ATP2A2 cDNA ORF clone, Ficedula albicollis(collared flycatcher)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATP2A2
Entrez Gene ID	101816264
Full Name	ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2
Gene Type	protein-coding
Organism	Ficedula albicollis(collared flycatcher)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005055123.2	XP_005055180.1	sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform X1
XM_005055124.2	XP_005055181.1	sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform X2

Pathways from BioSystems
Gene Ontology

The following ATP2A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP2A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFi05422	XM_005055124.2 Latest version!	Ficedula albicollis ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 (ATP2A2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OFi05421	XM_005055123.2 Latest version!	Ficedula albicollis ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 (ATP2A2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATP2A2 ( XM_005055124.2 ) cDNA ORF clone, Ficedula albicollis(collared flycatcher) -> XP_005055181.1 Ficedula albicollis ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 (ATP2A2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OFi05422
Clone ID Related Accession (Same CDS sequence)	XM_005055124.2
Accession Version	XM_005055124.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-19
Organism	Ficedula albicollis(collared flycatcher)
Product	sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_021687.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 20, 2016 this sequence version replaced XM_005055124.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ficedula albicollis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACG CGCACACAAA GACTGTGGAG GAAGTTTTGG CTTATTTCGG CGTCAACGAG 
AGCACCGGGC TCAGCCTCGA GCAAGTGAAG AAGCTGAAGG AGAAATGGGG CTCCAACGAG 
CTGCCGGCAG AGGAAGGAAA AACCTTACTT GAGCTTGTGA TTGAACAATT TGAAGACTTA 
CTAGTTAGAA TTTTGTTGCT GGCTGCTTGC ATATCTTTTG TCCTGGCCTG GTTTGAAGAA 
GGTGAAGAAA CAATCACAGC ATTTGTAGAG CCTTTTGTAA TCTTACTTAT ATTAGTAGCC 
AATGCTATTG TGGGAGTGTG GCAGGAAAGA AATGCTGAAA ATGCTATCGA AGCTCTTAAA 
GAATATGAGC CAGAGATGGG CAAAGTGTAT CGACAGGACA GAAAAAGTGT CCAGAGGATT 
AAAGCAAGAG ACATTGTCCC GGGTGATATC GTGGAAGTGG CAGTTGGAGA CAAGGTTCCT 
GCTGATATAA GAATTACTTC TATCAAATCT ACAACTCTAA GGGTAGACCA GTCAATCCTT 
ACAGGTGAAT CTGTGTCTGT TATTAAGCAC ACAGACCCTG TGCCTGATCC TCGTGCTGTA 
AATCAGGACA AGAAGAACAT GCTCTTTTCT GGTACAAACA TTGCTGCTGG TAAGGCCATG 
GGGGTGGTTA TTGCAACAGG GGTCAACACT GAAATCGGGA AGATCCGTGA CGAGATGGTG 
GCGACGGAGC AGGAACGAAC GCCCCTGCAG CAGAAGCTGG ACGAGTTTGG GGAGCAGCTG 
TCCAAGGTCA TCTCGCTGAT CTGCATCGCC GTGTGGATCA TCAACATCGG CCACTTCAAC 
GACCCCGTGC ACGGCGGCTC CTGGATCCGC GGCGCCATCT ACTACTTCAA AATCGCCGTG 
GCGCTGGCGG TGGCCGCCAT CCCCGAGGGG CTGCCCGCCG TCATCACCAC CTGCCTGGCG 
CTGGGCACGC GCCGCATGGC CAAGAAGAAC GCCATCGTCA GGAGCCTGCC CTCCGTGGAG 
ACCCTGGGCT GCACCTCCGT CATCTGCTCC GACAAGACCG GCACCCTCAC CACCAACCAG 
ATGTCCGTGT GCAGGATGTT TATCCTGGAC AGAGTAGAAG GAGACAGCTG CTCTCTGAAT 
GAATTTACTG TAACTGGTTC AACATATGCT CCCATGGGTG AAGTGCATAA AGATGACAAA 
CTCATTAAAT GCAGCCAGTA TGATGGTCTT GTGGAACTTG CAACGATCTG TGCACTCTGC 
AATGATTCCT CTTTGGATTA CAATGAAGCT AAGGGAGTTT ATGAAAAGGT TGGTGAAGCC 
ACAGAGACGG CGCTCACGTG TCTGGTTGAG AAGATGAACG TGTTTGACAC AGACCTGAAG 
GGACTTTCTA GAATCGAACG TGCCAACGCC TGCAACTCGG TGATAAAACA ACTCATGAAG 
AAAGAATTCA CTCTGGAATT CTCAAGAGAC AGGAAGTCGA TGTCTGTCTA TTGTACTCCG 
AACAAACCGA GCCGCACGTC CATGAGCAAG ATGTTTGTTA AGGGTGCTCC TGAAGGTGTG 
ATTGACAGAT GTACACATGT CCGTGTTGGA AATGCTAAAA TACCTTTAAC CCCAGGAATT 
AAACAGAAAA TAATGTCTGT CATTAGAGAA TGGGGAACTG GCAGAGACAC ATTGCGCTGC 
TTGGCTCTGG CAACCCATGA CAACCCTCCC AAAAAAGAGG AAATGAATCT GGAGGACTCC 
TCAAATTTCA TTAACTATGA GACCAACCTG ACATTTGTGG GCTGCGTGGG AATGCTGGAT 
CCCCCGAGGA TTGAAGTAGC TTCATCCATA AAGCTGTGCA GACAGGCTGG CATCAGAGTT 
ATTATGATCA CTGGTGACAA CAAAGGCACG GCTGTGGCCA TTTGCCGCCG CATCGGGATC 
TTTGTGGAGG ATGAGGACGT GTCCACCAAA GCCTTCACGG GCCGGGAGTT CGATGAGCTG 
TCCCTGGCTG CACAGAGAGA TGCCTGTCAC CACGCCCGCT GCTTTGCCCG GGTGGAGCCT 
TCCCACAAAT CCAAAATCGT GGAGTTTCTT CAGTCTTTCG ATGAGATCAC AGCCATGACT 
GGTGATGGTG TCAATGATGC CCCTGCACTG AAGAAAGCAG AGATTGGAAT TGCCATGGGT 
TCTGGTACTG CAGTGGCCAA AACTGCTTCT GAAATGGTTT TAGCAGATGA CAACTTCTCC 
ACGATTGTGG CTGCTGTGGA AGAAGGACGT GCAATTTATA ACAACATGAA ACAGTTCATC 
CGATACCTGA TCTCCTCCAA TGTTGGGGAA GTTGTTTGTA TCTTCTTGAC TGCTGCCCTG 
GGTTTTCCTG AAGCTCTGAT TCCTGTTCAG CTGCTGTGGG TAAACCTTGT GACGGATGGG 
CTCCCTGCCA CTGCCCTGGG CTTTAACCCT CCTGACCTCG ATATCATGAA CAAGCCTCCG 
CGCAACCCCA AAGAGCCCCT GATCAGTGGC TGGCTCTTCT TCCGGTACCT GGCTATTGGA 
TGTTATGTTG GTGCTGCCAC AGTGGGTGCT GCTGCTTGGT GGTTTATTGC TGCTGATGGT 
GGTCCAAAAG TTTCCTTTTA CCAGCTGAGC CATTTTCTGC AGTGCAAGGA GGACAACCCA 
GATTTCTATG GGGTGGACTG TGTGGTGTTC GAGTCCCCGT ACCCGATGAC AATGGCTCTG 
TCTGTGCTTG TCACCATTGA GATGTGCAAT GCTCTCAACA GCCTGTCAGA AAACCAGTCC 
CTGATGAGGA TGCCCCCGTG GGAGAACATC TGGCTGGTGG GGGCCATTTG CTTGTCCATG 
TCCCTCCACT TCCTTATCCT TTATGTGGAA CCACTGCCAA TTATCTTCCA GATCACACCT 
CTGAACGTGA CGCAGTGGCT GATGGTGTTG AAAATCTCCC TCCCTGTCAT TCTGCTGGAT 
GAAACACTTA AATATGTGGC CCGTAACTAC CTGGAACCTG CAATACTGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005055181.1
CDS	218..3211
Translation	MENAHTKTVEEVLAYFGVNESTGLSLEQVKKLKEKWGSNELPAEEGKTLLELVIEQFEDLLVRILLLAACISFVLAWFEEGEETITAFVEPFVILLILVANAIVGVWQERNAENAIEALKEYEPEMGKVYRQDRKSVQRIKARDIVPGDIVEVAVGDKVPADIRITSIKSTTLRVDQSILTGESVSVIKHTDPVPDPRAVNQDKKNMLFSGTNIAAGKAMGVVIATGVNTEIGKIRDEMVATEQERTPLQQKLDEFGEQLSKVISLICIAVWIINIGHFNDPVHGGSWIRGAIYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFILDRVEGDSCSLNEFTVTGSTYAPMGEVHKDDKLIKCSQYDGLVELATICALCNDSSLDYNEAKGVYEKVGEATETALTCLVEKMNVFDTDLKGLSRIERANACNSVIKQLMKKEFTLEFSRDRKSMSVYCTPNKPSRTSMSKMFVKGAPEGVIDRCTHVRVGNAKIPLTPGIKQKIMSVIREWGTGRDTLRCLALATHDNPPKKEEMNLEDSSNFINYETNLTFVGCVGMLDPPRIEVASSIKLCRQAGIRVIMITGDNKGTAVAICRRIGIFVEDEDVSTKAFTGREFDELSLAAQRDACHHARCFARVEPSHKSKIVEFLQSFDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGFPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMNKPPRNPKEPLISGWLFFRYLAIGCYVGAATVGAAAWWFIAADGGPKVSFYQLSHFLQCKEDNPDFYGVDCVVFESPYPMTMALSVLVTIEMCNALNSLSENQSLMRMPPWENIWLVGAICLSMSLHFLILYVEPLPIIFQITPLNVTQWLMVLKISLPVILLDETLKYVARNYLEPAILE

Target ORF information:

RefSeq Version	XM_005055124.2
Organism	Ficedula albicollis(collared flycatcher)
Definition	Ficedula albicollis ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 (ATP2A2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005055124.2

ORF Insert Sequence:

ATGGAGAACG CGCACACAAA GACTGTGGAG GAAGTTTTGG CTTATTTCGG CGTCAACGAG 
AGCACCGGGC TCAGCCTCGA GCAAGTGAAG AAGCTGAAGG AGAAATGGGG CTCCAACGAG 
CTGCCGGCAG AGGAAGGAAA AACCTTACTT GAGCTTGTGA TTGAACAATT TGAAGACTTA 
CTAGTTAGAA TTTTGTTGCT GGCTGCTTGC ATATCTTTTG TCCTGGCCTG GTTTGAAGAA 
GGTGAAGAAA CAATCACAGC ATTTGTAGAG CCTTTTGTAA TCTTACTTAT ATTAGTAGCC 
AATGCTATTG TGGGAGTGTG GCAGGAAAGA AATGCTGAAA ATGCTATCGA AGCTCTTAAA 
GAATATGAGC CAGAGATGGG CAAAGTGTAT CGACAGGACA GAAAAAGTGT CCAGAGGATT 
AAAGCAAGAG ACATTGTCCC GGGTGATATC GTGGAAGTGG CAGTTGGAGA CAAGGTTCCT 
GCTGATATAA GAATTACTTC TATCAAATCT ACAACTCTAA GGGTAGACCA GTCAATCCTT 
ACAGGTGAAT CTGTGTCTGT TATTAAGCAC ACAGACCCTG TGCCTGATCC TCGTGCTGTA 
AATCAGGACA AGAAGAACAT GCTCTTTTCT GGTACAAACA TTGCTGCTGG TAAGGCCATG 
GGGGTGGTTA TTGCAACAGG GGTCAACACT GAAATCGGGA AGATCCGTGA CGAGATGGTG 
GCGACGGAGC AGGAACGAAC GCCCCTGCAG CAGAAGCTGG ACGAGTTTGG GGAGCAGCTG 
TCCAAGGTCA TCTCGCTGAT CTGCATCGCC GTGTGGATCA TCAACATCGG CCACTTCAAC 
GACCCCGTGC ACGGCGGCTC CTGGATCCGC GGCGCCATCT ACTACTTCAA AATCGCCGTG 
GCGCTGGCGG TGGCCGCCAT CCCCGAGGGG CTGCCCGCCG TCATCACCAC CTGCCTGGCG 
CTGGGCACGC GCCGCATGGC CAAGAAGAAC GCCATCGTCA GGAGCCTGCC CTCCGTGGAG 
ACCCTGGGCT GCACCTCCGT CATCTGCTCC GACAAGACCG GCACCCTCAC CACCAACCAG 
ATGTCCGTGT GCAGGATGTT TATCCTGGAC AGAGTAGAAG GAGACAGCTG CTCTCTGAAT 
GAATTTACTG TAACTGGTTC AACATATGCT CCCATGGGTG AAGTGCATAA AGATGACAAA 
CTCATTAAAT GCAGCCAGTA TGATGGTCTT GTGGAACTTG CAACGATCTG TGCACTCTGC 
AATGATTCCT CTTTGGATTA CAATGAAGCT AAGGGAGTTT ATGAAAAGGT TGGTGAAGCC 
ACAGAGACGG CGCTCACGTG TCTGGTTGAG AAGATGAACG TGTTTGACAC AGACCTGAAG 
GGACTTTCTA GAATCGAACG TGCCAACGCC TGCAACTCGG TGATAAAACA ACTCATGAAG 
AAAGAATTCA CTCTGGAATT CTCAAGAGAC AGGAAGTCGA TGTCTGTCTA TTGTACTCCG 
AACAAACCGA GCCGCACGTC CATGAGCAAG ATGTTTGTTA AGGGTGCTCC TGAAGGTGTG 
ATTGACAGAT GTACACATGT CCGTGTTGGA AATGCTAAAA TACCTTTAAC CCCAGGAATT 
AAACAGAAAA TAATGTCTGT CATTAGAGAA TGGGGAACTG GCAGAGACAC ATTGCGCTGC 
TTGGCTCTGG CAACCCATGA CAACCCTCCC AAAAAAGAGG AAATGAATCT GGAGGACTCC 
TCAAATTTCA TTAACTATGA GACCAACCTG ACATTTGTGG GCTGCGTGGG AATGCTGGAT 
CCCCCGAGGA TTGAAGTAGC TTCATCCATA AAGCTGTGCA GACAGGCTGG CATCAGAGTT 
ATTATGATCA CTGGTGACAA CAAAGGCACG GCTGTGGCCA TTTGCCGCCG CATCGGGATC 
TTTGTGGAGG ATGAGGACGT GTCCACCAAA GCCTTCACGG GCCGGGAGTT CGATGAGCTG 
TCCCTGGCTG CACAGAGAGA TGCCTGTCAC CACGCCCGCT GCTTTGCCCG GGTGGAGCCT 
TCCCACAAAT CCAAAATCGT GGAGTTTCTT CAGTCTTTCG ATGAGATCAC AGCCATGACT 
GGTGATGGTG TCAATGATGC CCCTGCACTG AAGAAAGCAG AGATTGGAAT TGCCATGGGT 
TCTGGTACTG CAGTGGCCAA AACTGCTTCT GAAATGGTTT TAGCAGATGA CAACTTCTCC 
ACGATTGTGG CTGCTGTGGA AGAAGGACGT GCAATTTATA ACAACATGAA ACAGTTCATC 
CGATACCTGA TCTCCTCCAA TGTTGGGGAA GTTGTTTGTA TCTTCTTGAC TGCTGCCCTG 
GGTTTTCCTG AAGCTCTGAT TCCTGTTCAG CTGCTGTGGG TAAACCTTGT GACGGATGGG 
CTCCCTGCCA CTGCCCTGGG CTTTAACCCT CCTGACCTCG ATATCATGAA CAAGCCTCCG 
CGCAACCCCA AAGAGCCCCT GATCAGTGGC TGGCTCTTCT TCCGGTACCT GGCTATTGGA 
TGTTATGTTG GTGCTGCCAC AGTGGGTGCT GCTGCTTGGT GGTTTATTGC TGCTGATGGT 
GGTCCAAAAG TTTCCTTTTA CCAGCTGAGC CATTTTCTGC AGTGCAAGGA GGACAACCCA 
GATTTCTATG GGGTGGACTG TGTGGTGTTC GAGTCCCCGT ACCCGATGAC AATGGCTCTG 
TCTGTGCTTG TCACCATTGA GATGTGCAAT GCTCTCAACA GCCTGTCAGA AAACCAGTCC 
CTGATGAGGA TGCCCCCGTG GGAGAACATC TGGCTGGTGG GGGCCATTTG CTTGTCCATG 
TCCCTCCACT TCCTTATCCT TTATGTGGAA CCACTGCCAA TTATCTTCCA GATCACACCT 
CTGAACGTGA CGCAGTGGCT GATGGTGTTG AAAATCTCCC TCCCTGTCAT TCTGCTGGAT 
GAAACACTTA AATATGTGGC CCGTAACTAC CTGGAACCTG CAATACTGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP2A2 ( XM_005055123.2 ) cDNA ORF clone, Ficedula albicollis(collared flycatcher) -> XP_005055180.1 Ficedula albicollis ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 (ATP2A2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OFi05421
Clone ID Related Accession (Same CDS sequence)	XM_005055123.2
Accession Version	XM_005055123.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-19
Organism	Ficedula albicollis(collared flycatcher)
Product	sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_021687.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 20, 2016 this sequence version replaced XM_005055123.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ficedula albicollis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACG CGCACACAAA GACTGTGGAG GAAGTTTTGG CTTATTTCGG CGTCAACGAG 
AGCACCGGGC TCAGCCTCGA GCAAGTGAAG AAGCTGAAGG AGAAATGGGG CTCCAACGAG 
CTGCCGGCAG AGGAAGGAAA AACCTTACTT GAGCTTGTGA TTGAACAATT TGAAGACTTA 
CTAGTTAGAA TTTTGTTGCT GGCTGCTTGC ATATCTTTTG TCCTGGCCTG GTTTGAAGAA 
GGTGAAGAAA CAATCACAGC ATTTGTAGAG CCTTTTGTAA TCTTACTTAT ATTAGTAGCC 
AATGCTATTG TGGGAGTGTG GCAGGAAAGA AATGCTGAAA ATGCTATCGA AGCTCTTAAA 
GAATATGAGC CAGAGATGGG CAAAGTGTAT CGACAGGACA GAAAAAGTGT CCAGAGGATT 
AAAGCAAGAG ACATTGTCCC GGGTGATATC GTGGAAGTGG CAGTTGGAGA CAAGGTTCCT 
GCTGATATAA GAATTACTTC TATCAAATCT ACAACTCTAA GGGTAGACCA GTCAATCCTT 
ACAGGTGAAT CTGTGTCTGT TATTAAGCAC ACAGACCCTG TGCCTGATCC TCGTGCTGTA 
AATCAGGACA AGAAGAACAT GCTCTTTTCT GGTACAAACA TTGCTGCTGG TAAGGCCATG 
GGGGTGGTTA TTGCAACAGG GGTCAACACT GAAATCGGGA AGATCCGTGA CGAGATGGTG 
GCGACGGAGC AGGAACGAAC GCCCCTGCAG CAGAAGCTGG ACGAGTTTGG GGAGCAGCTG 
TCCAAGGTCA TCTCGCTGAT CTGCATCGCC GTGTGGATCA TCAACATCGG CCACTTCAAC 
GACCCCGTGC ACGGCGGCTC CTGGATCCGC GGCGCCATCT ACTACTTCAA AATCGCCGTG 
GCGCTGGCGG TGGCCGCCAT CCCCGAGGGG CTGCCCGCCG TCATCACCAC CTGCCTGGCG 
CTGGGCACGC GCCGCATGGC CAAGAAGAAC GCCATCGTCA GGAGCCTGCC CTCCGTGGAG 
ACCCTGGGCT GCACCTCCGT CATCTGCTCC GACAAGACCG GCACCCTCAC CACCAACCAG 
ATGTCCGTGT GCAGGATGTT TATCCTGGAC AGAGTAGAAG GAGACAGCTG CTCTCTGAAT 
GAATTTACTG TAACTGGTTC AACATATGCT CCCATGGGTG AAGTGCATAA AGATGACAAA 
CTCATTAAAT GCAGCCAGTA TGATGGTCTT GTGGAACTTG CAACGATCTG TGCACTCTGC 
AATGATTCCT CTTTGGATTA CAATGAAGCT AAGGGAGTTT ATGAAAAGGT TGGTGAAGCC 
ACAGAGACGG CGCTCACGTG TCTGGTTGAG AAGATGAACG TGTTTGACAC AGACCTGAAG 
GGACTTTCTA GAATCGAACG TGCCAACGCC TGCAACTCGG TGATAAAACA ACTCATGAAG 
AAAGAATTCA CTCTGGAATT CTCAAGAGAC AGGAAGTCGA TGTCTGTCTA TTGTACTCCG 
AACAAACCGA GCCGCACGTC CATGAGCAAG ATGTTTGTTA AGGGTGCTCC TGAAGGTGTG 
ATTGACAGAT GTACACATGT CCGTGTTGGA AATGCTAAAA TACCTTTAAC CCCAGGAATT 
AAACAGAAAA TAATGTCTGT CATTAGAGAA TGGGGAACTG GCAGAGACAC ATTGCGCTGC 
TTGGCTCTGG CAACCCATGA CAACCCTCCC AAAAAAGAGG AAATGAATCT GGAGGACTCC 
TCAAATTTCA TTAACTATGA GACCAACCTG ACATTTGTGG GCTGCGTGGG AATGCTGGAT 
CCCCCGAGGA TTGAAGTAGC TTCATCCATA AAGCTGTGCA GACAGGCTGG CATCAGAGTT 
ATTATGATCA CTGGTGACAA CAAAGGCACG GCTGTGGCCA TTTGCCGCCG CATCGGGATC 
TTTGTGGAGG ATGAGGACGT GTCCACCAAA GCCTTCACGG GCCGGGAGTT CGATGAGCTG 
TCCCTGGCTG CACAGAGAGA TGCCTGTCAC CACGCCCGCT GCTTTGCCCG GGTGGAGCCT 
TCCCACAAAT CCAAAATCGT GGAGTTTCTT CAGTCTTTCG ATGAGATCAC AGCCATGACT 
GGTGATGGTG TCAATGATGC CCCTGCACTG AAGAAAGCAG AGATTGGAAT TGCCATGGGT 
TCTGGTACTG CAGTGGCCAA AACTGCTTCT GAAATGGTTT TAGCAGATGA CAACTTCTCC 
ACGATTGTGG CTGCTGTGGA AGAAGGACGT GCAATTTATA ACAACATGAA ACAGTTCATC 
CGATACCTGA TCTCCTCCAA TGTTGGGGAA GTTGTTTGTA TCTTCTTGAC TGCTGCCCTG 
GGTTTTCCTG AAGCTCTGAT TCCTGTTCAG CTGCTGTGGG TAAACCTTGT GACGGATGGG 
CTCCCTGCCA CTGCCCTGGG CTTTAACCCT CCTGACCTCG ATATCATGAA CAAGCCTCCG 
CGCAACCCCA AAGAGCCCCT GATCAGTGGC TGGCTCTTCT TCCGGTACCT GGCTATTGGA 
TGTTATGTTG GTGCTGCCAC AGTGGGTGCT GCTGCTTGGT GGTTTATTGC TGCTGATGGT 
GGTCCAAAAG TTTCCTTTTA CCAGCTGAGC CATTTTCTGC AGTGCAAGGA GGACAACCCA 
GATTTCTATG GGGTGGACTG TGTGGTGTTC GAGTCCCCGT ACCCGATGAC AATGGCTCTG 
TCTGTGCTTG TCACCATTGA GATGTGCAAT GCTCTCAACA GCCTGTCAGA AAACCAGTCC 
CTGATGAGGA TGCCCCCGTG GGAGAACATC TGGCTGGTGG GGGCCATTTG CTTGTCCATG 
TCCCTCCACT TCCTTATCCT TTATGTGGAA CCACTGCCAA TTATCTTCCA GATCACACCT 
CTGAACGTGA CGCAGTGGCT GATGGTGTTG AAAATCTCCC TCCCTGTCAT TCTGCTGGAT 
GAAACACTTA AATATGTGGC CCGTAACTAC CTGGAACCTG GTAAAGATGA TAGTGTGCGG 
CCTGCCACCA AACCCTGTGC TCTGTCAGCA TGCACCGAAG GAGTTTCCTG GCCGTTTGTG 
TTCATTACTA TGCCCCTGGT TATCTGGCTT TACAGCACAG ACACTAACTT TAGTGATATG 
TTCTGGTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005055180.1
CDS	218..3349
