MIB2 cDNA ORF clone, Erinaceus europaeus(western European hedgehog)

Gene
Clones
gRNAs

Summary

Gene Symbol	MIB2
Entrez Gene ID	103119230
Full Name	mindbomb E3 ubiquitin protein ligase 2
Gene Type	protein-coding
Organism	Erinaceus europaeus(western European hedgehog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007529486.2	XP_007529548.2	E3 ubiquitin-protein ligase MIB2 isoform X2
XM_007529490.2	XP_007529552.2	E3 ubiquitin-protein ligase MIB2 isoform X3
XM_016191017.1	XP_016046503.1	E3 ubiquitin-protein ligase MIB2 isoform X5
XM_016191015.1	XP_016046501.1	E3 ubiquitin-protein ligase MIB2 isoform X1
XM_016191016.1	XP_016046502.1	E3 ubiquitin-protein ligase MIB2 isoform X4

Pathways from BioSystems
Gene Ontology

The following MIB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MIB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OEr17581	XM_016191015.1 Latest version!	Erinaceus europaeus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OEr17582	XM_007529486.2 Latest version!	Erinaceus europaeus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OEr17583	XM_007529490.2 Latest version!	Erinaceus europaeus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OEr17584	XM_016191016.1 Latest version!	Erinaceus europaeus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OEr17585	XM_016191017.1 Latest version!	Erinaceus europaeus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MIB2 ( XM_016191015.1 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_016046501.1 Erinaceus europaeus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OEr17581
Clone ID Related Accession (Same CDS sequence)	XM_016191015.1
Accession Version	XM_016191015.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	E3 ubiquitin-protein ligase MIB2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006804353.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCATT GCTCTTTGGT CATGACTGTG TTGCCCTGTG TTCCTTGCAG GCTCAGCTTC 
TGCCACTTTA TTCTTCAGGA CAAAGGTGCA GAGCCACTGC CCATCATGGA TCCAGACCCA 
CAGGCGGGTG TGCAGGTGGG CATGCGCGTT GTCCGTGGCG TGGACTGGAA GTGGGCCCAG 
CAGGACGGTG GCGAGGGCGG CGTGGGTACG GTGGTGGAAC TCGGCCGCCA CGGCAGCCCC 
TCCACCCCTG ACCGAACAGT GGTTGTGCAG TGGGACCATG GCACCCGCAC CAACTACCGT 
GCGGGCTATC AGGGTGCCCA TGACCTGCTG CTCTACGACA ATGCCCAGAT CGGTGTGCGG 
CACCCCAACA TCATCTGCGA CTGCTGCAAG AAACACGGGT TGCGGGGCAT GCGCTGGAAG 
TGCCTCGTCT GTTTTGACTA TGACCTGTGC ACTCAGTGTT ACATGCACAA CAGACATGAT 
CTGGCACACG CCTTTGAGCG CTACGAGACT GCACACTCGC GCCCGGTGAC GCTGAGCCCC 
CGCCAGGGCC TCCCGAGGGT TCCACTACGG GGCATCTTCC AGGGTGCGAA GGTGGTGCGG 
GGTCCTGACT GGGAGTGGGG CTCCCAGGAT GGAGGGGAGG GGAAACCAGG CCGTGTGGTG 
GACATCCGGG GCTGGGACGT GGAGACAGGC CGGAGTGTGG CCAGCGTGAC CTGGGCAGAT 
GGCACCACCA ACGTGTACCG CGTAGGCCAC AAGGGCAAGG TGGACCTCAA GTGTGTGGGC 
GAGGCAGCCG GCGGCTTCTA CTACAAGGAG CACCTCCCCA GGCTGGCCTG CTATCCCCCA 
GGCAAGCCGG CTGAACTGCG ACGCAGGGTG AGTGTGGATG GGCAGCCCTT CCAGCACGGC 
GACAAGGTCA AGTGCCTGCT GGACTCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGT 
GGCTGGAATC CCCGGATGGC GGAGTGCATT GGACAGACGG GCACCGTGCA CCGCGTCACA 
GACCGTGGGG ATGTGCGTGT GCAGTTCAGC CACGAGACCC GTTGGACCTT CCACCCAGGG 
GCTCTCACCA AGCATGACTG CTTCTGGGTG GGTGATGTGG TGCGGGTCAT CGACGACCTG 
GACATGGTGA AGCGGCTGCA GGCTGGGCAT GGCGAGTGGA CAGACGACAT GGCCCCCGCC 
CTGGGCCGTG TAGGAAAGGT GGTGAAGGTC TTTGGCGACG GGAACCTGCG TGTGGCAGTC 
AGCGGCCAGC TGTGGACCTT CAGCCCCTCA TGCCTCGTGG CCTACCGGCC CGAAGAGAAC 
ACCAACCTGG ACGTCGCTGA GCGAGCCCGG GAGAACAAGA GCTCCCTGAG TGTTGCCCTG 
GACAAACTTC GAGCCCAGAA GAGTGGTGAC CTGGAGCAAC CGGGCAGGCT GGTGGTGGAG 
GTGGCACTGG GCAACTTGTC ACGGGCTGTG GACCTGCTGC GGAGGCACCC AGAGCAGGTG 
GATACCAAGA ATCAGGGCCG GACTGCCCTG CAGGTGGCTG CCTACCTGGG CCAGGTGAAG 
CTGGTGCGGC TGCTGCTCCA GGCAAGGGCA GGCACAGACC TGCCTGATGA GGAGGGCAAC 
ACTTCACTGC ACTATGCAGC CCTAGGAAAC CAGCCGGAGG TCACCCAGGT GCTCCTGAGT 
GTGGGGTGCC CGGCTGACAC CCTCAACAGC AGCCAGAGCA CTGCACTACA CCTTGCAGTG 
CAGAGGGGCT TCCTGGAGGT GGTGCGGGTC CTGTGTGAGC ATGGCTGTGA TGTCAACCTG 
CCTGACTCGA ATGCCGACAC ACCACTGCAC TGCGCCATCT CAGCTGGCAC GGGGGCCAGT 
GGCATAGTGG AGGTCCTCAC TGAGGTGCCA GGTGTGGACG TCACCACCAC CAACAGCCAG 
GGCTTCACCC TGCTGCATCA CGCCTCCCTC AAGGGCCACA CGCTCGCTGT CAGGAGGATC 
CTGGCCCGGG CCCGGCAGCT GGTGGATGCC AAGAAGGAGG ACGGCTTCAC TGCCTTGCAC 
CTGGCCGCCC TCAACAATCA CCGAGAGGTG GCCCAGATTC TCATCCGGGA GGGCCACTGC 
GATGTGAATG TGCGTAACCG CAAGCTGCAG ACACCCCTGC ATCTGGCTGT TCAGCAGGCC 
CACATGGGGC TGGTGCCCCT GCTGACCGAC GCTGGCTGCA GCATCAATGC AGAGGATGAG 
GAGGGGGACA CAGCCCTGCA TGTGGCCCTG CAGCGGCACC AGCTGCTGCC CCTGGCAGCT 
GACCGGGCCG GGGGGGACTC TGGGCCAGCA CAGCTATTGT CCAGGCTGCA GGCCTCGGGC 
CTCCCTGGCT TTGCGGAGTT GACAATAGGC ACTGCCATCG CCTGCTTCCT CGCACTGGAG 
GGAGCCGATT TGAGCTACGC CAACCACCGT GGCCGCAGCC CGCTGGACCT GGCTGCAGAG 
GGCCGAGTGC TCAAGGCCCT GCAGGGCTGT GCCCAGCGCT TCCGGGAGCG GCAGGTGGGC 
GCCACGGCCG CCGGCCCCAG GCAGTTGCGG AGCACCCCTA ACACCGTGAC CAACCTGCAT 
GTGGCCCCAG CGGCAGGGCA TGAGGCGGCC GAGTGCCTCG TGTGCTCGGA GCTGGCGCTG 
CTGGTGCTTT TCACGCCGTG CCAGCATCGC ACTGTGTGCG AGGAGTGCGC GCGCAGGATG 
AAGAAGTGCA TCAGGTGCCA GGTGGCCATC AGCAAGAAGC TGCGCCCAGA CGGCTCGGAG 
TTGGCCAGCG CAGCCCCTGC GTCTATGCCG CAGCGGCAGC TTGTAGAGGA GCTGCAGAGC 
CGTTACCGGC AGATGGAGGA GCGCATCACC TGCCCAATCT GCATCGACAA CCACATCCGC 
CTGGTTTTCC AGTGCGGCCA CGGCGCCTGC GCGCCCTGCG GCGCTGCGCT CACTGCCTGC 
CCCATCTGCC GCCAGCCGGT CCGCGACCGA ATCCAGATCT TCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016046501.1
CDS	419..3406
Translation	MHHCSLVMTVLPCVPCRLSFCHFILQDKGAEPLPIMDPDPQAGVQVGMRVVRGVDWKWAQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCLVCFDYDLCTQCYMHNRHDLAHAFERYETAHSRPVTLSPRQGLPRVPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKEHLPRLACYPPGKPAELRRRVSVDGQPFQHGDKVKCLLDSDVLREMQEGHGGWNPRMAECIGQTGTVHRVTDRGDVRVQFSHETRWTFHPGALTKHDCFWVGDVVRVIDDLDMVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVSGQLWTFSPSCLVAYRPEENTNLDVAERARENKSSLSVALDKLRAQKSGDLEQPGRLVVEVALGNLSRAVDLLRRHPEQVDTKNQGRTALQVAAYLGQVKLVRLLLQARAGTDLPDEEGNTSLHYAALGNQPEVTQVLLSVGCPADTLNSSQSTALHLAVQRGFLEVVRVLCEHGCDVNLPDSNADTPLHCAISAGTGASGIVEVLTEVPGVDVTTTNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGHCDVNVRNRKLQTPLHLAVQQAHMGLVPLLTDAGCSINAEDEEGDTALHVALQRHQLLPLAADRAGGDSGPAQLLSRLQASGLPGFAELTIGTAIACFLALEGADLSYANHRGRSPLDLAAEGRVLKALQGCAQRFRERQVGATAAGPRQLRSTPNTVTNLHVAPAAGHEAAECLVCSELALLVLFTPCQHRTVCEECARRMKKCIRCQVAISKKLRPDGSELASAAPASMPQRQLVEELQSRYRQMEERITCPICIDNHIRLVFQCGHGACAPCGAALTACPICRQPVRDRIQIFV

