAGAGC TAATGGATTT TTTGCAAGCC AGCAAGAAGT ATCACCTGAA CAGAAGATTT 
GCTTACAGAG AATATCTTAT AAAAAAGATA CAGTTAAGGG ATTCTCAGGC CTCTGCTCTT 
ATAAATGCAG CAGCAACTCA GATATTGAAT CTCATTAATA AGATGGAAAG GGCGGGTCAT 
CTACATGAAA GTCATTTGGG AATGCTGCTG GACCGAGCTG AGGCTGAAAT TAATTCTGTT 
AAACAAAAAT TCAATCATGA TTTAAAACAA GAAAAGCAAA AGCTTCAGCA GAAACTCATT 
ACCAAGCGAA AGTGGAAAAT GTTGCAAAAG AAAGAGCATT GGAAGGAGCA GCTGTCCATA 
GGGAACCCCT TCAAAACCTC AAGGGAGATC ACTGACTACC TGAGCCACTG GAAAACTGTT 
CTGAGTGATC ATGCCATTGA ATTTGAAGAA CTTACTGAAA AGCTGGATAA TGAAGGCAGT 
GAAGAGCTGA GAGACCTTAT ATTTAATCTA GCAGAGAAAA CTGTTGAAGA ACTTAAACTT 
GTTCAATATG GAGTATTTGT GCAAGAACTG ATAAAGCTCA GTGTGCCAAA GATGTTCCTG 
CTGGAAGTTG TAGAGGAGCA TAAAAAAGAA TTCATTGTTA AACATGAACA CCTTGAAAGG 
GAAGAACGTG AAAAGAGCAT GGCTGCTGAA GAGTTGCTTC AGCTCACCAG GCAAAAACTA 
AGCCAAGAGC TGGAAAGTAG TCTTTCACAA CAGAAAAAAT TAAGGAACTG GGAACAATCA 
GTATTCATGC AGCTTCTAAC TTTGCCCCTT TCACTGTCTG AAGAGGAATT GCTCAAAATG 
AGGCAAGAGT TCCACTGCTG CTTTTCACAG CTGGACAGCA GTTTGGCTCT GCCCAAGATA 
AGAGCAAGAG CTTTACTTCA GGCTTTTGAG CTGGAGTGGA GGGATGCGCA GCTGCTGAAA 
GTCAGTCAGA ATCTAGCAAT GACCAATAAG CAGCTGCATT CTAAAACGAA AAAAACAGGA 
TCCAAGAATC GAAATAAGAT TGATGTTTTG AAGAAATCTT TACAGGACAA AATTTTCATT 
TATGAAGACT CAGTGAAAGT TGAAAACTTG ACTAAGGTCA ATTATGAGTT GCTACTTGAG 
AAAGACTGTC AGATACATGA TCTGGAAAAT AAACTTGGAG AATATATTGC TTCTTTAGAG 
TTTCAAAAGA GTGTTAAAAA ATGCAAAATG TTGGAGCTCT ATGCAGCAGT CATAAGTGTG 
CAAGGTTTGC TCTTGGAAAA CCTGAGCATG TCAAAAACGC TGTCAAAATC GGAATGTATT 
CAGATTTTAG AAGCTCATAA TCCGGAGATT GAAGAACTTG ACAGGAAGCT GGAGTATGAA 
ATGCTGCATA AAGAGTCAGC ACAACAGCAG CAACATCTAA TGAATAAGCA GAGGTGGACA 
CCGGATGGAT TGAGACCTTC AAGTGAAGTC GTAGAAACAA ATGTAGACAG ACAGGTGACA 
TTTCTGCTAA GACAGGCCAT GAATAGATGT ATGCAGTTTA TAAATCTGCA CCGGCAAAGC 
TTGAGAGATG AGCAATGGAA TTGTATGGTG CTTGAAGATC TCTTGGAAAA TATAGAAATG 
GATGCATTTT TGGCACTTTA TAGTCAGGAG CTCCGACTGG CAGGTTATTT AGCCAGATTG 
ACAAGGGTAC CAATGGGGAT GTTGCACAGG ATTCTGAATT TGTTGCTGCC TTCCAGTTCG 
CAAAGTGAGG TTCTTTCTAT CCTTGATTCC ATTGGTGTAT ATTCAGATGG TGCTGCTGAG 
AGTGATAGTA ATGCAGATGA GTCTGGCAGC TGTAAAAAAA GGAGGAACCA AGAGTGTTGG 
CAAGCATTGG AGTACAAAGT AAGGGAAGAT CTGGTAACCA AAAGTTTGGA AAGGATCCTT 
TCTCTAAATA GGAGAAAAGA CAGCTTGATA AAGAAGAAGC AACTTGCCCT TCTGGAAAGA 
CCATCATTCG TTCACCTTGA AAGTTTGCCT ACACGTCCTC CTGTGGAACG GTCCAGTCCA 
CATGCTTCTT TTAACAGTGT GACAGCTGAA GCCATCGAGG TATTAGACAC AGGGGAAAAG 
ATATTTTTGT TTCGTGACCG GAGTGATCCA ACACTTTCTC TTCATAACTA TCCGAGAAAA 
AAGAAGAAGA ACTTTCTTAA TTCCAAAAAG GCTGCTCGTG CAAATATCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015141279.1
CDS	16..3969
Translation	MDAKAEARSGSGQRGAPRAGPVPCGAGRLLAGLLVVLLSSAPAQPLGAAAWCPTQGHGVPGPPRGHCGRTAAGVITDDPWLQKEMNCLRMTALQLPVVVQASEVPDLILTSAASEDQWSHSIFALIPSWTRKILFKRESSVNHQLSFPLPPQPEDVSNLSASSGFGITLQKCAVVDVEHDPQTAYIRLLINNTNSLLSRNITDLILLDNITGLHVQKDSGNETRDGMQIYRKRFLQVGEYFAINYTALLDAKKTWKGRVLTLPAQLTFRSSSQNQTDLVSLTTLFTITEEEKTEVSYSHAIHASGFFIAFFVSLVLTCAVFFILYRTQLLRWSFYSENQEIQHELSQNHKLEHSQFSSGDLFSEDVIMNDQIIDILTFEESGNMLQALEDLEVASLTRADADLEAYRMQICKEVIAIMMKNMVLSHSLIPHVEKRMSSIFKKQFLTMEREFQEEYERKVVALTAECNLETRKKMEAQRQKERAANEEAEELIRKVNEMPAVEYKSLLDRLHSLEQDHLKRFLLVKQEEYFAKAYRQLAVSRRKELHNIFFTQITNATFKGELKLEIAKSLVEDYSKIQGDIEELMDFLQASKKYHLNRRFAYREYLIKKIQLRDSQASALINAAATQILNLINKMERAGHLHESHLGMLLDRAEAEINSVKQKFNHDLKQEKQKLQQKLITKRKWKMLQKKEHWKEQLSIGNPFKTSREITDYLSHWKTVLSDHAIEFEELTEKLDNEGSEELRDLIFNLAEKTVEELKLVQYGVFVQELIKLSVPKMFLLEVVEEHKKEFIVKHEHLEREEREKSMAAEELLQLTRQKLSQELESSLSQQKKLRNWEQSVFMQLLTLPLSLSEEELLKMRQEFHCCFSQLDSSLALPKIRARALLQAFELEWRDAQLLKVSQNLAMTNKQLHSKTKKTGSKNRNKIDVLKKSLQDKIFIYEDSVKVENLTKVNYELLLEKDCQIHDLENKLGEYIASLEFQKSVKKCKMLELYAAVISVQGLLLENLSMSKTLSKSECIQILEAHNPEIEELDRKLEYEMLHKESAQQQQHLMNKQRWTPDGLRPSSEVVETNVDRQVTFLLRQAMNRCMQFINLHRQSLRDEQWNCMVLEDLLENIEMDAFLALYSQELRLAGYLARLTRVPMGMLHRILNLLLPSSSQSEVLSILDSIGVYSDGAAESDSNADESGSCKKRRNQECWQALEYKVREDLVTKSLERILSLNRRKDSLIKKKQLALLERPSFVHLESLPTRPPVERSSPHASFNSVTAEAIEVLDTGEKIFLFRDRSDPTLSLHNYPRKKKKNFLNSKKAARANIN