Translation	MENAHTKTVEEVLAYFGVNESTGLSLEQVKKLKEKWGSNELPAEEGKTLLELVIEQFEDLLVRILLLAACISFVLAWFEEGEETITAFVEPFVILLILVANAIVGVWQERNAENAIEALKEYEPEMGKVYRQDRKSVQRIKARDIVPGDIVEVAVGDKVPADIRITSIKSTTLRVDQSILTGESVSVIKHTDPVPDPRAVNQDKKNMLFSGTNIAAGKAMGVVIATGVNTEIGKIRDEMVATEQERTPLQQKLDEFGEQLSKVISLICIAVWIINIGHFNDPVHGGSWIRGAIYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFILDRVEGDSCSLNEFTVTGSTYAPMGEVHKDDKLIKCSQYDGLVELATICALCNDSSLDYNEAKGVYEKVGEATETALTCLVEKMNVFDTDLKGLSRIERANACNSVIKQLMKKEFTLEFSRDRKSMSVYCTPNKPSRTSMSKMFVKGAPEGVIDRCTHVRVGNAKIPLTPGIKQKIMSVIREWGTGRDTLRCLALATHDNPPKKEEMNLEDSSNFINYETNLTFVGCVGMLDPPRIEVASSIKLCRQAGIRVIMITGDNKGTAVAICRRIGIFVEDEDVSTKAFTGREFDELSLAAQRDACHHARCFARVEPSHKSKIVEFLQSFDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGFPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMNKPPRNPKEPLISGWLFFRYLAIGCYVGAATVGAAAWWFIAADGGPKVSFYQLSHFLQCKEDNPDFYGVDCVVFESPYPMTMALSVLVTIEMCNALNSLSENQSLMRMPPWENIWLVGAICLSMSLHFLILYVEPLPIIFQITPLNVTQWLMVLKISLPVILLDETLKYVARNYLEPGKDDSVRPATKPCALSACTEGVSWPFVFITMPLVIWLYSTDTNFSDMFWS

Target ORF information:

RefSeq Version	XM_005055123.2
Organism	Ficedula albicollis(collared flycatcher)
Definition	Ficedula albicollis ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 (ATP2A2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005055123.2

ORF Insert Sequence:

ATGGAGAACG CGCACACAAA GACTGTGGAG GAAGTTTTGG CTTATTTCGG CGTCAACGAG 
AGCACCGGGC TCAGCCTCGA GCAAGTGAAG AAGCTGAAGG AGAAATGGGG CTCCAACGAG 
CTGCCGGCAG AGGAAGGAAA AACCTTACTT GAGCTTGTGA TTGAACAATT TGAAGACTTA 
CTAGTTAGAA TTTTGTTGCT GGCTGCTTGC ATATCTTTTG TCCTGGCCTG GTTTGAAGAA 
GGTGAAGAAA CAATCACAGC ATTTGTAGAG CCTTTTGTAA TCTTACTTAT ATTAGTAGCC 
AATGCTATTG TGGGAGTGTG GCAGGAAAGA AATGCTGAAA ATGCTATCGA AGCTCTTAAA 
GAATATGAGC CAGAGATGGG CAAAGTGTAT CGACAGGACA GAAAAAGTGT CCAGAGGATT 
AAAGCAAGAG ACATTGTCCC GGGTGATATC GTGGAAGTGG CAGTTGGAGA CAAGGTTCCT 
GCTGATATAA GAATTACTTC TATCAAATCT ACAACTCTAA GGGTAGACCA GTCAATCCTT 
ACAGGTGAAT CTGTGTCTGT TATTAAGCAC ACAGACCCTG TGCCTGATCC TCGTGCTGTA 
AATCAGGACA AGAAGAACAT GCTCTTTTCT GGTACAAACA TTGCTGCTGG TAAGGCCATG 
GGGGTGGTTA TTGCAACAGG GGTCAACACT GAAATCGGGA AGATCCGTGA CGAGATGGTG 
GCGACGGAGC AGGAACGAAC GCCCCTGCAG CAGAAGCTGG ACGAGTTTGG GGAGCAGCTG 
TCCAAGGTCA TCTCGCTGAT CTGCATCGCC GTGTGGATCA TCAACATCGG CCACTTCAAC 
GACCCCGTGC ACGGCGGCTC CTGGATCCGC GGCGCCATCT ACTACTTCAA AATCGCCGTG 
GCGCTGGCGG TGGCCGCCAT CCCCGAGGGG CTGCCCGCCG TCATCACCAC CTGCCTGGCG 
CTGGGCACGC GCCGCATGGC CAAGAAGAAC GCCATCGTCA GGAGCCTGCC CTCCGTGGAG 
