EIF4ENIF1 cDNA ORF clone, Echinops telfairi(small Madagascar hedgehog)

Gene
Clones
gRNAs

Summary

Gene Symbol	EIF4ENIF1
Entrez Gene ID	101644748
Full Name	eukaryotic translation initiation factor 4E nuclear import factor 1
Gene Type	protein-coding
Organism	Echinops telfairi(small Madagascar hedgehog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004716705.2	XP_004716762.1	eukaryotic translation initiation factor 4E transporter isoform X1
XM_004716706.1	XP_004716763.1	eukaryotic translation initiation factor 4E transporter isoform X2

Pathways from BioSystems
Gene Ontology

The following EIF4ENIF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF4ENIF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEc23830	XM_004716705.2 Latest version!	Echinops telfairi eukaryotic translation initiation factor 4E nuclear import factor 1 (EIF4ENIF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OEc23831	XM_004716706.1 Latest version!	Echinops telfairi eukaryotic translation initiation factor 4E nuclear import factor 1 (EIF4ENIF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EIF4ENIF1 ( XM_004716705.2 ) cDNA ORF clone, Echinops telfairi(small Madagascar hedgehog) -> XP_004716762.1 Echinops telfairi eukaryotic translation initiation factor 4E nuclear import factor 1 (EIF4ENIF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OEc23830
Clone ID Related Accession (Same CDS sequence)	XM_004716705.2
Accession Version	XM_004716705.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-18
Organism	Echinops telfairi(small Madagascar hedgehog)
Product	eukaryotic translation initiation factor 4E transporter isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022103926.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 25, 2015 this sequence version replaced XM_004716705.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Echinops telfairi Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAGGA GAAGCGCGGG TGAAGCTGAG AATGGAGACG CTTTCCTTGA CCTGAAGAAG 
CTGCCAACCT CCAAATCCCC CCATCGCTAC ACAAAAGAGG AGCTTTTGGA TATAAAAGAG 
CGGCCCCATT CCAAACAGAG ACCTCCATGC CTCTCTGAAA AATATGACAG TGATGGCGTC 
TGGGACCCTG AGAAGTGGCA TGCCTCTCTC TACCCAGTTT CAGGGCGGAG CTCACCTGTG 
GAAAACCAGA AAAAAGAGCT GGATACAGAC CGGCCTTCCC TAGTGCGTCG GATAGTAGAT 
CCACGGGAGC GTGTGAAGGA AGACGATTTG GATGTTGTTC TCAGTCCACA GAGACGAAGC 