Target ORF information:

RefSeq Version	XM_016191015.1
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016191015.1

ORF Insert Sequence:

ATGCACCATT GCTCTTTGGT CATGACTGTG TTGCCCTGTG TTCCTTGCAG GCTCAGCTTC 
TGCCACTTTA TTCTTCAGGA CAAAGGTGCA GAGCCACTGC CCATCATGGA TCCAGACCCA 
CAGGCGGGTG TGCAGGTGGG CATGCGCGTT GTCCGTGGCG TGGACTGGAA GTGGGCCCAG 
CAGGACGGTG GCGAGGGCGG CGTGGGTACG GTGGTGGAAC TCGGCCGCCA CGGCAGCCCC 
TCCACCCCTG ACCGAACAGT GGTTGTGCAG TGGGACCATG GCACCCGCAC CAACTACCGT 
GCGGGCTATC AGGGTGCCCA TGACCTGCTG CTCTACGACA ATGCCCAGAT CGGTGTGCGG 
CACCCCAACA TCATCTGCGA CTGCTGCAAG AAACACGGGT TGCGGGGCAT GCGCTGGAAG 
TGCCTCGTCT GTTTTGACTA TGACCTGTGC ACTCAGTGTT ACATGCACAA CAGACATGAT 
CTGGCACACG CCTTTGAGCG CTACGAGACT GCACACTCGC GCCCGGTGAC GCTGAGCCCC 
CGCCAGGGCC TCCCGAGGGT TCCACTACGG GGCATCTTCC AGGGTGCGAA GGTGGTGCGG 
GGTCCTGACT GGGAGTGGGG CTCCCAGGAT GGAGGGGAGG GGAAACCAGG CCGTGTGGTG 
GACATCCGGG GCTGGGACGT GGAGACAGGC CGGAGTGTGG CCAGCGTGAC CTGGGCAGAT 
GGCACCACCA ACGTGTACCG CGTAGGCCAC AAGGGCAAGG TGGACCTCAA GTGTGTGGGC 
GAGGCAGCCG GCGGCTTCTA CTACAAGGAG CACCTCCCCA GGCTGGCCTG CTATCCCCCA 
GGCAAGCCGG CTGAACTGCG ACGCAGGGTG AGTGTGGATG GGCAGCCCTT CCAGCACGGC 
GACAAGGTCA AGTGCCTGCT GGACTCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGT 
GGCTGGAATC CCCGGATGGC GGAGTGCATT GGACAGACGG GCACCGTGCA CCGCGTCACA 
GACCGTGGGG ATGTGCGTGT GCAGTTCAGC CACGAGACCC GTTGGACCTT CCACCCAGGG 
GCTCTCACCA AGCATGACTG CTTCTGGGTG GGTGATGTGG TGCGGGTCAT CGACGACCTG 
GACATGGTGA AGCGGCTGCA GGCTGGGCAT GGCGAGTGGA CAGACGACAT GGCCCCCGCC 
CTGGGCCGTG TAGGAAAGGT GGTGAAGGTC TTTGGCGACG GGAACCTGCG TGTGGCAGTC 
AGCGGCCAGC TGTGGACCTT CAGCCCCTCA TGCCTCGTGG CCTACCGGCC CGAAGAGAAC 
ACCAACCTGG ACGTCGCTGA GCGAGCCCGG GAGAACAAGA GCTCCCTGAG TGTTGCCCTG 
GACAAACTTC GAGCCCAGAA GAGTGGTGAC CTGGAGCAAC CGGGCAGGCT GGTGGTGGAG 
GTGGCACTGG GCAACTTGTC ACGGGCTGTG GACCTGCTGC GGAGGCACCC AGAGCAGGTG 
GATACCAAGA ATCAGGGCCG GACTGCCCTG CAGGTGGCTG CCTACCTGGG CCAGGTGAAG 
CTGGTGCGGC TGCTGCTCCA GGCAAGGGCA GGCACAGACC TGCCTGATGA GGAGGGCAAC 
ACTTCACTGC ACTATGCAGC CCTAGGAAAC CAGCCGGAGG TCACCCAGGT GCTCCTGAGT 
GTGGGGTGCC CGGCTGACAC CCTCAACAGC AGCCAGAGCA CTGCACTACA CCTTGCAGTG 
CAGAGGGGCT TCCTGGAGGT GGTGCGGGTC CTGTGTGAGC ATGGCTGTGA TGTCAACCTG 
CCTGACTCGA ATGCCGACAC ACCACTGCAC TGCGCCATCT CAGCTGGCAC GGGGGCCAGT 
GGCATAGTGG AGGTCCTCAC TGAGGTGCCA GGTGTGGACG TCACCACCAC CAACAGCCAG 
GGCTTCACCC TGCTGCATCA CGCCTCCCTC AAGGGCCACA CGCTCGCTGT CAGGAGGATC 
CTGGCCCGGG CCCGGCAGCT GGTGGATGCC AAGAAGGAGG ACGGCTTCAC TGCCTTGCAC 
CTGGCCGCCC TCAACAATCA CCGAGAGGTG GCCCAGATTC TCATCCGGGA GGGCCACTGC 
GATGTGAATG TGCGTAACCG CAAGCTGCAG ACACCCCTGC ATCTGGCTGT TCAGCAGGCC 
CACATGGGGC TGGTGCCCCT GCTGACCGAC GCTGGCTGCA GCATCAATGC AGAGGATGAG 
GAGGGGGACA CAGCCCTGCA TGTGGCCCTG CAGCGGCACC AGCTGCTGCC CCTGGCAGCT 
GACCGGGCCG GGGGGGACTC TGGGCCAGCA CAGCTATTGT CCAGGCTGCA GGCCTCGGGC 
CTCCCTGGCT TTGCGGAGTT GACAATAGGC ACTGCCATCG CCTGCTTCCT CGCACTGGAG 
GGAGCCGATT TGAGCTACGC CAACCACCGT GGCCGCAGCC CGCTGGACCT GGCTGCAGAG 
GGCCGAGTGC TCAAGGCCCT GCAGGGCTGT GCCCAGCGCT TCCGGGAGCG GCAGGTGGGC 
GCCACGGCCG CCGGCCCCAG GCAGTTGCGG AGCACCCCTA ACACCGTGAC CAACCTGCAT 
GTGGCCCCAG CGGCAGGGCA TGAGGCGGCC GAGTGCCTCG TGTGCTCGGA GCTGGCGCTG 
CTGGTGCTTT TCACGCCGTG CCAGCATCGC ACTGTGTGCG AGGAGTGCGC GCGCAGGATG 
AAGAAGTGCA TCAGGTGCCA GGTGGCCATC AGCAAGAAGC TGCGCCCAGA CGGCTCGGAG 
TTGGCCAGCG CAGCCCCTGC GTCTATGCCG CAGCGGCAGC TTGTAGAGGA GCTGCAGAGC 
CGTTACCGGC AGATGGAGGA GCGCATCACC TGCCCAATCT GCATCGACAA CCACATCCGC 
CTGGTTTTCC AGTGCGGCCA CGGCGCCTGC GCGCCCTGCG GCGCTGCGCT CACTGCCTGC 
CCCATCTGCC GCCAGCCGGT CCGCGACCGA ATCCAGATCT TCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_007529486.2 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_007529548.2 Erinaceus europaeus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OEr17582
Clone ID Related Accession (Same CDS sequence)	XM_007529486.2
Accession Version	XM_007529486.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	E3 ubiquitin-protein ligase MIB2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006804353.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Apr 11, 2016 this sequence version replaced XM_007529486.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCATT GCTCTTTGGT CATGACTGTG TTGCCCTGTG TTCCTTGCAG GCTCAGCTTC 
TGCCACTTTA TTCTTCAGGA CAAAGGTGCA GAGCCACTGC CCATCATGGA TCCAGACCCA 
