COL5A2 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL5A2
Entrez Gene ID	423986
Full Name	collagen type V alpha 2 chain
General protein information	Preferred Names collagen type V alpha 2 chain Names collagen alpha-2(V) chain alpha 2 type V collagen collagen, type V, alpha 2
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015289202.2	XP_015144688.1	collagen alpha-2(V) chain isoform X1
XM_015289203.2	XP_015144689.1	collagen alpha-2(V) chain isoform X3
XM_015289202.1	XP_015144688.1	collagen alpha-2(V) chain isoform X1
XM_015289203.1	XP_015144689.1	collagen alpha-2(V) chain isoform X3
XM_004942396.1	XP_004942453.1	collagen alpha-2(V) chain isoform X2
XM_004942396.2	XP_004942453.1	collagen alpha-2(V) chain isoform X2
XM_025152272.1	XP_025008040.1	collagen alpha-2(V) chain isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	COL5A2	NP_000384.2
Pan troglodytes (chimpanzee)	COL5A2	XP_001164152.1
Canis lupus familiaris (dog)	COL5A2	XP_005640450.1
Rattus norvegicus (Norway rat)	Col5a2	NP_445940.1
Xenopus tropicalis (tropical clawed frog)	col5a2	XP_002931546.2
Danio rerio (zebrafish)	col5a2a	NP_001139254.1
Gallus gallus (chicken)	COL5A2	XP_004942453.1
	COL5A2	XP_002799008.1
Mus musculus (house mouse)	Col5a2	NP_031763.2

Gene Ontology

The following COL5A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL5A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa10658	XM_004942396.2 Latest version!	Gallus gallus collagen type V alpha 2 chain (COL5A2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OGa38195	XM_015289202.2 Latest version!	Gallus gallus collagen type V alpha 2 chain (COL5A2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OGa38196	XM_015289203.2 Latest version!	Gallus gallus collagen type V alpha 2 chain (COL5A2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OGa38196	XM_025152272.1 Latest version!	Gallus gallus collagen type V alpha 2 chain (COL5A2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
View Old Accession Versions >>
OGa38195	XM_015289202.1	Gallus gallus collagen, type V, alpha 2 (COL5A2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OGa38196	XM_015289203.1	Gallus gallus collagen, type V, alpha 2 (COL5A2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OGa10658	XM_004942396.1	Gallus gallus collagen, type V, alpha 2 (COL5A2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL5A2 ( XM_004942396.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004942453.1 Gallus gallus collagen, type V, alpha 2 (COL5A2), transcript variant X2, mRNA.

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CloneID	OGa10658
Clone ID Related Accession (Same CDS sequence)	XM_004942396.1 , XM_004942396.2
Accession Version	XM_004942396.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	collagen alpha-2(V) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455901.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCAA TCAGGGTAGA AATGAGAACT TTTTTCATCC TTGCCGCTTT ATTAGGGCTA 
TTCGTCACCA TAAAAGCACA AGAAGAGGAA GATTACGATG CTGGAGAAGA TGTTGAAGAG 
ATTGCCTGCA CTCAGAATGG CCAGATGTAC CTGAACAGGG ATATTTGGAA GCCCTCACCG 
TGCCAGATTT GTGTCTGTGA CAACGGAGCT ATTCTTTGCG ATGAAATCCA GTGTCAAGAT 
GTGTTGGAGT GTGAGAACCC ACAGGTGCCC CCAGGAGAGT GCTGTCCAGT GTGTCCCCAT 
ACCGCACGTG ATTTTGACCC TACCGTTGGC AGAGGAAGAA AGGGGCAAAA AGGAGAACCT 
GGAATAGTGC CACCTGTTTC AGGAATACGA GGCCGCCCAG GGCCTGCAGG ACCTCCCGGT 
TCACAGGGAC CACGGGGAGA ACGAGGACCT AAGGGAAGAC CTGGTCCTCG TGGTCCTCAA 
GGGATTGATG GAGAACCCGG TATCCCTGGC CAGCCTGGTG ACCCTGGTCC TCCTGGGCAT 
CCAACTCACA CAGGACCAGA TGGGCTAAGC AGGCCATTTG CAGCTCAGAT GGCAGGTCTG 
GATGAAAAAT CAGGACTTGG TAGTCAGATG GGGCTGATGC CTGGTGCTGT GGGTCCAGTG 
GGTCCAAGAG GTCCTCAAGG TCTTCAAGGA AAACAAGGTG GTGCTGGCCC CACAGGCCCT 
CCTGGTGAAC CTGGAGATCC AGGACCTATG GGTCCAGTTG GTTCACGTGG ACCAGAAGGG 
CCTCCAGGCA AACCTGGTGA AGATGGTGAA CCCGGTAGAC CAGGACAGTC TGGTGAGCCT 
GGATTTCCAG GATCTCCAGG GCCTCGAGGC TTTCCTGGTG CTCCAGGTCT TCCAGGTCTC 
AAGGGTCACA GGGGATTGAA AGGTCTCGAT GGTCCAAAAG GTGAAATTGG AGCAGCTGGA 
GCAAAGGGAG AATCTGGACC AACAGGTGCC ATGGGTGCAA CAGGTCCCCT AGGACCTAGA 
GGAATGCCAG GAGAAAGAGG TCGAATTGGA CCACAGGGTG CCCCTGGTGG ACGTGGTCAG 
CATGGCATGC CTGGAAAGCC CGGGCCAATG GGTCCCCTTG GCATAACTGG TTCTCCAGGT 
TTTCCAGGCG CTCCTGGTAT GAAGGGAGAA GCAGGTCCAA CCGGTCCTCG TGGTCCTGAA 
GGCCCTCAAG GACAAAGAGG TGAAACTGGT CCACAAGGCC CAGCTGGATC ACAAGGTCTT 
CCTGGTACGG AAGGGACAGA TGGCTCTCCA GGTGCCAAAG GCCCAACTGG ATCTCCAGGC 
ACTGGGGGCC CCCCTGGCTT ATTGGGTCCA CCTGGTTCCC CAGGACCACA AGGCAGTACA 
GGCCCACCTG GTATTCGAGG GCAGCCGGGA GATCCTGGAG TTCCAGGTTT CAAGGGAGAA 
GCAGGACCCA AAGGTGAACC AGGCCCGCAA GGTCCCCAAG GTCCCATTGG CCCCATAGGT 
GAAGAAGGGA AAAGAGGCCC TCGAGGTGAT CCAGGGTCAG TGGGTCCACC AGGTCCAGTT 
GGAGAGAGGG GTGCTCCTGG GAACCGTGGT TTTCCAGGTT CTGATGGCTT GCCTGGACCA 
AAGGGTGCCC AAGGAGAGCG TGGTCCAGCA GGTTCTTCTG GTCCTAAAGG AGGTCAGGGA 
GATCCTGGGC GTCCTGGTGA GCCTGGACTC CCAGGTGCAA GGGGTTTGAC AGGAAATCCA 
GGTGTTCAAG GCCCTGAAGG AAAACTTGGC CCCTTGGGTG GCCCTGGAGA AGATGGACGT 
CCTGGTCCTG CAGGTTCGAT AGGAATCAGA GGTCAGCCAG GCAGTATGGG CCTTTCTGGG 
CCAAAGGGTA GTAGTGGTGA TCCTGGTAAA CCAGGAGAAG CAGGAAATGC AGGTGTTCCA 
GGACAAAGAG GAGCTCCTGG CAAAGACGGT GAAGTCGGTC CTTCTGGTCC TGTTGGCCCA 
CCAGGCCTGG CAGGAGAAAG AGGAGAACAA GGCCCTCCAG GTCCTACAGG GTTTCAGGGC 
TTACCTGGGC CTCCAGGTCC TCCTGGTGAA GGAGGAAAGC CAGGTGATCA GGGTGTTCCC 
GGAGATCCTG GAGTTGTTGG TCCATTGGGT CCCAGGGGAG AACGTGGCAA CCCGGGGGAA 
CGGGGAGAGC CAGGTGCATC AGGACTTGCA GGTGAAAAGG GTATGGCTGG AGGTCATGGT 
CCTGATGGGC CAAAGGGAAG CCCAGGCCCT AGTGGGACGC CTGGTGATCC AGGCCCACCA 
GGTCTTCAAG GTATGCCTGG AGAAAGAGGG ATTGCTGGAA CTCCTGGTCC AAAGGGTGAC 
AGAGGTGGTA TGGGTGAGAA AGGTGCTGAA GGTACTGCTG GCAATGATGG GGCAAGAGGT 
CTTCCTGGTC CAATAGGCCC AATGGGTCCA GCAGGGTCCA CTGGAGAAAA GGGTGAACCA 
GGTCCTCGAG GTCTTGTTGG TCCTCCCGGT TCTCGTGGAA ACCCTGGTTC TCGAGGAGAA 
AATGGCCCAA CTGGTGCTGT TGGTTTTGCT GGTCCTCCAG GCCCTGATGG TCAGCCGGGT 
GTGAAAGGTG AACCTGGGGA ACCTGGACAG AAAGGAGATG CTGGATCTCC AGGACCACAG 
GGTCTTGCTG GATCTCCTGG TCCACCAGGT CCTTCAGGTG TTCCTGGTCT GAAAGGTGGT 
CGAGGAACCC AGGGTCCCCC TGGTGCTACT GGATTCCCTG GATCTGCTGG AAGAGTTGGA 
CCTCCTGGAC CCACTGGAGC TCCAGGGCCT GCTGGGCCTA TTGGTGAGCC AGGAAAAGAA 
GGTCCCCCAG GTCTTCGTGG TGATCCTGGT TCTCATGGTC GTGTGGGTGA TCGAGGACCA 
GCTGGGCCCC CTGGTGGTCC TGGAGACAAG GGTGATGCAG GAGAAGATGG GCAACCGGGT 
CCAGATGGCC CCCCAGGTCC AGCAGGAACT ACTGGTCAAA GAGGTATTGT TGGTATGCCA 
GGACAGCGAG GGGAAAGAGG CATGCCAGGA CTGCCAGGTC CTGCAGGTAC ACCAGGAAAA 
CAAGGTCCCA CAGGGCCACC CGGTGATAAA GGTCCCCCAG GACCTATTGG CCAATCAGGT 
GCCACTGGAC CTGTAGGGGA AGCTGGTCCA GAAGGACCTG CTGGTAATGA TGGTACTCCA 
GGACGAGATG GAGCTGTTGG AGAGCGTGGT GATCGTGGTG AACCTGGCCC TGCAGGCCTA 
CCTGGTTCTC CAGGAGGTCC TGGAACTCCA GGTCCTGTTG GCCCCCCGGG AGATGCAGGT 
CAAAGAGGTG AAACTGGTTC TCGAGGGCCA GTGGGTCCCC CAGGCCGTGC AGGAAAAAGA 
GGTTTGCCTG GTCCCCAAGG GCCTCGTGGT GACAAAGGTG ACAATGGAGA TCGAGGTGAC 
AGGGGCCAGA AAGGACACAG AGGTTTCACT GGGCTTCAAG GTCTTCCTGG TCCTCCTGGC 
CCTATTGGTG AACAGGGCAG TCCTGGCATT CCAGGCCCAT TTGGTCCTCG GGGTCCTCCA 
GGTCCAGTTG GGCCTTCAGG CAAAGATGGA AGTCCTGGAC CACTAGGGCC GATTGGGCCT 
CCTGGTGTTA GAGGAAGTGT AGGAGAAGCT GGGCCTGAGG GTCCACCAGG TGATCCTGGC 
CCTCCTGGTC CCCCCGGCCC TCCAGGTCAC CTCACTGCTG CCATTGGTGA CATTATGGGA 
CACTATGATG ATGCTATGGC AGATCCACTA CCAGAGTTTA CTGAAGATGA AGCAGCTCCT 
GATGACCACA ATAAAACAGA CCCAGGAGTC CATGCCACGC TGAAGTCACT GAGCAGCCAG 
ATTGAGACTA TGCGCAGCCC AGATGGTTCG AAGAAACACC CAGCACGGAC TTGTGATGAC 
CTGAAGCTGT GCCACCCATC CAAGAAGAGT GGTGAATATT GGATTGATCC TAATCAGGGA 
TGTGTTGAAG ATGCTATAAA AGTATACTGC AACATGGAAA CTGGAGAGAC CTGCATTTCT 
GCAAATCCAT CAACTATCCC ACGTAAAACA TGGTGGGCCA GCAGATCATC TGACTTGAAG 
CCCATTTGGT ATGGCCTTGA TATGAACAGG GGATCACAGT TTGTGTATGG GGATACAGCT 
GTCACTCAAA TGACATTCCT GCGCCTCCTG TCAAAGGAAG CTTCCCAGAA TATCACTTAC 
CTCTGTAAGA ACAGTGTTGG GTACATGGAT GACCAAACTA AGAACCTCAA GAAAGCTGTC 
ATTCTGAAAG GGGCCAATGA CCTGGAGATC AAGGCGGAGG GAAACAGCCG ATTCAGATAC 
ACTGTTCTTC AAGACAGCTG TTCTAAACGT AATGGCAACG TGGGCAAGAC GGTCTTTGAG 
TACCGAACGC AGAACGTGGC ACGGTTGCCT ATCATAGATA TCGCACCAGT GGATGTTGGC 
AGCACTGACC AGGAATTTGG AATTGAAATT GGGCCAGTTT GTTTTGTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004942453.1
CDS	477..4967
Translation	MMAIRVEMRTFFILAALLGLFVTIKAQEEEDYDAGEDVEEIACTQNGQMYLNRDIWKPSPCQICVCDNGAILCDEIQCQDVLECENPQVPPGECCPVCPHTARDFDPTVGRGRKGQKGEPGIVPPVSGIRGRPGPAGPPGSQGPRGERGPKGRPGPRGPQGIDGEPGIPGQPGDPGPPGHPTHTGPDGLSRPFAAQMAGLDEKSGLGSQMGLMPGAVGPVGPRGPQGLQGKQGGAGPTGPPGEPGDPGPMGPVGSRGPEGPPGKPGEDGEPGRPGQSGEPGFPGSPGPRGFPGAPGLPGLKGHRGLKGLDGPKGEIGAAGAKGESGPTGAMGATGPLGPRGMPGERGRIGPQGAPGGRGQHGMPGKPGPMGPLGITGSPGFPGAPGMKGEAGPTGPRGPEGPQGQRGETGPQGPAGSQGLPGTEGTDGSPGAKGPTGSPGTGGPPGLLGPPGSPGPQGSTGPPGIRGQPGDPGVPGFKGEAGPKGEPGPQGPQGPIGPIGEEGKRGPRGDPGSVGPPGPVGERGAPGNRGFPGSDGLPGPKGAQGERGPAGSSGPKGGQGDPGRPGEPGLPGARGLTGNPGVQGPEGKLGPLGGPGEDGRPGPAGSIGIRGQPGSMGLSGPKGSSGDPGKPGEAGNAGVPGQRGAPGKDGEVGPSGPVGPPGLAGERGEQGPPGPTGFQGLPGPPGPPGEGGKPGDQGVPGDPGVVGPLGPRGERGNPGERGEPGASGLAGEKGMAGGHGPDGPKGSPGPSGTPGDPGPPGLQGMPGERGIAGTPGPKGDRGGMGEKGAEGTAGNDGARGLPGPIGPMGPAGSTGEKGEPGPRGLVGPPGSRGNPGSRGENGPTGAVGFAGPPGPDGQPGVKGEPGEPGQKGDAGSPGPQGLAGSPGPPGPSGVPGLKGGRGTQGPPGATGFPGSAGRVGPPGPTGAPGPAGPIGEPGKEGPPGLRGDPGSHGRVGDRGPAGPPGGPGDKGDAGEDGQPGPDGPPGPAGTTGQRGIVGMPGQRGERGMPGLPGPAGTPGKQGPTGPPGDKGPPGPIGQSGATGPVGEAGPEGPAGNDGTPGRDGAVGERGDRGEPGPAGLPGSPGGPGTPGPVGPPGDAGQRGETGSRGPVGPPGRAGKRGLPGPQGPRGDKGDNGDRGDRGQKGHRGFTGLQGLPGPPGPIGEQGSPGIPGPFGPRGPPGPVGPSGKDGSPGPLGPIGPPGVRGSVGEAGPEGPPGDPGPPGPPGPPGHLTAAIGDIMGHYDDAMADPLPEFTEDEAAPDDHNKTDPGVHATLKSLSSQIETMRSPDGSKKHPARTCDDLKLCHPSKKSGEYWIDPNQGCVEDAIKVYCNMETGETCISANPSTIPRKTWWASRSSDLKPIWYGLDMNRGSQFVYGDTAVTQMTFLRLLSKEASQNITYLCKNSVGYMDDQTKNLKKAVILKGANDLEIKAEGNSRFRYTVLQDSCSKRNGNVGKTVFEYRTQNVARLPIIDIAPVDVGSTDQEFGIEIGPVCFV

Target ORF information:

RefSeq Version	XM_004942396.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen, type V, alpha 2 (COL5A2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004942396.1

ORF Insert Sequence:

