LOC111139221 cDNA ORF clone, Enhydra lutris kenyoni

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC111139221
Entrez Gene ID	111139221
Full Name	LLGL1, scribble cell polarity complex component
Gene Type	protein-coding
Organism	Enhydra lutris kenyoni

mRNA and Protein(s)

mRNA	Protein	Name
XM_022491422.1	XP_022347130.1	lethal(2) giant larvae protein homolog 1 isoform X1
XM_022491423.1	XP_022347131.1	lethal(2) giant larvae protein homolog 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC111139221 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC111139221 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEn54773	XM_022491422.1 Latest version!	Enhydra lutris kenyoni LLGL1, scribble cell polarity complex component (LOC111139221), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OEn54774	XM_022491423.1 Latest version!	Enhydra lutris kenyoni LLGL1, scribble cell polarity complex component (LOC111139221), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC111139221 ( XM_022491422.1 ) cDNA ORF clone, Enhydra lutris kenyoni -> XP_022347130.1 Enhydra lutris kenyoni LLGL1, scribble cell polarity complex component (LOC111139221), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OEn54773
Clone ID Related Accession (Same CDS sequence)	XM_022491422.1
Accession Version	XM_022491422.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-19
Organism	Enhydra lutris kenyoni
Product	lethal(2) giant larvae protein homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019154181.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Enhydra lutris kenyoni Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGGC GCCCATCCTC ACCGTGCAGC TACACTAGTC TGGAGGCTGC CTTGGGGTGG 
GCCTCAGGTG GGCCAGGGGC TGCGTGCCGC TGCCCATGCC CTCCCCTCCC CCTGCAGACC 
GTGGAGCATG GCTTCCCCAA CCAGCCCAGC GCCCTGGCCT TTGACCCAGA GCTCCGCATC 
ATGGCCATCG GCACGAGGTC TGGGGCCGTC AAGATCTATG GTGCACCTGG TGTGGAGTTT 
ACCGGCCTGC ACCAGGACAC AGCCACTGTC ACCCAGATGC ACTTCCTGCC TGGCCAGGGC 
CGCCTCCTGA CCCTGTTGGA CGATAGCAGC CTGCACCTCT GGGAGATTGC CCACCGCAAC 
GGCTGCGCTC ACCTGGAGGA GGCTCTCAGC TTCCAGCCAC CGAGTCGGCC GGGCTTTGAT 
GGGGCCAGTG GCCCGCTCAG CCTTGCCCGC GTCACAGTGG TCCTGCTGGT GGCGGCTGGT 
GACATGGCAG CCCTGGGCAC TGAGGGCGGC AGCATCTTCT TCCTGGATGT TCCCACCCTG 
ACACTGCTCG AGGGGCGGAC TCTTGTCCCG GACGAGGTTC TTCGCAGCGT GCCAGACGAC 
TACCGGTGTG GGAAGGCACT GGGCCCCGTG GAGTCACTCC AGGGACACCT GCGGGACCCC 
ACCAAGATCC TCATCGGCTA CAGCCGAGGC CTGCTGGTCA TCTGGAACCA GGCGGCACAG 