TTTGGTGGTG GCTGCCATGT GACGGCTGCT GTAAGCTCCC GACGCTCGGG GAGCCCACTG 
GAGAAAGACA GTGATGGGCT TCGTCTGCTT GGCGGACGGA GGATCGGCAG TGGGAGAATA 
ATGTCTGCCC GGACCTTTGA AAAGGACCAC CGTCTTAGTG ATAAGGACCT TCGGGACTTG 
AGAGACAGAG ACCGTGAAAG GGACTATAAG GACAAGCGTT TCAGGAGAGA GTTTGGGGAT 
AGTAAGCGTG TCTTTGGCGA GCGTAGAAGA AATGATTCTT ACACGGAAGA AGAACCAGAG 
TGGTTCTCAG CTGGACCCAC GAGCCAGTCT GAAACCATTG AGCTGACTGG CTTTGATGAC 
AAGATCCTAG AAGAAGACCA CAAAGGGAGA AAGAGAACGA GGCGGCGGAC AGCTTCTGTG 
AAGGAAGGCA TAGTCGAGTG CAATGGAGGA GTGGCCGAAG AGGAGGAGGT GGAGGTCATT 
CTTGCACAGG AGCCTGCTGC TGATCAGGAA GTACCGAGGG ACGCCGTCTT GCCTGAGCAG 
TCCCCAGGAG AATTTGACTT TAATGAGTTC TTTAACCTTG ATAAGGTGCC GTGCTTGGCT 
TCGATGATAG AAGATGTTCT AGGGGAAGGG TCCGTCTCGG CCAGCCGCTT TAGTAGGTGG 
TTTTCTAACC CCAGCCGATC AGGAAGCCGG TCCAGCAGTC TTGGGTCAAC ACCACATGAA 
GAACTGGAGA GACTTGCAGG TCTGGAGCAG GTCATCCTCT CTCCTGCACA GAACTCGGGG 
AATTACTTTG CTCCTATACC ATCAGAAGAT CATGCTGAAA ATAAAGTGGA TATTTTAGAA 
ATGCTACAGA AAGCCAAAGT GGACTTAAAA CCTCTTCTCT CCAGCCTTTC TGCAAATAAA 
GAAAAGCTTA AAGAAAGCTC ACATTCAGGG GTTGTGCTCT CAGTGGAAGA GGTAGAAGCA 
GGGCTCAAGG GCTTGAAGGT GGACCAGCAC ATGAAGAATT CAACTCCTTT CATGGCAGAA 
CATTTAGAGG AGACCCTGAG TGCTGTAACA AGCAACCGGC AGCTCAGAAA GGACGGCGAT 
ATGACTGCAT TCAACAAGCT AGTGAGCAGC ATGAAAGCAA GCGGGACTCT GCCCTCGCAG 
CCCAAAGCCA GTCGAAACCT TGAAAGCCAT TTGATGTCCC CTGCTGAGCT TCCAGGCCAG 
GCTGTCCCTA AGAACATCCT CCAGGAACTT CTGGGTCCAC CAGTTCAGAG ACCGGCTTCT 
TCTAATCTTC TGAGTGGCCT GATGGGGAGC TTGGAGCCTA CAGCATCTTT ACTGGGCCAA 
AGGGCACCCT CTCCTCCCTT GTCACAGGCA TTTCAAACTC GAGCAGCCTC GGCAGACTAC 
CTTCGCCCTA GAATATCTTC ACCAGTGGGT TTCCCACCAG GACCACCTCC GCAGCTTCTT 
GGGGACCCGT TCCAAGGCAT GCGCAAGCCC ATGAGCCCCA TCACAGTCCA GATGAGCCAG 
TTGGAGTTGC AACAGGCCGC TCTGGAGGGG CTGGCCTTGC CACATGACCT TGCAATGCAG 
ACAGCAAACT TCTACCAGCC TGGTTTTGGC AAACCACAAG TGGACAGAAC CAGAGATGGA 
TACAGAAACA GGCAACAGCG AGTGACCAAG TCGCCAGCAA ACATGCATCA AGGGAATTCC 
TCTTCCCCTG CTCCCTCGGC CTCCGTCACA AGCATGCTTT CTCCCTCCTT CACCCCTACC 
TCGGTGATTC GAAAGATGTT CGAGAGCAAA GAGAAAAGCA AGGAGGAGCC TGCCTCTGGG 
AAAGCCGCTC TTGGTGACCA TAAAGAGGGC ACTCAGGAGG CCAGTGAAGA CAACCTGCTG 
TCATCCAACC CCATGTCCAG TGCTGATCGA GACTCTTCTC CCACGACAAA TCCCAAGATG 
TCAACGTTGC AGCGGTCTTC CTGCTCTACC CCTCTGTCCC AAACCAACCG TTACACCAAA 
GAACAAGATT ATCGACCTAA AGCAGCCGGG AGGAAAACAC CCACCTTGGC CTCCCCAGTT 
