AYL99_04526 cDNA ORF clone, Fonsecaea erecta

Gene
Clones
gRNAs

Summary

Gene Symbol	AYL99_04526
Entrez Gene ID	30008695
Full Name	sphingomyelin phosphodiesterase
Gene Type	protein-coding
Organism	Fonsecaea erecta

mRNA and Protein(s)

mRNA	Protein	Name
XM_018836040.1	XP_018695690.1	sphingomyelin phosphodiesterase

Pathways from BioSystems
Gene Ontology

The following AYL99_04526 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AYL99_04526 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFo16868	XM_018836040.1 Latest version!	Fonsecaea erecta sphingomyelin phosphodiesterase (AYL99_04526), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AYL99_04526 ( XM_018836040.1 ) cDNA ORF clone, Fonsecaea erecta -> XP_018695690.1 Fonsecaea erecta sphingomyelin phosphodiesterase (AYL99_04526), partial mRNA.

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CloneID	OFo16868
Clone ID Related Accession (Same CDS sequence)	XM_018836040.1
Accession Version	XM_018836040.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-27
Organism	Fonsecaea erecta
Product	sphingomyelin phosphodiesterase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_017387246). COMPLETENESS: incomplete on both ends.

ATGATGTTGA CCTGTGTCTG CGGCTTGCTC GTGCTGGCCT CCACTTCTCT CGTTCATGGA 
GCGATTAACT CGACCGCCCA AGCCTTGAAT ACGACCGACA CAAATCTGAT TCCCAATGGA 
AATGGACCTC TTCTCTATTA CAATGGCTCT GGGCCAGTTC CCTCGTACGA CCTTGTCTCG 
CCGGTCCCAC CACCAATCAC GCCCATCAAT AGCAGCTCCG CCTTGGAGGA CATGTTCTTT 
ACCGAGTTGT ATGCCATTGC GAATACCACA ACATTTTCCA ACAACTGTTC TCAGTGCGTT 
GCGGGTGCCG AGTTGATGCA TATAGCAGCA ATCACATTGC CAGTCGACAA TTTCGTCAAT 
ATCTTGATCA GAATATGCGA AGCCGTCCCA ACAGTGCAGA ACTCCGTTTT CGCCGATAGT 
TGTGCGGCAG AGTACGGTAG CGTTCAAAAC ACTTCCATTC CATACTCGTC TGGAGGAGGT 
GCTACAGGAG CCTACTACGC TCAGCTCTTT TCCAAAATGA GCATTGCAAC TGGTGACATG 
ACATACTACT GCTACTTCAA CTGGAACGTG TGTCCAGAAC CTCCGACAAT CGGCATTGAA 
GAGAGCCTGT ACTTCAGCCC CAAGCCAGCC AATGCAAGCA CTGCGCCAGC ATCATCAGGT 
AAATCCATCA ATGTCCTACA TACTTCGGAC TGGCATCTGG ATCCACGCTA CGATATTGGG 
AGCGAGGCAA ATTGTTCTCA ATATCTCTGC TGCCGTCCGT ATGCCACCAA CGATGATTTG 
TCGACGACCT CCGCGAATGC CAGTGTCCCA GCCTCACGCT TCGGCTATCT CTACTGTGAC 
TCCCCTCCTG ACCTAGCGTT GAGTTCTTTC ACGAGCATGC ATCAATTCTT CGATTTGAGC 
AATGTCTCAT TCGCAATCTT TACTGGTGAC ATTTTGTCAC ACGATAACGA TGACCAGATA 