TGCGCAGACC GCGTCTTCCT GGGTAACCAG CAGCTGGAGA GCCTGTGCTG GGAACGCAGC 
GGCAGCACGC TGGTCAGCTC CCACAGCGAC GGCAGCTACG CCGTCTGGTC CGCCGACGCC 
GGCGACTCCC CGATGACCCA GCCCACAGTG GCCACCACGC CATATGGCCC CTTCCCCTGC 
AAAGCCATTA ACAAGATTCT GTGGCGAAAC TGTGAATCTG GGGACCACTT CATCATCTTC 
AGTGGAGGCA TGCCCCGTGC CAGCTACGGT GACCGCCACT GTGTGAGTGT GCTCCGGGCC 
GAGACCCTGG TGACGCTGGA CTTCACTTCC CGCATCATCG ACTTCTTCAC GGTGCACAGC 
ACACGGCCTG AGGATGAGTT CGATGAGCCC CAGGCCCTGG CCGTGCTGCT CGAGGAGGAG 
CTGGTCGTGC TGGACCTGCA GACGCCCGGC TGGCCTGCCG TGCCCGCCCC GTACCTGGCC 
CCACTGCACT CATCCGCAAT CACCTGCTCT GCACACGTCG CCAATGTCCC TGCCAAGCTG 
TGGGCCCGCA TCGTGAGTGC TGGTGAGCAG CAGAGCCCGC AGCCTGCCTC CAGCTCCTCG 
AGCTGGCCCA TCACTGGGGG CCGGAACCTG GCCCAGGAGC CATCCCAGCG AGGGCTGCTG 
CTGACAGGCC ACGAGGACGG GACTGTGCGG TTCTGGGATG CCTCTGGCGT GGCACTGAGG 
CCGCTCTACA AGCTCAGCAC AGCCGGCCTC TTCCAGACAG ACTGTGAGCA CGCTGACAGC 
CTGGCCCAGG CCGCCGAGGA CGACTGGCCC CCCTTTCGCA AGGTGGGCTG CTTTGATCCC 
TACAGTGATG ACCCTCGGCT CGGTGTGCAG AAGGTGGCCC TGTGCAAGTA CACGGCCCAG 
ATGGTGGTGG CTGGCACGGC AGGCCAGGTG CTGGTGCTGG AGCTAAGTGA TGTGCCCTTG 
GAGCAGGCCG TCAGTGTGGC CAGCGTGGAC CTCCTCCAGG ACCGAGAGGG CTTCACGTGG 
AAGGGCCACG AGCGGCTGAG TCCGCGTACG GGGCCGCTGC TGTGGCCTGC TGGCTTCCAG 
CCCCGAGTGC TGGTTCAGTG CCTGCCACCA GCTGCTGTCA CCGCTGTCAC GCTCCATGCC 
GAGTGGAGCC TCGTGGCCTT TGGCACCAGT CACGGCTTTG GCCTCTTCGA CTATCAGCGC 
AGGAGCCCAG TGCTGGCCAG GTGCACGCTT CACCCCAATG ACTCTCTGGC CATGGAGGGC 
CCACTGTCCC GGGTGAAGTC CCTGAAGAAG TCTCTGCGCC AGTCCTTCCG GCGCATCCGC 
AAGAGCCGAG TCTCGGGCAA GAAGCGGGCT GCCAATGCCA CCAGCAAGTT GCAGGAGGCC 
AACGCGCAGC TGGCCGAGCA GGCGTGTCCC CACGACGTGG AGATGACCCC GGTGCAGCGC 
CGCATCGAGC CCCGCTCCGC CGACGACTCC CTCTCCGGCG TCGTCCGCTG CCTCTACTTT 
GCCGACACGT TCCTTCGAGA TGCGGCCCAC CACGGACCCA CCATGTGGGC GGGCACCAAC 
TCGGGCTCTG TGTTCGCCTA CGCGCTGGAG GTGCCGGCAG CGGCAGGCGG CGAGAAGCGA 
CCCGAGCGGG CGGTGGAGGC CGTGCTGGGC AAGGAGGTGC AGCTGATGCA CCGCGCCCCC 
GTGGTGGCCA TCGCCGTGCT GGATGGCCGT GGCCGCCCAC TGCCCGAGCC CTATGAGGCC 
TCCCGGGACC TGGCACAGGC CCCTGACATG CAGGGCGGCC ACGCCGTGCT CATCGCGTCT 
GAGGAGCAGT TCAAGGTGTT CACGCTGCCC AAGGTGAGCG CCAAGACCAA GTTCAAGCTG 
ACGGCCCACG AGGGCTGTCG CGTGCGCAAG GTGGCACTTG CCACGTTTGC CAGTGTGGCC 
TGTGAGGACT ACGCGGAGAC CTGCCTGGCC TGCCTCACCA ACCTGGGCGA CGTGCACGTG 
TTCTCAGTGC CTGGCCTGCG GCCGCAGGTG CACTACTCCT GTATCCGGAA GGAGGACATC 
AGCGGCATCG CTTCCTGCGT CTTCACGCGC CACGGCCAGG GTTTTTACTT GATTTCTCCG 
TCTGAATTTG AACGCTTCTC TCTAAGTGCC CGGAATATCA CAGAGCCGCT CTGCTCTCTG 