CCAGGAGCAC CTTTTCTCCG CCCTGTCCAC CAAGTCCCCC TGGTCCCCCA TGTACCAATC 
GTTCGACCTG CTCACCAGCT TCACCCAGGC CTGGTCCAAA GGATGCATAT TCAACAGCAT 
CTTCCAAGTT TGCTCCAAGC TGGTATGCTT CCACCAGGGA TGGACCTGAC GCATTTACAG 
GGTATATCTG GCCCAATCCT GGGTCAGCCT TTTTACCCTT TACCCGCTGC TAATCACCCT 
CTACTAAACC CTCGTCCCGG GACACCTCTG CATCTGGCAA TGATGCAGCA GCAGTTACAG 
CGCTCAGTTC TGCATCCTCC AGGCTCCGGG TCTCAAGCAG CTGCTGTTAG CGTTCCCGTA 
ACCCCCCAGA ATGTGCCCAG CAGGTCGGGC CTGCCCCACG TGCACCCCCA GCTGGAGCAC 
CGCCCTAGCC AGAGGAGCAG CTCTCCGGTG GGCCTTGCCA AGTGGTTTGG CTCAGATGTG 
CTGCAGCAAC CCTTGCCTTC TATGCCTGCC AAAGTCATCA GTGTAGACGA GCTGGAGTAC 
CGACCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004716762.1
CDS	22..2970
Translation	MDRRSAGEAENGDAFLDLKKLPTSKSPHRYTKEELLDIKERPHSKQRPPCLSEKYDSDGVWDPEKWHASLYPVSGRSSPVENQKKELDTDRPSLVRRIVDPRERVKEDDLDVVLSPQRRSFGGGCHVTAAVSSRRSGSPLEKDSDGLRLLGGRRIGSGRIMSARTFEKDHRLSDKDLRDLRDRDRERDYKDKRFRREFGDSKRVFGERRRNDSYTEEEPEWFSAGPTSQSETIELTGFDDKILEEDHKGRKRTRRRTASVKEGIVECNGGVAEEEEVEVILAQEPAADQEVPRDAVLPEQSPGEFDFNEFFNLDKVPCLASMIEDVLGEGSVSASRFSRWFSNPSRSGSRSSSLGSTPHEELERLAGLEQVILSPAQNSGNYFAPIPSEDHAENKVDILEMLQKAKVDLKPLLSSLSANKEKLKESSHSGVVLSVEEVEAGLKGLKVDQHMKNSTPFMAEHLEETLSAVTSNRQLRKDGDMTAFNKLVSSMKASGTLPSQPKASRNLESHLMSPAELPGQAVPKNILQELLGPPVQRPASSNLLSGLMGSLEPTASLLGQRAPSPPLSQAFQTRAASADYLRPRISSPVGFPPGPPPQLLGDPFQGMRKPMSPITVQMSQLELQQAALEGLALPHDLAMQTANFYQPGFGKPQVDRTRDGYRNRQQRVTKSPANMHQGNSSSPAPSASVTSMLSPSFTPTSVIRKMFESKEKSKEEPASGKAALGDHKEGTQEASEDNLLSSNPMSSADRDSSPTTNPKMSTLQRSSCSTPLSQTNRYTKEQDYRPKAAGRKTPTLASPVPGAPFLRPVHQVPLVPHVPIVRPAHQLHPGLVQRMHIQQHLPSLLQAGMLPPGMDLTHLQGISGPILGQPFYPLPAANHPLLNPRPGTPLHLAMMQQQLQRSVLHPPGSGSQAAAVSVPVTPQNVPSRSGLPHVHPQLEHRPSQRSSSPVGLAKWFGSDVLQQPLPSMPAKVISVDELEYRP

Target ORF information:

RefSeq Version	XM_004716705.2
Organism	Echinops telfairi(small Madagascar hedgehog)
Definition	Echinops telfairi eukaryotic translation initiation factor 4E nuclear import factor 1 (EIF4ENIF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004716705.2

ORF Insert Sequence:

ATGGATAGGA GAAGCGCGGG TGAAGCTGAG AATGGAGACG CTTTCCTTGA CCTGAAGAAG 
CTGCCAACCT CCAAATCCCC CCATCGCTAC ACAAAAGAGG AGCTTTTGGA TATAAAAGAG 
CGGCCCCATT CCAAACAGAG ACCTCCATGC CTCTCTGAAA AATATGACAG TGATGGCGTC 
TGGGACCCTG AGAAGTGGCA TGCCTCTCTC TACCCAGTTT CAGGGCGGAG CTCACCTGTG 
GAAAACCAGA AAAAAGAGCT GGATACAGAC CGGCCTTCCC TAGTGCGTCG GATAGTAGAT 