CAGGCGGGTG TGCAGGTGGG CATGCGCGTT GTCCGTGGCG TGGACTGGAA GTGGGCCCAG 
CAGGACGGTG GCGAGGGCGG CGTGGGTACG GTGGTGGAAC TCGGCCGCCA CGGCAGCCCC 
TCCACCCCTG ACCGAACAGT GGTTGTGCAG TGGGACCATG GCACCCGCAC CAACTACCGT 
GCGGGCTATC AGGGTGCCCA TGACCTGCTG CTCTACGACA ATGCCCAGAT CGGTGTGCGG 
CACCCCAACA TCATCTGCGA CTGCTGCAAG AAACACGGGT TGCGGGGCAT GCGCTGGAAG 
TGCCTCGTCT GTTTTGACTA TGACCTGTGC ACTCAGTGTT ACATGCACAA CAGACATGAT 
CTGGCACACG CCTTTGAGCG CTACGAGACT GCACACTCGC GCCCGGTGAC GCTGAGCCCC 
CGCCAGGGCC TCCCGAGGGT TCCACTACGG GGCATCTTCC AGGGTGCGAA GGTGGTGCGG 
GGTCCTGACT GGGAGTGGGG CTCCCAGGAT GGAGGGGAGG GGAAACCAGG CCGTGTGGTG 
GACATCCGGG GCTGGGACGT GGAGACAGGC CGGAGTGTGG CCAGCGTGAC CTGGGCAGAT 
GGCACCACCA ACGTGTACCG CGTAGGCCAC AAGGGCAAGG TGGACCTCAA GTGTGTGGGC 
GAGGCAGCCG GCGGCTTCTA CTACAAGGAG CACCTCCCCA GGCTGGGCAA GCCGGCTGAA 
CTGCGACGCA GGGTGAGTGT GGATGGGCAG CCCTTCCAGC ACGGCGACAA GGTCAAGTGC 
CTGCTGGACT CGGATGTGCT GAGGGAGATG CAGGAAGGCC ACGGTGGCTG GAATCCCCGG 
ATGGCGGAGT GCATTGGACA GACGGGCACC GTGCACCGCG TCACAGACCG TGGGGATGTG 
CGTGTGCAGT TCAGCCACGA GACCCGTTGG ACCTTCCACC CAGGGGCTCT CACCAAGCAT 
GACTGCTTCT GGGTGGGTGA TGTGGTGCGG GTCATCGACG ACCTGGACAT GGTGAAGCGG 
CTGCAGGCTG GGCATGGCGA GTGGACAGAC GACATGGCCC CCGCCCTGGG CCGTGTAGGA 
AAGGTGGTGA AGGTCTTTGG CGACGGGAAC CTGCGTGTGG CAGTCAGCGG CCAGCTGTGG 
ACCTTCAGCC CCTCATGCCT CGTGGCCTAC CGGCCCGAAG AGAACACCAA CCTGGACGTC 
GCTGAGCGAG CCCGGGAGAA CAAGAGCTCC CTGAGTGTTG CCCTGGACAA ACTTCGAGCC 
CAGAAGAGTG GTGACCTGGA GCAACCGGGC AGGCTGGTGG TGGAGGTGGC ACTGGGCAAC 
TTGTCACGGG CTGTGGACCT GCTGCGGAGG CACCCAGAGC AGGTGGATAC CAAGAATCAG 
GGCCGGACTG CCCTGCAGGT GGCTGCCTAC CTGGGCCAGG TGAAGCTGGT GCGGCTGCTG 
CTCCAGGCAA GGGCAGGCAC AGACCTGCCT GATGAGGAGG GCAACACTTC ACTGCACTAT 
GCAGCCCTAG GAAACCAGCC GGAGGTCACC CAGGTGCTCC TGAGTGTGGG GTGCCCGGCT 
GACACCCTCA ACAGCAGCCA GAGCACTGCA CTACACCTTG CAGTGCAGAG GGGCTTCCTG 
GAGGTGGTGC GGGTCCTGTG TGAGCATGGC TGTGATGTCA ACCTGCCTGA CTCGAATGCC 
GACACACCAC TGCACTGCGC CATCTCAGCT GGCACGGGGG CCAGTGGCAT AGTGGAGGTC 
CTCACTGAGG TGCCAGGTGT GGACGTCACC ACCACCAACA GCCAGGGCTT CACCCTGCTG 
CATCACGCCT CCCTCAAGGG CCACACGCTC GCTGTCAGGA GGATCCTGGC CCGGGCCCGG 
CAGCTGGTGG ATGCCAAGAA GGAGGACGGC TTCACTGCCT TGCACCTGGC CGCCCTCAAC 
AATCACCGAG AGGTGGCCCA GATTCTCATC CGGGAGGGCC ACTGCGATGT GAATGTGCGT 
AACCGCAAGC TGCAGACACC CCTGCATCTG GCTGTTCAGC AGGCCCACAT GGGGCTGGTG 
CCCCTGCTGA CCGACGCTGG CTGCAGCATC AATGCAGAGG ATGAGGAGGG GGACACAGCC 
CTGCATGTGG CCCTGCAGCG GCACCAGCTG CTGCCCCTGG CAGCTGACCG GGCCGGGGGG 
GACTCTGGGC CAGCACAGCT ATTGTCCAGG CTGCAGGCCT CGGGCCTCCC TGGCTTTGCG 
GAGTTGACAA TAGGCACTGC CATCGCCTGC TTCCTCGCAC TGGAGGGAGC CGATTTGAGC 
TACGCCAACC ACCGTGGCCG CAGCCCGCTG GACCTGGCTG CAGAGGGCCG AGTGCTCAAG 
GCCCTGCAGG GCTGTGCCCA GCGCTTCCGG GAGCGGCAGG TGGGCGCCAC GGCCGCCGGC 
CCCAGGCAGT TGCGGAGCAC CCCTAACACC GTGACCAACC TGCATGTGGC CCCAGCGGCA 
GGGCATGAGG CGGCCGAGTG CCTCGTGTGC TCGGAGCTGG CGCTGCTGGT GCTTTTCACG 
CCGTGCCAGC ATCGCACTGT GTGCGAGGAG TGCGCGCGCA GGATGAAGAA GTGCATCAGG 
TGCCAGGTGG CCATCAGCAA GAAGCTGCGC CCAGACGGCT CGGAGTTGGC CAGCGCAGCC 
CCTGCGTCTA TGCCGCAGCG GCAGCTTGTA GAGGAGCTGC AGAGCCGTTA CCGGCAGATG 
GAGGAGCGCA TCACCTGCCC AATCTGCATC GACAACCACA TCCGCCTGGT TTTCCAGTGC 
GGCCACGGCG CCTGCGCGCC CTGCGGCGCT GCGCTCACTG CCTGCCCCAT CTGCCGCCAG 
CCGGTCCGCG ACCGAATCCA GATCTTCGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007529548.2
CDS	419..3391
Translation	MHHCSLVMTVLPCVPCRLSFCHFILQDKGAEPLPIMDPDPQAGVQVGMRVVRGVDWKWAQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCLVCFDYDLCTQCYMHNRHDLAHAFERYETAHSRPVTLSPRQGLPRVPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKEHLPRLGKPAELRRRVSVDGQPFQHGDKVKCLLDSDVLREMQEGHGGWNPRMAECIGQTGTVHRVTDRGDVRVQFSHETRWTFHPGALTKHDCFWVGDVVRVIDDLDMVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVSGQLWTFSPSCLVAYRPEENTNLDVAERARENKSSLSVALDKLRAQKSGDLEQPGRLVVEVALGNLSRAVDLLRRHPEQVDTKNQGRTALQVAAYLGQVKLVRLLLQARAGTDLPDEEGNTSLHYAALGNQPEVTQVLLSVGCPADTLNSSQSTALHLAVQRGFLEVVRVLCEHGCDVNLPDSNADTPLHCAISAGTGASGIVEVLTEVPGVDVTTTNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGHCDVNVRNRKLQTPLHLAVQQAHMGLVPLLTDAGCSINAEDEEGDTALHVALQRHQLLPLAADRAGGDSGPAQLLSRLQASGLPGFAELTIGTAIACFLALEGADLSYANHRGRSPLDLAAEGRVLKALQGCAQRFRERQVGATAAGPRQLRSTPNTVTNLHVAPAAGHEAAECLVCSELALLVLFTPCQHRTVCEECARRMKKCIRCQVAISKKLRPDGSELASAAPASMPQRQLVEELQSRYRQMEERITCPICIDNHIRLVFQCGHGACAPCGAALTACPICRQPVRDRIQIFV