GACATCAGCT GGCCCCGAGA CAGCACCCGT GCCAGCCACA GGCTCCGAGA GTCACCCAAG 
CTGAGCCAGG CTAACGGGAC CCCAGGCATC GTCCTGGCCT CACGGAGCCG CGATGGAAGT 
CCAGATCCTG GCCACAGCAA GGGAGCTGAC ACCCCCGAGC CGCCTGAGGC CATGCTCTCG 
CCCATGTCCA TGGACTCGGC CACTAGTGCG GACACCACAC TGGACACGAC AGGGGACGTG 
ACGGTGGAGG ACGTGAAGGA TTTCCTGGGC TCTTCAGAGG AATCGGAAAA CCTGAGGAAC 
TTGGCAGAAG ATGAGGCTCG AGCCTGCGCC ATCCTGATCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022347130.1
CDS	1..3225
Translation	MDRRPSSPCSYTSLEAALGWASGGPGAACRCPCPPLPLQTVEHGFPNQPSALAFDPELRIMAIGTRSGAVKIYGAPGVEFTGLHQDTATVTQMHFLPGQGRLLTLLDDSSLHLWEIAHRNGCAHLEEALSFQPPSRPGFDGASGPLSLARVTVVLLVAAGDMAALGTEGGSIFFLDVPTLTLLEGRTLVPDEVLRSVPDDYRCGKALGPVESLQGHLRDPTKILIGYSRGLLVIWNQAAQCADRVFLGNQQLESLCWERSGSTLVSSHSDGSYAVWSADAGDSPMTQPTVATTPYGPFPCKAINKILWRNCESGDHFIIFSGGMPRASYGDRHCVSVLRAETLVTLDFTSRIIDFFTVHSTRPEDEFDEPQALAVLLEEELVVLDLQTPGWPAVPAPYLAPLHSSAITCSAHVANVPAKLWARIVSAGEQQSPQPASSSSSWPITGGRNLAQEPSQRGLLLTGHEDGTVRFWDASGVALRPLYKLSTAGLFQTDCEHADSLAQAAEDDWPPFRKVGCFDPYSDDPRLGVQKVALCKYTAQMVVAGTAGQVLVLELSDVPLEQAVSVASVDLLQDREGFTWKGHERLSPRTGPLLWPAGFQPRVLVQCLPPAAVTAVTLHAEWSLVAFGTSHGFGLFDYQRRSPVLARCTLHPNDSLAMEGPLSRVKSLKKSLRQSFRRIRKSRVSGKKRAANATSKLQEANAQLAEQACPHDVEMTPVQRRIEPRSADDSLSGVVRCLYFADTFLRDAAHHGPTMWAGTNSGSVFAYALEVPAAAGGEKRPERAVEAVLGKEVQLMHRAPVVAIAVLDGRGRPLPEPYEASRDLAQAPDMQGGHAVLIASEEQFKVFTLPKVSAKTKFKLTAHEGCRVRKVALATFASVACEDYAETCLACLTNLGDVHVFSVPGLRPQVHYSCIRKEDISGIASCVFTRHGQGFYLISPSEFERFSLSARNITEPLCSLDISWPRDSTRASHRLRESPKLSQANGTPGIVLASRSRDGSPDPGHSKGADTPEPPEAMLSPMSMDSATSADTTLDTTGDVTVEDVKDFLGSSEESENLRNLAEDEARACAILIK

Target ORF information:

RefSeq Version	XM_022491422.1
Organism	Enhydra lutris kenyoni
Definition	Enhydra lutris kenyoni LLGL1, scribble cell polarity complex component (LOC111139221), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022491422.1

ORF Insert Sequence:

ATGGACAGGC GCCCATCCTC ACCGTGCAGC TACACTAGTC TGGAGGCTGC CTTGGGGTGG 
GCCTCAGGTG GGCCAGGGGC TGCGTGCCGC TGCCCATGCC CTCCCCTCCC CCTGCAGACC 
GTGGAGCATG GCTTCCCCAA CCAGCCCAGC GCCCTGGCCT TTGACCCAGA GCTCCGCATC 
ATGGCCATCG GCACGAGGTC TGGGGCCGTC AAGATCTATG GTGCACCTGG TGTGGAGTTT 
ACCGGCCTGC ACCAGGACAC AGCCACTGTC ACCCAGATGC ACTTCCTGCC TGGCCAGGGC 
CGCCTCCTGA CCCTGTTGGA CGATAGCAGC CTGCACCTCT GGGAGATTGC CCACCGCAAC 
GGCTGCGCTC ACCTGGAGGA GGCTCTCAGC TTCCAGCCAC CGAGTCGGCC GGGCTTTGAT 
GGGGCCAGTG GCCCGCTCAG CCTTGCCCGC GTCACAGTGG TCCTGCTGGT GGCGGCTGGT 
GACATGGCAG CCCTGGGCAC TGAGGGCGGC AGCATCTTCT TCCTGGATGT TCCCACCCTG 
ACACTGCTCG AGGGGCGGAC TCTTGTCCCG GACGAGGTTC TTCGCAGCGT GCCAGACGAC 