Target ORF information:

RefSeq Version	XM_015285793.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus EvC ciliary complex subunit 2 (EVC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285793.2

ORF Insert Sequence:

ATGGATGCGA AGGCTGAGGC CCGGTCTGGA TCGGGGCAGC GCGGCGCTCC GCGGGCCGGC 
CCGGTGCCGT GCGGGGCTGG GCGGCTCCTG GCCGGGCTGC TCGTGGTACT GCTGTCCTCC 
GCGCCCGCTC AGCCCCTCGG GGCGGCTGCC TGGTGCCCCA CGCAGGGACA CGGGGTGCCG 
GGCCCACCCC GGGGGCACTG CGGGAGGACG GCGGCTGGGG TTATTACAGA TGATCCGTGG 
CTTCAAAAAG AGATGAATTG TCTGCGGATG ACAGCACTGC AGTTGCCTGT GGTGGTTCAA 
GCAAGTGAAG TCCCTGATCT GATCTTGACT TCTGCAGCCT CGGAAGATCA GTGGAGCCAT 
TCAATATTTG CTCTGATACC CTCATGGACA AGGAAGATTT TGTTCAAACG AGAGTCTTCT 
GTTAACCACC AGCTGTCTTT TCCCCTTCCA CCACAGCCTG AAGATGTGTC CAACTTGTCA 
GCATCCTCTG GATTTGGAAT AACCCTTCAG AAATGTGCTG TGGTGGATGT TGAACATGAC 
CCTCAAACTG CATACATTAG ACTTCTGATT AATAACACCA ACTCACTGCT CTCAAGAAAC 
ATTACTGATC TTATCCTTCT GGACAATATC ACCGGTCTAC ATGTTCAAAA AGATAGTGGT 
AATGAGACAA GAGATGGGAT GCAAATTTAC AGGAAAAGAT TCTTGCAAGT TGGGGAATAT 
TTTGCAATCA ACTATACTGC ACTTCTTGAT GCAAAGAAAA CGTGGAAAGG TAGGGTCTTG 
ACACTGCCTG CACAGCTGAC TTTCAGGAGT TCATCACAGA ATCAAACAGA TCTTGTATCA 
TTGACCACAT TGTTCACAAT AACTGAAGAA GAAAAGACCG AGGTCTCCTA CAGCCATGCC 
ATCCATGCTT CAGGGTTCTT CATTGCTTTT TTTGTTTCCC TGGTATTGAC ATGTGCTGTT 
TTCTTCATTC TTTACCGAAC CCAATTATTA AGATGGAGTT TCTATAGTGA AAACCAGGAA 
ATACAACATG AACTCAGTCA AAATCACAAA CTGGAGCATT CTCAGTTCAG TTCAGGTGAC 
CTCTTCAGTG AAGATGTTAT TATGAATGAC CAAATCATTG ATATACTGAC CTTTGAAGAG 
TCGGGGAATA TGCTTCAGGC TTTAGAAGAC TTGGAGGTTG CCAGTCTGAC TCGGGCAGAT 
GCTGATTTGG AGGCATATCG AATGCAGATC TGTAAAGAAG TGATTGCGAT AATGATGAAG 
AACATGGTTT TAAGTCACAG CCTCATTCCG CATGTGGAGA AGAGGATGAG TTCAATTTTC 
AAAAAGCAGT TTCTTACAAT GGAGAGAGAG TTTCAGGAAG AGTACGAAAG GAAGGTTGTG 
GCTTTAACAG CTGAATGTAA TCTGGAAACA AGGAAGAAGA TGGAGGCTCA ACGCCAGAAA 
GAGAGAGCTG CAAATGAAGA GGCTGAAGAG TTAATAAGGA AAGTGAATGA AATGCCTGCT 
GTAGAATACA AAAGTCTTTT GGATAGACTT CACAGTCTGG AGCAGGACCA TCTGAAGAGG 
TTCCTTCTGG TAAAGCAAGA GGAGTATTTT GCAAAGGCTT ACAGACAACT GGCTGTTTCC 
CGGAGAAAGG AGCTCCATAA CATCTTCTTT ACACAGATAA CAAATGCTAC TTTCAAGGGA 
GAACTGAAAC TGGAAATAGC AAAATCATTA GTGGAAGATT ACTCTAAAAT ACAGGGAGAC 
ATTGAAGAGC TAATGGATTT TTTGCAAGCC AGCAAGAAGT ATCACCTGAA CAGAAGATTT 
GCTTACAGAG AATATCTTAT AAAAAAGATA CAGTTAAGGG ATTCTCAGGC CTCTGCTCTT 
ATAAATGCAG CAGCAACTCA GATATTGAAT CTCATTAATA AGATGGAAAG GGCGGGTCAT 
CTACATGAAA GTCATTTGGG AATGCTGCTG GACCGAGCTG AGGCTGAAAT TAATTCTGTT 
AAACAAAAAT TCAATCATGA TTTAAAACAA GAAAAGCAAA AGCTTCAGCA GAAACTCATT 
ACCAAGCGAA AGTGGAAAAT GTTGCAAAAG AAAGAGCATT GGAAGGAGCA GCTGTCCATA 
GGGAACCCCT TCAAAACCTC AAGGGAGATC ACTGACTACC TGAGCCACTG GAAAACTGTT 
CTGAGTGATC ATGCCATTGA ATTTGAAGAA CTTACTGAAA AGCTGGATAA TGAAGGCAGT 
GAAGAGCTGA GAGACCTTAT ATTTAATCTA GCAGAGAAAA CTGTTGAAGA ACTTAAACTT 
GTTCAATATG GAGTATTTGT GCAAGAACTG ATAAAGCTCA GTGTGCCAAA GATGTTCCTG 
CTGGAAGTTG TAGAGGAGCA TAAAAAAGAA TTCATTGTTA AACATGAACA CCTTGAAAGG 
GAAGAACGTG AAAAGAGCAT GGCTGCTGAA GAGTTGCTTC AGCTCACCAG GCAAAAACTA 
AGCCAAGAGC TGGAAAGTAG TCTTTCACAA CAGAAAAAAT TAAGGAACTG GGAACAATCA 