TCTCGCGAAT ATGCCGAGTA TGAAGAAACA ATCACCTATC AAACCTTCAA GGCTCAACTG 
GGCGACATTC CAGTGTATGC TACGCTTGGA AATCACGATT CCATTCCTGA AGCGTGGAAT 
ACGCAAAATA GTCTCAACCC GGGAGGCTCA ACTGCTGCTT CGGCTAATGC ATTTGGGTGG 
AACTATGAGC TCCTTTCCTC CCTCTGGGGC AACTCTGGCT GGATCAACAG CACTGAGCAA 
GACTACGCTT CCACGCACTA CGGTGCATAC GCACACACCA CTGCTCAAGG TCTTCGTATC 
ATCTCGATCA ACACCGACTT CTGGTACGTC GACAACATCT TCAACTATTG GAATGTGACA 
AATCCCGACA CCTCGGGCAT CTTGGCATGG CTGGCATCTG AGCTTGCTGC GTGCGAGGCC 
CGAGGCCAAC GTGCGTGGAT CATTGGGCAT GTCCCTTCGG GATACGACGG GAGCAACGCT 
GTCCTCAATC CGTCAGCACT CTTCTACAGC ATTGTCGTGC GCTTTTCCCC TGCGACGATT 
GCCGGTGTCT TCTTCGGCCA TACCCACGAA GACCAAATCC AAATCTTCTA TGATTTCCTT 
CCAAACAGCA CGTATACTAT GGACGGCAAG ACGTATCGGA ATACCACTAT GGTCGACTAC 
TCTAAGCCGT TGACAGTTGG TTACATCGGA CCTTCCATCA CGCCTCTGAC TGGAAACAAT 
GCTGGTTATC AACTCTATCA GGTCGATGCG TCGACGTTCT CCATCACGGG TATTCAAACT 
TACTTTGCCA ACGTTAGCGA GGCGAATAGC TGGACCACAC CCGAGTGGCG TTTCGAATAT 
GACGCCAGGG CCACCTATGC AGTCGCAGCC GCTCATAACA GCTCTTCTGG GCATTGGCCG 
ACGAGCGCTC CTTTGAACGC GACCTTTTGG GACGGCGTCA CAAAAAGCAT GTTGACCAAT 
CAGACTTTGG TGGAGTTGTA TAATCTACTT GAGACCAAGA GTAGCGTAGT GACCGAGAAA 
TGCTCTACGG CTGCATGTGC GAAGCAAAAG GTTTGCTACA TTCGAAGCGG AAGTGCGACC 
TTGGGCTTGG CTTGTGGTGA TCGCAGTGGA CCTTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018695690.1
CDS	1..2079
Translation	MMLTCVCGLLVLASTSLVHGAINSTAQALNTTDTNLIPNGNGPLLYYNGSGPVPSYDLVSPVPPPITPINSSSALEDMFFTELYAIANTTTFSNNCSQCVAGAELMHIAAITLPVDNFVNILIRICEAVPTVQNSVFADSCAAEYGSVQNTSIPYSSGGGATGAYYAQLFSKMSIATGDMTYYCYFNWNVCPEPPTIGIEESLYFSPKPANASTAPASSGKSINVLHTSDWHLDPRYDIGSEANCSQYLCCRPYATNDDLSTTSANASVPASRFGYLYCDSPPDLALSSFTSMHQFFDLSNVSFAIFTGDILSHDNDDQISREYAEYEETITYQTFKAQLGDIPVYATLGNHDSIPEAWNTQNSLNPGGSTAASANAFGWNYELLSSLWGNSGWINSTEQDYASTHYGAYAHTTAQGLRIISINTDFWYVDNIFNYWNVTNPDTSGILAWLASELAACEARGQRAWIIGHVPSGYDGSNAVLNPSALFYSIVVRFSPATIAGVFFGHTHEDQIQIFYDFLPNSTYTMDGKTYRNTTMVDYSKPLTVGYIGPSITPLTGNNAGYQLYQVDASTFSITGIQTYFANVSEANSWTTPEWRFEYDARATYAVAAAHNSSSGHWPTSAPLNATFWDGVTKSMLTNQTLVELYNLLETKSSVVTEKCSTAACAKQKVCYIRSGSATLGLACGDRSGPF

Target ORF information:

RefSeq Version	XM_018836040.1
Organism	Fonsecaea erecta
Definition	Fonsecaea erecta sphingomyelin phosphodiesterase (AYL99_04526), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018836040.1

ORF Insert Sequence:

ATGATGTTGA CCTGTGTCTG CGGCTTGCTC GTGCTGGCCT CCACTTCTCT CGTTCATGGA 
GCGATTAACT CGACCGCCCA AGCCTTGAAT ACGACCGACA CAAATCTGAT TCCCAATGGA 
AATGGACCTC TTCTCTATTA CAATGGCTCT GGGCCAGTTC CCTCGTACGA CCTTGTCTCG 
CCGGTCCCAC CACCAATCAC GCCCATCAAT AGCAGCTCCG CCTTGGAGGA CATGTTCTTT 
ACCGAGTTGT ATGCCATTGC GAATACCACA ACATTTTCCA ACAACTGTTC TCAGTGCGTT 
GCGGGTGCCG AGTTGATGCA TATAGCAGCA ATCACATTGC CAGTCGACAA TTTCGTCAAT 
ATCTTGATCA GAATATGCGA AGCCGTCCCA ACAGTGCAGA ACTCCGTTTT CGCCGATAGT 
TGTGCGGCAG AGTACGGTAG CGTTCAAAAC ACTTCCATTC CATACTCGTC TGGAGGAGGT 
GCTACAGGAG CCTACTACGC TCAGCTCTTT TCCAAAATGA GCATTGCAAC TGGTGACATG 
ACATACTACT GCTACTTCAA CTGGAACGTG TGTCCAGAAC CTCCGACAAT CGGCATTGAA 
GAGAGCCTGT ACTTCAGCCC CAAGCCAGCC AATGCAAGCA CTGCGCCAGC ATCATCAGGT 
AAATCCATCA ATGTCCTACA TACTTCGGAC TGGCATCTGG ATCCACGCTA CGATATTGGG 
AGCGAGGCAA ATTGTTCTCA ATATCTCTGC TGCCGTCCGT ATGCCACCAA CGATGATTTG 
TCGACGACCT CCGCGAATGC CAGTGTCCCA GCCTCACGCT TCGGCTATCT CTACTGTGAC 
TCCCCTCCTG ACCTAGCGTT GAGTTCTTTC ACGAGCATGC ATCAATTCTT CGATTTGAGC 
AATGTCTCAT TCGCAATCTT TACTGGTGAC ATTTTGTCAC ACGATAACGA TGACCAGATA 
TCTCGCGAAT ATGCCGAGTA TGAAGAAACA ATCACCTATC AAACCTTCAA GGCTCAACTG 
GGCGACATTC CAGTGTATGC TACGCTTGGA AATCACGATT CCATTCCTGA AGCGTGGAAT 
ACGCAAAATA GTCTCAACCC GGGAGGCTCA ACTGCTGCTT CGGCTAATGC ATTTGGGTGG 
AACTATGAGC TCCTTTCCTC CCTCTGGGGC AACTCTGGCT GGATCAACAG CACTGAGCAA 
GACTACGCTT CCACGCACTA CGGTGCATAC GCACACACCA CTGCTCAAGG TCTTCGTATC 
ATCTCGATCA ACACCGACTT CTGGTACGTC GACAACATCT TCAACTATTG GAATGTGACA 
AATCCCGACA CCTCGGGCAT CTTGGCATGG CTGGCATCTG AGCTTGCTGC GTGCGAGGCC 
CGAGGCCAAC GTGCGTGGAT CATTGGGCAT GTCCCTTCGG GATACGACGG GAGCAACGCT 
GTCCTCAATC CGTCAGCACT CTTCTACAGC ATTGTCGTGC GCTTTTCCCC TGCGACGATT 
GCCGGTGTCT TCTTCGGCCA TACCCACGAA GACCAAATCC AAATCTTCTA TGATTTCCTT 
CCAAACAGCA CGTATACTAT GGACGGCAAG ACGTATCGGA ATACCACTAT GGTCGACTAC 
TCTAAGCCGT TGACAGTTGG TTACATCGGA CCTTCCATCA CGCCTCTGAC TGGAAACAAT 
GCTGGTTATC AACTCTATCA GGTCGATGCG TCGACGTTCT CCATCACGGG TATTCAAACT 
TACTTTGCCA ACGTTAGCGA GGCGAATAGC TGGACCACAC CCGAGTGGCG TTTCGAATAT 
GACGCCAGGG CCACCTATGC AGTCGCAGCC GCTCATAACA GCTCTTCTGG GCATTGGCCG 
ACGAGCGCTC CTTTGAACGC GACCTTTTGG GACGGCGTCA CAAAAAGCAT GTTGACCAAT 
CAGACTTTGG TGGAGTTGTA TAATCTACTT GAGACCAAGA GTAGCGTAGT GACCGAGAAA 
TGCTCTACGG CTGCATGTGC GAAGCAAAAG GTTTGCTACA TTCGAAGCGG AAGTGCGACC 
TTGGGCTTGG CTTGTGGTGA TCGCAGTGGA CCTTTCTAG