Csk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Csk
Entrez Gene ID	41398
Full Name	C-terminal Src kinase
Synonyms	CG17309,CG32852,CG42317,CSK,DCsk,Dmel\CG42317,Dmel_CG17309,Dmel_CG32852,csk,d-Csk,dCSK,dCsk,dcsk,flik,l(3)S017909,l(3)j1D8
General protein information	Preferred Names C-terminal Src kinase Names CG42317 gene product from transcript CG42317-RI C-Src kinase C-terminal-Src kinase CG42317-PF CG42317-PG CG42317-PH CG42317-PI CG42317-PJ CG42317-PK CG42317-PL Csk-PF Csk-PG Csk-PH Csk-PI Csk-PJ Csk-PK Csk-PL drosophila C-terminal Src kinase flik lethal (3) S017909 lethal (3) j1D8
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 86E13-86E13

mRNA and Protein(s)

mRNA	Protein	Name
NM_169418.3	NP_731611.2	C-terminal Src kinase, isoform G
NM_169416.2	NP_731609.1	C-terminal Src kinase, isoform K
NM_001275547.1	NP_001262476.1	C-terminal Src kinase, isoform L
NM_141840.1	NP_650097.2	C-terminal Src kinase, isoform H
NM_176461.1	NP_788638.1	C-terminal Src kinase, isoform F
NM_169414.2	NP_731607.1	C-terminal Src kinase, isoform I
NM_169415.2	NP_731608.1	C-terminal Src kinase, isoform J

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Csk	NP_001025210.1
Drosophila melanogaster (fruit fly)	Csk	NP_788638.1
Homo sapiens (human)	CSK	NP_004374.1
Bos taurus (cattle)	CSK	NP_001068865.1
Danio rerio (zebrafish)	csk	NP_001071067.1
Xenopus tropicalis (tropical clawed frog)	csk	NP_001135615.1
Mus musculus (house mouse)	Csk	NP_031809.2
Gallus gallus (chicken)	CSK	NP_990756.1
Caenorhabditis elegans (roundworm)	csk-1	NP_001021778.1
Pan troglodytes (chimpanzee)	CSK	XP_001138334.3
	CSK	XP_001101521.1
Canis lupus familiaris (dog)	CSK	XP_005638681.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Csk and its SFK substrates impinge upon homeostatic control of NMJ function by regulating downstream expression or localization of FasII.
Title: C-terminal Src Kinase Gates Homeostatic Synaptic Plasticity and Regulates Fasciclin II Expression at the Drosophila Neuromuscular Junction.

dCsk is another upstream member of the network of genes that interact to regulate Wts and its effector Yki in the Hippo signaling pathway
Title: Drosophila C-terminal Src kinase regulates growth via the Hippo signaling pathway.

A functional link between cell polarity, the JAK/STAT signalling pathway and the Src kinases has been established in a whole organism.
Title: Src kinases mediate the interaction of the apical determinant Bazooka/PAR3 with STAT92E and increase signalling efficiency in Drosophila ectodermal cells.

Results demonstrate that regulation of Src64 by Drosophila C-terminal-Src Kinase (Csk) contributes to the packaging of germline cysts by overlying somatic follicle cells during egg chamber formation.
Title: Csk differentially regulates Src64 during distinct morphological events in Drosophila germ cells.

Drosophila C-terminal Src kinase negatively regulates organ growth and cell proliferation through inhibition of the Src, Jun N-terminal kinase, and STAT pathways
Title: Drosophila C-terminal Src kinase negatively regulates organ growth and cell proliferation through inhibition of the Src, Jun N-terminal kinase, and STAT pathways.

The following Csk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Csk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04554	NM_169416.2 Latest version!	Drosophila melanogaster C-terminal Src kinase (Csk), transcript variant K, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa04554	NM_176461.1 Latest version!	Drosophila melanogaster C-terminal Src kinase (Csk), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa04554	NM_169414.2 Latest version!	Drosophila melanogaster C-terminal Src kinase (Csk), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa04554	NM_169415.2 Latest version!	Drosophila melanogaster C-terminal Src kinase (Csk), transcript variant J, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa04555	NM_169418.3 Latest version!	Drosophila melanogaster C-terminal Src kinase (Csk), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa04555	NM_001275547.1 Latest version!	Drosophila melanogaster C-terminal Src kinase (Csk), transcript variant L, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa04556	NM_141840.1 Latest version!	Drosophila melanogaster C-terminal Src kinase (Csk), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Csk ( NM_169416.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731609.1 Drosophila melanogaster C-terminal Src kinase (Csk), transcript variant K, mRNA.

Price & Availability↑

CloneID	OFa04554
Clone ID Related Accession (Same CDS sequence)	NM_169416.2 , NM_176461.1 , NM_169414.2 , NM_169415.2
Accession Version	NM_169416.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	C-terminal Src kinase, isoform K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Feb 27, 2003 this sequence version replaced NM_169416.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACAGCC ACGCGACTGC CACGCAGAAC GGACAAACCA CGGTCGTCGG ACACCATCGC 
GGATTCTCGC AGCCCTCGGT GACGGTGGCC GCTGCAGCGG CAACGGGCAG CACGGCTGGT 
GATCCATGGT TCCTGCAGAC CACCGGTCAC CAGATGCCAC CCACTGCACC GTTGCGGCAC 
AACAAGCGTC CTGCCCCCCA GCCGAATGCG GTTTTGGGAG CGGGTTCCTC GGGAACAGCG 
CCTGGATCGG CGGTTGGCGC CGTACTGCTC CACCAACAGC AGCTCAGCCA GTCGCAGCCG 
CACATTGTGC CGCCCAGCAT ACCGCCGCAC CACCACCACC ATCGCGAGAA CAATGTGCAC 
CCCATCCAGG CGGGCGCGGC AGCCGCATTG CATTTGTCAT CGCATGCGCT TAATAGTCAT 
AATTTAATCA GCAGCGCCAG CAATCATTCG CCCAAGTCCC CCAGCCAGAC GCAGCAGCAG 
CAACAGACGA CGAACCAGCA GCAACAACAG CAGCAGCAGC AGGCGTCCCA CAGCATACTG 
TCCACGTTTG CGCCCGCCCC CACTGTCGCC GCCGTTCAGC CGGCGGGATC GCAGTCCGTT 
GCCGGTACCG CTGCCGCAGT GGCCGCCGTT GCCCAGCAGC AGCAACAGCA GCATCTTCAG 
CTGCTGGCCA ACTGTCCGCC CGGTGGAGCG GGTCTAATGT CCTCCTCCCT GAATGCAGCC 
CAAATGGGCA TGCATGGCGC CGAGCGAGCG CTGCCGCCAA AGAGTCTGGC GCTGAGTGGC 
AGCCAGCACG GCAGCAATAT GCTACTGCAC ACAGCACCGC AACCGCCACA GGCATCCCAG 
CCAGCGGCAG TCGTCGGCGT CAGTGGTAGC GGATCGGCGG GTATGTCCAC CTCGCTGCAC 
AGCTTCGATA TTAATGGAGT CACGGCAGGC AGCGGGGTGA ATGCGAGCAG CGCGTGGTCA 
AGCGGATCCA CCACTGCCAT GAACCATGCT TCCCTCTCGC CCACAGCGTT GGCACCGCAA 
CAAGGTCGGT CCAGGTGCGA GGTCAAACTG AACGCCATGC CATGGTTCCA TGGCAGCATA 
ACGCGGGATG AGGCGGAGCA TCTGCTGCAG CCGCGTGAGG ATGGTTTGTT CCTGGTTCGC 
GAGTCCACAA ACTTCCCCGG CGACTACACG CTCTGCGTTT GTTTCCAGTC CAAGGTGGAG 
CACTACCGGG TCAAGTACCT AGAGAACAAG CTGACCATCG ACGACGAGGA ATACTTTGAG 
AATTTGGGCC AGCTAGTGGC GCACTACGAG GCGGATGCGG ATGGACTGTG CACCCAGCTG 
ATTAAATGCT TGCCAAAGCT GGGCAAACAA GAGTTTTGCA TTAACTCCAA AGATTTCGTG 
GACAAGGGCT GGGTGATCCC TGAAGCGGAG CTGCAGCTGC GCGAGAGCAT CGGCAAGGGC 
GAGTTCGGTG ACGTGATGCT GGGCATATTA CGCAACGAGA AGGTGGCCGT CAAGATGCTG 
AAGGACGAGG GCGCCGTGCA GAAGTTTCTG GCTGAGGCCT CGGTCATGAC CACGCTGGAG 
CACGACAATT TGGTAAAGTT TATCGGCCTT GTCTTCACCA GCAAGCATCT GTATCTGGTT 
ACGGAATATA TGAGTAAGGG ATCACTGGTT GATTACCTGC GATCGCGAGG ACGACAGCAC 
ATAACCAAAA AGGATCAAAT CATTTTTGCC TACGACACTG CCTCTGGTAT GGAGTATCTG 
GAAGCCAAAA AGGTTGTCCA TCGCGATCTG GCAGCTCGCA ATGTCCTTAT ATCAGAGGAT 
TGCGTGGCTA AAGTATCGGA TTTCGGTTTG GCACGCGAGG AATGCTACAA TCTTGACGTT 
GGCAAGCTGC CCATAAAATG GACGGCTCCC GAGGCACTTA AAAATGGGCG TTTTTCCAAC 
AAATCAGACA TGTGGAGCTT TGGCATACTG CTATGGGAAA TCTACTCCTT TGGACGCGTG 
CCCTATCCCA GAATTCCATT AGCCGATGTG GTAAAGCACG TTGAGGTGGG CTACAAAATG 
GAAGCGCCCG AGGGCTGTCC ACCGGAGATT TACGAAATGA TGCGGCAGGC CTGGGACCTA 
AATCCCGCCA AGCGACCCAC ATTCGCGGAG CTCAAGGTCA AACTGCAGCT ACTGAACAAT 
GCCACGGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731609.1
CDS	459..2630
Translation	MNSHATATQNGQTTVVGHHRGFSQPSVTVAAAAATGSTAGDPWFLQTTGHQMPPTAPLRHNKRPAPQPNAVLGAGSSGTAPGSAVGAVLLHQQQLSQSQPHIVPPSIPPHHHHHRENNVHPIQAGAAAALHLSSHALNSHNLISSASNHSPKSPSQTQQQQQTTNQQQQQQQQQASHSILSTFAPAPTVAAVQPAGSQSVAGTAAAVAAVAQQQQQQHLQLLANCPPGGAGLMSSSLNAAQMGMHGAERALPPKSLALSGSQHGSNMLLHTAPQPPQASQPAAVVGVSGSGSAGMSTSLHSFDINGVTAGSGVNASSAWSSGSTTAMNHASLSPTALAPQQGRSRCEVKLNAMPWFHGSITRDEAEHLLQPREDGLFLVRESTNFPGDYTLCVCFQSKVEHYRVKYLENKLTIDDEEYFENLGQLVAHYEADADGLCTQLIKCLPKLGKQEFCINSKDFVDKGWVIPEAELQLRESIGKGEFGDVMLGILRNEKVAVKMLKDEGAVQKFLAEASVMTTLEHDNLVKFIGLVFTSKHLYLVTEYMSKGSLVDYLRSRGRQHITKKDQIIFAYDTASGMEYLEAKKVVHRDLAARNVLISEDCVAKVSDFGLAREECYNLDVGKLPIKWTAPEALKNGRFSNKSDMWSFGILLWEIYSFGRVPYPRIPLADVVKHVEVGYKMEAPEGCPPEIYEMMRQAWDLNPAKRPTFAELKVKLQLLNNATA

Target ORF information:

RefSeq Version	NM_169416.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster C-terminal Src kinase (Csk), transcript variant K, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169416.2

ORF Insert Sequence:

ATGAACAGCC ACGCGACTGC CACGCAGAAC GGACAAACCA CGGTCGTCGG ACACCATCGC 
GGATTCTCGC AGCCCTCGGT GACGGTGGCC GCTGCAGCGG CAACGGGCAG CACGGCTGGT 
GATCCATGGT TCCTGCAGAC CACCGGTCAC CAGATGCCAC CCACTGCACC GTTGCGGCAC 
AACAAGCGTC CTGCCCCCCA GCCGAATGCG GTTTTGGGAG CGGGTTCCTC GGGAACAGCG 
CCTGGATCGG CGGTTGGCGC CGTACTGCTC CACCAACAGC AGCTCAGCCA GTCGCAGCCG 
CACATTGTGC CGCCCAGCAT ACCGCCGCAC CACCACCACC ATCGCGAGAA CAATGTGCAC 
CCCATCCAGG CGGGCGCGGC AGCCGCATTG CATTTGTCAT CGCATGCGCT TAATAGTCAT 
AATTTAATCA GCAGCGCCAG CAATCATTCG CCCAAGTCCC CCAGCCAGAC GCAGCAGCAG 
CAACAGACGA CGAACCAGCA GCAACAACAG CAGCAGCAGC AGGCGTCCCA CAGCATACTG 
TCCACGTTTG CGCCCGCCCC CACTGTCGCC GCCGTTCAGC CGGCGGGATC GCAGTCCGTT 
GCCGGTACCG CTGCCGCAGT GGCCGCCGTT GCCCAGCAGC AGCAACAGCA GCATCTTCAG 
CTGCTGGCCA ACTGTCCGCC CGGTGGAGCG GGTCTAATGT CCTCCTCCCT GAATGCAGCC 
CAAATGGGCA TGCATGGCGC CGAGCGAGCG CTGCCGCCAA AGAGTCTGGC GCTGAGTGGC 
AGCCAGCACG GCAGCAATAT GCTACTGCAC ACAGCACCGC AACCGCCACA GGCATCCCAG 
CCAGCGGCAG TCGTCGGCGT CAGTGGTAGC GGATCGGCGG GTATGTCCAC CTCGCTGCAC 
AGCTTCGATA TTAATGGAGT CACGGCAGGC AGCGGGGTGA ATGCGAGCAG CGCGTGGTCA 
AGCGGATCCA CCACTGCCAT GAACCATGCT TCCCTCTCGC CCACAGCGTT GGCACCGCAA 
CAAGGTCGGT CCAGGTGCGA GGTCAAACTG AACGCCATGC CATGGTTCCA TGGCAGCATA 
ACGCGGGATG AGGCGGAGCA TCTGCTGCAG CCGCGTGAGG ATGGTTTGTT CCTGGTTCGC 
GAGTCCACAA ACTTCCCCGG CGACTACACG CTCTGCGTTT GTTTCCAGTC CAAGGTGGAG 
CACTACCGGG TCAAGTACCT AGAGAACAAG CTGACCATCG ACGACGAGGA ATACTTTGAG 
AATTTGGGCC AGCTAGTGGC GCACTACGAG GCGGATGCGG ATGGACTGTG CACCCAGCTG 
ATTAAATGCT TGCCAAAGCT GGGCAAACAA GAGTTTTGCA TTAACTCCAA AGATTTCGTG 
GACAAGGGCT GGGTGATCCC TGAAGCGGAG CTGCAGCTGC GCGAGAGCAT CGGCAAGGGC 
GAGTTCGGTG ACGTGATGCT GGGCATATTA CGCAACGAGA AGGTGGCCGT CAAGATGCTG 
AAGGACGAGG GCGCCGTGCA GAAGTTTCTG GCTGAGGCCT CGGTCATGAC CACGCTGGAG 
CACGACAATT TGGTAAAGTT TATCGGCCTT GTCTTCACCA GCAAGCATCT GTATCTGGTT 
ACGGAATATA TGAGTAAGGG ATCACTGGTT GATTACCTGC GATCGCGAGG ACGACAGCAC 
ATAACCAAAA AGGATCAAAT CATTTTTGCC TACGACACTG CCTCTGGTAT GGAGTATCTG 
GAAGCCAAAA AGGTTGTCCA TCGCGATCTG GCAGCTCGCA ATGTCCTTAT ATCAGAGGAT 
TGCGTGGCTA AAGTATCGGA TTTCGGTTTG GCACGCGAGG AATGCTACAA TCTTGACGTT 
GGCAAGCTGC CCATAAAATG GACGGCTCCC GAGGCACTTA AAAATGGGCG TTTTTCCAAC 
AAATCAGACA TGTGGAGCTT TGGCATACTG CTATGGGAAA TCTACTCCTT TGGACGCGTG 
CCCTATCCCA GAATTCCATT AGCCGATGTG GTAAAGCACG TTGAGGTGGG CTACAAAATG 
GAAGCGCCCG AGGGCTGTCC ACCGGAGATT TACGAAATGA TGCGGCAGGC CTGGGACCTA 
AATCCCGCCA AGCGACCCAC ATTCGCGGAG CTCAAGGTCA AACTGCAGCT ACTGAACAAT 
GCCACGGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Csk ( NM_176461.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788638.1 Drosophila melanogaster C-terminal Src kinase (Csk), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa04554
Clone ID Related Accession (Same CDS sequence)	NM_169416.2 , NM_176461.1 , NM_169414.2 , NM_169415.2
Accession Version	NM_176461.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	C-terminal Src kinase, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACAGCC ACGCGACTGC CACGCAGAAC GGACAAACCA CGGTCGTCGG ACACCATCGC 
GGATTCTCGC AGCCCTCGGT GACGGTGGCC GCTGCAGCGG CAACGGGCAG CACGGCTGGT 
GATCCATGGT TCCTGCAGAC CACCGGTCAC CAGATGCCAC CCACTGCACC GTTGCGGCAC 
AACAAGCGTC CTGCCCCCCA GCCGAATGCG GTTTTGGGAG CGGGTTCCTC GGGAACAGCG 
CCTGGATCGG CGGTTGGCGC CGTACTGCTC CACCAACAGC AGCTCAGCCA GTCGCAGCCG 
CACATTGTGC CGCCCAGCAT ACCGCCGCAC CACCACCACC ATCGCGAGAA CAATGTGCAC 
CCCATCCAGG CGGGCGCGGC AGCCGCATTG CATTTGTCAT CGCATGCGCT TAATAGTCAT 
AATTTAATCA GCAGCGCCAG CAATCATTCG CCCAAGTCCC CCAGCCAGAC GCAGCAGCAG 
CAACAGACGA CGAACCAGCA GCAACAACAG CAGCAGCAGC AGGCGTCCCA CAGCATACTG 
TCCACGTTTG CGCCCGCCCC CACTGTCGCC GCCGTTCAGC CGGCGGGATC GCAGTCCGTT 
GCCGGTACCG CTGCCGCAGT GGCCGCCGTT GCCCAGCAGC AGCAACAGCA GCATCTTCAG 
CTGCTGGCCA ACTGTCCGCC CGGTGGAGCG GGTCTAATGT CCTCCTCCCT GAATGCAGCC 
CAAATGGGCA TGCATGGCGC CGAGCGAGCG CTGCCGCCAA AGAGTCTGGC GCTGAGTGGC 
AGCCAGCACG GCAGCAATAT GCTACTGCAC ACAGCACCGC AACCGCCACA GGCATCCCAG 
CCAGCGGCAG TCGTCGGCGT CAGTGGTAGC GGATCGGCGG GTATGTCCAC CTCGCTGCAC 
AGCTTCGATA TTAATGGAGT CACGGCAGGC AGCGGGGTGA ATGCGAGCAG CGCGTGGTCA 
AGCGGATCCA CCACTGCCAT GAACCATGCT TCCCTCTCGC CCACAGCGTT GGCACCGCAA 
CAAGGTCGGT CCAGGTGCGA GGTCAAACTG AACGCCATGC CATGGTTCCA TGGCAGCATA 
ACGCGGGATG AGGCGGAGCA TCTGCTGCAG CCGCGTGAGG ATGGTTTGTT CCTGGTTCGC 
GAGTCCACAA ACTTCCCCGG CGACTACACG CTCTGCGTTT GTTTCCAGTC CAAGGTGGAG 
CACTACCGGG TCAAGTACCT AGAGAACAAG CTGACCATCG ACGACGAGGA ATACTTTGAG 
AATTTGGGCC AGCTAGTGGC GCACTACGAG GCGGATGCGG ATGGACTGTG CACCCAGCTG 
ATTAAATGCT TGCCAAAGCT GGGCAAACAA GAGTTTTGCA TTAACTCCAA AGATTTCGTG 
GACAAGGGCT GGGTGATCCC TGAAGCGGAG CTGCAGCTGC GCGAGAGCAT CGGCAAGGGC 
GAGTTCGGTG ACGTGATGCT GGGCATATTA CGCAACGAGA AGGTGGCCGT CAAGATGCTG 
AAGGACGAGG GCGCCGTGCA GAAGTTTCTG GCTGAGGCCT CGGTCATGAC CACGCTGGAG 
CACGACAATT TGGTAAAGTT TATCGGCCTT GTCTTCACCA GCAAGCATCT GTATCTGGTT 
ACGGAATATA TGAGTAAGGG ATCACTGGTT GATTACCTGC GATCGCGAGG ACGACAGCAC 
ATAACCAAAA AGGATCAAAT CATTTTTGCC TACGACACTG CCTCTGGTAT GGAGTATCTG 
GAAGCCAAAA AGGTTGTCCA TCGCGATCTG GCAGCTCGCA ATGTCCTTAT ATCAGAGGAT 
TGCGTGGCTA AAGTATCGGA TTTCGGTTTG GCACGCGAGG AATGCTACAA TCTTGACGTT 
GGCAAGCTGC CCATAAAATG GACGGCTCCC GAGGCACTTA AAAATGGGCG TTTTTCCAAC 
AAATCAGACA TGTGGAGCTT TGGCATACTG CTATGGGAAA TCTACTCCTT TGGACGCGTG 
CCCTATCCCA GAATTCCATT AGCCGATGTG GTAAAGCACG TTGAGGTGGG CTACAAAATG 
GAAGCGCCCG AGGGCTGTCC ACCGGAGATT TACGAAATGA TGCGGCAGGC CTGGGACCTA 
AATCCCGCCA AGCGACCCAC ATTCGCGGAG CTCAAGGTCA AACTGCAGCT ACTGAACAAT 
GCCACGGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788638.1
CDS	233..2404
Translation	MNSHATATQNGQTTVVGHHRGFSQPSVTVAAAAATGSTAGDPWFLQTTGHQMPPTAPLRHNKRPAPQPNAVLGAGSSGTAPGSAVGAVLLHQQQLSQSQPHIVPPSIPPHHHHHRENNVHPIQAGAAAALHLSSHALNSHNLISSASNHSPKSPSQTQQQQQTTNQQQQQQQQQASHSILSTFAPAPTVAAVQPAGSQSVAGTAAAVAAVAQQQQQQHLQLLANCPPGGAGLMSSSLNAAQMGMHGAERALPPKSLALSGSQHGSNMLLHTAPQPPQASQPAAVVGVSGSGSAGMSTSLHSFDINGVTAGSGVNASSAWSSGSTTAMNHASLSPTALAPQQGRSRCEVKLNAMPWFHGSITRDEAEHLLQPREDGLFLVRESTNFPGDYTLCVCFQSKVEHYRVKYLENKLTIDDEEYFENLGQLVAHYEADADGLCTQLIKCLPKLGKQEFCINSKDFVDKGWVIPEAELQLRESIGKGEFGDVMLGILRNEKVAVKMLKDEGAVQKFLAEASVMTTLEHDNLVKFIGLVFTSKHLYLVTEYMSKGSLVDYLRSRGRQHITKKDQIIFAYDTASGMEYLEAKKVVHRDLAARNVLISEDCVAKVSDFGLAREECYNLDVGKLPIKWTAPEALKNGRFSNKSDMWSFGILLWEIYSFGRVPYPRIPLADVVKHVEVGYKMEAPEGCPPEIYEMMRQAWDLNPAKRPTFAELKVKLQLLNNATA

Target ORF information:

RefSeq Version	NM_176461.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster C-terminal Src kinase (Csk), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176461.1

ORF Insert Sequence:

ATGAACAGCC ACGCGACTGC CACGCAGAAC GGACAAACCA CGGTCGTCGG ACACCATCGC 
GGATTCTCGC AGCCCTCGGT GACGGTGGCC GCTGCAGCGG CAACGGGCAG CACGGCTGGT 
GATCCATGGT TCCTGCAGAC CACCGGTCAC CAGATGCCAC CCACTGCACC GTTGCGGCAC 
AACAAGCGTC CTGCCCCCCA GCCGAATGCG GTTTTGGGAG CGGGTTCCTC GGGAACAGCG 
CCTGGATCGG CGGTTGGCGC CGTACTGCTC CACCAACAGC AGCTCAGCCA GTCGCAGCCG 
CACATTGTGC CGCCCAGCAT ACCGCCGCAC CACCACCACC ATCGCGAGAA CAATGTGCAC 
CCCATCCAGG CGGGCGCGGC AGCCGCATTG CATTTGTCAT CGCATGCGCT TAATAGTCAT 
AATTTAATCA GCAGCGCCAG CAATCATTCG CCCAAGTCCC CCAGCCAGAC GCAGCAGCAG 
CAACAGACGA CGAACCAGCA GCAACAACAG CAGCAGCAGC AGGCGTCCCA CAGCATACTG 
TCCACGTTTG CGCCCGCCCC CACTGTCGCC GCCGTTCAGC CGGCGGGATC GCAGTCCGTT 
GCCGGTACCG CTGCCGCAGT GGCCGCCGTT GCCCAGCAGC AGCAACAGCA GCATCTTCAG 
CTGCTGGCCA ACTGTCCGCC CGGTGGAGCG GGTCTAATGT CCTCCTCCCT GAATGCAGCC 
CAAATGGGCA TGCATGGCGC CGAGCGAGCG CTGCCGCCAA AGAGTCTGGC GCTGAGTGGC 
AGCCAGCACG GCAGCAATAT GCTACTGCAC ACAGCACCGC AACCGCCACA GGCATCCCAG 
CCAGCGGCAG TCGTCGGCGT CAGTGGTAGC GGATCGGCGG GTATGTCCAC CTCGCTGCAC 
AGCTTCGATA TTAATGGAGT CACGGCAGGC AGCGGGGTGA ATGCGAGCAG CGCGTGGTCA 
AGCGGATCCA CCACTGCCAT GAACCATGCT TCCCTCTCGC CCACAGCGTT GGCACCGCAA 
CAAGGTCGGT CCAGGTGCGA GGTCAAACTG AACGCCATGC CATGGTTCCA TGGCAGCATA 
ACGCGGGATG AGGCGGAGCA TCTGCTGCAG CCGCGTGAGG ATGGTTTGTT CCTGGTTCGC 
GAGTCCACAA ACTTCCCCGG CGACTACACG CTCTGCGTTT GTTTCCAGTC CAAGGTGGAG 
CACTACCGGG TCAAGTACCT AGAGAACAAG CTGACCATCG ACGACGAGGA ATACTTTGAG 
AATTTGGGCC AGCTAGTGGC GCACTACGAG GCGGATGCGG ATGGACTGTG CACCCAGCTG 
ATTAAATGCT TGCCAAAGCT GGGCAAACAA GAGTTTTGCA TTAACTCCAA AGATTTCGTG 
GACAAGGGCT GGGTGATCCC TGAAGCGGAG CTGCAGCTGC GCGAGAGCAT CGGCAAGGGC 
GAGTTCGGTG ACGTGATGCT GGGCATATTA CGCAACGAGA AGGTGGCCGT CAAGATGCTG 
AAGGACGAGG GCGCCGTGCA GAAGTTTCTG GCTGAGGCCT CGGTCATGAC CACGCTGGAG 
CACGACAATT TGGTAAAGTT TATCGGCCTT GTCTTCACCA GCAAGCATCT GTATCTGGTT 
ACGGAATATA TGAGTAAGGG ATCACTGGTT GATTACCTGC GATCGCGAGG ACGACAGCAC 
ATAACCAAAA AGGATCAAAT CATTTTTGCC TACGACACTG CCTCTGGTAT GGAGTATCTG 
GAAGCCAAAA AGGTTGTCCA TCGCGATCTG GCAGCTCGCA ATGTCCTTAT ATCAGAGGAT 
TGCGTGGCTA AAGTATCGGA TTTCGGTTTG GCACGCGAGG AATGCTACAA TCTTGACGTT 
GGCAAGCTGC CCATAAAATG GACGGCTCCC GAGGCACTTA AAAATGGGCG TTTTTCCAAC 
AAATCAGACA TGTGGAGCTT TGGCATACTG CTATGGGAAA TCTACTCCTT TGGACGCGTG 
CCCTATCCCA GAATTCCATT AGCCGATGTG GTAAAGCACG TTGAGGTGGG CTACAAAATG 
GAAGCGCCCG AGGGCTGTCC ACCGGAGATT TACGAAATGA TGCGGCAGGC CTGGGACCTA 
AATCCCGCCA AGCGACCCAC ATTCGCGGAG CTCAAGGTCA AACTGCAGCT ACTGAACAAT 
GCCACGGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Csk ( NM_169414.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731607.1 Drosophila melanogaster C-terminal Src kinase (Csk), transcript variant I, mRNA.

Price & Availability↑

CloneID	OFa04554
Clone ID Related Accession (Same CDS sequence)	NM_169416.2 , NM_176461.1 , NM_169414.2 , NM_169415.2
Accession Version	NM_169414.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	C-terminal Src kinase, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169414.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACAGCC ACGCGACTGC CACGCAGAAC GGACAAACCA CGGTCGTCGG ACACCATCGC 
GGATTCTCGC AGCCCTCGGT GACGGTGGCC GCTGCAGCGG CAACGGGCAG CACGGCTGGT 
GATCCATGGT TCCTGCAGAC CACCGGTCAC CAGATGCCAC CCACTGCACC GTTGCGGCAC 
AACAAGCGTC CTGCCCCCCA GCCGAATGCG GTTTTGGGAG CGGGTTCCTC GGGAACAGCG 
CCTGGATCGG CGGTTGGCGC CGTACTGCTC CACCAACAGC AGCTCAGCCA GTCGCAGCCG 
CACATTGTGC CGCCCAGCAT ACCGCCGCAC CACCACCACC ATCGCGAGAA CAATGTGCAC 
CCCATCCAGG CGGGCGCGGC AGCCGCATTG CATTTGTCAT CGCATGCGCT TAATAGTCAT 
AATTTAATCA GCAGCGCCAG CAATCATTCG CCCAAGTCCC CCAGCCAGAC GCAGCAGCAG 
CAACAGACGA CGAACCAGCA GCAACAACAG CAGCAGCAGC AGGCGTCCCA CAGCATACTG 
TCCACGTTTG CGCCCGCCCC CACTGTCGCC GCCGTTCAGC CGGCGGGATC GCAGTCCGTT 
GCCGGTACCG CTGCCGCAGT GGCCGCCGTT GCCCAGCAGC AGCAACAGCA GCATCTTCAG 
CTGCTGGCCA ACTGTCCGCC CGGTGGAGCG GGTCTAATGT CCTCCTCCCT GAATGCAGCC 
CAAATGGGCA TGCATGGCGC CGAGCGAGCG CTGCCGCCAA AGAGTCTGGC GCTGAGTGGC 
AGCCAGCACG GCAGCAATAT GCTACTGCAC ACAGCACCGC AACCGCCACA GGCATCCCAG 
CCAGCGGCAG TCGTCGGCGT CAGTGGTAGC GGATCGGCGG GTATGTCCAC CTCGCTGCAC 
AGCTTCGATA TTAATGGAGT CACGGCAGGC AGCGGGGTGA ATGCGAGCAG CGCGTGGTCA 
AGCGGATCCA CCACTGCCAT GAACCATGCT TCCCTCTCGC CCACAGCGTT GGCACCGCAA 
CAAGGTCGGT CCAGGTGCGA GGTCAAACTG AACGCCATGC CATGGTTCCA TGGCAGCATA 
ACGCGGGATG AGGCGGAGCA TCTGCTGCAG CCGCGTGAGG ATGGTTTGTT CCTGGTTCGC 
GAGTCCACAA ACTTCCCCGG CGACTACACG CTCTGCGTTT GTTTCCAGTC CAAGGTGGAG 
CACTACCGGG TCAAGTACCT AGAGAACAAG CTGACCATCG ACGACGAGGA ATACTTTGAG 
AATTTGGGCC AGCTAGTGGC GCACTACGAG GCGGATGCGG ATGGACTGTG CACCCAGCTG 
ATTAAATGCT TGCCAAAGCT GGGCAAACAA GAGTTTTGCA TTAACTCCAA AGATTTCGTG 
GACAAGGGCT GGGTGATCCC TGAAGCGGAG CTGCAGCTGC GCGAGAGCAT CGGCAAGGGC 
GAGTTCGGTG ACGTGATGCT GGGCATATTA CGCAACGAGA AGGTGGCCGT CAAGATGCTG 
AAGGACGAGG GCGCCGTGCA GAAGTTTCTG GCTGAGGCCT CGGTCATGAC CACGCTGGAG 
CACGACAATT TGGTAAAGTT TATCGGCCTT GTCTTCACCA GCAAGCATCT GTATCTGGTT 
ACGGAATATA TGAGTAAGGG ATCACTGGTT GATTACCTGC GATCGCGAGG ACGACAGCAC 
ATAACCAAAA AGGATCAAAT CATTTTTGCC TACGACACTG CCTCTGGTAT GGAGTATCTG 
GAAGCCAAAA AGGTTGTCCA TCGCGATCTG GCAGCTCGCA ATGTCCTTAT ATCAGAGGAT 
TGCGTGGCTA AAGTATCGGA TTTCGGTTTG GCACGCGAGG AATGCTACAA TCTTGACGTT 
GGCAAGCTGC CCATAAAATG GACGGCTCCC GAGGCACTTA AAAATGGGCG TTTTTCCAAC 
AAATCAGACA TGTGGAGCTT TGGCATACTG CTATGGGAAA TCTACTCCTT TGGACGCGTG 
CCCTATCCCA GAATTCCATT AGCCGATGTG GTAAAGCACG TTGAGGTGGG CTACAAAATG 
GAAGCGCCCG AGGGCTGTCC ACCGGAGATT TACGAAATGA TGCGGCAGGC CTGGGACCTA 
AATCCCGCCA AGCGACCCAC ATTCGCGGAG CTCAAGGTCA AACTGCAGCT ACTGAACAAT 
GCCACGGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731607.1
CDS	811..2982
Translation	MNSHATATQNGQTTVVGHHRGFSQPSVTVAAAAATGSTAGDPWFLQTTGHQMPPTAPLRHNKRPAPQPNAVLGAGSSGTAPGSAVGAVLLHQQQLSQSQPHIVPPSIPPHHHHHRENNVHPIQAGAAAALHLSSHALNSHNLISSASNHSPKSPSQTQQQQQTTNQQQQQQQQQASHSILSTFAPAPTVAAVQPAGSQSVAGTAAAVAAVAQQQQQQHLQLLANCPPGGAGLMSSSLNAAQMGMHGAERALPPKSLALSGSQHGSNMLLHTAPQPPQASQPAAVVGVSGSGSAGMSTSLHSFDINGVTAGSGVNASSAWSSGSTTAMNHASLSPTALAPQQGRSRCEVKLNAMPWFHGSITRDEAEHLLQPREDGLFLVRESTNFPGDYTLCVCFQSKVEHYRVKYLENKLTIDDEEYFENLGQLVAHYEADADGLCTQLIKCLPKLGKQEFCINSKDFVDKGWVIPEAELQLRESIGKGEFGDVMLGILRNEKVAVKMLKDEGAVQKFLAEASVMTTLEHDNLVKFIGLVFTSKHLYLVTEYMSKGSLVDYLRSRGRQHITKKDQIIFAYDTASGMEYLEAKKVVHRDLAARNVLISEDCVAKVSDFGLAREECYNLDVGKLPIKWTAPEALKNGRFSNKSDMWSFGILLWEIYSFGRVPYPRIPLADVVKHVEVGYKMEAPEGCPPEIYEMMRQAWDLNPAKRPTFAELKVKLQLLNNATA

Target ORF information:

RefSeq Version	NM_169414.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster C-terminal Src kinase (Csk), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169414.2

ORF Insert Sequence:

ATGAACAGCC ACGCGACTGC CACGCAGAAC GGACAAACCA CGGTCGTCGG ACACCATCGC 
GGATTCTCGC AGCCCTCGGT GACGGTGGCC GCTGCAGCGG CAACGGGCAG CACGGCTGGT 
GATCCATGGT TCCTGCAGAC CACCGGTCAC CAGATGCCAC CCACTGCACC GTTGCGGCAC 
AACAAGCGTC CTGCCCCCCA GCCGAATGCG GTTTTGGGAG CGGGTTCCTC GGGAACAGCG 
CCTGGATCGG CGGTTGGCGC CGTACTGCTC CACCAACAGC AGCTCAGCCA GTCGCAGCCG 
CACATTGTGC CGCCCAGCAT ACCGCCGCAC CACCACCACC ATCGCGAGAA CAATGTGCAC 
CCCATCCAGG CGGGCGCGGC AGCCGCATTG CATTTGTCAT CGCATGCGCT TAATAGTCAT 
AATTTAATCA GCAGCGCCAG CAATCATTCG CCCAAGTCCC CCAGCCAGAC GCAGCAGCAG 
CAACAGACGA CGAACCAGCA GCAACAACAG CAGCAGCAGC AGGCGTCCCA CAGCATACTG 
TCCACGTTTG CGCCCGCCCC CACTGTCGCC GCCGTTCAGC CGGCGGGATC GCAGTCCGTT 
GCCGGTACCG CTGCCGCAGT GGCCGCCGTT GCCCAGCAGC AGCAACAGCA GCATCTTCAG 
CTGCTGGCCA ACTGTCCGCC CGGTGGAGCG GGTCTAATGT CCTCCTCCCT GAATGCAGCC 
CAAATGGGCA TGCATGGCGC CGAGCGAGCG CTGCCGCCAA AGAGTCTGGC GCTGAGTGGC 
AGCCAGCACG GCAGCAATAT GCTACTGCAC ACAGCACCGC AACCGCCACA GGCATCCCAG 
CCAGCGGCAG TCGTCGGCGT CAGTGGTAGC GGATCGGCGG GTATGTCCAC CTCGCTGCAC 
AGCTTCGATA TTAATGGAGT CACGGCAGGC AGCGGGGTGA ATGCGAGCAG CGCGTGGTCA 
AGCGGATCCA CCACTGCCAT GAACCATGCT TCCCTCTCGC CCACAGCGTT GGCACCGCAA 
CAAGGTCGGT CCAGGTGCGA GGTCAAACTG AACGCCATGC CATGGTTCCA TGGCAGCATA 
ACGCGGGATG AGGCGGAGCA TCTGCTGCAG CCGCGTGAGG ATGGTTTGTT CCTGGTTCGC 
GAGTCCACAA ACTTCCCCGG CGACTACACG CTCTGCGTTT GTTTCCAGTC CAAGGTGGAG 
CACTACCGGG TCAAGTACCT AGAGAACAAG CTGACCATCG ACGACGAGGA ATACTTTGAG 
AATTTGGGCC AGCTAGTGGC GCACTACGAG GCGGATGCGG ATGGACTGTG CACCCAGCTG 
ATTAAATGCT TGCCAAAGCT GGGCAAACAA GAGTTTTGCA TTAACTCCAA AGATTTCGTG 
GACAAGGGCT GGGTGATCCC TGAAGCGGAG CTGCAGCTGC GCGAGAGCAT CGGCAAGGGC 
GAGTTCGGTG ACGTGATGCT GGGCATATTA CGCAACGAGA AGGTGGCCGT CAAGATGCTG 
AAGGACGAGG GCGCCGTGCA GAAGTTTCTG GCTGAGGCCT CGGTCATGAC CACGCTGGAG 
CACGACAATT TGGTAAAGTT TATCGGCCTT GTCTTCACCA GCAAGCATCT GTATCTGGTT 
ACGGAATATA TGAGTAAGGG ATCACTGGTT GATTACCTGC GATCGCGAGG ACGACAGCAC 
ATAACCAAAA AGGATCAAAT CATTTTTGCC TACGACACTG CCTCTGGTAT GGAGTATCTG 
GAAGCCAAAA AGGTTGTCCA TCGCGATCTG GCAGCTCGCA ATGTCCTTAT ATCAGAGGAT 
TGCGTGGCTA AAGTATCGGA TTTCGGTTTG GCACGCGAGG AATGCTACAA TCTTGACGTT 
GGCAAGCTGC CCATAAAATG GACGGCTCCC GAGGCACTTA AAAATGGGCG TTTTTCCAAC 
AAATCAGACA TGTGGAGCTT TGGCATACTG CTATGGGAAA TCTACTCCTT TGGACGCGTG 
CCCTATCCCA GAATTCCATT AGCCGATGTG GTAAAGCACG TTGAGGTGGG CTACAAAATG 
GAAGCGCCCG AGGGCTGTCC ACCGGAGATT TACGAAATGA TGCGGCAGGC CTGGGACCTA 
AATCCCGCCA AGCGACCCAC ATTCGCGGAG CTCAAGGTCA AACTGCAGCT ACTGAACAAT 
GCCACGGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Csk ( NM_169415.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731608.1 Drosophila melanogaster C-terminal Src kinase (Csk), transcript variant J, mRNA.

Price & Availability↑

CloneID	OFa04554
Clone ID Related Accession (Same CDS sequence)	NM_169416.2 , NM_176461.1 , NM_169414.2 , NM_169415.2
Accession Version	NM_169415.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	C-terminal Src kinase, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Feb 27, 2003 this sequence version replaced NM_169415.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACAGCC ACGCGACTGC CACGCAGAAC GGACAAACCA CGGTCGTCGG ACACCATCGC 
GGATTCTCGC AGCCCTCGGT GACGGTGGCC GCTGCAGCGG CAACGGGCAG CACGGCTGGT 
GATCCATGGT TCCTGCAGAC CACCGGTCAC CAGATGCCAC CCACTGCACC GTTGCGGCAC 
AACAAGCGTC CTGCCCCCCA GCCGAATGCG GTTTTGGGAG CGGGTTCCTC GGGAACAGCG 
CCTGGATCGG CGGTTGGCGC CGTACTGCTC CACCAACAGC AGCTCAGCCA GTCGCAGCCG 
CACATTGTGC CGCCCAGCAT ACCGCCGCAC CACCACCACC ATCGCGAGAA CAATGTGCAC 
CCCATCCAGG CGGGCGCGGC AGCCGCATTG CATTTGTCAT CGCATGCGCT TAATAGTCAT 
AATTTAATCA GCAGCGCCAG CAATCATTCG CCCAAGTCCC CCAGCCAGAC GCAGCAGCAG 
CAACAGACGA CGAACCAGCA GCAACAACAG CAGCAGCAGC AGGCGTCCCA CAGCATACTG 
TCCACGTTTG CGCCCGCCCC CACTGTCGCC GCCGTTCAGC CGGCGGGATC GCAGTCCGTT 
GCCGGTACCG CTGCCGCAGT GGCCGCCGTT GCCCAGCAGC AGCAACAGCA GCATCTTCAG 
CTGCTGGCCA ACTGTCCGCC CGGTGGAGCG GGTCTAATGT CCTCCTCCCT GAATGCAGCC 
CAAATGGGCA TGCATGGCGC CGAGCGAGCG CTGCCGCCAA AGAGTCTGGC GCTGAGTGGC 
AGCCAGCACG GCAGCAATAT GCTACTGCAC ACAGCACCGC AACCGCCACA GGCATCCCAG 
CCAGCGGCAG TCGTCGGCGT CAGTGGTAGC GGATCGGCGG GTATGTCCAC CTCGCTGCAC 
AGCTTCGATA TTAATGGAGT CACGGCAGGC AGCGGGGTGA ATGCGAGCAG CGCGTGGTCA 
AGCGGATCCA CCACTGCCAT GAACCATGCT TCCCTCTCGC CCACAGCGTT GGCACCGCAA 
CAAGGTCGGT CCAGGTGCGA GGTCAAACTG AACGCCATGC CATGGTTCCA TGGCAGCATA 
ACGCGGGATG AGGCGGAGCA TCTGCTGCAG CCGCGTGAGG ATGGTTTGTT CCTGGTTCGC 
GAGTCCACAA ACTTCCCCGG CGACTACACG CTCTGCGTTT GTTTCCAGTC CAAGGTGGAG 
CACTACCGGG TCAAGTACCT AGAGAACAAG CTGACCATCG ACGACGAGGA ATACTTTGAG 
AATTTGGGCC AGCTAGTGGC GCACTACGAG GCGGATGCGG ATGGACTGTG CACCCAGCTG 
ATTAAATGCT TGCCAAAGCT GGGCAAACAA GAGTTTTGCA TTAACTCCAA AGATTTCGTG 
GACAAGGGCT GGGTGATCCC TGAAGCGGAG CTGCAGCTGC GCGAGAGCAT CGGCAAGGGC 
GAGTTCGGTG ACGTGATGCT GGGCATATTA CGCAACGAGA AGGTGGCCGT CAAGATGCTG 
AAGGACGAGG GCGCCGTGCA GAAGTTTCTG GCTGAGGCCT CGGTCATGAC CACGCTGGAG 
CACGACAATT TGGTAAAGTT TATCGGCCTT GTCTTCACCA GCAAGCATCT GTATCTGGTT 
ACGGAATATA TGAGTAAGGG ATCACTGGTT GATTACCTGC GATCGCGAGG ACGACAGCAC 
ATAACCAAAA AGGATCAAAT CATTTTTGCC TACGACACTG CCTCTGGTAT GGAGTATCTG 
GAAGCCAAAA AGGTTGTCCA TCGCGATCTG GCAGCTCGCA ATGTCCTTAT ATCAGAGGAT 
TGCGTGGCTA AAGTATCGGA TTTCGGTTTG GCACGCGAGG AATGCTACAA TCTTGACGTT 
GGCAAGCTGC CCATAAAATG GACGGCTCCC GAGGCACTTA AAAATGGGCG TTTTTCCAAC 
AAATCAGACA TGTGGAGCTT TGGCATACTG CTATGGGAAA TCTACTCCTT TGGACGCGTG 
CCCTATCCCA GAATTCCATT AGCCGATGTG GTAAAGCACG TTGAGGTGGG CTACAAAATG 
GAAGCGCCCG AGGGCTGTCC ACCGGAGATT TACGAAATGA TGCGGCAGGC CTGGGACCTA 
AATCCCGCCA AGCGACCCAC ATTCGCGGAG CTCAAGGTCA AACTGCAGCT ACTGAACAAT 
GCCACGGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731608.1
CDS	239..2410
Translation	MNSHATATQNGQTTVVGHHRGFSQPSVTVAAAAATGSTAGDPWFLQTTGHQMPPTAPLRHNKRPAPQPNAVLGAGSSGTAPGSAVGAVLLHQQQLSQSQPHIVPPSIPPHHHHHRENNVHPIQAGAAAALHLSSHALNSHNLISSASNHSPKSPSQTQQQQQTTNQQQQQQQQQASHSILSTFAPAPTVAAVQPAGSQSVAGTAAAVAAVAQQQQQQHLQLLANCPPGGAGLMSSSLNAAQMGMHGAERALPPKSLALSGSQHGSNMLLHTAPQPPQASQPAAVVGVSGSGSAGMSTSLHSFDINGVTAGSGVNASSAWSSGSTTAMNHASLSPTALAPQQGRSRCEVKLNAMPWFHGSITRDEAEHLLQPREDGLFLVRESTNFPGDYTLCVCFQSKVEHYRVKYLENKLTIDDEEYFENLGQLVAHYEADADGLCTQLIKCLPKLGKQEFCINSKDFVDKGWVIPEAELQLRESIGKGEFGDVMLGILRNEKVAVKMLKDEGAVQKFLAEASVMTTLEHDNLVKFIGLVFTSKHLYLVTEYMSKGSLVDYLRSRGRQHITKKDQIIFAYDTASGMEYLEAKKVVHRDLAARNVLISEDCVAKVSDFGLAREECYNLDVGKLPIKWTAPEALKNGRFSNKSDMWSFGILLWEIYSFGRVPYPRIPLADVVKHVEVGYKMEAPEGCPPEIYEMMRQAWDLNPAKRPTFAELKVKLQLLNNATA

Target ORF information:

RefSeq Version	NM_169415.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster C-terminal Src kinase (Csk), transcript variant J, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169415.2

ORF Insert Sequence:

ATGAACAGCC ACGCGACTGC CACGCAGAAC GGACAAACCA CGGTCGTCGG ACACCATCGC 
GGATTCTCGC AGCCCTCGGT GACGGTGGCC GCTGCAGCGG CAACGGGCAG CACGGCTGGT 
GATCCATGGT TCCTGCAGAC CACCGGTCAC CAGATGCCAC CCACTGCACC GTTGCGGCAC 
AACAAGCGTC CTGCCCCCCA GCCGAATGCG GTTTTGGGAG CGGGTTCCTC GGGAACAGCG 
CCTGGATCGG CGGTTGGCGC CGTACTGCTC CACCAACAGC AGCTCAGCCA GTCGCAGCCG 
CACATTGTGC CGCCCAGCAT ACCGCCGCAC CACCACCACC ATCGCGAGAA CAATGTGCAC 
CCCATCCAGG CGGGCGCGGC AGCCGCATTG CATTTGTCAT CGCATGCGCT TAATAGTCAT 
AATTTAATCA GCAGCGCCAG CAATCATTCG CCCAAGTCCC CCAGCCAGAC GCAGCAGCAG 
CAACAGACGA CGAACCAGCA GCAACAACAG CAGCAGCAGC AGGCGTCCCA CAGCATACTG 
TCCACGTTTG CGCCCGCCCC CACTGTCGCC GCCGTTCAGC CGGCGGGATC GCAGTCCGTT 
GCCGGTACCG CTGCCGCAGT GGCCGCCGTT GCCCAGCAGC AGCAACAGCA GCATCTTCAG 
CTGCTGGCCA ACTGTCCGCC CGGTGGAGCG GGTCTAATGT CCTCCTCCCT GAATGCAGCC 
CAAATGGGCA TGCATGGCGC CGAGCGAGCG CTGCCGCCAA AGAGTCTGGC GCTGAGTGGC 
AGCCAGCACG GCAGCAATAT GCTACTGCAC ACAGCACCGC AACCGCCACA GGCATCCCAG 
CCAGCGGCAG TCGTCGGCGT CAGTGGTAGC GGATCGGCGG GTATGTCCAC CTCGCTGCAC 
AGCTTCGATA TTAATGGAGT CACGGCAGGC AGCGGGGTGA ATGCGAGCAG CGCGTGGTCA 
AGCGGATCCA CCACTGCCAT GAACCATGCT TCCCTCTCGC CCACAGCGTT GGCACCGCAA 
CAAGGTCGGT CCAGGTGCGA GGTCAAACTG AACGCCATGC CATGGTTCCA TGGCAGCATA 
ACGCGGGATG AGGCGGAGCA TCTGCTGCAG CCGCGTGAGG ATGGTTTGTT CCTGGTTCGC 
GAGTCCACAA ACTTCCCCGG CGACTACACG CTCTGCGTTT GTTTCCAGTC CAAGGTGGAG 
CACTACCGGG TCAAGTACCT AGAGAACAAG CTGACCATCG ACGACGAGGA ATACTTTGAG 
AATTTGGGCC AGCTAGTGGC GCACTACGAG GCGGATGCGG ATGGACTGTG CACCCAGCTG 
ATTAAATGCT TGCCAAAGCT GGGCAAACAA GAGTTTTGCA TTAACTCCAA AGATTTCGTG 
GACAAGGGCT GGGTGATCCC TGAAGCGGAG CTGCAGCTGC GCGAGAGCAT CGGCAAGGGC 
GAGTTCGGTG ACGTGATGCT GGGCATATTA CGCAACGAGA AGGTGGCCGT CAAGATGCTG 
AAGGACGAGG GCGCCGTGCA GAAGTTTCTG GCTGAGGCCT CGGTCATGAC CACGCTGGAG 
CACGACAATT TGGTAAAGTT TATCGGCCTT GTCTTCACCA GCAAGCATCT GTATCTGGTT 
ACGGAATATA TGAGTAAGGG ATCACTGGTT GATTACCTGC GATCGCGAGG ACGACAGCAC 
ATAACCAAAA AGGATCAAAT CATTTTTGCC TACGACACTG CCTCTGGTAT GGAGTATCTG 
GAAGCCAAAA AGGTTGTCCA TCGCGATCTG GCAGCTCGCA ATGTCCTTAT ATCAGAGGAT 
TGCGTGGCTA AAGTATCGGA TTTCGGTTTG GCACGCGAGG AATGCTACAA TCTTGACGTT 
GGCAAGCTGC CCATAAAATG GACGGCTCCC GAGGCACTTA AAAATGGGCG TTTTTCCAAC 
AAATCAGACA TGTGGAGCTT TGGCATACTG CTATGGGAAA TCTACTCCTT TGGACGCGTG 
CCCTATCCCA GAATTCCATT AGCCGATGTG GTAAAGCACG TTGAGGTGGG CTACAAAATG 
GAAGCGCCCG AGGGCTGTCC ACCGGAGATT TACGAAATGA TGCGGCAGGC CTGGGACCTA 
AATCCCGCCA AGCGACCCAC ATTCGCGGAG CTCAAGGTCA AACTGCAGCT ACTGAACAAT 
GCCACGGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Csk ( NM_169418.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731611.2 Drosophila melanogaster C-terminal Src kinase (Csk), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa04555
Clone ID Related Accession (Same CDS sequence)	NM_169418.3 , NM_001275547.1
Accession Version	NM_169418.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	C-terminal Src kinase, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169418.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGACAA CAATTAAGAT AACCTGCGAT GGAGTGTTCA TCCAGTCCAA CTCGTACCTC 
ATGCCCACGC CTTCTCCCAG CGGAGCAGAC GCCACGGTAT CGTCATCCTT GGAACGGGTG 
CTGAAACCCG CGTCCGCATC CGCCTCTCCA TCCAATTCGA ACAACGAAAG TGATAACTCG 
CAGACGAGCA GTGGCAGCCA GACGGCGTCC AGTGCCAGCA AGCGATCCAA TCGATCCGTG 
GGCCGGAGTC ATCGAGGTTC GATTGCGCAG GACACAAGGC GACGCTACTA CTTTGGCAGC 
AAGGACATCT ACTGGTGCAA GCAGAGCAGG GAGGCAGCTG CGGCGGCCAC GCTCTCGGGC 
TCGGGACTAA CGCGTAGTGC CAGCAGTGTC ACCCACAAGG AAGCGTCCAC CCACGCCACC 
CACACTGGTG GTCAGGTAAT CTCCCGTGCG CGTCGCAAGC CCATCCTGCT TTGCAGCAGC 
CTGAGCGCTG CCTATGCTCC TCCTCGTCGG CGTGAGTTTT ACTTCTGCGA CAAGTATGCA 
CCTGGTCCAG CAAGAAGTAC TCCTACTACA ACCACAACAT CCGCAACATC GGCCAAACAC 
GAATCCTCCC CGCCCATCGA CGCACGTCTG CTGAACTTCC TGCGGTGCGC ACATCGAAAG 
TTCCAGGCAT CCAAAGCCAA AACTGAGCTA CAGCAAACCA AAGACAGATT CCTGGGAAAT 
TCCAGAGAGC GGACTAGTAA GAACGGGCAG CAGACGTCCC GCTCGCTGCC ACCCATTGCC 
AGTTCGGAGG AGTGGTTCGA CGAGGAGGCA CAAGCCAGGA AGACTAGCCA CGAGAAGAGC 
CAGAAGCCCA AGCCGAAGCC GGAGGAAAAC CATAAGGAGA AGGAAGCGAT CTCGAGAAAG 
GCGTCCGGCG AGGGTGATCT GATCACCGTT GAGATCAAGC AGGGATTGCT GAGGAAGCAG 
ACGGCAACCA CGACGACCGG CCAGGCTATG AACAGCCACG CGACTGCCAC GCAGAACGGA 
CAAACCACGG TCGTCGGACA CCATCGCGGA TTCTCGCAGC CCTCGGTGAC GGTGGCCGCT 
GCAGCGGCAA CGGGCAGCAC GGCTGGTGAT CCATGGTTCC TGCAGACCAC CGGTCACCAG 
ATGCCACCCA CTGCACCGTT GCGGCACAAC AAGCGTCCTG CCCCCCAGCC GAATGCGGTT 
TTGGGAGCGG GTTCCTCGGG AACAGCGCCT GGATCGGCGG TTGGCGCCGT ACTGCTCCAC 
CAACAGCAGC TCAGCCAGTC GCAGCCGCAC ATTGTGCCGC CCAGCATACC GCCGCACCAC 
CACCACCATC GCGAGAACAA TGTGCACCCC ATCCAGGCGG GCGCGGCAGC CGCATTGCAT 
TTGTCATCGC ATGCGCTTAA TAGTCATAAT TTAATCAGCA GCGCCAGCAA TCATTCGCCC 
AAGTCCCCCA GCCAGACGCA GCAGCAGCAA CAGACGACGA ACCAGCAGCA ACAACAGCAG 
CAGCAGCAGG CGTCCCACAG CATACTGTCC ACGTTTGCGC CCGCCCCCAC TGTCGCCGCC 
GTTCAGCCGG CGGGATCGCA GTCCGTTGCC GGTACCGCTG CCGCAGTGGC CGCCGTTGCC 
CAGCAGCAGC AACAGCAGCA TCTTCAGCTG CTGGCCAACT GTCCGCCCGG TGGAGCGGGT 
CTAATGTCCT CCTCCCTGAA TGCAGCCCAA ATGGGCATGC ATGGCGCCGA GCGAGCGCTG 
CCGCCAAAGA GTCTGGCGCT GAGTGGCAGC CAGCACGGCA GCAATATGCT ACTGCACACA 
GCACCGCAAC CGCCACAGGC ATCCCAGCCA GCGGCAGTCG TCGGCGTCAG TGGTAGCGGA 
TCGGCGGGTA TGTCCACCTC GCTGCACAGC TTCGATATTA ATGGAGTCAC GGCAGGCAGC 
GGGGTGAATG CGAGCAGCGC GTGGTCAAGC GGATCCACCA CTGCCATGAA CCATGCTTCC 
CTCTCGCCCA CAGCGTTGGC ACCGCAACAA GGTCGGTCCA GGTGCGAGGT CAAACTGAAC 
GCCATGCCAT GGTTCCATGG CAGCATAACG CGGGATGAGG CGGAGCATCT GCTGCAGCCG 
CGTGAGGATG GTTTGTTCCT GGTTCGCGAG TCCACAAACT TCCCCGGCGA CTACACGCTC 
TGCGTTTGTT TCCAGTCCAA GGTGGAGCAC TACCGGGTCA AGTACCTAGA GAACAAGCTG 
ACCATCGACG ACGAGGAATA CTTTGAGAAT TTGGGCCAGC TAGTGGCGCA CTACGAGGCG 
GATGCGGATG GACTGTGCAC CCAGCTGATT AAATGCTTGC CAAAGCTGGG CAAACAAGAG 
TTTTGCATTA ACTCCAAAGA TTTCGTGGAC AAGGGCTGGG TGATCCCTGA AGCGGAGCTG 
CAGCTGCGCG AGAGCATCGG CAAGGGCGAG TTCGGTGACG TGATGCTGGG CATATTACGC 
AACGAGAAGG TGGCCGTCAA GATGCTGAAG GACGAGGGCG CCGTGCAGAA GTTTCTGGCT 
GAGGCCTCGG TCATGACCAC GCTGGAGCAC GACAATTTGG TAAAGTTTAT CGGCCTTGTC 
TTCACCAGCA AGCATCTGTA TCTGGTTACG GAATATATGA GTAAGGGATC ACTGGTTGAT 
TACCTGCGAT CGCGAGGACG ACAGCACATA ACCAAAAAGG ATCAAATCAT TTTTGCCTAC 
GACACTGCCT CTGGTATGGA GTATCTGGAA GCCAAAAAGG TTGTCCATCG CGATCTGGCA 
GCTCGCAATG TCCTTATATC AGAGGATTGC GTGGCTAAAG TATCGGATTT CGGTTTGGCA 
CGCGAGGAAT GCTACAATCT TGACGTTGGC AAGCTGCCCA TAAAATGGAC GGCTCCCGAG 
GCACTTAAAA ATGGGCGTTT TTCCAACAAA TCAGACATGT GGAGCTTTGG CATACTGCTA 
TGGGAAATCT ACTCCTTTGG ACGCGTGCCC TATCCCAGAA TTCCATTAGC CGATGTGGTA 
AAGCACGTTG AGGTGGGCTA CAAAATGGAA GCGCCCGAGG GCTGTCCACC GGAGATTTAC 
GAAATGATGC GGCAGGCCTG GGACCTAAAT CCCGCCAAGC GACCCACATT CGCGGAGCTC 
AAGGTCAAAC TGCAGCTACT GAACAATGCC ACGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731611.2
CDS	393..3551
Translation	MVTTIKITCDGVFIQSNSYLMPTPSPSGADATVSSSLERVLKPASASASPSNSNNESDNSQTSSGSQTASSASKRSNRSVGRSHRGSIAQDTRRRYYFGSKDIYWCKQSREAAAAATLSGSGLTRSASSVTHKEASTHATHTGGQVISRARRKPILLCSSLSAAYAPPRRREFYFCDKYAPGPARSTPTTTTTSATSAKHESSPPIDARLLNFLRCAHRKFQASKAKTELQQTKDRFLGNSRERTSKNGQQTSRSLPPIASSEEWFDEEAQARKTSHEKSQKPKPKPEENHKEKEAISRKASGEGDLITVEIKQGLLRKQTATTTTGQAMNSHATATQNGQTTVVGHHRGFSQPSVTVAAAAATGSTAGDPWFLQTTGHQMPPTAPLRHNKRPAPQPNAVLGAGSSGTAPGSAVGAVLLHQQQLSQSQPHIVPPSIPPHHHHHRENNVHPIQAGAAAALHLSSHALNSHNLISSASNHSPKSPSQTQQQQQTTNQQQQQQQQQASHSILSTFAPAPTVAAVQPAGSQSVAGTAAAVAAVAQQQQQQHLQLLANCPPGGAGLMSSSLNAAQMGMHGAERALPPKSLALSGSQHGSNMLLHTAPQPPQASQPAAVVGVSGSGSAGMSTSLHSFDINGVTAGSGVNASSAWSSGSTTAMNHASLSPTALAPQQGRSRCEVKLNAMPWFHGSITRDEAEHLLQPREDGLFLVRESTNFPGDYTLCVCFQSKVEHYRVKYLENKLTIDDEEYFENLGQLVAHYEADADGLCTQLIKCLPKLGKQEFCINSKDFVDKGWVIPEAELQLRESIGKGEFGDVMLGILRNEKVAVKMLKDEGAVQKFLAEASVMTTLEHDNLVKFIGLVFTSKHLYLVTEYMSKGSLVDYLRSRGRQHITKKDQIIFAYDTASGMEYLEAKKVVHRDLAARNVLISEDCVAKVSDFGLAREECYNLDVGKLPIKWTAPEALKNGRFSNKSDMWSFGILLWEIYSFGRVPYPRIPLADVVKHVEVGYKMEAPEGCPPEIYEMMRQAWDLNPAKRPTFAELKVKLQLLNNATA

Target ORF information:

RefSeq Version	NM_169418.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster C-terminal Src kinase (Csk), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169418.3

ORF Insert Sequence:

ATGGTGACAA CAATTAAGAT AACCTGCGAT GGAGTGTTCA TCCAGTCCAA CTCGTACCTC 
ATGCCCACGC CTTCTCCCAG CGGAGCAGAC GCCACGGTAT CGTCATCCTT GGAACGGGTG 
CTGAAACCCG CGTCCGCATC CGCCTCTCCA TCCAATTCGA ACAACGAAAG TGATAACTCG 
CAGACGAGCA GTGGCAGCCA GACGGCGTCC AGTGCCAGCA AGCGATCCAA TCGATCCGTG 
GGCCGGAGTC ATCGAGGTTC GATTGCGCAG GACACAAGGC GACGCTACTA CTTTGGCAGC 
AAGGACATCT ACTGGTGCAA GCAGAGCAGG GAGGCAGCTG CGGCGGCCAC GCTCTCGGGC 
TCGGGACTAA CGCGTAGTGC CAGCAGTGTC ACCCACAAGG AAGCGTCCAC CCACGCCACC 
CACACTGGTG GTCAGGTAAT CTCCCGTGCG CGTCGCAAGC CCATCCTGCT TTGCAGCAGC 
CTGAGCGCTG CCTATGCTCC TCCTCGTCGG CGTGAGTTTT ACTTCTGCGA CAAGTATGCA 
CCTGGTCCAG CAAGAAGTAC TCCTACTACA ACCACAACAT CCGCAACATC GGCCAAACAC 
GAATCCTCCC CGCCCATCGA CGCACGTCTG CTGAACTTCC TGCGGTGCGC ACATCGAAAG 
TTCCAGGCAT CCAAAGCCAA AACTGAGCTA CAGCAAACCA AAGACAGATT CCTGGGAAAT 
TCCAGAGAGC GGACTAGTAA GAACGGGCAG CAGACGTCCC GCTCGCTGCC ACCCATTGCC 
AGTTCGGAGG AGTGGTTCGA CGAGGAGGCA CAAGCCAGGA AGACTAGCCA CGAGAAGAGC 
CAGAAGCCCA AGCCGAAGCC GGAGGAAAAC CATAAGGAGA AGGAAGCGAT CTCGAGAAAG 
GCGTCCGGCG AGGGTGATCT GATCACCGTT GAGATCAAGC AGGGATTGCT GAGGAAGCAG 
ACGGCAACCA CGACGACCGG CCAGGCTATG AACAGCCACG CGACTGCCAC GCAGAACGGA 
CAAACCACGG TCGTCGGACA CCATCGCGGA TTCTCGCAGC CCTCGGTGAC GGTGGCCGCT 
GCAGCGGCAA CGGGCAGCAC GGCTGGTGAT CCATGGTTCC TGCAGACCAC CGGTCACCAG 
ATGCCACCCA CTGCACCGTT GCGGCACAAC AAGCGTCCTG CCCCCCAGCC GAATGCGGTT 
TTGGGAGCGG GTTCCTCGGG AACAGCGCCT GGATCGGCGG TTGGCGCCGT ACTGCTCCAC 
CAACAGCAGC TCAGCCAGTC GCAGCCGCAC ATTGTGCCGC CCAGCATACC GCCGCACCAC 
CACCACCATC GCGAGAACAA TGTGCACCCC ATCCAGGCGG GCGCGGCAGC CGCATTGCAT 
TTGTCATCGC ATGCGCTTAA TAGTCATAAT TTAATCAGCA GCGCCAGCAA TCATTCGCCC 
AAGTCCCCCA GCCAGACGCA GCAGCAGCAA CAGACGACGA ACCAGCAGCA ACAACAGCAG 
CAGCAGCAGG CGTCCCACAG CATACTGTCC ACGTTTGCGC CCGCCCCCAC TGTCGCCGCC 
GTTCAGCCGG CGGGATCGCA GTCCGTTGCC GGTACCGCTG CCGCAGTGGC CGCCGTTGCC 
CAGCAGCAGC AACAGCAGCA TCTTCAGCTG CTGGCCAACT GTCCGCCCGG TGGAGCGGGT 
CTAATGTCCT CCTCCCTGAA TGCAGCCCAA ATGGGCATGC ATGGCGCCGA GCGAGCGCTG 
CCGCCAAAGA GTCTGGCGCT GAGTGGCAGC CAGCACGGCA GCAATATGCT ACTGCACACA 
GCACCGCAAC CGCCACAGGC ATCCCAGCCA GCGGCAGTCG TCGGCGTCAG TGGTAGCGGA 
TCGGCGGGTA TGTCCACCTC GCTGCACAGC TTCGATATTA ATGGAGTCAC GGCAGGCAGC 
GGGGTGAATG CGAGCAGCGC GTGGTCAAGC GGATCCACCA CTGCCATGAA CCATGCTTCC 
CTCTCGCCCA CAGCGTTGGC ACCGCAACAA GGTCGGTCCA GGTGCGAGGT CAAACTGAAC 
GCCATGCCAT GGTTCCATGG CAGCATAACG CGGGATGAGG CGGAGCATCT GCTGCAGCCG 
CGTGAGGATG GTTTGTTCCT GGTTCGCGAG TCCACAAACT TCCCCGGCGA CTACACGCTC 
TGCGTTTGTT TCCAGTCCAA GGTGGAGCAC TACCGGGTCA AGTACCTAGA GAACAAGCTG 
ACCATCGACG ACGAGGAATA CTTTGAGAAT TTGGGCCAGC TAGTGGCGCA CTACGAGGCG 
GATGCGGATG GACTGTGCAC CCAGCTGATT AAATGCTTGC CAAAGCTGGG CAAACAAGAG 
TTTTGCATTA ACTCCAAAGA TTTCGTGGAC AAGGGCTGGG TGATCCCTGA AGCGGAGCTG 
CAGCTGCGCG AGAGCATCGG CAAGGGCGAG TTCGGTGACG TGATGCTGGG CATATTACGC 
AACGAGAAGG TGGCCGTCAA GATGCTGAAG GACGAGGGCG CCGTGCAGAA GTTTCTGGCT 
GAGGCCTCGG TCATGACCAC GCTGGAGCAC GACAATTTGG TAAAGTTTAT CGGCCTTGTC 
TTCACCAGCA AGCATCTGTA TCTGGTTACG GAATATATGA GTAAGGGATC ACTGGTTGAT 
TACCTGCGAT CGCGAGGACG ACAGCACATA ACCAAAAAGG ATCAAATCAT TTTTGCCTAC 
GACACTGCCT CTGGTATGGA GTATCTGGAA GCCAAAAAGG TTGTCCATCG CGATCTGGCA 
GCTCGCAATG TCCTTATATC AGAGGATTGC GTGGCTAAAG TATCGGATTT CGGTTTGGCA 
CGCGAGGAAT GCTACAATCT TGACGTTGGC AAGCTGCCCA TAAAATGGAC GGCTCCCGAG 
GCACTTAAAA ATGGGCGTTT TTCCAACAAA TCAGACATGT GGAGCTTTGG CATACTGCTA 
TGGGAAATCT ACTCCTTTGG ACGCGTGCCC TATCCCAGAA TTCCATTAGC CGATGTGGTA 
AAGCACGTTG AGGTGGGCTA CAAAATGGAA GCGCCCGAGG GCTGTCCACC GGAGATTTAC 
GAAATGATGC GGCAGGCCTG GGACCTAAAT CCCGCCAAGC GACCCACATT CGCGGAGCTC 
AAGGTCAAAC TGCAGCTACT GAACAATGCC ACGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Csk ( NM_001275547.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262476.1 Drosophila melanogaster C-terminal Src kinase (Csk), transcript variant L, mRNA.

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CloneID	OFa04555
Clone ID Related Accession (Same CDS sequence)	NM_169418.3 , NM_001275547.1
Accession Version	NM_001275547.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	C-terminal Src kinase, isoform L
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGACAA CAATTAAGAT AACCTGCGAT GGAGTGTTCA TCCAGTCCAA CTCGTACCTC 
ATGCCCACGC CTTCTCCCAG CGGAGCAGAC GCCACGGTAT CGTCATCCTT GGAACGGGTG 
CTGAAACCCG CGTCCGCATC CGCCTCTCCA TCCAATTCGA ACAACGAAAG TGATAACTCG 
CAGACGAGCA GTGGCAGCCA GACGGCGTCC AGTGCCAGCA AGCGATCCAA TCGATCCGTG 
GGCCGGAGTC ATCGAGGTTC GATTGCGCAG GACACAAGGC GACGCTACTA CTTTGGCAGC 
AAGGACATCT ACTGGTGCAA GCAGAGCAGG GAGGCAGCTG CGGCGGCCAC GCTCTCGGGC 
TCGGGACTAA CGCGTAGTGC CAGCAGTGTC ACCCACAAGG AAGCGTCCAC CCACGCCACC 
CACACTGGTG GTCAGGTAAT CTCCCGTGCG CGTCGCAAGC CCATCCTGCT TTGCAGCAGC 
CTGAGCGCTG CCTATGCTCC TCCTCGTCGG CGTGAGTTTT ACTTCTGCGA CAAGTATGCA 
CCTGGTCCAG CAAGAAGTAC TCCTACTACA ACCACAACAT CCGCAACATC GGCCAAACAC 
GAATCCTCCC CGCCCATCGA CGCACGTCTG CTGAACTTCC TGCGGTGCGC ACATCGAAAG 
TTCCAGGCAT CCAAAGCCAA AACTGAGCTA CAGCAAACCA AAGACAGATT CCTGGGAAAT 
TCCAGAGAGC GGACTAGTAA GAACGGGCAG CAGACGTCCC GCTCGCTGCC ACCCATTGCC 
AGTTCGGAGG AGTGGTTCGA CGAGGAGGCA CAAGCCAGGA AGACTAGCCA CGAGAAGAGC 
CAGAAGCCCA AGCCGAAGCC GGAGGAAAAC CATAAGGAGA AGGAAGCGAT CTCGAGAAAG 
GCGTCCGGCG AGGGTGATCT GATCACCGTT GAGATCAAGC AGGGATTGCT GAGGAAGCAG 
ACGGCAACCA CGACGACCGG CCAGGCTATG AACAGCCACG CGACTGCCAC GCAGAACGGA 
CAAACCACGG TCGTCGGACA CCATCGCGGA TTCTCGCAGC CCTCGGTGAC GGTGGCCGCT 
GCAGCGGCAA CGGGCAGCAC GGCTGGTGAT CCATGGTTCC TGCAGACCAC CGGTCACCAG 
ATGCCACCCA CTGCACCGTT GCGGCACAAC AAGCGTCCTG CCCCCCAGCC GAATGCGGTT 
TTGGGAGCGG GTTCCTCGGG AACAGCGCCT GGATCGGCGG TTGGCGCCGT ACTGCTCCAC 
CAACAGCAGC TCAGCCAGTC GCAGCCGCAC ATTGTGCCGC CCAGCATACC GCCGCACCAC 
CACCACCATC GCGAGAACAA TGTGCACCCC ATCCAGGCGG GCGCGGCAGC CGCATTGCAT 
TTGTCATCGC ATGCGCTTAA TAGTCATAAT TTAATCAGCA GCGCCAGCAA TCATTCGCCC 
AAGTCCCCCA GCCAGACGCA GCAGCAGCAA CAGACGACGA ACCAGCAGCA ACAACAGCAG 
CAGCAGCAGG CGTCCCACAG CATACTGTCC ACGTTTGCGC CCGCCCCCAC TGTCGCCGCC 
GTTCAGCCGG CGGGATCGCA GTCCGTTGCC GGTACCGCTG CCGCAGTGGC CGCCGTTGCC 
CAGCAGCAGC AACAGCAGCA TCTTCAGCTG CTGGCCAACT GTCCGCCCGG TGGAGCGGGT 
CTAATGTCCT CCTCCCTGAA TGCAGCCCAA ATGGGCATGC ATGGCGCCGA GCGAGCGCTG 
CCGCCAAAGA GTCTGGCGCT GAGTGGCAGC CAGCACGGCA GCAATATGCT ACTGCACACA 
GCACCGCAAC CGCCACAGGC ATCCCAGCCA GCGGCAGTCG TCGGCGTCAG TGGTAGCGGA 
TCGGCGGGTA TGTCCACCTC GCTGCACAGC TTCGATATTA ATGGAGTCAC GGCAGGCAGC 
GGGGTGAATG CGAGCAGCGC GTGGTCAAGC GGATCCACCA CTGCCATGAA CCATGCTTCC 
CTCTCGCCCA CAGCGTTGGC ACCGCAACAA GGTCGGTCCA GGTGCGAGGT CAAACTGAAC 
GCCATGCCAT GGTTCCATGG CAGCATAACG CGGGATGAGG CGGAGCATCT GCTGCAGCCG 
CGTGAGGATG GTTTGTTCCT GGTTCGCGAG TCCACAAACT TCCCCGGCGA CTACACGCTC 
TGCGTTTGTT TCCAGTCCAA GGTGGAGCAC TACCGGGTCA AGTACCTAGA GAACAAGCTG 
ACCATCGACG ACGAGGAATA CTTTGAGAAT TTGGGCCAGC TAGTGGCGCA CTACGAGGCG 
GATGCGGATG GACTGTGCAC CCAGCTGATT AAATGCTTGC CAAAGCTGGG CAAACAAGAG 
TTTTGCATTA ACTCCAAAGA TTTCGTGGAC AAGGGCTGGG TGATCCCTGA AGCGGAGCTG 
CAGCTGCGCG AGAGCATCGG CAAGGGCGAG TTCGGTGACG TGATGCTGGG CATATTACGC 
AACGAGAAGG TGGCCGTCAA GATGCTGAAG GACGAGGGCG CCGTGCAGAA GTTTCTGGCT 
GAGGCCTCGG TCATGACCAC GCTGGAGCAC GACAATTTGG TAAAGTTTAT CGGCCTTGTC 
TTCACCAGCA AGCATCTGTA TCTGGTTACG GAATATATGA GTAAGGGATC ACTGGTTGAT 
TACCTGCGAT CGCGAGGACG ACAGCACATA ACCAAAAAGG ATCAAATCAT TTTTGCCTAC 
GACACTGCCT CTGGTATGGA GTATCTGGAA GCCAAAAAGG TTGTCCATCG CGATCTGGCA 
GCTCGCAATG TCCTTATATC AGAGGATTGC GTGGCTAAAG TATCGGATTT CGGTTTGGCA 
CGCGAGGAAT GCTACAATCT TGACGTTGGC AAGCTGCCCA TAAAATGGAC GGCTCCCGAG 
GCACTTAAAA ATGGGCGTTT TTCCAACAAA TCAGACATGT GGAGCTTTGG CATACTGCTA 
TGGGAAATCT ACTCCTTTGG ACGCGTGCCC TATCCCAGAA TTCCATTAGC CGATGTGGTA 
AAGCACGTTG AGGTGGGCTA CAAAATGGAA GCGCCCGAGG GCTGTCCACC GGAGATTTAC 
GAAATGATGC GGCAGGCCTG GGACCTAAAT CCCGCCAAGC GACCCACATT CGCGGAGCTC 
AAGGTCAAAC TGCAGCTACT GAACAATGCC ACGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262476.1
CDS	132..3290
Translation	MVTTIKITCDGVFIQSNSYLMPTPSPSGADATVSSSLERVLKPASASASPSNSNNESDNSQTSSGSQTASSASKRSNRSVGRSHRGSIAQDTRRRYYFGSKDIYWCKQSREAAAAATLSGSGLTRSASSVTHKEASTHATHTGGQVISRARRKPILLCSSLSAAYAPPRRREFYFCDKYAPGPARSTPTTTTTSATSAKHESSPPIDARLLNFLRCAHRKFQASKAKTELQQTKDRFLGNSRERTSKNGQQTSRSLPPIASSEEWFDEEAQARKTSHEKSQKPKPKPEENHKEKEAISRKASGEGDLITVEIKQGLLRKQTATTTTGQAMNSHATATQNGQTTVVGHHRGFSQPSVTVAAAAATGSTAGDPWFLQTTGHQMPPTAPLRHNKRPAPQPNAVLGAGSSGTAPGSAVGAVLLHQQQLSQSQPHIVPPSIPPHHHHHRENNVHPIQAGAAAALHLSSHALNSHNLISSASNHSPKSPSQTQQQQQTTNQQQQQQQQQASHSILSTFAPAPTVAAVQPAGSQSVAGTAAAVAAVAQQQQQQHLQLLANCPPGGAGLMSSSLNAAQMGMHGAERALPPKSLALSGSQHGSNMLLHTAPQPPQASQPAAVVGVSGSGSAGMSTSLHSFDINGVTAGSGVNASSAWSSGSTTAMNHASLSPTALAPQQGRSRCEVKLNAMPWFHGSITRDEAEHLLQPREDGLFLVRESTNFPGDYTLCVCFQSKVEHYRVKYLENKLTIDDEEYFENLGQLVAHYEADADGLCTQLIKCLPKLGKQEFCINSKDFVDKGWVIPEAELQLRESIGKGEFGDVMLGILRNEKVAVKMLKDEGAVQKFLAEASVMTTLEHDNLVKFIGLVFTSKHLYLVTEYMSKGSLVDYLRSRGRQHITKKDQIIFAYDTASGMEYLEAKKVVHRDLAARNVLISEDCVAKVSDFGLAREECYNLDVGKLPIKWTAPEALKNGRFSNKSDMWSFGILLWEIYSFGRVPYPRIPLADVVKHVEVGYKMEAPEGCPPEIYEMMRQAWDLNPAKRPTFAELKVKLQLLNNATA

Target ORF information:

RefSeq Version	NM_001275547.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster C-terminal Src kinase (Csk), transcript variant L, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275547.1

ORF Insert Sequence:

ATGGTGACAA CAATTAAGAT AACCTGCGAT GGAGTGTTCA TCCAGTCCAA CTCGTACCTC 
ATGCCCACGC CTTCTCCCAG CGGAGCAGAC GCCACGGTAT CGTCATCCTT GGAACGGGTG 
CTGAAACCCG CGTCCGCATC CGCCTCTCCA TCCAATTCGA ACAACGAAAG TGATAACTCG 
CAGACGAGCA GTGGCAGCCA GACGGCGTCC AGTGCCAGCA AGCGATCCAA TCGATCCGTG 
GGCCGGAGTC ATCGAGGTTC GATTGCGCAG GACACAAGGC GACGCTACTA CTTTGGCAGC 
AAGGACATCT ACTGGTGCAA GCAGAGCAGG GAGGCAGCTG CGGCGGCCAC GCTCTCGGGC 
TCGGGACTAA CGCGTAGTGC CAGCAGTGTC ACCCACAAGG AAGCGTCCAC CCACGCCACC 
CACACTGGTG GTCAGGTAAT CTCCCGTGCG CGTCGCAAGC CCATCCTGCT TTGCAGCAGC 
CTGAGCGCTG CCTATGCTCC TCCTCGTCGG CGTGAGTTTT ACTTCTGCGA CAAGTATGCA 
CCTGGTCCAG CAAGAAGTAC TCCTACTACA ACCACAACAT CCGCAACATC GGCCAAACAC 
GAATCCTCCC CGCCCATCGA CGCACGTCTG CTGAACTTCC TGCGGTGCGC ACATCGAAAG 
TTCCAGGCAT CCAAAGCCAA AACTGAGCTA CAGCAAACCA AAGACAGATT CCTGGGAAAT 
TCCAGAGAGC GGACTAGTAA GAACGGGCAG CAGACGTCCC GCTCGCTGCC ACCCATTGCC 
AGTTCGGAGG AGTGGTTCGA CGAGGAGGCA CAAGCCAGGA AGACTAGCCA CGAGAAGAGC 
CAGAAGCCCA AGCCGAAGCC GGAGGAAAAC CATAAGGAGA AGGAAGCGAT CTCGAGAAAG 
GCGTCCGGCG AGGGTGATCT GATCACCGTT GAGATCAAGC AGGGATTGCT GAGGAAGCAG 
ACGGCAACCA CGACGACCGG CCAGGCTATG AACAGCCACG CGACTGCCAC GCAGAACGGA 
CAAACCACGG TCGTCGGACA CCATCGCGGA TTCTCGCAGC CCTCGGTGAC GGTGGCCGCT 
GCAGCGGCAA CGGGCAGCAC GGCTGGTGAT CCATGGTTCC TGCAGACCAC CGGTCACCAG 
ATGCCACCCA CTGCACCGTT GCGGCACAAC AAGCGTCCTG CCCCCCAGCC GAATGCGGTT 
TTGGGAGCGG GTTCCTCGGG AACAGCGCCT GGATCGGCGG TTGGCGCCGT ACTGCTCCAC 
CAACAGCAGC TCAGCCAGTC GCAGCCGCAC ATTGTGCCGC CCAGCATACC GCCGCACCAC 
CACCACCATC GCGAGAACAA TGTGCACCCC ATCCAGGCGG GCGCGGCAGC CGCATTGCAT 
TTGTCATCGC ATGCGCTTAA TAGTCATAAT TTAATCAGCA GCGCCAGCAA TCATTCGCCC 
AAGTCCCCCA GCCAGACGCA GCAGCAGCAA CAGACGACGA ACCAGCAGCA ACAACAGCAG 
CAGCAGCAGG CGTCCCACAG CATACTGTCC ACGTTTGCGC CCGCCCCCAC TGTCGCCGCC 
GTTCAGCCGG CGGGATCGCA GTCCGTTGCC GGTACCGCTG CCGCAGTGGC CGCCGTTGCC 
CAGCAGCAGC AACAGCAGCA TCTTCAGCTG CTGGCCAACT GTCCGCCCGG TGGAGCGGGT 
CTAATGTCCT CCTCCCTGAA TGCAGCCCAA ATGGGCATGC ATGGCGCCGA GCGAGCGCTG 
CCGCCAAAGA GTCTGGCGCT GAGTGGCAGC CAGCACGGCA GCAATATGCT ACTGCACACA 
GCACCGCAAC CGCCACAGGC ATCCCAGCCA GCGGCAGTCG TCGGCGTCAG TGGTAGCGGA 
TCGGCGGGTA TGTCCACCTC GCTGCACAGC TTCGATATTA ATGGAGTCAC GGCAGGCAGC 
GGGGTGAATG CGAGCAGCGC GTGGTCAAGC GGATCCACCA CTGCCATGAA CCATGCTTCC 
CTCTCGCCCA CAGCGTTGGC ACCGCAACAA GGTCGGTCCA GGTGCGAGGT CAAACTGAAC 
GCCATGCCAT GGTTCCATGG CAGCATAACG CGGGATGAGG CGGAGCATCT GCTGCAGCCG 
CGTGAGGATG GTTTGTTCCT GGTTCGCGAG TCCACAAACT TCCCCGGCGA CTACACGCTC 
TGCGTTTGTT TCCAGTCCAA GGTGGAGCAC TACCGGGTCA AGTACCTAGA GAACAAGCTG 
ACCATCGACG ACGAGGAATA CTTTGAGAAT TTGGGCCAGC TAGTGGCGCA CTACGAGGCG 
GATGCGGATG GACTGTGCAC CCAGCTGATT AAATGCTTGC CAAAGCTGGG CAAACAAGAG 
TTTTGCATTA ACTCCAAAGA TTTCGTGGAC AAGGGCTGGG TGATCCCTGA AGCGGAGCTG 
CAGCTGCGCG AGAGCATCGG CAAGGGCGAG TTCGGTGACG TGATGCTGGG CATATTACGC 
AACGAGAAGG TGGCCGTCAA GATGCTGAAG GACGAGGGCG CCGTGCAGAA GTTTCTGGCT 
GAGGCCTCGG TCATGACCAC GCTGGAGCAC GACAATTTGG TAAAGTTTAT CGGCCTTGTC 
TTCACCAGCA AGCATCTGTA TCTGGTTACG GAATATATGA GTAAGGGATC ACTGGTTGAT 
TACCTGCGAT CGCGAGGACG ACAGCACATA ACCAAAAAGG ATCAAATCAT TTTTGCCTAC 
GACACTGCCT CTGGTATGGA GTATCTGGAA GCCAAAAAGG TTGTCCATCG CGATCTGGCA 
GCTCGCAATG TCCTTATATC AGAGGATTGC GTGGCTAAAG TATCGGATTT CGGTTTGGCA 
CGCGAGGAAT GCTACAATCT TGACGTTGGC AAGCTGCCCA TAAAATGGAC GGCTCCCGAG 
GCACTTAAAA ATGGGCGTTT TTCCAACAAA TCAGACATGT GGAGCTTTGG CATACTGCTA 
TGGGAAATCT ACTCCTTTGG ACGCGTGCCC TATCCCAGAA TTCCATTAGC CGATGTGGTA 
AAGCACGTTG AGGTGGGCTA CAAAATGGAA GCGCCCGAGG GCTGTCCACC GGAGATTTAC 
GAAATGATGC GGCAGGCCTG GGACCTAAAT CCCGCCAAGC GACCCACATT CGCGGAGCTC 
AAGGTCAAAC TGCAGCTACT GAACAATGCC ACGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Csk ( NM_141840.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650097.2 Drosophila melanogaster C-terminal Src kinase (Csk), transcript variant H, mRNA.

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CloneID	OFa04556
Clone ID Related Accession (Same CDS sequence)	NM_141840.1
Accession Version	NM_141840.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	C-terminal Src kinase, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGAAT CGATTGCAAT TGGCTTTTTG CAATACATAA TAACAATAAA TTATGACGAC 
GAGGAGGCAC AAGCCAGGAA GACTAGCCAC GAGAAGAGCC AGAAGCCCAA GCCGAAGCCG 
GAGGAAAACC ATAAGGAGAA GGAAGCGATC TCGAGAAAGG CGTCCGGCGA GGGTGATCTG 
ATCACCGTTG AGATCAAGCA GGGATTGCTG AGGAAGCAGA CGGCAACCAC GACGACCGGC 
CAGGCTATGA ACAGCCACGC GACTGCCACG CAGAACGGAC AAACCACGGT CGTCGGACAC 
CATCGCGGAT TCTCGCAGCC CTCGGTGACG GTGGCCGCTG CAGCGGCAAC GGGCAGCACG 
GCTGGTGATC CATGGTTCCT GCAGACCACC GGTCACCAGA TGCCACCCAC TGCACCGTTG 
CGGCACAACA AGCGTCCTGC CCCCCAGCCG AATGCGGTTT TGGGAGCGGG TTCCTCGGGA 
ACAGCGCCTG GATCGGCGGT TGGCGCCGTA CTGCTCCACC AACAGCAGCT CAGCCAGTCG 
CAGCCGCACA TTGTGCCGCC CAGCATACCG CCGCACCACC ACCACCATCG CGAGAACAAT 
GTGCACCCCA TCCAGGCGGG CGCGGCAGCC GCATTGCATT TGTCATCGCA TGCGCTTAAT 
AGTCATAATT TAATCAGCAG CGCCAGCAAT CATTCGCCCA AGTCCCCCAG CCAGACGCAG 
CAGCAGCAAC AGACGACGAA CCAGCAGCAA CAACAGCAGC AGCAGCAGGC GTCCCACAGC 
ATACTGTCCA CGTTTGCGCC CGCCCCCACT GTCGCCGCCG TTCAGCCGGC GGGATCGCAG 
TCCGTTGCCG GTACCGCTGC CGCAGTGGCC GCCGTTGCCC AGCAGCAGCA ACAGCAGCAT 
CTTCAGCTGC TGGCCAACTG TCCGCCCGGT GGAGCGGGTC TAATGTCCTC CTCCCTGAAT 
GCAGCCCAAA TGGGCATGCA TGGCGCCGAG CGAGCGCTGC CGCCAAAGAG TCTGGCGCTG 
AGTGGCAGCC AGCACGGCAG CAATATGCTA CTGCACACAG CACCGCAACC GCCACAGGCA 
TCCCAGCCAG CGGCAGTCGT CGGCGTCAGT GGTAGCGGAT CGGCGGGTAT GTCCACCTCG 
CTGCACAGCT TCGATATTAA TGGAGTCACG GCAGGCAGCG GGGTGAATGC GAGCAGCGCG 
TGGTCAAGCG GATCCACCAC TGCCATGAAC CATGCTTCCC TCTCGCCCAC AGCGTTGGCA 
CCGCAACAAG GTCGGTCCAG GTGCGAGGTC AAACTGAACG CCATGCCATG GTTCCATGGC 
AGCATAACGC GGGATGAGGC GGAGCATCTG CTGCAGCCGC GTGAGGATGG TTTGTTCCTG 
GTTCGCGAGT CCACAAACTT CCCCGGCGAC TACACGCTCT GCGTTTGTTT CCAGTCCAAG 
GTGGAGCACT ACCGGGTCAA GTACCTAGAG AACAAGCTGA CCATCGACGA CGAGGAATAC 
TTTGAGAATT TGGGCCAGCT AGTGGCGCAC TACGAGGCGG ATGCGGATGG ACTGTGCACC 
CAGCTGATTA AATGCTTGCC AAAGCTGGGC AAACAAGAGT TTTGCATTAA CTCCAAAGAT 
TTCGTGGACA AGGGCTGGGT GATCCCTGAA GCGGAGCTGC AGCTGCGCGA GAGCATCGGC 
AAGGGCGAGT TCGGTGACGT GATGCTGGGC ATATTACGCA ACGAGAAGGT GGCCGTCAAG 
ATGCTGAAGG ACGAGGGCGC CGTGCAGAAG TTTCTGGCTG AGGCCTCGGT CATGACCACG 
CTGGAGCACG ACAATTTGGT AAAGTTTATC GGCCTTGTCT TCACCAGCAA GCATCTGTAT 
CTGGTTACGG AATATATGAG TAAGGGATCA CTGGTTGATT ACCTGCGATC GCGAGGACGA 
CAGCACATAA CCAAAAAGGA TCAAATCATT TTTGCCTACG ACACTGCCTC TGGTATGGAG 
TATCTGGAAG CCAAAAAGGT TGTCCATCGC GATCTGGCAG CTCGCAATGT CCTTATATCA 
GAGGATTGCG TGGCTAAAGT ATCGGATTTC GGTTTGGCAC GCGAGGAATG CTACAATCTT 
GACGTTGGCA AGCTGCCCAT AAAATGGACG GCTCCCGAGG CACTTAAAAA TGGGCGTTTT 
TCCAACAAAT CAGACATGTG GAGCTTTGGC ATACTGCTAT GGGAAATCTA CTCCTTTGGA 
CGCGTGCCCT ATCCCAGAAT TCCATTAGCC GATGTGGTAA AGCACGTTGA GGTGGGCTAC 
AAAATGGAAG CGCCCGAGGG CTGTCCACCG GAGATTTACG AAATGATGCG GCAGGCCTGG 
GACCTAAATC CCGCCAAGCG ACCCACATTC GCGGAGCTCA AGGTCAAACT GCAGCTACTG 
AACAATGCCA CGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650097.2
CDS	129..2546
Translation	MSESIAIGFLQYIITINYDDEEAQARKTSHEKSQKPKPKPEENHKEKEAISRKASGEGDLITVEIKQGLLRKQTATTTTGQAMNSHATATQNGQTTVVGHHRGFSQPSVTVAAAAATGSTAGDPWFLQTTGHQMPPTAPLRHNKRPAPQPNAVLGAGSSGTAPGSAVGAVLLHQQQLSQSQPHIVPPSIPPHHHHHRENNVHPIQAGAAAALHLSSHALNSHNLISSASNHSPKSPSQTQQQQQTTNQQQQQQQQQASHSILSTFAPAPTVAAVQPAGSQSVAGTAAAVAAVAQQQQQQHLQLLANCPPGGAGLMSSSLNAAQMGMHGAERALPPKSLALSGSQHGSNMLLHTAPQPPQASQPAAVVGVSGSGSAGMSTSLHSFDINGVTAGSGVNASSAWSSGSTTAMNHASLSPTALAPQQGRSRCEVKLNAMPWFHGSITRDEAEHLLQPREDGLFLVRESTNFPGDYTLCVCFQSKVEHYRVKYLENKLTIDDEEYFENLGQLVAHYEADADGLCTQLIKCLPKLGKQEFCINSKDFVDKGWVIPEAELQLRESIGKGEFGDVMLGILRNEKVAVKMLKDEGAVQKFLAEASVMTTLEHDNLVKFIGLVFTSKHLYLVTEYMSKGSLVDYLRSRGRQHITKKDQIIFAYDTASGMEYLEAKKVVHRDLAARNVLISEDCVAKVSDFGLAREECYNLDVGKLPIKWTAPEALKNGRFSNKSDMWSFGILLWEIYSFGRVPYPRIPLADVVKHVEVGYKMEAPEGCPPEIYEMMRQAWDLNPAKRPTFAELKVKLQLLNNATA

Target ORF information:

RefSeq Version	NM_141840.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster C-terminal Src kinase (Csk), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141840.1

ORF Insert Sequence:

ATGAGTGAAT CGATTGCAAT TGGCTTTTTG CAATACATAA TAACAATAAA TTATGACGAC 
GAGGAGGCAC AAGCCAGGAA GACTAGCCAC GAGAAGAGCC AGAAGCCCAA GCCGAAGCCG 
GAGGAAAACC ATAAGGAGAA GGAAGCGATC TCGAGAAAGG CGTCCGGCGA GGGTGATCTG 
ATCACCGTTG AGATCAAGCA GGGATTGCTG AGGAAGCAGA CGGCAACCAC GACGACCGGC 
CAGGCTATGA ACAGCCACGC GACTGCCACG CAGAACGGAC AAACCACGGT CGTCGGACAC 
CATCGCGGAT TCTCGCAGCC CTCGGTGACG GTGGCCGCTG CAGCGGCAAC GGGCAGCACG 
GCTGGTGATC CATGGTTCCT GCAGACCACC GGTCACCAGA TGCCACCCAC TGCACCGTTG 
CGGCACAACA AGCGTCCTGC CCCCCAGCCG AATGCGGTTT TGGGAGCGGG TTCCTCGGGA 
ACAGCGCCTG GATCGGCGGT TGGCGCCGTA CTGCTCCACC AACAGCAGCT CAGCCAGTCG 
CAGCCGCACA TTGTGCCGCC CAGCATACCG CCGCACCACC ACCACCATCG CGAGAACAAT 
GTGCACCCCA TCCAGGCGGG CGCGGCAGCC GCATTGCATT TGTCATCGCA TGCGCTTAAT 
AGTCATAATT TAATCAGCAG CGCCAGCAAT CATTCGCCCA AGTCCCCCAG CCAGACGCAG 
CAGCAGCAAC AGACGACGAA CCAGCAGCAA CAACAGCAGC AGCAGCAGGC GTCCCACAGC 
ATACTGTCCA CGTTTGCGCC CGCCCCCACT GTCGCCGCCG TTCAGCCGGC GGGATCGCAG 
TCCGTTGCCG GTACCGCTGC CGCAGTGGCC GCCGTTGCCC AGCAGCAGCA ACAGCAGCAT 
CTTCAGCTGC TGGCCAACTG TCCGCCCGGT GGAGCGGGTC TAATGTCCTC CTCCCTGAAT 
GCAGCCCAAA TGGGCATGCA TGGCGCCGAG CGAGCGCTGC CGCCAAAGAG TCTGGCGCTG 
AGTGGCAGCC AGCACGGCAG CAATATGCTA CTGCACACAG CACCGCAACC GCCACAGGCA 
TCCCAGCCAG CGGCAGTCGT CGGCGTCAGT GGTAGCGGAT CGGCGGGTAT GTCCACCTCG 
CTGCACAGCT TCGATATTAA TGGAGTCACG GCAGGCAGCG GGGTGAATGC GAGCAGCGCG 
TGGTCAAGCG GATCCACCAC TGCCATGAAC CATGCTTCCC TCTCGCCCAC AGCGTTGGCA 
CCGCAACAAG GTCGGTCCAG GTGCGAGGTC AAACTGAACG CCATGCCATG GTTCCATGGC 
AGCATAACGC GGGATGAGGC GGAGCATCTG CTGCAGCCGC GTGAGGATGG TTTGTTCCTG 
GTTCGCGAGT CCACAAACTT CCCCGGCGAC TACACGCTCT GCGTTTGTTT CCAGTCCAAG 
GTGGAGCACT ACCGGGTCAA GTACCTAGAG AACAAGCTGA CCATCGACGA CGAGGAATAC 
TTTGAGAATT TGGGCCAGCT AGTGGCGCAC TACGAGGCGG ATGCGGATGG ACTGTGCACC 
CAGCTGATTA AATGCTTGCC AAAGCTGGGC AAACAAGAGT TTTGCATTAA CTCCAAAGAT 
TTCGTGGACA AGGGCTGGGT GATCCCTGAA GCGGAGCTGC AGCTGCGCGA GAGCATCGGC 
AAGGGCGAGT TCGGTGACGT GATGCTGGGC ATATTACGCA ACGAGAAGGT GGCCGTCAAG 
ATGCTGAAGG ACGAGGGCGC CGTGCAGAAG TTTCTGGCTG AGGCCTCGGT CATGACCACG 
CTGGAGCACG ACAATTTGGT AAAGTTTATC GGCCTTGTCT TCACCAGCAA GCATCTGTAT 
CTGGTTACGG AATATATGAG TAAGGGATCA CTGGTTGATT ACCTGCGATC GCGAGGACGA 
CAGCACATAA CCAAAAAGGA TCAAATCATT TTTGCCTACG ACACTGCCTC TGGTATGGAG 
TATCTGGAAG CCAAAAAGGT TGTCCATCGC GATCTGGCAG CTCGCAATGT CCTTATATCA 
GAGGATTGCG TGGCTAAAGT ATCGGATTTC GGTTTGGCAC GCGAGGAATG CTACAATCTT 
GACGTTGGCA AGCTGCCCAT AAAATGGACG GCTCCCGAGG CACTTAAAAA TGGGCGTTTT 
TCCAACAAAT CAGACATGTG GAGCTTTGGC ATACTGCTAT GGGAAATCTA CTCCTTTGGA 
CGCGTGCCCT ATCCCAGAAT TCCATTAGCC GATGTGGTAA AGCACGTTGA GGTGGGCTAC 
AAAATGGAAG CGCCCGAGGG CTGTCCACCG GAGATTTACG AAATGATGCG GCAGGCCTGG 
GACCTAAATC CCGCCAAGCG ACCCACATTC GCGGAGCTCA AGGTCAAACT GCAGCTACTG 
AACAATGCCA CGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Csk cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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