GTATTCATGC AGCTTCTAAC TTTGCCCCTT TCACTGTCTG AAGAGGAATT GCTCAAAATG 
AGGCAAGAGT TCCACTGCTG CTTTTCACAG CTGGACAGCA GTTTGGCTCT GCCCAAGATA 
AGAGCAAGAG CTTTACTTCA GGCTTTTGAG CTGGAGTGGA GGGATGCGCA GCTGCTGAAA 
GTCAGTCAGA ATCTAGCAAT GACCAATAAG CAGCTGCATT CTAAAACGAA AAAAACAGGA 
TCCAAGAATC GAAATAAGAT TGATGTTTTG AAGAAATCTT TACAGGACAA AATTTTCATT 
TATGAAGACT CAGTGAAAGT TGAAAACTTG ACTAAGGTCA ATTATGAGTT GCTACTTGAG 
AAAGACTGTC AGATACATGA TCTGGAAAAT AAACTTGGAG AATATATTGC TTCTTTAGAG 
TTTCAAAAGA GTGTTAAAAA ATGCAAAATG TTGGAGCTCT ATGCAGCAGT CATAAGTGTG 
CAAGGTTTGC TCTTGGAAAA CCTGAGCATG TCAAAAACGC TGTCAAAATC GGAATGTATT 
CAGATTTTAG AAGCTCATAA TCCGGAGATT GAAGAACTTG ACAGGAAGCT GGAGTATGAA 
ATGCTGCATA AAGAGTCAGC ACAACAGCAG CAACATCTAA TGAATAAGCA GAGGTGGACA 
CCGGATGGAT TGAGACCTTC AAGTGAAGTC GTAGAAACAA ATGTAGACAG ACAGGTGACA 
TTTCTGCTAA GACAGGCCAT GAATAGATGT ATGCAGTTTA TAAATCTGCA CCGGCAAAGC 
TTGAGAGATG AGCAATGGAA TTGTATGGTG CTTGAAGATC TCTTGGAAAA TATAGAAATG 
GATGCATTTT TGGCACTTTA TAGTCAGGAG CTCCGACTGG CAGGTTATTT AGCCAGATTG 
ACAAGGGTAC CAATGGGGAT GTTGCACAGG ATTCTGAATT TGTTGCTGCC TTCCAGTTCG 
CAAAGTGAGG TTCTTTCTAT CCTTGATTCC ATTGGTGTAT ATTCAGATGG TGCTGCTGAG 
AGTGATAGTA ATGCAGATGA GTCTGGCAGC TGTAAAAAAA GGAGGAACCA AGAGTGTTGG 
CAAGCATTGG AGTACAAAGT AAGGGAAGAT CTGGTAACCA AAAGTTTGGA AAGGATCCTT 
TCTCTAAATA GGAGAAAAGA CAGCTTGATA AAGAAGAAGC AACTTGCCCT TCTGGAAAGA 
CCATCATTCG TTCACCTTGA AAGTTTGCCT ACACGTCCTC CTGTGGAACG GTCCAGTCCA 
CATGCTTCTT TTAACAGTGT GACAGCTGAA GCCATCGAGG TATTAGACAC AGGGGAAAAG 
ATATTTTTGT TTCGTGACCG GAGTGATCCA ACACTTTCTC TTCATAACTA TCCGAGAAAA 
AAGAAGAAGA ACTTTCTTAA TTCCAAAAAG GCTGCTCGTG CAAATATCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EVC2 ( XM_015285796.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015141282.1 Gallus gallus Ellis van Creveld syndrome 2 (EVC2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OGa35736
Clone ID Related Accession (Same CDS sequence)	XM_015285796.1 , XM_015285796.2
Accession Version	XM_015285796.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	limbin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455863.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCGA AGGCTGAGGC CCGGTCTGGA TCGGGGCAGC GCGGCGCTCC GCGGGCCGGC 
CCGGTGCCGT GCGGGGCTGG GCGGCTCCTG GCCGGGCTGC TCGTGGTACT GCTGTCCTCC 
GCGCCCGCTC AGCCCCTCGG GGCGGCTGCC TGGTGCCCCA CGCAGGGACA CGGGGTGCCG 
GGCCCACCCC GGGGGCACTG CGGGAGGACG GCGGCTGGGG TTATTACAGA TGATCCGTGG 
CTTCAAAAAG AGATGAATTG TCTGCGGATG ACAGCACTGC AGTTGCCTGT GGTGGTTCAA 
GCAAGTGAAG TCCCTGATCT GATCTTGACT TCTGCAGCCT CGGAAGATCA GTGGAGCCAT 
TCAATATTTG CTCTGATACC CTCATGGACA AGGAAGATTT TGTTCAAACG AGAGTCTTCT 
GTTAACCACC AGCTGTCTTT TCCCCTTCCA CCACAGCCTG AAGATGTGTC CAACTTGTCA 
GCATCCTCTG GATTTGGAAT AACCCTTCAG AAATGTGCTG TGGTGGATGT TGAACATGAC 
CCTCAAACTG CATACATTAG ACTTCTGATT AATAACACCA ACTCACTGCT CTCAAGAAAC 
ATTACTGATC TTATCCTTCT GGACAATATC ACCGGTCTAC ATGTTCAAAA AGATAGTGGT 
AATGAGACAA GAGATGGGAT GCAAATTTAC AGGAAAAGAT TCTTGCAAGT TGGGGAATAT 
TTTGCAATCA ACTATACTGC ACTTCTTGAT GCAAAGAAAA CGTGGAAAGG TAGGGTCTTG 
ACACTGCCTG CACAGCTGAC TTTCAGGAGT TCATCACAGA ATCAAACAGA TCTTGTATCA 
TTGACCACAT TGTTCACAAT AACTGAAGAA GAAAAGACCG AGGTCTCCTA CAGCCATGCC 
ATCCATGCTT CAGGGTTCTT CATTGCTTTT TTTGTTTCCC TGGTATTGAC ATGTGCTGTT 
TTCTTCATTC TTTACCGAAC CCAATTATTA AGATGGAGTT TCTATAGTGA AAACCAGGAA 
ATACAACATG AACTCAGTCA AAATCACAAA CTGGAGCATT CTCAGTTCAG TTCAGGTGAC 
CTCTTCAGTG AAGATGTTAT TATGAATGAC CAAATCATTG ATATACTGAC CTTTGAAGAG 
TCGGGGAATA TGCTTCAGGC TTTAGAAGAG GCGGGTCATC TACATGAAAG TCATTTGGGA 