TACCGGTGTG GGAAGGCACT GGGCCCCGTG GAGTCACTCC AGGGACACCT GCGGGACCCC 
ACCAAGATCC TCATCGGCTA CAGCCGAGGC CTGCTGGTCA TCTGGAACCA GGCGGCACAG 
TGCGCAGACC GCGTCTTCCT GGGTAACCAG CAGCTGGAGA GCCTGTGCTG GGAACGCAGC 
GGCAGCACGC TGGTCAGCTC CCACAGCGAC GGCAGCTACG CCGTCTGGTC CGCCGACGCC 
GGCGACTCCC CGATGACCCA GCCCACAGTG GCCACCACGC CATATGGCCC CTTCCCCTGC 
AAAGCCATTA ACAAGATTCT GTGGCGAAAC TGTGAATCTG GGGACCACTT CATCATCTTC 
AGTGGAGGCA TGCCCCGTGC CAGCTACGGT GACCGCCACT GTGTGAGTGT GCTCCGGGCC 
GAGACCCTGG TGACGCTGGA CTTCACTTCC CGCATCATCG ACTTCTTCAC GGTGCACAGC 
ACACGGCCTG AGGATGAGTT CGATGAGCCC CAGGCCCTGG CCGTGCTGCT CGAGGAGGAG 
CTGGTCGTGC TGGACCTGCA GACGCCCGGC TGGCCTGCCG TGCCCGCCCC GTACCTGGCC 
CCACTGCACT CATCCGCAAT CACCTGCTCT GCACACGTCG CCAATGTCCC TGCCAAGCTG 
TGGGCCCGCA TCGTGAGTGC TGGTGAGCAG CAGAGCCCGC AGCCTGCCTC CAGCTCCTCG 
AGCTGGCCCA TCACTGGGGG CCGGAACCTG GCCCAGGAGC CATCCCAGCG AGGGCTGCTG 
CTGACAGGCC ACGAGGACGG GACTGTGCGG TTCTGGGATG CCTCTGGCGT GGCACTGAGG 
CCGCTCTACA AGCTCAGCAC AGCCGGCCTC TTCCAGACAG ACTGTGAGCA CGCTGACAGC 
CTGGCCCAGG CCGCCGAGGA CGACTGGCCC CCCTTTCGCA AGGTGGGCTG CTTTGATCCC 
TACAGTGATG ACCCTCGGCT CGGTGTGCAG AAGGTGGCCC TGTGCAAGTA CACGGCCCAG 
ATGGTGGTGG CTGGCACGGC AGGCCAGGTG CTGGTGCTGG AGCTAAGTGA TGTGCCCTTG 
GAGCAGGCCG TCAGTGTGGC CAGCGTGGAC CTCCTCCAGG ACCGAGAGGG CTTCACGTGG 
AAGGGCCACG AGCGGCTGAG TCCGCGTACG GGGCCGCTGC TGTGGCCTGC TGGCTTCCAG 
CCCCGAGTGC TGGTTCAGTG CCTGCCACCA GCTGCTGTCA CCGCTGTCAC GCTCCATGCC 
GAGTGGAGCC TCGTGGCCTT TGGCACCAGT CACGGCTTTG GCCTCTTCGA CTATCAGCGC 
AGGAGCCCAG TGCTGGCCAG GTGCACGCTT CACCCCAATG ACTCTCTGGC CATGGAGGGC 
CCACTGTCCC GGGTGAAGTC CCTGAAGAAG TCTCTGCGCC AGTCCTTCCG GCGCATCCGC 
AAGAGCCGAG TCTCGGGCAA GAAGCGGGCT GCCAATGCCA CCAGCAAGTT GCAGGAGGCC 
AACGCGCAGC TGGCCGAGCA GGCGTGTCCC CACGACGTGG AGATGACCCC GGTGCAGCGC 
CGCATCGAGC CCCGCTCCGC CGACGACTCC CTCTCCGGCG TCGTCCGCTG CCTCTACTTT 
GCCGACACGT TCCTTCGAGA TGCGGCCCAC CACGGACCCA CCATGTGGGC GGGCACCAAC 
TCGGGCTCTG TGTTCGCCTA CGCGCTGGAG GTGCCGGCAG CGGCAGGCGG CGAGAAGCGA 
CCCGAGCGGG CGGTGGAGGC CGTGCTGGGC AAGGAGGTGC AGCTGATGCA CCGCGCCCCC 
GTGGTGGCCA TCGCCGTGCT GGATGGCCGT GGCCGCCCAC TGCCCGAGCC CTATGAGGCC 
TCCCGGGACC TGGCACAGGC CCCTGACATG CAGGGCGGCC ACGCCGTGCT CATCGCGTCT 
GAGGAGCAGT TCAAGGTGTT CACGCTGCCC AAGGTGAGCG CCAAGACCAA GTTCAAGCTG 
ACGGCCCACG AGGGCTGTCG CGTGCGCAAG GTGGCACTTG CCACGTTTGC CAGTGTGGCC 
TGTGAGGACT ACGCGGAGAC CTGCCTGGCC TGCCTCACCA ACCTGGGCGA CGTGCACGTG 
TTCTCAGTGC CTGGCCTGCG GCCGCAGGTG CACTACTCCT GTATCCGGAA GGAGGACATC 
