ZW10 cDNA ORF clone, Eurypyga helias(sunbittern)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZW10
Entrez Gene ID	104502663
Full Name	zw10 kinetochore protein
Gene Type	protein-coding
Organism	Eurypyga helias(sunbittern)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010149995.1	XP_010148297.1	centromere/kinetochore protein zw10 homolog

Pathways from BioSystems
Gene Ontology

The following ZW10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZW10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEu97936	XM_010149995.1 Latest version!	Eurypyga helias zw10 kinetochore protein (ZW10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZW10 ( XM_010149995.1 ) cDNA ORF clone, Eurypyga helias(sunbittern) -> XP_010148297.1 Eurypyga helias zw10 kinetochore protein (ZW10), mRNA.

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CloneID	OEu97936
Clone ID Related Accession (Same CDS sequence)	XM_010149995.1
Accession Version	XM_010149995.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-05
Organism	Eurypyga helias(sunbittern)
Product	centromere/kinetochore protein zw10 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010245248.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Eurypyga helias Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTAACA AGAAGTACAA CGAGTTCCTG CCCAGCATGC AGAGCGCCGA GGACTTGGTG 
TCGCAGGTGG ATGGGCTGTC CAGCAACATC GACCTGCTGA AAGCGGGGAT TGAAAACGAG 
GTTCAGCGAG ATCTAAACGT CGCTGTTGCC GAATTCACCG AGTTGAAGCA GCAGTTGGAG 
CGAGACACAC TGGTTCTGAG TATCCTGAAA AAGCTACAGG AGTTTGATAC AGCTATTAAA 
GAGTACAATA CTGCATTGCT GGAGAAGAAG TACGTTGCAG CAGCTCAACA GCTGGAAAAG 
GCCCGGAGCA TCCTGAAAAT GCTGGAATCC CGCAAGGGCT TCGAGCTGAA GATCCTGAAA 
GCACTAGGCA CAGAGCTGAC AGTGCAGACA CAGAACATGC TCTATCATCT AGGAGAAGAA 
TGGCAGAACC TGGCCGTGTG GAAGCTTCCT CCTTCAAAAG AAAGCAGCAG CCTGGAGTCG 
GTGCTGCATG CAGAGCTGCA CTTACGCACA CTGCCATCAA AAGAGGAAGA GATTGCTGGC 
CCACCTGTCG CTTCTGTGCT GCAGGCCTTT GCTGTCCTTG GAGAACTGCA CACCAAGCTT 
AAAAGTTTTG GCCAGCTGTT GCTGAAGTAC ATCCTCAAGC CACTTATTTC GTACCCATCT 
CTCCGGCCGG TCACAGAGGA ACAGTCAGAT GGGTTCATCC TGCGTTTTAA GTCCGAGGAG 
CCCAACTTGG ATCATTCCTC TCCTGTGGAG GTTTTTGCCA AAATCAAGCT TGTTTTTGAA 
GTTCTCCACA AATATCTCCT GAATGTGCCT CTCGAGCAGC CTGTGGAAGA CAAAGAGGAG 
CGCGGAGTGA CGCTTGCAGA ACTGCTCGGT GATACCATAT GGGCAGAGTT ATCGGATTGC 