ATGCTGCTGG ACCGAGCTGA GGCTGAAATT AATTCTGTTA AACAAAAATT CAATCATGAT 
TTAAAACAAG AAAAGCAAAA GCTTCAGCAG AAACTCATTA CCAAGCGAAA GTGGAAAATG 
TTGCAAAAGA AAGAGCATTG GAAGGAGCAG CTGTCCATAG GGAACCCCTT CAAAACCTCA 
AGGGAGATCA CTGACTACCT GAGCCACTGG AAAACTGTTC TGAGTGATCA TGCCATTGAA 
TTTGAAGAAC TTACTGAAAA GCTGGATAAT GAAGGCAGTG AAGAGCTGAG AGACCTTATA 
TTTAATCTAG CAGAGAAAAC TGTTGAAGAA CTTAAACTTG TTCAATATGG AGTATTTGTG 
CAAGAACTGA TAAAGCTCAG TGTGCCAAAG ATGTTCCTGC TGGAAGTTGT AGAGGAGCAT 
AAAAAAGAAT TCATTGTTAA ACATGAACAC CTTGAAAGGG AAGAACGTGA AAAGAGCATG 
GCTGCTGAAG AGTTGCTTCA GCTCACCAGG CAAAAACTAA GCCAAGAGCT GGAAAGTAGT 
CTTTCACAAC AGAAAAAATT AAGGAACTGG GAACAATCAG TATTCATGCA GCTTCTAACT 
TTGCCCCTTT CACTGTCTGA AGAGGAATTG CTCAAAATGA GGCAAGAGTT CCACTGCTGC 
TTTTCACAGC TGGACAGCAG TTTGGCTCTG CCCAAGATAA GAGCAAGAGC TTTACTTCAG 
GCTTTTGAGC TGGAGTGGAG GGATGCGCAG CTGCTGAAAG TCAGTCAGAA TCTAGCAATG 
ACCAATAAGC AGCTGCATTC TAAAACGAAA AAAACAGGAT CCAAGAATCG AAATAAGATT 
GATGTTTTGA AGAAATCTTT ACAGGACAAA ATTTTCATTT ATGAAGACTC AGTGAAAGTT 
GAAAACTTGA CTAAGGTCAA TTATGAGTTG CTACTTGAGA AAGACTGTCA GATACATGAT 
CTGGAAAATA AACTTGGAGA ATATATTGCT TCTTTAGAGT TTCAAAAGAG TGTTAAAAAA 
TGCAAAATGT TGGAGCTCTA TGCAGCAGTC ATAAGTGTGC AAGGTTTGCT CTTGGAAAAC 
CTGAGCATGT CAAAAACGCT GTCAAAATCG GAATGTATTC AGATTTTAGA AGCTCATAAT 
CCGGAGATTG AAGAACTTGA CAGGAAGCTG GAGTATGAAA TGCTGCATAA AGAGTCAGCA 
CAACAGCAGC AACATCTAAT GAATAAGCAG AGGTGGACAC CGGATGGATT GAGACCTTCA 
AGTGAAGTCG TAGAAACAAA TGTAGACAGA CAGGTGACAT TTCTGCTAAG ACAGGCCATG 
AATAGATGTA TGCAGTTTAT AAATCTGCAC CGGCAAAGCT TGAGAGATGA GCAATGGAAT 
TGTATGGTGC TTGAAGATCT CTTGGAAAAT ATAGAAATGG ATGCATTTTT GGCACTTTAT 
AGTCAGGAGC TCCGACTGGC AGGTTATTTA GCCAGATTGA CAAGGGTACC AATGGGGATG 
TTGCACAGGA TTCTGAATTT GTTGCTGCCT TCCAGTTCGC AAAGTGAGGT TCTTTCTATC 
CTTGATTCCA TTGGTGTATA TTCAGATGGT GCTGCTGAGA GTGATAGTAA TGCAGATGAG 
TCTGGCAGCT GTAAAAAAAG GAGGAACCAA GAGTGTTGGC AAGCATTGGA GTACAAAGTA 
AGGGAAGATC TGGTAACCAA AAGTTTGGAA AGGATCCTTT CTCTAAATAG GAGAAAAGAC 
AGCTTGATAA AGAAGAAGCA ACTTGCCCTT CTGGAAAGAC CATCATTCGT TCACCTTGAA 
AGTTTGCCTA CACGTCCTCC TGTGGAACGG TCCAGTCCAC ATGCTTCTTT TAACAGTGTG 
ACAGCTGAAG CCATCGAGGT ATTAGACACA GGGGAAAAGA TATTTTTGTT TCGTGACCGG 
AGTGATCCAA CACTTTCTCT TCATAACTAT CCGAGAAAAA AGAAGAAGAA CTTTCTTAAT 
TCCAAAAAGG CTGCTCGTGC AAATATCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015141282.1
CDS	24..3236
Translation	MDAKAEARSGSGQRGAPRAGPVPCGAGRLLAGLLVVLLSSAPAQPLGAAAWCPTQGHGVPGPPRGHCGRTAAGVITDDPWLQKEMNCLRMTALQLPVVVQASEVPDLILTSAASEDQWSHSIFALIPSWTRKILFKRESSVNHQLSFPLPPQPEDVSNLSASSGFGITLQKCAVVDVEHDPQTAYIRLLINNTNSLLSRNITDLILLDNITGLHVQKDSGNETRDGMQIYRKRFLQVGEYFAINYTALLDAKKTWKGRVLTLPAQLTFRSSSQNQTDLVSLTTLFTITEEEKTEVSYSHAIHASGFFIAFFVSLVLTCAVFFILYRTQLLRWSFYSENQEIQHELSQNHKLEHSQFSSGDLFSEDVIMNDQIIDILTFEESGNMLQALEEAGHLHESHLGMLLDRAEAEINSVKQKFNHDLKQEKQKLQQKLITKRKWKMLQKKEHWKEQLSIGNPFKTSREITDYLSHWKTVLSDHAIEFEELTEKLDNEGSEELRDLIFNLAEKTVEELKLVQYGVFVQELIKLSVPKMFLLEVVEEHKKEFIVKHEHLEREEREKSMAAEELLQLTRQKLSQELESSLSQQKKLRNWEQSVFMQLLTLPLSLSEEELLKMRQEFHCCFSQLDSSLALPKIRARALLQAFELEWRDAQLLKVSQNLAMTNKQLHSKTKKTGSKNRNKIDVLKKSLQDKIFIYEDSVKVENLTKVNYELLLEKDCQIHDLENKLGEYIASLEFQKSVKKCKMLELYAAVISVQGLLLENLSMSKTLSKSECIQILEAHNPEIEELDRKLEYEMLHKESAQQQQHLMNKQRWTPDGLRPSSEVVETNVDRQVTFLLRQAMNRCMQFINLHRQSLRDEQWNCMVLEDLLENIEMDAFLALYSQELRLAGYLARLTRVPMGMLHRILNLLLPSSSQSEVLSILDSIGVYSDGAAESDSNADESGSCKKRRNQECWQALEYKVREDLVTKSLERILSLNRRKDSLIKKKQLALLERPSFVHLESLPTRPPVERSSPHASFNSVTAEAIEVLDTGEKIFLFRDRSDPTLSLHNYPRKKKKNFLNSKKAARANIN

Target ORF information:

RefSeq Version	XM_015285796.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Ellis van Creveld syndrome 2 (EVC2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285796.1

ORF Insert Sequence:

ATGGATGCGA AGGCTGAGGC CCGGTCTGGA TCGGGGCAGC GCGGCGCTCC GCGGGCCGGC 
CCGGTGCCGT GCGGGGCTGG GCGGCTCCTG GCCGGGCTGC TCGTGGTACT GCTGTCCTCC 
GCGCCCGCTC AGCCCCTCGG GGCGGCTGCC TGGTGCCCCA CGCAGGGACA CGGGGTGCCG 
GGCCCACCCC GGGGGCACTG CGGGAGGACG GCGGCTGGGG TTATTACAGA TGATCCGTGG 
CTTCAAAAAG AGATGAATTG TCTGCGGATG ACAGCACTGC AGTTGCCTGT GGTGGTTCAA 
GCAAGTGAAG TCCCTGATCT GATCTTGACT TCTGCAGCCT CGGAAGATCA GTGGAGCCAT 
TCAATATTTG CTCTGATACC CTCATGGACA AGGAAGATTT TGTTCAAACG AGAGTCTTCT 
GTTAACCACC AGCTGTCTTT TCCCCTTCCA CCACAGCCTG AAGATGTGTC CAACTTGTCA 
GCATCCTCTG GATTTGGAAT AACCCTTCAG AAATGTGCTG TGGTGGATGT TGAACATGAC 
CCTCAAACTG CATACATTAG ACTTCTGATT AATAACACCA ACTCACTGCT CTCAAGAAAC 
ATTACTGATC TTATCCTTCT GGACAATATC ACCGGTCTAC ATGTTCAAAA AGATAGTGGT 
AATGAGACAA GAGATGGGAT GCAAATTTAC AGGAAAAGAT TCTTGCAAGT TGGGGAATAT 
TTTGCAATCA ACTATACTGC ACTTCTTGAT GCAAAGAAAA CGTGGAAAGG TAGGGTCTTG 
ACACTGCCTG CACAGCTGAC TTTCAGGAGT TCATCACAGA ATCAAACAGA TCTTGTATCA 
TTGACCACAT TGTTCACAAT AACTGAAGAA GAAAAGACCG AGGTCTCCTA CAGCCATGCC 
ATCCATGCTT CAGGGTTCTT CATTGCTTTT TTTGTTTCCC TGGTATTGAC ATGTGCTGTT 
TTCTTCATTC TTTACCGAAC CCAATTATTA AGATGGAGTT TCTATAGTGA AAACCAGGAA 
ATACAACATG AACTCAGTCA AAATCACAAA CTGGAGCATT CTCAGTTCAG TTCAGGTGAC 
CTCTTCAGTG AAGATGTTAT TATGAATGAC CAAATCATTG ATATACTGAC CTTTGAAGAG 
TCGGGGAATA TGCTTCAGGC TTTAGAAGAG GCGGGTCATC TACATGAAAG TCATTTGGGA 
ATGCTGCTGG ACCGAGCTGA GGCTGAAATT AATTCTGTTA AACAAAAATT CAATCATGAT 
TTAAAACAAG AAAAGCAAAA GCTTCAGCAG AAACTCATTA CCAAGCGAAA GTGGAAAATG 
TTGCAAAAGA AAGAGCATTG GAAGGAGCAG CTGTCCATAG GGAACCCCTT CAAAACCTCA 
AGGGAGATCA CTGACTACCT GAGCCACTGG AAAACTGTTC TGAGTGATCA TGCCATTGAA 
TTTGAAGAAC TTACTGAAAA GCTGGATAAT GAAGGCAGTG AAGAGCTGAG AGACCTTATA 
TTTAATCTAG CAGAGAAAAC TGTTGAAGAA CTTAAACTTG TTCAATATGG AGTATTTGTG 
CAAGAACTGA TAAAGCTCAG TGTGCCAAAG ATGTTCCTGC TGGAAGTTGT AGAGGAGCAT 
AAAAAAGAAT TCATTGTTAA ACATGAACAC CTTGAAAGGG AAGAACGTGA AAAGAGCATG 
GCTGCTGAAG AGTTGCTTCA GCTCACCAGG CAAAAACTAA GCCAAGAGCT GGAAAGTAGT 
CTTTCACAAC AGAAAAAATT AAGGAACTGG GAACAATCAG TATTCATGCA GCTTCTAACT 
TTGCCCCTTT CACTGTCTGA AGAGGAATTG CTCAAAATGA GGCAAGAGTT CCACTGCTGC 
TTTTCACAGC TGGACAGCAG TTTGGCTCTG CCCAAGATAA GAGCAAGAGC TTTACTTCAG 
GCTTTTGAGC TGGAGTGGAG GGATGCGCAG CTGCTGAAAG TCAGTCAGAA TCTAGCAATG 
ACCAATAAGC AGCTGCATTC TAAAACGAAA AAAACAGGAT CCAAGAATCG AAATAAGATT 
GATGTTTTGA AGAAATCTTT ACAGGACAAA ATTTTCATTT ATGAAGACTC AGTGAAAGTT 
GAAAACTTGA CTAAGGTCAA TTATGAGTTG CTACTTGAGA AAGACTGTCA GATACATGAT 
CTGGAAAATA AACTTGGAGA ATATATTGCT TCTTTAGAGT TTCAAAAGAG TGTTAAAAAA 
TGCAAAATGT TGGAGCTCTA TGCAGCAGTC ATAAGTGTGC AAGGTTTGCT CTTGGAAAAC 
CTGAGCATGT CAAAAACGCT GTCAAAATCG GAATGTATTC AGATTTTAGA AGCTCATAAT 
CCGGAGATTG AAGAACTTGA CAGGAAGCTG GAGTATGAAA TGCTGCATAA AGAGTCAGCA 
CAACAGCAGC AACATCTAAT GAATAAGCAG AGGTGGACAC CGGATGGATT GAGACCTTCA 
AGTGAAGTCG TAGAAACAAA TGTAGACAGA CAGGTGACAT TTCTGCTAAG ACAGGCCATG 
AATAGATGTA TGCAGTTTAT AAATCTGCAC CGGCAAAGCT TGAGAGATGA GCAATGGAAT 
TGTATGGTGC TTGAAGATCT CTTGGAAAAT ATAGAAATGG ATGCATTTTT GGCACTTTAT 
AGTCAGGAGC TCCGACTGGC AGGTTATTTA GCCAGATTGA CAAGGGTACC AATGGGGATG 
TTGCACAGGA TTCTGAATTT GTTGCTGCCT TCCAGTTCGC AAAGTGAGGT TCTTTCTATC 
CTTGATTCCA TTGGTGTATA TTCAGATGGT GCTGCTGAGA GTGATAGTAA TGCAGATGAG 
TCTGGCAGCT GTAAAAAAAG GAGGAACCAA GAGTGTTGGC AAGCATTGGA GTACAAAGTA 