CCACGGGAGC GTGTGAAGGA AGACGATTTG GATGTTGTTC TCAGTCCACA GAGACGAAGC 
TTTGGTGGTG GCTGCCATGT GACGGCTGCT GTAAGCTCCC GACGCTCGGG GAGCCCACTG 
GAGAAAGACA GTGATGGGCT TCGTCTGCTT GGCGGACGGA GGATCGGCAG TGGGAGAATA 
ATGTCTGCCC GGACCTTTGA AAAGGACCAC CGTCTTAGTG ATAAGGACCT TCGGGACTTG 
AGAGACAGAG ACCGTGAAAG GGACTATAAG GACAAGCGTT TCAGGAGAGA GTTTGGGGAT 
AGTAAGCGTG TCTTTGGCGA GCGTAGAAGA AATGATTCTT ACACGGAAGA AGAACCAGAG 
TGGTTCTCAG CTGGACCCAC GAGCCAGTCT GAAACCATTG AGCTGACTGG CTTTGATGAC 
AAGATCCTAG AAGAAGACCA CAAAGGGAGA AAGAGAACGA GGCGGCGGAC AGCTTCTGTG 
AAGGAAGGCA TAGTCGAGTG CAATGGAGGA GTGGCCGAAG AGGAGGAGGT GGAGGTCATT 
CTTGCACAGG AGCCTGCTGC TGATCAGGAA GTACCGAGGG ACGCCGTCTT GCCTGAGCAG 
TCCCCAGGAG AATTTGACTT TAATGAGTTC TTTAACCTTG ATAAGGTGCC GTGCTTGGCT 
TCGATGATAG AAGATGTTCT AGGGGAAGGG TCCGTCTCGG CCAGCCGCTT TAGTAGGTGG 
TTTTCTAACC CCAGCCGATC AGGAAGCCGG TCCAGCAGTC TTGGGTCAAC ACCACATGAA 
GAACTGGAGA GACTTGCAGG TCTGGAGCAG GTCATCCTCT CTCCTGCACA GAACTCGGGG 
AATTACTTTG CTCCTATACC ATCAGAAGAT CATGCTGAAA ATAAAGTGGA TATTTTAGAA 
ATGCTACAGA AAGCCAAAGT GGACTTAAAA CCTCTTCTCT CCAGCCTTTC TGCAAATAAA 
GAAAAGCTTA AAGAAAGCTC ACATTCAGGG GTTGTGCTCT CAGTGGAAGA GGTAGAAGCA 
GGGCTCAAGG GCTTGAAGGT GGACCAGCAC ATGAAGAATT CAACTCCTTT CATGGCAGAA 
CATTTAGAGG AGACCCTGAG TGCTGTAACA AGCAACCGGC AGCTCAGAAA GGACGGCGAT 
ATGACTGCAT TCAACAAGCT AGTGAGCAGC ATGAAAGCAA GCGGGACTCT GCCCTCGCAG 
CCCAAAGCCA GTCGAAACCT TGAAAGCCAT TTGATGTCCC CTGCTGAGCT TCCAGGCCAG 
GCTGTCCCTA AGAACATCCT CCAGGAACTT CTGGGTCCAC CAGTTCAGAG ACCGGCTTCT 
TCTAATCTTC TGAGTGGCCT GATGGGGAGC TTGGAGCCTA CAGCATCTTT ACTGGGCCAA 
AGGGCACCCT CTCCTCCCTT GTCACAGGCA TTTCAAACTC GAGCAGCCTC GGCAGACTAC 
CTTCGCCCTA GAATATCTTC ACCAGTGGGT TTCCCACCAG GACCACCTCC GCAGCTTCTT 
GGGGACCCGT TCCAAGGCAT GCGCAAGCCC ATGAGCCCCA TCACAGTCCA GATGAGCCAG 
TTGGAGTTGC AACAGGCCGC TCTGGAGGGG CTGGCCTTGC CACATGACCT TGCAATGCAG 
ACAGCAAACT TCTACCAGCC TGGTTTTGGC AAACCACAAG TGGACAGAAC CAGAGATGGA 
TACAGAAACA GGCAACAGCG AGTGACCAAG TCGCCAGCAA ACATGCATCA AGGGAATTCC 
TCTTCCCCTG CTCCCTCGGC CTCCGTCACA AGCATGCTTT CTCCCTCCTT CACCCCTACC 
TCGGTGATTC GAAAGATGTT CGAGAGCAAA GAGAAAAGCA AGGAGGAGCC TGCCTCTGGG 
AAAGCCGCTC TTGGTGACCA TAAAGAGGGC ACTCAGGAGG CCAGTGAAGA CAACCTGCTG 
TCATCCAACC CCATGTCCAG TGCTGATCGA GACTCTTCTC CCACGACAAA TCCCAAGATG 
TCAACGTTGC AGCGGTCTTC CTGCTCTACC CCTCTGTCCC AAACCAACCG TTACACCAAA 
