LOC105916449 cDNA ORF clone, Fundulus heteroclitus(Mummichog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105916449
Entrez Gene ID	105916449
Full Name	ectonucleotide pyrophosphatase/phosphodiesterase family member 2
Gene Type	protein-coding
Organism	Fundulus heteroclitus(Mummichog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021309712.1	XP_021165387.1	ectonucleotide pyrophosphatase/phosphodiesterase family member 2
XM_012850978.1	XP_012706432.1	ectonucleotide pyrophosphatase/phosphodiesterase family member 2-like

Pathways from BioSystems
Gene Ontology

The following LOC105916449 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105916449 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFu100885	XM_012850978.1 Latest version!	Fundulus heteroclitus ectonucleotide pyrophosphatase/phosphodiesterase family member 2-like (LOC105916449), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OFu128145	XM_021309712.1 Latest version!	Fundulus heteroclitus ectonucleotide pyrophosphatase/phosphodiesterase family member 2 (LOC105916449), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105916449 ( XM_012850978.1 ) cDNA ORF clone, Fundulus heteroclitus(Mummichog) -> XP_012706432.1 Fundulus heteroclitus ectonucleotide pyrophosphatase/phosphodiesterase family member 2-like (LOC105916449), mRNA.

Price & Availability↑

CloneID	OFu100885
Clone ID Related Accession (Same CDS sequence)	XM_012850978.1
Accession Version	XM_012850978.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	ectonucleotide pyrophosphatase/phosphodiesterase family member 2-like
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012224577.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGG GCAATTTGAT AGTGTTAGTG TTCTGTCTTT GGGGAACGGA TGTCAGTAGA 
GCTCACATAT TTGAAGCCAC ACGATCTGGA GAAGGAGATA ACTGGTCACC TGTCACTAAA 
CTTGTATCGG AGTGGGTCAG CACCGCGGGT TCCTGTAAGG GGAGGTGCTT TGAGCTGGAG 
GTGGCGGACC CTCCAGGATG CAGATGTGAC AATCTGTGTA AAACTTACCT CAGCTGCTGC 
TCCGACTTCG ATGCTCACTG CCTGAAAACT GCGAGGGGGT TTGAGTGTAC GCCTGATCGT 
TGCGGGGAGG AGAGGAATGA AGACCACGCC TGCCACTGCT CAGAGGATTG CCTGGAGCAA 
GGAGACTGTT GTTCGAACTA CAAATCCACC TGCAAAGATG AGAATTCGTG GTTGGAGGAC 
AGCTGTGAGG AGATCAAGAG TCCTGAATGT CCTGCAGGTT TCATTCGTCC TCCGCTCATC 