AGGGAAGATC TGGTAACCAA AAGTTTGGAA AGGATCCTTT CTCTAAATAG GAGAAAAGAC 
AGCTTGATAA AGAAGAAGCA ACTTGCCCTT CTGGAAAGAC CATCATTCGT TCACCTTGAA 
AGTTTGCCTA CACGTCCTCC TGTGGAACGG TCCAGTCCAC ATGCTTCTTT TAACAGTGTG 
ACAGCTGAAG CCATCGAGGT ATTAGACACA GGGGAAAAGA TATTTTTGTT TCGTGACCGG 
AGTGATCCAA CACTTTCTCT TCATAACTAT CCGAGAAAAA AGAAGAAGAA CTTTCTTAAT 
TCCAAAAAGG CTGCTCGTGC AAATATCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EVC2 ( XM_015285796.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015141282.1 Gallus gallus EvC ciliary complex subunit 2 (EVC2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OGa35736
Clone ID Related Accession (Same CDS sequence)	XM_015285796.1 , XM_015285796.2
Accession Version	XM_015285796.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	limbin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285796.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCGA AGGCTGAGGC CCGGTCTGGA TCGGGGCAGC GCGGCGCTCC GCGGGCCGGC 
CCGGTGCCGT GCGGGGCTGG GCGGCTCCTG GCCGGGCTGC TCGTGGTACT GCTGTCCTCC 
GCGCCCGCTC AGCCCCTCGG GGCGGCTGCC TGGTGCCCCA CGCAGGGACA CGGGGTGCCG 
GGCCCACCCC GGGGGCACTG CGGGAGGACG GCGGCTGGGG TTATTACAGA TGATCCGTGG 
CTTCAAAAAG AGATGAATTG TCTGCGGATG ACAGCACTGC AGTTGCCTGT GGTGGTTCAA 
GCAAGTGAAG TCCCTGATCT GATCTTGACT TCTGCAGCCT CGGAAGATCA GTGGAGCCAT 
TCAATATTTG CTCTGATACC CTCATGGACA AGGAAGATTT TGTTCAAACG AGAGTCTTCT 
GTTAACCACC AGCTGTCTTT TCCCCTTCCA CCACAGCCTG AAGATGTGTC CAACTTGTCA 
GCATCCTCTG GATTTGGAAT AACCCTTCAG AAATGTGCTG TGGTGGATGT TGAACATGAC 
CCTCAAACTG CATACATTAG ACTTCTGATT AATAACACCA ACTCACTGCT CTCAAGAAAC 
ATTACTGATC TTATCCTTCT GGACAATATC ACCGGTCTAC ATGTTCAAAA AGATAGTGGT 
AATGAGACAA GAGATGGGAT GCAAATTTAC AGGAAAAGAT TCTTGCAAGT TGGGGAATAT 
TTTGCAATCA ACTATACTGC ACTTCTTGAT GCAAAGAAAA CGTGGAAAGG TAGGGTCTTG 
ACACTGCCTG CACAGCTGAC TTTCAGGAGT TCATCACAGA ATCAAACAGA TCTTGTATCA 
TTGACCACAT TGTTCACAAT AACTGAAGAA GAAAAGACCG AGGTCTCCTA CAGCCATGCC 
ATCCATGCTT CAGGGTTCTT CATTGCTTTT TTTGTTTCCC TGGTATTGAC ATGTGCTGTT 
TTCTTCATTC TTTACCGAAC CCAATTATTA AGATGGAGTT TCTATAGTGA AAACCAGGAA 
ATACAACATG AACTCAGTCA AAATCACAAA CTGGAGCATT CTCAGTTCAG TTCAGGTGAC 
CTCTTCAGTG AAGATGTTAT TATGAATGAC CAAATCATTG ATATACTGAC CTTTGAAGAG 
TCGGGGAATA TGCTTCAGGC TTTAGAAGAG GCGGGTCATC TACATGAAAG TCATTTGGGA 
ATGCTGCTGG ACCGAGCTGA GGCTGAAATT AATTCTGTTA AACAAAAATT CAATCATGAT 
TTAAAACAAG AAAAGCAAAA GCTTCAGCAG AAACTCATTA CCAAGCGAAA GTGGAAAATG 
TTGCAAAAGA AAGAGCATTG GAAGGAGCAG CTGTCCATAG GGAACCCCTT CAAAACCTCA 
AGGGAGATCA CTGACTACCT GAGCCACTGG AAAACTGTTC TGAGTGATCA TGCCATTGAA 
TTTGAAGAAC TTACTGAAAA GCTGGATAAT GAAGGCAGTG AAGAGCTGAG AGACCTTATA 
TTTAATCTAG CAGAGAAAAC TGTTGAAGAA CTTAAACTTG TTCAATATGG AGTATTTGTG 
CAAGAACTGA TAAAGCTCAG TGTGCCAAAG ATGTTCCTGC TGGAAGTTGT AGAGGAGCAT 
AAAAAAGAAT TCATTGTTAA ACATGAACAC CTTGAAAGGG AAGAACGTGA AAAGAGCATG 
GCTGCTGAAG AGTTGCTTCA GCTCACCAGG CAAAAACTAA GCCAAGAGCT GGAAAGTAGT 
CTTTCACAAC AGAAAAAATT AAGGAACTGG GAACAATCAG TATTCATGCA GCTTCTAACT 
TTGCCCCTTT CACTGTCTGA AGAGGAATTG CTCAAAATGA GGCAAGAGTT CCACTGCTGC 
TTTTCACAGC TGGACAGCAG TTTGGCTCTG CCCAAGATAA GAGCAAGAGC TTTACTTCAG 
GCTTTTGAGC TGGAGTGGAG GGATGCGCAG CTGCTGAAAG TCAGTCAGAA TCTAGCAATG 
ACCAATAAGC AGCTGCATTC TAAAACGAAA AAAACAGGAT CCAAGAATCG AAATAAGATT 
GATGTTTTGA AGAAATCTTT ACAGGACAAA ATTTTCATTT ATGAAGACTC AGTGAAAGTT 
GAAAACTTGA CTAAGGTCAA TTATGAGTTG CTACTTGAGA AAGACTGTCA GATACATGAT 
CTGGAAAATA AACTTGGAGA ATATATTGCT TCTTTAGAGT TTCAAAAGAG TGTTAAAAAA 
TGCAAAATGT TGGAGCTCTA TGCAGCAGTC ATAAGTGTGC AAGGTTTGCT CTTGGAAAAC 