GAACAAGATT ATCGACCTAA AGCAGCCGGG AGGAAAACAC CCACCTTGGC CTCCCCAGTT 
CCAGGAGCAC CTTTTCTCCG CCCTGTCCAC CAAGTCCCCC TGGTCCCCCA TGTACCAATC 
GTTCGACCTG CTCACCAGCT TCACCCAGGC CTGGTCCAAA GGATGCATAT TCAACAGCAT 
CTTCCAAGTT TGCTCCAAGC TGGTATGCTT CCACCAGGGA TGGACCTGAC GCATTTACAG 
GGTATATCTG GCCCAATCCT GGGTCAGCCT TTTTACCCTT TACCCGCTGC TAATCACCCT 
CTACTAAACC CTCGTCCCGG GACACCTCTG CATCTGGCAA TGATGCAGCA GCAGTTACAG 
CGCTCAGTTC TGCATCCTCC AGGCTCCGGG TCTCAAGCAG CTGCTGTTAG CGTTCCCGTA 
ACCCCCCAGA ATGTGCCCAG CAGGTCGGGC CTGCCCCACG TGCACCCCCA GCTGGAGCAC 
CGCCCTAGCC AGAGGAGCAG CTCTCCGGTG GGCCTTGCCA AGTGGTTTGG CTCAGATGTG 
CTGCAGCAAC CCTTGCCTTC TATGCCTGCC AAAGTCATCA GTGTAGACGA GCTGGAGTAC 
CGACCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4ENIF1 ( XM_004716706.1 ) cDNA ORF clone, Echinops telfairi(small Madagascar hedgehog) -> XP_004716763.1 Echinops telfairi eukaryotic translation initiation factor 4E nuclear import factor 1 (EIF4ENIF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OEc23831
Clone ID Related Accession (Same CDS sequence)	XM_004716706.1
Accession Version	XM_004716706.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-18
Organism	Echinops telfairi(small Madagascar hedgehog)
Product	eukaryotic translation initiation factor 4E transporter isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022103926.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Echinops telfairi Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAGGA GAAGCGCGGG TGAAGCTGAG AATGGAGACG CTTTCCTTGA CCTGAAGAAG 
CTGCCAACCT CCAAATCCCC CCATCGCTAC ACAAAAGAGG AGCTTTTGGA TATAAAAGAG 
CGGCCCCATT CCAAACAGAG ACCTCCATGC CTCTCTGAAA AATATGACAG TGATGGCGTC 
TGGGACCCTG AGAAGTGGCA TGCCTCTCTC TACCCAGTTT CAGGGCGGAG CTCACCTGTG 
GAAAACCAGA AAAAAGAGCT GGATACAGAC CGGCCTTCCC TAGTGCGTCG GATAGTAGAT 
CCACGGGAGC GTGTGAAGGA AGACGATTTG GATGTTGTTC TCAGTCCACA GAGACGAAGC 
TTTGGTGGTG GCTGCCATGT GACGGCTGCT GTAAGCTCCC GACGCTCGGG GAGCCCACTG 
GAGAAAGACA GTGATGGGCT TCGTCTGCTT GGCGGACGGA GGATCGGCAG TGGGAGAATA 
ATGTCTGCCC GGACCTTTGA AAAGGACCAC CGTCTTAGTG ATAAGGACCT TCGGGACTTG 
AGAGACAGAG ACCGTGAAAG GGACTATAAG GACAAGCGTT TCAGGAGAGA GTTTGGGGAT 
AGTAAGCGTG TCTTTGGCGA GCGTAGAAGA AATGATTCTT ACACGGAAGA AGAACCAGAG 
TGGTTCTCAG CTGGACCCAC GAGCCAGTCT GAAACCATTG AGCTGACTGG CTTTGATGAC 
AAGATCCTAG AAGAAGACCA CAAAGGGAGA AAGAGAACGA GGCGGCGGAC AGCTTCTGTG 