ATGATGGCAA TCAGGGTAGA AATGAGAACT TTTTTCATCC TTGCCGCTTT ATTAGGGCTA 
TTCGTCACCA TAAAAGCACA AGAAGAGGAA GATTACGATG CTGGAGAAGA TGTTGAAGAG 
ATTGCCTGCA CTCAGAATGG CCAGATGTAC CTGAACAGGG ATATTTGGAA GCCCTCACCG 
TGCCAGATTT GTGTCTGTGA CAACGGAGCT ATTCTTTGCG ATGAAATCCA GTGTCAAGAT 
GTGTTGGAGT GTGAGAACCC ACAGGTGCCC CCAGGAGAGT GCTGTCCAGT GTGTCCCCAT 
ACCGCACGTG ATTTTGACCC TACCGTTGGC AGAGGAAGAA AGGGGCAAAA AGGAGAACCT 
GGAATAGTGC CACCTGTTTC AGGAATACGA GGCCGCCCAG GGCCTGCAGG ACCTCCCGGT 
TCACAGGGAC CACGGGGAGA ACGAGGACCT AAGGGAAGAC CTGGTCCTCG TGGTCCTCAA 
GGGATTGATG GAGAACCCGG TATCCCTGGC CAGCCTGGTG ACCCTGGTCC TCCTGGGCAT 
CCAACTCACA CAGGACCAGA TGGGCTAAGC AGGCCATTTG CAGCTCAGAT GGCAGGTCTG 
GATGAAAAAT CAGGACTTGG TAGTCAGATG GGGCTGATGC CTGGTGCTGT GGGTCCAGTG 
GGTCCAAGAG GTCCTCAAGG TCTTCAAGGA AAACAAGGTG GTGCTGGCCC CACAGGCCCT 
CCTGGTGAAC CTGGAGATCC AGGACCTATG GGTCCAGTTG GTTCACGTGG ACCAGAAGGG 
CCTCCAGGCA AACCTGGTGA AGATGGTGAA CCCGGTAGAC CAGGACAGTC TGGTGAGCCT 
GGATTTCCAG GATCTCCAGG GCCTCGAGGC TTTCCTGGTG CTCCAGGTCT TCCAGGTCTC 
AAGGGTCACA GGGGATTGAA AGGTCTCGAT GGTCCAAAAG GTGAAATTGG AGCAGCTGGA 
GCAAAGGGAG AATCTGGACC AACAGGTGCC ATGGGTGCAA CAGGTCCCCT AGGACCTAGA 
GGAATGCCAG GAGAAAGAGG TCGAATTGGA CCACAGGGTG CCCCTGGTGG ACGTGGTCAG 
CATGGCATGC CTGGAAAGCC CGGGCCAATG GGTCCCCTTG GCATAACTGG TTCTCCAGGT 
TTTCCAGGCG CTCCTGGTAT GAAGGGAGAA GCAGGTCCAA CCGGTCCTCG TGGTCCTGAA 
GGCCCTCAAG GACAAAGAGG TGAAACTGGT CCACAAGGCC CAGCTGGATC ACAAGGTCTT 
CCTGGTACGG AAGGGACAGA TGGCTCTCCA GGTGCCAAAG GCCCAACTGG ATCTCCAGGC 
ACTGGGGGCC CCCCTGGCTT ATTGGGTCCA CCTGGTTCCC CAGGACCACA AGGCAGTACA 
GGCCCACCTG GTATTCGAGG GCAGCCGGGA GATCCTGGAG TTCCAGGTTT CAAGGGAGAA 
GCAGGACCCA AAGGTGAACC AGGCCCGCAA GGTCCCCAAG GTCCCATTGG CCCCATAGGT 
GAAGAAGGGA AAAGAGGCCC TCGAGGTGAT CCAGGGTCAG TGGGTCCACC AGGTCCAGTT 
GGAGAGAGGG GTGCTCCTGG GAACCGTGGT TTTCCAGGTT CTGATGGCTT GCCTGGACCA 
AAGGGTGCCC AAGGAGAGCG TGGTCCAGCA GGTTCTTCTG GTCCTAAAGG AGGTCAGGGA 
GATCCTGGGC GTCCTGGTGA GCCTGGACTC CCAGGTGCAA GGGGTTTGAC AGGAAATCCA 
GGTGTTCAAG GCCCTGAAGG AAAACTTGGC CCCTTGGGTG GCCCTGGAGA AGATGGACGT 
CCTGGTCCTG CAGGTTCGAT AGGAATCAGA GGTCAGCCAG GCAGTATGGG CCTTTCTGGG 
CCAAAGGGTA GTAGTGGTGA TCCTGGTAAA CCAGGAGAAG CAGGAAATGC AGGTGTTCCA 
GGACAAAGAG GAGCTCCTGG CAAAGACGGT GAAGTCGGTC CTTCTGGTCC TGTTGGCCCA 
CCAGGCCTGG CAGGAGAAAG AGGAGAACAA GGCCCTCCAG GTCCTACAGG GTTTCAGGGC 
TTACCTGGGC CTCCAGGTCC TCCTGGTGAA GGAGGAAAGC CAGGTGATCA GGGTGTTCCC 
GGAGATCCTG GAGTTGTTGG TCCATTGGGT CCCAGGGGAG AACGTGGCAA CCCGGGGGAA 
CGGGGAGAGC CAGGTGCATC AGGACTTGCA GGTGAAAAGG GTATGGCTGG AGGTCATGGT 
CCTGATGGGC CAAAGGGAAG CCCAGGCCCT AGTGGGACGC CTGGTGATCC AGGCCCACCA 
GGTCTTCAAG GTATGCCTGG AGAAAGAGGG ATTGCTGGAA CTCCTGGTCC AAAGGGTGAC 
AGAGGTGGTA TGGGTGAGAA AGGTGCTGAA GGTACTGCTG GCAATGATGG GGCAAGAGGT 
CTTCCTGGTC CAATAGGCCC AATGGGTCCA GCAGGGTCCA CTGGAGAAAA GGGTGAACCA 
GGTCCTCGAG GTCTTGTTGG TCCTCCCGGT TCTCGTGGAA ACCCTGGTTC TCGAGGAGAA 
AATGGCCCAA CTGGTGCTGT TGGTTTTGCT GGTCCTCCAG GCCCTGATGG TCAGCCGGGT 
GTGAAAGGTG AACCTGGGGA ACCTGGACAG AAAGGAGATG CTGGATCTCC AGGACCACAG 
GGTCTTGCTG GATCTCCTGG TCCACCAGGT CCTTCAGGTG TTCCTGGTCT GAAAGGTGGT 
CGAGGAACCC AGGGTCCCCC TGGTGCTACT GGATTCCCTG GATCTGCTGG AAGAGTTGGA 
CCTCCTGGAC CCACTGGAGC TCCAGGGCCT GCTGGGCCTA TTGGTGAGCC AGGAAAAGAA 
GGTCCCCCAG GTCTTCGTGG TGATCCTGGT TCTCATGGTC GTGTGGGTGA TCGAGGACCA 
GCTGGGCCCC CTGGTGGTCC TGGAGACAAG GGTGATGCAG GAGAAGATGG GCAACCGGGT 
CCAGATGGCC CCCCAGGTCC AGCAGGAACT ACTGGTCAAA GAGGTATTGT TGGTATGCCA 
GGACAGCGAG GGGAAAGAGG CATGCCAGGA CTGCCAGGTC CTGCAGGTAC ACCAGGAAAA 
CAAGGTCCCA CAGGGCCACC CGGTGATAAA GGTCCCCCAG GACCTATTGG CCAATCAGGT 
GCCACTGGAC CTGTAGGGGA AGCTGGTCCA GAAGGACCTG CTGGTAATGA TGGTACTCCA 
GGACGAGATG GAGCTGTTGG AGAGCGTGGT GATCGTGGTG AACCTGGCCC TGCAGGCCTA 
CCTGGTTCTC CAGGAGGTCC TGGAACTCCA GGTCCTGTTG GCCCCCCGGG AGATGCAGGT 
CAAAGAGGTG AAACTGGTTC TCGAGGGCCA GTGGGTCCCC CAGGCCGTGC AGGAAAAAGA 
GGTTTGCCTG GTCCCCAAGG GCCTCGTGGT GACAAAGGTG ACAATGGAGA TCGAGGTGAC 
AGGGGCCAGA AAGGACACAG AGGTTTCACT GGGCTTCAAG GTCTTCCTGG TCCTCCTGGC 
CCTATTGGTG AACAGGGCAG TCCTGGCATT CCAGGCCCAT TTGGTCCTCG GGGTCCTCCA 
GGTCCAGTTG GGCCTTCAGG CAAAGATGGA AGTCCTGGAC CACTAGGGCC GATTGGGCCT 
CCTGGTGTTA GAGGAAGTGT AGGAGAAGCT GGGCCTGAGG GTCCACCAGG TGATCCTGGC 
CCTCCTGGTC CCCCCGGCCC TCCAGGTCAC CTCACTGCTG CCATTGGTGA CATTATGGGA 
CACTATGATG ATGCTATGGC AGATCCACTA CCAGAGTTTA CTGAAGATGA AGCAGCTCCT 
GATGACCACA ATAAAACAGA CCCAGGAGTC CATGCCACGC TGAAGTCACT GAGCAGCCAG 
ATTGAGACTA TGCGCAGCCC AGATGGTTCG AAGAAACACC CAGCACGGAC TTGTGATGAC 
CTGAAGCTGT GCCACCCATC CAAGAAGAGT GGTGAATATT GGATTGATCC TAATCAGGGA 
TGTGTTGAAG ATGCTATAAA AGTATACTGC AACATGGAAA CTGGAGAGAC CTGCATTTCT 
GCAAATCCAT CAACTATCCC ACGTAAAACA TGGTGGGCCA GCAGATCATC TGACTTGAAG 
CCCATTTGGT ATGGCCTTGA TATGAACAGG GGATCACAGT TTGTGTATGG GGATACAGCT 
GTCACTCAAA TGACATTCCT GCGCCTCCTG TCAAAGGAAG CTTCCCAGAA TATCACTTAC 
CTCTGTAAGA ACAGTGTTGG GTACATGGAT GACCAAACTA AGAACCTCAA GAAAGCTGTC 
ATTCTGAAAG GGGCCAATGA CCTGGAGATC AAGGCGGAGG GAAACAGCCG ATTCAGATAC 
ACTGTTCTTC AAGACAGCTG TTCTAAACGT AATGGCAACG TGGGCAAGAC GGTCTTTGAG 
TACCGAACGC AGAACGTGGC ACGGTTGCCT ATCATAGATA TCGCACCAGT GGATGTTGGC 
AGCACTGACC AGGAATTTGG AATTGAAATT GGGCCAGTTT GTTTTGTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A2 ( XM_004942396.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004942453.1 Gallus gallus collagen type V alpha 2 chain (COL5A2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa10658
Clone ID Related Accession (Same CDS sequence)	XM_004942396.1 , XM_004942396.2
Accession Version	XM_004942396.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	collagen alpha-2(V) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004942396.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCAA TCAGGGTAGA AATGAGAACT TTTTTCATCC TTGCCGCTTT ATTAGGGCTA 
TTCGTCACCA TAAAAGCACA AGAAGAGGAA GATTACGATG CTGGAGAAGA TGTTGAAGAG 
ATTGCCTGCA CTCAGAATGG CCAGATGTAC CTGAACAGGG ATATTTGGAA GCCCTCACCG 
TGCCAGATTT GTGTCTGTGA CAACGGAGCT ATTCTTTGCG ATGAAATCCA GTGTCAAGAT 
GTGTTGGAGT GTGAGAACCC ACAGGTGCCC CCAGGAGAGT GCTGTCCAGT GTGTCCCCAT 
ACCGCACGTG ATTTTGACCC TACCGTTGGC AGAGGAAGAA AGGGGCAAAA AGGAGAACCT 
GGAATAGTGC CACCTGTTTC AGGAATACGA GGCCGCCCAG GGCCTGCAGG ACCTCCCGGT 
TCACAGGGAC CACGGGGAGA ACGAGGACCT AAGGGAAGAC CTGGTCCTCG TGGTCCTCAA 
GGGATTGATG GAGAACCCGG TATCCCTGGC CAGCCTGGTG ACCCTGGTCC TCCTGGGCAT 
CCAACTCACA CAGGACCAGA TGGGCTAAGC AGGCCATTTG CAGCTCAGAT GGCAGGTCTG 
GATGAAAAAT CAGGACTTGG TAGTCAGATG GGGCTGATGC CTGGTGCTGT GGGTCCAGTG 
GGTCCAAGAG GTCCTCAAGG TCTTCAAGGA AAACAAGGTG GTGCTGGCCC CACAGGCCCT 
CCTGGTGAAC CTGGAGATCC AGGACCTATG GGTCCAGTTG GTTCACGTGG ACCAGAAGGG 
CCTCCAGGCA AACCTGGTGA AGATGGTGAA CCCGGTAGAC CAGGACAGTC TGGTGAGCCT 
GGATTTCCAG GATCTCCAGG GCCTCGAGGC TTTCCTGGTG CTCCAGGTCT TCCAGGTCTC 
AAGGGTCACA GGGGATTGAA AGGTCTCGAT GGTCCAAAAG GTGAAATTGG AGCAGCTGGA 
GCAAAGGGAG AATCTGGACC AACAGGTGCC ATGGGTGCAA CAGGTCCCCT AGGACCTAGA 
GGAATGCCAG GAGAAAGAGG TCGAATTGGA CCACAGGGTG CCCCTGGTGG ACGTGGTCAG 
CATGGCATGC CTGGAAAGCC CGGGCCAATG GGTCCCCTTG GCATAACTGG TTCTCCAGGT 
TTTCCAGGCG CTCCTGGTAT GAAGGGAGAA GCAGGTCCAA CCGGTCCTCG TGGTCCTGAA 
GGCCCTCAAG GACAAAGAGG TGAAACTGGT CCACAAGGCC CAGCTGGATC ACAAGGTCTT 
CCTGGTACGG AAGGGACAGA TGGCTCTCCA GGTGCCAAAG GCCCAACTGG ATCTCCAGGC 
ACTGGGGGCC CCCCTGGCTT ATTGGGTCCA CCTGGTTCCC CAGGACCACA AGGCAGTACA 
GGCCCACCTG GTATTCGAGG GCAGCCGGGA GATCCTGGAG TTCCAGGTTT CAAGGGAGAA 
GCAGGACCCA AAGGTGAACC AGGCCCGCAA GGTCCCCAAG GTCCCATTGG CCCCATAGGT 
GAAGAAGGGA AAAGAGGCCC TCGAGGTGAT CCAGGGTCAG TGGGTCCACC AGGTCCAGTT 
GGAGAGAGGG GTGCTCCTGG GAACCGTGGT TTTCCAGGTT CTGATGGCTT GCCTGGACCA 
AAGGGTGCCC AAGGAGAGCG TGGTCCAGCA GGTTCTTCTG GTCCTAAAGG AGGTCAGGGA 
GATCCTGGGC GTCCTGGTGA GCCTGGACTC CCAGGTGCAA GGGGTTTGAC AGGAAATCCA 
GGTGTTCAAG GCCCTGAAGG AAAACTTGGC CCCTTGGGTG GCCCTGGAGA AGATGGACGT 
CCTGGTCCTG CAGGTTCGAT AGGAATCAGA GGTCAGCCAG GCAGTATGGG CCTTTCTGGG 
CCAAAGGGTA GTAGTGGTGA TCCTGGTAAA CCAGGAGAAG CAGGAAATGC AGGTGTTCCA 
GGACAAAGAG GAGCTCCTGG CAAAGACGGT GAAGTCGGTC CTTCTGGTCC TGTTGGCCCA 
CCAGGCCTGG CAGGAGAAAG AGGAGAACAA GGCCCTCCAG GTCCTACAGG GTTTCAGGGC 
TTACCTGGGC CTCCAGGTCC TCCTGGTGAA GGAGGAAAGC CAGGTGATCA GGGTGTTCCC 
GGAGATCCTG GAGTTGTTGG TCCATTGGGT CCCAGGGGAG AACGTGGCAA CCCGGGGGAA 
CGGGGAGAGC CAGGTGCATC AGGACTTGCA GGTGAAAAGG GTATGGCTGG AGGTCATGGT 
CCTGATGGGC CAAAGGGAAG CCCAGGCCCT AGTGGGACGC CTGGTGATCC AGGCCCACCA 
GGTCTTCAAG GTATGCCTGG AGAAAGAGGG ATTGCTGGAA CTCCTGGTCC AAAGGGTGAC 
AGAGGTGGTA TGGGTGAGAA AGGTGCTGAA GGTACTGCTG GCAATGATGG GGCAAGAGGT 
CTTCCTGGTC CAATAGGCCC AATGGGTCCA GCAGGGTCCA CTGGAGAAAA GGGTGAACCA 
GGTCCTCGAG GTCTTGTTGG TCCTCCCGGT TCTCGTGGAA ACCCTGGTTC TCGAGGAGAA 
AATGGCCCAA CTGGTGCTGT TGGTTTTGCT GGTCCTCCAG GCCCTGATGG TCAGCCGGGT 
GTGAAAGGTG AACCTGGGGA ACCTGGACAG AAAGGAGATG CTGGATCTCC AGGACCACAG 
GGTCTTGCTG GATCTCCTGG TCCACCAGGT CCTTCAGGTG TTCCTGGTCT GAAAGGTGGT 
CGAGGAACCC AGGGTCCCCC TGGTGCTACT GGATTCCCTG GATCTGCTGG AAGAGTTGGA 
CCTCCTGGAC CCACTGGAGC TCCAGGGCCT GCTGGGCCTA TTGGTGAGCC AGGAAAAGAA 
GGTCCCCCAG GTCTTCGTGG TGATCCTGGT TCTCATGGTC GTGTGGGTGA TCGAGGACCA 
GCTGGGCCCC CTGGTGGTCC TGGAGACAAG GGTGATGCAG GAGAAGATGG GCAACCGGGT 
CCAGATGGCC CCCCAGGTCC AGCAGGAACT ACTGGTCAAA GAGGTATTGT TGGTATGCCA 
GGACAGCGAG GGGAAAGAGG CATGCCAGGA CTGCCAGGTC CTGCAGGTAC ACCAGGAAAA 
CAAGGTCCCA CAGGGCCACC CGGTGATAAA GGTCCCCCAG GACCTATTGG CCAATCAGGT 
GCCACTGGAC CTGTAGGGGA AGCTGGTCCA GAAGGACCTG CTGGTAATGA TGGTACTCCA 
GGACGAGATG GAGCTGTTGG AGAGCGTGGT GATCGTGGTG AACCTGGCCC TGCAGGCCTA 
CCTGGTTCTC CAGGAGGTCC TGGAACTCCA GGTCCTGTTG GCCCCCCGGG AGATGCAGGT 
CAAAGAGGTG AAACTGGTTC TCGAGGGCCA GTGGGTCCCC CAGGCCGTGC AGGAAAAAGA 
GGTTTGCCTG GTCCCCAAGG GCCTCGTGGT GACAAAGGTG ACAATGGAGA TCGAGGTGAC 
AGGGGCCAGA AAGGACACAG AGGTTTCACT GGGCTTCAAG GTCTTCCTGG TCCTCCTGGC 
CCTATTGGTG AACAGGGCAG TCCTGGCATT CCAGGCCCAT TTGGTCCTCG GGGTCCTCCA 
GGTCCAGTTG GGCCTTCAGG CAAAGATGGA AGTCCTGGAC CACTAGGGCC GATTGGGCCT 
CCTGGTGTTA GAGGAAGTGT AGGAGAAGCT GGGCCTGAGG GTCCACCAGG TGATCCTGGC 
CCTCCTGGTC CCCCCGGCCC TCCAGGTCAC CTCACTGCTG CCATTGGTGA CATTATGGGA 
CACTATGATG ATGCTATGGC AGATCCACTA CCAGAGTTTA CTGAAGATGA AGCAGCTCCT 
GATGACCACA ATAAAACAGA CCCAGGAGTC CATGCCACGC TGAAGTCACT GAGCAGCCAG 
ATTGAGACTA TGCGCAGCCC AGATGGTTCG AAGAAACACC CAGCACGGAC TTGTGATGAC 
CTGAAGCTGT GCCACCCATC CAAGAAGAGT GGTGAATATT GGATTGATCC TAATCAGGGA 
TGTGTTGAAG ATGCTATAAA AGTATACTGC AACATGGAAA CTGGAGAGAC CTGCATTTCT 
GCAAATCCAT CAACTATCCC ACGTAAAACA TGGTGGGCCA GCAGATCATC TGACTTGAAG 
CCCATTTGGT ATGGCCTTGA TATGAACAGG GGATCACAGT TTGTGTATGG GGATACAGCT 
GTCACTCAAA TGACATTCCT GCGCCTCCTG TCAAAGGAAG CTTCCCAGAA TATCACTTAC 
CTCTGTAAGA ACAGTGTTGG GTACATGGAT GACCAAACTA AGAACCTCAA GAAAGCTGTC 
ATTCTGAAAG GGGCCAATGA CCTGGAGATC AAGGCGGAGG GAAACAGCCG ATTCAGATAC 
ACTGTTCTTC AAGACAGCTG TTCTAAACGT AATGGCAACG TGGGCAAGAC GGTCTTTGAG 
TACCGAACGC AGAACGTGGC ACGGTTGCCT ATCATAGATA TCGCACCAGT GGATGTTGGC 
AGCACTGACC AGGAATTTGG AATTGAAATT GGGCCAGTTT GTTTTGTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004942453.1
CDS	165..4655
Translation	MMAIRVEMRTFFILAALLGLFVTIKAQEEEDYDAGEDVEEIACTQNGQMYLNRDIWKPSPCQICVCDNGAILCDEIQCQDVLECENPQVPPGECCPVCPHTARDFDPTVGRGRKGQKGEPGIVPPVSGIRGRPGPAGPPGSQGPRGERGPKGRPGPRGPQGIDGEPGIPGQPGDPGPPGHPTHTGPDGLSRPFAAQMAGLDEKSGLGSQMGLMPGAVGPVGPRGPQGLQGKQGGAGPTGPPGEPGDPGPMGPVGSRGPEGPPGKPGEDGEPGRPGQSGEPGFPGSPGPRGFPGAPGLPGLKGHRGLKGLDGPKGEIGAAGAKGESGPTGAMGATGPLGPRGMPGERGRIGPQGAPGGRGQHGMPGKPGPMGPLGITGSPGFPGAPGMKGEAGPTGPRGPEGPQGQRGETGPQGPAGSQGLPGTEGTDGSPGAKGPTGSPGTGGPPGLLGPPGSPGPQGSTGPPGIRGQPGDPGVPGFKGEAGPKGEPGPQGPQGPIGPIGEEGKRGPRGDPGSVGPPGPVGERGAPGNRGFPGSDGLPGPKGAQGERGPAGSSGPKGGQGDPGRPGEPGLPGARGLTGNPGVQGPEGKLGPLGGPGEDGRPGPAGSIGIRGQPGSMGLSGPKGSSGDPGKPGEAGNAGVPGQRGAPGKDGEVGPSGPVGPPGLAGERGEQGPPGPTGFQGLPGPPGPPGEGGKPGDQGVPGDPGVVGPLGPRGERGNPGERGEPGASGLAGEKGMAGGHGPDGPKGSPGPSGTPGDPGPPGLQGMPGERGIAGTPGPKGDRGGMGEKGAEGTAGNDGARGLPGPIGPMGPAGSTGEKGEPGPRGLVGPPGSRGNPGSRGENGPTGAVGFAGPPGPDGQPGVKGEPGEPGQKGDAGSPGPQGLAGSPGPPGPSGVPGLKGGRGTQGPPGATGFPGSAGRVGPPGPTGAPGPAGPIGEPGKEGPPGLRGDPGSHGRVGDRGPAGPPGGPGDKGDAGEDGQPGPDGPPGPAGTTGQRGIVGMPGQRGERGMPGLPGPAGTPGKQGPTGPPGDKGPPGPIGQSGATGPVGEAGPEGPAGNDGTPGRDGAVGERGDRGEPGPAGLPGSPGGPGTPGPVGPPGDAGQRGETGSRGPVGPPGRAGKRGLPGPQGPRGDKGDNGDRGDRGQKGHRGFTGLQGLPGPPGPIGEQGSPGIPGPFGPRGPPGPVGPSGKDGSPGPLGPIGPPGVRGSVGEAGPEGPPGDPGPPGPPGPPGHLTAAIGDIMGHYDDAMADPLPEFTEDEAAPDDHNKTDPGVHATLKSLSSQIETMRSPDGSKKHPARTCDDLKLCHPSKKSGEYWIDPNQGCVEDAIKVYCNMETGETCISANPSTIPRKTWWASRSSDLKPIWYGLDMNRGSQFVYGDTAVTQMTFLRLLSKEASQNITYLCKNSVGYMDDQTKNLKKAVILKGANDLEIKAEGNSRFRYTVLQDSCSKRNGNVGKTVFEYRTQNVARLPIIDIAPVDVGSTDQEFGIEIGPVCFV

Target ORF information:

RefSeq Version	XM_004942396.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen type V alpha 2 chain (COL5A2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004942396.2

ORF Insert Sequence:

ATGATGGCAA TCAGGGTAGA AATGAGAACT TTTTTCATCC TTGCCGCTTT ATTAGGGCTA 
TTCGTCACCA TAAAAGCACA AGAAGAGGAA GATTACGATG CTGGAGAAGA TGTTGAAGAG 
ATTGCCTGCA CTCAGAATGG CCAGATGTAC CTGAACAGGG ATATTTGGAA GCCCTCACCG 
TGCCAGATTT GTGTCTGTGA CAACGGAGCT ATTCTTTGCG ATGAAATCCA GTGTCAAGAT 
GTGTTGGAGT GTGAGAACCC ACAGGTGCCC CCAGGAGAGT GCTGTCCAGT GTGTCCCCAT 
ACCGCACGTG ATTTTGACCC TACCGTTGGC AGAGGAAGAA AGGGGCAAAA AGGAGAACCT 
GGAATAGTGC CACCTGTTTC AGGAATACGA GGCCGCCCAG GGCCTGCAGG ACCTCCCGGT 
TCACAGGGAC CACGGGGAGA ACGAGGACCT AAGGGAAGAC CTGGTCCTCG TGGTCCTCAA 
GGGATTGATG GAGAACCCGG TATCCCTGGC CAGCCTGGTG ACCCTGGTCC TCCTGGGCAT 
CCAACTCACA CAGGACCAGA TGGGCTAAGC AGGCCATTTG CAGCTCAGAT GGCAGGTCTG 
GATGAAAAAT CAGGACTTGG TAGTCAGATG GGGCTGATGC CTGGTGCTGT GGGTCCAGTG 
GGTCCAAGAG GTCCTCAAGG TCTTCAAGGA AAACAAGGTG GTGCTGGCCC CACAGGCCCT 
CCTGGTGAAC CTGGAGATCC AGGACCTATG GGTCCAGTTG GTTCACGTGG ACCAGAAGGG 
CCTCCAGGCA AACCTGGTGA AGATGGTGAA CCCGGTAGAC CAGGACAGTC TGGTGAGCCT 
GGATTTCCAG GATCTCCAGG GCCTCGAGGC TTTCCTGGTG CTCCAGGTCT TCCAGGTCTC 
AAGGGTCACA GGGGATTGAA AGGTCTCGAT GGTCCAAAAG GTGAAATTGG AGCAGCTGGA 
GCAAAGGGAG AATCTGGACC AACAGGTGCC ATGGGTGCAA CAGGTCCCCT AGGACCTAGA 
GGAATGCCAG GAGAAAGAGG TCGAATTGGA CCACAGGGTG CCCCTGGTGG ACGTGGTCAG 
CATGGCATGC CTGGAAAGCC CGGGCCAATG GGTCCCCTTG GCATAACTGG TTCTCCAGGT 
TTTCCAGGCG CTCCTGGTAT GAAGGGAGAA GCAGGTCCAA CCGGTCCTCG TGGTCCTGAA 
GGCCCTCAAG GACAAAGAGG TGAAACTGGT CCACAAGGCC CAGCTGGATC ACAAGGTCTT 
CCTGGTACGG AAGGGACAGA TGGCTCTCCA GGTGCCAAAG GCCCAACTGG ATCTCCAGGC 
ACTGGGGGCC CCCCTGGCTT ATTGGGTCCA CCTGGTTCCC CAGGACCACA AGGCAGTACA 
GGCCCACCTG GTATTCGAGG GCAGCCGGGA GATCCTGGAG TTCCAGGTTT CAAGGGAGAA 
GCAGGACCCA AAGGTGAACC AGGCCCGCAA GGTCCCCAAG GTCCCATTGG CCCCATAGGT 
GAAGAAGGGA AAAGAGGCCC TCGAGGTGAT CCAGGGTCAG TGGGTCCACC AGGTCCAGTT 
GGAGAGAGGG GTGCTCCTGG GAACCGTGGT TTTCCAGGTT CTGATGGCTT GCCTGGACCA 
AAGGGTGCCC AAGGAGAGCG TGGTCCAGCA GGTTCTTCTG GTCCTAAAGG AGGTCAGGGA 
GATCCTGGGC GTCCTGGTGA GCCTGGACTC CCAGGTGCAA GGGGTTTGAC AGGAAATCCA 
GGTGTTCAAG GCCCTGAAGG AAAACTTGGC CCCTTGGGTG GCCCTGGAGA AGATGGACGT 
CCTGGTCCTG CAGGTTCGAT AGGAATCAGA GGTCAGCCAG GCAGTATGGG CCTTTCTGGG 
CCAAAGGGTA GTAGTGGTGA TCCTGGTAAA CCAGGAGAAG CAGGAAATGC AGGTGTTCCA 
GGACAAAGAG GAGCTCCTGG CAAAGACGGT GAAGTCGGTC CTTCTGGTCC TGTTGGCCCA 
CCAGGCCTGG CAGGAGAAAG AGGAGAACAA GGCCCTCCAG GTCCTACAGG GTTTCAGGGC 
TTACCTGGGC CTCCAGGTCC TCCTGGTGAA GGAGGAAAGC CAGGTGATCA GGGTGTTCCC 
GGAGATCCTG GAGTTGTTGG TCCATTGGGT CCCAGGGGAG AACGTGGCAA CCCGGGGGAA 
CGGGGAGAGC CAGGTGCATC AGGACTTGCA GGTGAAAAGG GTATGGCTGG AGGTCATGGT 
CCTGATGGGC CAAAGGGAAG CCCAGGCCCT AGTGGGACGC CTGGTGATCC AGGCCCACCA 
GGTCTTCAAG GTATGCCTGG AGAAAGAGGG ATTGCTGGAA CTCCTGGTCC AAAGGGTGAC 
AGAGGTGGTA TGGGTGAGAA AGGTGCTGAA GGTACTGCTG GCAATGATGG GGCAAGAGGT 
CTTCCTGGTC CAATAGGCCC AATGGGTCCA GCAGGGTCCA CTGGAGAAAA GGGTGAACCA 
GGTCCTCGAG GTCTTGTTGG TCCTCCCGGT TCTCGTGGAA ACCCTGGTTC TCGAGGAGAA 
AATGGCCCAA CTGGTGCTGT TGGTTTTGCT GGTCCTCCAG GCCCTGATGG TCAGCCGGGT 
GTGAAAGGTG AACCTGGGGA ACCTGGACAG AAAGGAGATG CTGGATCTCC AGGACCACAG 
GGTCTTGCTG GATCTCCTGG TCCACCAGGT CCTTCAGGTG TTCCTGGTCT GAAAGGTGGT 
CGAGGAACCC AGGGTCCCCC TGGTGCTACT GGATTCCCTG GATCTGCTGG AAGAGTTGGA 
CCTCCTGGAC CCACTGGAGC TCCAGGGCCT GCTGGGCCTA TTGGTGAGCC AGGAAAAGAA 
GGTCCCCCAG GTCTTCGTGG TGATCCTGGT TCTCATGGTC GTGTGGGTGA TCGAGGACCA 
GCTGGGCCCC CTGGTGGTCC TGGAGACAAG GGTGATGCAG GAGAAGATGG GCAACCGGGT 
CCAGATGGCC CCCCAGGTCC AGCAGGAACT ACTGGTCAAA GAGGTATTGT TGGTATGCCA 
GGACAGCGAG GGGAAAGAGG CATGCCAGGA CTGCCAGGTC CTGCAGGTAC ACCAGGAAAA 
CAAGGTCCCA CAGGGCCACC CGGTGATAAA GGTCCCCCAG GACCTATTGG CCAATCAGGT 
GCCACTGGAC CTGTAGGGGA AGCTGGTCCA GAAGGACCTG CTGGTAATGA TGGTACTCCA 
GGACGAGATG GAGCTGTTGG AGAGCGTGGT GATCGTGGTG AACCTGGCCC TGCAGGCCTA 
CCTGGTTCTC CAGGAGGTCC TGGAACTCCA GGTCCTGTTG GCCCCCCGGG AGATGCAGGT 
CAAAGAGGTG AAACTGGTTC TCGAGGGCCA GTGGGTCCCC CAGGCCGTGC AGGAAAAAGA 
GGTTTGCCTG GTCCCCAAGG GCCTCGTGGT GACAAAGGTG ACAATGGAGA TCGAGGTGAC 
AGGGGCCAGA AAGGACACAG AGGTTTCACT GGGCTTCAAG GTCTTCCTGG TCCTCCTGGC 
CCTATTGGTG AACAGGGCAG TCCTGGCATT CCAGGCCCAT TTGGTCCTCG GGGTCCTCCA 
GGTCCAGTTG GGCCTTCAGG CAAAGATGGA AGTCCTGGAC CACTAGGGCC GATTGGGCCT 
CCTGGTGTTA GAGGAAGTGT AGGAGAAGCT GGGCCTGAGG GTCCACCAGG TGATCCTGGC 
CCTCCTGGTC CCCCCGGCCC TCCAGGTCAC CTCACTGCTG CCATTGGTGA CATTATGGGA 
CACTATGATG ATGCTATGGC AGATCCACTA CCAGAGTTTA CTGAAGATGA AGCAGCTCCT 
GATGACCACA ATAAAACAGA CCCAGGAGTC CATGCCACGC TGAAGTCACT GAGCAGCCAG 
ATTGAGACTA TGCGCAGCCC AGATGGTTCG AAGAAACACC CAGCACGGAC TTGTGATGAC 
CTGAAGCTGT GCCACCCATC CAAGAAGAGT GGTGAATATT GGATTGATCC TAATCAGGGA 
TGTGTTGAAG ATGCTATAAA AGTATACTGC AACATGGAAA CTGGAGAGAC CTGCATTTCT 
GCAAATCCAT CAACTATCCC ACGTAAAACA TGGTGGGCCA GCAGATCATC TGACTTGAAG 
CCCATTTGGT ATGGCCTTGA TATGAACAGG GGATCACAGT TTGTGTATGG GGATACAGCT 
GTCACTCAAA TGACATTCCT GCGCCTCCTG TCAAAGGAAG CTTCCCAGAA TATCACTTAC 
CTCTGTAAGA ACAGTGTTGG GTACATGGAT GACCAAACTA AGAACCTCAA GAAAGCTGTC 
ATTCTGAAAG GGGCCAATGA CCTGGAGATC AAGGCGGAGG GAAACAGCCG ATTCAGATAC 
ACTGTTCTTC AAGACAGCTG TTCTAAACGT AATGGCAACG TGGGCAAGAC GGTCTTTGAG 
TACCGAACGC AGAACGTGGC ACGGTTGCCT ATCATAGATA TCGCACCAGT GGATGTTGGC 
AGCACTGACC AGGAATTTGG AATTGAAATT GGGCCAGTTT GTTTTGTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A2 ( XM_015289202.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015144688.1 Gallus gallus collagen type V alpha 2 chain (COL5A2), transcript variant X1, mRNA.

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CloneID	OGa38195
Clone ID Related Accession (Same CDS sequence)	XM_015289202.2 , XM_015289202.1
Accession Version	XM_015289202.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	collagen alpha-2(V) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289202.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCTGA GTGACTGCGA GGGGTGGAGC CAGAATCCAC CCCTTTCCAG ATCTCATTTA 
AGGGTTGGTA GTGAAGGCGA GGGCATCTTG CTGCAGATCC CCACCTACTG GAGGTCTTCC 
AAAGGAGAAG ATGTTGAAGA GATTGCCTGC ACTCAGAATG GCCAGATGTA CCTGAACAGG 
GATATTTGGA AGCCCTCACC GTGCCAGATT TGTGTCTGTG ACAACGGAGC TATTCTTTGC 
GATGAAATCC AGTGTCAAGA TGTGTTGGAG TGTGAGAACC CACAGGTGCC CCCAGGAGAG 
TGCTGTCCAG TGTGTCCCCA TACCGCACGT GATTTTGACC CTACCGTTGG CAGAGGAAGA 
AAGGGGCAAA AAGGAGAACC TGGAATAGTG CCACCTGTTT CAGGAATACG AGGCCGCCCA 
GGGCCTGCAG GACCTCCCGG TTCACAGGGA CCACGGGGAG AACGAGGACC TAAGGGAAGA 
CCTGGTCCTC GTGGTCCTCA AGGGATTGAT GGAGAACCCG GTATCCCTGG CCAGCCTGGT 
GACCCTGGTC CTCCTGGGCA TCCAACTCAC ACAGGACCAG ATGGGCTAAG CAGGCCATTT 
GCAGCTCAGA TGGCAGGTCT GGATGAAAAA TCAGGACTTG GTAGTCAGAT GGGGCTGATG 
CCTGGTGCTG TGGGTCCAGT GGGTCCAAGA GGTCCTCAAG GTCTTCAAGG AAAACAAGGT 
GGTGCTGGCC CCACAGGCCC TCCTGGTGAA CCTGGAGATC CAGGACCTAT GGGTCCAGTT 
GGTTCACGTG GACCAGAAGG GCCTCCAGGC AAACCTGGTG AAGATGGTGA ACCCGGTAGA 
CCAGGACAGT CTGGTGAGCC TGGATTTCCA GGATCTCCAG GGCCTCGAGG CTTTCCTGGT 
GCTCCAGGTC TTCCAGGTCT CAAGGGTCAC AGGGGATTGA AAGGTCTCGA TGGTCCAAAA 
GGTGAAATTG GAGCAGCTGG AGCAAAGGGA GAATCTGGAC CAACAGGTGC CATGGGTGCA 
ACAGGTCCCC TAGGACCTAG AGGAATGCCA GGAGAAAGAG GTCGAATTGG ACCACAGGGT 
GCCCCTGGTG GACGTGGTCA GCATGGCATG CCTGGAAAGC CCGGGCCAAT GGGTCCCCTT 
GGCATAACTG GTTCTCCAGG TTTTCCAGGC GCTCCTGGTA TGAAGGGAGA AGCAGGTCCA 
ACCGGTCCTC GTGGTCCTGA AGGCCCTCAA GGACAAAGAG GTGAAACTGG TCCACAAGGC 
CCAGCTGGAT CACAAGGTCT TCCTGGTACG GAAGGGACAG ATGGCTCTCC AGGTGCCAAA 
GGCCCAACTG GATCTCCAGG CACTGGGGGC CCCCCTGGCT TATTGGGTCC ACCTGGTTCC 
CCAGGACCAC AAGGCAGTAC AGGCCCACCT GGTATTCGAG GGCAGCCGGG AGATCCTGGA 
GTTCCAGGTT TCAAGGGAGA AGCAGGACCC AAAGGTGAAC CAGGCCCGCA AGGTCCCCAA 
GGTCCCATTG GCCCCATAGG TGAAGAAGGG AAAAGAGGCC CTCGAGGTGA TCCAGGGTCA 
GTGGGTCCAC CAGGTCCAGT TGGAGAGAGG GGTGCTCCTG GGAACCGTGG TTTTCCAGGT 
TCTGATGGCT TGCCTGGACC AAAGGGTGCC CAAGGAGAGC GTGGTCCAGC AGGTTCTTCT 
GGTCCTAAAG GAGGTCAGGG AGATCCTGGG CGTCCTGGTG AGCCTGGACT CCCAGGTGCA 
AGGGGTTTGA CAGGAAATCC AGGTGTTCAA GGCCCTGAAG GAAAACTTGG CCCCTTGGGT 
GGCCCTGGAG AAGATGGACG TCCTGGTCCT GCAGGTTCGA TAGGAATCAG AGGTCAGCCA 
GGCAGTATGG GCCTTTCTGG GCCAAAGGGT AGTAGTGGTG ATCCTGGTAA ACCAGGAGAA 
GCAGGAAATG CAGGTGTTCC AGGACAAAGA GGAGCTCCTG GCAAAGACGG TGAAGTCGGT 
CCTTCTGGTC CTGTTGGCCC ACCAGGCCTG GCAGGAGAAA GAGGAGAACA AGGCCCTCCA 
GGTCCTACAG GGTTTCAGGG CTTACCTGGG CCTCCAGGTC CTCCTGGTGA AGGAGGAAAG 
CCAGGTGATC AGGGTGTTCC CGGAGATCCT GGAGTTGTTG GTCCATTGGG TCCCAGGGGA 
GAACGTGGCA ACCCGGGGGA ACGGGGAGAG CCAGGTGCAT CAGGACTTGC AGGTGAAAAG 
GGTATGGCTG GAGGTCATGG TCCTGATGGG CCAAAGGGAA GCCCAGGCCC TAGTGGGACG 
CCTGGTGATC CAGGCCCACC AGGTCTTCAA GGTATGCCTG GAGAAAGAGG GATTGCTGGA 
ACTCCTGGTC CAAAGGGTGA CAGAGGTGGT ATGGGTGAGA AAGGTGCTGA AGGTACTGCT 
GGCAATGATG GGGCAAGAGG TCTTCCTGGT CCAATAGGCC CAATGGGTCC AGCAGGGTCC 
ACTGGAGAAA AGGGTGAACC AGGTCCTCGA GGTCTTGTTG GTCCTCCCGG TTCTCGTGGA 
AACCCTGGTT CTCGAGGAGA AAATGGCCCA ACTGGTGCTG TTGGTTTTGC TGGTCCTCCA 
GGCCCTGATG GTCAGCCGGG TGTGAAAGGT GAACCTGGGG AACCTGGACA GAAAGGAGAT 
GCTGGATCTC CAGGACCACA GGGTCTTGCT GGATCTCCTG GTCCACCAGG TCCTTCAGGT 
GTTCCTGGTC TGAAAGGTGG TCGAGGAACC CAGGGTCCCC CTGGTGCTAC TGGATTCCCT 
GGATCTGCTG GAAGAGTTGG ACCTCCTGGA CCCACTGGAG CTCCAGGGCC TGCTGGGCCT 
ATTGGTGAGC CAGGAAAAGA AGGTCCCCCA GGTCTTCGTG GTGATCCTGG TTCTCATGGT 
CGTGTGGGTG ATCGAGGACC AGCTGGGCCC CCTGGTGGTC CTGGAGACAA GGGTGATGCA 
GGAGAAGATG GGCAACCGGG TCCAGATGGC CCCCCAGGTC CAGCAGGAAC TACTGGTCAA 
AGAGGTATTG TTGGTATGCC AGGACAGCGA GGGGAAAGAG GCATGCCAGG ACTGCCAGGT 
CCTGCAGGTA CACCAGGAAA ACAAGGTCCC ACAGGGCCAC CCGGTGATAA AGGTCCCCCA 
GGACCTATTG GCCAATCAGG TGCCACTGGA CCTGTAGGGG AAGCTGGTCC AGAAGGACCT 
GCTGGTAATG ATGGTACTCC AGGACGAGAT GGAGCTGTTG GAGAGCGTGG TGATCGTGGT 
GAACCTGGCC CTGCAGGCCT ACCTGGTTCT CCAGGAGGTC CTGGAACTCC AGGTCCTGTT 
GGCCCCCCGG GAGATGCAGG TCAAAGAGGT GAAACTGGTT CTCGAGGGCC AGTGGGTCCC 
CCAGGCCGTG CAGGAAAAAG AGGTTTGCCT GGTCCCCAAG GGCCTCGTGG TGACAAAGGT 
GACAATGGAG ATCGAGGTGA CAGGGGCCAG AAAGGACACA GAGGTTTCAC TGGGCTTCAA 
GGTCTTCCTG GTCCTCCTGG CCCTATTGGT GAACAGGGCA GTCCTGGCAT TCCAGGCCCA 
TTTGGTCCTC GGGGTCCTCC AGGTCCAGTT GGGCCTTCAG GCAAAGATGG AAGTCCTGGA 
CCACTAGGGC CGATTGGGCC TCCTGGTGTT AGAGGAAGTG TAGGAGAAGC TGGGCCTGAG 
GGTCCACCAG GTGATCCTGG CCCTCCTGGT CCCCCCGGCC CTCCAGGTCA CCTCACTGCT 
GCCATTGGTG ACATTATGGG ACACTATGAT GATGCTATGG CAGATCCACT ACCAGAGTTT 
ACTGAAGATG AAGCAGCTCC TGATGACCAC AATAAAACAG ACCCAGGAGT CCATGCCACG 
CTGAAGTCAC TGAGCAGCCA GATTGAGACT ATGCGCAGCC CAGATGGTTC GAAGAAACAC 
CCAGCACGGA CTTGTGATGA CCTGAAGCTG TGCCACCCAT CCAAGAAGAG TGGTGAATAT 
TGGATTGATC CTAATCAGGG ATGTGTTGAA GATGCTATAA AAGTATACTG CAACATGGAA 
ACTGGAGAGA CCTGCATTTC TGCAAATCCA TCAACTATCC CACGTAAAAC ATGGTGGGCC 
AGCAGATCAT CTGACTTGAA GCCCATTTGG TATGGCCTTG ATATGAACAG GGGATCACAG 
TTTGTGTATG GGGATACAGC TGTCACTCAA ATGACATTCC TGCGCCTCCT GTCAAAGGAA 
GCTTCCCAGA ATATCACTTA CCTCTGTAAG AACAGTGTTG GGTACATGGA TGACCAAACT 
AAGAACCTCA AGAAAGCTGT CATTCTGAAA GGGGCCAATG ACCTGGAGAT CAAGGCGGAG 
GGAAACAGCC GATTCAGATA CACTGTTCTT CAAGACAGCT GTTCTAAACG TAATGGCAAC 
GTGGGCAAGA CGGTCTTTGA GTACCGAACG CAGAACGTGG CACGGTTGCC TATCATAGAT 
ATCGCACCAG TGGATGTTGG CAGCACTGAC CAGGAATTTG GAATTGAAAT TGGGCCAGTT 
TGTTTTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015144688.1
CDS	16..4527
Translation	MHLSDCEGWSQNPPLSRSHLRVGSEGEGILLQIPTYWRSSKGEDVEEIACTQNGQMYLNRDIWKPSPCQICVCDNGAILCDEIQCQDVLECENPQVPPGECCPVCPHTARDFDPTVGRGRKGQKGEPGIVPPVSGIRGRPGPAGPPGSQGPRGERGPKGRPGPRGPQGIDGEPGIPGQPGDPGPPGHPTHTGPDGLSRPFAAQMAGLDEKSGLGSQMGLMPGAVGPVGPRGPQGLQGKQGGAGPTGPPGEPGDPGPMGPVGSRGPEGPPGKPGEDGEPGRPGQSGEPGFPGSPGPRGFPGAPGLPGLKGHRGLKGLDGPKGEIGAAGAKGESGPTGAMGATGPLGPRGMPGERGRIGPQGAPGGRGQHGMPGKPGPMGPLGITGSPGFPGAPGMKGEAGPTGPRGPEGPQGQRGETGPQGPAGSQGLPGTEGTDGSPGAKGPTGSPGTGGPPGLLGPPGSPGPQGSTGPPGIRGQPGDPGVPGFKGEAGPKGEPGPQGPQGPIGPIGEEGKRGPRGDPGSVGPPGPVGERGAPGNRGFPGSDGLPGPKGAQGERGPAGSSGPKGGQGDPGRPGEPGLPGARGLTGNPGVQGPEGKLGPLGGPGEDGRPGPAGSIGIRGQPGSMGLSGPKGSSGDPGKPGEAGNAGVPGQRGAPGKDGEVGPSGPVGPPGLAGERGEQGPPGPTGFQGLPGPPGPPGEGGKPGDQGVPGDPGVVGPLGPRGERGNPGERGEPGASGLAGEKGMAGGHGPDGPKGSPGPSGTPGDPGPPGLQGMPGERGIAGTPGPKGDRGGMGEKGAEGTAGNDGARGLPGPIGPMGPAGSTGEKGEPGPRGLVGPPGSRGNPGSRGENGPTGAVGFAGPPGPDGQPGVKGEPGEPGQKGDAGSPGPQGLAGSPGPPGPSGVPGLKGGRGTQGPPGATGFPGSAGRVGPPGPTGAPGPAGPIGEPGKEGPPGLRGDPGSHGRVGDRGPAGPPGGPGDKGDAGEDGQPGPDGPPGPAGTTGQRGIVGMPGQRGERGMPGLPGPAGTPGKQGPTGPPGDKGPPGPIGQSGATGPVGEAGPEGPAGNDGTPGRDGAVGERGDRGEPGPAGLPGSPGGPGTPGPVGPPGDAGQRGETGSRGPVGPPGRAGKRGLPGPQGPRGDKGDNGDRGDRGQKGHRGFTGLQGLPGPPGPIGEQGSPGIPGPFGPRGPPGPVGPSGKDGSPGPLGPIGPPGVRGSVGEAGPEGPPGDPGPPGPPGPPGHLTAAIGDIMGHYDDAMADPLPEFTEDEAAPDDHNKTDPGVHATLKSLSSQIETMRSPDGSKKHPARTCDDLKLCHPSKKSGEYWIDPNQGCVEDAIKVYCNMETGETCISANPSTIPRKTWWASRSSDLKPIWYGLDMNRGSQFVYGDTAVTQMTFLRLLSKEASQNITYLCKNSVGYMDDQTKNLKKAVILKGANDLEIKAEGNSRFRYTVLQDSCSKRNGNVGKTVFEYRTQNVARLPIIDIAPVDVGSTDQEFGIEIGPVCFV