ATGCTGTCTG TTGATGGATT CAGAGCCTCC TACCTGAAGA AGGGCCCATT TGTCATCCCC 
AACTTACATA AACTCAGATC ATGTGGCGTA TCGCCCCCAT ACATGCGACC AGTCTACCCA 
ACAAAGACGT TTCCAAATTT ATACACTTTA GCCACAGGTC TGTACCCGGA GTCCCATGGA 
ATTGTGGGAA ACACCATGTA TGACCCAGTG TTCAATGCCA CCTTCAGCCT GCGCACAAGG 
GAGAAACTGA ACCACCGCTG GTGGGGTGGA CAGCCATTTC TATCATATAA AACACTTCAA 
TTCCTCAACC TGATATTTCT TTCCAGGGTG ATTCCTCTTG AAAGAAGGAT TTTGACCATT 
CTCCGATGGC TGAAGCTTCC AGATCAAGAG AGGCCGTATG TTTATGCAGT CCACTCAGAG 
CAGCCGGATA CCTTTGGTCA TCGTCTTGGA CCACTCAGCA GCGAGCTGGA CAACCCCCTG 
AGGGAAATCG ACAACATCAT TGGTCAGCTT ATGAATGGTC TCAAACAGAT GAATCTGCAC 
CGATGTGTCA ACGTCATCAT TGTGGGAGAT CACGGTATGG AAGAAACCCA CTGTGACAGG 
ACAGAGTTCC TCAGCAGCTA TCCTCTAAAC TTAGATGACA TCATGCTCAT CCCTGGCTCC 
ATTGGTAGAA TACGCCCCCG TGACCCCAAG TCAACCACAT ATGACCCAAA GGTCGTGGTT 
GGAAACCTTA CTTGTAAAAT GCCTTCTCAG CACTTTAAAC CATACCTGAA GCAGCATTTA 
CCCAAGAGAC TTCACTACGC CAACCACCGC AGGATCGAAG ATGTCCAGCT GATGATGCAG 
AGGAAGTGGC ACATTGCTAA GAAGACGCCA GAAAAACCCA GATCTCGGTG TGGTTTCTTT 
GGTGACCATG GCTTCGACAA CAGGATAACC AGCATGAGGA CGATCTTTGT CGGCCATGGA 
CCAAGCTTCA AATTTCAGAA AAGTGTGCCA GAGTTTGACA ATATAGAGCT ATACAACGTC 
ATGTGTGACC TGTTGGGGTT GAAGCCAGCT CCTAACAATG GGACACATGG CAGCCTGAAC 
GACATGCTGA AGAATCCACC GTTCCAGCCC ACCTTGCCAG AGGAAGTCAC ACCTCCGTCA 
CCTGCATCCG ACCCCGACAA CGCAATGACT CAAGATCTGG GATGCAGCTG TGAGGATGAG 
AACAGGGTGG AGGAGGTCAT TGAAACCTTC ACCCCTGCAC CCGATGGGAT TTACAGCTAC 
AACAGAACCC ATCTTCCGTT TGGTCGGCCG GCGGTGATGT TCAACACTCA GTACAGTCTA 
CTTCATCATG TGGAATTCAT CAGTGGATAC AGCAAAGAGC TGGCAATGCC ACTCTGGACA 
GCATTCACGC TGCCCCAAAA GGAGTATCTG GCTCCCGTTC CGGAGGTTTC TCCCTCTGCC 
AACTGTATCC GAGTCAACCC GAGAGTTTCG TCAGTACACA GTTTGAGCTG CACAGCCTAC 
AACAAGAACA GCCACCTGAC CTATGGCTTC CTCTTCCCCC CAGATCTGGC AACTTCTCCA 
GAGTCCAGAT ATGATTCTTC GCTCATCACC AACACTGTTC CCATGTACCC CGCTTTTAAA 
AGGATATGGA CTTACCTGCA GGGGACGCTG CTTAGACGTT ACAGTGATGA AAACAATGGT 
ATCAATGTTC TGTCCGGGCC AATCTTCGAC TATAACTATG ACGGCCTCCG AGACACCACG 
GAGCAGATTA AAGAGTTTTC AGTGGACACG CCGCCCATTC CCACCCACTT CTACGTCATT 
GTGACATGCT GCCAGGAGAT TAACCAGACG GTGGATGAGT GTGACGGGGC GCTCAAGGTG 
TTTTCCTTCA TCCTTCCTCA CCGGCCAGAC AACAGTGAAA CCTGCAATAG CGCCGAGGAA 
GAGTCCCTGT GGGTTGAAGA CATGCTGAAG CACCACACGG CCCGAGTTCG GGATGTGGAG 