AAGGAAGGCA TAGTCGAGTG CAATGGAGGA GTGGCCGAAG AGGAGGAGGT GGAGGTCATT 
CTTGCACAGG AGCCTGCTGC TGATCAGGAA GTACCGAGGG ACGCCGTCTT GCCTGAGCAG 
TCCCCAGGAG AATTTGACTT TAATGAGTTC TTTAACCTTG ATAAGGTGCC GTGCTTGGCT 
TCGATGATAG AAGATGTTCT AGGGGAAGGG TCCGTCTCGG CCAGCCGCTT TAGTAGGTGG 
TTTTCTAACC CCAGCCGATC AGGAAGCCGG TCCAGCAGTC TTGGGTCAAC ACCACATGAA 
GAACTGGAGA GACTTGCAGG TCTGGAGCAG GTCATCCTCT CTCCTGCACA GAACTCGGGG 
AATTACTTTG CTCCTATACC ATCAGAAGAT CATGCTGAAA ATAAAGTGGA TATTTTAGAA 
ATGCTACAGA AAGCCAAAGT GGACTTAAAA CCTCTTCTCT CCAGCCTTTC TGCAAATAAA 
GAAAAGCTTA AAGAAAGCTC ACATTCAGGG GTTGTGCTCT CAGTGGAAGA GGTAGAAGCA 
GGGCTCAAGG GCTTGAAGGT GGACCAGCAC ATGAAGAATT CAACTCCTTT CATGGCAGAA 
CATTTAGAGG AGACCCTGAG TGCTGTAACA AGCAACCGGC AGCTCAGAAA GGACGGCGAT 
ATGACTGCAT TCAACAAGCT AGTGAGCAGC ATGAAAGCAA GCGGGACTCT GCCCTCGCAG 
CCCAAAGCCA GTGAACTTCT GGGTCCACCA GTTCAGAGAC CGGCTTCTTC TAATCTTCTG 
AGTGGCCTGA TGGGGAGCTT GGAGCCTACA GCATCTTTAC TGGGCCAAAG GGCACCCTCT 
CCTCCCTTGT CACAGGCATT TCAAACTCGA GCAGCCTCGG CAGACTACCT TCGCCCTAGA 
ATATCTTCAC CAGTGGGTTT CCCACCAGGA CCACCTCCGC AGCTTCTTGG GGACCCGTTC 
CAAGGCATGC GCAAGCCCAT GAGCCCCATC ACAGTCCAGA TGAGCCAGTT GGAGTTGCAA 
CAGGCCGCTC TGGAGGGGCT GGCCTTGCCA CATGACCTTG CAATGCAGAC AGCAAACTTC 
TACCAGCCTG GTTTTGGCAA ACCACAAGTG GACAGAACCA GAGATGGATA CAGAAACAGG 
CAACAGCGAG TGACCAAGTC GCCAGCAAAC ATGCATCAAG GGAATTCCTC TTCCCCTGCT 
CCCTCGGCCT CCGTCACAAG CATGCTTTCT CCCTCCTTCA CCCCTACCTC GGTGATTCGA 
AAGATGTTCG AGAGCAAAGA GAAAAGCAAG GAGGAGCCTG CCTCTGGGAA AGCCGCTCTT 
GGTGACCATA AAGAGGGCAC TCAGGAGGCC AGTGAAGACA ACCTGCTGTC ATCCAACCCC 
ATGTCCAGTG CTGATCGAGA CTCTTCTCCC ACGACAAATC CCAAGATGTC AACGTTGCAG 
CGGTCTTCCT GCTCTACCCC TCTGTCCCAA ACCAACCGTT ACACCAAAGA ACAAGATTAT 
CGACCTAAAG CAGCCGGGAG GAAAACACCC ACCTTGGCCT CCCCAGTTCC AGGAGCACCT 
TTTCTCCGCC CTGTCCACCA AGTCCCCCTG GTCCCCCATG TACCAATCGT TCGACCTGCT 
CACCAGCTTC ACCCAGGCCT GGTCCAAAGG ATGCATATTC AACAGCATCT TCCAAGTTTG 
CTCCAAGCTG GTATGCTTCC ACCAGGGATG GACCTGACGC ATTTACAGGG TATATCTGGC 
CCAATCCTGG GTCAGCCTTT TTACCCTTTA CCCGCTGCTA ATCACCCTCT ACTAAACCCT 
CGTCCCGGGA CACCTCTGCA TCTGGCAATG ATGCAGCAGC AGTTACAGCG CTCAGTTCTG 
CATCCTCCAG GCTCCGGGTC TCAAGCAGCT GCTGTTAGCG TTCCCGTAAC CCCCCAGAAT 
GTGCCCAGCA GGTCGGGCCT GCCCCACGTG CACCCCCAGC TGGAGCACCG CCCTAGCCAG 
AGGAGCAGCT CTCCGGTGGG CCTTGCCAAG TGGTTTGGCT CAGATGTGCT GCAGCAACCC 