AGCGGCATCG CTTCCTGCGT CTTCACGCGC CACGGCCAGG GTTTTTACTT GATTTCTCCG 
TCTGAATTTG AACGCTTCTC TCTAAGTGCC CGGAATATCA CAGAGCCGCT CTGCTCTCTG 
GACATCAGCT GGCCCCGAGA CAGCACCCGT GCCAGCCACA GGCTCCGAGA GTCACCCAAG 
CTGAGCCAGG CTAACGGGAC CCCAGGCATC GTCCTGGCCT CACGGAGCCG CGATGGAAGT 
CCAGATCCTG GCCACAGCAA GGGAGCTGAC ACCCCCGAGC CGCCTGAGGC CATGCTCTCG 
CCCATGTCCA TGGACTCGGC CACTAGTGCG GACACCACAC TGGACACGAC AGGGGACGTG 
ACGGTGGAGG ACGTGAAGGA TTTCCTGGGC TCTTCAGAGG AATCGGAAAA CCTGAGGAAC 
TTGGCAGAAG ATGAGGCTCG AGCCTGCGCC ATCCTGATCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC111139221 ( XM_022491423.1 ) cDNA ORF clone, Enhydra lutris kenyoni -> XP_022347131.1 Enhydra lutris kenyoni LLGL1, scribble cell polarity complex component (LOC111139221), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OEn54774
Clone ID Related Accession (Same CDS sequence)	XM_022491423.1
Accession Version	XM_022491423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-19
Organism	Enhydra lutris kenyoni
Product	lethal(2) giant larvae protein homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019154181.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Enhydra lutris kenyoni Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGT TTCGGTTCCG GCGGCAGGGC GCCGACCCGC AGCGCGAGAA GCTCAAGCAG 
GAGCTCTTTG CCTTCAACAA GACCGTGGAG CATGGCTTCC CCAACCAGCC CAGCGCCCTG 
GCCTTTGACC CAGAGCTCCG CATCATGGCC ATCGGCACGA GGTCTGGGGC CGTCAAGATC 
TATGGTGCAC CTGGTGTGGA GTTTACCGGC CTGCACCAGG ACACAGCCAC TGTCACCCAG 
ATGCACTTCC TGCCTGGCCA GGGCCGCCTC CTGACCCTGT TGGACGATAG CAGCCTGCAC 
CTCTGGGAGA TTGCCCACCG CAACGGCTGC GCTCACCTGG AGGAGGCTCT CAGCTTCCAG 
CCACCGAGTC GGCCGGGCTT TGATGGGGCC AGTGGCCCGC TCAGCCTTGC CCGCGTCACA 
GTGGTCCTGC TGGTGGCGGC TGGTGACATG GCAGCCCTGG GCACTGAGGG CGGCAGCATC 
TTCTTCCTGG ATGTTCCCAC CCTGACACTG CTCGAGGGGC GGACTCTTGT CCCGGACGAG 
GTTCTTCGCA GCGTGCCAGA CGACTACCGG TGTGGGAAGG CACTGGGCCC CGTGGAGTCA 
CTCCAGGGAC ACCTGCGGGA CCCCACCAAG ATCCTCATCG GCTACAGCCG AGGCCTGCTG 
GTCATCTGGA ACCAGGCGGC ACAGTGCGCA GACCGCGTCT TCCTGGGTAA CCAGCAGCTG 
GAGAGCCTGT GCTGGGAACG CAGCGGCAGC ACGCTGGTCA GCTCCCACAG CGACGGCAGC 
TACGCCGTCT GGTCCGCCGA CGCCGGCGAC TCCCCGATGA CCCAGCCCAC AGTGGCCACC 
ACGCCATATG GCCCCTTCCC CTGCAAAGCC ATTAACAAGA TTCTGTGGCG AAACTGTGAA 
TCTGGGGACC ACTTCATCAT CTTCAGTGGA GGCATGCCCC GTGCCAGCTA CGGTGACCGC 
CACTGTGTGA GTGTGCTCCG GGCCGAGACC CTGGTGACGC TGGACTTCAC TTCCCGCATC 
ATCGACTTCT TCACGGTGCA CAGCACACGG CCTGAGGATG AGTTCGATGA GCCCCAGGCC 
CTGGCCGTGC TGCTCGAGGA GGAGCTGGTC GTGCTGGACC TGCAGACGCC CGGCTGGCCT 
GCCGTGCCCG CCCCGTACCT GGCCCCACTG CACTCATCCG CAATCACCTG CTCTGCACAC 
GTCGCCAATG TCCCTGCCAA GCTGTGGGCC CGCATCGTGA GTGCTGGTGA GCAGCAGAGC 
CCGCAGCCTG CCTCCAGCTC CTCGAGCTGG CCCATCACTG GGGGCCGGAA CCTGGCCCAG 
GAGCCATCCC AGCGAGGGCT GCTGCTGACA GGCCACGAGG ACGGGACTGT GCGGTTCTGG 
GATGCCTCTG GCGTGGCACT GAGGCCGCTC TACAAGCTCA GCACAGCCGG CCTCTTCCAG 
ACAGACTGTG AGCACGCTGA CAGCCTGGCC CAGGCCGCCG AGGACGACTG GCCCCCCTTT 
CGCAAGGTGG GCTGCTTTGA TCCCTACAGT GATGACCCTC GGCTCGGTGT GCAGAAGGTG 
GCCCTGTGCA AGTACACGGC CCAGATGGTG GTGGCTGGCA CGGCAGGCCA GGTGCTGGTG 
CTGGAGCTAA GTGATGTGCC CTTGGAGCAG GCCGTCAGTG TGGCCAGCGT GGACCTCCTC 
CAGGACCGAG AGGGCTTCAC GTGGAAGGGC CACGAGCGGC TGAGTCCGCG TACGGGGCCG 
CTGCTGTGGC CTGCTGGCTT CCAGCCCCGA GTGCTGGTTC AGTGCCTGCC ACCAGCTGCT 
GTCACCGCTG TCACGCTCCA TGCCGAGTGG AGCCTCGTGG CCTTTGGCAC CAGTCACGGC 
TTTGGCCTCT TCGACTATCA GCGCAGGAGC CCAGTGCTGG CCAGGTGCAC GCTTCACCCC 
AATGACTCTC TGGCCATGGA GGGCCCACTG TCCCGGGTGA AGTCCCTGAA GAAGTCTCTG 
CGCCAGTCCT TCCGGCGCAT CCGCAAGAGC CGAGTCTCGG GCAAGAAGCG GGCTGCCAAT 
GCCACCAGCA AGTTGCAGGA GGCCAACGCG CAGCTGGCCG AGCAGGCGTG TCCCCACGAC 
GTGGAGATGA CCCCGGTGCA GCGCCGCATC GAGCCCCGCT CCGCCGACGA CTCCCTCTCC 
GGCGTCGTCC GCTGCCTCTA CTTTGCCGAC ACGTTCCTTC GAGATGCGGC CCACCACGGA 
CCCACCATGT GGGCGGGCAC CAACTCGGGC TCTGTGTTCG CCTACGCGCT GGAGGTGCCG 
GCAGCGGCAG GCGGCGAGAA GCGACCCGAG CGGGCGGTGG AGGCCGTGCT GGGCAAGGAG 
GTGCAGCTGA TGCACCGCGC CCCCGTGGTG GCCATCGCCG TGCTGGATGG CCGTGGCCGC 
CCACTGCCCG AGCCCTATGA GGCCTCCCGG GACCTGGCAC AGGCCCCTGA CATGCAGGGC 
GGCCACGCCG TGCTCATCGC GTCTGAGGAG CAGTTCAAGG TGTTCACGCT GCCCAAGGTG 
AGCGCCAAGA CCAAGTTCAA GCTGACGGCC CACGAGGGCT GTCGCGTGCG CAAGGTGGCA 
CTTGCCACGT TTGCCAGTGT GGCCTGTGAG GACTACGCGG AGACCTGCCT GGCCTGCCTC 
ACCAACCTGG GCGACGTGCA CGTGTTCTCA GTGCCTGGCC TGCGGCCGCA GGTGCACTAC 
TCCTGTATCC GGAAGGAGGA CATCAGCGGC ATCGCTTCCT GCGTCTTCAC GCGCCACGGC 
CAGGGTTTTT ACTTGATTTC TCCGTCTGAA TTTGAACGCT TCTCTCTAAG TGCCCGGAAT 
ATCACAGAGC CGCTCTGCTC TCTGGACATC AGCTGGCCCC GAGACAGCAC CCGTGCCAGC 
CACAGGCTCC GAGAGTCACC CAAGCTGAGC CAGGCTAACG GGACCCCAGG CATCGTCCTG 
GCCTCACGGA GCCGCGATGG AAGTCCAGAT CCTGGCCACA GCAAGGGAGC TGACACCCCC 
GAGCCGCCTG AGGCCATGCT CTCGCCCATG TCCATGGACT CGGCCACTAG TGCGGACACC 
ACACTGGACA CGACAGGGGA CGTGACGGTG GAGGACGTGA AGGATTTCCT GGGCTCTTCA 
GAGGAATCGG AAAACCTGAG GAACTTGGCA GAAGATGAGG CTCGAGCCTG CGCCATCCTG 
ATCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022347131.1
CDS	8..3196