Target ORF information:

RefSeq Version	XM_015289202.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen type V alpha 2 chain (COL5A2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289202.2

ORF Insert Sequence:

ATGCATCTGA GTGACTGCGA GGGGTGGAGC CAGAATCCAC CCCTTTCCAG ATCTCATTTA 
AGGGTTGGTA GTGAAGGCGA GGGCATCTTG CTGCAGATCC CCACCTACTG GAGGTCTTCC 
AAAGGAGAAG ATGTTGAAGA GATTGCCTGC ACTCAGAATG GCCAGATGTA CCTGAACAGG 
GATATTTGGA AGCCCTCACC GTGCCAGATT TGTGTCTGTG ACAACGGAGC TATTCTTTGC 
GATGAAATCC AGTGTCAAGA TGTGTTGGAG TGTGAGAACC CACAGGTGCC CCCAGGAGAG 
TGCTGTCCAG TGTGTCCCCA TACCGCACGT GATTTTGACC CTACCGTTGG CAGAGGAAGA 
AAGGGGCAAA AAGGAGAACC TGGAATAGTG CCACCTGTTT CAGGAATACG AGGCCGCCCA 
GGGCCTGCAG GACCTCCCGG TTCACAGGGA CCACGGGGAG AACGAGGACC TAAGGGAAGA 
CCTGGTCCTC GTGGTCCTCA AGGGATTGAT GGAGAACCCG GTATCCCTGG CCAGCCTGGT 
GACCCTGGTC CTCCTGGGCA TCCAACTCAC ACAGGACCAG ATGGGCTAAG CAGGCCATTT 
GCAGCTCAGA TGGCAGGTCT GGATGAAAAA TCAGGACTTG GTAGTCAGAT GGGGCTGATG 
CCTGGTGCTG TGGGTCCAGT GGGTCCAAGA GGTCCTCAAG GTCTTCAAGG AAAACAAGGT 
GGTGCTGGCC CCACAGGCCC TCCTGGTGAA CCTGGAGATC CAGGACCTAT GGGTCCAGTT 
GGTTCACGTG GACCAGAAGG GCCTCCAGGC AAACCTGGTG AAGATGGTGA ACCCGGTAGA 
CCAGGACAGT CTGGTGAGCC TGGATTTCCA GGATCTCCAG GGCCTCGAGG CTTTCCTGGT 
GCTCCAGGTC TTCCAGGTCT CAAGGGTCAC AGGGGATTGA AAGGTCTCGA TGGTCCAAAA 
GGTGAAATTG GAGCAGCTGG AGCAAAGGGA GAATCTGGAC CAACAGGTGC CATGGGTGCA 
ACAGGTCCCC TAGGACCTAG AGGAATGCCA GGAGAAAGAG GTCGAATTGG ACCACAGGGT 
GCCCCTGGTG GACGTGGTCA GCATGGCATG CCTGGAAAGC CCGGGCCAAT GGGTCCCCTT 
GGCATAACTG GTTCTCCAGG TTTTCCAGGC GCTCCTGGTA TGAAGGGAGA AGCAGGTCCA 
ACCGGTCCTC GTGGTCCTGA AGGCCCTCAA GGACAAAGAG GTGAAACTGG TCCACAAGGC 
CCAGCTGGAT CACAAGGTCT TCCTGGTACG GAAGGGACAG ATGGCTCTCC AGGTGCCAAA 
GGCCCAACTG GATCTCCAGG CACTGGGGGC CCCCCTGGCT TATTGGGTCC ACCTGGTTCC 
CCAGGACCAC AAGGCAGTAC AGGCCCACCT GGTATTCGAG GGCAGCCGGG AGATCCTGGA 
GTTCCAGGTT TCAAGGGAGA AGCAGGACCC AAAGGTGAAC CAGGCCCGCA AGGTCCCCAA 
GGTCCCATTG GCCCCATAGG TGAAGAAGGG AAAAGAGGCC CTCGAGGTGA TCCAGGGTCA 
GTGGGTCCAC CAGGTCCAGT TGGAGAGAGG GGTGCTCCTG GGAACCGTGG TTTTCCAGGT 
TCTGATGGCT TGCCTGGACC AAAGGGTGCC CAAGGAGAGC GTGGTCCAGC AGGTTCTTCT 
GGTCCTAAAG GAGGTCAGGG AGATCCTGGG CGTCCTGGTG AGCCTGGACT CCCAGGTGCA 
AGGGGTTTGA CAGGAAATCC AGGTGTTCAA GGCCCTGAAG GAAAACTTGG CCCCTTGGGT 
GGCCCTGGAG AAGATGGACG TCCTGGTCCT GCAGGTTCGA TAGGAATCAG AGGTCAGCCA 
GGCAGTATGG GCCTTTCTGG GCCAAAGGGT AGTAGTGGTG ATCCTGGTAA ACCAGGAGAA 
GCAGGAAATG CAGGTGTTCC AGGACAAAGA GGAGCTCCTG GCAAAGACGG TGAAGTCGGT 
CCTTCTGGTC CTGTTGGCCC ACCAGGCCTG GCAGGAGAAA GAGGAGAACA AGGCCCTCCA 
GGTCCTACAG GGTTTCAGGG CTTACCTGGG CCTCCAGGTC CTCCTGGTGA AGGAGGAAAG 
CCAGGTGATC AGGGTGTTCC CGGAGATCCT GGAGTTGTTG GTCCATTGGG TCCCAGGGGA 
GAACGTGGCA ACCCGGGGGA ACGGGGAGAG CCAGGTGCAT CAGGACTTGC AGGTGAAAAG 
GGTATGGCTG GAGGTCATGG TCCTGATGGG CCAAAGGGAA GCCCAGGCCC TAGTGGGACG 
CCTGGTGATC CAGGCCCACC AGGTCTTCAA GGTATGCCTG GAGAAAGAGG GATTGCTGGA 
ACTCCTGGTC CAAAGGGTGA CAGAGGTGGT ATGGGTGAGA AAGGTGCTGA AGGTACTGCT 
GGCAATGATG GGGCAAGAGG TCTTCCTGGT CCAATAGGCC CAATGGGTCC AGCAGGGTCC 
ACTGGAGAAA AGGGTGAACC AGGTCCTCGA GGTCTTGTTG GTCCTCCCGG TTCTCGTGGA 
AACCCTGGTT CTCGAGGAGA AAATGGCCCA ACTGGTGCTG TTGGTTTTGC TGGTCCTCCA 
GGCCCTGATG GTCAGCCGGG TGTGAAAGGT GAACCTGGGG AACCTGGACA GAAAGGAGAT 
GCTGGATCTC CAGGACCACA GGGTCTTGCT GGATCTCCTG GTCCACCAGG TCCTTCAGGT 
GTTCCTGGTC TGAAAGGTGG TCGAGGAACC CAGGGTCCCC CTGGTGCTAC TGGATTCCCT 
GGATCTGCTG GAAGAGTTGG ACCTCCTGGA CCCACTGGAG CTCCAGGGCC TGCTGGGCCT 
ATTGGTGAGC CAGGAAAAGA AGGTCCCCCA GGTCTTCGTG GTGATCCTGG TTCTCATGGT 
CGTGTGGGTG ATCGAGGACC AGCTGGGCCC CCTGGTGGTC CTGGAGACAA GGGTGATGCA 
GGAGAAGATG GGCAACCGGG TCCAGATGGC CCCCCAGGTC CAGCAGGAAC TACTGGTCAA 
AGAGGTATTG TTGGTATGCC AGGACAGCGA GGGGAAAGAG GCATGCCAGG ACTGCCAGGT 
CCTGCAGGTA CACCAGGAAA ACAAGGTCCC ACAGGGCCAC CCGGTGATAA AGGTCCCCCA 
GGACCTATTG GCCAATCAGG TGCCACTGGA CCTGTAGGGG AAGCTGGTCC AGAAGGACCT 
GCTGGTAATG ATGGTACTCC AGGACGAGAT GGAGCTGTTG GAGAGCGTGG TGATCGTGGT 
GAACCTGGCC CTGCAGGCCT ACCTGGTTCT CCAGGAGGTC CTGGAACTCC AGGTCCTGTT 
GGCCCCCCGG GAGATGCAGG TCAAAGAGGT GAAACTGGTT CTCGAGGGCC AGTGGGTCCC 
CCAGGCCGTG CAGGAAAAAG AGGTTTGCCT GGTCCCCAAG GGCCTCGTGG TGACAAAGGT 
GACAATGGAG ATCGAGGTGA CAGGGGCCAG AAAGGACACA GAGGTTTCAC TGGGCTTCAA 
GGTCTTCCTG GTCCTCCTGG CCCTATTGGT GAACAGGGCA GTCCTGGCAT TCCAGGCCCA 
TTTGGTCCTC GGGGTCCTCC AGGTCCAGTT GGGCCTTCAG GCAAAGATGG AAGTCCTGGA 
CCACTAGGGC CGATTGGGCC TCCTGGTGTT AGAGGAAGTG TAGGAGAAGC TGGGCCTGAG 
GGTCCACCAG GTGATCCTGG CCCTCCTGGT CCCCCCGGCC CTCCAGGTCA CCTCACTGCT 
GCCATTGGTG ACATTATGGG ACACTATGAT GATGCTATGG CAGATCCACT ACCAGAGTTT 
ACTGAAGATG AAGCAGCTCC TGATGACCAC AATAAAACAG ACCCAGGAGT CCATGCCACG 
CTGAAGTCAC TGAGCAGCCA GATTGAGACT ATGCGCAGCC CAGATGGTTC GAAGAAACAC 
CCAGCACGGA CTTGTGATGA CCTGAAGCTG TGCCACCCAT CCAAGAAGAG TGGTGAATAT 
TGGATTGATC CTAATCAGGG ATGTGTTGAA GATGCTATAA AAGTATACTG CAACATGGAA 
ACTGGAGAGA CCTGCATTTC TGCAAATCCA TCAACTATCC CACGTAAAAC ATGGTGGGCC 
AGCAGATCAT CTGACTTGAA GCCCATTTGG TATGGCCTTG ATATGAACAG GGGATCACAG 
TTTGTGTATG GGGATACAGC TGTCACTCAA ATGACATTCC TGCGCCTCCT GTCAAAGGAA 
GCTTCCCAGA ATATCACTTA CCTCTGTAAG AACAGTGTTG GGTACATGGA TGACCAAACT 
AAGAACCTCA AGAAAGCTGT CATTCTGAAA GGGGCCAATG ACCTGGAGAT CAAGGCGGAG 
GGAAACAGCC GATTCAGATA CACTGTTCTT CAAGACAGCT GTTCTAAACG TAATGGCAAC 
GTGGGCAAGA CGGTCTTTGA GTACCGAACG CAGAACGTGG CACGGTTGCC TATCATAGAT 
ATCGCACCAG TGGATGTTGG CAGCACTGAC CAGGAATTTG GAATTGAAAT TGGGCCAGTT 
TGTTTTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A2 ( XM_015289202.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015144688.1 Gallus gallus collagen, type V, alpha 2 (COL5A2), transcript variant X1, mRNA.

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CloneID	OGa38195
Clone ID Related Accession (Same CDS sequence)	XM_015289202.2 , XM_015289202.1
Accession Version	XM_015289202.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	collagen alpha-2(V) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455901.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCTGA GTGACTGCGA GGGGTGGAGC CAGAATCCAC CCCTTTCCAG ATCTCATTTA 
AGGGTTGGTA GTGAAGGCGA GGGCATCTTG CTGCAGATCC CCACCTACTG GAGGTCTTCC 
AAAGGAGAAG ATGTTGAAGA GATTGCCTGC ACTCAGAATG GCCAGATGTA CCTGAACAGG 
GATATTTGGA AGCCCTCACC GTGCCAGATT TGTGTCTGTG ACAACGGAGC TATTCTTTGC 
GATGAAATCC AGTGTCAAGA TGTGTTGGAG TGTGAGAACC CACAGGTGCC CCCAGGAGAG 
TGCTGTCCAG TGTGTCCCCA TACCGCACGT GATTTTGACC CTACCGTTGG CAGAGGAAGA 
AAGGGGCAAA AAGGAGAACC TGGAATAGTG CCACCTGTTT CAGGAATACG AGGCCGCCCA 
GGGCCTGCAG GACCTCCCGG TTCACAGGGA CCACGGGGAG AACGAGGACC TAAGGGAAGA 
CCTGGTCCTC GTGGTCCTCA AGGGATTGAT GGAGAACCCG GTATCCCTGG CCAGCCTGGT 
GACCCTGGTC CTCCTGGGCA TCCAACTCAC ACAGGACCAG ATGGGCTAAG CAGGCCATTT 
GCAGCTCAGA TGGCAGGTCT GGATGAAAAA TCAGGACTTG GTAGTCAGAT GGGGCTGATG 
CCTGGTGCTG TGGGTCCAGT GGGTCCAAGA GGTCCTCAAG GTCTTCAAGG AAAACAAGGT 
GGTGCTGGCC CCACAGGCCC TCCTGGTGAA CCTGGAGATC CAGGACCTAT GGGTCCAGTT 
GGTTCACGTG GACCAGAAGG GCCTCCAGGC AAACCTGGTG AAGATGGTGA ACCCGGTAGA 
CCAGGACAGT CTGGTGAGCC TGGATTTCCA GGATCTCCAG GGCCTCGAGG CTTTCCTGGT 
GCTCCAGGTC TTCCAGGTCT CAAGGGTCAC AGGGGATTGA AAGGTCTCGA TGGTCCAAAA 
GGTGAAATTG GAGCAGCTGG AGCAAAGGGA GAATCTGGAC CAACAGGTGC CATGGGTGCA 
ACAGGTCCCC TAGGACCTAG AGGAATGCCA GGAGAAAGAG GTCGAATTGG ACCACAGGGT 
GCCCCTGGTG GACGTGGTCA GCATGGCATG CCTGGAAAGC CCGGGCCAAT GGGTCCCCTT 
GGCATAACTG GTTCTCCAGG TTTTCCAGGC GCTCCTGGTA TGAAGGGAGA AGCAGGTCCA 
ACCGGTCCTC GTGGTCCTGA AGGCCCTCAA GGACAAAGAG GTGAAACTGG TCCACAAGGC 
CCAGCTGGAT CACAAGGTCT TCCTGGTACG GAAGGGACAG ATGGCTCTCC AGGTGCCAAA 
GGCCCAACTG GATCTCCAGG CACTGGGGGC CCCCCTGGCT TATTGGGTCC ACCTGGTTCC 
CCAGGACCAC AAGGCAGTAC AGGCCCACCT GGTATTCGAG GGCAGCCGGG AGATCCTGGA 
GTTCCAGGTT TCAAGGGAGA AGCAGGACCC AAAGGTGAAC CAGGCCCGCA AGGTCCCCAA 
GGTCCCATTG GCCCCATAGG TGAAGAAGGG AAAAGAGGCC CTCGAGGTGA TCCAGGGTCA 
GTGGGTCCAC CAGGTCCAGT TGGAGAGAGG GGTGCTCCTG GGAACCGTGG TTTTCCAGGT 
TCTGATGGCT TGCCTGGACC AAAGGGTGCC CAAGGAGAGC GTGGTCCAGC AGGTTCTTCT 
GGTCCTAAAG GAGGTCAGGG AGATCCTGGG CGTCCTGGTG AGCCTGGACT CCCAGGTGCA 
AGGGGTTTGA CAGGAAATCC AGGTGTTCAA GGCCCTGAAG GAAAACTTGG CCCCTTGGGT 
GGCCCTGGAG AAGATGGACG TCCTGGTCCT GCAGGTTCGA TAGGAATCAG AGGTCAGCCA 
GGCAGTATGG GCCTTTCTGG GCCAAAGGGT AGTAGTGGTG ATCCTGGTAA ACCAGGAGAA 
GCAGGAAATG CAGGTGTTCC AGGACAAAGA GGAGCTCCTG GCAAAGACGG TGAAGTCGGT 
CCTTCTGGTC CTGTTGGCCC ACCAGGCCTG GCAGGAGAAA GAGGAGAACA AGGCCCTCCA 
GGTCCTACAG GGTTTCAGGG CTTACCTGGG CCTCCAGGTC CTCCTGGTGA AGGAGGAAAG 
CCAGGTGATC AGGGTGTTCC CGGAGATCCT GGAGTTGTTG GTCCATTGGG TCCCAGGGGA 
GAACGTGGCA ACCCGGGGGA ACGGGGAGAG CCAGGTGCAT CAGGACTTGC AGGTGAAAAG 
GGTATGGCTG GAGGTCATGG TCCTGATGGG CCAAAGGGAA GCCCAGGCCC TAGTGGGACG 
CCTGGTGATC CAGGCCCACC AGGTCTTCAA GGTATGCCTG GAGAAAGAGG GATTGCTGGA 
ACTCCTGGTC CAAAGGGTGA CAGAGGTGGT ATGGGTGAGA AAGGTGCTGA AGGTACTGCT 
GGCAATGATG GGGCAAGAGG TCTTCCTGGT CCAATAGGCC CAATGGGTCC AGCAGGGTCC 
ACTGGAGAAA AGGGTGAACC AGGTCCTCGA GGTCTTGTTG GTCCTCCCGG TTCTCGTGGA 
AACCCTGGTT CTCGAGGAGA AAATGGCCCA ACTGGTGCTG TTGGTTTTGC TGGTCCTCCA 
GGCCCTGATG GTCAGCCGGG TGTGAAAGGT GAACCTGGGG AACCTGGACA GAAAGGAGAT 
GCTGGATCTC CAGGACCACA GGGTCTTGCT GGATCTCCTG GTCCACCAGG TCCTTCAGGT 
GTTCCTGGTC TGAAAGGTGG TCGAGGAACC CAGGGTCCCC CTGGTGCTAC TGGATTCCCT 
GGATCTGCTG GAAGAGTTGG ACCTCCTGGA CCCACTGGAG CTCCAGGGCC TGCTGGGCCT 
ATTGGTGAGC CAGGAAAAGA AGGTCCCCCA GGTCTTCGTG GTGATCCTGG TTCTCATGGT 
CGTGTGGGTG ATCGAGGACC AGCTGGGCCC CCTGGTGGTC CTGGAGACAA GGGTGATGCA 
GGAGAAGATG GGCAACCGGG TCCAGATGGC CCCCCAGGTC CAGCAGGAAC TACTGGTCAA 
AGAGGTATTG TTGGTATGCC AGGACAGCGA GGGGAAAGAG GCATGCCAGG ACTGCCAGGT 
CCTGCAGGTA CACCAGGAAA ACAAGGTCCC ACAGGGCCAC CCGGTGATAA AGGTCCCCCA 
GGACCTATTG GCCAATCAGG TGCCACTGGA CCTGTAGGGG AAGCTGGTCC AGAAGGACCT 
GCTGGTAATG ATGGTACTCC AGGACGAGAT GGAGCTGTTG GAGAGCGTGG TGATCGTGGT 
GAACCTGGCC CTGCAGGCCT ACCTGGTTCT CCAGGAGGTC CTGGAACTCC AGGTCCTGTT 
GGCCCCCCGG GAGATGCAGG TCAAAGAGGT GAAACTGGTT CTCGAGGGCC AGTGGGTCCC 
CCAGGCCGTG CAGGAAAAAG AGGTTTGCCT GGTCCCCAAG GGCCTCGTGG TGACAAAGGT 
GACAATGGAG ATCGAGGTGA CAGGGGCCAG AAAGGACACA GAGGTTTCAC TGGGCTTCAA 
GGTCTTCCTG GTCCTCCTGG CCCTATTGGT GAACAGGGCA GTCCTGGCAT TCCAGGCCCA 
TTTGGTCCTC GGGGTCCTCC AGGTCCAGTT GGGCCTTCAG GCAAAGATGG AAGTCCTGGA 
CCACTAGGGC CGATTGGGCC TCCTGGTGTT AGAGGAAGTG TAGGAGAAGC TGGGCCTGAG 
GGTCCACCAG GTGATCCTGG CCCTCCTGGT CCCCCCGGCC CTCCAGGTCA CCTCACTGCT 
GCCATTGGTG ACATTATGGG ACACTATGAT GATGCTATGG CAGATCCACT ACCAGAGTTT 
ACTGAAGATG AAGCAGCTCC TGATGACCAC AATAAAACAG ACCCAGGAGT CCATGCCACG 
CTGAAGTCAC TGAGCAGCCA GATTGAGACT ATGCGCAGCC CAGATGGTTC GAAGAAACAC 
CCAGCACGGA CTTGTGATGA CCTGAAGCTG TGCCACCCAT CCAAGAAGAG TGGTGAATAT 
TGGATTGATC CTAATCAGGG ATGTGTTGAA GATGCTATAA AAGTATACTG CAACATGGAA 
ACTGGAGAGA CCTGCATTTC TGCAAATCCA TCAACTATCC CACGTAAAAC ATGGTGGGCC 
AGCAGATCAT CTGACTTGAA GCCCATTTGG TATGGCCTTG ATATGAACAG GGGATCACAG 
TTTGTGTATG GGGATACAGC TGTCACTCAA ATGACATTCC TGCGCCTCCT GTCAAAGGAA 
GCTTCCCAGA ATATCACTTA CCTCTGTAAG AACAGTGTTG GGTACATGGA TGACCAAACT 
AAGAACCTCA AGAAAGCTGT CATTCTGAAA GGGGCCAATG ACCTGGAGAT CAAGGCGGAG 
GGAAACAGCC GATTCAGATA CACTGTTCTT CAAGACAGCT GTTCTAAACG TAATGGCAAC 
GTGGGCAAGA CGGTCTTTGA GTACCGAACG CAGAACGTGG CACGGTTGCC TATCATAGAT 
ATCGCACCAG TGGATGTTGG CAGCACTGAC CAGGAATTTG GAATTGAAAT TGGGCCAGTT 
TGTTTTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015144688.1
CDS	20..4531
Translation	MHLSDCEGWSQNPPLSRSHLRVGSEGEGILLQIPTYWRSSKGEDVEEIACTQNGQMYLNRDIWKPSPCQICVCDNGAILCDEIQCQDVLECENPQVPPGECCPVCPHTARDFDPTVGRGRKGQKGEPGIVPPVSGIRGRPGPAGPPGSQGPRGERGPKGRPGPRGPQGIDGEPGIPGQPGDPGPPGHPTHTGPDGLSRPFAAQMAGLDEKSGLGSQMGLMPGAVGPVGPRGPQGLQGKQGGAGPTGPPGEPGDPGPMGPVGSRGPEGPPGKPGEDGEPGRPGQSGEPGFPGSPGPRGFPGAPGLPGLKGHRGLKGLDGPKGEIGAAGAKGESGPTGAMGATGPLGPRGMPGERGRIGPQGAPGGRGQHGMPGKPGPMGPLGITGSPGFPGAPGMKGEAGPTGPRGPEGPQGQRGETGPQGPAGSQGLPGTEGTDGSPGAKGPTGSPGTGGPPGLLGPPGSPGPQGSTGPPGIRGQPGDPGVPGFKGEAGPKGEPGPQGPQGPIGPIGEEGKRGPRGDPGSVGPPGPVGERGAPGNRGFPGSDGLPGPKGAQGERGPAGSSGPKGGQGDPGRPGEPGLPGARGLTGNPGVQGPEGKLGPLGGPGEDGRPGPAGSIGIRGQPGSMGLSGPKGSSGDPGKPGEAGNAGVPGQRGAPGKDGEVGPSGPVGPPGLAGERGEQGPPGPTGFQGLPGPPGPPGEGGKPGDQGVPGDPGVVGPLGPRGERGNPGERGEPGASGLAGEKGMAGGHGPDGPKGSPGPSGTPGDPGPPGLQGMPGERGIAGTPGPKGDRGGMGEKGAEGTAGNDGARGLPGPIGPMGPAGSTGEKGEPGPRGLVGPPGSRGNPGSRGENGPTGAVGFAGPPGPDGQPGVKGEPGEPGQKGDAGSPGPQGLAGSPGPPGPSGVPGLKGGRGTQGPPGATGFPGSAGRVGPPGPTGAPGPAGPIGEPGKEGPPGLRGDPGSHGRVGDRGPAGPPGGPGDKGDAGEDGQPGPDGPPGPAGTTGQRGIVGMPGQRGERGMPGLPGPAGTPGKQGPTGPPGDKGPPGPIGQSGATGPVGEAGPEGPAGNDGTPGRDGAVGERGDRGEPGPAGLPGSPGGPGTPGPVGPPGDAGQRGETGSRGPVGPPGRAGKRGLPGPQGPRGDKGDNGDRGDRGQKGHRGFTGLQGLPGPPGPIGEQGSPGIPGPFGPRGPPGPVGPSGKDGSPGPLGPIGPPGVRGSVGEAGPEGPPGDPGPPGPPGPPGHLTAAIGDIMGHYDDAMADPLPEFTEDEAAPDDHNKTDPGVHATLKSLSSQIETMRSPDGSKKHPARTCDDLKLCHPSKKSGEYWIDPNQGCVEDAIKVYCNMETGETCISANPSTIPRKTWWASRSSDLKPIWYGLDMNRGSQFVYGDTAVTQMTFLRLLSKEASQNITYLCKNSVGYMDDQTKNLKKAVILKGANDLEIKAEGNSRFRYTVLQDSCSKRNGNVGKTVFEYRTQNVARLPIIDIAPVDVGSTDQEFGIEIGPVCFV