CTGAGCATGT CAAAAACGCT GTCAAAATCG GAATGTATTC AGATTTTAGA AGCTCATAAT 
CCGGAGATTG AAGAACTTGA CAGGAAGCTG GAGTATGAAA TGCTGCATAA AGAGTCAGCA 
CAACAGCAGC AACATCTAAT GAATAAGCAG AGGTGGACAC CGGATGGATT GAGACCTTCA 
AGTGAAGTCG TAGAAACAAA TGTAGACAGA CAGGTGACAT TTCTGCTAAG ACAGGCCATG 
AATAGATGTA TGCAGTTTAT AAATCTGCAC CGGCAAAGCT TGAGAGATGA GCAATGGAAT 
TGTATGGTGC TTGAAGATCT CTTGGAAAAT ATAGAAATGG ATGCATTTTT GGCACTTTAT 
AGTCAGGAGC TCCGACTGGC AGGTTATTTA GCCAGATTGA CAAGGGTACC AATGGGGATG 
TTGCACAGGA TTCTGAATTT GTTGCTGCCT TCCAGTTCGC AAAGTGAGGT TCTTTCTATC 
CTTGATTCCA TTGGTGTATA TTCAGATGGT GCTGCTGAGA GTGATAGTAA TGCAGATGAG 
TCTGGCAGCT GTAAAAAAAG GAGGAACCAA GAGTGTTGGC AAGCATTGGA GTACAAAGTA 
AGGGAAGATC TGGTAACCAA AAGTTTGGAA AGGATCCTTT CTCTAAATAG GAGAAAAGAC 
AGCTTGATAA AGAAGAAGCA ACTTGCCCTT CTGGAAAGAC CATCATTCGT TCACCTTGAA 
AGTTTGCCTA CACGTCCTCC TGTGGAACGG TCCAGTCCAC ATGCTTCTTT TAACAGTGTG 
ACAGCTGAAG CCATCGAGGT ATTAGACACA GGGGAAAAGA TATTTTTGTT TCGTGACCGG 
AGTGATCCAA CACTTTCTCT TCATAACTAT CCGAGAAAAA AGAAGAAGAA CTTTCTTAAT 
TCCAAAAAGG CTGCTCGTGC AAATATCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015141282.1
CDS	16..3228
Translation	MDAKAEARSGSGQRGAPRAGPVPCGAGRLLAGLLVVLLSSAPAQPLGAAAWCPTQGHGVPGPPRGHCGRTAAGVITDDPWLQKEMNCLRMTALQLPVVVQASEVPDLILTSAASEDQWSHSIFALIPSWTRKILFKRESSVNHQLSFPLPPQPEDVSNLSASSGFGITLQKCAVVDVEHDPQTAYIRLLINNTNSLLSRNITDLILLDNITGLHVQKDSGNETRDGMQIYRKRFLQVGEYFAINYTALLDAKKTWKGRVLTLPAQLTFRSSSQNQTDLVSLTTLFTITEEEKTEVSYSHAIHASGFFIAFFVSLVLTCAVFFILYRTQLLRWSFYSENQEIQHELSQNHKLEHSQFSSGDLFSEDVIMNDQIIDILTFEESGNMLQALEEAGHLHESHLGMLLDRAEAEINSVKQKFNHDLKQEKQKLQQKLITKRKWKMLQKKEHWKEQLSIGNPFKTSREITDYLSHWKTVLSDHAIEFEELTEKLDNEGSEELRDLIFNLAEKTVEELKLVQYGVFVQELIKLSVPKMFLLEVVEEHKKEFIVKHEHLEREEREKSMAAEELLQLTRQKLSQELESSLSQQKKLRNWEQSVFMQLLTLPLSLSEEELLKMRQEFHCCFSQLDSSLALPKIRARALLQAFELEWRDAQLLKVSQNLAMTNKQLHSKTKKTGSKNRNKIDVLKKSLQDKIFIYEDSVKVENLTKVNYELLLEKDCQIHDLENKLGEYIASLEFQKSVKKCKMLELYAAVISVQGLLLENLSMSKTLSKSECIQILEAHNPEIEELDRKLEYEMLHKESAQQQQHLMNKQRWTPDGLRPSSEVVETNVDRQVTFLLRQAMNRCMQFINLHRQSLRDEQWNCMVLEDLLENIEMDAFLALYSQELRLAGYLARLTRVPMGMLHRILNLLLPSSSQSEVLSILDSIGVYSDGAAESDSNADESGSCKKRRNQECWQALEYKVREDLVTKSLERILSLNRRKDSLIKKKQLALLERPSFVHLESLPTRPPVERSSPHASFNSVTAEAIEVLDTGEKIFLFRDRSDPTLSLHNYPRKKKKNFLNSKKAARANIN

Target ORF information:

RefSeq Version	XM_015285796.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus EvC ciliary complex subunit 2 (EVC2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285796.2

ORF Insert Sequence:

ATGGATGCGA AGGCTGAGGC CCGGTCTGGA TCGGGGCAGC GCGGCGCTCC GCGGGCCGGC 
CCGGTGCCGT GCGGGGCTGG GCGGCTCCTG GCCGGGCTGC TCGTGGTACT GCTGTCCTCC 
GCGCCCGCTC AGCCCCTCGG GGCGGCTGCC TGGTGCCCCA CGCAGGGACA CGGGGTGCCG 
GGCCCACCCC GGGGGCACTG CGGGAGGACG GCGGCTGGGG TTATTACAGA TGATCCGTGG 
CTTCAAAAAG AGATGAATTG TCTGCGGATG ACAGCACTGC AGTTGCCTGT GGTGGTTCAA 
GCAAGTGAAG TCCCTGATCT GATCTTGACT TCTGCAGCCT CGGAAGATCA GTGGAGCCAT 
TCAATATTTG CTCTGATACC CTCATGGACA AGGAAGATTT TGTTCAAACG AGAGTCTTCT 
GTTAACCACC AGCTGTCTTT TCCCCTTCCA CCACAGCCTG AAGATGTGTC CAACTTGTCA 
GCATCCTCTG GATTTGGAAT AACCCTTCAG AAATGTGCTG TGGTGGATGT TGAACATGAC 
CCTCAAACTG CATACATTAG ACTTCTGATT AATAACACCA ACTCACTGCT CTCAAGAAAC 
ATTACTGATC TTATCCTTCT GGACAATATC ACCGGTCTAC ATGTTCAAAA AGATAGTGGT 
AATGAGACAA GAGATGGGAT GCAAATTTAC AGGAAAAGAT TCTTGCAAGT TGGGGAATAT 
TTTGCAATCA ACTATACTGC ACTTCTTGAT GCAAAGAAAA CGTGGAAAGG TAGGGTCTTG 
ACACTGCCTG CACAGCTGAC TTTCAGGAGT TCATCACAGA ATCAAACAGA TCTTGTATCA 
TTGACCACAT TGTTCACAAT AACTGAAGAA GAAAAGACCG AGGTCTCCTA CAGCCATGCC 
ATCCATGCTT CAGGGTTCTT CATTGCTTTT TTTGTTTCCC TGGTATTGAC ATGTGCTGTT 
