ey cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ey
Entrez Gene ID	43812
Full Name	eyeless
Synonyms	CG1464,DPax-6,Dmel\CG1464,EY,EYEL,Ey,Ey/Pax6,OK107,Pax-6,Pax6,eye,l(4)102CDh,l(4)102CDr,l(4)33,pax6
General protein information	Preferred Names eyeless Names CG1464 gene product from transcript CG1464-RB CG1464-PA CG1464-PB CG1464-PC CG1464-PD ey-PA ey-PB ey-PC ey-PD tumor-head-63
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 4 Map Location: 102D4-102D5\|4-2.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_079889.3	NP_524628.2	eyeless, isoform A
NM_001014694.2	NP_001014694.1	eyeless, isoform C
NM_166789.2	NP_726607.1	eyeless, isoform B
NM_001014693.2	NP_001014693.1	eyeless, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Eyeless (Ey), Drosophila homolog of Pax6, regulates the production of glial cells in the brain.
Title: eyeless/Pax6 controls the production of glial cells in the visual center of Drosophila melanogaster.

Reducing levels of old intermediate neural progenitor (INP) temporal transcription factor Eyeless/Pax6 allows Notch signaling to promote dedifferentiation of INP progeny into ectopic INPs, creating a proliferative mass of ectopic progenitors in the brain.
Title: Aging Neural Progenitors Lose Competence to Respond to Mitogenic Notch Signaling.

The third helix of the Ey HD can directly interact with the RED subdomain in Ey Paired domain.
Title: The homeodomain of Eyeless regulates cell growth and antagonizes the paired domain-dependent retinal differentiation function.

Daughterless homodimer synergizes with Eyeless to induce Atonal expression and retinal neuron differentiation.
Title: Daughterless homodimer synergizes with Eyeless to induce Atonal expression and retinal neuron differentiation.

Data indicate that Hipk as a repressor of both Twin of eyeless (Toy) and Eyeless (Ey).
Title: Hipk promotes photoreceptor differentiation through the repression of Twin of eyeless and Eyeless expression.

The following ey gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ey cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00291	NM_166789.2 Latest version!	Drosophila melanogaster eyeless (ey), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa00292	NM_001014693.2 Latest version!	Drosophila melanogaster eyeless (ey), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa00293	NM_079889.3 Latest version!	Drosophila melanogaster eyeless (ey), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa00294	NM_001014694.2 Latest version!	Drosophila melanogaster eyeless (ey), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ey ( NM_166789.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726607.1 Drosophila melanogaster eyeless (ey), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa00291
Clone ID Related Accession (Same CDS sequence)	NM_166789.2
Accession Version	NM_166789.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eyeless, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_166789.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGACAG CCACTCCTCT TAACTCTTCG GAAAGCGGTG GCGCAAGCAA CTCCGGGGAG 
GGTAGTGAAC AGGAGGCGAT TTACGAGAAG CTTCGGCTGT TAAATACTCA GCACGCTGCA 
GGACCAGGAC CACTGGAGCC TGCCAGAGCA GCGCCCTTGG TAGGTCAATC ACCCAACCAC 
CTAGGAACCC GATCCAGCCA CCCCCAGCTG GTGCACGGTA ACCATCAGGC ACTACAGCAG 
CATCAACAGC AGAGCTGGCC GCCCCGTCAC TATTCCGGAT CTTGGTACCC CACCTCTCTT 
AGCGAAATAC CCATCTCATC GGCTCCCAAT ATCGCATCCG TTACGGCGTA TGCATCAGGA 
CCTTCACTTG CTCACTCACT GAGTCCACCC AACGACATCG AAAGCCTGGC CAGTATCGGT 
CACCAGAGAA ACTGCCCCGT TGCAACGGAG GACATACATT TAAAAAAAGA ACTTGATGGT 
CATCAGTCCG ATGAAACGGG CTCCGGTGAA GGTGAAAACT CCAATGGTGG CGCTTCAAAT 
ATAGGAAACA CTGAGGATGA TCAAGCTCGG CTCATACTAA AAAGAAAGTT GCAACGCAAT 
CGAACATCTT TCACGAACGA CCAGATAGAC AGTCTTGAAA AAGAGTTTGA ACGAACACAC 
TATCCAGATG TTTTTGCCCG CGAACGTTTG GCTGGAAAGA TTGGGTTGCC AGAGGCAAGA 
ATTCAGGTTT GGTTCTCAAA CCGTCGAGCA AAATGGCGTC GCGAGGAGAA GCTGCGAAAC 
CAGCGAAGAA CACCAAATTC CACAGGAGCT AGTGCAACTT CTTCCTCTAC ATCGGCAACC 
GCCTCTTTGA CTGACAGCCC TAACAGCCTA AGTGCTTGTT CCTCGCTGCT GTCCGGATCA 
GCTGGGGGTC CCTCAGTCAG TACCATTAAT GGCTTATCGT CTCCAAGCAC ATTGTCTACT 
AATGTCAATG CTCCAACGCT TGGCGCTGGG ATCGATAGCT CTGAAAGCCC AACACCAATC 
CCGCACATTC GGCCTAGCTG CACCTCTGAC AATGACAATG GTCGTCAAAG TGAAGATTGC 
AGAAGAGTTT GTTCTCCATG CCCACTTGGC GTTGGCGGGC ATCAAAATAC TCATCATATC 
CAGAGCAATG GTCACGCCCA AGGTCATGCA CTTGTTCCTG CCATTTCGCC ACGACTCAAT 
TTTAATAGTG GTAGTTTCGG CGCGATGTAC TCCAACATGC ATCATACGGC GTTATCCATG 
AGCGATTCAT ATGGGGCGGT TACGCCGATT CCGAGCTTTA ACCACTCAGC TGTCGGTCCG 
CTGGCTCCGC CATCGCCAAT ACCGCAACAG GGCGATCTTA CCCCTTCCTC GTTATATCCG 
TGCCACATGA CCCTACGACC CCCTCCGATG GCTCCCGCTC ACCATCACAT CGTGCCGGGT 
GACGGTGGCA GACCTGCGGG CGTTGGCCTA GGCAGTGGCC AATCTGCGAA TTTGGGAGCA 
AGCTGCAGCG GATCGGGATA CGAAGTGCTA TCTGCCTACG CGTTGCCACC GCCCCCTATG 
GCGTCGAGCT CTGCTGCTGA TTCAAGCTTC TCAGCCGCGT CCAGTGCCAG CGCTAATGTG 
ACCCCACATC ACACCATAGC CCAAGAATCA TGCCCCTCTC CGTGTTCAAG CGCGAGCCAC 
TTTGGAGTTG CTCACAGTTC TGGGTTTTCG TCCGACCCGA TTTCACCGGC TGTATCTTCG 
TATGCACATA TGAGCTACAA TTACGCGTCG TCCGCTAACA CCATGACGCC TTCCTCCGCC 
AGCGGCACAT CAGCACACGT GGCCCCGGGA AAACAACAGT TCTTCGCCTC CTGTTTCTAC 
TCACCGTGGG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726607.1
CDS	729..2603
Translation	MQTATPLNSSESGGASNSGEGSEQEAIYEKLRLLNTQHAAGPGPLEPARAAPLVGQSPNHLGTRSSHPQLVHGNHQALQQHQQQSWPPRHYSGSWYPTSLSEIPISSAPNIASVTAYASGPSLAHSLSPPNDIESLASIGHQRNCPVATEDIHLKKELDGHQSDETGSGEGENSNGGASNIGNTEDDQARLILKRKLQRNRTSFTNDQIDSLEKEFERTHYPDVFARERLAGKIGLPEARIQVWFSNRRAKWRREEKLRNQRRTPNSTGASATSSSTSATASLTDSPNSLSACSSLLSGSAGGPSVSTINGLSSPSTLSTNVNAPTLGAGIDSSESPTPIPHIRPSCTSDNDNGRQSEDCRRVCSPCPLGVGGHQNTHHIQSNGHAQGHALVPAISPRLNFNSGSFGAMYSNMHHTALSMSDSYGAVTPIPSFNHSAVGPLAPPSPIPQQGDLTPSSLYPCHMTLRPPPMAPAHHHIVPGDGGRPAGVGLGSGQSANLGASCSGSGYEVLSAYALPPPPMASSSAADSSFSAASSASANVTPHHTIAQESCPSPCSSASHFGVAHSSGFSSDPISPAVSSYAHMSYNYASSANTMTPSSASGTSAHVAPGKQQFFASCFYSPWV