Target ORF information:

RefSeq Version	XM_015289202.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen, type V, alpha 2 (COL5A2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289202.1

ORF Insert Sequence:

ATGCATCTGA GTGACTGCGA GGGGTGGAGC CAGAATCCAC CCCTTTCCAG ATCTCATTTA 
AGGGTTGGTA GTGAAGGCGA GGGCATCTTG CTGCAGATCC CCACCTACTG GAGGTCTTCC 
AAAGGAGAAG ATGTTGAAGA GATTGCCTGC ACTCAGAATG GCCAGATGTA CCTGAACAGG 
GATATTTGGA AGCCCTCACC GTGCCAGATT TGTGTCTGTG ACAACGGAGC TATTCTTTGC 
GATGAAATCC AGTGTCAAGA TGTGTTGGAG TGTGAGAACC CACAGGTGCC CCCAGGAGAG 
TGCTGTCCAG TGTGTCCCCA TACCGCACGT GATTTTGACC CTACCGTTGG CAGAGGAAGA 
AAGGGGCAAA AAGGAGAACC TGGAATAGTG CCACCTGTTT CAGGAATACG AGGCCGCCCA 
GGGCCTGCAG GACCTCCCGG TTCACAGGGA CCACGGGGAG AACGAGGACC TAAGGGAAGA 
CCTGGTCCTC GTGGTCCTCA AGGGATTGAT GGAGAACCCG GTATCCCTGG CCAGCCTGGT 
GACCCTGGTC CTCCTGGGCA TCCAACTCAC ACAGGACCAG ATGGGCTAAG CAGGCCATTT 
GCAGCTCAGA TGGCAGGTCT GGATGAAAAA TCAGGACTTG GTAGTCAGAT GGGGCTGATG 
CCTGGTGCTG TGGGTCCAGT GGGTCCAAGA GGTCCTCAAG GTCTTCAAGG AAAACAAGGT 
GGTGCTGGCC CCACAGGCCC TCCTGGTGAA CCTGGAGATC CAGGACCTAT GGGTCCAGTT 
GGTTCACGTG GACCAGAAGG GCCTCCAGGC AAACCTGGTG AAGATGGTGA ACCCGGTAGA 
CCAGGACAGT CTGGTGAGCC TGGATTTCCA GGATCTCCAG GGCCTCGAGG CTTTCCTGGT 
GCTCCAGGTC TTCCAGGTCT CAAGGGTCAC AGGGGATTGA AAGGTCTCGA TGGTCCAAAA 
GGTGAAATTG GAGCAGCTGG AGCAAAGGGA GAATCTGGAC CAACAGGTGC CATGGGTGCA 
ACAGGTCCCC TAGGACCTAG AGGAATGCCA GGAGAAAGAG GTCGAATTGG ACCACAGGGT 
GCCCCTGGTG GACGTGGTCA GCATGGCATG CCTGGAAAGC CCGGGCCAAT GGGTCCCCTT 
GGCATAACTG GTTCTCCAGG TTTTCCAGGC GCTCCTGGTA TGAAGGGAGA AGCAGGTCCA 
ACCGGTCCTC GTGGTCCTGA AGGCCCTCAA GGACAAAGAG GTGAAACTGG TCCACAAGGC 
CCAGCTGGAT CACAAGGTCT TCCTGGTACG GAAGGGACAG ATGGCTCTCC AGGTGCCAAA 
GGCCCAACTG GATCTCCAGG CACTGGGGGC CCCCCTGGCT TATTGGGTCC ACCTGGTTCC 
CCAGGACCAC AAGGCAGTAC AGGCCCACCT GGTATTCGAG GGCAGCCGGG AGATCCTGGA 
GTTCCAGGTT TCAAGGGAGA AGCAGGACCC AAAGGTGAAC CAGGCCCGCA AGGTCCCCAA 
GGTCCCATTG GCCCCATAGG TGAAGAAGGG AAAAGAGGCC CTCGAGGTGA TCCAGGGTCA 
GTGGGTCCAC CAGGTCCAGT TGGAGAGAGG GGTGCTCCTG GGAACCGTGG TTTTCCAGGT 
TCTGATGGCT TGCCTGGACC AAAGGGTGCC CAAGGAGAGC GTGGTCCAGC AGGTTCTTCT 
GGTCCTAAAG GAGGTCAGGG AGATCCTGGG CGTCCTGGTG AGCCTGGACT CCCAGGTGCA 
AGGGGTTTGA CAGGAAATCC AGGTGTTCAA GGCCCTGAAG GAAAACTTGG CCCCTTGGGT 
GGCCCTGGAG AAGATGGACG TCCTGGTCCT GCAGGTTCGA TAGGAATCAG AGGTCAGCCA 
GGCAGTATGG GCCTTTCTGG GCCAAAGGGT AGTAGTGGTG ATCCTGGTAA ACCAGGAGAA 
GCAGGAAATG CAGGTGTTCC AGGACAAAGA GGAGCTCCTG GCAAAGACGG TGAAGTCGGT 
CCTTCTGGTC CTGTTGGCCC ACCAGGCCTG GCAGGAGAAA GAGGAGAACA AGGCCCTCCA 
GGTCCTACAG GGTTTCAGGG CTTACCTGGG CCTCCAGGTC CTCCTGGTGA AGGAGGAAAG 
CCAGGTGATC AGGGTGTTCC CGGAGATCCT GGAGTTGTTG GTCCATTGGG TCCCAGGGGA 
GAACGTGGCA ACCCGGGGGA ACGGGGAGAG CCAGGTGCAT CAGGACTTGC AGGTGAAAAG 
GGTATGGCTG GAGGTCATGG TCCTGATGGG CCAAAGGGAA GCCCAGGCCC TAGTGGGACG 
CCTGGTGATC CAGGCCCACC AGGTCTTCAA GGTATGCCTG GAGAAAGAGG GATTGCTGGA 
ACTCCTGGTC CAAAGGGTGA CAGAGGTGGT ATGGGTGAGA AAGGTGCTGA AGGTACTGCT 
GGCAATGATG GGGCAAGAGG TCTTCCTGGT CCAATAGGCC CAATGGGTCC AGCAGGGTCC 
ACTGGAGAAA AGGGTGAACC AGGTCCTCGA GGTCTTGTTG GTCCTCCCGG TTCTCGTGGA 
AACCCTGGTT CTCGAGGAGA AAATGGCCCA ACTGGTGCTG TTGGTTTTGC TGGTCCTCCA 
GGCCCTGATG GTCAGCCGGG TGTGAAAGGT GAACCTGGGG AACCTGGACA GAAAGGAGAT 
GCTGGATCTC CAGGACCACA GGGTCTTGCT GGATCTCCTG GTCCACCAGG TCCTTCAGGT 
GTTCCTGGTC TGAAAGGTGG TCGAGGAACC CAGGGTCCCC CTGGTGCTAC TGGATTCCCT 
GGATCTGCTG GAAGAGTTGG ACCTCCTGGA CCCACTGGAG CTCCAGGGCC TGCTGGGCCT 
ATTGGTGAGC CAGGAAAAGA AGGTCCCCCA GGTCTTCGTG GTGATCCTGG TTCTCATGGT 
CGTGTGGGTG ATCGAGGACC AGCTGGGCCC CCTGGTGGTC CTGGAGACAA GGGTGATGCA 
GGAGAAGATG GGCAACCGGG TCCAGATGGC CCCCCAGGTC CAGCAGGAAC TACTGGTCAA 
AGAGGTATTG TTGGTATGCC AGGACAGCGA GGGGAAAGAG GCATGCCAGG ACTGCCAGGT 
CCTGCAGGTA CACCAGGAAA ACAAGGTCCC ACAGGGCCAC CCGGTGATAA AGGTCCCCCA 
GGACCTATTG GCCAATCAGG TGCCACTGGA CCTGTAGGGG AAGCTGGTCC AGAAGGACCT 
GCTGGTAATG ATGGTACTCC AGGACGAGAT GGAGCTGTTG GAGAGCGTGG TGATCGTGGT 
GAACCTGGCC CTGCAGGCCT ACCTGGTTCT CCAGGAGGTC CTGGAACTCC AGGTCCTGTT 
GGCCCCCCGG GAGATGCAGG TCAAAGAGGT GAAACTGGTT CTCGAGGGCC AGTGGGTCCC 
CCAGGCCGTG CAGGAAAAAG AGGTTTGCCT GGTCCCCAAG GGCCTCGTGG TGACAAAGGT 
GACAATGGAG ATCGAGGTGA CAGGGGCCAG AAAGGACACA GAGGTTTCAC TGGGCTTCAA 
GGTCTTCCTG GTCCTCCTGG CCCTATTGGT GAACAGGGCA GTCCTGGCAT TCCAGGCCCA 
TTTGGTCCTC GGGGTCCTCC AGGTCCAGTT GGGCCTTCAG GCAAAGATGG AAGTCCTGGA 
CCACTAGGGC CGATTGGGCC TCCTGGTGTT AGAGGAAGTG TAGGAGAAGC TGGGCCTGAG 
GGTCCACCAG GTGATCCTGG CCCTCCTGGT CCCCCCGGCC CTCCAGGTCA CCTCACTGCT 
GCCATTGGTG ACATTATGGG ACACTATGAT GATGCTATGG CAGATCCACT ACCAGAGTTT 
ACTGAAGATG AAGCAGCTCC TGATGACCAC AATAAAACAG ACCCAGGAGT CCATGCCACG 
CTGAAGTCAC TGAGCAGCCA GATTGAGACT ATGCGCAGCC CAGATGGTTC GAAGAAACAC 
CCAGCACGGA CTTGTGATGA CCTGAAGCTG TGCCACCCAT CCAAGAAGAG TGGTGAATAT 
TGGATTGATC CTAATCAGGG ATGTGTTGAA GATGCTATAA AAGTATACTG CAACATGGAA 
ACTGGAGAGA CCTGCATTTC TGCAAATCCA TCAACTATCC CACGTAAAAC ATGGTGGGCC 
AGCAGATCAT CTGACTTGAA GCCCATTTGG TATGGCCTTG ATATGAACAG GGGATCACAG 
TTTGTGTATG GGGATACAGC TGTCACTCAA ATGACATTCC TGCGCCTCCT GTCAAAGGAA 
GCTTCCCAGA ATATCACTTA CCTCTGTAAG AACAGTGTTG GGTACATGGA TGACCAAACT 
AAGAACCTCA AGAAAGCTGT CATTCTGAAA GGGGCCAATG ACCTGGAGAT CAAGGCGGAG 
GGAAACAGCC GATTCAGATA CACTGTTCTT CAAGACAGCT GTTCTAAACG TAATGGCAAC 
GTGGGCAAGA CGGTCTTTGA GTACCGAACG CAGAACGTGG CACGGTTGCC TATCATAGAT 
ATCGCACCAG TGGATGTTGG CAGCACTGAC CAGGAATTTG GAATTGAAAT TGGGCCAGTT 
TGTTTTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A2 ( XM_015289203.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015144689.1 Gallus gallus collagen type V alpha 2 chain (COL5A2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa38196
Clone ID Related Accession (Same CDS sequence)	XM_015289203.2 , XM_015289203.1 , XM_025152272.1
Accession Version	XM_015289203.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	collagen alpha-2(V) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289203.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCTGA ACAGGGATAT TTGGAAGCCC TCACCGTGCC AGATTTGTGT CTGTGACAAC 
GGAGCTATTC TTTGCGATGA AATCCAGTGT CAAGATGTGT TGGAGTGTGA GAACCCACAG 
GTGCCCCCAG GAGAGTGCTG TCCAGTGTGT CCCCATACCG CACGTGATTT TGACCCTACC 
GTTGGCAGAG GAAGAAAGGG GCAAAAAGGA GAACCTGGAA TAGTGCCACC TGTTTCAGGA 
ATACGAGGCC GCCCAGGGCC TGCAGGACCT CCCGGTTCAC AGGGACCACG GGGAGAACGA 
GGACCTAAGG GAAGACCTGG TCCTCGTGGT CCTCAAGGGA TTGATGGAGA ACCCGGTATC 
CCTGGCCAGC CTGGTGACCC TGGTCCTCCT GGGCATCCAA CTCACACAGG ACCAGATGGG 
CTAAGCAGGC CATTTGCAGC TCAGATGGCA GGTCTGGATG AAAAATCAGG ACTTGGTAGT 
CAGATGGGGC TGATGCCTGG TGCTGTGGGT CCAGTGGGTC CAAGAGGTCC TCAAGGTCTT 
CAAGGAAAAC AAGGTGGTGC TGGCCCCACA GGCCCTCCTG GTGAACCTGG AGATCCAGGA 
CCTATGGGTC CAGTTGGTTC ACGTGGACCA GAAGGGCCTC CAGGCAAACC TGGTGAAGAT 
GGTGAACCCG GTAGACCAGG ACAGTCTGGT GAGCCTGGAT TTCCAGGATC TCCAGGGCCT 
CGAGGCTTTC CTGGTGCTCC AGGTCTTCCA GGTCTCAAGG GTCACAGGGG ATTGAAAGGT 
CTCGATGGTC CAAAAGGTGA AATTGGAGCA GCTGGAGCAA AGGGAGAATC TGGACCAACA 
GGTGCCATGG GTGCAACAGG TCCCCTAGGA CCTAGAGGAA TGCCAGGAGA AAGAGGTCGA 
ATTGGACCAC AGGGTGCCCC TGGTGGACGT GGTCAGCATG GCATGCCTGG AAAGCCCGGG 
CCAATGGGTC CCCTTGGCAT AACTGGTTCT CCAGGTTTTC CAGGCGCTCC TGGTATGAAG 
GGAGAAGCAG GTCCAACCGG TCCTCGTGGT CCTGAAGGCC CTCAAGGACA AAGAGGTGAA 
ACTGGTCCAC AAGGCCCAGC TGGATCACAA GGTCTTCCTG GTACGGAAGG GACAGATGGC 
TCTCCAGGTG CCAAAGGCCC AACTGGATCT CCAGGCACTG GGGGCCCCCC TGGCTTATTG 
GGTCCACCTG GTTCCCCAGG ACCACAAGGC AGTACAGGCC CACCTGGTAT TCGAGGGCAG 
CCGGGAGATC CTGGAGTTCC AGGTTTCAAG GGAGAAGCAG GACCCAAAGG TGAACCAGGC 
CCGCAAGGTC CCCAAGGTCC CATTGGCCCC ATAGGTGAAG AAGGGAAAAG AGGCCCTCGA 
GGTGATCCAG GGTCAGTGGG TCCACCAGGT CCAGTTGGAG AGAGGGGTGC TCCTGGGAAC 
CGTGGTTTTC CAGGTTCTGA TGGCTTGCCT GGACCAAAGG GTGCCCAAGG AGAGCGTGGT 
CCAGCAGGTT CTTCTGGTCC TAAAGGAGGT CAGGGAGATC CTGGGCGTCC TGGTGAGCCT 
GGACTCCCAG GTGCAAGGGG TTTGACAGGA AATCCAGGTG TTCAAGGCCC TGAAGGAAAA 
CTTGGCCCCT TGGGTGGCCC TGGAGAAGAT GGACGTCCTG GTCCTGCAGG TTCGATAGGA 
ATCAGAGGTC AGCCAGGCAG TATGGGCCTT TCTGGGCCAA AGGGTAGTAG TGGTGATCCT 
GGTAAACCAG GAGAAGCAGG AAATGCAGGT GTTCCAGGAC AAAGAGGAGC TCCTGGCAAA 
GACGGTGAAG TCGGTCCTTC TGGTCCTGTT GGCCCACCAG GCCTGGCAGG AGAAAGAGGA 
GAACAAGGCC CTCCAGGTCC TACAGGGTTT CAGGGCTTAC CTGGGCCTCC AGGTCCTCCT 
GGTGAAGGAG GAAAGCCAGG TGATCAGGGT GTTCCCGGAG ATCCTGGAGT TGTTGGTCCA 
TTGGGTCCCA GGGGAGAACG TGGCAACCCG GGGGAACGGG GAGAGCCAGG TGCATCAGGA 
CTTGCAGGTG AAAAGGGTAT GGCTGGAGGT CATGGTCCTG ATGGGCCAAA GGGAAGCCCA 
GGCCCTAGTG GGACGCCTGG TGATCCAGGC CCACCAGGTC TTCAAGGTAT GCCTGGAGAA 
AGAGGGATTG CTGGAACTCC TGGTCCAAAG GGTGACAGAG GTGGTATGGG TGAGAAAGGT 
GCTGAAGGTA CTGCTGGCAA TGATGGGGCA AGAGGTCTTC CTGGTCCAAT AGGCCCAATG 
GGTCCAGCAG GGTCCACTGG AGAAAAGGGT GAACCAGGTC CTCGAGGTCT TGTTGGTCCT 
CCCGGTTCTC GTGGAAACCC TGGTTCTCGA GGAGAAAATG GCCCAACTGG TGCTGTTGGT 
TTTGCTGGTC CTCCAGGCCC TGATGGTCAG CCGGGTGTGA AAGGTGAACC TGGGGAACCT 
GGACAGAAAG GAGATGCTGG ATCTCCAGGA CCACAGGGTC TTGCTGGATC TCCTGGTCCA 
CCAGGTCCTT CAGGTGTTCC TGGTCTGAAA GGTGGTCGAG GAACCCAGGG TCCCCCTGGT 
GCTACTGGAT TCCCTGGATC TGCTGGAAGA GTTGGACCTC CTGGACCCAC TGGAGCTCCA 
GGGCCTGCTG GGCCTATTGG TGAGCCAGGA AAAGAAGGTC CCCCAGGTCT TCGTGGTGAT 
CCTGGTTCTC ATGGTCGTGT GGGTGATCGA GGACCAGCTG GGCCCCCTGG TGGTCCTGGA 
GACAAGGGTG ATGCAGGAGA AGATGGGCAA CCGGGTCCAG ATGGCCCCCC AGGTCCAGCA 
GGAACTACTG GTCAAAGAGG TATTGTTGGT ATGCCAGGAC AGCGAGGGGA AAGAGGCATG 
CCAGGACTGC CAGGTCCTGC AGGTACACCA GGAAAACAAG GTCCCACAGG GCCACCCGGT 
GATAAAGGTC CCCCAGGACC TATTGGCCAA TCAGGTGCCA CTGGACCTGT AGGGGAAGCT 
GGTCCAGAAG GACCTGCTGG TAATGATGGT ACTCCAGGAC GAGATGGAGC TGTTGGAGAG 
CGTGGTGATC GTGGTGAACC TGGCCCTGCA GGCCTACCTG GTTCTCCAGG AGGTCCTGGA 
ACTCCAGGTC CTGTTGGCCC CCCGGGAGAT GCAGGTCAAA GAGGTGAAAC TGGTTCTCGA 
GGGCCAGTGG GTCCCCCAGG CCGTGCAGGA AAAAGAGGTT TGCCTGGTCC CCAAGGGCCT 
CGTGGTGACA AAGGTGACAA TGGAGATCGA GGTGACAGGG GCCAGAAAGG ACACAGAGGT 
TTCACTGGGC TTCAAGGTCT TCCTGGTCCT CCTGGCCCTA TTGGTGAACA GGGCAGTCCT 
GGCATTCCAG GCCCATTTGG TCCTCGGGGT CCTCCAGGTC CAGTTGGGCC TTCAGGCAAA 
GATGGAAGTC CTGGACCACT AGGGCCGATT GGGCCTCCTG GTGTTAGAGG AAGTGTAGGA 
GAAGCTGGGC CTGAGGGTCC ACCAGGTGAT CCTGGCCCTC CTGGTCCCCC CGGCCCTCCA 
GGTCACCTCA CTGCTGCCAT TGGTGACATT ATGGGACACT ATGATGATGC TATGGCAGAT 
CCACTACCAG AGTTTACTGA AGATGAAGCA GCTCCTGATG ACCACAATAA AACAGACCCA 
GGAGTCCATG CCACGCTGAA GTCACTGAGC AGCCAGATTG AGACTATGCG CAGCCCAGAT 
GGTTCGAAGA AACACCCAGC ACGGACTTGT GATGACCTGA AGCTGTGCCA CCCATCCAAG 
AAGAGTGGTG AATATTGGAT TGATCCTAAT CAGGGATGTG TTGAAGATGC TATAAAAGTA 
TACTGCAACA TGGAAACTGG AGAGACCTGC ATTTCTGCAA ATCCATCAAC TATCCCACGT 
AAAACATGGT GGGCCAGCAG ATCATCTGAC TTGAAGCCCA TTTGGTATGG CCTTGATATG 
AACAGGGGAT CACAGTTTGT GTATGGGGAT ACAGCTGTCA CTCAAATGAC ATTCCTGCGC 
CTCCTGTCAA AGGAAGCTTC CCAGAATATC ACTTACCTCT GTAAGAACAG TGTTGGGTAC 
ATGGATGACC AAACTAAGAA CCTCAAGAAA GCTGTCATTC TGAAAGGGGC CAATGACCTG 
GAGATCAAGG CGGAGGGAAA CAGCCGATTC AGATACACTG TTCTTCAAGA CAGCTGTTCT 
AAACGTAATG GCAACGTGGG CAAGACGGTC TTTGAGTACC GAACGCAGAA CGTGGCACGG 
TTGCCTATCA TAGATATCGC ACCAGTGGAT GTTGGCAGCA CTGACCAGGA ATTTGGAATT 
GAAATTGGGC CAGTTTGTTT TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015144689.1
CDS	438..4784
Translation	MYLNRDIWKPSPCQICVCDNGAILCDEIQCQDVLECENPQVPPGECCPVCPHTARDFDPTVGRGRKGQKGEPGIVPPVSGIRGRPGPAGPPGSQGPRGERGPKGRPGPRGPQGIDGEPGIPGQPGDPGPPGHPTHTGPDGLSRPFAAQMAGLDEKSGLGSQMGLMPGAVGPVGPRGPQGLQGKQGGAGPTGPPGEPGDPGPMGPVGSRGPEGPPGKPGEDGEPGRPGQSGEPGFPGSPGPRGFPGAPGLPGLKGHRGLKGLDGPKGEIGAAGAKGESGPTGAMGATGPLGPRGMPGERGRIGPQGAPGGRGQHGMPGKPGPMGPLGITGSPGFPGAPGMKGEAGPTGPRGPEGPQGQRGETGPQGPAGSQGLPGTEGTDGSPGAKGPTGSPGTGGPPGLLGPPGSPGPQGSTGPPGIRGQPGDPGVPGFKGEAGPKGEPGPQGPQGPIGPIGEEGKRGPRGDPGSVGPPGPVGERGAPGNRGFPGSDGLPGPKGAQGERGPAGSSGPKGGQGDPGRPGEPGLPGARGLTGNPGVQGPEGKLGPLGGPGEDGRPGPAGSIGIRGQPGSMGLSGPKGSSGDPGKPGEAGNAGVPGQRGAPGKDGEVGPSGPVGPPGLAGERGEQGPPGPTGFQGLPGPPGPPGEGGKPGDQGVPGDPGVVGPLGPRGERGNPGERGEPGASGLAGEKGMAGGHGPDGPKGSPGPSGTPGDPGPPGLQGMPGERGIAGTPGPKGDRGGMGEKGAEGTAGNDGARGLPGPIGPMGPAGSTGEKGEPGPRGLVGPPGSRGNPGSRGENGPTGAVGFAGPPGPDGQPGVKGEPGEPGQKGDAGSPGPQGLAGSPGPPGPSGVPGLKGGRGTQGPPGATGFPGSAGRVGPPGPTGAPGPAGPIGEPGKEGPPGLRGDPGSHGRVGDRGPAGPPGGPGDKGDAGEDGQPGPDGPPGPAGTTGQRGIVGMPGQRGERGMPGLPGPAGTPGKQGPTGPPGDKGPPGPIGQSGATGPVGEAGPEGPAGNDGTPGRDGAVGERGDRGEPGPAGLPGSPGGPGTPGPVGPPGDAGQRGETGSRGPVGPPGRAGKRGLPGPQGPRGDKGDNGDRGDRGQKGHRGFTGLQGLPGPPGPIGEQGSPGIPGPFGPRGPPGPVGPSGKDGSPGPLGPIGPPGVRGSVGEAGPEGPPGDPGPPGPPGPPGHLTAAIGDIMGHYDDAMADPLPEFTEDEAAPDDHNKTDPGVHATLKSLSSQIETMRSPDGSKKHPARTCDDLKLCHPSKKSGEYWIDPNQGCVEDAIKVYCNMETGETCISANPSTIPRKTWWASRSSDLKPIWYGLDMNRGSQFVYGDTAVTQMTFLRLLSKEASQNITYLCKNSVGYMDDQTKNLKKAVILKGANDLEIKAEGNSRFRYTVLQDSCSKRNGNVGKTVFEYRTQNVARLPIIDIAPVDVGSTDQEFGIEIGPVCFV