Translation	MMKFRFRRQGADPQREKLKQELFAFNKTVEHGFPNQPSALAFDPELRIMAIGTRSGAVKIYGAPGVEFTGLHQDTATVTQMHFLPGQGRLLTLLDDSSLHLWEIAHRNGCAHLEEALSFQPPSRPGFDGASGPLSLARVTVVLLVAAGDMAALGTEGGSIFFLDVPTLTLLEGRTLVPDEVLRSVPDDYRCGKALGPVESLQGHLRDPTKILIGYSRGLLVIWNQAAQCADRVFLGNQQLESLCWERSGSTLVSSHSDGSYAVWSADAGDSPMTQPTVATTPYGPFPCKAINKILWRNCESGDHFIIFSGGMPRASYGDRHCVSVLRAETLVTLDFTSRIIDFFTVHSTRPEDEFDEPQALAVLLEEELVVLDLQTPGWPAVPAPYLAPLHSSAITCSAHVANVPAKLWARIVSAGEQQSPQPASSSSSWPITGGRNLAQEPSQRGLLLTGHEDGTVRFWDASGVALRPLYKLSTAGLFQTDCEHADSLAQAAEDDWPPFRKVGCFDPYSDDPRLGVQKVALCKYTAQMVVAGTAGQVLVLELSDVPLEQAVSVASVDLLQDREGFTWKGHERLSPRTGPLLWPAGFQPRVLVQCLPPAAVTAVTLHAEWSLVAFGTSHGFGLFDYQRRSPVLARCTLHPNDSLAMEGPLSRVKSLKKSLRQSFRRIRKSRVSGKKRAANATSKLQEANAQLAEQACPHDVEMTPVQRRIEPRSADDSLSGVVRCLYFADTFLRDAAHHGPTMWAGTNSGSVFAYALEVPAAAGGEKRPERAVEAVLGKEVQLMHRAPVVAIAVLDGRGRPLPEPYEASRDLAQAPDMQGGHAVLIASEEQFKVFTLPKVSAKTKFKLTAHEGCRVRKVALATFASVACEDYAETCLACLTNLGDVHVFSVPGLRPQVHYSCIRKEDISGIASCVFTRHGQGFYLISPSEFERFSLSARNITEPLCSLDISWPRDSTRASHRLRESPKLSQANGTPGIVLASRSRDGSPDPGHSKGADTPEPPEAMLSPMSMDSATSADTTLDTTGDVTVEDVKDFLGSSEESENLRNLAEDEARACAILIK

Target ORF information:

RefSeq Version	XM_022491423.1
Organism	Enhydra lutris kenyoni
Definition	Enhydra lutris kenyoni LLGL1, scribble cell polarity complex component (LOC111139221), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022491423.1

ORF Insert Sequence:

ATGATGAAGT TTCGGTTCCG GCGGCAGGGC GCCGACCCGC AGCGCGAGAA GCTCAAGCAG 
GAGCTCTTTG CCTTCAACAA GACCGTGGAG CATGGCTTCC CCAACCAGCC CAGCGCCCTG 
GCCTTTGACC CAGAGCTCCG CATCATGGCC ATCGGCACGA GGTCTGGGGC CGTCAAGATC 
TATGGTGCAC CTGGTGTGGA GTTTACCGGC CTGCACCAGG ACACAGCCAC TGTCACCCAG 
ATGCACTTCC TGCCTGGCCA GGGCCGCCTC CTGACCCTGT TGGACGATAG CAGCCTGCAC 
CTCTGGGAGA TTGCCCACCG CAACGGCTGC GCTCACCTGG AGGAGGCTCT CAGCTTCCAG 
CCACCGAGTC GGCCGGGCTT TGATGGGGCC AGTGGCCCGC TCAGCCTTGC CCGCGTCACA 
GTGGTCCTGC TGGTGGCGGC TGGTGACATG GCAGCCCTGG GCACTGAGGG CGGCAGCATC 
TTCTTCCTGG ATGTTCCCAC CCTGACACTG CTCGAGGGGC GGACTCTTGT CCCGGACGAG 
GTTCTTCGCA GCGTGCCAGA CGACTACCGG TGTGGGAAGG CACTGGGCCC CGTGGAGTCA 
CTCCAGGGAC ACCTGCGGGA CCCCACCAAG ATCCTCATCG GCTACAGCCG AGGCCTGCTG 
GTCATCTGGA ACCAGGCGGC ACAGTGCGCA GACCGCGTCT TCCTGGGTAA CCAGCAGCTG 
GAGAGCCTGT GCTGGGAACG CAGCGGCAGC ACGCTGGTCA GCTCCCACAG CGACGGCAGC 
TACGCCGTCT GGTCCGCCGA CGCCGGCGAC TCCCCGATGA CCCAGCCCAC AGTGGCCACC 
ACGCCATATG GCCCCTTCCC CTGCAAAGCC ATTAACAAGA TTCTGTGGCG AAACTGTGAA 
TCTGGGGACC ACTTCATCAT CTTCAGTGGA GGCATGCCCC GTGCCAGCTA CGGTGACCGC 
CACTGTGTGA GTGTGCTCCG GGCCGAGACC CTGGTGACGC TGGACTTCAC TTCCCGCATC 