Target ORF information:

RefSeq Version	NM_166789.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eyeless (ey), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166789.2

ORF Insert Sequence:

ATGCAGACAG CCACTCCTCT TAACTCTTCG GAAAGCGGTG GCGCAAGCAA CTCCGGGGAG 
GGTAGTGAAC AGGAGGCGAT TTACGAGAAG CTTCGGCTGT TAAATACTCA GCACGCTGCA 
GGACCAGGAC CACTGGAGCC TGCCAGAGCA GCGCCCTTGG TAGGTCAATC ACCCAACCAC 
CTAGGAACCC GATCCAGCCA CCCCCAGCTG GTGCACGGTA ACCATCAGGC ACTACAGCAG 
CATCAACAGC AGAGCTGGCC GCCCCGTCAC TATTCCGGAT CTTGGTACCC CACCTCTCTT 
AGCGAAATAC CCATCTCATC GGCTCCCAAT ATCGCATCCG TTACGGCGTA TGCATCAGGA 
CCTTCACTTG CTCACTCACT GAGTCCACCC AACGACATCG AAAGCCTGGC CAGTATCGGT 
CACCAGAGAA ACTGCCCCGT TGCAACGGAG GACATACATT TAAAAAAAGA ACTTGATGGT 
CATCAGTCCG ATGAAACGGG CTCCGGTGAA GGTGAAAACT CCAATGGTGG CGCTTCAAAT 
ATAGGAAACA CTGAGGATGA TCAAGCTCGG CTCATACTAA AAAGAAAGTT GCAACGCAAT 
CGAACATCTT TCACGAACGA CCAGATAGAC AGTCTTGAAA AAGAGTTTGA ACGAACACAC 
TATCCAGATG TTTTTGCCCG CGAACGTTTG GCTGGAAAGA TTGGGTTGCC AGAGGCAAGA 
ATTCAGGTTT GGTTCTCAAA CCGTCGAGCA AAATGGCGTC GCGAGGAGAA GCTGCGAAAC 
CAGCGAAGAA CACCAAATTC CACAGGAGCT AGTGCAACTT CTTCCTCTAC ATCGGCAACC 
GCCTCTTTGA CTGACAGCCC TAACAGCCTA AGTGCTTGTT CCTCGCTGCT GTCCGGATCA 
GCTGGGGGTC CCTCAGTCAG TACCATTAAT GGCTTATCGT CTCCAAGCAC ATTGTCTACT 
AATGTCAATG CTCCAACGCT TGGCGCTGGG ATCGATAGCT CTGAAAGCCC AACACCAATC 
CCGCACATTC GGCCTAGCTG CACCTCTGAC AATGACAATG GTCGTCAAAG TGAAGATTGC 
AGAAGAGTTT GTTCTCCATG CCCACTTGGC GTTGGCGGGC ATCAAAATAC TCATCATATC 
CAGAGCAATG GTCACGCCCA AGGTCATGCA CTTGTTCCTG CCATTTCGCC ACGACTCAAT 
TTTAATAGTG GTAGTTTCGG CGCGATGTAC TCCAACATGC ATCATACGGC GTTATCCATG 
AGCGATTCAT ATGGGGCGGT TACGCCGATT CCGAGCTTTA ACCACTCAGC TGTCGGTCCG 
CTGGCTCCGC CATCGCCAAT ACCGCAACAG GGCGATCTTA CCCCTTCCTC GTTATATCCG 
TGCCACATGA CCCTACGACC CCCTCCGATG GCTCCCGCTC ACCATCACAT CGTGCCGGGT 
GACGGTGGCA GACCTGCGGG CGTTGGCCTA GGCAGTGGCC AATCTGCGAA TTTGGGAGCA 
AGCTGCAGCG GATCGGGATA CGAAGTGCTA TCTGCCTACG CGTTGCCACC GCCCCCTATG 
GCGTCGAGCT CTGCTGCTGA TTCAAGCTTC TCAGCCGCGT CCAGTGCCAG CGCTAATGTG 
ACCCCACATC ACACCATAGC CCAAGAATCA TGCCCCTCTC CGTGTTCAAG CGCGAGCCAC 
TTTGGAGTTG CTCACAGTTC TGGGTTTTCG TCCGACCCGA TTTCACCGGC TGTATCTTCG 
TATGCACATA TGAGCTACAA TTACGCGTCG TCCGCTAACA CCATGACGCC TTCCTCCGCC 
AGCGGCACAT CAGCACACGT GGCCCCGGGA AAACAACAGT TCTTCGCCTC CTGTTTCTAC 
TCACCGTGGG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ey ( NM_001014693.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014693.1 Drosophila melanogaster eyeless (ey), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa00292
Clone ID Related Accession (Same CDS sequence)	NM_001014693.2
Accession Version	NM_001014693.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eyeless, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_001014693.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTACAT TGCAACCAAC TCCAACTGCT ATAGGCACCG TGGTTCCCCC ATGGTCAGCG 
GGAACATTGA TAGAGCGCCT GCCGTCTTTA GAAGACATGG CTCACAAGGA CAATGTGATT 
GCAATGAGAA ATTTACCCTG TTTGGGTACC GCTGGTGGTA GCGGACTAGG CGGTATTGCT 
GGGAAACCTT CCCCTACTAT GGAAGCCGTG GAAGCCAGCA CAGCGTCACA TCCCCATTCG 
ACATCTTCAT ACTTCGCCAC AACTTACTAC CATTTAACCG ATGATGAATG TCACAGTGGA 
GTAAATCAGC TGGGTGGCGT TTTTGTTGGA GGAAGGCCTT TGCCAGATTC AACACGGCAA 
AAAATTGTCG AACTGGCACA TTCTGGAGCT CGGCCATGTG ATATTTCTCG AATTCTGCAA 
GTATCAAATG GATGTGTGAG CAAAATTCTC GGGAGGTATT ATGAAACAGG AAGCATACGA 
CCACGTGCTA TCGGAGGATC CAAGCCACGT GTGGCCACAG CCGAAGTCGT TAGCAAAATT 
TCGCAGTACA AACGCGAGTG TCCTAGCATA TTTGCTTGGG AAATTCGGGA TAGATTACTT 
CAGGAGAACG TTTGTACTAA CGATAATATA CCAAGTGTGT CCTCAATAAA CCGTGTATTG 
AGAAACTTGG CTGCGCAAAA GGAGCAGCAA AGCACGGGAT CCGGGAGCTC CAGCACATCC 
GCCGGCAACT CAATCAGCGC AAAAGTGTCT GTCAGCATCG GTGGCAACGT GAGCAATGTG 
GCAAGCGGAT CGAGAGGCAC GTTGAGCTCT TCCACCGATC TTATGCAGAC AGCCACTCCT 
CTTAACTCTT CGGAAAGCGG TGGCGCAAGC AACTCCGGGG AGGGTAGTGA ACAGGAGGCG 
ATTTACGAGA AGCTTCGGCT GTTAAATACT CAGCACGCTG CAGGACCAGG ACCACTGGAG 
CCTGCCAGAG CAGCGCCCTT GGTAGGTCAA TCACCCAACC ACCTAGGAAC CCGATCCAGC 
CACCCCCAGC TGGTGCACGG TAACCATCAG GCACTACAGC AGCATCAACA GCAGAGCTGG 
CCGCCCCGTC ACTATTCCGG ATCTTGGTAC CCCACCTCTC TTAGCGAAAT ACCCATCTCA 
TCGGCTCCCA ATATCGCATC CGTTACGGCG TATGCATCAG GACCTTCACT TGCTCACTCA 
CTGAGTCCAC CCAACGACAT CGAAAGCCTG GCCAGTATCG GTCACCAGAG AAACTGCCCC 
GTTGCAACGG AGGACATACA TTTAAAAAAA GAACTTGATG GTCATCAGTC CGATGAAACG 
GGCTCCGGTG AAGGTGAAAA CTCCAATGGT GGCGCTTCAA ATATAGGAAA CACTGAGGAT 
GATCAAGCTC GGCTCATACT AAAAAGAAAG TTGCAACGCA ATCGAACATC TTTCACGAAC 
GACCAGATAG ACAGTCTTGA AAAAGAGTTT GAACGAACAC ACTATCCAGA TGTTTTTGCC 
CGCGAACGTT TGGCTGGAAA GATTGGGTTG CCAGAGGCAA GAATTCAGGT TTGGTTCTCA 
AACCGTCGAG CAAAATGGCG TCGCGAGGAG AAGCTGCGAA ACCAGCGAAG AACACCAAAT 
TCCACAGGAG CTAGTGCAAC TTCTTCCTCT ACATCGGCAA CCGCCTCTTT GACTGACAGC 
CCTAACAGCC TAAGTGCTTG TTCCTCGCTG CTGTCCGGAT CAGCTGGGGG TCCCTCAGTC 
AGTACCATTA ATGGCTTATC GTCTCCAAGC ACATTGTCTA CTAATGTCAA TGCTCCAACG 
CTTGGCGCTG GGATCGATAG CTCTGAAAGC CCAACACCAA TCCCGCACAT TCGGCCTAGC 
TGCACCTCTG ACAATGACAA TGGTCGTCAA AGTGAAGATT GCAGAAGAGT TTGTTCTCCA 
TGCCCACTTG GCGTTGGCGG GCATCAAAAT ACTCATCATA TCCAGAGCAA TGGTCACGCC 
CAAGGTCATG CACTTGTTCC TGCCATTTCG CCACGACTCA ATTTTAATAG TGGTAGTTTC 
GGCGCGATGT ACTCCAACAT GCATCATACG GCGTTATCCA TGAGCGATTC ATATGGGGCG 
GTTACGCCGA TTCCGAGCTT TAACCACTCA GCTGTCGGTC CGCTGGCTCC GCCATCGCCA 
ATACCGCAAC AGGGCGATCT TACCCCTTCC TCGTTATATC CGTGCCACAT GACCCTACGA 
CCCCCTCCGA TGGCTCCCGC TCACCATCAC ATCGTGCCGG GTGACGGTGG CAGACCTGCG 
GGCGTTGGCC TAGGCAGTGG CCAATCTGCG AATTTGGGAG CAAGCTGCAG CGGATCGGGA 
TACGAAGTGC TATCTGCCTA CGCGTTGCCA CCGCCCCCTA TGGCGTCGAG CTCTGCTGCT 
GATTCAAGCT TCTCAGCCGC GTCCAGTGCC AGCGCTAATG TGACCCCACA TCACACCATA 
GCCCAAGAAT CATGCCCCTC TCCGTGTTCA AGCGCGAGCC ACTTTGGAGT TGCTCACAGT 
TCTGGGTTTT CGTCCGACCC GATTTCACCG GCTGTATCTT CGTATGCACA TATGAGCTAC 
AATTACGCGT CGTCCGCTAA CACCATGACG CCTTCCTCCG CCAGCGGCAC ATCAGCACAC 
GTGGCCCCGG GAAAACAACA GTTCTTCGCC TCCTGTTTCT ACTCACCGTG GGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014693.1
CDS	515..3211
Translation	MFTLQPTPTAIGTVVPPWSAGTLIERLPSLEDMAHKDNVIAMRNLPCLGTAGGSGLGGIAGKPSPTMEAVEASTASHPHSTSSYFATTYYHLTDDECHSGVNQLGGVFVGGRPLPDSTRQKIVELAHSGARPCDISRILQVSNGCVSKILGRYYETGSIRPRAIGGSKPRVATAEVVSKISQYKRECPSIFAWEIRDRLLQENVCTNDNIPSVSSINRVLRNLAAQKEQQSTGSGSSSTSAGNSISAKVSVSIGGNVSNVASGSRGTLSSSTDLMQTATPLNSSESGGASNSGEGSEQEAIYEKLRLLNTQHAAGPGPLEPARAAPLVGQSPNHLGTRSSHPQLVHGNHQALQQHQQQSWPPRHYSGSWYPTSLSEIPISSAPNIASVTAYASGPSLAHSLSPPNDIESLASIGHQRNCPVATEDIHLKKELDGHQSDETGSGEGENSNGGASNIGNTEDDQARLILKRKLQRNRTSFTNDQIDSLEKEFERTHYPDVFARERLAGKIGLPEARIQVWFSNRRAKWRREEKLRNQRRTPNSTGASATSSSTSATASLTDSPNSLSACSSLLSGSAGGPSVSTINGLSSPSTLSTNVNAPTLGAGIDSSESPTPIPHIRPSCTSDNDNGRQSEDCRRVCSPCPLGVGGHQNTHHIQSNGHAQGHALVPAISPRLNFNSGSFGAMYSNMHHTALSMSDSYGAVTPIPSFNHSAVGPLAPPSPIPQQGDLTPSSLYPCHMTLRPPPMAPAHHHIVPGDGGRPAGVGLGSGQSANLGASCSGSGYEVLSAYALPPPPMASSSAADSSFSAASSASANVTPHHTIAQESCPSPCSSASHFGVAHSSGFSSDPISPAVSSYAHMSYNYASSANTMTPSSASGTSAHVAPGKQQFFASCFYSPWV

Target ORF information:

RefSeq Version	NM_001014693.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eyeless (ey), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014693.2

ORF Insert Sequence:

ATGTTTACAT TGCAACCAAC TCCAACTGCT ATAGGCACCG TGGTTCCCCC ATGGTCAGCG 
GGAACATTGA TAGAGCGCCT GCCGTCTTTA GAAGACATGG CTCACAAGGA CAATGTGATT 
GCAATGAGAA ATTTACCCTG TTTGGGTACC GCTGGTGGTA GCGGACTAGG CGGTATTGCT 
GGGAAACCTT CCCCTACTAT GGAAGCCGTG GAAGCCAGCA CAGCGTCACA TCCCCATTCG 
ACATCTTCAT ACTTCGCCAC AACTTACTAC CATTTAACCG ATGATGAATG TCACAGTGGA 
GTAAATCAGC TGGGTGGCGT TTTTGTTGGA GGAAGGCCTT TGCCAGATTC AACACGGCAA 
AAAATTGTCG AACTGGCACA TTCTGGAGCT CGGCCATGTG ATATTTCTCG AATTCTGCAA 
GTATCAAATG GATGTGTGAG CAAAATTCTC GGGAGGTATT ATGAAACAGG AAGCATACGA 
CCACGTGCTA TCGGAGGATC CAAGCCACGT GTGGCCACAG CCGAAGTCGT TAGCAAAATT 
TCGCAGTACA AACGCGAGTG TCCTAGCATA TTTGCTTGGG AAATTCGGGA TAGATTACTT 
CAGGAGAACG TTTGTACTAA CGATAATATA CCAAGTGTGT CCTCAATAAA CCGTGTATTG 
AGAAACTTGG CTGCGCAAAA GGAGCAGCAA AGCACGGGAT CCGGGAGCTC CAGCACATCC 
GCCGGCAACT CAATCAGCGC AAAAGTGTCT GTCAGCATCG GTGGCAACGT GAGCAATGTG 
GCAAGCGGAT CGAGAGGCAC GTTGAGCTCT TCCACCGATC TTATGCAGAC AGCCACTCCT 
CTTAACTCTT CGGAAAGCGG TGGCGCAAGC AACTCCGGGG AGGGTAGTGA ACAGGAGGCG 
ATTTACGAGA AGCTTCGGCT GTTAAATACT CAGCACGCTG CAGGACCAGG ACCACTGGAG 
CCTGCCAGAG CAGCGCCCTT GGTAGGTCAA TCACCCAACC ACCTAGGAAC CCGATCCAGC 
CACCCCCAGC TGGTGCACGG TAACCATCAG GCACTACAGC AGCATCAACA GCAGAGCTGG 
CCGCCCCGTC ACTATTCCGG ATCTTGGTAC CCCACCTCTC TTAGCGAAAT ACCCATCTCA 
TCGGCTCCCA ATATCGCATC CGTTACGGCG TATGCATCAG GACCTTCACT TGCTCACTCA 
CTGAGTCCAC CCAACGACAT CGAAAGCCTG GCCAGTATCG GTCACCAGAG AAACTGCCCC 
GTTGCAACGG AGGACATACA TTTAAAAAAA GAACTTGATG GTCATCAGTC CGATGAAACG 
GGCTCCGGTG AAGGTGAAAA CTCCAATGGT GGCGCTTCAA ATATAGGAAA CACTGAGGAT 
GATCAAGCTC GGCTCATACT AAAAAGAAAG TTGCAACGCA ATCGAACATC TTTCACGAAC 
GACCAGATAG ACAGTCTTGA AAAAGAGTTT GAACGAACAC ACTATCCAGA TGTTTTTGCC 
CGCGAACGTT TGGCTGGAAA GATTGGGTTG CCAGAGGCAA GAATTCAGGT TTGGTTCTCA 
AACCGTCGAG CAAAATGGCG TCGCGAGGAG AAGCTGCGAA ACCAGCGAAG AACACCAAAT 
TCCACAGGAG CTAGTGCAAC TTCTTCCTCT ACATCGGCAA CCGCCTCTTT GACTGACAGC 
CCTAACAGCC TAAGTGCTTG TTCCTCGCTG CTGTCCGGAT CAGCTGGGGG TCCCTCAGTC 
AGTACCATTA ATGGCTTATC GTCTCCAAGC ACATTGTCTA CTAATGTCAA TGCTCCAACG 
CTTGGCGCTG GGATCGATAG CTCTGAAAGC CCAACACCAA TCCCGCACAT TCGGCCTAGC 
TGCACCTCTG ACAATGACAA TGGTCGTCAA AGTGAAGATT GCAGAAGAGT TTGTTCTCCA 
TGCCCACTTG GCGTTGGCGG GCATCAAAAT ACTCATCATA TCCAGAGCAA TGGTCACGCC 
CAAGGTCATG CACTTGTTCC TGCCATTTCG CCACGACTCA ATTTTAATAG TGGTAGTTTC 
GGCGCGATGT ACTCCAACAT GCATCATACG GCGTTATCCA TGAGCGATTC ATATGGGGCG 
GTTACGCCGA TTCCGAGCTT TAACCACTCA GCTGTCGGTC CGCTGGCTCC GCCATCGCCA 
ATACCGCAAC AGGGCGATCT TACCCCTTCC TCGTTATATC CGTGCCACAT GACCCTACGA 
CCCCCTCCGA TGGCTCCCGC TCACCATCAC ATCGTGCCGG GTGACGGTGG CAGACCTGCG 
GGCGTTGGCC TAGGCAGTGG CCAATCTGCG AATTTGGGAG CAAGCTGCAG CGGATCGGGA 
TACGAAGTGC TATCTGCCTA CGCGTTGCCA CCGCCCCCTA TGGCGTCGAG CTCTGCTGCT 
GATTCAAGCT TCTCAGCCGC GTCCAGTGCC AGCGCTAATG TGACCCCACA TCACACCATA 
GCCCAAGAAT CATGCCCCTC TCCGTGTTCA AGCGCGAGCC ACTTTGGAGT TGCTCACAGT 
TCTGGGTTTT CGTCCGACCC GATTTCACCG GCTGTATCTT CGTATGCACA TATGAGCTAC 
AATTACGCGT CGTCCGCTAA CACCATGACG CCTTCCTCCG CCAGCGGCAC ATCAGCACAC 
GTGGCCCCGG GAAAACAACA GTTCTTCGCC TCCTGTTTCT ACTCACCGTG GGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ey ( NM_079889.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524628.2 Drosophila melanogaster eyeless (ey), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00293
Clone ID Related Accession (Same CDS sequence)	NM_079889.3
Accession Version	NM_079889.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eyeless, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_079889.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTACAT TGCAACCAAC TCCAACTGCT ATAGGCACCG TGGTTCCCCC ATGGTCAGCG 
GGAACATTGA TAGAGCGCCT GCCGTCTTTA GAAGACATGG CTCACAAGGG TCACAGTGGA 
GTAAATCAGC TGGGTGGCGT TTTTGTTGGA GGAAGGCCTT TGCCAGATTC AACACGGCAA 
AAAATTGTCG AACTGGCACA TTCTGGAGCT CGGCCATGTG ATATTTCTCG AATTCTGCAA 
GTATCAAATG GATGTGTGAG CAAAATTCTC GGGAGGTATT ATGAAACAGG AAGCATACGA 
CCACGTGCTA TCGGAGGATC CAAGCCACGT GTGGCCACAG CCGAAGTCGT TAGCAAAATT 
TCGCAGTACA AACGCGAGTG TCCTAGCATA TTTGCTTGGG AAATTCGGGA TAGATTACTT 
CAGGAGAACG TTTGTACTAA CGATAATATA CCAAGTGTGT CCTCAATAAA CCGTGTATTG 
AGAAACTTGG CTGCGCAAAA GGAGCAGCAA AGCACGGGAT CCGGGAGCTC CAGCACATCC 
GCCGGCAACT CAATCAGCGC AAAAGTGTCT GTCAGCATCG GTGGCAACGT GAGCAATGTG 
GCAAGCGGAT CGAGAGGCAC GTTGAGCTCT TCCACCGATC TTATGCAGAC AGCCACTCCT 
CTTAACTCTT CGGAAAGCGG TGGCGCAAGC AACTCCGGGG AGGGTAGTGA ACAGGAGGCG 
ATTTACGAGA AGCTTCGGCT GTTAAATACT CAGCACGCTG CAGGACCAGG ACCACTGGAG 
CCTGCCAGAG CAGCGCCCTT GGTAGGTCAA TCACCCAACC ACCTAGGAAC CCGATCCAGC 
CACCCCCAGC TGGTGCACGG TAACCATCAG GCACTACAGC AGCATCAACA GCAGAGCTGG 
CCGCCCCGTC ACTATTCCGG ATCTTGGTAC CCCACCTCTC TTAGCGAAAT ACCCATCTCA 
TCGGCTCCCA ATATCGCATC CGTTACGGCG TATGCATCAG GACCTTCACT TGCTCACTCA 
CTGAGTCCAC CCAACGACAT CGAAAGCCTG GCCAGTATCG GTCACCAGAG AAACTGCCCC 
GTTGCAACGG AGGACATACA TTTAAAAAAA GAACTTGATG GTCATCAGTC CGATGAAACG 
GGCTCCGGTG AAGGTGAAAA CTCCAATGGT GGCGCTTCAA ATATAGGAAA CACTGAGGAT 
GATCAAGCTC GGCTCATACT AAAAAGAAAG TTGCAACGCA ATCGAACATC TTTCACGAAC 
GACCAGATAG ACAGTCTTGA AAAAGAGTTT GAACGAACAC ACTATCCAGA TGTTTTTGCC 
CGCGAACGTT TGGCTGGAAA GATTGGGTTG CCAGAGGCAA GAATTCAGGT TTGGTTCTCA 
AACCGTCGAG CAAAATGGCG TCGCGAGGAG AAGCTGCGAA ACCAGCGAAG AACACCAAAT 
TCCACAGGAG CTAGTGCAAC TTCTTCCTCT ACATCGGCAA CCGCCTCTTT GACTGACAGC 
CCTAACAGCC TAAGTGCTTG TTCCTCGCTG CTGTCCGGAT CAGCTGGGGG TCCCTCAGTC 
AGTACCATTA ATGGCTTATC GTCTCCAAGC ACATTGTCTA CTAATGTCAA TGCTCCAACG 
CTTGGCGCTG GGATCGATAG CTCTGAAAGC CCAACACCAA TCCCGCACAT TCGGCCTAGC 
TGCACCTCTG ACAATGACAA TGGTCGTCAA AGTGAAGATT GCAGAAGAGT TTGTTCTCCA 
TGCCCACTTG GCGTTGGCGG GCATCAAAAT ACTCATCATA TCCAGAGCAA TGGTCACGCC 
CAAGGTCATG CACTTGTTCC TGCCATTTCG CCACGACTCA ATTTTAATAG TGGTAGTTTC 
GGCGCGATGT ACTCCAACAT GCATCATACG GCGTTATCCA TGAGCGATTC ATATGGGGCG 
GTTACGCCGA TTCCGAGCTT TAACCACTCA GCTGTCGGTC CGCTGGCTCC GCCATCGCCA 
ATACCGCAAC AGGGCGATCT TACCCCTTCC TCGTTATATC CGTGCCACAT GACCCTACGA 
CCCCCTCCGA TGGCTCCCGC TCACCATCAC ATCGTGCCGG GTGACGGTGG CAGACCTGCG 
GGCGTTGGCC TAGGCAGTGG CCAATCTGCG AATTTGGGAG CAAGCTGCAG CGGATCGGGA 
TACGAAGTGC TATCTGCCTA CGCGTTGCCA CCGCCCCCTA TGGCGTCGAG CTCTGCTGCT 
GATTCAAGCT TCTCAGCCGC GTCCAGTGCC AGCGCTAATG TGACCCCACA TCACACCATA 
GCCCAAGAAT CATGCCCCTC TCCGTGTTCA AGCGCGAGCC ACTTTGGAGT TGCTCACAGT 
TCTGGGTTTT CGTCCGACCC GATTTCACCG GCTGTATCTT CGTATGCACA TATGAGCTAC 
AATTACGCGT CGTCCGCTAA CACCATGACG CCTTCCTCCG CCAGCGGCAC ATCAGCACAC 
GTGGCCCCGG GAAAACAACA GTTCTTCGCC TCCTGTTTCT ACTCACCGTG GGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524628.2
CDS	515..3031
Translation	MFTLQPTPTAIGTVVPPWSAGTLIERLPSLEDMAHKGHSGVNQLGGVFVGGRPLPDSTRQKIVELAHSGARPCDISRILQVSNGCVSKILGRYYETGSIRPRAIGGSKPRVATAEVVSKISQYKRECPSIFAWEIRDRLLQENVCTNDNIPSVSSINRVLRNLAAQKEQQSTGSGSSSTSAGNSISAKVSVSIGGNVSNVASGSRGTLSSSTDLMQTATPLNSSESGGASNSGEGSEQEAIYEKLRLLNTQHAAGPGPLEPARAAPLVGQSPNHLGTRSSHPQLVHGNHQALQQHQQQSWPPRHYSGSWYPTSLSEIPISSAPNIASVTAYASGPSLAHSLSPPNDIESLASIGHQRNCPVATEDIHLKKELDGHQSDETGSGEGENSNGGASNIGNTEDDQARLILKRKLQRNRTSFTNDQIDSLEKEFERTHYPDVFARERLAGKIGLPEARIQVWFSNRRAKWRREEKLRNQRRTPNSTGASATSSSTSATASLTDSPNSLSACSSLLSGSAGGPSVSTINGLSSPSTLSTNVNAPTLGAGIDSSESPTPIPHIRPSCTSDNDNGRQSEDCRRVCSPCPLGVGGHQNTHHIQSNGHAQGHALVPAISPRLNFNSGSFGAMYSNMHHTALSMSDSYGAVTPIPSFNHSAVGPLAPPSPIPQQGDLTPSSLYPCHMTLRPPPMAPAHHHIVPGDGGRPAGVGLGSGQSANLGASCSGSGYEVLSAYALPPPPMASSSAADSSFSAASSASANVTPHHTIAQESCPSPCSSASHFGVAHSSGFSSDPISPAVSSYAHMSYNYASSANTMTPSSASGTSAHVAPGKQQFFASCFYSPWV

Target ORF information:

RefSeq Version	NM_079889.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eyeless (ey), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079889.3

ORF Insert Sequence:

ATGTTTACAT TGCAACCAAC TCCAACTGCT ATAGGCACCG TGGTTCCCCC ATGGTCAGCG 
GGAACATTGA TAGAGCGCCT GCCGTCTTTA GAAGACATGG CTCACAAGGG TCACAGTGGA 
GTAAATCAGC TGGGTGGCGT TTTTGTTGGA GGAAGGCCTT TGCCAGATTC AACACGGCAA 
AAAATTGTCG AACTGGCACA TTCTGGAGCT CGGCCATGTG ATATTTCTCG AATTCTGCAA 
GTATCAAATG GATGTGTGAG CAAAATTCTC GGGAGGTATT ATGAAACAGG AAGCATACGA 
CCACGTGCTA TCGGAGGATC CAAGCCACGT GTGGCCACAG CCGAAGTCGT TAGCAAAATT 
TCGCAGTACA AACGCGAGTG TCCTAGCATA TTTGCTTGGG AAATTCGGGA TAGATTACTT 
CAGGAGAACG TTTGTACTAA CGATAATATA CCAAGTGTGT CCTCAATAAA CCGTGTATTG 
AGAAACTTGG CTGCGCAAAA GGAGCAGCAA AGCACGGGAT CCGGGAGCTC CAGCACATCC 
GCCGGCAACT CAATCAGCGC AAAAGTGTCT GTCAGCATCG GTGGCAACGT GAGCAATGTG 
GCAAGCGGAT CGAGAGGCAC GTTGAGCTCT TCCACCGATC TTATGCAGAC AGCCACTCCT 
CTTAACTCTT CGGAAAGCGG TGGCGCAAGC AACTCCGGGG AGGGTAGTGA ACAGGAGGCG 
ATTTACGAGA AGCTTCGGCT GTTAAATACT CAGCACGCTG CAGGACCAGG ACCACTGGAG 
CCTGCCAGAG CAGCGCCCTT GGTAGGTCAA TCACCCAACC ACCTAGGAAC CCGATCCAGC 
CACCCCCAGC TGGTGCACGG TAACCATCAG GCACTACAGC AGCATCAACA GCAGAGCTGG 
CCGCCCCGTC ACTATTCCGG ATCTTGGTAC CCCACCTCTC TTAGCGAAAT ACCCATCTCA 
TCGGCTCCCA ATATCGCATC CGTTACGGCG TATGCATCAG GACCTTCACT TGCTCACTCA 
CTGAGTCCAC CCAACGACAT CGAAAGCCTG GCCAGTATCG GTCACCAGAG AAACTGCCCC 
GTTGCAACGG AGGACATACA TTTAAAAAAA GAACTTGATG GTCATCAGTC CGATGAAACG 
GGCTCCGGTG AAGGTGAAAA CTCCAATGGT GGCGCTTCAA ATATAGGAAA CACTGAGGAT 
GATCAAGCTC GGCTCATACT AAAAAGAAAG TTGCAACGCA ATCGAACATC TTTCACGAAC 
GACCAGATAG ACAGTCTTGA AAAAGAGTTT GAACGAACAC ACTATCCAGA TGTTTTTGCC 
CGCGAACGTT TGGCTGGAAA GATTGGGTTG CCAGAGGCAA GAATTCAGGT TTGGTTCTCA 
AACCGTCGAG CAAAATGGCG TCGCGAGGAG AAGCTGCGAA ACCAGCGAAG AACACCAAAT 
TCCACAGGAG CTAGTGCAAC TTCTTCCTCT ACATCGGCAA CCGCCTCTTT GACTGACAGC 
CCTAACAGCC TAAGTGCTTG TTCCTCGCTG CTGTCCGGAT CAGCTGGGGG TCCCTCAGTC 
AGTACCATTA ATGGCTTATC GTCTCCAAGC ACATTGTCTA CTAATGTCAA TGCTCCAACG 
CTTGGCGCTG GGATCGATAG CTCTGAAAGC CCAACACCAA TCCCGCACAT TCGGCCTAGC 
TGCACCTCTG ACAATGACAA TGGTCGTCAA AGTGAAGATT GCAGAAGAGT TTGTTCTCCA 
TGCCCACTTG GCGTTGGCGG GCATCAAAAT ACTCATCATA TCCAGAGCAA TGGTCACGCC 
CAAGGTCATG CACTTGTTCC TGCCATTTCG CCACGACTCA ATTTTAATAG TGGTAGTTTC 
GGCGCGATGT ACTCCAACAT GCATCATACG GCGTTATCCA TGAGCGATTC ATATGGGGCG 
GTTACGCCGA TTCCGAGCTT TAACCACTCA GCTGTCGGTC CGCTGGCTCC GCCATCGCCA 
ATACCGCAAC AGGGCGATCT TACCCCTTCC TCGTTATATC CGTGCCACAT GACCCTACGA 
CCCCCTCCGA TGGCTCCCGC TCACCATCAC ATCGTGCCGG GTGACGGTGG CAGACCTGCG 
GGCGTTGGCC TAGGCAGTGG CCAATCTGCG AATTTGGGAG CAAGCTGCAG CGGATCGGGA 
TACGAAGTGC TATCTGCCTA CGCGTTGCCA CCGCCCCCTA TGGCGTCGAG CTCTGCTGCT 
GATTCAAGCT TCTCAGCCGC GTCCAGTGCC AGCGCTAATG TGACCCCACA TCACACCATA 
GCCCAAGAAT CATGCCCCTC TCCGTGTTCA AGCGCGAGCC ACTTTGGAGT TGCTCACAGT 
TCTGGGTTTT CGTCCGACCC GATTTCACCG GCTGTATCTT CGTATGCACA TATGAGCTAC 
AATTACGCGT CGTCCGCTAA CACCATGACG CCTTCCTCCG CCAGCGGCAC ATCAGCACAC 
GTGGCCCCGG GAAAACAACA GTTCTTCGCC TCCTGTTTCT ACTCACCGTG GGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ey ( NM_001014694.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014694.1 Drosophila melanogaster eyeless (ey), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa00294
Clone ID Related Accession (Same CDS sequence)	NM_001014694.2
Accession Version	NM_001014694.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eyeless, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_001014694.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGAAATT TACCCTGTTT GGGTACCGCT GGTGGTAGCG GACTAGGCGG TATTGCTGGG 
AAACCTTCCC CTACTATGGA AGCCGTGGAA GCCAGCACAG CGTCACATCC CCATTCGACA 
TCTTCATACT TCGCCACAAC TTACTACCAT TTAACCGATG ATGAATGTCA CAGTGGAGTA 
AATCAGCTGG GTGGCGTTTT TGTTGGAGGA AGGCCTTTGC CAGATTCAAC ACGGCAAAAA 
ATTGTCGAAC TGGCACATTC TGGAGCTCGG CCATGTGATA TTTCTCGAAT TCTGCAAGTA 
TCAAATGGAT GTGTGAGCAA AATTCTCGGG AGGTATTATG AAACAGGAAG CATACGACCA 
CGTGCTATCG GAGGATCCAA GCCACGTGTG GCCACAGCCG AAGTCGTTAG CAAAATTTCG 
CAGTACAAAC GCGAGTGTCC TAGCATATTT GCTTGGGAAA TTCGGGATAG ATTACTTCAG 
GAGAACGTTT GTACTAACGA TAATATACCA AGTGTGTCCT CAATAAACCG TGTATTGAGA 
AACTTGGCTG CGCAAAAGGA GCAGCAAAGC ACGGGATCCG GGAGCTCCAG CACATCCGCC 
GGCAACTCAA TCAGCGCAAA AGTGTCTGTC AGCATCGGTG GCAACGTGAG CAATGTGGCA 
AGCGGATCGA GAGGCACGTT GAGCTCTTCC ACCGATCTTA TGCAGACAGC CACTCCTCTT 
AACTCTTCGG AAAGCGGTGG CGCAAGCAAC TCCGGGGAGG GTAGTGAACA GGAGGCGATT 
TACGAGAAGC TTCGGCTGTT AAATACTCAG CACGCTGCAG GACCAGGACC ACTGGAGCCT 
GCCAGAGCAG CGCCCTTGGT AGGTCAATCA CCCAACCACC TAGGAACCCG ATCCAGCCAC 
CCCCAGCTGG TGCACGGTAA CCATCAGGCA CTACAGCAGC ATCAACAGCA GAGCTGGCCG 
CCCCGTCACT ATTCCGGATC TTGGTACCCC ACCTCTCTTA GCGAAATACC CATCTCATCG 
GCTCCCAATA TCGCATCCGT TACGGCGTAT GCATCAGGAC CTTCACTTGC TCACTCACTG 
AGTCCACCCA ACGACATCGA AAGCCTGGCC AGTATCGGTC ACCAGAGAAA CTGCCCCGTT 
GCAACGGAGG ACATACATTT AAAAAAAGAA CTTGATGGTC ATCAGTCCGA TGAAACGGGC 
TCCGGTGAAG GTGAAAACTC CAATGGTGGC GCTTCAAATA TAGGAAACAC TGAGGATGAT 
CAAGCTCGGC TCATACTAAA AAGAAAGTTG CAACGCAATC GAACATCTTT CACGAACGAC 
CAGATAGACA GTCTTGAAAA AGAGTTTGAA CGAACACACT ATCCAGATGT TTTTGCCCGC 
GAACGTTTGG CTGGAAAGAT TGGGTTGCCA GAGGCAAGAA TTCAGGTTTG GTTCTCAAAC 
CGTCGAGCAA AATGGCGTCG CGAGGAGAAG CTGCGAAACC AGCGAAGAAC ACCAAATTCC 
ACAGGAGCTA GTGCAACTTC TTCCTCTACA TCGGCAACCG CCTCTTTGAC TGACAGCCCT 
AACAGCCTAA GTGCTTGTTC CTCGCTGCTG TCCGGATCAG CTGGGGGTCC CTCAGTCAGT 
ACCATTAATG GCTTATCGTC TCCAAGCACA TTGTCTACTA ATGTCAATGC TCCAACGCTT 
GGCGCTGGGA TCGATAGCTC TGAAAGCCCA ACACCAATCC CGCACATTCG GCCTAGCTGC 
ACCTCTGACA ATGACAATGG TCGTCAAAGT GAAGATTGCA GAAGAGTTTG TTCTCCATGC 
CCACTTGGCG TTGGCGGGCA TCAAAATACT CATCATATCC AGAGCAATGG TCACGCCCAA 
GGTCATGCAC TTGTTCCTGC CATTTCGCCA CGACTCAATT TTAATAGTGG TAGTTTCGGC 
GCGATGTACT CCAACATGCA TCATACGGCG TTATCCATGA GCGATTCATA TGGGGCGGTT 
ACGCCGATTC CGAGCTTTAA CCACTCAGCT GTCGGTCCGC TGGCTCCGCC ATCGCCAATA 
CCGCAACAGG GCGATCTTAC CCCTTCCTCG TTATATCCGT GCCACATGAC CCTACGACCC 
CCTCCGATGG CTCCCGCTCA CCATCACATC GTGCCGGGTG ACGGTGGCAG ACCTGCGGGC 
GTTGGCCTAG GCAGTGGCCA ATCTGCGAAT TTGGGAGCAA GCTGCAGCGG ATCGGGATAC 
GAAGTGCTAT CTGCCTACGC GTTGCCACCG CCCCCTATGG CGTCGAGCTC TGCTGCTGAT 
TCAAGCTTCT CAGCCGCGTC CAGTGCCAGC GCTAATGTGA CCCCACATCA CACCATAGCC 
CAAGAATCAT GCCCCTCTCC GTGTTCAAGC GCGAGCCACT TTGGAGTTGC TCACAGTTCT 
GGGTTTTCGT CCGACCCGAT TTCACCGGCT GTATCTTCGT ATGCACATAT GAGCTACAAT 
TACGCGTCGT CCGCTAACAC CATGACGCCT TCCTCCGCCA GCGGCACATC AGCACACGTG 
GCCCCGGGAA AACAACAGTT CTTCGCCTCC TGTTTCTACT CACCGTGGGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014694.1
CDS	78..2651
Translation	MRNLPCLGTAGGSGLGGIAGKPSPTMEAVEASTASHPHSTSSYFATTYYHLTDDECHSGVNQLGGVFVGGRPLPDSTRQKIVELAHSGARPCDISRILQVSNGCVSKILGRYYETGSIRPRAIGGSKPRVATAEVVSKISQYKRECPSIFAWEIRDRLLQENVCTNDNIPSVSSINRVLRNLAAQKEQQSTGSGSSSTSAGNSISAKVSVSIGGNVSNVASGSRGTLSSSTDLMQTATPLNSSESGGASNSGEGSEQEAIYEKLRLLNTQHAAGPGPLEPARAAPLVGQSPNHLGTRSSHPQLVHGNHQALQQHQQQSWPPRHYSGSWYPTSLSEIPISSAPNIASVTAYASGPSLAHSLSPPNDIESLASIGHQRNCPVATEDIHLKKELDGHQSDETGSGEGENSNGGASNIGNTEDDQARLILKRKLQRNRTSFTNDQIDSLEKEFERTHYPDVFARERLAGKIGLPEARIQVWFSNRRAKWRREEKLRNQRRTPNSTGASATSSSTSATASLTDSPNSLSACSSLLSGSAGGPSVSTINGLSSPSTLSTNVNAPTLGAGIDSSESPTPIPHIRPSCTSDNDNGRQSEDCRRVCSPCPLGVGGHQNTHHIQSNGHAQGHALVPAISPRLNFNSGSFGAMYSNMHHTALSMSDSYGAVTPIPSFNHSAVGPLAPPSPIPQQGDLTPSSLYPCHMTLRPPPMAPAHHHIVPGDGGRPAGVGLGSGQSANLGASCSGSGYEVLSAYALPPPPMASSSAADSSFSAASSASANVTPHHTIAQESCPSPCSSASHFGVAHSSGFSSDPISPAVSSYAHMSYNYASSANTMTPSSASGTSAHVAPGKQQFFASCFYSPWV

Target ORF information:

RefSeq Version	NM_001014694.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eyeless (ey), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014694.2

ORF Insert Sequence:

ATGAGAAATT TACCCTGTTT GGGTACCGCT GGTGGTAGCG GACTAGGCGG TATTGCTGGG 
AAACCTTCCC CTACTATGGA AGCCGTGGAA GCCAGCACAG CGTCACATCC CCATTCGACA 
TCTTCATACT TCGCCACAAC TTACTACCAT TTAACCGATG ATGAATGTCA CAGTGGAGTA 
AATCAGCTGG GTGGCGTTTT TGTTGGAGGA AGGCCTTTGC CAGATTCAAC ACGGCAAAAA 
ATTGTCGAAC TGGCACATTC TGGAGCTCGG CCATGTGATA TTTCTCGAAT TCTGCAAGTA 
TCAAATGGAT GTGTGAGCAA AATTCTCGGG AGGTATTATG AAACAGGAAG CATACGACCA 
CGTGCTATCG GAGGATCCAA GCCACGTGTG GCCACAGCCG AAGTCGTTAG CAAAATTTCG 
CAGTACAAAC GCGAGTGTCC TAGCATATTT GCTTGGGAAA TTCGGGATAG ATTACTTCAG 
GAGAACGTTT GTACTAACGA TAATATACCA AGTGTGTCCT CAATAAACCG TGTATTGAGA 
AACTTGGCTG CGCAAAAGGA GCAGCAAAGC ACGGGATCCG GGAGCTCCAG CACATCCGCC 
GGCAACTCAA TCAGCGCAAA AGTGTCTGTC AGCATCGGTG GCAACGTGAG CAATGTGGCA 
AGCGGATCGA GAGGCACGTT GAGCTCTTCC ACCGATCTTA TGCAGACAGC CACTCCTCTT 
AACTCTTCGG AAAGCGGTGG CGCAAGCAAC TCCGGGGAGG GTAGTGAACA GGAGGCGATT 
TACGAGAAGC TTCGGCTGTT AAATACTCAG CACGCTGCAG GACCAGGACC ACTGGAGCCT 
GCCAGAGCAG CGCCCTTGGT AGGTCAATCA CCCAACCACC TAGGAACCCG ATCCAGCCAC 
CCCCAGCTGG TGCACGGTAA CCATCAGGCA CTACAGCAGC ATCAACAGCA GAGCTGGCCG 
CCCCGTCACT ATTCCGGATC TTGGTACCCC ACCTCTCTTA GCGAAATACC CATCTCATCG 
GCTCCCAATA TCGCATCCGT TACGGCGTAT GCATCAGGAC CTTCACTTGC TCACTCACTG 
AGTCCACCCA ACGACATCGA AAGCCTGGCC AGTATCGGTC ACCAGAGAAA CTGCCCCGTT 
GCAACGGAGG ACATACATTT AAAAAAAGAA CTTGATGGTC ATCAGTCCGA TGAAACGGGC 
TCCGGTGAAG GTGAAAACTC CAATGGTGGC GCTTCAAATA TAGGAAACAC TGAGGATGAT 
CAAGCTCGGC TCATACTAAA AAGAAAGTTG CAACGCAATC GAACATCTTT CACGAACGAC 
CAGATAGACA GTCTTGAAAA AGAGTTTGAA CGAACACACT ATCCAGATGT TTTTGCCCGC 
GAACGTTTGG CTGGAAAGAT TGGGTTGCCA GAGGCAAGAA TTCAGGTTTG GTTCTCAAAC 
CGTCGAGCAA AATGGCGTCG CGAGGAGAAG CTGCGAAACC AGCGAAGAAC ACCAAATTCC 
ACAGGAGCTA GTGCAACTTC TTCCTCTACA TCGGCAACCG CCTCTTTGAC TGACAGCCCT 
AACAGCCTAA GTGCTTGTTC CTCGCTGCTG TCCGGATCAG CTGGGGGTCC CTCAGTCAGT 
ACCATTAATG GCTTATCGTC TCCAAGCACA TTGTCTACTA ATGTCAATGC TCCAACGCTT 
GGCGCTGGGA TCGATAGCTC TGAAAGCCCA ACACCAATCC CGCACATTCG GCCTAGCTGC 
ACCTCTGACA ATGACAATGG TCGTCAAAGT GAAGATTGCA GAAGAGTTTG TTCTCCATGC 
CCACTTGGCG TTGGCGGGCA TCAAAATACT CATCATATCC AGAGCAATGG TCACGCCCAA 
GGTCATGCAC TTGTTCCTGC CATTTCGCCA CGACTCAATT TTAATAGTGG TAGTTTCGGC 
GCGATGTACT CCAACATGCA TCATACGGCG TTATCCATGA GCGATTCATA TGGGGCGGTT 
ACGCCGATTC CGAGCTTTAA CCACTCAGCT GTCGGTCCGC TGGCTCCGCC ATCGCCAATA 
CCGCAACAGG GCGATCTTAC CCCTTCCTCG TTATATCCGT GCCACATGAC CCTACGACCC 
CCTCCGATGG CTCCCGCTCA CCATCACATC GTGCCGGGTG ACGGTGGCAG ACCTGCGGGC 
GTTGGCCTAG GCAGTGGCCA ATCTGCGAAT TTGGGAGCAA GCTGCAGCGG ATCGGGATAC 
GAAGTGCTAT CTGCCTACGC GTTGCCACCG CCCCCTATGG CGTCGAGCTC TGCTGCTGAT 
TCAAGCTTCT CAGCCGCGTC CAGTGCCAGC GCTAATGTGA CCCCACATCA CACCATAGCC 
CAAGAATCAT GCCCCTCTCC GTGTTCAAGC GCGAGCCACT TTGGAGTTGC TCACAGTTCT 
GGGTTTTCGT CCGACCCGAT TTCACCGGCT GTATCTTCGT ATGCACATAT GAGCTACAAT 
TACGCGTCGT CCGCTAACAC CATGACGCCT TCCTCCGCCA GCGGCACATC AGCACACGTG 
GCCCCGGGAA AACAACAGTT CTTCGCCTCC TGTTTCTACT CACCGTGGGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ey cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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