TTGCCTTCTA TGCCTGCCAA AGTCATCAGT GTAGACGAGC TGGAGTACCG ACCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004716763.1
CDS	22..2898
Translation	MDRRSAGEAENGDAFLDLKKLPTSKSPHRYTKEELLDIKERPHSKQRPPCLSEKYDSDGVWDPEKWHASLYPVSGRSSPVENQKKELDTDRPSLVRRIVDPRERVKEDDLDVVLSPQRRSFGGGCHVTAAVSSRRSGSPLEKDSDGLRLLGGRRIGSGRIMSARTFEKDHRLSDKDLRDLRDRDRERDYKDKRFRREFGDSKRVFGERRRNDSYTEEEPEWFSAGPTSQSETIELTGFDDKILEEDHKGRKRTRRRTASVKEGIVECNGGVAEEEEVEVILAQEPAADQEVPRDAVLPEQSPGEFDFNEFFNLDKVPCLASMIEDVLGEGSVSASRFSRWFSNPSRSGSRSSSLGSTPHEELERLAGLEQVILSPAQNSGNYFAPIPSEDHAENKVDILEMLQKAKVDLKPLLSSLSANKEKLKESSHSGVVLSVEEVEAGLKGLKVDQHMKNSTPFMAEHLEETLSAVTSNRQLRKDGDMTAFNKLVSSMKASGTLPSQPKASELLGPPVQRPASSNLLSGLMGSLEPTASLLGQRAPSPPLSQAFQTRAASADYLRPRISSPVGFPPGPPPQLLGDPFQGMRKPMSPITVQMSQLELQQAALEGLALPHDLAMQTANFYQPGFGKPQVDRTRDGYRNRQQRVTKSPANMHQGNSSSPAPSASVTSMLSPSFTPTSVIRKMFESKEKSKEEPASGKAALGDHKEGTQEASEDNLLSSNPMSSADRDSSPTTNPKMSTLQRSSCSTPLSQTNRYTKEQDYRPKAAGRKTPTLASPVPGAPFLRPVHQVPLVPHVPIVRPAHQLHPGLVQRMHIQQHLPSLLQAGMLPPGMDLTHLQGISGPILGQPFYPLPAANHPLLNPRPGTPLHLAMMQQQLQRSVLHPPGSGSQAAAVSVPVTPQNVPSRSGLPHVHPQLEHRPSQRSSSPVGLAKWFGSDVLQQPLPSMPAKVISVDELEYRP

Target ORF information:

RefSeq Version	XM_004716706.1
Organism	Echinops telfairi(small Madagascar hedgehog)
Definition	Echinops telfairi eukaryotic translation initiation factor 4E nuclear import factor 1 (EIF4ENIF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004716706.1

ORF Insert Sequence:

ATGGATAGGA GAAGCGCGGG TGAAGCTGAG AATGGAGACG CTTTCCTTGA CCTGAAGAAG 
CTGCCAACCT CCAAATCCCC CCATCGCTAC ACAAAAGAGG AGCTTTTGGA TATAAAAGAG 
CGGCCCCATT CCAAACAGAG ACCTCCATGC CTCTCTGAAA AATATGACAG TGATGGCGTC 
TGGGACCCTG AGAAGTGGCA TGCCTCTCTC TACCCAGTTT CAGGGCGGAG CTCACCTGTG 
GAAAACCAGA AAAAAGAGCT GGATACAGAC CGGCCTTCCC TAGTGCGTCG GATAGTAGAT 
CCACGGGAGC GTGTGAAGGA AGACGATTTG GATGTTGTTC TCAGTCCACA GAGACGAAGC 
TTTGGTGGTG GCTGCCATGT GACGGCTGCT GTAAGCTCCC GACGCTCGGG GAGCCCACTG 
GAGAAAGACA GTGATGGGCT TCGTCTGCTT GGCGGACGGA GGATCGGCAG TGGGAGAATA 
ATGTCTGCCC GGACCTTTGA AAAGGACCAC CGTCTTAGTG ATAAGGACCT TCGGGACTTG 
AGAGACAGAG ACCGTGAAAG GGACTATAAG GACAAGCGTT TCAGGAGAGA GTTTGGGGAT 
AGTAAGCGTG TCTTTGGCGA GCGTAGAAGA AATGATTCTT ACACGGAAGA AGAACCAGAG 
TGGTTCTCAG CTGGACCCAC GAGCCAGTCT GAAACCATTG AGCTGACTGG CTTTGATGAC 
AAGATCCTAG AAGAAGACCA CAAAGGGAGA AAGAGAACGA GGCGGCGGAC AGCTTCTGTG 
AAGGAAGGCA TAGTCGAGTG CAATGGAGGA GTGGCCGAAG AGGAGGAGGT GGAGGTCATT 
