PMEL cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	PMEL
Entrez Gene ID	396007
Full Name	premelanosome protein
Synonyms	MMP115,SILV
General protein information	Preferred Names premelanosome protein Names melanocyte protein PMEL 115 kDa melanosomal matrix protein PMEL17 protein melanocyte protein Pmel 17 silver homolog silver locus protein homolog
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_205112.2	NP_990443.2	melanocyte protein PMEL precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pmelb	NP_001029090.2
Danio rerio (zebrafish)	pmela	NP_001038795.1
Homo sapiens (human)	PMEL	NP_001186983.1
Pan troglodytes (chimpanzee)	PMEL	XP_001169751.2
	PMEL	XP_001113158.1
Gallus gallus (chicken)	PMEL	NP_990443.2
Canis lupus familiaris (dog)	PMEL	NP_001096686.1
Bos taurus (cattle)	PMEL	NP_001073684.2
Mus musculus (house mouse)	Pmel	NP_068682.2
Rattus norvegicus (Norway rat)	Pmel	NP_001102199.1
Xenopus tropicalis (tropical clawed frog)	pmel	XP_004911936.1

Gene Ontology
Bibliography

Related articles in PubMed

Genotype at the PMEL17 locus affects social and explorative behaviour in chickens.
Karlsson AC, Kerje S, Andersson L, Jensen P
British poultry science51(2)170-7(2010 Apr)

A mutation within the transmembrane domain of melanosomal protein Silver (Pmel17) changes lumenal fragment interactions.
Kuliawat R, Santambrogio L
European journal of cell biology88(11)653-67(2009 Nov)

The Dominant white mutation in the PMEL17 gene does not cause visual impairment in chickens.
Karlsson AC, Kerje S, Hallb??k F, Jensen P
Veterinary ophthalmology12(5)292-8(2009 Sep-Oct)

Identification of breed-specific DNA polymorphisms for a simple and unambiguous screening system in germline chimeric chickens.
Choi JW, Lee EY, Shin JH, Zheng Y, Cho BW, Kim JK, Kim H, Han JY
Journal of experimental zoology. Part A, Ecological genetics and physiology307(4)241-8(2007 Apr)

Plumage color and feather pecking--behavioral differences associated with PMEL17 genotypes in chicken (Gallus gallus).
N?tt D, Kerje S, Andersson L, Jensen P
Behavior genetics37(2)399-407(2007 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The study strongly suggests a correlated effect between plumage colour and behaviour, and we conclude that PMEL17 may have a pleiotropic effect on social and explorative behaviour in chickens.
Title: Genotype at the PMEL17 locus affects social and explorative behaviour in chickens.

the transmembrane region may contribute to the structural integrity of Silver/Pmel17 oligomers or influence toxic, amyloidogenic properties
Title: A mutation within the transmembrane domain of melanosomal protein Silver (Pmel17) changes lumenal fragment interactions.

Dominant white mutation does not cause visual impairment
Title: The Dominant white mutation in the PMEL17 gene does not cause visual impairment in chickens.

In addition to the 9-bp insertion, WLs and KOCs have unique bases in single nucleotide polymorphisms (SNPs) at the 1,777th and 3,118th bases in the PMEL17 gene.
Title: Identification of breed-specific DNA polymorphisms for a simple and unambiguous screening system in germline chimeric chickens.

Genotype of PMEL17 affects plumage color and feather pecking behavior.
Title: Plumage color and feather pecking--behavioral differences associated with PMEL17 genotypes in chicken (Gallus gallus).

The following PMEL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PMEL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa27055	NM_205112.2 Latest version!	Gallus gallus premelanosome protein (PMEL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PMEL ( NM_205112.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990443.2 Gallus gallus premelanosome protein (PMEL), mRNA.

Price & Availability↑

CloneID	OGa27055
Clone ID Related Accession (Same CDS sequence)	NM_205112.2
Accession Version	NM_205112.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Gallus gallus(chicken)
Product	melanocyte protein PMEL precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D88348.2. On Nov 22, 2004 this sequence version replaced NM_205112.1. ##Evidence-Data-START## Transcript exon combination :: D88348.2 [ECO:0000332] ##Evidence-Data-END##

ATGCGGTTGC ACGGGGCCAT CGTGCTGCTG GCGGCACTGC TGGCCCTGGT CACGGCACAG 
CAGCGAGGCG GTGGCCGGAG CCGCGGTGGC GTTAAGGGCT CGGCGTGGGG TGGCCGCCCG 
GCGCCGTTCC GGAGCTGGGA TACAGCGCGG TACCGCCCAT GGCAGGAGGG GACGGCGCGG 
CAGAACGACT GCTGGAGAGG CGGCGACGTC ACCTTCGACA TCAGCAACGA TGCTCCCACC 
CTGGTGGGCG CCAGGGCCAC CTTCTCCATC GCTCTGCGCT TCCCCGGCAC GCAGACGGTG 
CTGCCCGACG GCCGCGTGGT GTGGAGCCAG AACTGCACCG TCAATGGCAC CCGCATGCTA 
CAGGGGGACC CGGTGTACCC AGAGCAGCTG GCCGAGGGCT CGGATGGAGT CTTCCCCGAC 
GGGCAGCCCT TTCCCCGCAG CGCCTGGGGC AAACGTGGGA GATTTGTCTA CGTGTGGTGG 
ACGTGGGGGC GCTACTGGCA GGTGGTGGAC GGAGCGACGT CGCAGCTGAC GGTGGGGACA 
GACGGGGTGG CCCTGGGCTC CTACACCATG GAGGTGGTGG TTTATCACTA CCGCGGCCGC 
CAGAGGTTCA TCCCCATCGG CCACGCCAGC ACGCAGTTCA GCATCACCGA CCAGGTGCCC 
ATTGCGGTGG ATGTGACACA GCTGGAGGTG GCAGCGGGGG ACGGCGGGAG CTTCGTGCGC 
AACCGCCCCG TGGCCTTCAA CGTGAGGCTG CACGACCCCA GCCATTACCT GCGTGATGCT 
GACATCTCCT ATTCGTGGGA CTTCGGGGAC CAGAGCGGCA CACTCATCTC CCGCAGCCCC 
ACCGTCACCC ACACCTACCT GCAGGCTGGT TCCTTTGCTG CCCGCCTGGT GCTGCAGGCA 
GCCATCCCGC TCAGCTCCTG CGGCACCTCC GCACCCCCTG TTGTGGACCC CACCACTGGG 
CCGGTGCCCT CCTTGGGACC CACGGCCACA CAGCCTGTGG GACCCACCGG ATCCGGCACT 
GCCACGGCAC CCAGCAACCT CACGGGATCC GGTACTGCTG CAGCACCCGG AACCACTGCA 
GCACCCAGAG CCTCCGGGGC ACCAGCAGAA CCCACGGGGG TCTCAGTGGC TGTGCTATCA 
GACAGCGCTG CCACTGAGCC CCTCCCCGAC CCCGTGCTCA GCACCGCAGT GGCCGATGCA 
GCAGCCGGTA CAGACCCCAC TGCAGACCCC CTGCCCCCCA CCTCAGTGTC CTCAGGTGGT 
GATGCTCCGG GCACTGTGGC CCCCACAGCA GTGGAGGGAA GCGTGGCAGC AGGTGTGGGG 
ACAGCGGAGG ATGTGGCCGC AGCCACCCCT GGAGCCACTG CTGCGGACGT GGCTGTGGAT 
ACAGCTGGAG CCACGGATGG GGATGCAGTT GGACCCACAG CTGCAGCTAC AGCTGAATCC 
ATAGCAGATC CCACAGCTGG AGCCACGGAT GGGGATGCAG TTGGAGCTAC AGCTGAATCC 
ATAGCAGATC CCACAGCTGG AGCCACGGAT GGGGACGCAG TTGGACCCAC AGCTGCAGCT 
ACAGCTGAAT CCATAGCAGA TCCCACAGCT GGAGCCACAG CTGTATCTTC TGGCAGTGCC 
ACAGCTGGAG CCACAGCGGA GCCGCTGCTG CTGGTGAAGC GCCAGGCGCC CGAGGCTGAG 
CCCACCGGCT GCGTCCTGTA CCGCTATGGC ACCTTCTCCA CTGAGCTCAA CATCGTCCAG 
GGCATCGAGA GCGTGGCCAT CGTGCAGGTG GTGCCCGCGG CGCCCGAGGG CAGCGGGAAC 
AGCGTGGAGC TGACGGTGAC ATGCGAGGGG AGCCTTCCGG AGGAGGTGTG CACCGTGGTG 
GCGGACGCCG AGTGCCGCAC AGCGCAGATG CAGACGTGCT CAGCCGTGGC GCCGGCGCCG 
GGCTGCCAGC TGGTGCTGCG CCAGGACTTC AACCAGTCCG GCCTCTACTG CCTCAACGTC 
TCGTTGGCCA ACGGCAACGG CTTGGCTGTG GCCAGCACCC ACGTGGCTGT CGGAGGAGCA 
TCCCCAGCCG CCAGTGGCAC CACGCTCACC GTGGGGCTGC TCTGGGCACC GCTCATGGCC 
GCTGCTCTGG GCACCGCTGC CTACACCTAC CGCCGTGTGA AGTACAGCCC GCTGCTGCCC 
ACAGCCCCCA CGGCCCCCCG GCCCCACAGC TGGCTGCCCC CCGGTGCTAC CCTGCGTTTG 
CTGCTGCGCC AGGCTTTTGG GGGGGCTCCC AGTGGAGAAA GCAGCCCCCT GCTGCGTGCC 
AACGCTGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990443.2
CDS	58..2349
Translation	MRLHGAIVLLAALLALVTAQQRGGGRSRGGVKGSAWGGRPAPFRSWDTARYRPWQEGTARQNDCWRGGDVTFDISNDAPTLVGARATFSIALRFPGTQTVLPDGRVVWSQNCTVNGTRMLQGDPVYPEQLAEGSDGVFPDGQPFPRSAWGKRGRFVYVWWTWGRYWQVVDGATSQLTVGTDGVALGSYTMEVVVYHYRGRQRFIPIGHASTQFSITDQVPIAVDVTQLEVAAGDGGSFVRNRPVAFNVRLHDPSHYLRDADISYSWDFGDQSGTLISRSPTVTHTYLQAGSFAARLVLQAAIPLSSCGTSAPPVVDPTTGPVPSLGPTATQPVGPTGSGTATAPSNLTGSGTAAAPGTTAAPRASGAPAEPTGVSVAVLSDSAATEPLPDPVLSTAVADAAAGTDPTADPLPPTSVSSGGDAPGTVAPTAVEGSVAAGVGTAEDVAAATPGATAADVAVDTAGATDGDAVGPTAAATAESIADPTAGATDGDAVGATAESIADPTAGATDGDAVGPTAAATAESIADPTAGATAVSSGSATAGATAEPLLLVKRQAPEAEPTGCVLYRYGTFSTELNIVQGIESVAIVQVVPAAPEGSGNSVELTVTCEGSLPEEVCTVVADAECRTAQMQTCSAVAPAPGCQLVLRQDFNQSGLYCLNVSLANGNGLAVASTHVAVGGASPAASGTTLTVGLLWAPLMAAALGTAAYTYRRVKYSPLLPTAPTAPRPHSWLPPGATLRLLLRQAFGGAPSGESSPLLRANAV

Target ORF information:

RefSeq Version	NM_205112.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus premelanosome protein (PMEL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205112.2

ORF Insert Sequence:

ATGCGGTTGC ACGGGGCCAT CGTGCTGCTG GCGGCACTGC TGGCCCTGGT CACGGCACAG 
CAGCGAGGCG GTGGCCGGAG CCGCGGTGGC GTTAAGGGCT CGGCGTGGGG TGGCCGCCCG 
GCGCCGTTCC GGAGCTGGGA TACAGCGCGG TACCGCCCAT GGCAGGAGGG GACGGCGCGG 
CAGAACGACT GCTGGAGAGG CGGCGACGTC ACCTTCGACA TCAGCAACGA TGCTCCCACC 
CTGGTGGGCG CCAGGGCCAC CTTCTCCATC GCTCTGCGCT TCCCCGGCAC GCAGACGGTG 
CTGCCCGACG GCCGCGTGGT GTGGAGCCAG AACTGCACCG TCAATGGCAC CCGCATGCTA 
CAGGGGGACC CGGTGTACCC AGAGCAGCTG GCCGAGGGCT CGGATGGAGT CTTCCCCGAC 
GGGCAGCCCT TTCCCCGCAG CGCCTGGGGC AAACGTGGGA GATTTGTCTA CGTGTGGTGG 
ACGTGGGGGC GCTACTGGCA GGTGGTGGAC GGAGCGACGT CGCAGCTGAC GGTGGGGACA 
GACGGGGTGG CCCTGGGCTC CTACACCATG GAGGTGGTGG TTTATCACTA CCGCGGCCGC 
CAGAGGTTCA TCCCCATCGG CCACGCCAGC ACGCAGTTCA GCATCACCGA CCAGGTGCCC 
ATTGCGGTGG ATGTGACACA GCTGGAGGTG GCAGCGGGGG ACGGCGGGAG CTTCGTGCGC 
AACCGCCCCG TGGCCTTCAA CGTGAGGCTG CACGACCCCA GCCATTACCT GCGTGATGCT 
GACATCTCCT ATTCGTGGGA CTTCGGGGAC CAGAGCGGCA CACTCATCTC CCGCAGCCCC 
ACCGTCACCC ACACCTACCT GCAGGCTGGT TCCTTTGCTG CCCGCCTGGT GCTGCAGGCA 
GCCATCCCGC TCAGCTCCTG CGGCACCTCC GCACCCCCTG TTGTGGACCC CACCACTGGG 
CCGGTGCCCT CCTTGGGACC CACGGCCACA CAGCCTGTGG GACCCACCGG ATCCGGCACT 
GCCACGGCAC CCAGCAACCT CACGGGATCC GGTACTGCTG CAGCACCCGG AACCACTGCA 
GCACCCAGAG CCTCCGGGGC ACCAGCAGAA CCCACGGGGG TCTCAGTGGC TGTGCTATCA 
GACAGCGCTG CCACTGAGCC CCTCCCCGAC CCCGTGCTCA GCACCGCAGT GGCCGATGCA 
GCAGCCGGTA CAGACCCCAC TGCAGACCCC CTGCCCCCCA CCTCAGTGTC CTCAGGTGGT 
GATGCTCCGG GCACTGTGGC CCCCACAGCA GTGGAGGGAA GCGTGGCAGC AGGTGTGGGG 
ACAGCGGAGG ATGTGGCCGC AGCCACCCCT GGAGCCACTG CTGCGGACGT GGCTGTGGAT 
ACAGCTGGAG CCACGGATGG GGATGCAGTT GGACCCACAG CTGCAGCTAC AGCTGAATCC 
ATAGCAGATC CCACAGCTGG AGCCACGGAT GGGGATGCAG TTGGAGCTAC AGCTGAATCC 
ATAGCAGATC CCACAGCTGG AGCCACGGAT GGGGACGCAG TTGGACCCAC AGCTGCAGCT 
ACAGCTGAAT CCATAGCAGA TCCCACAGCT GGAGCCACAG CTGTATCTTC TGGCAGTGCC 
ACAGCTGGAG CCACAGCGGA GCCGCTGCTG CTGGTGAAGC GCCAGGCGCC CGAGGCTGAG 
CCCACCGGCT GCGTCCTGTA CCGCTATGGC ACCTTCTCCA CTGAGCTCAA CATCGTCCAG 
GGCATCGAGA GCGTGGCCAT CGTGCAGGTG GTGCCCGCGG CGCCCGAGGG CAGCGGGAAC 
AGCGTGGAGC TGACGGTGAC ATGCGAGGGG AGCCTTCCGG AGGAGGTGTG CACCGTGGTG 
GCGGACGCCG AGTGCCGCAC AGCGCAGATG CAGACGTGCT CAGCCGTGGC GCCGGCGCCG 
GGCTGCCAGC TGGTGCTGCG CCAGGACTTC AACCAGTCCG GCCTCTACTG CCTCAACGTC 
TCGTTGGCCA ACGGCAACGG CTTGGCTGTG GCCAGCACCC ACGTGGCTGT CGGAGGAGCA 
TCCCCAGCCG CCAGTGGCAC CACGCTCACC GTGGGGCTGC TCTGGGCACC GCTCATGGCC 
GCTGCTCTGG GCACCGCTGC CTACACCTAC CGCCGTGTGA AGTACAGCCC GCTGCTGCCC 
ACAGCCCCCA CGGCCCCCCG GCCCCACAGC TGGCTGCCCC CCGGTGCTAC CCTGCGTTTG 
CTGCTGCGCC AGGCTTTTGG GGGGGCTCCC AGTGGAGAAA GCAGCCCCCT GCTGCGTGCC 
AACGCTGTCT AG