LOC111159777 cDNA ORF clone, Enhydra lutris kenyoni

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC111159777
Entrez Gene ID	111159777
Full Name	sodium channel epithelial 1 alpha subunit
Gene Type	protein-coding
Organism	Enhydra lutris kenyoni

mRNA and Protein(s)

mRNA	Protein	Name
XM_022522378.1	XP_022378086.1	amiloride-sensitive sodium channel subunit alpha

Pathways from BioSystems
Gene Ontology

The following LOC111159777 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC111159777 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEn50204	XM_022522378.1 Latest version!	Enhydra lutris kenyoni sodium channel epithelial 1 alpha subunit (LOC111159777), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC111159777 ( XM_022522378.1 ) cDNA ORF clone, Enhydra lutris kenyoni -> XP_022378086.1 Enhydra lutris kenyoni sodium channel epithelial 1 alpha subunit (LOC111159777), mRNA.

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CloneID	OEn50204
Clone ID Related Accession (Same CDS sequence)	XM_022522378.1
Accession Version	XM_022522378.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-19
Organism	Enhydra lutris kenyoni
Product	amiloride-sensitive sodium channel subunit alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019154153.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Enhydra lutris kenyoni Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGGAG AGCAGCGTGA GGAGCAGGGG CCCGGCCCCG AACCCGCGGC CCCCCCGCAG 
CCCACAGAAG AGGAGGAGGC CCTGATCGAG TTCCACCGCT CCTACCGAGA GCTCTTCCAG 
TTCTTCTGCA ACAACACCAC CATCCACGGC GCCATCCGAC TGGTGTGCTC CCAGCACAAC 
CGCATGAAGA CGGCCTTCTG GGCCGTGCTG TGGCTCTGCG CCTTCTGCAT GATGTACTGG 
CAGTTCGGCC AGCTGTTCGG GGAGTACTTC AGCTACCCCG TCAGCCTCAA CATCAACCTC 
AACTCCGACA AGCTCGTCTT CCCCGCGGTC ACCATCTGCA ACCTCAACCC CTACAGGGAC 
ACGCAAATTA AAGGGGAGCT GGAGGAACTG GACCGCATCA CCGAGCAGAC GCTCTACGAT 
CTGTACAAGT ACAACTCTTC CAGCACCGTG GGGGCCCACC CCCGCGGTCG TCGCGACCTC 
GGGGAGACTT GGCCGCACCC CCTACAGCGC CTGGCGGTCC CGGCCCCGCC CTCCCGGGCC 
CGCCAAGCCC GCAGCGCCAA ATCGATTGTG CAGGACAACA ACCCCCAAGT GGACAGGAAG 
GACTGGAAGA TCGGCTTCCA GCTGTGCAAC CAGAACAAAT CGGACTGCTT TTACCAGACT 
TACTCGTCAG GGGTGGATGC AGTGAGGGAG TGGTACCGTT TCCACTACAT CAACATTCTT 
GCGAGACTGC GAGACACTTC TCCGGCCCCG GGGGAGGACA TGCTGGACAA CTTCATCTTC 
GCCTGCCGCT TCAACCAGGC CTCCTGCAGT CAGGCGAATT ACTCTCACTT TCATCACCCG 
ATGTATGGGA ACTGCTACAC ATTCAATGGC AAGAACAGCT CTAACCTCTG GATGTCTTCT 
ATGCCTGGGA TCAAAAATGG TCTGTCCCTA ACACTGCGCA CAGAGCAGAA TGACTTCATC 
CCGCTGCTGT CCACAGTGAC TGGGGCCAGG GTCATGGTCC ATGGGCAGGA TGAGCCTGCC 
TTCATGGATG ACGGTGGCTT TAACTTGCGG CCTGGTGTGG AGACCTCCAT CAGCATGAGG 
AAGGAAGCAC TGGACAGGCT TGGGGGCAAC TATGGTGACT GTACCAAGAA TGGCAGTGAT 
GTCCCTGTGG AGAACCTTTA TCTTTCCAAG TACACACAGC AGGTGTGCAT CCGCTCCTGC 
TTCCAGGAGC ACATGATCAA GCAGTGTGGC TGTGCCTACA TCTTCTATCC ACTGCCCAAG 
AATGCGGAGT TTTGTGACTA CAGGAAGCAC AATTCTTGGG GTTATTGTTA CTATAAGCTC 
CAGGATGCCT TTTCCTCGGA CCGCCTGGGC TGTTTCACCA AGTGCCGGAA GCCATGCAGT 
GTGACCGGCT ACCAGCTATC TGCTGGTTAT TCACGATGGC CCTCAGTGAC ATCTCAGGAC 
TGGGTCTTCC GGATGCTATC CCAACAGAAC AATTATACTA TTAACAACAA AAGAAATGGC 
GTTGCCAAAC TCAACATCTT CTTCAAGGAG CTGAAATACA AAACCAACTC TGAGTCTCCC 
TCTGTCACGA TGGTCACCCT CCTGTCCAAC CTGGGGAGCC AGTGGAGCCT GTGGTTCGGC 
TCCTCGGTGC TGTCTGTGGT GGAGATGGCT GAGCTCGTCT TTGACCTCCT GGTCATTACA 
GTCCTCATGC TACTCCGGAG GCTCCGAAGC CGCTACTGGT CTCCAGGCCG AGGGGGCAGG 
GGTGCCCGGG AGGTGGCCTC CACTCCAGCC TCCTCCGTCC CCTCCCATTT CTGCCCCCAC 
GCCACATCCC CCTCCTCATC CCCACCAGGC CCTGCGACTT CCCCAGCCTT GACAGCCCCT 
CCCCCTGCCT ATGCCTCCTT GGACCCCTGC CCATCTCCAT CCAGCTTGGT GAGGACCAGC 
GCCTCTGTCT GTACTCTGGG GGAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022378086.1
CDS	292..2241
Translation	MKGEQREEQGPGPEPAAPPQPTEEEEALIEFHRSYRELFQFFCNNTTIHGAIRLVCSQHNRMKTAFWAVLWLCAFCMMYWQFGQLFGEYFSYPVSLNINLNSDKLVFPAVTICNLNPYRDTQIKGELEELDRITEQTLYDLYKYNSSSTVGAHPRGRRDLGETWPHPLQRLAVPAPPSRARQARSAKSIVQDNNPQVDRKDWKIGFQLCNQNKSDCFYQTYSSGVDAVREWYRFHYINILARLRDTSPAPGEDMLDNFIFACRFNQASCSQANYSHFHHPMYGNCYTFNGKNSSNLWMSSMPGIKNGLSLTLRTEQNDFIPLLSTVTGARVMVHGQDEPAFMDDGGFNLRPGVETSISMRKEALDRLGGNYGDCTKNGSDVPVENLYLSKYTQQVCIRSCFQEHMIKQCGCAYIFYPLPKNAEFCDYRKHNSWGYCYYKLQDAFSSDRLGCFTKCRKPCSVTGYQLSAGYSRWPSVTSQDWVFRMLSQQNNYTINNKRNGVAKLNIFFKELKYKTNSESPSVTMVTLLSNLGSQWSLWFGSSVLSVVEMAELVFDLLVITVLMLLRRLRSRYWSPGRGGRGAREVASTPASSVPSHFCPHATSPSSSPPGPATSPALTAPPPAYASLDPCPSPSSLVRTSASVCTLGEP

Target ORF information:

RefSeq Version	XM_022522378.1
Organism	Enhydra lutris kenyoni
Definition	Enhydra lutris kenyoni sodium channel epithelial 1 alpha subunit (LOC111159777), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022522378.1

ORF Insert Sequence:

ATGAAAGGAG AGCAGCGTGA GGAGCAGGGG CCCGGCCCCG AACCCGCGGC CCCCCCGCAG 
CCCACAGAAG AGGAGGAGGC CCTGATCGAG TTCCACCGCT CCTACCGAGA GCTCTTCCAG 
TTCTTCTGCA ACAACACCAC CATCCACGGC GCCATCCGAC TGGTGTGCTC CCAGCACAAC 
CGCATGAAGA CGGCCTTCTG GGCCGTGCTG TGGCTCTGCG CCTTCTGCAT GATGTACTGG 
CAGTTCGGCC AGCTGTTCGG GGAGTACTTC AGCTACCCCG TCAGCCTCAA CATCAACCTC 
AACTCCGACA AGCTCGTCTT CCCCGCGGTC ACCATCTGCA ACCTCAACCC CTACAGGGAC 
ACGCAAATTA AAGGGGAGCT GGAGGAACTG GACCGCATCA CCGAGCAGAC GCTCTACGAT 
CTGTACAAGT ACAACTCTTC CAGCACCGTG GGGGCCCACC CCCGCGGTCG TCGCGACCTC 
GGGGAGACTT GGCCGCACCC CCTACAGCGC CTGGCGGTCC CGGCCCCGCC CTCCCGGGCC 
CGCCAAGCCC GCAGCGCCAA ATCGATTGTG CAGGACAACA ACCCCCAAGT GGACAGGAAG 
GACTGGAAGA TCGGCTTCCA GCTGTGCAAC CAGAACAAAT CGGACTGCTT TTACCAGACT 
TACTCGTCAG GGGTGGATGC AGTGAGGGAG TGGTACCGTT TCCACTACAT CAACATTCTT 
GCGAGACTGC GAGACACTTC TCCGGCCCCG GGGGAGGACA TGCTGGACAA CTTCATCTTC 
GCCTGCCGCT TCAACCAGGC CTCCTGCAGT CAGGCGAATT ACTCTCACTT TCATCACCCG 
ATGTATGGGA ACTGCTACAC ATTCAATGGC AAGAACAGCT CTAACCTCTG GATGTCTTCT 
ATGCCTGGGA TCAAAAATGG TCTGTCCCTA ACACTGCGCA CAGAGCAGAA TGACTTCATC 
CCGCTGCTGT CCACAGTGAC TGGGGCCAGG GTCATGGTCC ATGGGCAGGA TGAGCCTGCC 
TTCATGGATG ACGGTGGCTT TAACTTGCGG CCTGGTGTGG AGACCTCCAT CAGCATGAGG 
AAGGAAGCAC TGGACAGGCT TGGGGGCAAC TATGGTGACT GTACCAAGAA TGGCAGTGAT 
GTCCCTGTGG AGAACCTTTA TCTTTCCAAG TACACACAGC AGGTGTGCAT CCGCTCCTGC 
TTCCAGGAGC ACATGATCAA GCAGTGTGGC TGTGCCTACA TCTTCTATCC ACTGCCCAAG 
AATGCGGAGT TTTGTGACTA CAGGAAGCAC AATTCTTGGG GTTATTGTTA CTATAAGCTC 
CAGGATGCCT TTTCCTCGGA CCGCCTGGGC TGTTTCACCA AGTGCCGGAA GCCATGCAGT 
GTGACCGGCT ACCAGCTATC TGCTGGTTAT TCACGATGGC CCTCAGTGAC ATCTCAGGAC 
TGGGTCTTCC GGATGCTATC CCAACAGAAC AATTATACTA TTAACAACAA AAGAAATGGC 
GTTGCCAAAC TCAACATCTT CTTCAAGGAG CTGAAATACA AAACCAACTC TGAGTCTCCC 
TCTGTCACGA TGGTCACCCT CCTGTCCAAC CTGGGGAGCC AGTGGAGCCT GTGGTTCGGC 
TCCTCGGTGC TGTCTGTGGT GGAGATGGCT GAGCTCGTCT TTGACCTCCT GGTCATTACA 
GTCCTCATGC TACTCCGGAG GCTCCGAAGC CGCTACTGGT CTCCAGGCCG AGGGGGCAGG 
GGTGCCCGGG AGGTGGCCTC CACTCCAGCC TCCTCCGTCC CCTCCCATTT CTGCCCCCAC 
GCCACATCCC CCTCCTCATC CCCACCAGGC CCTGCGACTT CCCCAGCCTT GACAGCCCCT 
CCCCCTGCCT ATGCCTCCTT GGACCCCTGC CCATCTCCAT CCAGCTTGGT GAGGACCAGC 
GCCTCTGTCT GTACTCTGGG GGAGCCCTGA