Target ORF information:

RefSeq Version	XM_015289203.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen type V alpha 2 chain (COL5A2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289203.2

ORF Insert Sequence:

ATGTACCTGA ACAGGGATAT TTGGAAGCCC TCACCGTGCC AGATTTGTGT CTGTGACAAC 
GGAGCTATTC TTTGCGATGA AATCCAGTGT CAAGATGTGT TGGAGTGTGA GAACCCACAG 
GTGCCCCCAG GAGAGTGCTG TCCAGTGTGT CCCCATACCG CACGTGATTT TGACCCTACC 
GTTGGCAGAG GAAGAAAGGG GCAAAAAGGA GAACCTGGAA TAGTGCCACC TGTTTCAGGA 
ATACGAGGCC GCCCAGGGCC TGCAGGACCT CCCGGTTCAC AGGGACCACG GGGAGAACGA 
GGACCTAAGG GAAGACCTGG TCCTCGTGGT CCTCAAGGGA TTGATGGAGA ACCCGGTATC 
CCTGGCCAGC CTGGTGACCC TGGTCCTCCT GGGCATCCAA CTCACACAGG ACCAGATGGG 
CTAAGCAGGC CATTTGCAGC TCAGATGGCA GGTCTGGATG AAAAATCAGG ACTTGGTAGT 
CAGATGGGGC TGATGCCTGG TGCTGTGGGT CCAGTGGGTC CAAGAGGTCC TCAAGGTCTT 
CAAGGAAAAC AAGGTGGTGC TGGCCCCACA GGCCCTCCTG GTGAACCTGG AGATCCAGGA 
CCTATGGGTC CAGTTGGTTC ACGTGGACCA GAAGGGCCTC CAGGCAAACC TGGTGAAGAT 
GGTGAACCCG GTAGACCAGG ACAGTCTGGT GAGCCTGGAT TTCCAGGATC TCCAGGGCCT 
CGAGGCTTTC CTGGTGCTCC AGGTCTTCCA GGTCTCAAGG GTCACAGGGG ATTGAAAGGT 
CTCGATGGTC CAAAAGGTGA AATTGGAGCA GCTGGAGCAA AGGGAGAATC TGGACCAACA 
GGTGCCATGG GTGCAACAGG TCCCCTAGGA CCTAGAGGAA TGCCAGGAGA AAGAGGTCGA 
ATTGGACCAC AGGGTGCCCC TGGTGGACGT GGTCAGCATG GCATGCCTGG AAAGCCCGGG 
CCAATGGGTC CCCTTGGCAT AACTGGTTCT CCAGGTTTTC CAGGCGCTCC TGGTATGAAG 
GGAGAAGCAG GTCCAACCGG TCCTCGTGGT CCTGAAGGCC CTCAAGGACA AAGAGGTGAA 
ACTGGTCCAC AAGGCCCAGC TGGATCACAA GGTCTTCCTG GTACGGAAGG GACAGATGGC 
TCTCCAGGTG CCAAAGGCCC AACTGGATCT CCAGGCACTG GGGGCCCCCC TGGCTTATTG 
GGTCCACCTG GTTCCCCAGG ACCACAAGGC AGTACAGGCC CACCTGGTAT TCGAGGGCAG 
CCGGGAGATC CTGGAGTTCC AGGTTTCAAG GGAGAAGCAG GACCCAAAGG TGAACCAGGC 
CCGCAAGGTC CCCAAGGTCC CATTGGCCCC ATAGGTGAAG AAGGGAAAAG AGGCCCTCGA 
GGTGATCCAG GGTCAGTGGG TCCACCAGGT CCAGTTGGAG AGAGGGGTGC TCCTGGGAAC 
CGTGGTTTTC CAGGTTCTGA TGGCTTGCCT GGACCAAAGG GTGCCCAAGG AGAGCGTGGT 
CCAGCAGGTT CTTCTGGTCC TAAAGGAGGT CAGGGAGATC CTGGGCGTCC TGGTGAGCCT 
GGACTCCCAG GTGCAAGGGG TTTGACAGGA AATCCAGGTG TTCAAGGCCC TGAAGGAAAA 
CTTGGCCCCT TGGGTGGCCC TGGAGAAGAT GGACGTCCTG GTCCTGCAGG TTCGATAGGA 
ATCAGAGGTC AGCCAGGCAG TATGGGCCTT TCTGGGCCAA AGGGTAGTAG TGGTGATCCT 
GGTAAACCAG GAGAAGCAGG AAATGCAGGT GTTCCAGGAC AAAGAGGAGC TCCTGGCAAA 
GACGGTGAAG TCGGTCCTTC TGGTCCTGTT GGCCCACCAG GCCTGGCAGG AGAAAGAGGA 
GAACAAGGCC CTCCAGGTCC TACAGGGTTT CAGGGCTTAC CTGGGCCTCC AGGTCCTCCT 
GGTGAAGGAG GAAAGCCAGG TGATCAGGGT GTTCCCGGAG ATCCTGGAGT TGTTGGTCCA 
TTGGGTCCCA GGGGAGAACG TGGCAACCCG GGGGAACGGG GAGAGCCAGG TGCATCAGGA 
CTTGCAGGTG AAAAGGGTAT GGCTGGAGGT CATGGTCCTG ATGGGCCAAA GGGAAGCCCA 
GGCCCTAGTG GGACGCCTGG TGATCCAGGC CCACCAGGTC TTCAAGGTAT GCCTGGAGAA 
AGAGGGATTG CTGGAACTCC TGGTCCAAAG GGTGACAGAG GTGGTATGGG TGAGAAAGGT 
GCTGAAGGTA CTGCTGGCAA TGATGGGGCA AGAGGTCTTC CTGGTCCAAT AGGCCCAATG 
GGTCCAGCAG GGTCCACTGG AGAAAAGGGT GAACCAGGTC CTCGAGGTCT TGTTGGTCCT 
CCCGGTTCTC GTGGAAACCC TGGTTCTCGA GGAGAAAATG GCCCAACTGG TGCTGTTGGT 
TTTGCTGGTC CTCCAGGCCC TGATGGTCAG CCGGGTGTGA AAGGTGAACC TGGGGAACCT 
GGACAGAAAG GAGATGCTGG ATCTCCAGGA CCACAGGGTC TTGCTGGATC TCCTGGTCCA 
CCAGGTCCTT CAGGTGTTCC TGGTCTGAAA GGTGGTCGAG GAACCCAGGG TCCCCCTGGT 
GCTACTGGAT TCCCTGGATC TGCTGGAAGA GTTGGACCTC CTGGACCCAC TGGAGCTCCA 
GGGCCTGCTG GGCCTATTGG TGAGCCAGGA AAAGAAGGTC CCCCAGGTCT TCGTGGTGAT 
CCTGGTTCTC ATGGTCGTGT GGGTGATCGA GGACCAGCTG GGCCCCCTGG TGGTCCTGGA 
GACAAGGGTG ATGCAGGAGA AGATGGGCAA CCGGGTCCAG ATGGCCCCCC AGGTCCAGCA 
GGAACTACTG GTCAAAGAGG TATTGTTGGT ATGCCAGGAC AGCGAGGGGA AAGAGGCATG 
CCAGGACTGC CAGGTCCTGC AGGTACACCA GGAAAACAAG GTCCCACAGG GCCACCCGGT 
GATAAAGGTC CCCCAGGACC TATTGGCCAA TCAGGTGCCA CTGGACCTGT AGGGGAAGCT 
GGTCCAGAAG GACCTGCTGG TAATGATGGT ACTCCAGGAC GAGATGGAGC TGTTGGAGAG 
CGTGGTGATC GTGGTGAACC TGGCCCTGCA GGCCTACCTG GTTCTCCAGG AGGTCCTGGA 
ACTCCAGGTC CTGTTGGCCC CCCGGGAGAT GCAGGTCAAA GAGGTGAAAC TGGTTCTCGA 
GGGCCAGTGG GTCCCCCAGG CCGTGCAGGA AAAAGAGGTT TGCCTGGTCC CCAAGGGCCT 
CGTGGTGACA AAGGTGACAA TGGAGATCGA GGTGACAGGG GCCAGAAAGG ACACAGAGGT 
TTCACTGGGC TTCAAGGTCT TCCTGGTCCT CCTGGCCCTA TTGGTGAACA GGGCAGTCCT 
GGCATTCCAG GCCCATTTGG TCCTCGGGGT CCTCCAGGTC CAGTTGGGCC TTCAGGCAAA 
GATGGAAGTC CTGGACCACT AGGGCCGATT GGGCCTCCTG GTGTTAGAGG AAGTGTAGGA 
GAAGCTGGGC CTGAGGGTCC ACCAGGTGAT CCTGGCCCTC CTGGTCCCCC CGGCCCTCCA 
GGTCACCTCA CTGCTGCCAT TGGTGACATT ATGGGACACT ATGATGATGC TATGGCAGAT 
CCACTACCAG AGTTTACTGA AGATGAAGCA GCTCCTGATG ACCACAATAA AACAGACCCA 
GGAGTCCATG CCACGCTGAA GTCACTGAGC AGCCAGATTG AGACTATGCG CAGCCCAGAT 
GGTTCGAAGA AACACCCAGC ACGGACTTGT GATGACCTGA AGCTGTGCCA CCCATCCAAG 
AAGAGTGGTG AATATTGGAT TGATCCTAAT CAGGGATGTG TTGAAGATGC TATAAAAGTA 
TACTGCAACA TGGAAACTGG AGAGACCTGC ATTTCTGCAA ATCCATCAAC TATCCCACGT 
AAAACATGGT GGGCCAGCAG ATCATCTGAC TTGAAGCCCA TTTGGTATGG CCTTGATATG 
AACAGGGGAT CACAGTTTGT GTATGGGGAT ACAGCTGTCA CTCAAATGAC ATTCCTGCGC 
CTCCTGTCAA AGGAAGCTTC CCAGAATATC ACTTACCTCT GTAAGAACAG TGTTGGGTAC 
ATGGATGACC AAACTAAGAA CCTCAAGAAA GCTGTCATTC TGAAAGGGGC CAATGACCTG 
GAGATCAAGG CGGAGGGAAA CAGCCGATTC AGATACACTG TTCTTCAAGA CAGCTGTTCT 
AAACGTAATG GCAACGTGGG CAAGACGGTC TTTGAGTACC GAACGCAGAA CGTGGCACGG 
TTGCCTATCA TAGATATCGC ACCAGTGGAT GTTGGCAGCA CTGACCAGGA ATTTGGAATT 
GAAATTGGGC CAGTTTGTTT TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A2 ( XM_015289203.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015144689.1 Gallus gallus collagen, type V, alpha 2 (COL5A2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa38196
Clone ID Related Accession (Same CDS sequence)	XM_015289203.2 , XM_015289203.1 , XM_025152272.1
Accession Version	XM_015289203.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	collagen alpha-2(V) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455901.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCTGA ACAGGGATAT TTGGAAGCCC TCACCGTGCC AGATTTGTGT CTGTGACAAC 
GGAGCTATTC TTTGCGATGA AATCCAGTGT CAAGATGTGT TGGAGTGTGA GAACCCACAG 
GTGCCCCCAG GAGAGTGCTG TCCAGTGTGT CCCCATACCG CACGTGATTT TGACCCTACC 
GTTGGCAGAG GAAGAAAGGG GCAAAAAGGA GAACCTGGAA TAGTGCCACC TGTTTCAGGA 
ATACGAGGCC GCCCAGGGCC TGCAGGACCT CCCGGTTCAC AGGGACCACG GGGAGAACGA 
GGACCTAAGG GAAGACCTGG TCCTCGTGGT CCTCAAGGGA TTGATGGAGA ACCCGGTATC 
CCTGGCCAGC CTGGTGACCC TGGTCCTCCT GGGCATCCAA CTCACACAGG ACCAGATGGG 
CTAAGCAGGC CATTTGCAGC TCAGATGGCA GGTCTGGATG AAAAATCAGG ACTTGGTAGT 
CAGATGGGGC TGATGCCTGG TGCTGTGGGT CCAGTGGGTC CAAGAGGTCC TCAAGGTCTT 
CAAGGAAAAC AAGGTGGTGC TGGCCCCACA GGCCCTCCTG GTGAACCTGG AGATCCAGGA 
CCTATGGGTC CAGTTGGTTC ACGTGGACCA GAAGGGCCTC CAGGCAAACC TGGTGAAGAT 
GGTGAACCCG GTAGACCAGG ACAGTCTGGT GAGCCTGGAT TTCCAGGATC TCCAGGGCCT 
CGAGGCTTTC CTGGTGCTCC AGGTCTTCCA GGTCTCAAGG GTCACAGGGG ATTGAAAGGT 
CTCGATGGTC CAAAAGGTGA AATTGGAGCA GCTGGAGCAA AGGGAGAATC TGGACCAACA 
GGTGCCATGG GTGCAACAGG TCCCCTAGGA CCTAGAGGAA TGCCAGGAGA AAGAGGTCGA 
ATTGGACCAC AGGGTGCCCC TGGTGGACGT GGTCAGCATG GCATGCCTGG AAAGCCCGGG 
CCAATGGGTC CCCTTGGCAT AACTGGTTCT CCAGGTTTTC CAGGCGCTCC TGGTATGAAG 
GGAGAAGCAG GTCCAACCGG TCCTCGTGGT CCTGAAGGCC CTCAAGGACA AAGAGGTGAA 
ACTGGTCCAC AAGGCCCAGC TGGATCACAA GGTCTTCCTG GTACGGAAGG GACAGATGGC 
TCTCCAGGTG CCAAAGGCCC AACTGGATCT CCAGGCACTG GGGGCCCCCC TGGCTTATTG 
GGTCCACCTG GTTCCCCAGG ACCACAAGGC AGTACAGGCC CACCTGGTAT TCGAGGGCAG 
CCGGGAGATC CTGGAGTTCC AGGTTTCAAG GGAGAAGCAG GACCCAAAGG TGAACCAGGC 
CCGCAAGGTC CCCAAGGTCC CATTGGCCCC ATAGGTGAAG AAGGGAAAAG AGGCCCTCGA 
GGTGATCCAG GGTCAGTGGG TCCACCAGGT CCAGTTGGAG AGAGGGGTGC TCCTGGGAAC 
CGTGGTTTTC CAGGTTCTGA TGGCTTGCCT GGACCAAAGG GTGCCCAAGG AGAGCGTGGT 
CCAGCAGGTT CTTCTGGTCC TAAAGGAGGT CAGGGAGATC CTGGGCGTCC TGGTGAGCCT 
GGACTCCCAG GTGCAAGGGG TTTGACAGGA AATCCAGGTG TTCAAGGCCC TGAAGGAAAA 
CTTGGCCCCT TGGGTGGCCC TGGAGAAGAT GGACGTCCTG GTCCTGCAGG TTCGATAGGA 
ATCAGAGGTC AGCCAGGCAG TATGGGCCTT TCTGGGCCAA AGGGTAGTAG TGGTGATCCT 
GGTAAACCAG GAGAAGCAGG AAATGCAGGT GTTCCAGGAC AAAGAGGAGC TCCTGGCAAA 
GACGGTGAAG TCGGTCCTTC TGGTCCTGTT GGCCCACCAG GCCTGGCAGG AGAAAGAGGA 
GAACAAGGCC CTCCAGGTCC TACAGGGTTT CAGGGCTTAC CTGGGCCTCC AGGTCCTCCT 
GGTGAAGGAG GAAAGCCAGG TGATCAGGGT GTTCCCGGAG ATCCTGGAGT TGTTGGTCCA 
TTGGGTCCCA GGGGAGAACG TGGCAACCCG GGGGAACGGG GAGAGCCAGG TGCATCAGGA 
CTTGCAGGTG AAAAGGGTAT GGCTGGAGGT CATGGTCCTG ATGGGCCAAA GGGAAGCCCA 
GGCCCTAGTG GGACGCCTGG TGATCCAGGC CCACCAGGTC TTCAAGGTAT GCCTGGAGAA 
AGAGGGATTG CTGGAACTCC TGGTCCAAAG GGTGACAGAG GTGGTATGGG TGAGAAAGGT 
GCTGAAGGTA CTGCTGGCAA TGATGGGGCA AGAGGTCTTC CTGGTCCAAT AGGCCCAATG 
GGTCCAGCAG GGTCCACTGG AGAAAAGGGT GAACCAGGTC CTCGAGGTCT TGTTGGTCCT 
CCCGGTTCTC GTGGAAACCC TGGTTCTCGA GGAGAAAATG GCCCAACTGG TGCTGTTGGT 
TTTGCTGGTC CTCCAGGCCC TGATGGTCAG CCGGGTGTGA AAGGTGAACC TGGGGAACCT 
GGACAGAAAG GAGATGCTGG ATCTCCAGGA CCACAGGGTC TTGCTGGATC TCCTGGTCCA 
CCAGGTCCTT CAGGTGTTCC TGGTCTGAAA GGTGGTCGAG GAACCCAGGG TCCCCCTGGT 
GCTACTGGAT TCCCTGGATC TGCTGGAAGA GTTGGACCTC CTGGACCCAC TGGAGCTCCA 
GGGCCTGCTG GGCCTATTGG TGAGCCAGGA AAAGAAGGTC CCCCAGGTCT TCGTGGTGAT 
CCTGGTTCTC ATGGTCGTGT GGGTGATCGA GGACCAGCTG GGCCCCCTGG TGGTCCTGGA 
GACAAGGGTG ATGCAGGAGA AGATGGGCAA CCGGGTCCAG ATGGCCCCCC AGGTCCAGCA 
GGAACTACTG GTCAAAGAGG TATTGTTGGT ATGCCAGGAC AGCGAGGGGA AAGAGGCATG 
CCAGGACTGC CAGGTCCTGC AGGTACACCA GGAAAACAAG GTCCCACAGG GCCACCCGGT 
GATAAAGGTC CCCCAGGACC TATTGGCCAA TCAGGTGCCA CTGGACCTGT AGGGGAAGCT 
GGTCCAGAAG GACCTGCTGG TAATGATGGT ACTCCAGGAC GAGATGGAGC TGTTGGAGAG 
CGTGGTGATC GTGGTGAACC TGGCCCTGCA GGCCTACCTG GTTCTCCAGG AGGTCCTGGA 
ACTCCAGGTC CTGTTGGCCC CCCGGGAGAT GCAGGTCAAA GAGGTGAAAC TGGTTCTCGA 
GGGCCAGTGG GTCCCCCAGG CCGTGCAGGA AAAAGAGGTT TGCCTGGTCC CCAAGGGCCT 
CGTGGTGACA AAGGTGACAA TGGAGATCGA GGTGACAGGG GCCAGAAAGG ACACAGAGGT 
TTCACTGGGC TTCAAGGTCT TCCTGGTCCT CCTGGCCCTA TTGGTGAACA GGGCAGTCCT 
GGCATTCCAG GCCCATTTGG TCCTCGGGGT CCTCCAGGTC CAGTTGGGCC TTCAGGCAAA 
GATGGAAGTC CTGGACCACT AGGGCCGATT GGGCCTCCTG GTGTTAGAGG AAGTGTAGGA 
GAAGCTGGGC CTGAGGGTCC ACCAGGTGAT CCTGGCCCTC CTGGTCCCCC CGGCCCTCCA 
GGTCACCTCA CTGCTGCCAT TGGTGACATT ATGGGACACT ATGATGATGC TATGGCAGAT 
CCACTACCAG AGTTTACTGA AGATGAAGCA GCTCCTGATG ACCACAATAA AACAGACCCA 
GGAGTCCATG CCACGCTGAA GTCACTGAGC AGCCAGATTG AGACTATGCG CAGCCCAGAT 
GGTTCGAAGA AACACCCAGC ACGGACTTGT GATGACCTGA AGCTGTGCCA CCCATCCAAG 
AAGAGTGGTG AATATTGGAT TGATCCTAAT CAGGGATGTG TTGAAGATGC TATAAAAGTA 
TACTGCAACA TGGAAACTGG AGAGACCTGC ATTTCTGCAA ATCCATCAAC TATCCCACGT 
AAAACATGGT GGGCCAGCAG ATCATCTGAC TTGAAGCCCA TTTGGTATGG CCTTGATATG 
AACAGGGGAT CACAGTTTGT GTATGGGGAT ACAGCTGTCA CTCAAATGAC ATTCCTGCGC 
CTCCTGTCAA AGGAAGCTTC CCAGAATATC ACTTACCTCT GTAAGAACAG TGTTGGGTAC 
ATGGATGACC AAACTAAGAA CCTCAAGAAA GCTGTCATTC TGAAAGGGGC CAATGACCTG 
GAGATCAAGG CGGAGGGAAA CAGCCGATTC AGATACACTG TTCTTCAAGA CAGCTGTTCT 
AAACGTAATG GCAACGTGGG CAAGACGGTC TTTGAGTACC GAACGCAGAA CGTGGCACGG 
TTGCCTATCA TAGATATCGC ACCAGTGGAT GTTGGCAGCA CTGACCAGGA ATTTGGAATT 
GAAATTGGGC CAGTTTGTTT TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015144689.1
CDS	430..4776
Translation	MYLNRDIWKPSPCQICVCDNGAILCDEIQCQDVLECENPQVPPGECCPVCPHTARDFDPTVGRGRKGQKGEPGIVPPVSGIRGRPGPAGPPGSQGPRGERGPKGRPGPRGPQGIDGEPGIPGQPGDPGPPGHPTHTGPDGLSRPFAAQMAGLDEKSGLGSQMGLMPGAVGPVGPRGPQGLQGKQGGAGPTGPPGEPGDPGPMGPVGSRGPEGPPGKPGEDGEPGRPGQSGEPGFPGSPGPRGFPGAPGLPGLKGHRGLKGLDGPKGEIGAAGAKGESGPTGAMGATGPLGPRGMPGERGRIGPQGAPGGRGQHGMPGKPGPMGPLGITGSPGFPGAPGMKGEAGPTGPRGPEGPQGQRGETGPQGPAGSQGLPGTEGTDGSPGAKGPTGSPGTGGPPGLLGPPGSPGPQGSTGPPGIRGQPGDPGVPGFKGEAGPKGEPGPQGPQGPIGPIGEEGKRGPRGDPGSVGPPGPVGERGAPGNRGFPGSDGLPGPKGAQGERGPAGSSGPKGGQGDPGRPGEPGLPGARGLTGNPGVQGPEGKLGPLGGPGEDGRPGPAGSIGIRGQPGSMGLSGPKGSSGDPGKPGEAGNAGVPGQRGAPGKDGEVGPSGPVGPPGLAGERGEQGPPGPTGFQGLPGPPGPPGEGGKPGDQGVPGDPGVVGPLGPRGERGNPGERGEPGASGLAGEKGMAGGHGPDGPKGSPGPSGTPGDPGPPGLQGMPGERGIAGTPGPKGDRGGMGEKGAEGTAGNDGARGLPGPIGPMGPAGSTGEKGEPGPRGLVGPPGSRGNPGSRGENGPTGAVGFAGPPGPDGQPGVKGEPGEPGQKGDAGSPGPQGLAGSPGPPGPSGVPGLKGGRGTQGPPGATGFPGSAGRVGPPGPTGAPGPAGPIGEPGKEGPPGLRGDPGSHGRVGDRGPAGPPGGPGDKGDAGEDGQPGPDGPPGPAGTTGQRGIVGMPGQRGERGMPGLPGPAGTPGKQGPTGPPGDKGPPGPIGQSGATGPVGEAGPEGPAGNDGTPGRDGAVGERGDRGEPGPAGLPGSPGGPGTPGPVGPPGDAGQRGETGSRGPVGPPGRAGKRGLPGPQGPRGDKGDNGDRGDRGQKGHRGFTGLQGLPGPPGPIGEQGSPGIPGPFGPRGPPGPVGPSGKDGSPGPLGPIGPPGVRGSVGEAGPEGPPGDPGPPGPPGPPGHLTAAIGDIMGHYDDAMADPLPEFTEDEAAPDDHNKTDPGVHATLKSLSSQIETMRSPDGSKKHPARTCDDLKLCHPSKKSGEYWIDPNQGCVEDAIKVYCNMETGETCISANPSTIPRKTWWASRSSDLKPIWYGLDMNRGSQFVYGDTAVTQMTFLRLLSKEASQNITYLCKNSVGYMDDQTKNLKKAVILKGANDLEIKAEGNSRFRYTVLQDSCSKRNGNVGKTVFEYRTQNVARLPIIDIAPVDVGSTDQEFGIEIGPVCFV