TTCTTCATTC TTTACCGAAC CCAATTATTA AGATGGAGTT TCTATAGTGA AAACCAGGAA 
ATACAACATG AACTCAGTCA AAATCACAAA CTGGAGCATT CTCAGTTCAG TTCAGGTGAC 
CTCTTCAGTG AAGATGTTAT TATGAATGAC CAAATCATTG ATATACTGAC CTTTGAAGAG 
TCGGGGAATA TGCTTCAGGC TTTAGAAGAG GCGGGTCATC TACATGAAAG TCATTTGGGA 
ATGCTGCTGG ACCGAGCTGA GGCTGAAATT AATTCTGTTA AACAAAAATT CAATCATGAT 
TTAAAACAAG AAAAGCAAAA GCTTCAGCAG AAACTCATTA CCAAGCGAAA GTGGAAAATG 
TTGCAAAAGA AAGAGCATTG GAAGGAGCAG CTGTCCATAG GGAACCCCTT CAAAACCTCA 
AGGGAGATCA CTGACTACCT GAGCCACTGG AAAACTGTTC TGAGTGATCA TGCCATTGAA 
TTTGAAGAAC TTACTGAAAA GCTGGATAAT GAAGGCAGTG AAGAGCTGAG AGACCTTATA 
TTTAATCTAG CAGAGAAAAC TGTTGAAGAA CTTAAACTTG TTCAATATGG AGTATTTGTG 
CAAGAACTGA TAAAGCTCAG TGTGCCAAAG ATGTTCCTGC TGGAAGTTGT AGAGGAGCAT 
AAAAAAGAAT TCATTGTTAA ACATGAACAC CTTGAAAGGG AAGAACGTGA AAAGAGCATG 
GCTGCTGAAG AGTTGCTTCA GCTCACCAGG CAAAAACTAA GCCAAGAGCT GGAAAGTAGT 
CTTTCACAAC AGAAAAAATT AAGGAACTGG GAACAATCAG TATTCATGCA GCTTCTAACT 
TTGCCCCTTT CACTGTCTGA AGAGGAATTG CTCAAAATGA GGCAAGAGTT CCACTGCTGC 
TTTTCACAGC TGGACAGCAG TTTGGCTCTG CCCAAGATAA GAGCAAGAGC TTTACTTCAG 
GCTTTTGAGC TGGAGTGGAG GGATGCGCAG CTGCTGAAAG TCAGTCAGAA TCTAGCAATG 
ACCAATAAGC AGCTGCATTC TAAAACGAAA AAAACAGGAT CCAAGAATCG AAATAAGATT 
GATGTTTTGA AGAAATCTTT ACAGGACAAA ATTTTCATTT ATGAAGACTC AGTGAAAGTT 
GAAAACTTGA CTAAGGTCAA TTATGAGTTG CTACTTGAGA AAGACTGTCA GATACATGAT 
CTGGAAAATA AACTTGGAGA ATATATTGCT TCTTTAGAGT TTCAAAAGAG TGTTAAAAAA 
TGCAAAATGT TGGAGCTCTA TGCAGCAGTC ATAAGTGTGC AAGGTTTGCT CTTGGAAAAC 
CTGAGCATGT CAAAAACGCT GTCAAAATCG GAATGTATTC AGATTTTAGA AGCTCATAAT 
CCGGAGATTG AAGAACTTGA CAGGAAGCTG GAGTATGAAA TGCTGCATAA AGAGTCAGCA 
CAACAGCAGC AACATCTAAT GAATAAGCAG AGGTGGACAC CGGATGGATT GAGACCTTCA 
AGTGAAGTCG TAGAAACAAA TGTAGACAGA CAGGTGACAT TTCTGCTAAG ACAGGCCATG 
AATAGATGTA TGCAGTTTAT AAATCTGCAC CGGCAAAGCT TGAGAGATGA GCAATGGAAT 
TGTATGGTGC TTGAAGATCT CTTGGAAAAT ATAGAAATGG ATGCATTTTT GGCACTTTAT 
AGTCAGGAGC TCCGACTGGC AGGTTATTTA GCCAGATTGA CAAGGGTACC AATGGGGATG 
TTGCACAGGA TTCTGAATTT GTTGCTGCCT TCCAGTTCGC AAAGTGAGGT TCTTTCTATC 
CTTGATTCCA TTGGTGTATA TTCAGATGGT GCTGCTGAGA GTGATAGTAA TGCAGATGAG 
TCTGGCAGCT GTAAAAAAAG GAGGAACCAA GAGTGTTGGC AAGCATTGGA GTACAAAGTA 
AGGGAAGATC TGGTAACCAA AAGTTTGGAA AGGATCCTTT CTCTAAATAG GAGAAAAGAC 
AGCTTGATAA AGAAGAAGCA ACTTGCCCTT CTGGAAAGAC CATCATTCGT TCACCTTGAA 
AGTTTGCCTA CACGTCCTCC TGTGGAACGG TCCAGTCCAC ATGCTTCTTT TAACAGTGTG 
ACAGCTGAAG CCATCGAGGT ATTAGACACA GGGGAAAAGA TATTTTTGTT TCGTGACCGG 
AGTGATCCAA CACTTTCTCT TCATAACTAT CCGAGAAAAA AGAAGAAGAA CTTTCTTAAT 
TCCAAAAAGG CTGCTCGTGC AAATATCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	EVC2
Entrez Gene ID	770095
Full Name	EvC ciliary complex subunit 2
Synonyms	limbin
General protein information	Preferred Names EvC ciliary complex subunit 2 Names limbin Ellis van Creveld syndrome 2 (limbin) Ellis-van Creveld syndrome 2-like protein
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Homo sapiens (human)	EVC2	NP_667338.3
Pan troglodytes (chimpanzee)	EVC2	XP_001155684.1
Canis lupus familiaris (dog)	EVC2	XP_005618640.1
Bos taurus (cattle)	EVC2	NP_776352.1
Xenopus tropicalis (tropical clawed frog)	evc2	XP_002939100.2
Mus musculus (house mouse)	Evc2	NP_666032.1
Rattus norvegicus (Norway rat)	Evc2	NP_001099482.1
Gallus gallus (chicken)	EVC2	NP_001073217.1
	EVC2	XP_001118862.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

EVC2 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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