ATCGACTTCT TCACGGTGCA CAGCACACGG CCTGAGGATG AGTTCGATGA GCCCCAGGCC 
CTGGCCGTGC TGCTCGAGGA GGAGCTGGTC GTGCTGGACC TGCAGACGCC CGGCTGGCCT 
GCCGTGCCCG CCCCGTACCT GGCCCCACTG CACTCATCCG CAATCACCTG CTCTGCACAC 
GTCGCCAATG TCCCTGCCAA GCTGTGGGCC CGCATCGTGA GTGCTGGTGA GCAGCAGAGC 
CCGCAGCCTG CCTCCAGCTC CTCGAGCTGG CCCATCACTG GGGGCCGGAA CCTGGCCCAG 
GAGCCATCCC AGCGAGGGCT GCTGCTGACA GGCCACGAGG ACGGGACTGT GCGGTTCTGG 
GATGCCTCTG GCGTGGCACT GAGGCCGCTC TACAAGCTCA GCACAGCCGG CCTCTTCCAG 
ACAGACTGTG AGCACGCTGA CAGCCTGGCC CAGGCCGCCG AGGACGACTG GCCCCCCTTT 
CGCAAGGTGG GCTGCTTTGA TCCCTACAGT GATGACCCTC GGCTCGGTGT GCAGAAGGTG 
GCCCTGTGCA AGTACACGGC CCAGATGGTG GTGGCTGGCA CGGCAGGCCA GGTGCTGGTG 
CTGGAGCTAA GTGATGTGCC CTTGGAGCAG GCCGTCAGTG TGGCCAGCGT GGACCTCCTC 
CAGGACCGAG AGGGCTTCAC GTGGAAGGGC CACGAGCGGC TGAGTCCGCG TACGGGGCCG 
CTGCTGTGGC CTGCTGGCTT CCAGCCCCGA GTGCTGGTTC AGTGCCTGCC ACCAGCTGCT 
GTCACCGCTG TCACGCTCCA TGCCGAGTGG AGCCTCGTGG CCTTTGGCAC CAGTCACGGC 
TTTGGCCTCT TCGACTATCA GCGCAGGAGC CCAGTGCTGG CCAGGTGCAC GCTTCACCCC 
AATGACTCTC TGGCCATGGA GGGCCCACTG TCCCGGGTGA AGTCCCTGAA GAAGTCTCTG 
CGCCAGTCCT TCCGGCGCAT CCGCAAGAGC CGAGTCTCGG GCAAGAAGCG GGCTGCCAAT 
GCCACCAGCA AGTTGCAGGA GGCCAACGCG CAGCTGGCCG AGCAGGCGTG TCCCCACGAC 
GTGGAGATGA CCCCGGTGCA GCGCCGCATC GAGCCCCGCT CCGCCGACGA CTCCCTCTCC 
GGCGTCGTCC GCTGCCTCTA CTTTGCCGAC ACGTTCCTTC GAGATGCGGC CCACCACGGA 
CCCACCATGT GGGCGGGCAC CAACTCGGGC TCTGTGTTCG CCTACGCGCT GGAGGTGCCG 
GCAGCGGCAG GCGGCGAGAA GCGACCCGAG CGGGCGGTGG AGGCCGTGCT GGGCAAGGAG 
GTGCAGCTGA TGCACCGCGC CCCCGTGGTG GCCATCGCCG TGCTGGATGG CCGTGGCCGC 
CCACTGCCCG AGCCCTATGA GGCCTCCCGG GACCTGGCAC AGGCCCCTGA CATGCAGGGC 
GGCCACGCCG TGCTCATCGC GTCTGAGGAG CAGTTCAAGG TGTTCACGCT GCCCAAGGTG 
AGCGCCAAGA CCAAGTTCAA GCTGACGGCC CACGAGGGCT GTCGCGTGCG CAAGGTGGCA 
CTTGCCACGT TTGCCAGTGT GGCCTGTGAG GACTACGCGG AGACCTGCCT GGCCTGCCTC 
ACCAACCTGG GCGACGTGCA CGTGTTCTCA GTGCCTGGCC TGCGGCCGCA GGTGCACTAC 
TCCTGTATCC GGAAGGAGGA CATCAGCGGC ATCGCTTCCT GCGTCTTCAC GCGCCACGGC 
CAGGGTTTTT ACTTGATTTC TCCGTCTGAA TTTGAACGCT TCTCTCTAAG TGCCCGGAAT 
ATCACAGAGC CGCTCTGCTC TCTGGACATC AGCTGGCCCC GAGACAGCAC CCGTGCCAGC 
CACAGGCTCC GAGAGTCACC CAAGCTGAGC CAGGCTAACG GGACCCCAGG CATCGTCCTG 
GCCTCACGGA GCCGCGATGG AAGTCCAGAT CCTGGCCACA GCAAGGGAGC TGACACCCCC 
GAGCCGCCTG AGGCCATGCT CTCGCCCATG TCCATGGACT CGGCCACTAG TGCGGACACC 
ACACTGGACA CGACAGGGGA CGTGACGGTG GAGGACGTGA AGGATTTCCT GGGCTCTTCA 
GAGGAATCGG AAAACCTGAG GAACTTGGCA GAAGATGAGG CTCGAGCCTG CGCCATCCTG 
ATCAAATAA