Target ORF information:

RefSeq Version	XM_015289203.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen, type V, alpha 2 (COL5A2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289203.1

ORF Insert Sequence:

ATGTACCTGA ACAGGGATAT TTGGAAGCCC TCACCGTGCC AGATTTGTGT CTGTGACAAC 
GGAGCTATTC TTTGCGATGA AATCCAGTGT CAAGATGTGT TGGAGTGTGA GAACCCACAG 
GTGCCCCCAG GAGAGTGCTG TCCAGTGTGT CCCCATACCG CACGTGATTT TGACCCTACC 
GTTGGCAGAG GAAGAAAGGG GCAAAAAGGA GAACCTGGAA TAGTGCCACC TGTTTCAGGA 
ATACGAGGCC GCCCAGGGCC TGCAGGACCT CCCGGTTCAC AGGGACCACG GGGAGAACGA 
GGACCTAAGG GAAGACCTGG TCCTCGTGGT CCTCAAGGGA TTGATGGAGA ACCCGGTATC 
CCTGGCCAGC CTGGTGACCC TGGTCCTCCT GGGCATCCAA CTCACACAGG ACCAGATGGG 
CTAAGCAGGC CATTTGCAGC TCAGATGGCA GGTCTGGATG AAAAATCAGG ACTTGGTAGT 
CAGATGGGGC TGATGCCTGG TGCTGTGGGT CCAGTGGGTC CAAGAGGTCC TCAAGGTCTT 
CAAGGAAAAC AAGGTGGTGC TGGCCCCACA GGCCCTCCTG GTGAACCTGG AGATCCAGGA 
CCTATGGGTC CAGTTGGTTC ACGTGGACCA GAAGGGCCTC CAGGCAAACC TGGTGAAGAT 
GGTGAACCCG GTAGACCAGG ACAGTCTGGT GAGCCTGGAT TTCCAGGATC TCCAGGGCCT 
CGAGGCTTTC CTGGTGCTCC AGGTCTTCCA GGTCTCAAGG GTCACAGGGG ATTGAAAGGT 
CTCGATGGTC CAAAAGGTGA AATTGGAGCA GCTGGAGCAA AGGGAGAATC TGGACCAACA 
GGTGCCATGG GTGCAACAGG TCCCCTAGGA CCTAGAGGAA TGCCAGGAGA AAGAGGTCGA 
ATTGGACCAC AGGGTGCCCC TGGTGGACGT GGTCAGCATG GCATGCCTGG AAAGCCCGGG 
CCAATGGGTC CCCTTGGCAT AACTGGTTCT CCAGGTTTTC CAGGCGCTCC TGGTATGAAG 
GGAGAAGCAG GTCCAACCGG TCCTCGTGGT CCTGAAGGCC CTCAAGGACA AAGAGGTGAA 
ACTGGTCCAC AAGGCCCAGC TGGATCACAA GGTCTTCCTG GTACGGAAGG GACAGATGGC 
TCTCCAGGTG CCAAAGGCCC AACTGGATCT CCAGGCACTG GGGGCCCCCC TGGCTTATTG 
GGTCCACCTG GTTCCCCAGG ACCACAAGGC AGTACAGGCC CACCTGGTAT TCGAGGGCAG 
CCGGGAGATC CTGGAGTTCC AGGTTTCAAG GGAGAAGCAG GACCCAAAGG TGAACCAGGC 
CCGCAAGGTC CCCAAGGTCC CATTGGCCCC ATAGGTGAAG AAGGGAAAAG AGGCCCTCGA 
GGTGATCCAG GGTCAGTGGG TCCACCAGGT CCAGTTGGAG AGAGGGGTGC TCCTGGGAAC 
CGTGGTTTTC CAGGTTCTGA TGGCTTGCCT GGACCAAAGG GTGCCCAAGG AGAGCGTGGT 
CCAGCAGGTT CTTCTGGTCC TAAAGGAGGT CAGGGAGATC CTGGGCGTCC TGGTGAGCCT 
GGACTCCCAG GTGCAAGGGG TTTGACAGGA AATCCAGGTG TTCAAGGCCC TGAAGGAAAA 
CTTGGCCCCT TGGGTGGCCC TGGAGAAGAT GGACGTCCTG GTCCTGCAGG TTCGATAGGA 
ATCAGAGGTC AGCCAGGCAG TATGGGCCTT TCTGGGCCAA AGGGTAGTAG TGGTGATCCT 
GGTAAACCAG GAGAAGCAGG AAATGCAGGT GTTCCAGGAC AAAGAGGAGC TCCTGGCAAA 
GACGGTGAAG TCGGTCCTTC TGGTCCTGTT GGCCCACCAG GCCTGGCAGG AGAAAGAGGA 
GAACAAGGCC CTCCAGGTCC TACAGGGTTT CAGGGCTTAC CTGGGCCTCC AGGTCCTCCT 
GGTGAAGGAG GAAAGCCAGG TGATCAGGGT GTTCCCGGAG ATCCTGGAGT TGTTGGTCCA 
TTGGGTCCCA GGGGAGAACG TGGCAACCCG GGGGAACGGG GAGAGCCAGG TGCATCAGGA 
CTTGCAGGTG AAAAGGGTAT GGCTGGAGGT CATGGTCCTG ATGGGCCAAA GGGAAGCCCA 
GGCCCTAGTG GGACGCCTGG TGATCCAGGC CCACCAGGTC TTCAAGGTAT GCCTGGAGAA 
AGAGGGATTG CTGGAACTCC TGGTCCAAAG GGTGACAGAG GTGGTATGGG TGAGAAAGGT 
GCTGAAGGTA CTGCTGGCAA TGATGGGGCA AGAGGTCTTC CTGGTCCAAT AGGCCCAATG 
GGTCCAGCAG GGTCCACTGG AGAAAAGGGT GAACCAGGTC CTCGAGGTCT TGTTGGTCCT 
CCCGGTTCTC GTGGAAACCC TGGTTCTCGA GGAGAAAATG GCCCAACTGG TGCTGTTGGT 
TTTGCTGGTC CTCCAGGCCC TGATGGTCAG CCGGGTGTGA AAGGTGAACC TGGGGAACCT 
GGACAGAAAG GAGATGCTGG ATCTCCAGGA CCACAGGGTC TTGCTGGATC TCCTGGTCCA 
CCAGGTCCTT CAGGTGTTCC TGGTCTGAAA GGTGGTCGAG GAACCCAGGG TCCCCCTGGT 
GCTACTGGAT TCCCTGGATC TGCTGGAAGA GTTGGACCTC CTGGACCCAC TGGAGCTCCA 
GGGCCTGCTG GGCCTATTGG TGAGCCAGGA AAAGAAGGTC CCCCAGGTCT TCGTGGTGAT 
CCTGGTTCTC ATGGTCGTGT GGGTGATCGA GGACCAGCTG GGCCCCCTGG TGGTCCTGGA 
GACAAGGGTG ATGCAGGAGA AGATGGGCAA CCGGGTCCAG ATGGCCCCCC AGGTCCAGCA 
GGAACTACTG GTCAAAGAGG TATTGTTGGT ATGCCAGGAC AGCGAGGGGA AAGAGGCATG 
CCAGGACTGC CAGGTCCTGC AGGTACACCA GGAAAACAAG GTCCCACAGG GCCACCCGGT 
GATAAAGGTC CCCCAGGACC TATTGGCCAA TCAGGTGCCA CTGGACCTGT AGGGGAAGCT 
GGTCCAGAAG GACCTGCTGG TAATGATGGT ACTCCAGGAC GAGATGGAGC TGTTGGAGAG 
CGTGGTGATC GTGGTGAACC TGGCCCTGCA GGCCTACCTG GTTCTCCAGG AGGTCCTGGA 
ACTCCAGGTC CTGTTGGCCC CCCGGGAGAT GCAGGTCAAA GAGGTGAAAC TGGTTCTCGA 
GGGCCAGTGG GTCCCCCAGG CCGTGCAGGA AAAAGAGGTT TGCCTGGTCC CCAAGGGCCT 
CGTGGTGACA AAGGTGACAA TGGAGATCGA GGTGACAGGG GCCAGAAAGG ACACAGAGGT 
TTCACTGGGC TTCAAGGTCT TCCTGGTCCT CCTGGCCCTA TTGGTGAACA GGGCAGTCCT 
GGCATTCCAG GCCCATTTGG TCCTCGGGGT CCTCCAGGTC CAGTTGGGCC TTCAGGCAAA 
GATGGAAGTC CTGGACCACT AGGGCCGATT GGGCCTCCTG GTGTTAGAGG AAGTGTAGGA 
GAAGCTGGGC CTGAGGGTCC ACCAGGTGAT CCTGGCCCTC CTGGTCCCCC CGGCCCTCCA 
GGTCACCTCA CTGCTGCCAT TGGTGACATT ATGGGACACT ATGATGATGC TATGGCAGAT 
CCACTACCAG AGTTTACTGA AGATGAAGCA GCTCCTGATG ACCACAATAA AACAGACCCA 
GGAGTCCATG CCACGCTGAA GTCACTGAGC AGCCAGATTG AGACTATGCG CAGCCCAGAT 
GGTTCGAAGA AACACCCAGC ACGGACTTGT GATGACCTGA AGCTGTGCCA CCCATCCAAG 
AAGAGTGGTG AATATTGGAT TGATCCTAAT CAGGGATGTG TTGAAGATGC TATAAAAGTA 
TACTGCAACA TGGAAACTGG AGAGACCTGC ATTTCTGCAA ATCCATCAAC TATCCCACGT 
AAAACATGGT GGGCCAGCAG ATCATCTGAC TTGAAGCCCA TTTGGTATGG CCTTGATATG 
AACAGGGGAT CACAGTTTGT GTATGGGGAT ACAGCTGTCA CTCAAATGAC ATTCCTGCGC 
CTCCTGTCAA AGGAAGCTTC CCAGAATATC ACTTACCTCT GTAAGAACAG TGTTGGGTAC 
ATGGATGACC AAACTAAGAA CCTCAAGAAA GCTGTCATTC TGAAAGGGGC CAATGACCTG 
GAGATCAAGG CGGAGGGAAA CAGCCGATTC AGATACACTG TTCTTCAAGA CAGCTGTTCT 
AAACGTAATG GCAACGTGGG CAAGACGGTC TTTGAGTACC GAACGCAGAA CGTGGCACGG 
TTGCCTATCA TAGATATCGC ACCAGTGGAT GTTGGCAGCA CTGACCAGGA ATTTGGAATT 
GAAATTGGGC CAGTTTGTTT TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A2 ( XM_025152272.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025008040.1 Gallus gallus collagen type V alpha 2 chain (COL5A2), transcript variant X4, mRNA.