CTTGCACAGG AGCCTGCTGC TGATCAGGAA GTACCGAGGG ACGCCGTCTT GCCTGAGCAG 
TCCCCAGGAG AATTTGACTT TAATGAGTTC TTTAACCTTG ATAAGGTGCC GTGCTTGGCT 
TCGATGATAG AAGATGTTCT AGGGGAAGGG TCCGTCTCGG CCAGCCGCTT TAGTAGGTGG 
TTTTCTAACC CCAGCCGATC AGGAAGCCGG TCCAGCAGTC TTGGGTCAAC ACCACATGAA 
GAACTGGAGA GACTTGCAGG TCTGGAGCAG GTCATCCTCT CTCCTGCACA GAACTCGGGG 
AATTACTTTG CTCCTATACC ATCAGAAGAT CATGCTGAAA ATAAAGTGGA TATTTTAGAA 
ATGCTACAGA AAGCCAAAGT GGACTTAAAA CCTCTTCTCT CCAGCCTTTC TGCAAATAAA 
GAAAAGCTTA AAGAAAGCTC ACATTCAGGG GTTGTGCTCT CAGTGGAAGA GGTAGAAGCA 
GGGCTCAAGG GCTTGAAGGT GGACCAGCAC ATGAAGAATT CAACTCCTTT CATGGCAGAA 
CATTTAGAGG AGACCCTGAG TGCTGTAACA AGCAACCGGC AGCTCAGAAA GGACGGCGAT 
ATGACTGCAT TCAACAAGCT AGTGAGCAGC ATGAAAGCAA GCGGGACTCT GCCCTCGCAG 
CCCAAAGCCA GTGAACTTCT GGGTCCACCA GTTCAGAGAC CGGCTTCTTC TAATCTTCTG 
AGTGGCCTGA TGGGGAGCTT GGAGCCTACA GCATCTTTAC TGGGCCAAAG GGCACCCTCT 
CCTCCCTTGT CACAGGCATT TCAAACTCGA GCAGCCTCGG CAGACTACCT TCGCCCTAGA 
ATATCTTCAC CAGTGGGTTT CCCACCAGGA CCACCTCCGC AGCTTCTTGG GGACCCGTTC 
CAAGGCATGC GCAAGCCCAT GAGCCCCATC ACAGTCCAGA TGAGCCAGTT GGAGTTGCAA 
CAGGCCGCTC TGGAGGGGCT GGCCTTGCCA CATGACCTTG CAATGCAGAC AGCAAACTTC 
TACCAGCCTG GTTTTGGCAA ACCACAAGTG GACAGAACCA GAGATGGATA CAGAAACAGG 
CAACAGCGAG TGACCAAGTC GCCAGCAAAC ATGCATCAAG GGAATTCCTC TTCCCCTGCT 
CCCTCGGCCT CCGTCACAAG CATGCTTTCT CCCTCCTTCA CCCCTACCTC GGTGATTCGA 
AAGATGTTCG AGAGCAAAGA GAAAAGCAAG GAGGAGCCTG CCTCTGGGAA AGCCGCTCTT 
GGTGACCATA AAGAGGGCAC TCAGGAGGCC AGTGAAGACA ACCTGCTGTC ATCCAACCCC 
ATGTCCAGTG CTGATCGAGA CTCTTCTCCC ACGACAAATC CCAAGATGTC AACGTTGCAG 
CGGTCTTCCT GCTCTACCCC TCTGTCCCAA ACCAACCGTT ACACCAAAGA ACAAGATTAT 
CGACCTAAAG CAGCCGGGAG GAAAACACCC ACCTTGGCCT CCCCAGTTCC AGGAGCACCT 
TTTCTCCGCC CTGTCCACCA AGTCCCCCTG GTCCCCCATG TACCAATCGT TCGACCTGCT 
CACCAGCTTC ACCCAGGCCT GGTCCAAAGG ATGCATATTC AACAGCATCT TCCAAGTTTG 
CTCCAAGCTG GTATGCTTCC ACCAGGGATG GACCTGACGC ATTTACAGGG TATATCTGGC 
CCAATCCTGG GTCAGCCTTT TTACCCTTTA CCCGCTGCTA ATCACCCTCT ACTAAACCCT 
CGTCCCGGGA CACCTCTGCA TCTGGCAATG ATGCAGCAGC AGTTACAGCG CTCAGTTCTG 
CATCCTCCAG GCTCCGGGTC TCAAGCAGCT GCTGTTAGCG TTCCCGTAAC CCCCCAGAAT 
GTGCCCAGCA GGTCGGGCCT GCCCCACGTG CACCCCCAGC TGGAGCACCG CCCTAGCCAG 
AGGAGCAGCT CTCCGGTGGG CCTTGCCAAG TGGTTTGGCT CAGATGTGCT GCAGCAACCC 
TTGCCTTCTA TGCCTGCCAA AGTCATCAGT GTAGACGAGC TGGAGTACCG ACCGTGA