CTCATCCAGG ACTGCCTGGT GAATTCCATC CCAACCAACA GCAGCAAACT GGAGCAGTAT 
ATAGAGGTGA TTAAATCCAC AGAGGAATTT GAGGAAGCCT TGAAGAACAT GCAGTTTTTG 
AAAGGAGACA CAACTGATTT ACTGAAATAC GCCCGTAATG TCAATTCCCA CTTTGCTAAC 
AAGAAGTGCC AGGATGTGAT TGTGGCAGCC AGAACCCTGA TGACCTCAGA AATACACAAT 
ACTGTAAAGA TCACGCCCGA TTCCTGCGTA GCCCTGCCAA AGCTTCCTGA TTCTGGGGCA 
GGAGATCCGT TAACAATGCA AAACGCTTCC AAGCCTCAGC ATACCAACAC GGTGAATTTG 
GAGAACGAGA CTAAACTGAG CCAGTACACG TTTTCTCTGC CCACATGCCG CATCAGTTCG 
TCCGTGGAGA AGCTAATGGA GCTGGCTTAC CAGACGCTCT CAGAGGCTAC GGCCAGCACG 
GATCAGTGCT GCATACAGCT CTTCTACGCT GTGCGGAACA TATTCCAGCT CTTCTATGAC 
GTAGTGCCAA CATACCACAA AGAGAATCTT CAGAAGTTAC CCCAGCTCGC AGCCATTCAC 
CACAACAACT GCATGTATAT TGCTCACCAC TTGCTGACCC TGGGACACCA GTTCCGATAC 
CGCATGACCA ACATCCTGTG CAACGGAGCT GCTACTTTTG TCGATCTGGT ACCTGGTTTT 
AGGAGACTTG GGATGGAGTG TTTCCTGGCC CAGATGCGAG TACAGAAAGG AGAAATCCTG 
GAGAGGCTGT CAAGTGCCAG GAATTTTTCT AATATGGATG ATGAGGAAAA TTACTGTGCA 
GCAAACAAGG CAATAAGGCA GGTACTGCAC CAGCTGAAAA GACTGGGGAA GGTCTGGCAG 
GACGTCCTAC CAGTGAACGT GTACTGCAAG GCTATGGGGA CTTTACTGAA CACGGCGCTC 
TCGGAGGTGG TCACGAGGAT TGCTGCCCTA GAGGACATCT CTGCGGAAGA TGCAGACAGG 
TTGTACTCCC TCTGTAGAAT CTTGGTGGAG GAAGGACCCC AAGTTTTCAC CCCACTCCTC 
GAGGAAGACA AAAATAAGAC ATACCAGCAG GAAGTTCCAG TCTACGTGCA GAAGTGGATG 
ACGTTCAAAG AGCTGATGAT CATCCTACAA GCCAACCTCC AAGAAATAGT AGATCAGTGG 
GCGGATGGGA AGGGGCCTCT CGCAGCTGAA TTTTCCGCAG CCGAAGCGAA GAGCCTGATC 
CGAGCCTTGT TCCAGAACAC GGAAAGGAGA GCAGCAGCAC TGGCCAAAAT CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010148297.1
CDS	10..2226
Translation	MINKKYNEFLPSMQSAEDLVSQVDGLSSNIDLLKAGIENEVQRDLNVAVAEFTELKQQLERDTLVLSILKKLQEFDTAIKEYNTALLEKKYVAAAQQLEKARSILKMLESRKGFELKILKALGTELTVQTQNMLYHLGEEWQNLAVWKLPPSKESSSLESVLHAELHLRTLPSKEEEIAGPPVASVLQAFAVLGELHTKLKSFGQLLLKYILKPLISYPSLRPVTEEQSDGFILRFKSEEPNLDHSSPVEVFAKIKLVFEVLHKYLLNVPLEQPVEDKEERGVTLAELLGDTIWAELSDCLIQDCLVNSIPTNSSKLEQYIEVIKSTEEFEEALKNMQFLKGDTTDLLKYARNVNSHFANKKCQDVIVAARTLMTSEIHNTVKITPDSCVALPKLPDSGAGDPLTMQNASKPQHTNTVNLENETKLSQYTFSLPTCRISSSVEKLMELAYQTLSEATASTDQCCIQLFYAVRNIFQLFYDVVPTYHKENLQKLPQLAAIHHNNCMYIAHHLLTLGHQFRYRMTNILCNGAATFVDLVPGFRRLGMECFLAQMRVQKGEILERLSSARNFSNMDDEENYCAANKAIRQVLHQLKRLGKVWQDVLPVNVYCKAMGTLLNTALSEVVTRIAALEDISAEDADRLYSLCRILVEEGPQVFTPLLEEDKNKTYQQEVPVYVQKWMTFKELMIILQANLQEIVDQWADGKGPLAAEFSAAEAKSLIRALFQNTERRAAALAKIK

Target ORF information:

RefSeq Version	XM_010149995.1
Organism	Eurypyga helias(sunbittern)
Definition	Eurypyga helias zw10 kinetochore protein (ZW10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010149995.1

ORF Insert Sequence:

ATGATTAACA AGAAGTACAA CGAGTTCCTG CCCAGCATGC AGAGCGCCGA GGACTTGGTG 
TCGCAGGTGG ATGGGCTGTC CAGCAACATC GACCTGCTGA AAGCGGGGAT TGAAAACGAG 
GTTCAGCGAG ATCTAAACGT CGCTGTTGCC GAATTCACCG AGTTGAAGCA GCAGTTGGAG 
CGAGACACAC TGGTTCTGAG TATCCTGAAA AAGCTACAGG AGTTTGATAC AGCTATTAAA 
GAGTACAATA CTGCATTGCT GGAGAAGAAG TACGTTGCAG CAGCTCAACA GCTGGAAAAG 
GCCCGGAGCA TCCTGAAAAT GCTGGAATCC CGCAAGGGCT TCGAGCTGAA GATCCTGAAA 
GCACTAGGCA CAGAGCTGAC AGTGCAGACA CAGAACATGC TCTATCATCT AGGAGAAGAA 
TGGCAGAACC TGGCCGTGTG GAAGCTTCCT CCTTCAAAAG AAAGCAGCAG CCTGGAGTCG 
GTGCTGCATG CAGAGCTGCA CTTACGCACA CTGCCATCAA AAGAGGAAGA GATTGCTGGC 
CCACCTGTCG CTTCTGTGCT GCAGGCCTTT GCTGTCCTTG GAGAACTGCA CACCAAGCTT 
AAAAGTTTTG GCCAGCTGTT GCTGAAGTAC ATCCTCAAGC CACTTATTTC GTACCCATCT 
CTCCGGCCGG TCACAGAGGA ACAGTCAGAT GGGTTCATCC TGCGTTTTAA GTCCGAGGAG 
CCCAACTTGG ATCATTCCTC TCCTGTGGAG GTTTTTGCCA AAATCAAGCT TGTTTTTGAA 
GTTCTCCACA AATATCTCCT GAATGTGCCT CTCGAGCAGC CTGTGGAAGA CAAAGAGGAG 
CGCGGAGTGA CGCTTGCAGA ACTGCTCGGT GATACCATAT GGGCAGAGTT ATCGGATTGC 
CTCATCCAGG ACTGCCTGGT GAATTCCATC CCAACCAACA GCAGCAAACT GGAGCAGTAT 
ATAGAGGTGA TTAAATCCAC AGAGGAATTT GAGGAAGCCT TGAAGAACAT GCAGTTTTTG 
AAAGGAGACA CAACTGATTT ACTGAAATAC GCCCGTAATG TCAATTCCCA CTTTGCTAAC 
AAGAAGTGCC AGGATGTGAT TGTGGCAGCC AGAACCCTGA TGACCTCAGA AATACACAAT 
ACTGTAAAGA TCACGCCCGA TTCCTGCGTA GCCCTGCCAA AGCTTCCTGA TTCTGGGGCA 
GGAGATCCGT TAACAATGCA AAACGCTTCC AAGCCTCAGC ATACCAACAC GGTGAATTTG 
GAGAACGAGA CTAAACTGAG CCAGTACACG TTTTCTCTGC CCACATGCCG CATCAGTTCG 
TCCGTGGAGA AGCTAATGGA GCTGGCTTAC CAGACGCTCT CAGAGGCTAC GGCCAGCACG 
GATCAGTGCT GCATACAGCT CTTCTACGCT GTGCGGAACA TATTCCAGCT CTTCTATGAC 
GTAGTGCCAA CATACCACAA AGAGAATCTT CAGAAGTTAC CCCAGCTCGC AGCCATTCAC 
CACAACAACT GCATGTATAT TGCTCACCAC TTGCTGACCC TGGGACACCA GTTCCGATAC 
CGCATGACCA ACATCCTGTG CAACGGAGCT GCTACTTTTG TCGATCTGGT ACCTGGTTTT 
AGGAGACTTG GGATGGAGTG TTTCCTGGCC CAGATGCGAG TACAGAAAGG AGAAATCCTG 
GAGAGGCTGT CAAGTGCCAG GAATTTTTCT AATATGGATG ATGAGGAAAA TTACTGTGCA 
GCAAACAAGG CAATAAGGCA GGTACTGCAC CAGCTGAAAA GACTGGGGAA GGTCTGGCAG 
GACGTCCTAC CAGTGAACGT GTACTGCAAG GCTATGGGGA CTTTACTGAA CACGGCGCTC 
TCGGAGGTGG TCACGAGGAT TGCTGCCCTA GAGGACATCT CTGCGGAAGA TGCAGACAGG 
TTGTACTCCC TCTGTAGAAT CTTGGTGGAG GAAGGACCCC AAGTTTTCAC CCCACTCCTC 
GAGGAAGACA AAAATAAGAC ATACCAGCAG GAAGTTCCAG TCTACGTGCA GAAGTGGATG 
ACGTTCAAAG AGCTGATGAT CATCCTACAA GCCAACCTCC AAGAAATAGT AGATCAGTGG 
GCGGATGGGA AGGGGCCTCT CGCAGCTGAA TTTTCCGCAG CCGAAGCGAA GAGCCTGATC 
CGAGCCTTGT TCCAGAACAC GGAAAGGAGA GCAGCAGCAC TGGCCAAAAT CAAGTAA