ACCCTGGGCT GCACCTCCGT CATCTGCTCC GACAAGACCG GCACCCTCAC CACCAACCAG 
ATGTCCGTGT GCAGGATGTT TATCCTGGAC AGAGTAGAAG GAGACAGCTG CTCTCTGAAT 
GAATTTACTG TAACTGGTTC AACATATGCT CCCATGGGTG AAGTGCATAA AGATGACAAA 
CTCATTAAAT GCAGCCAGTA TGATGGTCTT GTGGAACTTG CAACGATCTG TGCACTCTGC 
AATGATTCCT CTTTGGATTA CAATGAAGCT AAGGGAGTTT ATGAAAAGGT TGGTGAAGCC 
ACAGAGACGG CGCTCACGTG TCTGGTTGAG AAGATGAACG TGTTTGACAC AGACCTGAAG 
GGACTTTCTA GAATCGAACG TGCCAACGCC TGCAACTCGG TGATAAAACA ACTCATGAAG 
AAAGAATTCA CTCTGGAATT CTCAAGAGAC AGGAAGTCGA TGTCTGTCTA TTGTACTCCG 
AACAAACCGA GCCGCACGTC CATGAGCAAG ATGTTTGTTA AGGGTGCTCC TGAAGGTGTG 
ATTGACAGAT GTACACATGT CCGTGTTGGA AATGCTAAAA TACCTTTAAC CCCAGGAATT 
AAACAGAAAA TAATGTCTGT CATTAGAGAA TGGGGAACTG GCAGAGACAC ATTGCGCTGC 
TTGGCTCTGG CAACCCATGA CAACCCTCCC AAAAAAGAGG AAATGAATCT GGAGGACTCC 
TCAAATTTCA TTAACTATGA GACCAACCTG ACATTTGTGG GCTGCGTGGG AATGCTGGAT 
CCCCCGAGGA TTGAAGTAGC TTCATCCATA AAGCTGTGCA GACAGGCTGG CATCAGAGTT 
ATTATGATCA CTGGTGACAA CAAAGGCACG GCTGTGGCCA TTTGCCGCCG CATCGGGATC 
TTTGTGGAGG ATGAGGACGT GTCCACCAAA GCCTTCACGG GCCGGGAGTT CGATGAGCTG 
TCCCTGGCTG CACAGAGAGA TGCCTGTCAC CACGCCCGCT GCTTTGCCCG GGTGGAGCCT 
TCCCACAAAT CCAAAATCGT GGAGTTTCTT CAGTCTTTCG ATGAGATCAC AGCCATGACT 
GGTGATGGTG TCAATGATGC CCCTGCACTG AAGAAAGCAG AGATTGGAAT TGCCATGGGT 
TCTGGTACTG CAGTGGCCAA AACTGCTTCT GAAATGGTTT TAGCAGATGA CAACTTCTCC 
ACGATTGTGG CTGCTGTGGA AGAAGGACGT GCAATTTATA ACAACATGAA ACAGTTCATC 
CGATACCTGA TCTCCTCCAA TGTTGGGGAA GTTGTTTGTA TCTTCTTGAC TGCTGCCCTG 
GGTTTTCCTG AAGCTCTGAT TCCTGTTCAG CTGCTGTGGG TAAACCTTGT GACGGATGGG 
CTCCCTGCCA CTGCCCTGGG CTTTAACCCT CCTGACCTCG ATATCATGAA CAAGCCTCCG 
CGCAACCCCA AAGAGCCCCT GATCAGTGGC TGGCTCTTCT TCCGGTACCT GGCTATTGGA 
TGTTATGTTG GTGCTGCCAC AGTGGGTGCT GCTGCTTGGT GGTTTATTGC TGCTGATGGT 
GGTCCAAAAG TTTCCTTTTA CCAGCTGAGC CATTTTCTGC AGTGCAAGGA GGACAACCCA 
GATTTCTATG GGGTGGACTG TGTGGTGTTC GAGTCCCCGT ACCCGATGAC AATGGCTCTG 
TCTGTGCTTG TCACCATTGA GATGTGCAAT GCTCTCAACA GCCTGTCAGA AAACCAGTCC 
CTGATGAGGA TGCCCCCGTG GGAGAACATC TGGCTGGTGG GGGCCATTTG CTTGTCCATG 
TCCCTCCACT TCCTTATCCT TTATGTGGAA CCACTGCCAA TTATCTTCCA GATCACACCT 
CTGAACGTGA CGCAGTGGCT GATGGTGTTG AAAATCTCCC TCCCTGTCAT TCTGCTGGAT 
GAAACACTTA AATATGTGGC CCGTAACTAC CTGGAACCTG GTAAAGATGA TAGTGTGCGG 
CCTGCCACCA AACCCTGTGC TCTGTCAGCA TGCACCGAAG GAGTTTCCTG GCCGTTTGTG 
TTCATTACTA TGCCCCTGGT TATCTGGCTT TACAGCACAG ACACTAACTT TAGTGATATG 
TTCTGGTCTT GA