ATCTTGACGG GCCTGGACCT GTACCGATCG ACCAACCTCA CCTACACCAG CACTCTGAGC 
CTCAAAACCT ACATAAACAC CTTCGAGAGT GACGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012706432.1
CDS	266..2824
Translation	MLLGNLIVLVFCLWGTDVSRAHIFEATRSGEGDNWSPVTKLVSEWVSTAGSCKGRCFELEVADPPGCRCDNLCKTYLSCCSDFDAHCLKTARGFECTPDRCGEERNEDHACHCSEDCLEQGDCCSNYKSTCKDENSWLEDSCEEIKSPECPAGFIRPPLIMLSVDGFRASYLKKGPFVIPNLHKLRSCGVSPPYMRPVYPTKTFPNLYTLATGLYPESHGIVGNTMYDPVFNATFSLRTREKLNHRWWGGQPFLSYKTLQFLNLIFLSRVIPLERRILTILRWLKLPDQERPYVYAVHSEQPDTFGHRLGPLSSELDNPLREIDNIIGQLMNGLKQMNLHRCVNVIIVGDHGMEETHCDRTEFLSSYPLNLDDIMLIPGSIGRIRPRDPKSTTYDPKVVVGNLTCKMPSQHFKPYLKQHLPKRLHYANHRRIEDVQLMMQRKWHIAKKTPEKPRSRCGFFGDHGFDNRITSMRTIFVGHGPSFKFQKSVPEFDNIELYNVMCDLLGLKPAPNNGTHGSLNDMLKNPPFQPTLPEEVTPPSPASDPDNAMTQDLGCSCEDENRVEEVIETFTPAPDGIYSYNRTHLPFGRPAVMFNTQYSLLHHVEFISGYSKELAMPLWTAFTLPQKEYLAPVPEVSPSANCIRVNPRVSSVHSLSCTAYNKNSHLTYGFLFPPDLATSPESRYDSSLITNTVPMYPAFKRIWTYLQGTLLRRYSDENNGINVLSGPIFDYNYDGLRDTTEQIKEFSVDTPPIPTHFYVIVTCCQEINQTVDECDGALKVFSFILPHRPDNSETCNSAEEESLWVEDMLKHHTARVRDVEILTGLDLYRSTNLTYTSTLSLKTYINTFESDD

Target ORF information:

RefSeq Version	XM_012850978.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus ectonucleotide pyrophosphatase/phosphodiesterase family member 2-like (LOC105916449), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012850978.1

ORF Insert Sequence:

ATGCTGCTGG GCAATTTGAT AGTGTTAGTG TTCTGTCTTT GGGGAACGGA TGTCAGTAGA 
GCTCACATAT TTGAAGCCAC ACGATCTGGA GAAGGAGATA ACTGGTCACC TGTCACTAAA 
CTTGTATCGG AGTGGGTCAG CACCGCGGGT TCCTGTAAGG GGAGGTGCTT TGAGCTGGAG 
GTGGCGGACC CTCCAGGATG CAGATGTGAC AATCTGTGTA AAACTTACCT CAGCTGCTGC 
TCCGACTTCG ATGCTCACTG CCTGAAAACT GCGAGGGGGT TTGAGTGTAC GCCTGATCGT 
TGCGGGGAGG AGAGGAATGA AGACCACGCC TGCCACTGCT CAGAGGATTG CCTGGAGCAA 
GGAGACTGTT GTTCGAACTA CAAATCCACC TGCAAAGATG AGAATTCGTG GTTGGAGGAC 
AGCTGTGAGG AGATCAAGAG TCCTGAATGT CCTGCAGGTT TCATTCGTCC TCCGCTCATC 
ATGCTGTCTG TTGATGGATT CAGAGCCTCC TACCTGAAGA AGGGCCCATT TGTCATCCCC 
AACTTACATA AACTCAGATC ATGTGGCGTA TCGCCCCCAT ACATGCGACC AGTCTACCCA 
ACAAAGACGT TTCCAAATTT ATACACTTTA GCCACAGGTC TGTACCCGGA GTCCCATGGA 
ATTGTGGGAA ACACCATGTA TGACCCAGTG TTCAATGCCA CCTTCAGCCT GCGCACAAGG 
GAGAAACTGA ACCACCGCTG GTGGGGTGGA CAGCCATTTC TATCATATAA AACACTTCAA 