Target ORF information:

RefSeq Version	XM_007529486.2
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007529486.2

ORF Insert Sequence:

ATGCACCATT GCTCTTTGGT CATGACTGTG TTGCCCTGTG TTCCTTGCAG GCTCAGCTTC 
TGCCACTTTA TTCTTCAGGA CAAAGGTGCA GAGCCACTGC CCATCATGGA TCCAGACCCA 
CAGGCGGGTG TGCAGGTGGG CATGCGCGTT GTCCGTGGCG TGGACTGGAA GTGGGCCCAG 
CAGGACGGTG GCGAGGGCGG CGTGGGTACG GTGGTGGAAC TCGGCCGCCA CGGCAGCCCC 
TCCACCCCTG ACCGAACAGT GGTTGTGCAG TGGGACCATG GCACCCGCAC CAACTACCGT 
GCGGGCTATC AGGGTGCCCA TGACCTGCTG CTCTACGACA ATGCCCAGAT CGGTGTGCGG 
CACCCCAACA TCATCTGCGA CTGCTGCAAG AAACACGGGT TGCGGGGCAT GCGCTGGAAG 
TGCCTCGTCT GTTTTGACTA TGACCTGTGC ACTCAGTGTT ACATGCACAA CAGACATGAT 
CTGGCACACG CCTTTGAGCG CTACGAGACT GCACACTCGC GCCCGGTGAC GCTGAGCCCC 
CGCCAGGGCC TCCCGAGGGT TCCACTACGG GGCATCTTCC AGGGTGCGAA GGTGGTGCGG 
GGTCCTGACT GGGAGTGGGG CTCCCAGGAT GGAGGGGAGG GGAAACCAGG CCGTGTGGTG 
GACATCCGGG GCTGGGACGT GGAGACAGGC CGGAGTGTGG CCAGCGTGAC CTGGGCAGAT 
GGCACCACCA ACGTGTACCG CGTAGGCCAC AAGGGCAAGG TGGACCTCAA GTGTGTGGGC 
GAGGCAGCCG GCGGCTTCTA CTACAAGGAG CACCTCCCCA GGCTGGGCAA GCCGGCTGAA 
CTGCGACGCA GGGTGAGTGT GGATGGGCAG CCCTTCCAGC ACGGCGACAA GGTCAAGTGC 
CTGCTGGACT CGGATGTGCT GAGGGAGATG CAGGAAGGCC ACGGTGGCTG GAATCCCCGG 
ATGGCGGAGT GCATTGGACA GACGGGCACC GTGCACCGCG TCACAGACCG TGGGGATGTG 
CGTGTGCAGT TCAGCCACGA GACCCGTTGG ACCTTCCACC CAGGGGCTCT CACCAAGCAT 
GACTGCTTCT GGGTGGGTGA TGTGGTGCGG GTCATCGACG ACCTGGACAT GGTGAAGCGG 
CTGCAGGCTG GGCATGGCGA GTGGACAGAC GACATGGCCC CCGCCCTGGG CCGTGTAGGA 
AAGGTGGTGA AGGTCTTTGG CGACGGGAAC CTGCGTGTGG CAGTCAGCGG CCAGCTGTGG 
ACCTTCAGCC CCTCATGCCT CGTGGCCTAC CGGCCCGAAG AGAACACCAA CCTGGACGTC 
GCTGAGCGAG CCCGGGAGAA CAAGAGCTCC CTGAGTGTTG CCCTGGACAA ACTTCGAGCC 
CAGAAGAGTG GTGACCTGGA GCAACCGGGC AGGCTGGTGG TGGAGGTGGC ACTGGGCAAC 
TTGTCACGGG CTGTGGACCT GCTGCGGAGG CACCCAGAGC AGGTGGATAC CAAGAATCAG 
GGCCGGACTG CCCTGCAGGT GGCTGCCTAC CTGGGCCAGG TGAAGCTGGT GCGGCTGCTG 
CTCCAGGCAA GGGCAGGCAC AGACCTGCCT GATGAGGAGG GCAACACTTC ACTGCACTAT 
GCAGCCCTAG GAAACCAGCC GGAGGTCACC CAGGTGCTCC TGAGTGTGGG GTGCCCGGCT 
GACACCCTCA ACAGCAGCCA GAGCACTGCA CTACACCTTG CAGTGCAGAG GGGCTTCCTG 
GAGGTGGTGC GGGTCCTGTG TGAGCATGGC TGTGATGTCA ACCTGCCTGA CTCGAATGCC 
GACACACCAC TGCACTGCGC CATCTCAGCT GGCACGGGGG CCAGTGGCAT AGTGGAGGTC 
CTCACTGAGG TGCCAGGTGT GGACGTCACC ACCACCAACA GCCAGGGCTT CACCCTGCTG 
CATCACGCCT CCCTCAAGGG CCACACGCTC GCTGTCAGGA GGATCCTGGC CCGGGCCCGG 
CAGCTGGTGG ATGCCAAGAA GGAGGACGGC TTCACTGCCT TGCACCTGGC CGCCCTCAAC 
AATCACCGAG AGGTGGCCCA GATTCTCATC CGGGAGGGCC ACTGCGATGT GAATGTGCGT 
AACCGCAAGC TGCAGACACC CCTGCATCTG GCTGTTCAGC AGGCCCACAT GGGGCTGGTG 
CCCCTGCTGA CCGACGCTGG CTGCAGCATC AATGCAGAGG ATGAGGAGGG GGACACAGCC 
CTGCATGTGG CCCTGCAGCG GCACCAGCTG CTGCCCCTGG CAGCTGACCG GGCCGGGGGG 
GACTCTGGGC CAGCACAGCT ATTGTCCAGG CTGCAGGCCT CGGGCCTCCC TGGCTTTGCG 
GAGTTGACAA TAGGCACTGC CATCGCCTGC TTCCTCGCAC TGGAGGGAGC CGATTTGAGC 
TACGCCAACC ACCGTGGCCG CAGCCCGCTG GACCTGGCTG CAGAGGGCCG AGTGCTCAAG 
GCCCTGCAGG GCTGTGCCCA GCGCTTCCGG GAGCGGCAGG TGGGCGCCAC GGCCGCCGGC 
CCCAGGCAGT TGCGGAGCAC CCCTAACACC GTGACCAACC TGCATGTGGC CCCAGCGGCA 
GGGCATGAGG CGGCCGAGTG CCTCGTGTGC TCGGAGCTGG CGCTGCTGGT GCTTTTCACG 
CCGTGCCAGC ATCGCACTGT GTGCGAGGAG TGCGCGCGCA GGATGAAGAA GTGCATCAGG 
TGCCAGGTGG CCATCAGCAA GAAGCTGCGC CCAGACGGCT CGGAGTTGGC CAGCGCAGCC 
CCTGCGTCTA TGCCGCAGCG GCAGCTTGTA GAGGAGCTGC AGAGCCGTTA CCGGCAGATG 
GAGGAGCGCA TCACCTGCCC AATCTGCATC GACAACCACA TCCGCCTGGT TTTCCAGTGC 
GGCCACGGCG CCTGCGCGCC CTGCGGCGCT GCGCTCACTG CCTGCCCCAT CTGCCGCCAG 
CCGGTCCGCG ACCGAATCCA GATCTTCGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_007529490.2 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_007529552.2 Erinaceus europaeus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X3, mRNA.

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CloneID	OEr17583
Clone ID Related Accession (Same CDS sequence)	XM_007529490.2
Accession Version	XM_007529490.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	E3 ubiquitin-protein ligase MIB2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006804353.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Apr 11, 2016 this sequence version replaced XM_007529490.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCC ACAGTTCCCT GCCCCAGGAT ATTGGCACCT CGCCCCCTCC TGGTGGGCTG 