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CloneID	OGa38196
Clone ID Related Accession (Same CDS sequence)	XM_015289203.2 , XM_015289203.1 , XM_025152272.1
Accession Version	XM_025152272.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	collagen alpha-2(V) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCTGA ACAGGGATAT TTGGAAGCCC TCACCGTGCC AGATTTGTGT CTGTGACAAC 
GGAGCTATTC TTTGCGATGA AATCCAGTGT CAAGATGTGT TGGAGTGTGA GAACCCACAG 
GTGCCCCCAG GAGAGTGCTG TCCAGTGTGT CCCCATACCG CACGTGATTT TGACCCTACC 
GTTGGCAGAG GAAGAAAGGG GCAAAAAGGA GAACCTGGAA TAGTGCCACC TGTTTCAGGA 
ATACGAGGCC GCCCAGGGCC TGCAGGACCT CCCGGTTCAC AGGGACCACG GGGAGAACGA 
GGACCTAAGG GAAGACCTGG TCCTCGTGGT CCTCAAGGGA TTGATGGAGA ACCCGGTATC 
CCTGGCCAGC CTGGTGACCC TGGTCCTCCT GGGCATCCAA CTCACACAGG ACCAGATGGG 
CTAAGCAGGC CATTTGCAGC TCAGATGGCA GGTCTGGATG AAAAATCAGG ACTTGGTAGT 
CAGATGGGGC TGATGCCTGG TGCTGTGGGT CCAGTGGGTC CAAGAGGTCC TCAAGGTCTT 
CAAGGAAAAC AAGGTGGTGC TGGCCCCACA GGCCCTCCTG GTGAACCTGG AGATCCAGGA 
CCTATGGGTC CAGTTGGTTC ACGTGGACCA GAAGGGCCTC CAGGCAAACC TGGTGAAGAT 
GGTGAACCCG GTAGACCAGG ACAGTCTGGT GAGCCTGGAT TTCCAGGATC TCCAGGGCCT 
CGAGGCTTTC CTGGTGCTCC AGGTCTTCCA GGTCTCAAGG GTCACAGGGG ATTGAAAGGT 
CTCGATGGTC CAAAAGGTGA AATTGGAGCA GCTGGAGCAA AGGGAGAATC TGGACCAACA 
GGTGCCATGG GTGCAACAGG TCCCCTAGGA CCTAGAGGAA TGCCAGGAGA AAGAGGTCGA 
ATTGGACCAC AGGGTGCCCC TGGTGGACGT GGTCAGCATG GCATGCCTGG AAAGCCCGGG 
CCAATGGGTC CCCTTGGCAT AACTGGTTCT CCAGGTTTTC CAGGCGCTCC TGGTATGAAG 
GGAGAAGCAG GTCCAACCGG TCCTCGTGGT CCTGAAGGCC CTCAAGGACA AAGAGGTGAA 
ACTGGTCCAC AAGGCCCAGC TGGATCACAA GGTCTTCCTG GTACGGAAGG GACAGATGGC 
TCTCCAGGTG CCAAAGGCCC AACTGGATCT CCAGGCACTG GGGGCCCCCC TGGCTTATTG 
GGTCCACCTG GTTCCCCAGG ACCACAAGGC AGTACAGGCC CACCTGGTAT TCGAGGGCAG 
CCGGGAGATC CTGGAGTTCC AGGTTTCAAG GGAGAAGCAG GACCCAAAGG TGAACCAGGC 
CCGCAAGGTC CCCAAGGTCC CATTGGCCCC ATAGGTGAAG AAGGGAAAAG AGGCCCTCGA 
GGTGATCCAG GGTCAGTGGG TCCACCAGGT CCAGTTGGAG AGAGGGGTGC TCCTGGGAAC 
CGTGGTTTTC CAGGTTCTGA TGGCTTGCCT GGACCAAAGG GTGCCCAAGG AGAGCGTGGT 
CCAGCAGGTT CTTCTGGTCC TAAAGGAGGT CAGGGAGATC CTGGGCGTCC TGGTGAGCCT 
GGACTCCCAG GTGCAAGGGG TTTGACAGGA AATCCAGGTG TTCAAGGCCC TGAAGGAAAA 
CTTGGCCCCT TGGGTGGCCC TGGAGAAGAT GGACGTCCTG GTCCTGCAGG TTCGATAGGA 
ATCAGAGGTC AGCCAGGCAG TATGGGCCTT TCTGGGCCAA AGGGTAGTAG TGGTGATCCT 
GGTAAACCAG GAGAAGCAGG AAATGCAGGT GTTCCAGGAC AAAGAGGAGC TCCTGGCAAA 
GACGGTGAAG TCGGTCCTTC TGGTCCTGTT GGCCCACCAG GCCTGGCAGG AGAAAGAGGA 
GAACAAGGCC CTCCAGGTCC TACAGGGTTT CAGGGCTTAC CTGGGCCTCC AGGTCCTCCT 
GGTGAAGGAG GAAAGCCAGG TGATCAGGGT GTTCCCGGAG ATCCTGGAGT TGTTGGTCCA 
TTGGGTCCCA GGGGAGAACG TGGCAACCCG GGGGAACGGG GAGAGCCAGG TGCATCAGGA 
CTTGCAGGTG AAAAGGGTAT GGCTGGAGGT CATGGTCCTG ATGGGCCAAA GGGAAGCCCA 
GGCCCTAGTG GGACGCCTGG TGATCCAGGC CCACCAGGTC TTCAAGGTAT GCCTGGAGAA 
AGAGGGATTG CTGGAACTCC TGGTCCAAAG GGTGACAGAG GTGGTATGGG TGAGAAAGGT 
GCTGAAGGTA CTGCTGGCAA TGATGGGGCA AGAGGTCTTC CTGGTCCAAT AGGCCCAATG 
GGTCCAGCAG GGTCCACTGG AGAAAAGGGT GAACCAGGTC CTCGAGGTCT TGTTGGTCCT 
CCCGGTTCTC GTGGAAACCC TGGTTCTCGA GGAGAAAATG GCCCAACTGG TGCTGTTGGT 
TTTGCTGGTC CTCCAGGCCC TGATGGTCAG CCGGGTGTGA AAGGTGAACC TGGGGAACCT 
GGACAGAAAG GAGATGCTGG ATCTCCAGGA CCACAGGGTC TTGCTGGATC TCCTGGTCCA 
CCAGGTCCTT CAGGTGTTCC TGGTCTGAAA GGTGGTCGAG GAACCCAGGG TCCCCCTGGT 
GCTACTGGAT TCCCTGGATC TGCTGGAAGA GTTGGACCTC CTGGACCCAC TGGAGCTCCA 
GGGCCTGCTG GGCCTATTGG TGAGCCAGGA AAAGAAGGTC CCCCAGGTCT TCGTGGTGAT 
CCTGGTTCTC ATGGTCGTGT GGGTGATCGA GGACCAGCTG GGCCCCCTGG TGGTCCTGGA 
GACAAGGGTG ATGCAGGAGA AGATGGGCAA CCGGGTCCAG ATGGCCCCCC AGGTCCAGCA 
GGAACTACTG GTCAAAGAGG TATTGTTGGT ATGCCAGGAC AGCGAGGGGA AAGAGGCATG 
CCAGGACTGC CAGGTCCTGC AGGTACACCA GGAAAACAAG GTCCCACAGG GCCACCCGGT 
GATAAAGGTC CCCCAGGACC TATTGGCCAA TCAGGTGCCA CTGGACCTGT AGGGGAAGCT 
GGTCCAGAAG GACCTGCTGG TAATGATGGT ACTCCAGGAC GAGATGGAGC TGTTGGAGAG 
CGTGGTGATC GTGGTGAACC TGGCCCTGCA GGCCTACCTG GTTCTCCAGG AGGTCCTGGA 
ACTCCAGGTC CTGTTGGCCC CCCGGGAGAT GCAGGTCAAA GAGGTGAAAC TGGTTCTCGA 
GGGCCAGTGG GTCCCCCAGG CCGTGCAGGA AAAAGAGGTT TGCCTGGTCC CCAAGGGCCT 
CGTGGTGACA AAGGTGACAA TGGAGATCGA GGTGACAGGG GCCAGAAAGG ACACAGAGGT 
TTCACTGGGC TTCAAGGTCT TCCTGGTCCT CCTGGCCCTA TTGGTGAACA GGGCAGTCCT 
GGCATTCCAG GCCCATTTGG TCCTCGGGGT CCTCCAGGTC CAGTTGGGCC TTCAGGCAAA 
GATGGAAGTC CTGGACCACT AGGGCCGATT GGGCCTCCTG GTGTTAGAGG AAGTGTAGGA 
GAAGCTGGGC CTGAGGGTCC ACCAGGTGAT CCTGGCCCTC CTGGTCCCCC CGGCCCTCCA 
GGTCACCTCA CTGCTGCCAT TGGTGACATT ATGGGACACT ATGATGATGC TATGGCAGAT 
CCACTACCAG AGTTTACTGA AGATGAAGCA GCTCCTGATG ACCACAATAA AACAGACCCA 
GGAGTCCATG CCACGCTGAA GTCACTGAGC AGCCAGATTG AGACTATGCG CAGCCCAGAT 
GGTTCGAAGA AACACCCAGC ACGGACTTGT GATGACCTGA AGCTGTGCCA CCCATCCAAG 
AAGAGTGGTG AATATTGGAT TGATCCTAAT CAGGGATGTG TTGAAGATGC TATAAAAGTA 
TACTGCAACA TGGAAACTGG AGAGACCTGC ATTTCTGCAA ATCCATCAAC TATCCCACGT 
AAAACATGGT GGGCCAGCAG ATCATCTGAC TTGAAGCCCA TTTGGTATGG CCTTGATATG 
AACAGGGGAT CACAGTTTGT GTATGGGGAT ACAGCTGTCA CTCAAATGAC ATTCCTGCGC 
CTCCTGTCAA AGGAAGCTTC CCAGAATATC ACTTACCTCT GTAAGAACAG TGTTGGGTAC 
ATGGATGACC AAACTAAGAA CCTCAAGAAA GCTGTCATTC TGAAAGGGGC CAATGACCTG 
GAGATCAAGG CGGAGGGAAA CAGCCGATTC AGATACACTG TTCTTCAAGA CAGCTGTTCT 
AAACGTAATG GCAACGTGGG CAAGACGGTC TTTGAGTACC GAACGCAGAA CGTGGCACGG 
TTGCCTATCA TAGATATCGC ACCAGTGGAT GTTGGCAGCA CTGACCAGGA ATTTGGAATT 
GAAATTGGGC CAGTTTGTTT TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025008040.1
CDS	323..4669
Translation	MYLNRDIWKPSPCQICVCDNGAILCDEIQCQDVLECENPQVPPGECCPVCPHTARDFDPTVGRGRKGQKGEPGIVPPVSGIRGRPGPAGPPGSQGPRGERGPKGRPGPRGPQGIDGEPGIPGQPGDPGPPGHPTHTGPDGLSRPFAAQMAGLDEKSGLGSQMGLMPGAVGPVGPRGPQGLQGKQGGAGPTGPPGEPGDPGPMGPVGSRGPEGPPGKPGEDGEPGRPGQSGEPGFPGSPGPRGFPGAPGLPGLKGHRGLKGLDGPKGEIGAAGAKGESGPTGAMGATGPLGPRGMPGERGRIGPQGAPGGRGQHGMPGKPGPMGPLGITGSPGFPGAPGMKGEAGPTGPRGPEGPQGQRGETGPQGPAGSQGLPGTEGTDGSPGAKGPTGSPGTGGPPGLLGPPGSPGPQGSTGPPGIRGQPGDPGVPGFKGEAGPKGEPGPQGPQGPIGPIGEEGKRGPRGDPGSVGPPGPVGERGAPGNRGFPGSDGLPGPKGAQGERGPAGSSGPKGGQGDPGRPGEPGLPGARGLTGNPGVQGPEGKLGPLGGPGEDGRPGPAGSIGIRGQPGSMGLSGPKGSSGDPGKPGEAGNAGVPGQRGAPGKDGEVGPSGPVGPPGLAGERGEQGPPGPTGFQGLPGPPGPPGEGGKPGDQGVPGDPGVVGPLGPRGERGNPGERGEPGASGLAGEKGMAGGHGPDGPKGSPGPSGTPGDPGPPGLQGMPGERGIAGTPGPKGDRGGMGEKGAEGTAGNDGARGLPGPIGPMGPAGSTGEKGEPGPRGLVGPPGSRGNPGSRGENGPTGAVGFAGPPGPDGQPGVKGEPGEPGQKGDAGSPGPQGLAGSPGPPGPSGVPGLKGGRGTQGPPGATGFPGSAGRVGPPGPTGAPGPAGPIGEPGKEGPPGLRGDPGSHGRVGDRGPAGPPGGPGDKGDAGEDGQPGPDGPPGPAGTTGQRGIVGMPGQRGERGMPGLPGPAGTPGKQGPTGPPGDKGPPGPIGQSGATGPVGEAGPEGPAGNDGTPGRDGAVGERGDRGEPGPAGLPGSPGGPGTPGPVGPPGDAGQRGETGSRGPVGPPGRAGKRGLPGPQGPRGDKGDNGDRGDRGQKGHRGFTGLQGLPGPPGPIGEQGSPGIPGPFGPRGPPGPVGPSGKDGSPGPLGPIGPPGVRGSVGEAGPEGPPGDPGPPGPPGPPGHLTAAIGDIMGHYDDAMADPLPEFTEDEAAPDDHNKTDPGVHATLKSLSSQIETMRSPDGSKKHPARTCDDLKLCHPSKKSGEYWIDPNQGCVEDAIKVYCNMETGETCISANPSTIPRKTWWASRSSDLKPIWYGLDMNRGSQFVYGDTAVTQMTFLRLLSKEASQNITYLCKNSVGYMDDQTKNLKKAVILKGANDLEIKAEGNSRFRYTVLQDSCSKRNGNVGKTVFEYRTQNVARLPIIDIAPVDVGSTDQEFGIEIGPVCFV

Target ORF information:

RefSeq Version	XM_025152272.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen type V alpha 2 chain (COL5A2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025152272.1

ORF Insert Sequence:

ATGTACCTGA ACAGGGATAT TTGGAAGCCC TCACCGTGCC AGATTTGTGT CTGTGACAAC 
GGAGCTATTC TTTGCGATGA AATCCAGTGT CAAGATGTGT TGGAGTGTGA GAACCCACAG 
GTGCCCCCAG GAGAGTGCTG TCCAGTGTGT CCCCATACCG CACGTGATTT TGACCCTACC 
GTTGGCAGAG GAAGAAAGGG GCAAAAAGGA GAACCTGGAA TAGTGCCACC TGTTTCAGGA 
ATACGAGGCC GCCCAGGGCC TGCAGGACCT CCCGGTTCAC AGGGACCACG GGGAGAACGA 
GGACCTAAGG GAAGACCTGG TCCTCGTGGT CCTCAAGGGA TTGATGGAGA ACCCGGTATC 
CCTGGCCAGC CTGGTGACCC TGGTCCTCCT GGGCATCCAA CTCACACAGG ACCAGATGGG 
CTAAGCAGGC CATTTGCAGC TCAGATGGCA GGTCTGGATG AAAAATCAGG ACTTGGTAGT 
CAGATGGGGC TGATGCCTGG TGCTGTGGGT CCAGTGGGTC CAAGAGGTCC TCAAGGTCTT 
CAAGGAAAAC AAGGTGGTGC TGGCCCCACA GGCCCTCCTG GTGAACCTGG AGATCCAGGA 
CCTATGGGTC CAGTTGGTTC ACGTGGACCA GAAGGGCCTC CAGGCAAACC TGGTGAAGAT 
GGTGAACCCG GTAGACCAGG ACAGTCTGGT GAGCCTGGAT TTCCAGGATC TCCAGGGCCT 
CGAGGCTTTC CTGGTGCTCC AGGTCTTCCA GGTCTCAAGG GTCACAGGGG ATTGAAAGGT 
CTCGATGGTC CAAAAGGTGA AATTGGAGCA GCTGGAGCAA AGGGAGAATC TGGACCAACA 
GGTGCCATGG GTGCAACAGG TCCCCTAGGA CCTAGAGGAA TGCCAGGAGA AAGAGGTCGA 
ATTGGACCAC AGGGTGCCCC TGGTGGACGT GGTCAGCATG GCATGCCTGG AAAGCCCGGG 
CCAATGGGTC CCCTTGGCAT AACTGGTTCT CCAGGTTTTC CAGGCGCTCC TGGTATGAAG 
GGAGAAGCAG GTCCAACCGG TCCTCGTGGT CCTGAAGGCC CTCAAGGACA AAGAGGTGAA 
ACTGGTCCAC AAGGCCCAGC TGGATCACAA GGTCTTCCTG GTACGGAAGG GACAGATGGC 
TCTCCAGGTG CCAAAGGCCC AACTGGATCT CCAGGCACTG GGGGCCCCCC TGGCTTATTG 
GGTCCACCTG GTTCCCCAGG ACCACAAGGC AGTACAGGCC CACCTGGTAT TCGAGGGCAG 
CCGGGAGATC CTGGAGTTCC AGGTTTCAAG GGAGAAGCAG GACCCAAAGG TGAACCAGGC 
CCGCAAGGTC CCCAAGGTCC CATTGGCCCC ATAGGTGAAG AAGGGAAAAG AGGCCCTCGA 
GGTGATCCAG GGTCAGTGGG TCCACCAGGT CCAGTTGGAG AGAGGGGTGC TCCTGGGAAC 
CGTGGTTTTC CAGGTTCTGA TGGCTTGCCT GGACCAAAGG GTGCCCAAGG AGAGCGTGGT 
CCAGCAGGTT CTTCTGGTCC TAAAGGAGGT CAGGGAGATC CTGGGCGTCC TGGTGAGCCT 
GGACTCCCAG GTGCAAGGGG TTTGACAGGA AATCCAGGTG TTCAAGGCCC TGAAGGAAAA 
CTTGGCCCCT TGGGTGGCCC TGGAGAAGAT GGACGTCCTG GTCCTGCAGG TTCGATAGGA 
ATCAGAGGTC AGCCAGGCAG TATGGGCCTT TCTGGGCCAA AGGGTAGTAG TGGTGATCCT 
GGTAAACCAG GAGAAGCAGG AAATGCAGGT GTTCCAGGAC AAAGAGGAGC TCCTGGCAAA 
GACGGTGAAG TCGGTCCTTC TGGTCCTGTT GGCCCACCAG GCCTGGCAGG AGAAAGAGGA 
GAACAAGGCC CTCCAGGTCC TACAGGGTTT CAGGGCTTAC CTGGGCCTCC AGGTCCTCCT 
GGTGAAGGAG GAAAGCCAGG TGATCAGGGT GTTCCCGGAG ATCCTGGAGT TGTTGGTCCA 
TTGGGTCCCA GGGGAGAACG TGGCAACCCG GGGGAACGGG GAGAGCCAGG TGCATCAGGA 
CTTGCAGGTG AAAAGGGTAT GGCTGGAGGT CATGGTCCTG ATGGGCCAAA GGGAAGCCCA 
GGCCCTAGTG GGACGCCTGG TGATCCAGGC CCACCAGGTC TTCAAGGTAT GCCTGGAGAA 
AGAGGGATTG CTGGAACTCC TGGTCCAAAG GGTGACAGAG GTGGTATGGG TGAGAAAGGT 
GCTGAAGGTA CTGCTGGCAA TGATGGGGCA AGAGGTCTTC CTGGTCCAAT AGGCCCAATG 
GGTCCAGCAG GGTCCACTGG AGAAAAGGGT GAACCAGGTC CTCGAGGTCT TGTTGGTCCT 
CCCGGTTCTC GTGGAAACCC TGGTTCTCGA GGAGAAAATG GCCCAACTGG TGCTGTTGGT 
TTTGCTGGTC CTCCAGGCCC TGATGGTCAG CCGGGTGTGA AAGGTGAACC TGGGGAACCT 
GGACAGAAAG GAGATGCTGG ATCTCCAGGA CCACAGGGTC TTGCTGGATC TCCTGGTCCA 
CCAGGTCCTT CAGGTGTTCC TGGTCTGAAA GGTGGTCGAG GAACCCAGGG TCCCCCTGGT 
GCTACTGGAT TCCCTGGATC TGCTGGAAGA GTTGGACCTC CTGGACCCAC TGGAGCTCCA 
GGGCCTGCTG GGCCTATTGG TGAGCCAGGA AAAGAAGGTC CCCCAGGTCT TCGTGGTGAT 
CCTGGTTCTC ATGGTCGTGT GGGTGATCGA GGACCAGCTG GGCCCCCTGG TGGTCCTGGA 
GACAAGGGTG ATGCAGGAGA AGATGGGCAA CCGGGTCCAG ATGGCCCCCC AGGTCCAGCA 
GGAACTACTG GTCAAAGAGG TATTGTTGGT ATGCCAGGAC AGCGAGGGGA AAGAGGCATG 
CCAGGACTGC CAGGTCCTGC AGGTACACCA GGAAAACAAG GTCCCACAGG GCCACCCGGT 
GATAAAGGTC CCCCAGGACC TATTGGCCAA TCAGGTGCCA CTGGACCTGT AGGGGAAGCT 
GGTCCAGAAG GACCTGCTGG TAATGATGGT ACTCCAGGAC GAGATGGAGC TGTTGGAGAG 
CGTGGTGATC GTGGTGAACC TGGCCCTGCA GGCCTACCTG GTTCTCCAGG AGGTCCTGGA 
ACTCCAGGTC CTGTTGGCCC CCCGGGAGAT GCAGGTCAAA GAGGTGAAAC TGGTTCTCGA 
GGGCCAGTGG GTCCCCCAGG CCGTGCAGGA AAAAGAGGTT TGCCTGGTCC CCAAGGGCCT 
CGTGGTGACA AAGGTGACAA TGGAGATCGA GGTGACAGGG GCCAGAAAGG ACACAGAGGT 
TTCACTGGGC TTCAAGGTCT TCCTGGTCCT CCTGGCCCTA TTGGTGAACA GGGCAGTCCT 
GGCATTCCAG GCCCATTTGG TCCTCGGGGT CCTCCAGGTC CAGTTGGGCC TTCAGGCAAA 
GATGGAAGTC CTGGACCACT AGGGCCGATT GGGCCTCCTG GTGTTAGAGG AAGTGTAGGA 
GAAGCTGGGC CTGAGGGTCC ACCAGGTGAT CCTGGCCCTC CTGGTCCCCC CGGCCCTCCA 
GGTCACCTCA CTGCTGCCAT TGGTGACATT ATGGGACACT ATGATGATGC TATGGCAGAT 
CCACTACCAG AGTTTACTGA AGATGAAGCA GCTCCTGATG ACCACAATAA AACAGACCCA 
GGAGTCCATG CCACGCTGAA GTCACTGAGC AGCCAGATTG AGACTATGCG CAGCCCAGAT 
GGTTCGAAGA AACACCCAGC ACGGACTTGT GATGACCTGA AGCTGTGCCA CCCATCCAAG 
AAGAGTGGTG AATATTGGAT TGATCCTAAT CAGGGATGTG TTGAAGATGC TATAAAAGTA 
TACTGCAACA TGGAAACTGG AGAGACCTGC ATTTCTGCAA ATCCATCAAC TATCCCACGT 
AAAACATGGT GGGCCAGCAG ATCATCTGAC TTGAAGCCCA TTTGGTATGG CCTTGATATG 
AACAGGGGAT CACAGTTTGT GTATGGGGAT ACAGCTGTCA CTCAAATGAC ATTCCTGCGC 
CTCCTGTCAA AGGAAGCTTC CCAGAATATC ACTTACCTCT GTAAGAACAG TGTTGGGTAC 
ATGGATGACC AAACTAAGAA CCTCAAGAAA GCTGTCATTC TGAAAGGGGC CAATGACCTG 
GAGATCAAGG CGGAGGGAAA CAGCCGATTC AGATACACTG TTCTTCAAGA CAGCTGTTCT 
AAACGTAATG GCAACGTGGG CAAGACGGTC TTTGAGTACC GAACGCAGAA CGTGGCACGG 
TTGCCTATCA TAGATATCGC ACCAGTGGAT GTTGGCAGCA CTGACCAGGA ATTTGGAATT 
GAAATTGGGC CAGTTTGTTT TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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電話

03-6811-6572

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050-3174-7589(受注専用)

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オンライン注文

COL5A2 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム