NEFM cDNA ORF clone, Erinaceus europaeus(western European hedgehog)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEFM
Entrez Gene ID	103118681
Full Name	neurofilament medium
Gene Type	protein-coding
Organism	Erinaceus europaeus(western European hedgehog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007528886.2	XP_007528948.1	neurofilament medium polypeptide

Pathways from BioSystems
Gene Ontology

The following NEFM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEFM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEr16960	XM_007528886.2 Latest version!	Erinaceus europaeus neurofilament, medium polypeptide (NEFM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEFM ( XM_007528886.2 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_007528948.1 Erinaceus europaeus neurofilament, medium polypeptide (NEFM), mRNA.

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CloneID	OEr16960
Clone ID Related Accession (Same CDS sequence)	XM_007528886.2
Accession Version	XM_007528886.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	neurofilament medium polypeptide
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006804326.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 11, 2016 this sequence version replaced XM_007528886.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACA CGCTTGACTC GCTGGGCAAC CCGTCCGCCT ACCGGCGGGT GACCGAAACC 
CGCTCGAGCT TCAGCCGGGT GAGCGGCTCG CCGTCCAGCG GCTTCCGCTC GCAGTCGTGG 
TCGCGGAGCT CACCCAGCAC CGTGTCCTCG TCCTACAAGC GCAGCGCGCT GGCCCCGCGG 
CTCGCCTACA GCGCGGCCGT GCTCAGCTCG GCCGAGAGCA GCCTCGACTT CAGCCAGTCC 
TCGTCCCTGC TCAACGGCGG CGCCGGGCCC GGCGGCGACT ACAAGCTGGC CCGCTCCAAC 
GAGAAGGAGC AGCTGCAGGG GCTCAACGAC CGCTTCGCCG GCTACATCGA GAAGGTGCAC 
TACCTGGAGC AGCAGAACAA GGAGATCGAG GCCGAGATCC AGGCGCTGCG GCAGAAGCAG 
GCTACGCAGG CCCAGCTGAG CGATGCGTAC GACCAGGAGA TCCGCGAGCT GCGCACCACG 
CTGGAGATGG TGAACCACGA GAAGGCGCAG GTCCAGCTCG ACTCGGACCA CCTGGAGGAA 
GACATCCACC GGCTCAAGGA GCGCTTCGAG GAGGAGGCGC GGCTACGCGA CGACACCGAG 
GCGGCCATCC GGGCACTGCG CAAAGACATC GAGGAGTCAT CGATGGTCAA GGTGGAGCTG 
GACAAGAAGG TGCAGTCGCT GCAGGATGAG GTGGCCTTCC TGCGGAGCAA CCACGAAGAG 
GAGGTGGCCG ACCTGCTGGC CCAGATCCAG GCGTCGCACA TCACGGTGGA GCGCAAAGAC 
TACCTGAAGA CGGACATCTC GTCGGCGCTG AAGGAGATCC GCTCCCAGCT CGAGTGCCAC 
TCGGATCAGA ACATGCACCA GGCCGAGGAG TGGTTCAAGT GCCGCTACGC CAAGCTCACC 
GAGGCGGCCG AGCAGAACAA GGAAGCCATA CGCTCCGCCA AGGAAGAGAT CGCCGAGTAC 
CGGCGCCAGC TGCAGTCCAA GAGTATCGAG CTCGAGTCGG TGCGCGGCAC CAAGGAGTCC 
CTGGAGCGGC AGCTCAACGA CATCGAGGAG CGCCACAACC ACGACCTTAG CAGCTACCAG 
GATACCATCC AGCAATTGGA AAATGAGCTT CGGGGCACTA AGTGGGAAAT GGCTCGTCAT 
CTGCGAGAAT ACCAGGATCT CCTCAATGTC AAGATGGCTC TGGATATCGA AATTGCCGCC 
TACAGAAAAC TCCTGGAGGG TGAAGAGACC AGATTTAGCA CATTTTCAGG AAGCATCACT 
GGGCCCATAT ATACACACCG ACAGCCCTCA ATCACAATAT CCAGCAAAAT CCAAAAAACC 
AAAGTAGAGG CTCCCAAGCT AAAGGTTCAA CACAAATTTG TTGAGGAAAT CATAGAAGAA 
ACCAAAGTCG AAGATGAGAA GTCAGAAATG GAAGATGCCT TGACAGCCAT TGCAGAGGAA 
CTGGGTGTTT CCATGAAGGA AGAAGCAAAA GAAGAGACAG AAGAAAAGGA AGAGGAACAA 
GACACTGAGG AAGAAGTGGT AGCTACCAAA AAATCTCCAG TGAAAGCTAT GGCACCAGAA 
TTTAAAGAAG GGGAAAAGGA GGAGGAAGAA GAAGGCCAAG AGGAAGAAGA GGAGGAGGAA 
GATGAAGGTG CAAAATCAGA CCAGGCTGAA GAAGGAGGGT CTGAGAAGGA AGGTTCTAGT 
GAAAAAGAAG AAGGTGAGCA GGAAGAAGAA GAAGTGGAGG CTGAAGGAGG AGAGGAAACT 
GCAGCAAAGG ATGAAAAGAA ACCAGAGGAG AAGACTGAAG TCGTGGCTGC TAAAGAGTTA 
GTGGTAGAAA AATCAGAGAA AGCCAAATCA CCAATGCCCA AATCACCCAA AGAGGAAGAA 
GGAAAGAAAG AAGTAAAGGA AACTCCCAAA GAAGAGAAGG CAGAGAAAAA GGAGGAGAAG 
CCAAAAGATG TGCCAGAGAA GAAAGCTGAG TCTCCAGCAA AGGAGAAAGC TGAGTCTCCG 
GTAAAGGAAA AGTCAGAATC CCCAGTGAAG GAGAAAGCAG AATCTCCAGT GAAGGAGAAA 
GCAGAATCTC CAGTGAAGGA GAAAGCAGAA TCTCCAGTGA AGGAGAAAGC AGAATCTCCA 
GCAAAAGAGA AAGCTGAGTC TCCAGTAAAG GAAAAGGCTG AATCCCCAGT AAAGGAAAAG 
GCTCAATCCC CAGTGAAGGA AAAAGCTGAA TCCCCAGTAA AGGAAAAGGA AAAGGCTGAA 
TCCCCAGTAA AGGAGAAGGT TGTGGAGGAG GTGGTCACCA TAACCAAATC AGTAAAGGTG 
AGCTTAGAGA AAGAGGCCAA AGAGGAGAAG CCGCAGAAGC AGAAGGAGAA AGCAGATGAG 
AAAGGAGGAA GTGAGGAGGA AGGCAGTGAT CAGGGTTCCA AGGGCTCAGC AAAGGAAGAC 
ATAGCTGTAA ATGGGGAGGT AGAAGGCAAG GAGGGGGAAG AACAGGAAAC AAAGGAGAAA 
GGTAGTGGAG GTGAAGAGGA GAAAGGGGTC ATCACCAATG GGCTAGACTT GAGCCCAGCT 
GATGAAAAGA AGGGGGATGA CAGGAGTGAG GAAAAAGTGG TGGTAACCAA GACAGTAGAA 
AAAATCACCA GTGAGGGGGG AGATGGTGCT ACCAAATACA TTACCAAATC TGTAACCGTC 
ACTCAAAAGG TCGAAGAGCA TGAAGAGACC TTTGAGGAGA AACTAGTGTC TACTAAAAAG 
GTAGAGAAGG TTACTTCACA TGCCATAGTA AAGGAAGTCA CCCAGAGTGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007528948.1
CDS	21..2774
Translation	MSYTLDSLGNPSAYRRVTETRSSFSRVSGSPSSGFRSQSWSRSSPSTVSSSYKRSALAPRLAYSAAVLSSAESSLDFSQSSSLLNGGAGPGGDYKLARSNEKEQLQGLNDRFAGYIEKVHYLEQQNKEIEAEIQALRQKQATQAQLSDAYDQEIRELRTTLEMVNHEKAQVQLDSDHLEEDIHRLKERFEEEARLRDDTEAAIRALRKDIEESSMVKVELDKKVQSLQDEVAFLRSNHEEEVADLLAQIQASHITVERKDYLKTDISSALKEIRSQLECHSDQNMHQAEEWFKCRYAKLTEAAEQNKEAIRSAKEEIAEYRRQLQSKSIELESVRGTKESLERQLNDIEERHNHDLSSYQDTIQQLENELRGTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFSGSITGPIYTHRQPSITISSKIQKTKVEAPKLKVQHKFVEEIIEETKVEDEKSEMEDALTAIAEELGVSMKEEAKEETEEKEEEQDTEEEVVATKKSPVKAMAPEFKEGEKEEEEEGQEEEEEEEDEGAKSDQAEEGGSEKEGSSEKEEGEQEEEEVEAEGGEETAAKDEKKPEEKTEVVAAKELVVEKSEKAKSPMPKSPKEEEGKKEVKETPKEEKAEKKEEKPKDVPEKKAESPAKEKAESPVKEKSESPVKEKAESPVKEKAESPVKEKAESPVKEKAESPAKEKAESPVKEKAESPVKEKAQSPVKEKAESPVKEKEKAESPVKEKVVEEVVTITKSVKVSLEKEAKEEKPQKQKEKADEKGGSEEEGSDQGSKGSAKEDIAVNGEVEGKEGEEQETKEKGSGGEEEKGVITNGLDLSPADEKKGDDRSEEKVVVTKTVEKITSEGGDGATKYITKSVTVTQKVEEHEETFEEKLVSTKKVEKVTSHAIVKEVTQSD

Target ORF information:

RefSeq Version	XM_007528886.2
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus neurofilament, medium polypeptide (NEFM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007528886.2

ORF Insert Sequence:

ATGAGCTACA CGCTTGACTC GCTGGGCAAC CCGTCCGCCT ACCGGCGGGT GACCGAAACC 
CGCTCGAGCT TCAGCCGGGT GAGCGGCTCG CCGTCCAGCG GCTTCCGCTC GCAGTCGTGG 
TCGCGGAGCT CACCCAGCAC CGTGTCCTCG TCCTACAAGC GCAGCGCGCT GGCCCCGCGG 
CTCGCCTACA GCGCGGCCGT GCTCAGCTCG GCCGAGAGCA GCCTCGACTT CAGCCAGTCC 
TCGTCCCTGC TCAACGGCGG CGCCGGGCCC GGCGGCGACT ACAAGCTGGC CCGCTCCAAC 
GAGAAGGAGC AGCTGCAGGG GCTCAACGAC CGCTTCGCCG GCTACATCGA GAAGGTGCAC 
TACCTGGAGC AGCAGAACAA GGAGATCGAG GCCGAGATCC AGGCGCTGCG GCAGAAGCAG 
GCTACGCAGG CCCAGCTGAG CGATGCGTAC GACCAGGAGA TCCGCGAGCT GCGCACCACG 
CTGGAGATGG TGAACCACGA GAAGGCGCAG GTCCAGCTCG ACTCGGACCA CCTGGAGGAA 
GACATCCACC GGCTCAAGGA GCGCTTCGAG GAGGAGGCGC GGCTACGCGA CGACACCGAG 
GCGGCCATCC GGGCACTGCG CAAAGACATC GAGGAGTCAT CGATGGTCAA GGTGGAGCTG 
GACAAGAAGG TGCAGTCGCT GCAGGATGAG GTGGCCTTCC TGCGGAGCAA CCACGAAGAG 
GAGGTGGCCG ACCTGCTGGC CCAGATCCAG GCGTCGCACA TCACGGTGGA GCGCAAAGAC 
TACCTGAAGA CGGACATCTC GTCGGCGCTG AAGGAGATCC GCTCCCAGCT CGAGTGCCAC 
TCGGATCAGA ACATGCACCA GGCCGAGGAG TGGTTCAAGT GCCGCTACGC CAAGCTCACC 
GAGGCGGCCG AGCAGAACAA GGAAGCCATA CGCTCCGCCA AGGAAGAGAT CGCCGAGTAC 
CGGCGCCAGC TGCAGTCCAA GAGTATCGAG CTCGAGTCGG TGCGCGGCAC CAAGGAGTCC 
CTGGAGCGGC AGCTCAACGA CATCGAGGAG CGCCACAACC ACGACCTTAG CAGCTACCAG 
GATACCATCC AGCAATTGGA AAATGAGCTT CGGGGCACTA AGTGGGAAAT GGCTCGTCAT 
CTGCGAGAAT ACCAGGATCT CCTCAATGTC AAGATGGCTC TGGATATCGA AATTGCCGCC 
TACAGAAAAC TCCTGGAGGG TGAAGAGACC AGATTTAGCA CATTTTCAGG AAGCATCACT 
GGGCCCATAT ATACACACCG ACAGCCCTCA ATCACAATAT CCAGCAAAAT CCAAAAAACC 
AAAGTAGAGG CTCCCAAGCT AAAGGTTCAA CACAAATTTG TTGAGGAAAT CATAGAAGAA 
ACCAAAGTCG AAGATGAGAA GTCAGAAATG GAAGATGCCT TGACAGCCAT TGCAGAGGAA 
CTGGGTGTTT CCATGAAGGA AGAAGCAAAA GAAGAGACAG AAGAAAAGGA AGAGGAACAA 
GACACTGAGG AAGAAGTGGT AGCTACCAAA AAATCTCCAG TGAAAGCTAT GGCACCAGAA 
TTTAAAGAAG GGGAAAAGGA GGAGGAAGAA GAAGGCCAAG AGGAAGAAGA GGAGGAGGAA 
GATGAAGGTG CAAAATCAGA CCAGGCTGAA GAAGGAGGGT CTGAGAAGGA AGGTTCTAGT 
GAAAAAGAAG AAGGTGAGCA GGAAGAAGAA GAAGTGGAGG CTGAAGGAGG AGAGGAAACT 
GCAGCAAAGG ATGAAAAGAA ACCAGAGGAG AAGACTGAAG TCGTGGCTGC TAAAGAGTTA 
GTGGTAGAAA AATCAGAGAA AGCCAAATCA CCAATGCCCA AATCACCCAA AGAGGAAGAA 
GGAAAGAAAG AAGTAAAGGA AACTCCCAAA GAAGAGAAGG CAGAGAAAAA GGAGGAGAAG 
CCAAAAGATG TGCCAGAGAA GAAAGCTGAG TCTCCAGCAA AGGAGAAAGC TGAGTCTCCG 
GTAAAGGAAA AGTCAGAATC CCCAGTGAAG GAGAAAGCAG AATCTCCAGT GAAGGAGAAA 
GCAGAATCTC CAGTGAAGGA GAAAGCAGAA TCTCCAGTGA AGGAGAAAGC AGAATCTCCA 
GCAAAAGAGA AAGCTGAGTC TCCAGTAAAG GAAAAGGCTG AATCCCCAGT AAAGGAAAAG 
GCTCAATCCC CAGTGAAGGA AAAAGCTGAA TCCCCAGTAA AGGAAAAGGA AAAGGCTGAA 
TCCCCAGTAA AGGAGAAGGT TGTGGAGGAG GTGGTCACCA TAACCAAATC AGTAAAGGTG 
AGCTTAGAGA AAGAGGCCAA AGAGGAGAAG CCGCAGAAGC AGAAGGAGAA AGCAGATGAG 
AAAGGAGGAA GTGAGGAGGA AGGCAGTGAT CAGGGTTCCA AGGGCTCAGC AAAGGAAGAC 
ATAGCTGTAA ATGGGGAGGT AGAAGGCAAG GAGGGGGAAG AACAGGAAAC AAAGGAGAAA 
GGTAGTGGAG GTGAAGAGGA GAAAGGGGTC ATCACCAATG GGCTAGACTT GAGCCCAGCT 
GATGAAAAGA AGGGGGATGA CAGGAGTGAG GAAAAAGTGG TGGTAACCAA GACAGTAGAA 
AAAATCACCA GTGAGGGGGG AGATGGTGCT ACCAAATACA TTACCAAATC TGTAACCGTC 
ACTCAAAAGG TCGAAGAGCA TGAAGAGACC TTTGAGGAGA AACTAGTGTC TACTAAAAAG 
GTAGAGAAGG TTACTTCACA TGCCATAGTA AAGGAAGTCA CCCAGAGTGA CTAA