AGAGGGTGCT GTGCCCACAG GTGCAGAGCC ACTGCCCATC ATGGATCCAG ACCCACAGGC 
GGGTGTGCAG GTGTGCGGCA CCCCAACATC ATCTGCGACT GCTGCAAGAA ACACGGGTTG 
CGGGGCATGC GCTGGAAGTG CCTCGTCTGT TTTGACTATG ACCTGTGCAC TCAGTGTTAC 
ATGCACAACA GACATGATCT GGCACACGCC TTTGAGCGCT ACGAGACTGC ACACTCGCGC 
CCGGTGACGC TGAGCCCCCG CCAGGGCCTC CCGAGGGTTC CACTACGGGG CATCTTCCAG 
GGTGCGAAGG TGGTGCGGGG TCCTGACTGG GAGTGGGGCT CCCAGGATGG AGGGGAGGGG 
AAACCAGGCC GTGTGGTGGA CATCCGGGGC TGGGACGTGG AGACAGGCCG GAGTGTGGCC 
AGCGTGACCT GGGCAGATGG CACCACCAAC GTGTACCGCG TAGGCCACAA GGGCAAGGTG 
GACCTCAAGT GTGTGGGCGA GGCAGCCGGC GGCTTCTACT ACAAGGAGCA CCTCCCCAGG 
CTGGGCAAGC CGGCTGAACT GCGACGCAGG GTGAGTGTGG ATGGGCAGCC CTTCCAGCAC 
GGCGACAAGG TCAAGTGCCT GCTGGACTCG GATGTGCTGA GGGAGATGCA GGAAGGCCAC 
GGTGGCTGGA ATCCCCGGAT GGCGGAGTGC ATTGGACAGA CGGGCACCGT GCACCGCGTC 
ACAGACCGTG GGGATGTGCG TGTGCAGTTC AGCCACGAGA CCCGTTGGAC CTTCCACCCA 
GGGGCTCTCA CCAAGCATGA CTGCTTCTGG GTGGGTGATG TGGTGCGGGT CATCGACGAC 
CTGGACATGG TGAAGCGGCT GCAGGCTGGG CATGGCGAGT GGACAGACGA CATGGCCCCC 
GCCCTGGGCC GTGTAGGAAA GGTGGTGAAG GTCTTTGGCG ACGGGAACCT GCGTGTGGCA 
GTCAGCGGCC AGCTGTGGAC CTTCAGCCCC TCATGCCTCG TGGCCTACCG GCCCGAAGAG 
AACACCAACC TGGACGTCGC TGAGCGAGCC CGGGAGAACA AGAGCTCCCT GAGTGTTGCC 
CTGGACAAAC TTCGAGCCCA GAAGAGTGGT GACCTGGAGC AACCGGGCAG GCTGGTGGTG 
GAGGTGGCAC TGGGCAACTT GTCACGGGCT GTGGACCTGC TGCGGAGGCA CCCAGAGCAG 
GTGGATACCA AGAATCAGGG CCGGACTGCC CTGCAGGTGG CTGCCTACCT GGGCCAGGTG 
AAGCTGGTGC GGCTGCTGCT CCAGGCAAGG GCAGGCACAG ACCTGCCTGA TGAGGAGGGC 
AACACTTCAC TGCACTATGC AGCCCTAGGA AACCAGCCGG AGGTCACCCA GGTGCTCCTG 
AGTGTGGGGT GCCCGGCTGA CACCCTCAAC AGCAGCCAGA GCACTGCACT ACACCTTGCA 
GTGCAGAGGG GCTTCCTGGA GGTGGTGCGG GTCCTGTGTG AGCATGGCTG TGATGTCAAC 
CTGCCTGACT CGAATGCCGA CACACCACTG CACTGCGCCA TCTCAGCTGG CACGGGGGCC 
AGTGGCATAG TGGAGGTCCT CACTGAGGTG CCAGGTGTGG ACGTCACCAC CACCAACAGC 
CAGGGCTTCA CCCTGCTGCA TCACGCCTCC CTCAAGGGCC ACACGCTCGC TGTCAGGAGG 
ATCCTGGCCC GGGCCCGGCA GCTGGTGGAT GCCAAGAAGG AGGACGGCTT CACTGCCTTG 
CACCTGGCCG CCCTCAACAA TCACCGAGAG GTGGCCCAGA TTCTCATCCG GGAGGGCCAC 
TGCGATGTGA ATGTGCGTAA CCGCAAGCTG CAGACACCCC TGCATCTGGC TGTTCAGCAG 
GCCCACATGG GGCTGGTGCC CCTGCTGACC GACGCTGGCT GCAGCATCAA TGCAGAGGAT 
GAGGAGGGGG ACACAGCCCT GCATGTGGCC CTGCAGCGGC ACCAGCTGCT GCCCCTGGCA 
GCTGACCGGG CCGGGGGGGA CTCTGGGCCA GCACAGCTAT TGTCCAGGCT GCAGGCCTCG 
GGCCTCCCTG GCTTTGCGGA GTTGACAATA GGCACTGCCA TCGCCTGCTT CCTCGCACTG 
GAGGGAGCCG ATTTGAGCTA CGCCAACCAC CGTGGCCGCA GCCCGCTGGA CCTGGCTGCA 
GAGGGCCGAG TGCTCAAGGC CCTGCAGGGC TGTGCCCAGC GCTTCCGGGA GCGGCAGGTG 
GGCGCCACGG CCGCCGGCCC CAGGCAGTTG CGGAGCACCC CTAACACCGT GACCAACCTG 
CATGTGGCCC CAGCGGCAGG GCATGAGGCG GCCGAGTGCC TCGTGTGCTC GGAGCTGGCG 
CTGCTGGTGC TTTTCACGCC GTGCCAGCAT CGCACTGTGT GCGAGGAGTG CGCGCGCAGG 
ATGAAGAAGT GCATCAGGTG CCAGGTGGCC ATCAGCAAGA AGCTGCGCCC AGACGGCTCG 
GAGTTGGCCA GCGCAGCCCC TGCGTCTATG CCGCAGCGGC AGCTTGTAGA GGAGCTGCAG 
AGCCGTTACC GGCAGATGGA GGAGCGCATC ACCTGCCCAA TCTGCATCGA CAACCACATC 
CGCCTGGTTT TCCAGTGCGG CCACGGCGCC TGCGCGCCCT GCGGCGCTGC GCTCACTGCC 
TGCCCCATCT GCCGCCAGCC GGTCCGCGAC CGAATCCAGA TCTTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007529552.2
CDS	306..3056
Translation	MMAHSSLPQDIGTSPPPGGLRGCCAHRCRATAHHGSRPTGGCAGVRHPNIICDCCKKHGLRGMRWKCLVCFDYDLCTQCYMHNRHDLAHAFERYETAHSRPVTLSPRQGLPRVPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKEHLPRLGKPAELRRRVSVDGQPFQHGDKVKCLLDSDVLREMQEGHGGWNPRMAECIGQTGTVHRVTDRGDVRVQFSHETRWTFHPGALTKHDCFWVGDVVRVIDDLDMVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVSGQLWTFSPSCLVAYRPEENTNLDVAERARENKSSLSVALDKLRAQKSGDLEQPGRLVVEVALGNLSRAVDLLRRHPEQVDTKNQGRTALQVAAYLGQVKLVRLLLQARAGTDLPDEEGNTSLHYAALGNQPEVTQVLLSVGCPADTLNSSQSTALHLAVQRGFLEVVRVLCEHGCDVNLPDSNADTPLHCAISAGTGASGIVEVLTEVPGVDVTTTNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGHCDVNVRNRKLQTPLHLAVQQAHMGLVPLLTDAGCSINAEDEEGDTALHVALQRHQLLPLAADRAGGDSGPAQLLSRLQASGLPGFAELTIGTAIACFLALEGADLSYANHRGRSPLDLAAEGRVLKALQGCAQRFRERQVGATAAGPRQLRSTPNTVTNLHVAPAAGHEAAECLVCSELALLVLFTPCQHRTVCEECARRMKKCIRCQVAISKKLRPDGSELASAAPASMPQRQLVEELQSRYRQMEERITCPICIDNHIRLVFQCGHGACAPCGAALTACPICRQPVRDRIQIFV

Target ORF information:

RefSeq Version	XM_007529490.2
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007529490.2

ORF Insert Sequence:

ATGATGGCCC ACAGTTCCCT GCCCCAGGAT ATTGGCACCT CGCCCCCTCC TGGTGGGCTG 
AGAGGGTGCT GTGCCCACAG GTGCAGAGCC ACTGCCCATC ATGGATCCAG ACCCACAGGC 
GGGTGTGCAG GTGTGCGGCA CCCCAACATC ATCTGCGACT GCTGCAAGAA ACACGGGTTG 
CGGGGCATGC GCTGGAAGTG CCTCGTCTGT TTTGACTATG ACCTGTGCAC TCAGTGTTAC 
ATGCACAACA GACATGATCT GGCACACGCC TTTGAGCGCT ACGAGACTGC ACACTCGCGC 
CCGGTGACGC TGAGCCCCCG CCAGGGCCTC CCGAGGGTTC CACTACGGGG CATCTTCCAG 
GGTGCGAAGG TGGTGCGGGG TCCTGACTGG GAGTGGGGCT CCCAGGATGG AGGGGAGGGG 
AAACCAGGCC GTGTGGTGGA CATCCGGGGC TGGGACGTGG AGACAGGCCG GAGTGTGGCC 
AGCGTGACCT GGGCAGATGG CACCACCAAC GTGTACCGCG TAGGCCACAA GGGCAAGGTG 
GACCTCAAGT GTGTGGGCGA GGCAGCCGGC GGCTTCTACT ACAAGGAGCA CCTCCCCAGG 
CTGGGCAAGC CGGCTGAACT GCGACGCAGG GTGAGTGTGG ATGGGCAGCC CTTCCAGCAC 
GGCGACAAGG TCAAGTGCCT GCTGGACTCG GATGTGCTGA GGGAGATGCA GGAAGGCCAC 
GGTGGCTGGA ATCCCCGGAT GGCGGAGTGC ATTGGACAGA CGGGCACCGT GCACCGCGTC 
ACAGACCGTG GGGATGTGCG TGTGCAGTTC AGCCACGAGA CCCGTTGGAC CTTCCACCCA 
GGGGCTCTCA CCAAGCATGA CTGCTTCTGG GTGGGTGATG TGGTGCGGGT CATCGACGAC 
CTGGACATGG TGAAGCGGCT GCAGGCTGGG CATGGCGAGT GGACAGACGA CATGGCCCCC 
GCCCTGGGCC GTGTAGGAAA GGTGGTGAAG GTCTTTGGCG ACGGGAACCT GCGTGTGGCA 
GTCAGCGGCC AGCTGTGGAC CTTCAGCCCC TCATGCCTCG TGGCCTACCG GCCCGAAGAG 
AACACCAACC TGGACGTCGC TGAGCGAGCC CGGGAGAACA AGAGCTCCCT GAGTGTTGCC 
CTGGACAAAC TTCGAGCCCA GAAGAGTGGT GACCTGGAGC AACCGGGCAG GCTGGTGGTG 
GAGGTGGCAC TGGGCAACTT GTCACGGGCT GTGGACCTGC TGCGGAGGCA CCCAGAGCAG 
GTGGATACCA AGAATCAGGG CCGGACTGCC CTGCAGGTGG CTGCCTACCT GGGCCAGGTG 
AAGCTGGTGC GGCTGCTGCT CCAGGCAAGG GCAGGCACAG ACCTGCCTGA TGAGGAGGGC 
AACACTTCAC TGCACTATGC AGCCCTAGGA AACCAGCCGG AGGTCACCCA GGTGCTCCTG 
AGTGTGGGGT GCCCGGCTGA CACCCTCAAC AGCAGCCAGA GCACTGCACT ACACCTTGCA 
GTGCAGAGGG GCTTCCTGGA GGTGGTGCGG GTCCTGTGTG AGCATGGCTG TGATGTCAAC 
CTGCCTGACT CGAATGCCGA CACACCACTG CACTGCGCCA TCTCAGCTGG CACGGGGGCC 
AGTGGCATAG TGGAGGTCCT CACTGAGGTG CCAGGTGTGG ACGTCACCAC CACCAACAGC 
CAGGGCTTCA CCCTGCTGCA TCACGCCTCC CTCAAGGGCC ACACGCTCGC TGTCAGGAGG 
ATCCTGGCCC GGGCCCGGCA GCTGGTGGAT GCCAAGAAGG AGGACGGCTT CACTGCCTTG 
CACCTGGCCG CCCTCAACAA TCACCGAGAG GTGGCCCAGA TTCTCATCCG GGAGGGCCAC 
TGCGATGTGA ATGTGCGTAA CCGCAAGCTG CAGACACCCC TGCATCTGGC TGTTCAGCAG 
GCCCACATGG GGCTGGTGCC CCTGCTGACC GACGCTGGCT GCAGCATCAA TGCAGAGGAT 
GAGGAGGGGG ACACAGCCCT GCATGTGGCC CTGCAGCGGC ACCAGCTGCT GCCCCTGGCA 
GCTGACCGGG CCGGGGGGGA CTCTGGGCCA GCACAGCTAT TGTCCAGGCT GCAGGCCTCG 
GGCCTCCCTG GCTTTGCGGA GTTGACAATA GGCACTGCCA TCGCCTGCTT CCTCGCACTG 
GAGGGAGCCG ATTTGAGCTA CGCCAACCAC CGTGGCCGCA GCCCGCTGGA CCTGGCTGCA 
GAGGGCCGAG TGCTCAAGGC CCTGCAGGGC TGTGCCCAGC GCTTCCGGGA GCGGCAGGTG 
GGCGCCACGG CCGCCGGCCC CAGGCAGTTG CGGAGCACCC CTAACACCGT GACCAACCTG 
CATGTGGCCC CAGCGGCAGG GCATGAGGCG GCCGAGTGCC TCGTGTGCTC GGAGCTGGCG 
CTGCTGGTGC TTTTCACGCC GTGCCAGCAT CGCACTGTGT GCGAGGAGTG CGCGCGCAGG 
ATGAAGAAGT GCATCAGGTG CCAGGTGGCC ATCAGCAAGA AGCTGCGCCC AGACGGCTCG 
GAGTTGGCCA GCGCAGCCCC TGCGTCTATG CCGCAGCGGC AGCTTGTAGA GGAGCTGCAG 
AGCCGTTACC GGCAGATGGA GGAGCGCATC ACCTGCCCAA TCTGCATCGA CAACCACATC 
CGCCTGGTTT TCCAGTGCGG CCACGGCGCC TGCGCGCCCT GCGGCGCTGC GCTCACTGCC 
TGCCCCATCT GCCGCCAGCC GGTCCGCGAC CGAATCCAGA TCTTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_016191016.1 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_016046502.1 Erinaceus europaeus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OEr17584
Clone ID Related Accession (Same CDS sequence)	XM_016191016.1
Accession Version	XM_016191016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	E3 ubiquitin-protein ligase MIB2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006804353.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCAG ACCCACAGGC GGGTGTGCAG GTGGGCATGC GCGTTGTCCG TGGCGTGGAC 
TGGAAGTGGG CCCAGCAGGA CGGTGGCGAG GGCGGCGTGG GTACGGTGGT GGAACTCGGC 
CGCCACGGCA GCCCCTCCAC CCCTGACCGA ACAGTGGTTG TGCAGTGGGA CCATGGCACC 
CGCACCAACT ACCGTGCGGG CTATCAGGGT GCCCATGACC TGCTGCTCTA CGACAATGCC 
CAGATCGGTG TGCGGCACCC CAACATCATC TGCGACTGCT GCAAGAAACA CGGGTTGCGG 
GGCATGCGCT GGAAGTGCCT CGTCTGTTTT GACTATGACC TGTGCACTCA GTGTTACATG 
CACAACAGAC ATGATCTGGC ACACGCCTTT GAGCGCTACG AGACTGCACA CTCGCGCCCG 
GTGACGCTGA GCCCCCGCCA GGGCCTCCCG AGGGTTCCAC TACGGGGCAT CTTCCAGGGT 
GCGAAGGTGG TGCGGGGTCC TGACTGGGAG TGGGGCTCCC AGGATGGAGG GGAGGGGAAA 
CCAGGCCGTG TGGTGGACAT CCGGGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGC 
GTGACCTGGG CAGATGGCAC CACCAACGTG TACCGCGTAG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCCGGCGGC TTCTACTACA AGGAGCACCT CCCCAGGCTG 
GCCTGCTATC CCCCAGGCAA GCCGGCTGAA CTGCGACGCA GGGTGAGTGT GGATGGGCAG 
CCCTTCCAGC ACGGCGACAA GGTCAAGTGC CTGCTGGACT CGGATGTGCT GAGGGAGATG 
CAGGAAGGCC ACGGTGGCTG GAATCCCCGG ATGGCGGAGT GCATTGGACA GACGGGCACC 
GTGCACCGCG TCACAGACCG TGGGGATGTG CGTGTGCAGT TCAGCCACGA GACCCGTTGG 
ACCTTCCACC CAGGGGCTCT CACCAAGCAT GACTGCTTCT GGGTGGGTGA TGTGGTGCGG 
GTCATCGACG ACCTGGACAT GGTGAAGCGG CTGCAGGCTG GGCATGGCGA GTGGACAGAC 
GACATGGCCC CCGCCCTGGG CCGTGTAGGA AAGGTGGTGA AGGTCTTTGG CGACGGGAAC 
CTGCGTGTGG CAGTCAGCGG CCAGCTGTGG ACCTTCAGCC CCTCATGCCT CGTGGCCTAC 
CGGCCCGAAG AGAACACCAA CCTGGACGTC GCTGAGCGAG CCCGGGAGAA CAAGAGCTCC 
CTGAGTGTTG CCCTGGACAA ACTTCGAGCC CAGAAGAGTG GTGACCTGGA GCAACCGGGC 
AGGCTGGTGG TGGAGGTGGC ACTGGGCAAC TTGTCACGGG CTGTGGACCT GCTGCGGAGG 
CACCCAGAGC AGGTGGATAC CAAGAATCAG GGCCGGACTG CCCTGCAGGT GGCTGCCTAC 
CTGGGCCAGG TGAAGCTGGT GCGGCTGCTG CTCCAGGCAA GGGCAGGCAC AGACCTGCCT 
GATGAGGAGG GCAACACTTC ACTGCACTAT GCAGCCCTAG GAAACCAGCC GGAGGTCACC 
CAGGTGCTCC TGAGTGTGGG GTGCCCGGCT GACACCCTCA ACAGCAGCCA GAGCACTGCA 
CTACACCTTG CAGTGCAGAG GGGCTTCCTG GAGGTGGTGC GGGTCCTGTG TGAGCATGGC 
TGTGATGTCA ACCTGCCTGA CTCGAATGCC GACACACCAC TGCACTGCGC CATCTCAGCT 
GGCACGGGGG CCAGTGGCAT AGTGGAGGTC CTCACTGAGG TGCCAGGTGT GGACGTCACC 
ACCACCAACA GCCAGGGCTT CACCCTGCTG CATCACGCCT CCCTCAAGGG CCACACGCTC 
GCTGTCAGGA GGATCCTGGC CCGGGCCCGG CAGCTGGTGG ATGCCAAGAA GGAGGACGGC 
TTCACTGCCT TGCACCTGGC CGCCCTCAAC AATCACCGAG AGGTGGCCCA GATTCTCATC 
CGGGAGGGCC ACTGCGATGT GAATGTGCGT AACCGCAAGC TGCAGACACC CCTGCATCTG 
GCTGTTCAGC AGGCCCACAT GGGGCTGGTG CCCCTGCTGA CCGACGCTGG CTGCAGCATC 
AATGCAGAGG ATGAGGAGGG GGACACAGCC CTGCATGTGG CCCTGCAGCG GCACCAGCTG 
CTGCCCCTGG CAGCTGACCG GGCCGGGGGG GACTCTGGGC CAGCACAGCT ATTGTCCAGG 
CTGCAGGCCT CGGGCCTCCC TGGCTTTGCG GAGTTGACAA TAGGCACTGC CATCGCCTGC 
TTCCTCGCAC TGGAGGGAGC CGATTTGAGC TACGCCAACC ACCGTGGCCG CAGCCCGCTG 
GACCTGGCTG CAGAGGGCCG AGTGCTCAAG GCCCTGCAGG GCTGTGCCCA GCGCTTCCGG 
GAGCGGCAGG TGGGCGCCAC GGCCGCCGGC CCCAGGCAGT TGCGGAGCAC CCCTAACACC 
GTGACCAACC TGCATGTGGC CCCAGCGGCA GGGCATGAGG CGGCCGAGTG CCTCGTGTGC 
TCGGAGCTGG CGCTGCTGGT GCTTTTCACG CCGTGCCAGC ATCGCACTGT GTGCGAGGAG 
TGCGCGCGCA GGATGAAGAA GTGCATCAGG TGCCAGGTGG CCATCAGCAA GAAGCTGCGC 
CCAGACGGCT CGGAGTTGGC CAGCGCAGCC CCTGCGTCTA TGCCGCAGCG GCAGCTTGTA 
GAGGAGCTGC AGAGCCGTTA CCGGCAGATG GAGGAGCGCA TCACCTGCCC AATCTGCATC 
GACAACCACA TCCGCCTGGT TTTCCAGTGC GGCCACGGCG CCTGCGCGCC CTGCGGCGCT 
GCGCTCACTG CCTGCCCCAT CTGCCGCCAG CCGGTCCGCG ACCGAATCCA GATCTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016046502.1
CDS	293..3175
Translation	MDPDPQAGVQVGMRVVRGVDWKWAQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCLVCFDYDLCTQCYMHNRHDLAHAFERYETAHSRPVTLSPRQGLPRVPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKEHLPRLACYPPGKPAELRRRVSVDGQPFQHGDKVKCLLDSDVLREMQEGHGGWNPRMAECIGQTGTVHRVTDRGDVRVQFSHETRWTFHPGALTKHDCFWVGDVVRVIDDLDMVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVSGQLWTFSPSCLVAYRPEENTNLDVAERARENKSSLSVALDKLRAQKSGDLEQPGRLVVEVALGNLSRAVDLLRRHPEQVDTKNQGRTALQVAAYLGQVKLVRLLLQARAGTDLPDEEGNTSLHYAALGNQPEVTQVLLSVGCPADTLNSSQSTALHLAVQRGFLEVVRVLCEHGCDVNLPDSNADTPLHCAISAGTGASGIVEVLTEVPGVDVTTTNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGHCDVNVRNRKLQTPLHLAVQQAHMGLVPLLTDAGCSINAEDEEGDTALHVALQRHQLLPLAADRAGGDSGPAQLLSRLQASGLPGFAELTIGTAIACFLALEGADLSYANHRGRSPLDLAAEGRVLKALQGCAQRFRERQVGATAAGPRQLRSTPNTVTNLHVAPAAGHEAAECLVCSELALLVLFTPCQHRTVCEECARRMKKCIRCQVAISKKLRPDGSELASAAPASMPQRQLVEELQSRYRQMEERITCPICIDNHIRLVFQCGHGACAPCGAALTACPICRQPVRDRIQIFV

Target ORF information:

RefSeq Version	XM_016191016.1
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016191016.1

ORF Insert Sequence:

ATGGATCCAG ACCCACAGGC GGGTGTGCAG GTGGGCATGC GCGTTGTCCG TGGCGTGGAC 
TGGAAGTGGG CCCAGCAGGA CGGTGGCGAG GGCGGCGTGG GTACGGTGGT GGAACTCGGC 
CGCCACGGCA GCCCCTCCAC CCCTGACCGA ACAGTGGTTG TGCAGTGGGA CCATGGCACC 
CGCACCAACT ACCGTGCGGG CTATCAGGGT GCCCATGACC TGCTGCTCTA CGACAATGCC 
CAGATCGGTG TGCGGCACCC CAACATCATC TGCGACTGCT GCAAGAAACA CGGGTTGCGG 
GGCATGCGCT GGAAGTGCCT CGTCTGTTTT GACTATGACC TGTGCACTCA GTGTTACATG 
CACAACAGAC ATGATCTGGC ACACGCCTTT GAGCGCTACG AGACTGCACA CTCGCGCCCG 
GTGACGCTGA GCCCCCGCCA GGGCCTCCCG AGGGTTCCAC TACGGGGCAT CTTCCAGGGT 
GCGAAGGTGG TGCGGGGTCC TGACTGGGAG TGGGGCTCCC AGGATGGAGG GGAGGGGAAA 
CCAGGCCGTG TGGTGGACAT CCGGGGCTGG GACGTGGAGA CAGGCCGGAG TGTGGCCAGC 
GTGACCTGGG CAGATGGCAC CACCAACGTG TACCGCGTAG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCCGGCGGC TTCTACTACA AGGAGCACCT CCCCAGGCTG 
GCCTGCTATC CCCCAGGCAA GCCGGCTGAA CTGCGACGCA GGGTGAGTGT GGATGGGCAG 
CCCTTCCAGC ACGGCGACAA GGTCAAGTGC CTGCTGGACT CGGATGTGCT GAGGGAGATG 
CAGGAAGGCC ACGGTGGCTG GAATCCCCGG ATGGCGGAGT GCATTGGACA GACGGGCACC 
GTGCACCGCG TCACAGACCG TGGGGATGTG CGTGTGCAGT TCAGCCACGA GACCCGTTGG 
ACCTTCCACC CAGGGGCTCT CACCAAGCAT GACTGCTTCT GGGTGGGTGA TGTGGTGCGG 
GTCATCGACG ACCTGGACAT GGTGAAGCGG CTGCAGGCTG GGCATGGCGA GTGGACAGAC 
GACATGGCCC CCGCCCTGGG CCGTGTAGGA AAGGTGGTGA AGGTCTTTGG CGACGGGAAC 
CTGCGTGTGG CAGTCAGCGG CCAGCTGTGG ACCTTCAGCC CCTCATGCCT CGTGGCCTAC 
CGGCCCGAAG AGAACACCAA CCTGGACGTC GCTGAGCGAG CCCGGGAGAA CAAGAGCTCC 
CTGAGTGTTG CCCTGGACAA ACTTCGAGCC CAGAAGAGTG GTGACCTGGA GCAACCGGGC 
AGGCTGGTGG TGGAGGTGGC ACTGGGCAAC TTGTCACGGG CTGTGGACCT GCTGCGGAGG 
CACCCAGAGC AGGTGGATAC CAAGAATCAG GGCCGGACTG CCCTGCAGGT GGCTGCCTAC 
CTGGGCCAGG TGAAGCTGGT GCGGCTGCTG CTCCAGGCAA GGGCAGGCAC AGACCTGCCT 
GATGAGGAGG GCAACACTTC ACTGCACTAT GCAGCCCTAG GAAACCAGCC GGAGGTCACC 
CAGGTGCTCC TGAGTGTGGG GTGCCCGGCT GACACCCTCA ACAGCAGCCA GAGCACTGCA 
CTACACCTTG CAGTGCAGAG GGGCTTCCTG GAGGTGGTGC GGGTCCTGTG TGAGCATGGC 
TGTGATGTCA ACCTGCCTGA CTCGAATGCC GACACACCAC TGCACTGCGC CATCTCAGCT 
GGCACGGGGG CCAGTGGCAT AGTGGAGGTC CTCACTGAGG TGCCAGGTGT GGACGTCACC 
ACCACCAACA GCCAGGGCTT CACCCTGCTG CATCACGCCT CCCTCAAGGG CCACACGCTC 
GCTGTCAGGA GGATCCTGGC CCGGGCCCGG CAGCTGGTGG ATGCCAAGAA GGAGGACGGC 
TTCACTGCCT TGCACCTGGC CGCCCTCAAC AATCACCGAG AGGTGGCCCA GATTCTCATC 
CGGGAGGGCC ACTGCGATGT GAATGTGCGT AACCGCAAGC TGCAGACACC CCTGCATCTG 
GCTGTTCAGC AGGCCCACAT GGGGCTGGTG CCCCTGCTGA CCGACGCTGG CTGCAGCATC 
AATGCAGAGG ATGAGGAGGG GGACACAGCC CTGCATGTGG CCCTGCAGCG GCACCAGCTG 
CTGCCCCTGG CAGCTGACCG GGCCGGGGGG GACTCTGGGC CAGCACAGCT ATTGTCCAGG 
CTGCAGGCCT CGGGCCTCCC TGGCTTTGCG GAGTTGACAA TAGGCACTGC CATCGCCTGC 
TTCCTCGCAC TGGAGGGAGC CGATTTGAGC TACGCCAACC ACCGTGGCCG CAGCCCGCTG 
GACCTGGCTG CAGAGGGCCG AGTGCTCAAG GCCCTGCAGG GCTGTGCCCA GCGCTTCCGG 
GAGCGGCAGG TGGGCGCCAC GGCCGCCGGC CCCAGGCAGT TGCGGAGCAC CCCTAACACC 
GTGACCAACC TGCATGTGGC CCCAGCGGCA GGGCATGAGG CGGCCGAGTG CCTCGTGTGC 
TCGGAGCTGG CGCTGCTGGT GCTTTTCACG CCGTGCCAGC ATCGCACTGT GTGCGAGGAG 
TGCGCGCGCA GGATGAAGAA GTGCATCAGG TGCCAGGTGG CCATCAGCAA GAAGCTGCGC 
CCAGACGGCT CGGAGTTGGC CAGCGCAGCC CCTGCGTCTA TGCCGCAGCG GCAGCTTGTA 
GAGGAGCTGC AGAGCCGTTA CCGGCAGATG GAGGAGCGCA TCACCTGCCC AATCTGCATC 
GACAACCACA TCCGCCTGGT TTTCCAGTGC GGCCACGGCG CCTGCGCGCC CTGCGGCGCT 
GCGCTCACTG CCTGCCCCAT CTGCCGCCAG CCGGTCCGCG ACCGAATCCA GATCTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_016191017.1 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_016046503.1 Erinaceus europaeus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X5, mRNA.

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CloneID	OEr17585
Clone ID Related Accession (Same CDS sequence)	XM_016191017.1
Accession Version	XM_016191017.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	E3 ubiquitin-protein ligase MIB2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006804353.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACCCG CACCAACTAC CGTGCGGGCT ATCAGGGTGC CCATGACCTG CTGCTCTACG 
ACAATGCCCA GATCGGTGTG CGGCACCCCA ACATCATCTG CGACTGCTGC AAGAAACACG 
GGTTGCGGGG CATGCGCTGG AAGTGCCTCG TCTGTTTTGA CTATGACCTG TGCACTCAGT 
GTTACATGCA CAACAGACAT GATCTGGCAC ACGCCTTTGA GCGCTACGAG ACTGCACACT 
CGCGCCCGAG GGGAGGGGAA ACCAGGCCGT GTGGTGGACA TCCGGGGCTG GGACGTGGAG 
ACAGGCCGGA GTGTGGCCAG CGTGACCTGG GCAGATGGCA CCACCAACGT GTACCGCGTA 
GGCCACAAGG GCAAGGTGGA CCTCAAGTGT GTGGGCGAGG CAGCCGGCGG CTTCTACTAC 
AAGGAGCACC TCCCCAGGCT GGCCTGCTAT CCCCCAGGCA AGCCGGCTGA ACTGCGACGC 
AGGGTGAGTG TGGATGGGCA GCCCTTCCAG CACGGCGACA AGGTCAAGTG CCTGCTGGAC 
TCGGATGTGC TGAGGGAGAT GCAGGAAGGC CACGGTGGCT GGAATCCCCG GATGGCGGAG 
TGCATTGGAC AGACGGGCAC CGTGCACCGC GTCACAGACC GTGGGGATGT GCGTGTGCAG 
TTCAGCCACG AGACCCGTTG GACCTTCCAC CCAGGGGCTC TCACCAAGCA TGACTGCTTC 
TGGGTGGGTG ATGTGGTGCG GGTCATCGAC GACCTGGACA TGGTGAAGCG GCTGCAGGCT 
GGGCATGGCG AGTGGACAGA CGACATGGCC CCCGCCCTGG GCCGTGTAGG AAAGGTGGTG 
AAGGTCTTTG GCGACGGGAA CCTGCGTGTG GCAGTCAGCG GCCAGCTGTG GACCTTCAGC 
CCCTCATGCC TCGTGGCCTA CCGGCCCGAA GAGAACACCA ACCTGGACGT CGCTGAGCGA 
GCCCGGGAGA ACAAGAGCTC CCTGAGTGTT GCCCTGGACA AACTTCGAGC CCAGAAGAGT 
GGTGACCTGG AGCAACCGGG CAGGCTGGTG GTGGAGGTGG CACTGGGCAA CTTGTCACGG 
GCTGTGGACC TGCTGCGGAG GCACCCAGAG CAGGTGGATA CCAAGAATCA GGGCCGGACT 
GCCCTGCAGG TGGCTGCCTA CCTGGGCCAG GTGAAGCTGG TGCGGCTGCT GCTCCAGGCA 
AGGGCAGGCA CAGACCTGCC TGATGAGGAG GGCAACACTT CACTGCACTA TGCAGCCCTA 
GGAAACCAGC CGGAGGTCAC CCAGGTGCTC CTGAGTGTGG GGTGCCCGGC TGACACCCTC 
AACAGCAGCC AGAGCACTGC ACTACACCTT GCAGTGCAGA GGGGCTTCCT GGAGGTGGTG 
CGGGTCCTGT GTGAGCATGG CTGTGATGTC AACCTGCCTG ACTCGAATGC CGACACACCA 
CTGCACTGCG CCATCTCAGC TGGCACGGGG GCCAGTGGCA TAGTGGAGGT CCTCACTGAG 
GTGCCAGGTG TGGACGTCAC CACCACCAAC AGCCAGGGCT TCACCCTGCT GCATCACGCC 
TCCCTCAAGG GCCACACGCT CGCTGTCAGG AGGATCCTGG CCCGGGCCCG GCAGCTGGTG 
GATGCCAAGA AGGAGGACGG CTTCACTGCC TTGCACCTGG CCGCCCTCAA CAATCACCGA 
GAGGTGGCCC AGATTCTCAT CCGGGAGGGC CACTGCGATG TGAATGTGCG TAACCGCAAG 
CTGCAGACAC CCCTGCATCT GGCTGTTCAG CAGGCCCACA TGGGGCTGGT GCCCCTGCTG 
ACCGACGCTG GCTGCAGCAT CAATGCAGAG GATGAGGAGG GGGACACAGC CCTGCATGTG 
GCCCTGCAGC GGCACCAGCT GCTGCCCCTG GCAGCTGACC GGGCCGGGGG GGACTCTGGG 
CCAGCACAGC TATTGTCCAG GCTGCAGGCC TCGGGCCTCC CTGGCTTTGC GGAGTTGACA 
ATAGGCACTG CCATCGCCTG CTTCCTCGCA CTGGAGGGAG CCGATTTGAG CTACGCCAAC 
CACCGTGGCC GCAGCCCGCT GGACCTGGCT GCAGAGGGCC GAGTGCTCAA GGCCCTGCAG 
GGCTGTGCCC AGCGCTTCCG GGAGCGGCAG GTGGGCGCCA CGGCCGCCGG CCCCAGGCAG 
TTGCGGAGCA CCCCTAACAC CGTGACCAAC CTGCATGTGG CCCCAGCGGC AGGGCATGAG 
GCGGCCGAGT GCCTCGTGTG CTCGGAGCTG GCGCTGCTGG TGCTTTTCAC GCCGTGCCAG 
CATCGCACTG TGTGCGAGGA GTGCGCGCGC AGGATGAAGA AGTGCATCAG GTGCCAGGTG 
GCCATCAGCA AGAAGCTGCG CCCAGACGGC TCGGAGTTGG CCAGCGCAGC CCCTGCGTCT 
ATGCCGCAGC GGCAGCTTGT AGAGGAGCTG CAGAGCCGTT ACCGGCAGAT GGAGGAGCGC 
ATCACCTGCC CAATCTGCAT CGACAACCAC ATCCGCCTGG TTTTCCAGTG CGGCCACGGC 
GCCTGCGCGC CCTGCGGCGC TGCGCTCACT GCCTGCCCCA TCTGCCGCCA GCCGGTCCGC 
GACCGAATCC AGATCTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016046503.1
CDS	3..2606
Translation	MAPAPTTVRAIRVPMTCCSTTMPRSVCGTPTSSATAARNTGCGACAGSASSVLTMTCALSVTCTTDMIWHTPLSATRLHTRARGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKEHLPRLACYPPGKPAELRRRVSVDGQPFQHGDKVKCLLDSDVLREMQEGHGGWNPRMAECIGQTGTVHRVTDRGDVRVQFSHETRWTFHPGALTKHDCFWVGDVVRVIDDLDMVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVSGQLWTFSPSCLVAYRPEENTNLDVAERARENKSSLSVALDKLRAQKSGDLEQPGRLVVEVALGNLSRAVDLLRRHPEQVDTKNQGRTALQVAAYLGQVKLVRLLLQARAGTDLPDEEGNTSLHYAALGNQPEVTQVLLSVGCPADTLNSSQSTALHLAVQRGFLEVVRVLCEHGCDVNLPDSNADTPLHCAISAGTGASGIVEVLTEVPGVDVTTTNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGHCDVNVRNRKLQTPLHLAVQQAHMGLVPLLTDAGCSINAEDEEGDTALHVALQRHQLLPLAADRAGGDSGPAQLLSRLQASGLPGFAELTIGTAIACFLALEGADLSYANHRGRSPLDLAAEGRVLKALQGCAQRFRERQVGATAAGPRQLRSTPNTVTNLHVAPAAGHEAAECLVCSELALLVLFTPCQHRTVCEECARRMKKCIRCQVAISKKLRPDGSELASAAPASMPQRQLVEELQSRYRQMEERITCPICIDNHIRLVFQCGHGACAPCGAALTACPICRQPVRDRIQIFV

Target ORF information:

RefSeq Version	XM_016191017.1
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016191017.1

ORF Insert Sequence:

ATGGCACCCG CACCAACTAC CGTGCGGGCT ATCAGGGTGC CCATGACCTG CTGCTCTACG 
ACAATGCCCA GATCGGTGTG CGGCACCCCA ACATCATCTG CGACTGCTGC AAGAAACACG 
GGTTGCGGGG CATGCGCTGG AAGTGCCTCG TCTGTTTTGA CTATGACCTG TGCACTCAGT 
GTTACATGCA CAACAGACAT GATCTGGCAC ACGCCTTTGA GCGCTACGAG ACTGCACACT 
CGCGCCCGAG GGGAGGGGAA ACCAGGCCGT GTGGTGGACA TCCGGGGCTG GGACGTGGAG 
ACAGGCCGGA GTGTGGCCAG CGTGACCTGG GCAGATGGCA CCACCAACGT GTACCGCGTA 
GGCCACAAGG GCAAGGTGGA CCTCAAGTGT GTGGGCGAGG CAGCCGGCGG CTTCTACTAC 
AAGGAGCACC TCCCCAGGCT GGCCTGCTAT CCCCCAGGCA AGCCGGCTGA ACTGCGACGC 
AGGGTGAGTG TGGATGGGCA GCCCTTCCAG CACGGCGACA AGGTCAAGTG CCTGCTGGAC 
TCGGATGTGC TGAGGGAGAT GCAGGAAGGC CACGGTGGCT GGAATCCCCG GATGGCGGAG 
TGCATTGGAC AGACGGGCAC CGTGCACCGC GTCACAGACC GTGGGGATGT GCGTGTGCAG 
TTCAGCCACG AGACCCGTTG GACCTTCCAC CCAGGGGCTC TCACCAAGCA TGACTGCTTC 
TGGGTGGGTG ATGTGGTGCG GGTCATCGAC GACCTGGACA TGGTGAAGCG GCTGCAGGCT 
GGGCATGGCG AGTGGACAGA CGACATGGCC CCCGCCCTGG GCCGTGTAGG AAAGGTGGTG 
AAGGTCTTTG GCGACGGGAA CCTGCGTGTG GCAGTCAGCG GCCAGCTGTG GACCTTCAGC 
CCCTCATGCC TCGTGGCCTA CCGGCCCGAA GAGAACACCA ACCTGGACGT CGCTGAGCGA 
GCCCGGGAGA ACAAGAGCTC CCTGAGTGTT GCCCTGGACA AACTTCGAGC CCAGAAGAGT 
GGTGACCTGG AGCAACCGGG CAGGCTGGTG GTGGAGGTGG CACTGGGCAA CTTGTCACGG 
GCTGTGGACC TGCTGCGGAG GCACCCAGAG CAGGTGGATA CCAAGAATCA GGGCCGGACT 
GCCCTGCAGG TGGCTGCCTA CCTGGGCCAG GTGAAGCTGG TGCGGCTGCT GCTCCAGGCA 
AGGGCAGGCA CAGACCTGCC TGATGAGGAG GGCAACACTT CACTGCACTA TGCAGCCCTA 
GGAAACCAGC CGGAGGTCAC CCAGGTGCTC CTGAGTGTGG GGTGCCCGGC TGACACCCTC 
AACAGCAGCC AGAGCACTGC ACTACACCTT GCAGTGCAGA GGGGCTTCCT GGAGGTGGTG 
CGGGTCCTGT GTGAGCATGG CTGTGATGTC AACCTGCCTG ACTCGAATGC CGACACACCA 
CTGCACTGCG CCATCTCAGC TGGCACGGGG GCCAGTGGCA TAGTGGAGGT CCTCACTGAG 
GTGCCAGGTG TGGACGTCAC CACCACCAAC AGCCAGGGCT TCACCCTGCT GCATCACGCC 
TCCCTCAAGG GCCACACGCT CGCTGTCAGG AGGATCCTGG CCCGGGCCCG GCAGCTGGTG 
GATGCCAAGA AGGAGGACGG CTTCACTGCC TTGCACCTGG CCGCCCTCAA CAATCACCGA 
GAGGTGGCCC AGATTCTCAT CCGGGAGGGC CACTGCGATG TGAATGTGCG TAACCGCAAG 
CTGCAGACAC CCCTGCATCT GGCTGTTCAG CAGGCCCACA TGGGGCTGGT GCCCCTGCTG 
ACCGACGCTG GCTGCAGCAT CAATGCAGAG GATGAGGAGG GGGACACAGC CCTGCATGTG 
GCCCTGCAGC GGCACCAGCT GCTGCCCCTG GCAGCTGACC GGGCCGGGGG GGACTCTGGG 
CCAGCACAGC TATTGTCCAG GCTGCAGGCC TCGGGCCTCC CTGGCTTTGC GGAGTTGACA 
ATAGGCACTG CCATCGCCTG CTTCCTCGCA CTGGAGGGAG CCGATTTGAG CTACGCCAAC 
CACCGTGGCC GCAGCCCGCT GGACCTGGCT GCAGAGGGCC GAGTGCTCAA GGCCCTGCAG 
GGCTGTGCCC AGCGCTTCCG GGAGCGGCAG GTGGGCGCCA CGGCCGCCGG CCCCAGGCAG 
TTGCGGAGCA CCCCTAACAC CGTGACCAAC CTGCATGTGG CCCCAGCGGC AGGGCATGAG 
GCGGCCGAGT GCCTCGTGTG CTCGGAGCTG GCGCTGCTGG TGCTTTTCAC GCCGTGCCAG 
CATCGCACTG TGTGCGAGGA GTGCGCGCGC AGGATGAAGA AGTGCATCAG GTGCCAGGTG 
GCCATCAGCA AGAAGCTGCG CCCAGACGGC TCGGAGTTGG CCAGCGCAGC CCCTGCGTCT 
ATGCCGCAGC GGCAGCTTGT AGAGGAGCTG CAGAGCCGTT ACCGGCAGAT GGAGGAGCGC 
ATCACCTGCC CAATCTGCAT CGACAACCAC ATCCGCCTGG TTTTCCAGTG CGGCCACGGC 
GCCTGCGCGC CCTGCGGCGC TGCGCTCACT GCCTGCCCCA TCTGCCGCCA GCCGGTCCGC 
GACCGAATCC AGATCTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MIB2 cDNA ORF clone, Erinaceus europaeus(western European hedgehog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム