ThrRS cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ThrRS
Entrez Gene ID	45784
Full Name	Threonyl-tRNA synthetase
Synonyms	42/3,Aats-thr,CG5353,Dmel\CG5353,P539,TRS,l(2)k04203,threonyl-tRNA synthetase
General protein information	Preferred Names Threonyl-tRNA synthetase Names CG5353 gene product from transcript CG5353-RD CG5353-PA CG5353-PB CG5353-PC CG5353-PD ThrRS-PA ThrRS-PB ThrRS-PC ThrRS-PD threonyl-tRNA synthetase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 33C4-33C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_080100.6	NP_524839.3	Threonyl-tRNA synthetase, isoform B
NM_164994.3	NP_723725.1	Threonyl-tRNA synthetase, isoform A
NM_001298939.1	NP_001285868.1	Threonyl-tRNA synthetase, isoform D
NM_164995.2	NP_723726.1	Threonyl-tRNA synthetase, isoform C

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	tars	NP_001116258.1
Drosophila melanogaster (fruit fly)	Aats-thr	NP_524839.3
Caenorhabditis elegans (roundworm)	tars-1	NP_001022033.1
Magnaporthe oryzae (rice blast fungus)	MGG_03551	XP_003716364.1
Arabidopsis thaliana (thale cress)	AT5G26830	NP_198035.2
Rattus norvegicus (Norway rat)	Tars	NP_001006977.1
Gallus gallus (chicken)	TARS	NP_001026618.1
Saccharomyces cerevisiae (baker's yeast)	THS1	NP_116578.3
Schizosaccharomyces pombe (fission yeast)	trs1	NP_596364.1
Canis lupus familiaris (dog)	TARS	XP_536509.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008929	XP_319682.4
Eremothecium gossypii	AGOS_ACL039W	NP_983365.1
Neurospora crassa	NCU02380	XP_959801.1
Homo sapiens (human)	TARS	NP_001245367.1
Oryza sativa (rice)	Os08g0295300	NP_001061475.1
Xenopus tropicalis (tropical clawed frog)	tars	NP_001001219.1
Pan troglodytes (chimpanzee)	TARS	XP_003310808.1
Bos taurus (cattle)	TARS	NP_001029542.1
Mus musculus (house mouse)	Tars	NP_149065.2
Kluyveromyces lactis	KLLA0F05918g	XP_455348.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ThrRS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ThrRS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15227	NM_164995.2 Latest version!	Drosophila melanogaster Threonyl-tRNA synthetase (ThrRS), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa15227	NM_080100.6 Latest version!	Drosophila melanogaster Threonyl-tRNA synthetase (ThrRS), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa15228	NM_001298939.1 Latest version!	Drosophila melanogaster Threonyl-tRNA synthetase (ThrRS), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa15228	NM_164994.3 Latest version!	Drosophila melanogaster Threonyl-tRNA synthetase (ThrRS), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ThrRS ( NM_080100.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524839.3 Drosophila melanogaster Threonyl-tRNA synthetase (ThrRS), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa15227
Clone ID Related Accession (Same CDS sequence)	NM_080100.6 , NM_164995.2
Accession Version	NM_080100.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Threonyl-tRNA synthetase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_080100.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGAAGG AGAAAAAGGA GAAGCCATCC GGCGGTGGAG ATACTCGCAA GGAACTCAGT 
CCCTTGCCCA AGTACATCGA GGAGCGCAAT GTCTTCTGGG AGAAGTGCAA GGCGGAATAC 
GAGGCCGAAC TGGCGGCCAA GAAGCGTGAG CCCATCAAGG TGACACTGCC CGATGGCAAG 
CAGGTGGACG CCACCTCCTG GGAGACCACT CCGTACGAAG TGGCCCGCGG CATTAGTCAG 
GGTCTCGCTG ACAACACCGT CATCTCCAAG GTGAACGGCG AGGTGTGGGA TTTGGACCGT 
GTGCTCGAGG GAAACTGCAC CCTGCAGCTT CTCAAGTTCG ACGACCCTGA GGCGCAGGCC 
GTCTTCTGGC ACAGCTCTGC CCACATCATG GGTGAGGCCA TGGAGCGCAT CTATGGTGGA 
CACCTGTGCT ACGGTCCGCC CATTGAAAAC GGCTTCTACT ACGACATGCA TCTGGAGGGC 
GAGGGTATCT CCACCAACGA CTACGGCGCC ATGGAGGGTC TGGTTAAGCA AATTGTCAAG 
GAGAAGCAGA ACTTCGAACG TCTGGAGATG AAGAAGTCCG ACCTGCTGGA GATGTTCAAA 
TACAATGAGT TCAAGGTGCG CATTCTCAAC GAGAAGGTGA CCACCGACCG CACCACTGTC 
TACAAGTGCG GTTCTTTGAT CGATCTCTGC CGCGGACCTC ATGTTCGTCA CACCGGCAAG 
GTGAAGGCCC TGAAGATCAC CAAGAACTCC TCTACCTACT GGGAGGGTAA GGCTGACGCT 
GAGACACTGC AACGCGTCTA CGGCATCTCG TTCCCCGACC CGAAGCAGCT CAAGGAGTGG 
GAGAAACTTC AGGAGGAGGC GGCTAAGCGT GATCACCGCA AAATCGGTCG CGAGCAGGAG 
CTGTTCTTCT TCCATGAACT TTCGCCTGGC TCATGCTTTT TCCAGCCGCG AGGAGCGCAC 
ATCTACAACA CCCTGATGGG CTTCATCAAG GCGGAATATA GAAAGCGTGG TTTCCAAGAA 
GTCATTTCCC CCAACATCTA CAACGCTAAG TTGTGGATGA CCTCCGGCCA CTGGCAGCAC 
TATGCCGAGA ACATGTTCTC CTTCGAGGCC GAGAAGGAGA AGTTCGCCCT CAAGCCCATG 
AATTGTCCAG GACATTGTCT CATCTTCGAT AACCGCAATC GATCCTGGCG AGAGCTGCCA 
CTGCGAATGG CCGACTTCGG TGTGCTTCAT CGCAACGAGC TCTCCGGTGC TTTAACCGGA 
TTAACTCGCG TGCGTCGCTT CCAGCAGGAC GATGCGCACA TCTTCTGTGC CCCCGAGCAG 
ATTAAAAGCG AAATGAAGGG CTGCCTGGAG TTCCTGAAGT ATGTGTATAC CATTTTCGGA 
TTCTCCTTCC AGCTGGTGCT GTCCACACGC CCGGATAATT ACCTTGGCGA GTTGGAACAG 
TGGAATGATG CGGAAAAGGC GCTGGCTGAA TCACTCAACG AGTTTGGAAT GCCGTGGAAG 
GAGAACCCCG GCGATGGCGC CTTCTACGGA CCGAAGATTG ATATTACCAT TATGGATGCA 
CTGAAGCGTG CCCATCAGTG CGCCACCATC CAGTTGGACT TCCAGCTGCC AATTCGTTTT 
AATCTCAGCT ACATCGCCGA CGATGGCGAG AAGAAGAGGC CGGTGATCAT CCATCGCGCT 
ATTCTTGGCT CAGTGGAGCG GATGATTGCC ATTCTCACGG AGAACTTTGC TGGCAAGTGG 
CCCTTCTGGC TATCACCGCG CCAGGTGATG GTCGTTCCAG TGGGTCCTGC CTATGATCAG 
TACGCCCAGT CAGTGCGGGA TCAGTTGCAC GATGCTGGAT TCATGAGCGA GGCGGATTGC 
GATGCCGGCG ACACGATGAA CAAGAAGATA CGCAATGCTC AGTTGGCCCA GTTCAACTTC 
ATTCTGGTGG TGGGCGACAA GGAGCGCTCC TCGAATACCG TTAACGTGCG CACTCGGGAC 
AACAAGGTGC ACGGCGAGGT CTCCGTGGCG GAACTCATCA CAAAGCTGCA AAAGATTCGC 
GACGAATTCA TCGCCAACGA GGACAGCTTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524839.3
CDS	94..2166
Translation	MKKEKKEKPSGGGDTRKELSPLPKYIEERNVFWEKCKAEYEAELAAKKREPIKVTLPDGKQVDATSWETTPYEVARGISQGLADNTVISKVNGEVWDLDRVLEGNCTLQLLKFDDPEAQAVFWHSSAHIMGEAMERIYGGHLCYGPPIENGFYYDMHLEGEGISTNDYGAMEGLVKQIVKEKQNFERLEMKKSDLLEMFKYNEFKVRILNEKVTTDRTTVYKCGSLIDLCRGPHVRHTGKVKALKITKNSSTYWEGKADAETLQRVYGISFPDPKQLKEWEKLQEEAAKRDHRKIGREQELFFFHELSPGSCFFQPRGAHIYNTLMGFIKAEYRKRGFQEVISPNIYNAKLWMTSGHWQHYAENMFSFEAEKEKFALKPMNCPGHCLIFDNRNRSWRELPLRMADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAPEQIKSEMKGCLEFLKYVYTIFGFSFQLVLSTRPDNYLGELEQWNDAEKALAESLNEFGMPWKENPGDGAFYGPKIDITIMDALKRAHQCATIQLDFQLPIRFNLSYIADDGEKKRPVIIHRAILGSVERMIAILTENFAGKWPFWLSPRQVMVVPVGPAYDQYAQSVRDQLHDAGFMSEADCDAGDTMNKKIRNAQLAQFNFILVVGDKERSSNTVNVRTRDNKVHGEVSVAELITKLQKIRDEFIANEDSF

Target ORF information:

RefSeq Version	NM_080100.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Threonyl-tRNA synthetase (ThrRS), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080100.6

ORF Insert Sequence:

ATGAAGAAGG AGAAAAAGGA GAAGCCATCC GGCGGTGGAG ATACTCGCAA GGAACTCAGT 
CCCTTGCCCA AGTACATCGA GGAGCGCAAT GTCTTCTGGG AGAAGTGCAA GGCGGAATAC 
GAGGCCGAAC TGGCGGCCAA GAAGCGTGAG CCCATCAAGG TGACACTGCC CGATGGCAAG 
CAGGTGGACG CCACCTCCTG GGAGACCACT CCGTACGAAG TGGCCCGCGG CATTAGTCAG 
GGTCTCGCTG ACAACACCGT CATCTCCAAG GTGAACGGCG AGGTGTGGGA TTTGGACCGT 
GTGCTCGAGG GAAACTGCAC CCTGCAGCTT CTCAAGTTCG ACGACCCTGA GGCGCAGGCC 
GTCTTCTGGC ACAGCTCTGC CCACATCATG GGTGAGGCCA TGGAGCGCAT CTATGGTGGA 
CACCTGTGCT ACGGTCCGCC CATTGAAAAC GGCTTCTACT ACGACATGCA TCTGGAGGGC 
GAGGGTATCT CCACCAACGA CTACGGCGCC ATGGAGGGTC TGGTTAAGCA AATTGTCAAG 
GAGAAGCAGA ACTTCGAACG TCTGGAGATG AAGAAGTCCG ACCTGCTGGA GATGTTCAAA 
TACAATGAGT TCAAGGTGCG CATTCTCAAC GAGAAGGTGA CCACCGACCG CACCACTGTC 
TACAAGTGCG GTTCTTTGAT CGATCTCTGC CGCGGACCTC ATGTTCGTCA CACCGGCAAG 
GTGAAGGCCC TGAAGATCAC CAAGAACTCC TCTACCTACT GGGAGGGTAA GGCTGACGCT 
GAGACACTGC AACGCGTCTA CGGCATCTCG TTCCCCGACC CGAAGCAGCT CAAGGAGTGG 
GAGAAACTTC AGGAGGAGGC GGCTAAGCGT GATCACCGCA AAATCGGTCG CGAGCAGGAG 
CTGTTCTTCT TCCATGAACT TTCGCCTGGC TCATGCTTTT TCCAGCCGCG AGGAGCGCAC 
ATCTACAACA CCCTGATGGG CTTCATCAAG GCGGAATATA GAAAGCGTGG TTTCCAAGAA 
GTCATTTCCC CCAACATCTA CAACGCTAAG TTGTGGATGA CCTCCGGCCA CTGGCAGCAC 
TATGCCGAGA ACATGTTCTC CTTCGAGGCC GAGAAGGAGA AGTTCGCCCT CAAGCCCATG 
AATTGTCCAG GACATTGTCT CATCTTCGAT AACCGCAATC GATCCTGGCG AGAGCTGCCA 
CTGCGAATGG CCGACTTCGG TGTGCTTCAT CGCAACGAGC TCTCCGGTGC TTTAACCGGA 
TTAACTCGCG TGCGTCGCTT CCAGCAGGAC GATGCGCACA TCTTCTGTGC CCCCGAGCAG 
ATTAAAAGCG AAATGAAGGG CTGCCTGGAG TTCCTGAAGT ATGTGTATAC CATTTTCGGA 
TTCTCCTTCC AGCTGGTGCT GTCCACACGC CCGGATAATT ACCTTGGCGA GTTGGAACAG 
TGGAATGATG CGGAAAAGGC GCTGGCTGAA TCACTCAACG AGTTTGGAAT GCCGTGGAAG 
GAGAACCCCG GCGATGGCGC CTTCTACGGA CCGAAGATTG ATATTACCAT TATGGATGCA 
CTGAAGCGTG CCCATCAGTG CGCCACCATC CAGTTGGACT TCCAGCTGCC AATTCGTTTT 
AATCTCAGCT ACATCGCCGA CGATGGCGAG AAGAAGAGGC CGGTGATCAT CCATCGCGCT 
ATTCTTGGCT CAGTGGAGCG GATGATTGCC ATTCTCACGG AGAACTTTGC TGGCAAGTGG 
CCCTTCTGGC TATCACCGCG CCAGGTGATG GTCGTTCCAG TGGGTCCTGC CTATGATCAG 
TACGCCCAGT CAGTGCGGGA TCAGTTGCAC GATGCTGGAT TCATGAGCGA GGCGGATTGC 
GATGCCGGCG ACACGATGAA CAAGAAGATA CGCAATGCTC AGTTGGCCCA GTTCAACTTC 
ATTCTGGTGG TGGGCGACAA GGAGCGCTCC TCGAATACCG TTAACGTGCG CACTCGGGAC 
AACAAGGTGC ACGGCGAGGT CTCCGTGGCG GAACTCATCA CAAAGCTGCA AAAGATTCGC 
GACGAATTCA TCGCCAACGA GGACAGCTTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ThrRS ( NM_164995.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723726.1 Drosophila melanogaster Threonyl-tRNA synthetase (ThrRS), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa15227
Clone ID Related Accession (Same CDS sequence)	NM_080100.6 , NM_164995.2
Accession Version	NM_164995.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Threonyl-tRNA synthetase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Dec 17, 2009 this sequence version replaced NM_164995.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGAAGG AGAAAAAGGA GAAGCCATCC GGCGGTGGAG ATACTCGCAA GGAACTCAGT 
CCCTTGCCCA AGTACATCGA GGAGCGCAAT GTCTTCTGGG AGAAGTGCAA GGCGGAATAC 
GAGGCCGAAC TGGCGGCCAA GAAGCGTGAG CCCATCAAGG TGACACTGCC CGATGGCAAG 
CAGGTGGACG CCACCTCCTG GGAGACCACT CCGTACGAAG TGGCCCGCGG CATTAGTCAG 
GGTCTCGCTG ACAACACCGT CATCTCCAAG GTGAACGGCG AGGTGTGGGA TTTGGACCGT 
GTGCTCGAGG GAAACTGCAC CCTGCAGCTT CTCAAGTTCG ACGACCCTGA GGCGCAGGCC 
GTCTTCTGGC ACAGCTCTGC CCACATCATG GGTGAGGCCA TGGAGCGCAT CTATGGTGGA 
CACCTGTGCT ACGGTCCGCC CATTGAAAAC GGCTTCTACT ACGACATGCA TCTGGAGGGC 
GAGGGTATCT CCACCAACGA CTACGGCGCC ATGGAGGGTC TGGTTAAGCA AATTGTCAAG 
GAGAAGCAGA ACTTCGAACG TCTGGAGATG AAGAAGTCCG ACCTGCTGGA GATGTTCAAA 
TACAATGAGT TCAAGGTGCG CATTCTCAAC GAGAAGGTGA CCACCGACCG CACCACTGTC 
TACAAGTGCG GTTCTTTGAT CGATCTCTGC CGCGGACCTC ATGTTCGTCA CACCGGCAAG 
GTGAAGGCCC TGAAGATCAC CAAGAACTCC TCTACCTACT GGGAGGGTAA GGCTGACGCT 
GAGACACTGC AACGCGTCTA CGGCATCTCG TTCCCCGACC CGAAGCAGCT CAAGGAGTGG 
GAGAAACTTC AGGAGGAGGC GGCTAAGCGT GATCACCGCA AAATCGGTCG CGAGCAGGAG 
CTGTTCTTCT TCCATGAACT TTCGCCTGGC TCATGCTTTT TCCAGCCGCG AGGAGCGCAC 
ATCTACAACA CCCTGATGGG CTTCATCAAG GCGGAATATA GAAAGCGTGG TTTCCAAGAA 
GTCATTTCCC CCAACATCTA CAACGCTAAG TTGTGGATGA CCTCCGGCCA CTGGCAGCAC 
TATGCCGAGA ACATGTTCTC CTTCGAGGCC GAGAAGGAGA AGTTCGCCCT CAAGCCCATG 
AATTGTCCAG GACATTGTCT CATCTTCGAT AACCGCAATC GATCCTGGCG AGAGCTGCCA 
CTGCGAATGG CCGACTTCGG TGTGCTTCAT CGCAACGAGC TCTCCGGTGC TTTAACCGGA 
TTAACTCGCG TGCGTCGCTT CCAGCAGGAC GATGCGCACA TCTTCTGTGC CCCCGAGCAG 
ATTAAAAGCG AAATGAAGGG CTGCCTGGAG TTCCTGAAGT ATGTGTATAC CATTTTCGGA 
TTCTCCTTCC AGCTGGTGCT GTCCACACGC CCGGATAATT ACCTTGGCGA GTTGGAACAG 
TGGAATGATG CGGAAAAGGC GCTGGCTGAA TCACTCAACG AGTTTGGAAT GCCGTGGAAG 
GAGAACCCCG GCGATGGCGC CTTCTACGGA CCGAAGATTG ATATTACCAT TATGGATGCA 
CTGAAGCGTG CCCATCAGTG CGCCACCATC CAGTTGGACT TCCAGCTGCC AATTCGTTTT 
AATCTCAGCT ACATCGCCGA CGATGGCGAG AAGAAGAGGC CGGTGATCAT CCATCGCGCT 
ATTCTTGGCT CAGTGGAGCG GATGATTGCC ATTCTCACGG AGAACTTTGC TGGCAAGTGG 
CCCTTCTGGC TATCACCGCG CCAGGTGATG GTCGTTCCAG TGGGTCCTGC CTATGATCAG 
TACGCCCAGT CAGTGCGGGA TCAGTTGCAC GATGCTGGAT TCATGAGCGA GGCGGATTGC 
GATGCCGGCG ACACGATGAA CAAGAAGATA CGCAATGCTC AGTTGGCCCA GTTCAACTTC 
ATTCTGGTGG TGGGCGACAA GGAGCGCTCC TCGAATACCG TTAACGTGCG CACTCGGGAC 
AACAAGGTGC ACGGCGAGGT CTCCGTGGCG GAACTCATCA CAAAGCTGCA AAAGATTCGC 
GACGAATTCA TCGCCAACGA GGACAGCTTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723726.1
CDS	110..2182
Translation	MKKEKKEKPSGGGDTRKELSPLPKYIEERNVFWEKCKAEYEAELAAKKREPIKVTLPDGKQVDATSWETTPYEVARGISQGLADNTVISKVNGEVWDLDRVLEGNCTLQLLKFDDPEAQAVFWHSSAHIMGEAMERIYGGHLCYGPPIENGFYYDMHLEGEGISTNDYGAMEGLVKQIVKEKQNFERLEMKKSDLLEMFKYNEFKVRILNEKVTTDRTTVYKCGSLIDLCRGPHVRHTGKVKALKITKNSSTYWEGKADAETLQRVYGISFPDPKQLKEWEKLQEEAAKRDHRKIGREQELFFFHELSPGSCFFQPRGAHIYNTLMGFIKAEYRKRGFQEVISPNIYNAKLWMTSGHWQHYAENMFSFEAEKEKFALKPMNCPGHCLIFDNRNRSWRELPLRMADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAPEQIKSEMKGCLEFLKYVYTIFGFSFQLVLSTRPDNYLGELEQWNDAEKALAESLNEFGMPWKENPGDGAFYGPKIDITIMDALKRAHQCATIQLDFQLPIRFNLSYIADDGEKKRPVIIHRAILGSVERMIAILTENFAGKWPFWLSPRQVMVVPVGPAYDQYAQSVRDQLHDAGFMSEADCDAGDTMNKKIRNAQLAQFNFILVVGDKERSSNTVNVRTRDNKVHGEVSVAELITKLQKIRDEFIANEDSF

Target ORF information:

RefSeq Version	NM_164995.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Threonyl-tRNA synthetase (ThrRS), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164995.2

ORF Insert Sequence:

ATGAAGAAGG AGAAAAAGGA GAAGCCATCC GGCGGTGGAG ATACTCGCAA GGAACTCAGT 
CCCTTGCCCA AGTACATCGA GGAGCGCAAT GTCTTCTGGG AGAAGTGCAA GGCGGAATAC 
GAGGCCGAAC TGGCGGCCAA GAAGCGTGAG CCCATCAAGG TGACACTGCC CGATGGCAAG 
CAGGTGGACG CCACCTCCTG GGAGACCACT CCGTACGAAG TGGCCCGCGG CATTAGTCAG 
GGTCTCGCTG ACAACACCGT CATCTCCAAG GTGAACGGCG AGGTGTGGGA TTTGGACCGT 
GTGCTCGAGG GAAACTGCAC CCTGCAGCTT CTCAAGTTCG ACGACCCTGA GGCGCAGGCC 
GTCTTCTGGC ACAGCTCTGC CCACATCATG GGTGAGGCCA TGGAGCGCAT CTATGGTGGA 
CACCTGTGCT ACGGTCCGCC CATTGAAAAC GGCTTCTACT ACGACATGCA TCTGGAGGGC 
GAGGGTATCT CCACCAACGA CTACGGCGCC ATGGAGGGTC TGGTTAAGCA AATTGTCAAG 
GAGAAGCAGA ACTTCGAACG TCTGGAGATG AAGAAGTCCG ACCTGCTGGA GATGTTCAAA 
TACAATGAGT TCAAGGTGCG CATTCTCAAC GAGAAGGTGA CCACCGACCG CACCACTGTC 
TACAAGTGCG GTTCTTTGAT CGATCTCTGC CGCGGACCTC ATGTTCGTCA CACCGGCAAG 
GTGAAGGCCC TGAAGATCAC CAAGAACTCC TCTACCTACT GGGAGGGTAA GGCTGACGCT 
GAGACACTGC AACGCGTCTA CGGCATCTCG TTCCCCGACC CGAAGCAGCT CAAGGAGTGG 
GAGAAACTTC AGGAGGAGGC GGCTAAGCGT GATCACCGCA AAATCGGTCG CGAGCAGGAG 
CTGTTCTTCT TCCATGAACT TTCGCCTGGC TCATGCTTTT TCCAGCCGCG AGGAGCGCAC 
ATCTACAACA CCCTGATGGG CTTCATCAAG GCGGAATATA GAAAGCGTGG TTTCCAAGAA 
GTCATTTCCC CCAACATCTA CAACGCTAAG TTGTGGATGA CCTCCGGCCA CTGGCAGCAC 
TATGCCGAGA ACATGTTCTC CTTCGAGGCC GAGAAGGAGA AGTTCGCCCT CAAGCCCATG 
AATTGTCCAG GACATTGTCT CATCTTCGAT AACCGCAATC GATCCTGGCG AGAGCTGCCA 
CTGCGAATGG CCGACTTCGG TGTGCTTCAT CGCAACGAGC TCTCCGGTGC TTTAACCGGA 
TTAACTCGCG TGCGTCGCTT CCAGCAGGAC GATGCGCACA TCTTCTGTGC CCCCGAGCAG 
ATTAAAAGCG AAATGAAGGG CTGCCTGGAG TTCCTGAAGT ATGTGTATAC CATTTTCGGA 
TTCTCCTTCC AGCTGGTGCT GTCCACACGC CCGGATAATT ACCTTGGCGA GTTGGAACAG 
TGGAATGATG CGGAAAAGGC GCTGGCTGAA TCACTCAACG AGTTTGGAAT GCCGTGGAAG 
GAGAACCCCG GCGATGGCGC CTTCTACGGA CCGAAGATTG ATATTACCAT TATGGATGCA 
CTGAAGCGTG CCCATCAGTG CGCCACCATC CAGTTGGACT TCCAGCTGCC AATTCGTTTT 
AATCTCAGCT ACATCGCCGA CGATGGCGAG AAGAAGAGGC CGGTGATCAT CCATCGCGCT 
ATTCTTGGCT CAGTGGAGCG GATGATTGCC ATTCTCACGG AGAACTTTGC TGGCAAGTGG 
CCCTTCTGGC TATCACCGCG CCAGGTGATG GTCGTTCCAG TGGGTCCTGC CTATGATCAG 
TACGCCCAGT CAGTGCGGGA TCAGTTGCAC GATGCTGGAT TCATGAGCGA GGCGGATTGC 
GATGCCGGCG ACACGATGAA CAAGAAGATA CGCAATGCTC AGTTGGCCCA GTTCAACTTC 
ATTCTGGTGG TGGGCGACAA GGAGCGCTCC TCGAATACCG TTAACGTGCG CACTCGGGAC 
AACAAGGTGC ACGGCGAGGT CTCCGTGGCG GAACTCATCA CAAAGCTGCA AAAGATTCGC 
GACGAATTCA TCGCCAACGA GGACAGCTTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ThrRS ( NM_164994.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723725.1 Drosophila melanogaster Threonyl-tRNA synthetase (ThrRS), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa15228
Clone ID Related Accession (Same CDS sequence)	NM_164994.3 , NM_001298939.1
Accession Version	NM_164994.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Threonyl-tRNA synthetase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_164994.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGAAGT TGGCGCCGCA GGGCGGCAGA TATTATAGCC AAATAAGCCG CATTTGCCAG 
TGGATTTTGC CGAAAATTCA GACGCAGTCG AAGCCACCAA AACAACAACA ACAACAGCAA 
CCACAATTGA AAGAACAACA ACAACACAGC TACGCAACTT TGGCTGCCAA GATGAAGAAG 
GAGAAAAAGG AGAAGCCATC CGGCGGTGGA GATACTCGCA AGGAACTCAG TCCCTTGCCC 
AAGTACATCG AGGAGCGCAA TGTCTTCTGG GAGAAGTGCA AGGCGGAATA CGAGGCCGAA 
CTGGCGGCCA AGAAGCGTGA GCCCATCAAG GTGACACTGC CCGATGGCAA GCAGGTGGAC 
GCCACCTCCT GGGAGACCAC TCCGTACGAA GTGGCCCGCG GCATTAGTCA GGGTCTCGCT 
GACAACACCG TCATCTCCAA GGTGAACGGC GAGGTGTGGG ATTTGGACCG TGTGCTCGAG 
GGAAACTGCA CCCTGCAGCT TCTCAAGTTC GACGACCCTG AGGCGCAGGC CGTCTTCTGG 
CACAGCTCTG CCCACATCAT GGGTGAGGCC ATGGAGCGCA TCTATGGTGG ACACCTGTGC 
TACGGTCCGC CCATTGAAAA CGGCTTCTAC TACGACATGC ATCTGGAGGG CGAGGGTATC 
TCCACCAACG ACTACGGCGC CATGGAGGGT CTGGTTAAGC AAATTGTCAA GGAGAAGCAG 
AACTTCGAAC GTCTGGAGAT GAAGAAGTCC GACCTGCTGG AGATGTTCAA ATACAATGAG 
TTCAAGGTGC GCATTCTCAA CGAGAAGGTG ACCACCGACC GCACCACTGT CTACAAGTGC 
GGTTCTTTGA TCGATCTCTG CCGCGGACCT CATGTTCGTC ACACCGGCAA GGTGAAGGCC 
CTGAAGATCA CCAAGAACTC CTCTACCTAC TGGGAGGGTA AGGCTGACGC TGAGACACTG 
CAACGCGTCT ACGGCATCTC GTTCCCCGAC CCGAAGCAGC TCAAGGAGTG GGAGAAACTT 
CAGGAGGAGG CGGCTAAGCG TGATCACCGC AAAATCGGTC GCGAGCAGGA GCTGTTCTTC 
TTCCATGAAC TTTCGCCTGG CTCATGCTTT TTCCAGCCGC GAGGAGCGCA CATCTACAAC 
ACCCTGATGG GCTTCATCAA GGCGGAATAT AGAAAGCGTG GTTTCCAAGA AGTCATTTCC 
CCCAACATCT ACAACGCTAA GTTGTGGATG ACCTCCGGCC ACTGGCAGCA CTATGCCGAG 
AACATGTTCT CCTTCGAGGC CGAGAAGGAG AAGTTCGCCC TCAAGCCCAT GAATTGTCCA 
GGACATTGTC TCATCTTCGA TAACCGCAAT CGATCCTGGC GAGAGCTGCC ACTGCGAATG 
GCCGACTTCG GTGTGCTTCA TCGCAACGAG CTCTCCGGTG CTTTAACCGG ATTAACTCGC 
GTGCGTCGCT TCCAGCAGGA CGATGCGCAC ATCTTCTGTG CCCCCGAGCA GATTAAAAGC 
GAAATGAAGG GCTGCCTGGA GTTCCTGAAG TATGTGTATA CCATTTTCGG ATTCTCCTTC 
CAGCTGGTGC TGTCCACACG CCCGGATAAT TACCTTGGCG AGTTGGAACA GTGGAATGAT 
GCGGAAAAGG CGCTGGCTGA ATCACTCAAC GAGTTTGGAA TGCCGTGGAA GGAGAACCCC 
GGCGATGGCG CCTTCTACGG ACCGAAGATT GATATTACCA TTATGGATGC ACTGAAGCGT 
GCCCATCAGT GCGCCACCAT CCAGTTGGAC TTCCAGCTGC CAATTCGTTT TAATCTCAGC 
TACATCGCCG ACGATGGCGA GAAGAAGAGG CCGGTGATCA TCCATCGCGC TATTCTTGGC 
TCAGTGGAGC GGATGATTGC CATTCTCACG GAGAACTTTG CTGGCAAGTG GCCCTTCTGG 
CTATCACCGC GCCAGGTGAT GGTCGTTCCA GTGGGTCCTG CCTATGATCA GTACGCCCAG 
TCAGTGCGGG ATCAGTTGCA CGATGCTGGA TTCATGAGCG AGGCGGATTG CGATGCCGGC 
GACACGATGA ACAAGAAGAT ACGCAATGCT CAGTTGGCCC AGTTCAACTT CATTCTGGTG 
GTGGGCGACA AGGAGCGCTC CTCGAATACC GTTAACGTGC GCACTCGGGA CAACAAGGTG 
CACGGCGAGG TCTCCGTGGC GGAACTCATC ACAAAGCTGC AAAAGATTCG CGACGAATTC 
ATCGCCAACG AGGACAGCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723725.1
CDS	145..2388
Translation	MLKLAPQGGRYYSQISRICQWILPKIQTQSKPPKQQQQQQPQLKEQQQHSYATLAAKMKKEKKEKPSGGGDTRKELSPLPKYIEERNVFWEKCKAEYEAELAAKKREPIKVTLPDGKQVDATSWETTPYEVARGISQGLADNTVISKVNGEVWDLDRVLEGNCTLQLLKFDDPEAQAVFWHSSAHIMGEAMERIYGGHLCYGPPIENGFYYDMHLEGEGISTNDYGAMEGLVKQIVKEKQNFERLEMKKSDLLEMFKYNEFKVRILNEKVTTDRTTVYKCGSLIDLCRGPHVRHTGKVKALKITKNSSTYWEGKADAETLQRVYGISFPDPKQLKEWEKLQEEAAKRDHRKIGREQELFFFHELSPGSCFFQPRGAHIYNTLMGFIKAEYRKRGFQEVISPNIYNAKLWMTSGHWQHYAENMFSFEAEKEKFALKPMNCPGHCLIFDNRNRSWRELPLRMADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAPEQIKSEMKGCLEFLKYVYTIFGFSFQLVLSTRPDNYLGELEQWNDAEKALAESLNEFGMPWKENPGDGAFYGPKIDITIMDALKRAHQCATIQLDFQLPIRFNLSYIADDGEKKRPVIIHRAILGSVERMIAILTENFAGKWPFWLSPRQVMVVPVGPAYDQYAQSVRDQLHDAGFMSEADCDAGDTMNKKIRNAQLAQFNFILVVGDKERSSNTVNVRTRDNKVHGEVSVAELITKLQKIRDEFIANEDSF

Target ORF information:

RefSeq Version	NM_164994.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Threonyl-tRNA synthetase (ThrRS), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164994.3

ORF Insert Sequence:

ATGTTGAAGT TGGCGCCGCA GGGCGGCAGA TATTATAGCC AAATAAGCCG CATTTGCCAG 
TGGATTTTGC CGAAAATTCA GACGCAGTCG AAGCCACCAA AACAACAACA ACAACAGCAA 
CCACAATTGA AAGAACAACA ACAACACAGC TACGCAACTT TGGCTGCCAA GATGAAGAAG 
GAGAAAAAGG AGAAGCCATC CGGCGGTGGA GATACTCGCA AGGAACTCAG TCCCTTGCCC 
AAGTACATCG AGGAGCGCAA TGTCTTCTGG GAGAAGTGCA AGGCGGAATA CGAGGCCGAA 
CTGGCGGCCA AGAAGCGTGA GCCCATCAAG GTGACACTGC CCGATGGCAA GCAGGTGGAC 
GCCACCTCCT GGGAGACCAC TCCGTACGAA GTGGCCCGCG GCATTAGTCA GGGTCTCGCT 
GACAACACCG TCATCTCCAA GGTGAACGGC GAGGTGTGGG ATTTGGACCG TGTGCTCGAG 
GGAAACTGCA CCCTGCAGCT TCTCAAGTTC GACGACCCTG AGGCGCAGGC CGTCTTCTGG 
CACAGCTCTG CCCACATCAT GGGTGAGGCC ATGGAGCGCA TCTATGGTGG ACACCTGTGC 
TACGGTCCGC CCATTGAAAA CGGCTTCTAC TACGACATGC ATCTGGAGGG CGAGGGTATC 
TCCACCAACG ACTACGGCGC CATGGAGGGT CTGGTTAAGC AAATTGTCAA GGAGAAGCAG 
AACTTCGAAC GTCTGGAGAT GAAGAAGTCC GACCTGCTGG AGATGTTCAA ATACAATGAG 
TTCAAGGTGC GCATTCTCAA CGAGAAGGTG ACCACCGACC GCACCACTGT CTACAAGTGC 
GGTTCTTTGA TCGATCTCTG CCGCGGACCT CATGTTCGTC ACACCGGCAA GGTGAAGGCC 
CTGAAGATCA CCAAGAACTC CTCTACCTAC TGGGAGGGTA AGGCTGACGC TGAGACACTG 
CAACGCGTCT ACGGCATCTC GTTCCCCGAC CCGAAGCAGC TCAAGGAGTG GGAGAAACTT 
CAGGAGGAGG CGGCTAAGCG TGATCACCGC AAAATCGGTC GCGAGCAGGA GCTGTTCTTC 
TTCCATGAAC TTTCGCCTGG CTCATGCTTT TTCCAGCCGC GAGGAGCGCA CATCTACAAC 
ACCCTGATGG GCTTCATCAA GGCGGAATAT AGAAAGCGTG GTTTCCAAGA AGTCATTTCC 
CCCAACATCT ACAACGCTAA GTTGTGGATG ACCTCCGGCC ACTGGCAGCA CTATGCCGAG 
AACATGTTCT CCTTCGAGGC CGAGAAGGAG AAGTTCGCCC TCAAGCCCAT GAATTGTCCA 
GGACATTGTC TCATCTTCGA TAACCGCAAT CGATCCTGGC GAGAGCTGCC ACTGCGAATG 
GCCGACTTCG GTGTGCTTCA TCGCAACGAG CTCTCCGGTG CTTTAACCGG ATTAACTCGC 
GTGCGTCGCT TCCAGCAGGA CGATGCGCAC ATCTTCTGTG CCCCCGAGCA GATTAAAAGC 
GAAATGAAGG GCTGCCTGGA GTTCCTGAAG TATGTGTATA CCATTTTCGG ATTCTCCTTC 
CAGCTGGTGC TGTCCACACG CCCGGATAAT TACCTTGGCG AGTTGGAACA GTGGAATGAT 
GCGGAAAAGG CGCTGGCTGA ATCACTCAAC GAGTTTGGAA TGCCGTGGAA GGAGAACCCC 
GGCGATGGCG CCTTCTACGG ACCGAAGATT GATATTACCA TTATGGATGC ACTGAAGCGT 
GCCCATCAGT GCGCCACCAT CCAGTTGGAC TTCCAGCTGC CAATTCGTTT TAATCTCAGC 
TACATCGCCG ACGATGGCGA GAAGAAGAGG CCGGTGATCA TCCATCGCGC TATTCTTGGC 
TCAGTGGAGC GGATGATTGC CATTCTCACG GAGAACTTTG CTGGCAAGTG GCCCTTCTGG 
CTATCACCGC GCCAGGTGAT GGTCGTTCCA GTGGGTCCTG CCTATGATCA GTACGCCCAG 
TCAGTGCGGG ATCAGTTGCA CGATGCTGGA TTCATGAGCG AGGCGGATTG CGATGCCGGC 
GACACGATGA ACAAGAAGAT ACGCAATGCT CAGTTGGCCC AGTTCAACTT CATTCTGGTG 
GTGGGCGACA AGGAGCGCTC CTCGAATACC GTTAACGTGC GCACTCGGGA CAACAAGGTG 
CACGGCGAGG TCTCCGTGGC GGAACTCATC ACAAAGCTGC AAAAGATTCG CGACGAATTC 
ATCGCCAACG AGGACAGCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ThrRS ( NM_001298939.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285868.1 Drosophila melanogaster Threonyl-tRNA synthetase (ThrRS), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa15228
Clone ID Related Accession (Same CDS sequence)	NM_164994.3 , NM_001298939.1
Accession Version	NM_001298939.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Threonyl-tRNA synthetase, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGAAGT TGGCGCCGCA GGGCGGCAGA TATTATAGCC AAATAAGCCG CATTTGCCAG 
TGGATTTTGC CGAAAATTCA GACGCAGTCG AAGCCACCAA AACAACAACA ACAACAGCAA 
CCACAATTGA AAGAACAACA ACAACACAGC TACGCAACTT TGGCTGCCAA GATGAAGAAG 
GAGAAAAAGG AGAAGCCATC CGGCGGTGGA GATACTCGCA AGGAACTCAG TCCCTTGCCC 
AAGTACATCG AGGAGCGCAA TGTCTTCTGG GAGAAGTGCA AGGCGGAATA CGAGGCCGAA 
CTGGCGGCCA AGAAGCGTGA GCCCATCAAG GTGACACTGC CCGATGGCAA GCAGGTGGAC 
GCCACCTCCT GGGAGACCAC TCCGTACGAA GTGGCCCGCG GCATTAGTCA GGGTCTCGCT 
GACAACACCG TCATCTCCAA GGTGAACGGC GAGGTGTGGG ATTTGGACCG TGTGCTCGAG 
GGAAACTGCA CCCTGCAGCT TCTCAAGTTC GACGACCCTG AGGCGCAGGC CGTCTTCTGG 
CACAGCTCTG CCCACATCAT GGGTGAGGCC ATGGAGCGCA TCTATGGTGG ACACCTGTGC 
TACGGTCCGC CCATTGAAAA CGGCTTCTAC TACGACATGC ATCTGGAGGG CGAGGGTATC 
TCCACCAACG ACTACGGCGC CATGGAGGGT CTGGTTAAGC AAATTGTCAA GGAGAAGCAG 
AACTTCGAAC GTCTGGAGAT GAAGAAGTCC GACCTGCTGG AGATGTTCAA ATACAATGAG 
TTCAAGGTGC GCATTCTCAA CGAGAAGGTG ACCACCGACC GCACCACTGT CTACAAGTGC 
GGTTCTTTGA TCGATCTCTG CCGCGGACCT CATGTTCGTC ACACCGGCAA GGTGAAGGCC 
CTGAAGATCA CCAAGAACTC CTCTACCTAC TGGGAGGGTA AGGCTGACGC TGAGACACTG 
CAACGCGTCT ACGGCATCTC GTTCCCCGAC CCGAAGCAGC TCAAGGAGTG GGAGAAACTT 
CAGGAGGAGG CGGCTAAGCG TGATCACCGC AAAATCGGTC GCGAGCAGGA GCTGTTCTTC 
TTCCATGAAC TTTCGCCTGG CTCATGCTTT TTCCAGCCGC GAGGAGCGCA CATCTACAAC 
ACCCTGATGG GCTTCATCAA GGCGGAATAT AGAAAGCGTG GTTTCCAAGA AGTCATTTCC 
CCCAACATCT ACAACGCTAA GTTGTGGATG ACCTCCGGCC ACTGGCAGCA CTATGCCGAG 
AACATGTTCT CCTTCGAGGC CGAGAAGGAG AAGTTCGCCC TCAAGCCCAT GAATTGTCCA 
GGACATTGTC TCATCTTCGA TAACCGCAAT CGATCCTGGC GAGAGCTGCC ACTGCGAATG 
GCCGACTTCG GTGTGCTTCA TCGCAACGAG CTCTCCGGTG CTTTAACCGG ATTAACTCGC 
GTGCGTCGCT TCCAGCAGGA CGATGCGCAC ATCTTCTGTG CCCCCGAGCA GATTAAAAGC 
GAAATGAAGG GCTGCCTGGA GTTCCTGAAG TATGTGTATA CCATTTTCGG ATTCTCCTTC 
CAGCTGGTGC TGTCCACACG CCCGGATAAT TACCTTGGCG AGTTGGAACA GTGGAATGAT 
GCGGAAAAGG CGCTGGCTGA ATCACTCAAC GAGTTTGGAA TGCCGTGGAA GGAGAACCCC 
GGCGATGGCG CCTTCTACGG ACCGAAGATT GATATTACCA TTATGGATGC ACTGAAGCGT 
GCCCATCAGT GCGCCACCAT CCAGTTGGAC TTCCAGCTGC CAATTCGTTT TAATCTCAGC 
TACATCGCCG ACGATGGCGA GAAGAAGAGG CCGGTGATCA TCCATCGCGC TATTCTTGGC 
TCAGTGGAGC GGATGATTGC CATTCTCACG GAGAACTTTG CTGGCAAGTG GCCCTTCTGG 
CTATCACCGC GCCAGGTGAT GGTCGTTCCA GTGGGTCCTG CCTATGATCA GTACGCCCAG 
TCAGTGCGGG ATCAGTTGCA CGATGCTGGA TTCATGAGCG AGGCGGATTG CGATGCCGGC 
GACACGATGA ACAAGAAGAT ACGCAATGCT CAGTTGGCCC AGTTCAACTT CATTCTGGTG 
GTGGGCGACA AGGAGCGCTC CTCGAATACC GTTAACGTGC GCACTCGGGA CAACAAGGTG 
CACGGCGAGG TCTCCGTGGC GGAACTCATC ACAAAGCTGC AAAAGATTCG CGACGAATTC 
ATCGCCAACG AGGACAGCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285868.1
CDS	145..2388
Translation	MLKLAPQGGRYYSQISRICQWILPKIQTQSKPPKQQQQQQPQLKEQQQHSYATLAAKMKKEKKEKPSGGGDTRKELSPLPKYIEERNVFWEKCKAEYEAELAAKKREPIKVTLPDGKQVDATSWETTPYEVARGISQGLADNTVISKVNGEVWDLDRVLEGNCTLQLLKFDDPEAQAVFWHSSAHIMGEAMERIYGGHLCYGPPIENGFYYDMHLEGEGISTNDYGAMEGLVKQIVKEKQNFERLEMKKSDLLEMFKYNEFKVRILNEKVTTDRTTVYKCGSLIDLCRGPHVRHTGKVKALKITKNSSTYWEGKADAETLQRVYGISFPDPKQLKEWEKLQEEAAKRDHRKIGREQELFFFHELSPGSCFFQPRGAHIYNTLMGFIKAEYRKRGFQEVISPNIYNAKLWMTSGHWQHYAENMFSFEAEKEKFALKPMNCPGHCLIFDNRNRSWRELPLRMADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAPEQIKSEMKGCLEFLKYVYTIFGFSFQLVLSTRPDNYLGELEQWNDAEKALAESLNEFGMPWKENPGDGAFYGPKIDITIMDALKRAHQCATIQLDFQLPIRFNLSYIADDGEKKRPVIIHRAILGSVERMIAILTENFAGKWPFWLSPRQVMVVPVGPAYDQYAQSVRDQLHDAGFMSEADCDAGDTMNKKIRNAQLAQFNFILVVGDKERSSNTVNVRTRDNKVHGEVSVAELITKLQKIRDEFIANEDSF

Target ORF information:

RefSeq Version	NM_001298939.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Threonyl-tRNA synthetase (ThrRS), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298939.1

ORF Insert Sequence:

ATGTTGAAGT TGGCGCCGCA GGGCGGCAGA TATTATAGCC AAATAAGCCG CATTTGCCAG 
TGGATTTTGC CGAAAATTCA GACGCAGTCG AAGCCACCAA AACAACAACA ACAACAGCAA 
CCACAATTGA AAGAACAACA ACAACACAGC TACGCAACTT TGGCTGCCAA GATGAAGAAG 
GAGAAAAAGG AGAAGCCATC CGGCGGTGGA GATACTCGCA AGGAACTCAG TCCCTTGCCC 
AAGTACATCG AGGAGCGCAA TGTCTTCTGG GAGAAGTGCA AGGCGGAATA CGAGGCCGAA 
CTGGCGGCCA AGAAGCGTGA GCCCATCAAG GTGACACTGC CCGATGGCAA GCAGGTGGAC 
GCCACCTCCT GGGAGACCAC TCCGTACGAA GTGGCCCGCG GCATTAGTCA GGGTCTCGCT 
GACAACACCG TCATCTCCAA GGTGAACGGC GAGGTGTGGG ATTTGGACCG TGTGCTCGAG 
GGAAACTGCA CCCTGCAGCT TCTCAAGTTC GACGACCCTG AGGCGCAGGC CGTCTTCTGG 
CACAGCTCTG CCCACATCAT GGGTGAGGCC ATGGAGCGCA TCTATGGTGG ACACCTGTGC 
TACGGTCCGC CCATTGAAAA CGGCTTCTAC TACGACATGC ATCTGGAGGG CGAGGGTATC 
TCCACCAACG ACTACGGCGC CATGGAGGGT CTGGTTAAGC AAATTGTCAA GGAGAAGCAG 
AACTTCGAAC GTCTGGAGAT GAAGAAGTCC GACCTGCTGG AGATGTTCAA ATACAATGAG 
TTCAAGGTGC GCATTCTCAA CGAGAAGGTG ACCACCGACC GCACCACTGT CTACAAGTGC 
GGTTCTTTGA TCGATCTCTG CCGCGGACCT CATGTTCGTC ACACCGGCAA GGTGAAGGCC 
CTGAAGATCA CCAAGAACTC CTCTACCTAC TGGGAGGGTA AGGCTGACGC TGAGACACTG 
CAACGCGTCT ACGGCATCTC GTTCCCCGAC CCGAAGCAGC TCAAGGAGTG GGAGAAACTT 
CAGGAGGAGG CGGCTAAGCG TGATCACCGC AAAATCGGTC GCGAGCAGGA GCTGTTCTTC 
TTCCATGAAC TTTCGCCTGG CTCATGCTTT TTCCAGCCGC GAGGAGCGCA CATCTACAAC 
ACCCTGATGG GCTTCATCAA GGCGGAATAT AGAAAGCGTG GTTTCCAAGA AGTCATTTCC 
CCCAACATCT ACAACGCTAA GTTGTGGATG ACCTCCGGCC ACTGGCAGCA CTATGCCGAG 
AACATGTTCT CCTTCGAGGC CGAGAAGGAG AAGTTCGCCC TCAAGCCCAT GAATTGTCCA 
GGACATTGTC TCATCTTCGA TAACCGCAAT CGATCCTGGC GAGAGCTGCC ACTGCGAATG 
GCCGACTTCG GTGTGCTTCA TCGCAACGAG CTCTCCGGTG CTTTAACCGG ATTAACTCGC 
GTGCGTCGCT TCCAGCAGGA CGATGCGCAC ATCTTCTGTG CCCCCGAGCA GATTAAAAGC 
GAAATGAAGG GCTGCCTGGA GTTCCTGAAG TATGTGTATA CCATTTTCGG ATTCTCCTTC 
CAGCTGGTGC TGTCCACACG CCCGGATAAT TACCTTGGCG AGTTGGAACA GTGGAATGAT 
GCGGAAAAGG CGCTGGCTGA ATCACTCAAC GAGTTTGGAA TGCCGTGGAA GGAGAACCCC 
GGCGATGGCG CCTTCTACGG ACCGAAGATT GATATTACCA TTATGGATGC ACTGAAGCGT 
GCCCATCAGT GCGCCACCAT CCAGTTGGAC TTCCAGCTGC CAATTCGTTT TAATCTCAGC 
TACATCGCCG ACGATGGCGA GAAGAAGAGG CCGGTGATCA TCCATCGCGC TATTCTTGGC 
TCAGTGGAGC GGATGATTGC CATTCTCACG GAGAACTTTG CTGGCAAGTG GCCCTTCTGG 
CTATCACCGC GCCAGGTGAT GGTCGTTCCA GTGGGTCCTG CCTATGATCA GTACGCCCAG 
TCAGTGCGGG ATCAGTTGCA CGATGCTGGA TTCATGAGCG AGGCGGATTG CGATGCCGGC 
GACACGATGA ACAAGAAGAT ACGCAATGCT CAGTTGGCCC AGTTCAACTT CATTCTGGTG 
GTGGGCGACA AGGAGCGCTC CTCGAATACC GTTAACGTGC GCACTCGGGA CAACAAGGTG 
CACGGCGAGG TCTCCGTGGC GGAACTCATC ACAAAGCTGC AAAAGATTCG CGACGAATTC 
ATCGCCAACG AGGACAGCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ThrRS cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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