TTCCTCAACC TGATATTTCT TTCCAGGGTG ATTCCTCTTG AAAGAAGGAT TTTGACCATT 
CTCCGATGGC TGAAGCTTCC AGATCAAGAG AGGCCGTATG TTTATGCAGT CCACTCAGAG 
CAGCCGGATA CCTTTGGTCA TCGTCTTGGA CCACTCAGCA GCGAGCTGGA CAACCCCCTG 
AGGGAAATCG ACAACATCAT TGGTCAGCTT ATGAATGGTC TCAAACAGAT GAATCTGCAC 
CGATGTGTCA ACGTCATCAT TGTGGGAGAT CACGGTATGG AAGAAACCCA CTGTGACAGG 
ACAGAGTTCC TCAGCAGCTA TCCTCTAAAC TTAGATGACA TCATGCTCAT CCCTGGCTCC 
ATTGGTAGAA TACGCCCCCG TGACCCCAAG TCAACCACAT ATGACCCAAA GGTCGTGGTT 
GGAAACCTTA CTTGTAAAAT GCCTTCTCAG CACTTTAAAC CATACCTGAA GCAGCATTTA 
CCCAAGAGAC TTCACTACGC CAACCACCGC AGGATCGAAG ATGTCCAGCT GATGATGCAG 
AGGAAGTGGC ACATTGCTAA GAAGACGCCA GAAAAACCCA GATCTCGGTG TGGTTTCTTT 
GGTGACCATG GCTTCGACAA CAGGATAACC AGCATGAGGA CGATCTTTGT CGGCCATGGA 
CCAAGCTTCA AATTTCAGAA AAGTGTGCCA GAGTTTGACA ATATAGAGCT ATACAACGTC 
ATGTGTGACC TGTTGGGGTT GAAGCCAGCT CCTAACAATG GGACACATGG CAGCCTGAAC 
GACATGCTGA AGAATCCACC GTTCCAGCCC ACCTTGCCAG AGGAAGTCAC ACCTCCGTCA 
CCTGCATCCG ACCCCGACAA CGCAATGACT CAAGATCTGG GATGCAGCTG TGAGGATGAG 
AACAGGGTGG AGGAGGTCAT TGAAACCTTC ACCCCTGCAC CCGATGGGAT TTACAGCTAC 
AACAGAACCC ATCTTCCGTT TGGTCGGCCG GCGGTGATGT TCAACACTCA GTACAGTCTA 
CTTCATCATG TGGAATTCAT CAGTGGATAC AGCAAAGAGC TGGCAATGCC ACTCTGGACA 
GCATTCACGC TGCCCCAAAA GGAGTATCTG GCTCCCGTTC CGGAGGTTTC TCCCTCTGCC 
AACTGTATCC GAGTCAACCC GAGAGTTTCG TCAGTACACA GTTTGAGCTG CACAGCCTAC 
AACAAGAACA GCCACCTGAC CTATGGCTTC CTCTTCCCCC CAGATCTGGC AACTTCTCCA 
GAGTCCAGAT ATGATTCTTC GCTCATCACC AACACTGTTC CCATGTACCC CGCTTTTAAA 
AGGATATGGA CTTACCTGCA GGGGACGCTG CTTAGACGTT ACAGTGATGA AAACAATGGT 
ATCAATGTTC TGTCCGGGCC AATCTTCGAC TATAACTATG ACGGCCTCCG AGACACCACG 
GAGCAGATTA AAGAGTTTTC AGTGGACACG CCGCCCATTC CCACCCACTT CTACGTCATT 
GTGACATGCT GCCAGGAGAT TAACCAGACG GTGGATGAGT GTGACGGGGC GCTCAAGGTG 
TTTTCCTTCA TCCTTCCTCA CCGGCCAGAC AACAGTGAAA CCTGCAATAG CGCCGAGGAA 
GAGTCCCTGT GGGTTGAAGA CATGCTGAAG CACCACACGG CCCGAGTTCG GGATGTGGAG 
ATCTTGACGG GCCTGGACCT GTACCGATCG ACCAACCTCA CCTACACCAG CACTCTGAGC 
CTCAAAACCT ACATAAACAC CTTCGAGAGT GACGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105916449 ( XM_021309712.1 ) cDNA ORF clone, Fundulus heteroclitus(Mummichog) -> XP_021165387.1 Fundulus heteroclitus ectonucleotide pyrophosphatase/phosphodiesterase family member 2 (LOC105916449), mRNA.

Price & Availability↑

CloneID	OFu128145
Clone ID Related Accession (Same CDS sequence)	XM_021309712.1
Accession Version	XM_021309712.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	ectonucleotide pyrophosphatase/phosphodiesterase family member 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012224577.1) and transcript sequence (GCES01030863.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 113 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCTGCTGG GCAATTTGAT AGTGTTAGTG TTCTGTCTTT GGGGAACGGA TGTCAGTAGA 
GCTCACATAT TTGAAGCCAC ACGATCTGGA GAAGGAGATA ACTGGTCACC TGTCACTAAA 
CTTGTATCGG AGTGGGTCAG CACCGCGGGT TCCTGTAAGG GGAGGTGCTT TGAGCTGGAG 
GTGGCGGACC CTCCAGGATG CAGATGTGAC AATCTGTGTA AAACTTACCT CAGCTGCTGC 
TCCGACTTCG ATGCTCACTG CCTGAAAACT GCGAGGGGGT TTGAGTGTAC GCCTGATCGT 
TGCGGGGAGG AGAGGAATGA AGACCACGCC TGCCACTGCT CAGAGGATTG CCTGGAGCAA 
GGAGACTGTT GTTCGAACTA CAAATCCACC TGCAAAGATG AGAATTCGTG GTTGGAGGAC 
AGCTGTGAGG AGATCAAGAG TCCTGAATGT CCTGCAGGTT TCATTCGTCC TCCGCTCATC 
ATGCTGTCTG TTGATGGATT CAGAGCCTCC TACCTGAAGA AGGGCCCATT TGTCATCCCC 
AACTTACATA AACTCAGATC ATGTGGCGTA TCGCCCCCAT ACATGCGACC AGTCTACCCA 
ACAAAGACGT TTCCAAATTT ATACACTTTA GCCACAGGTC TGTACCCGGA GTCCCATGGA 
ATTGTGGGAA ACACCATGTA TGACCCAGTG TTCAATGCCA CCTTCAGCCT GCGCACAAGG 
GAGAAACTGA ACCACCGCTG GTGGGGTGGA CAGCCAATCT GGATTACAGC CGAGAAACAG 
GGTCTGAACC ACCGCTGGTG GGGTGGACAG CCAATCTGGA TTACAGCCGA GAAACAGGGT 
GTAAAAGCAG GAACTTTCTT CTGGCCATGG GTGATTCCTC TTGAAAGAAG GATTTTGACC 
ATTCTCCGAT GGCTGAAGCT TCCAGATCAA GAGAGGCCGT ATGTTTATGC AGTCCACTCA 
GAGCAGCCGG ATACCTTTGG TCATCGTCTT GGACCACTCA GCAGCGAGCT GGACAACCCC 
CTGAGGGAAA TCGACAACAT CATTGGTCAG CTTATGAATG GTCTCAAACA GATGAATCTG 
CACCGATGTG TCAACGTCAT CATTGTGGGA GATCACGGTA TGGAAGAAAC CCACTGTGAC 
AGGACAGAGT TCCTCAGCAG CTATCCTCTA AACTTAGATG ACATCATGCT CATCCCTGGC 
TCCATTGGTA GAATACGCCC CCGTGACCCC AAGTCAACCA CATATGACCC AAAGGTCGTG 
GTTGGAAACC TTACTTGTAA AATGCCTTCT CAGCACTTTA AACCATACCT GAAGCAGCAT 
TTACCCAAGA GACTTCACTA CGCCAACCAC CGCAGGATCG AAGATGTCCA GCTGATGATG 
CAGAGGAAGT GGCACATTGC TAAGAAGACG CCAGAAAAAC CCAGATCTCG GTGTGGTTTC 
TTTGGTGACC ATGGCTTCGA CAACAGGATA ACCAGCATGA GGACGATCTT TGTCGGCCAT 
GGACCAAGCT TCAAATTTCA GAAAAGTGTG CCAGAGTTTG ACAATATAGA GCTATACAAC 
GTCATGTGTG ACCTGTTGGG GTTGAAGCCA GCTCCTAACA ATGGGACACA TGGCAGCCTG 
AACGACATGC TGAAGAATCC ACCGTTCCAG CCCACCTTGC CAGAGGAAGT CACACCTCCG 
TCACCTGCAT CCGACCCCGA CAACGCAATG ACTCAAGATC TGGGATGCAG CTGTGAGGAT 
GAGAACAGGG TGGAGGAGGT CATTGAAACC TTCACCCCTG CACCCGATGG GATTTACAGC 
TACAACAGAA CCCATCTTCC GTTTGGTCGG CCGGCGGTGA TGTTCAACAC TCAGTACAGT 
CTACTTCATC ATGTGGAATT CATCAGTGGA TACAGCAAAG AGCTGGCAAT GCCACTCTGG 
ACAGCATTCA CGCTGCCCCA AAAGGAGTAT CTGGCTCCCG TTCCGGAGGT TTCTCCCTCT 
GCCAACTGTA TCCGAGTCAA CCCGAGAGTT TCGTCAGTAC ACAGTTTGAG CTGCACAGCC 
TACAACAAGA ACAGCCACCT GACCTATGGC TTCCTCTTCC CCCCAGATCT GGCAACTTCT 
CCAGAGTCCA GATATGATTC TTCGCTCATC ACCAACACTG TTCCCATGTA CCCCGCTTTT 
AAAAGGATAT GGACTTACCT GCAGGGGACG CTGCTTAGAC GTTACAGTGA TGAAAACAAT 
GGTATCAATG TTCTGTCCGG GCCAATCTTC GACTATAACT ATGACGGCCT CCGAGACACC 
ACGGAGCAGA TTAAAGAGTT TTCAGTGGAC ACGCCGCCCA TTCCCACCCA CTTCTACGTC 
ATTGTGACAT GCTGCCAGGA GATTAACCAG ACGGTGGATG AGTGTGACGG GGCGCTCAAG 
GTGTTTTCCT TCATCCTTCC TCACCGGCCA GACAACAGTG AAACCTGCAA TAGCGCCGAG 
GAAGAGTCCC TGTGGGTTGA AGACATGCTG AAGCACCACA CGGCCCGAGT TCGGGATGTG 
GAGATCTTGA CGGGCCTGGA CCTGTACCGA TCGACCAACC TCACCTACAC CAGCACTCTG 
AGCCTCAAAA CCTACATAAA CACCTTCGAG AGTGACGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021165387.1
CDS	190..2811
Translation	MLLGNLIVLVFCLWGTDVSRAHIFEATRSGEGDNWSPVTKLVSEWVSTAGSCKGRCFELEVADPPGCRCDNLCKTYLSCCSDFDAHCLKTARGFECTPDRCGEERNEDHACHCSEDCLEQGDCCSNYKSTCKDENSWLEDSCEEIKSPECPAGFIRPPLIMLSVDGFRASYLKKGPFVIPNLHKLRSCGVSPPYMRPVYPTKTFPNLYTLATGLYPESHGIVGNTMYDPVFNATFSLRTREKLNHRWWGGQPIWITAEKQGLNHRWWGGQPIWITAEKQGVKAGTFFWPWVIPLERRILTILRWLKLPDQERPYVYAVHSEQPDTFGHRLGPLSSELDNPLREIDNIIGQLMNGLKQMNLHRCVNVIIVGDHGMEETHCDRTEFLSSYPLNLDDIMLIPGSIGRIRPRDPKSTTYDPKVVVGNLTCKMPSQHFKPYLKQHLPKRLHYANHRRIEDVQLMMQRKWHIAKKTPEKPRSRCGFFGDHGFDNRITSMRTIFVGHGPSFKFQKSVPEFDNIELYNVMCDLLGLKPAPNNGTHGSLNDMLKNPPFQPTLPEEVTPPSPASDPDNAMTQDLGCSCEDENRVEEVIETFTPAPDGIYSYNRTHLPFGRPAVMFNTQYSLLHHVEFISGYSKELAMPLWTAFTLPQKEYLAPVPEVSPSANCIRVNPRVSSVHSLSCTAYNKNSHLTYGFLFPPDLATSPESRYDSSLITNTVPMYPAFKRIWTYLQGTLLRRYSDENNGINVLSGPIFDYNYDGLRDTTEQIKEFSVDTPPIPTHFYVIVTCCQEINQTVDECDGALKVFSFILPHRPDNSETCNSAEEESLWVEDMLKHHTARVRDVEILTGLDLYRSTNLTYTSTLSLKTYINTFESDD

Target ORF information:

RefSeq Version	XM_021309712.1
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus ectonucleotide pyrophosphatase/phosphodiesterase family member 2 (LOC105916449), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021309712.1

ORF Insert Sequence:

ATGCTGCTGG GCAATTTGAT AGTGTTAGTG TTCTGTCTTT GGGGAACGGA TGTCAGTAGA 
GCTCACATAT TTGAAGCCAC ACGATCTGGA GAAGGAGATA ACTGGTCACC TGTCACTAAA 
CTTGTATCGG AGTGGGTCAG CACCGCGGGT TCCTGTAAGG GGAGGTGCTT TGAGCTGGAG 
GTGGCGGACC CTCCAGGATG CAGATGTGAC AATCTGTGTA AAACTTACCT CAGCTGCTGC 
TCCGACTTCG ATGCTCACTG CCTGAAAACT GCGAGGGGGT TTGAGTGTAC GCCTGATCGT 
TGCGGGGAGG AGAGGAATGA AGACCACGCC TGCCACTGCT CAGAGGATTG CCTGGAGCAA 
GGAGACTGTT GTTCGAACTA CAAATCCACC TGCAAAGATG AGAATTCGTG GTTGGAGGAC 
AGCTGTGAGG AGATCAAGAG TCCTGAATGT CCTGCAGGTT TCATTCGTCC TCCGCTCATC 
ATGCTGTCTG TTGATGGATT CAGAGCCTCC TACCTGAAGA AGGGCCCATT TGTCATCCCC 
AACTTACATA AACTCAGATC ATGTGGCGTA TCGCCCCCAT ACATGCGACC AGTCTACCCA 
ACAAAGACGT TTCCAAATTT ATACACTTTA GCCACAGGTC TGTACCCGGA GTCCCATGGA 
ATTGTGGGAA ACACCATGTA TGACCCAGTG TTCAATGCCA CCTTCAGCCT GCGCACAAGG 
GAGAAACTGA ACCACCGCTG GTGGGGTGGA CAGCCAATCT GGATTACAGC CGAGAAACAG 
GGTCTGAACC ACCGCTGGTG GGGTGGACAG CCAATCTGGA TTACAGCCGA GAAACAGGGT 
GTAAAAGCAG GAACTTTCTT CTGGCCATGG GTGATTCCTC TTGAAAGAAG GATTTTGACC 
