IL12B cDNA ORF clone,

Gene
Clones
gRNAs

The following IL12B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IL12B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OWi51679	XM_027902713.1 Latest version!	Empidonax traillii interleukin 12B (IL12B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IL12B ( XM_027902713.1 ) cDNA ORF clone, -> XP_027758514.1 Empidonax traillii interleukin 12B (IL12B), mRNA.

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CloneID	OWi51679
Clone ID Related Accession (Same CDS sequence)	XM_027902713.1
Accession Version	XM_027902713.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-23
Organism	Empidonax traillii(willow flycatcher)
Product	interleukin-12 subunit beta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020955215.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Empidonax traillii Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCACC TGCTCTGTGC CTTATTTTCA CTGTTTTCCT TTGCTGCCCT ACTGGAAGCT 
GTGCAGTGGA AACTTCAGGA AAATGTGTAT GTCATAGAAT CCGAGTGGAA TGCTGAGACA 
CCAGCGGCGA GAGTGGAGCT CACCTGTAAC ACACCTAATG AAGCCGTTTA CTGGAAAAAG 
GGCTCTGAAT GGAAAGGGAA AGGAAAGACT CTGATTGTTG AAGTGAAGGA GTTCCCTGAT 
GCTGGAAACT ACACCTGTCT GACAGAGAAT ACCCATGAAA TCCTCAGCTA TAATTTCCTC 
CTCATAAGCA AAATCGACTC CAACGGACAA ATGATAAGGT CGATTCTGAA AAGCTTTACA 
GAGCCATTGC AGACCTTTAT AAAATGTGAG GCAAAGAACT ACTCTGGAAT TTTCATGTGT 
TCATGGATGA CAGAAAATGA GAGTCCAAAT ATGAAGTTCA CCATCAGAGG TTTAAAAGGC 
TCTCAGGGAG ATGTGACCTG CAGCAGCCCC GTGGCTCACA CTGATGGATT GGTGACCAAA 
TACACGGCCC AGTGCCGCAA AGAAAACTAC TGTCCCTTTG CTGAAGAGCA TCAGCCCATT 
TATGTGTTCC TCGAGGTCAT CGACGAGGTG GAATATGAGA ACTACACCAG CAGCTTCTTC 
ATCAGAGATA TCATAAAACC TGACCCACCC CAGTGTCAGT ATGTGGCCAC AAATGGAATG 
GTGACCTGGA CATATCCCAA AACATGGAGC ACCCCAAAGT CCTACTTCCC TTTGACTTTT 
AAGGTCAAAG TTAAAAGCCC AAGGAAACGC AGACCTCAGG TCTATGATAC CGATGAACAG 
TCAATCCACC TTCCAAACAT TGAGCCAAAA GATGAGATCT CCGTGCAGGC CAGGGATCGT 
TATTACCACT CATCCTGGAG TGACTGGTCT TCACTCTGCA GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027758514.1
CDS	1..945
Translation	MPHLLCALFSLFSFAALLEAVQWKLQENVYVIESEWNAETPAARVELTCNTPNEAVYWKKGSEWKGKGKTLIVEVKEFPDAGNYTCLTENTHEILSYNFLLISKIDSNGQMIRSILKSFTEPLQTFIKCEAKNYSGIFMCSWMTENESPNMKFTIRGLKGSQGDVTCSSPVAHTDGLVTKYTAQCRKENYCPFAEEHQPIYVFLEVIDEVEYENYTSSFFIRDIIKPDPPQCQYVATNGMVTWTYPKTWSTPKSYFPLTFKVKVKSPRKRRPQVYDTDEQSIHLPNIEPKDEISVQARDRYYHSSWSDWSSLCR

Target ORF information:

RefSeq Version	XM_027902713.1
Organism	Empidonax traillii(willow flycatcher)
Definition	Empidonax traillii interleukin 12B (IL12B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027902713.1

ORF Insert Sequence:

ATGCCTCACC TGCTCTGTGC CTTATTTTCA CTGTTTTCCT TTGCTGCCCT ACTGGAAGCT 
GTGCAGTGGA AACTTCAGGA AAATGTGTAT GTCATAGAAT CCGAGTGGAA TGCTGAGACA 
CCAGCGGCGA GAGTGGAGCT CACCTGTAAC ACACCTAATG AAGCCGTTTA CTGGAAAAAG 
GGCTCTGAAT GGAAAGGGAA AGGAAAGACT CTGATTGTTG AAGTGAAGGA GTTCCCTGAT 
GCTGGAAACT ACACCTGTCT GACAGAGAAT ACCCATGAAA TCCTCAGCTA TAATTTCCTC 
CTCATAAGCA AAATCGACTC CAACGGACAA ATGATAAGGT CGATTCTGAA AAGCTTTACA 
GAGCCATTGC AGACCTTTAT AAAATGTGAG GCAAAGAACT ACTCTGGAAT TTTCATGTGT 
TCATGGATGA CAGAAAATGA GAGTCCAAAT ATGAAGTTCA CCATCAGAGG TTTAAAAGGC 
TCTCAGGGAG ATGTGACCTG CAGCAGCCCC GTGGCTCACA CTGATGGATT GGTGACCAAA 
TACACGGCCC AGTGCCGCAA AGAAAACTAC TGTCCCTTTG CTGAAGAGCA TCAGCCCATT 
TATGTGTTCC TCGAGGTCAT CGACGAGGTG GAATATGAGA ACTACACCAG CAGCTTCTTC 
ATCAGAGATA TCATAAAACC TGACCCACCC CAGTGTCAGT ATGTGGCCAC AAATGGAATG 
GTGACCTGGA CATATCCCAA AACATGGAGC ACCCCAAAGT CCTACTTCCC TTTGACTTTT 
AAGGTCAAAG TTAAAAGCCC AAGGAAACGC AGACCTCAGG TCTATGATAC CGATGAACAG 
TCAATCCACC TTCCAAACAT TGAGCCAAAA GATGAGATCT CCGTGCAGGC CAGGGATCGT 
TATTACCACT CATCCTGGAG TGACTGGTCT TCACTCTGCA GGTAA