NSMCE2 cDNA ORF clone, Egretta garzetta(little egret)

Gene
Clones
gRNAs

Summary

Gene Symbol	NSMCE2
Entrez Gene ID	104134440
Full Name	NSE2/MMS21 homolog, SMC5-SMC6 complex SUMO ligase
Gene Type	protein-coding
Organism	Egretta garzetta(little egret)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009647977.1	XP_009646272.1	E3 SUMO-protein ligase NSE2

Pathways from BioSystems
Gene Ontology

The following NSMCE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NSMCE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEg01678	XM_009647977.1 Latest version!	Egretta garzetta non-SMC element 2, MMS21 homolog (S. cerevisiae) (NSMCE2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NSMCE2 ( XM_009647977.1 ) cDNA ORF clone, Egretta garzetta(little egret) -> XP_009646272.1 Egretta garzetta non-SMC element 2, MMS21 homolog (S. cerevisiae) (NSMCE2), mRNA.

Price & Availability↑

CloneID	OEg01678
Clone ID Related Accession (Same CDS sequence)	XM_009647977.1
Accession Version	XM_009647977.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-15
Organism	Egretta garzetta(little egret)
Product	E3 SUMO-protein ligase NSE2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009258613.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Egretta garzetta Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGCG GTGCAAGGAT CTCATTCAAC TGTGTGGATT CCTCTCTTTC ATCTCTGAAA 
AACTGCCAGT CTTACATAAA TACTGGAATG GATATTGCTA CTCACGTTGC CCTTGACCTT 
GTGGAAAGTT TCAATGATGT AGAGGATGTT GACAGTATGG AAAATGTGAT GTTAGAATAT 
GCCGCAATGG ACAGAGAACT TAACCATTAC ATGAGAGCAA TTGAAGAAAC GGTAAATCAG 
ATAAAACGAG AAAAACCAGA AAATATACCA GATCTAAAAA ATTTAGTAAA AGAAAAATTT 
ACTGCACTGG AGAGCAAGAA CAGTGACTCA GATTTGCAAA GGAATGAAAA ATACATGTAT 
TTTAAGGATC AACTTAAGGA GATGAGAAAA CAATTTTGTC ATCAATCAGA TAATGGTAAT 
GAGGCCATTG AACAAATCGA TGAAGACATC GCCGTGACTC AGAGTCAGAT GAACTTTATT 
TGCCCCATTA CGCAGATGGA AATGAAGAGG CCGGTTCGAA ACAAAGTCTG TGGACATACT 
TATGAAGAAG ACGCCATTCT AAGAATTATC CAGAATCGAA AGCAGCAAAA GAAAAAAGTC 
CGATGCCCTA AAATTGGCTG TAGCCATGCT GATGTAAAAG GATCAGACCT TGTGCCAGAT 
GAAGTGCTTA AAAGAGCGAT TGACAGTCAG AATAAACAAA GCTGGTCGAC ACTGGACAAG 
TCAGCTGCTG CCACAAACAA AATTTGTTGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009646272.1
CDS	14..766
Translation	MPGGARISFNCVDSSLSSLKNCQSYINTGMDIATHVALDLVESFNDVEDVDSMENVMLEYAAMDRELNHYMRAIEETVNQIKREKPENIPDLKNLVKEKFTALESKNSDSDLQRNEKYMYFKDQLKEMRKQFCHQSDNGNEAIEQIDEDIAVTQSQMNFICPITQMEMKRPVRNKVCGHTYEEDAILRIIQNRKQQKKKVRCPKIGCSHADVKGSDLVPDEVLKRAIDSQNKQSWSTLDKSAAATNKICC

Target ORF information:

RefSeq Version	XM_009647977.1
Organism	Egretta garzetta(little egret)
Definition	Egretta garzetta non-SMC element 2, MMS21 homolog (S. cerevisiae) (NSMCE2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009647977.1

ORF Insert Sequence:

ATGCCAGGCG GTGCAAGGAT CTCATTCAAC TGTGTGGATT CCTCTCTTTC ATCTCTGAAA 
AACTGCCAGT CTTACATAAA TACTGGAATG GATATTGCTA CTCACGTTGC CCTTGACCTT 
GTGGAAAGTT TCAATGATGT AGAGGATGTT GACAGTATGG AAAATGTGAT GTTAGAATAT 
GCCGCAATGG ACAGAGAACT TAACCATTAC ATGAGAGCAA TTGAAGAAAC GGTAAATCAG 
ATAAAACGAG AAAAACCAGA AAATATACCA GATCTAAAAA ATTTAGTAAA AGAAAAATTT 
ACTGCACTGG AGAGCAAGAA CAGTGACTCA GATTTGCAAA GGAATGAAAA ATACATGTAT 
TTTAAGGATC AACTTAAGGA GATGAGAAAA CAATTTTGTC ATCAATCAGA TAATGGTAAT 
GAGGCCATTG AACAAATCGA TGAAGACATC GCCGTGACTC AGAGTCAGAT GAACTTTATT 
TGCCCCATTA CGCAGATGGA AATGAAGAGG CCGGTTCGAA ACAAAGTCTG TGGACATACT 
TATGAAGAAG ACGCCATTCT AAGAATTATC CAGAATCGAA AGCAGCAAAA GAAAAAAGTC 
CGATGCCCTA AAATTGGCTG TAGCCATGCT GATGTAAAAG GATCAGACCT TGTGCCAGAT 
GAAGTGCTTA AAAGAGCGAT TGACAGTCAG AATAAACAAA GCTGGTCGAC ACTGGACAAG 
TCAGCTGCTG CCACAAACAA AATTTGTTGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.