ATTCTCCGAT GGCTGAAGCT TCCAGATCAA GAGAGGCCGT ATGTTTATGC AGTCCACTCA 
GAGCAGCCGG ATACCTTTGG TCATCGTCTT GGACCACTCA GCAGCGAGCT GGACAACCCC 
CTGAGGGAAA TCGACAACAT CATTGGTCAG CTTATGAATG GTCTCAAACA GATGAATCTG 
CACCGATGTG TCAACGTCAT CATTGTGGGA GATCACGGTA TGGAAGAAAC CCACTGTGAC 
AGGACAGAGT TCCTCAGCAG CTATCCTCTA AACTTAGATG ACATCATGCT CATCCCTGGC 
TCCATTGGTA GAATACGCCC CCGTGACCCC AAGTCAACCA CATATGACCC AAAGGTCGTG 
GTTGGAAACC TTACTTGTAA AATGCCTTCT CAGCACTTTA AACCATACCT GAAGCAGCAT 
TTACCCAAGA GACTTCACTA CGCCAACCAC CGCAGGATCG AAGATGTCCA GCTGATGATG 
CAGAGGAAGT GGCACATTGC TAAGAAGACG CCAGAAAAAC CCAGATCTCG GTGTGGTTTC 
TTTGGTGACC ATGGCTTCGA CAACAGGATA ACCAGCATGA GGACGATCTT TGTCGGCCAT 
GGACCAAGCT TCAAATTTCA GAAAAGTGTG CCAGAGTTTG ACAATATAGA GCTATACAAC 
GTCATGTGTG ACCTGTTGGG GTTGAAGCCA GCTCCTAACA ATGGGACACA TGGCAGCCTG 
AACGACATGC TGAAGAATCC ACCGTTCCAG CCCACCTTGC CAGAGGAAGT CACACCTCCG 
TCACCTGCAT CCGACCCCGA CAACGCAATG ACTCAAGATC TGGGATGCAG CTGTGAGGAT 
GAGAACAGGG TGGAGGAGGT CATTGAAACC TTCACCCCTG CACCCGATGG GATTTACAGC 
TACAACAGAA CCCATCTTCC GTTTGGTCGG CCGGCGGTGA TGTTCAACAC TCAGTACAGT 
CTACTTCATC ATGTGGAATT CATCAGTGGA TACAGCAAAG AGCTGGCAAT GCCACTCTGG 
ACAGCATTCA CGCTGCCCCA AAAGGAGTAT CTGGCTCCCG TTCCGGAGGT TTCTCCCTCT 
GCCAACTGTA TCCGAGTCAA CCCGAGAGTT TCGTCAGTAC ACAGTTTGAG CTGCACAGCC 
TACAACAAGA ACAGCCACCT GACCTATGGC TTCCTCTTCC CCCCAGATCT GGCAACTTCT 
CCAGAGTCCA GATATGATTC TTCGCTCATC ACCAACACTG TTCCCATGTA CCCCGCTTTT 
AAAAGGATAT GGACTTACCT GCAGGGGACG CTGCTTAGAC GTTACAGTGA TGAAAACAAT 
GGTATCAATG TTCTGTCCGG GCCAATCTTC GACTATAACT ATGACGGCCT CCGAGACACC 
ACGGAGCAGA TTAAAGAGTT TTCAGTGGAC ACGCCGCCCA TTCCCACCCA CTTCTACGTC 
ATTGTGACAT GCTGCCAGGA GATTAACCAG ACGGTGGATG AGTGTGACGG GGCGCTCAAG 
GTGTTTTCCT TCATCCTTCC TCACCGGCCA GACAACAGTG AAACCTGCAA TAGCGCCGAG 
GAAGAGTCCC TGTGGGTTGA AGACATGCTG AAGCACCACA CGGCCCGAGT TCGGGATGTG 
GAGATCTTGA CGGGCCTGGA CCTGTACCGA TCGACCAACC TCACCTACAC CAGCACTCTG 
AGCCTCAAAA CCTACATAAA CACCTTCGAG AGTGACGACT AG