Pi3K68D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pi3K68D
Entrez Gene ID	39329
Full Name	Phosphotidylinositol 3 kinase 68D
Synonyms	11621,BcDNA:LD15217,CG11621,Cpk,Dmel\CG11621,PI(3)K,PI3K,PI3K 68D,PI3K 68_D,PI3K-68D,PI3K-68D/E,PI3K68D,PI3K_68D,PI[[3]]K,Pi3K,cpk,dPI3K,dPI3k,dPIK,pi3k68d
General protein information	Preferred Names Phosphotidylinositol 3 kinase 68D Names CG11621 gene product from transcript CG11621-RD CG11621-PA CG11621-PB CG11621-PC CG11621-PD CG11621-PE PI 3-kinase PI3-kinase Pi3K68D-PA Pi3K68D-PB Pi3K68D-PC Pi3K68D-PD Pi3K68D-PE dPI 3-kinase phosphotidylinositol 3 kinase 68D
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 68D4-68D6

mRNA and Protein(s)

mRNA	Protein	Name
NM_168475.3	NP_729743.1	phosphotidylinositol 3 kinase 68D, isoform C
NM_168476.3	NP_729745.1	phosphotidylinositol 3 kinase 68D, isoform B
NM_001169949.2	NP_001163420.1	phosphotidylinositol 3 kinase 68D, isoform D
NM_001274795.1	NP_001261724.1	phosphotidylinositol 3 kinase 68D, isoform E
NM_079304.4	NP_524028.2	phosphotidylinositol 3 kinase 68D, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	PIK3C2A	NP_002636.2
Canis lupus familiaris (dog)	PIK3C2A	XP_005633773.1
Bos taurus (cattle)	PIK3C2A	XP_002693140.2
Mus musculus (house mouse)	Pik3c2a	NP_035213.2
Danio rerio (zebrafish)	pik3c2a	XP_005159363.1
Caenorhabditis elegans (roundworm)	piki-1	NP_510529.1
	LOC697202	XP_002808122.1
Rattus norvegicus (Norway rat)	Pik3c2a	NP_001101970.1
Gallus gallus (chicken)	PIK3C2A	XP_421003.2
Xenopus tropicalis (tropical clawed frog)	pik3c2a	NP_001128289.1
Drosophila melanogaster (fruit fly)	Pi3K68D	NP_524028.2
Pan troglodytes (chimpanzee)	PIK3C2A	XP_001172532.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011036	XP_001237042.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results highlight an unexpected crosstalk whereby the normally growth-promoting, nutrient-sensing PI3K/TOR pathway suppresses tumour formation in neural stem cells with compromised cell polarity.
Title: Synergism between altered cortical polarity and the PI3K/TOR pathway in the suppression of tumour growth.

study concludes that tumor cells become dependent on PI3K function and that reduction of PI3K activity synthetically interferes with tumor growth.
Title: Loss of PI3K blocks cell-cycle progression in a Drosophila tumor model.

evidence for a biological function for a class II PI3K by expressing this enzyme during development and by using deficiencies that remove the endogenous gene
Title: Targeted expression of the class II phosphoinositide 3-kinase in Drosophila melanogaster reveals lipid kinase-dependent effects on patterning and interactions with receptor signaling pathways.

inhibiting Inr/PI3K signaling phenocopies the cellular and organismal effects of starvation, whereas activating this pathway bypasses the nutritional requirement for cell growth, causing starvation sensitivity at the organismal level
Title: Drosophila's insulin/PI3-kinase pathway coordinates cellular metabolism with nutritional conditions.

The following Pi3K68D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pi3K68D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18285	NM_168476.3 Latest version!	Drosophila melanogaster phosphotidylinositol 3 kinase 68D, transcript variant B (Pi3K68D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa18285	NM_001274795.1 Latest version!	Drosophila melanogaster phosphotidylinositol 3 kinase 68D, transcript variant E (Pi3K68D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa18284	NM_079304.4 Latest version!	Drosophila melanogaster phosphotidylinositol 3 kinase 68D, transcript variant A (Pi3K68D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa18284	NM_168475.3 Latest version!	Drosophila melanogaster phosphotidylinositol 3 kinase 68D, transcript variant C (Pi3K68D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa18284	NM_001169949.2 Latest version!	Drosophila melanogaster phosphotidylinositol 3 kinase 68D, transcript variant D (Pi3K68D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pi3K68D ( NM_168476.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729745.1 Drosophila melanogaster phosphotidylinositol 3 kinase 68D, transcript variant B (Pi3K68D), mRNA.

Price & Availability↑

CloneID	OFa18285
Clone ID Related Accession (Same CDS sequence)	NM_168476.3 , NM_001274795.1
Accession Version	NM_168476.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphotidylinositol 3 kinase 68D, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168476.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAAATC AAGCGCATAT CGACTACGAC AAACAATTCC AGGATGACCT GGCCAAGGCG 
ACCGCCCTGA GTCTAGAGCA GCATGCCCTC GATGACTACA GGCGAAACAA GAAGTACGGC 
TCCGGGTATC AGCAAAGCTC CACCGTTGCT GGCCGAGATT ACCAGGCGGC GCAACGTAGT 
CAGAGCCTAC ATCAACCACG ACGGCACTCG GAGGTGCATC AGGTGGCCAT CAGTCCGGAG 
AATGCGGAAC GATCGCGCAC ACCGCCGGCC CAGGGAACGG ATAACGATCT GATCTGCTTC 
GCAAGTCCCA CCAGCAAGCA GCCAGAGAGT AGCAGTCCCT TTGGCAAACT TATAGAGGAT 
CTGCAGCGGA TGCAGCCGAC CAATCCGCAG TCGGCCCTGG TGCCAATGGG TCCAGTTGCG 
TCGGCTTCGA TTCCTCCTCA ATACGGCTTT CCACCTCATC AGCAACGTCC AACGGCTGCT 
CAGCCCACAC CGTACGGCAT GGTTGCAGGT GGAGTTGTTG GTGGACCGGC TTACGGTGAC 
CTGCAGTTGG TGCCTTACCA ACCAGCTGCT CAGCAACAGA GGCCACTAAA CAGCGAGGAG 
CTGCAGCGGC TGTACAGCAT GCCCGCTCAA ATGGCCGTGG TTCCAGTGCC GCAGCCAAAC 
GCCTATATGT ACTATCCCGG AGCTGTGGTT ACTCCATACA CGGCTCCCAT TGTTCCCGGA 
TCGGCTGCTT TTATGCCGCC GCAGTATCCC GCACAGGGAT ATGGCTTTGG AGGTGCTTAC 
ACGCACATGG ATTTGCGTCG ACCCCAATCG CAACCAGCTC CCCAACAAAC AGCACCGACA 
ACAAGTCATC ATCACAGCCA ACCGTCCAAC CATTCCACTT CCTCCCCCGC AGAGGCCAAT 
GGAGTAGCCT TCCCAGCGCG TCGCCAAGTG CCCTCGACTG TCGGGGTTAG CTCTAGTAGC 
CACACTGGAA ACAATGGTCA TTCCTCGGTC CCACGCAGGG GCAACGATTT GATCGACCTC 
AACCACGAGG ACTACTCCCG TGTGAGTGTG CTGGAGGCAT TCGATCCCCT GCTAAACGAC 
AATACTGTGT CGAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729745.1
CDS	206..1306
Translation	MSNQAHIDYDKQFQDDLAKATALSLEQHALDDYRRNKKYGSGYQQSSTVAGRDYQAAQRSQSLHQPRRHSEVHQVAISPENAERSRTPPAQGTDNDLICFASPTSKQPESSSPFGKLIEDLQRMQPTNPQSALVPMGPVASASIPPQYGFPPHQQRPTAAQPTPYGMVAGGVVGGPAYGDLQLVPYQPAAQQQRPLNSEELQRLYSMPAQMAVVPVPQPNAYMYYPGAVVTPYTAPIVPGSAAFMPPQYPAQGYGFGGAYTHMDLRRPQSQPAPQQTAPTTSHHHSQPSNHSTSSPAEANGVAFPARRQVPSTVGVSSSSHTGNNGHSSVPRRGNDLIDLNHEDYSRVSVLEAFDPLLNDNTVSKK

Target ORF information:

RefSeq Version	NM_168476.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphotidylinositol 3 kinase 68D, transcript variant B (Pi3K68D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168476.3

ORF Insert Sequence:

ATGTCAAATC AAGCGCATAT CGACTACGAC AAACAATTCC AGGATGACCT GGCCAAGGCG 
ACCGCCCTGA GTCTAGAGCA GCATGCCCTC GATGACTACA GGCGAAACAA GAAGTACGGC 
TCCGGGTATC AGCAAAGCTC CACCGTTGCT GGCCGAGATT ACCAGGCGGC GCAACGTAGT 
CAGAGCCTAC ATCAACCACG ACGGCACTCG GAGGTGCATC AGGTGGCCAT CAGTCCGGAG 
AATGCGGAAC GATCGCGCAC ACCGCCGGCC CAGGGAACGG ATAACGATCT GATCTGCTTC 
GCAAGTCCCA CCAGCAAGCA GCCAGAGAGT AGCAGTCCCT TTGGCAAACT TATAGAGGAT 
CTGCAGCGGA TGCAGCCGAC CAATCCGCAG TCGGCCCTGG TGCCAATGGG TCCAGTTGCG 
TCGGCTTCGA TTCCTCCTCA ATACGGCTTT CCACCTCATC AGCAACGTCC AACGGCTGCT 
CAGCCCACAC CGTACGGCAT GGTTGCAGGT GGAGTTGTTG GTGGACCGGC TTACGGTGAC 
CTGCAGTTGG TGCCTTACCA ACCAGCTGCT CAGCAACAGA GGCCACTAAA CAGCGAGGAG 
CTGCAGCGGC TGTACAGCAT GCCCGCTCAA ATGGCCGTGG TTCCAGTGCC GCAGCCAAAC 
GCCTATATGT ACTATCCCGG AGCTGTGGTT ACTCCATACA CGGCTCCCAT TGTTCCCGGA 
TCGGCTGCTT TTATGCCGCC GCAGTATCCC GCACAGGGAT ATGGCTTTGG AGGTGCTTAC 
ACGCACATGG ATTTGCGTCG ACCCCAATCG CAACCAGCTC CCCAACAAAC AGCACCGACA 
ACAAGTCATC ATCACAGCCA ACCGTCCAAC CATTCCACTT CCTCCCCCGC AGAGGCCAAT 
GGAGTAGCCT TCCCAGCGCG TCGCCAAGTG CCCTCGACTG TCGGGGTTAG CTCTAGTAGC 
CACACTGGAA ACAATGGTCA TTCCTCGGTC CCACGCAGGG GCAACGATTT GATCGACCTC 
AACCACGAGG ACTACTCCCG TGTGAGTGTG CTGGAGGCAT TCGATCCCCT GCTAAACGAC 
AATACTGTGT CGAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pi3K68D ( NM_001274795.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261724.1 Drosophila melanogaster phosphotidylinositol 3 kinase 68D, transcript variant E (Pi3K68D), mRNA.

Price & Availability↑

CloneID	OFa18285
Clone ID Related Accession (Same CDS sequence)	NM_168476.3 , NM_001274795.1
Accession Version	NM_001274795.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphotidylinositol 3 kinase 68D, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAAATC AAGCGCATAT CGACTACGAC AAACAATTCC AGGATGACCT GGCCAAGGCG 
ACCGCCCTGA GTCTAGAGCA GCATGCCCTC GATGACTACA GGCGAAACAA GAAGTACGGC 
TCCGGGTATC AGCAAAGCTC CACCGTTGCT GGCCGAGATT ACCAGGCGGC GCAACGTAGT 
CAGAGCCTAC ATCAACCACG ACGGCACTCG GAGGTGCATC AGGTGGCCAT CAGTCCGGAG 
AATGCGGAAC GATCGCGCAC ACCGCCGGCC CAGGGAACGG ATAACGATCT GATCTGCTTC 
GCAAGTCCCA CCAGCAAGCA GCCAGAGAGT AGCAGTCCCT TTGGCAAACT TATAGAGGAT 
CTGCAGCGGA TGCAGCCGAC CAATCCGCAG TCGGCCCTGG TGCCAATGGG TCCAGTTGCG 
TCGGCTTCGA TTCCTCCTCA ATACGGCTTT CCACCTCATC AGCAACGTCC AACGGCTGCT 
CAGCCCACAC CGTACGGCAT GGTTGCAGGT GGAGTTGTTG GTGGACCGGC TTACGGTGAC 
CTGCAGTTGG TGCCTTACCA ACCAGCTGCT CAGCAACAGA GGCCACTAAA CAGCGAGGAG 
CTGCAGCGGC TGTACAGCAT GCCCGCTCAA ATGGCCGTGG TTCCAGTGCC GCAGCCAAAC 
GCCTATATGT ACTATCCCGG AGCTGTGGTT ACTCCATACA CGGCTCCCAT TGTTCCCGGA 
TCGGCTGCTT TTATGCCGCC GCAGTATCCC GCACAGGGAT ATGGCTTTGG AGGTGCTTAC 
ACGCACATGG ATTTGCGTCG ACCCCAATCG CAACCAGCTC CCCAACAAAC AGCACCGACA 
ACAAGTCATC ATCACAGCCA ACCGTCCAAC CATTCCACTT CCTCCCCCGC AGAGGCCAAT 
GGAGTAGCCT TCCCAGCGCG TCGCCAAGTG CCCTCGACTG TCGGGGTTAG CTCTAGTAGC 
CACACTGGAA ACAATGGTCA TTCCTCGGTC CCACGCAGGG GCAACGATTT GATCGACCTC 
AACCACGAGG ACTACTCCCG TGTGAGTGTG CTGGAGGCAT TCGATCCCCT GCTAAACGAC 
AATACTGTGT CGAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261724.1
CDS	507..1607
Translation	MSNQAHIDYDKQFQDDLAKATALSLEQHALDDYRRNKKYGSGYQQSSTVAGRDYQAAQRSQSLHQPRRHSEVHQVAISPENAERSRTPPAQGTDNDLICFASPTSKQPESSSPFGKLIEDLQRMQPTNPQSALVPMGPVASASIPPQYGFPPHQQRPTAAQPTPYGMVAGGVVGGPAYGDLQLVPYQPAAQQQRPLNSEELQRLYSMPAQMAVVPVPQPNAYMYYPGAVVTPYTAPIVPGSAAFMPPQYPAQGYGFGGAYTHMDLRRPQSQPAPQQTAPTTSHHHSQPSNHSTSSPAEANGVAFPARRQVPSTVGVSSSSHTGNNGHSSVPRRGNDLIDLNHEDYSRVSVLEAFDPLLNDNTVSKK

Target ORF information:

RefSeq Version	NM_001274795.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphotidylinositol 3 kinase 68D, transcript variant E (Pi3K68D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274795.1

ORF Insert Sequence:

ATGTCAAATC AAGCGCATAT CGACTACGAC AAACAATTCC AGGATGACCT GGCCAAGGCG 
ACCGCCCTGA GTCTAGAGCA GCATGCCCTC GATGACTACA GGCGAAACAA GAAGTACGGC 
TCCGGGTATC AGCAAAGCTC CACCGTTGCT GGCCGAGATT ACCAGGCGGC GCAACGTAGT 
CAGAGCCTAC ATCAACCACG ACGGCACTCG GAGGTGCATC AGGTGGCCAT CAGTCCGGAG 
AATGCGGAAC GATCGCGCAC ACCGCCGGCC CAGGGAACGG ATAACGATCT GATCTGCTTC 
GCAAGTCCCA CCAGCAAGCA GCCAGAGAGT AGCAGTCCCT TTGGCAAACT TATAGAGGAT 
CTGCAGCGGA TGCAGCCGAC CAATCCGCAG TCGGCCCTGG TGCCAATGGG TCCAGTTGCG 
TCGGCTTCGA TTCCTCCTCA ATACGGCTTT CCACCTCATC AGCAACGTCC AACGGCTGCT 
CAGCCCACAC CGTACGGCAT GGTTGCAGGT GGAGTTGTTG GTGGACCGGC TTACGGTGAC 
CTGCAGTTGG TGCCTTACCA ACCAGCTGCT CAGCAACAGA GGCCACTAAA CAGCGAGGAG 
CTGCAGCGGC TGTACAGCAT GCCCGCTCAA ATGGCCGTGG TTCCAGTGCC GCAGCCAAAC 
GCCTATATGT ACTATCCCGG AGCTGTGGTT ACTCCATACA CGGCTCCCAT TGTTCCCGGA 
TCGGCTGCTT TTATGCCGCC GCAGTATCCC GCACAGGGAT ATGGCTTTGG AGGTGCTTAC 
ACGCACATGG ATTTGCGTCG ACCCCAATCG CAACCAGCTC CCCAACAAAC AGCACCGACA 
ACAAGTCATC ATCACAGCCA ACCGTCCAAC CATTCCACTT CCTCCCCCGC AGAGGCCAAT 
GGAGTAGCCT TCCCAGCGCG TCGCCAAGTG CCCTCGACTG TCGGGGTTAG CTCTAGTAGC 
CACACTGGAA ACAATGGTCA TTCCTCGGTC CCACGCAGGG GCAACGATTT GATCGACCTC 
AACCACGAGG ACTACTCCCG TGTGAGTGTG CTGGAGGCAT TCGATCCCCT GCTAAACGAC 
AATACTGTGT CGAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pi3K68D ( NM_168475.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729743.1 Drosophila melanogaster phosphotidylinositol 3 kinase 68D, transcript variant C (Pi3K68D), mRNA.

Price & Availability↑

CloneID	OFa18284
Clone ID Related Accession (Same CDS sequence)	NM_168475.3 , NM_001169949.2 , NM_079304.4
Accession Version	NM_168475.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphotidylinositol 3 kinase 68D, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168475.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAAATC AAGCGCATAT CGACTACGAC AAACAATTCC AGGATGACCT GGCCAAGGCG 
ACCGCCCTGA GTCTAGAGCA GCATGCCCTC GATGACTACA GGCGAAACAA GAAGTACGGC 
TCCGGGTATC AGCAAAGCTC CACCGTTGCT GGCCGAGATT ACCAGGCGGC GCAACGTAGT 
CAGAGCCTAC ATCAACCACG ACGGCACTCG GAGGTGCATC AGGTGGCCAT CAGTCCGGAG 
AATGCGGAAC GATCGCGCAC ACCGCCGGCC CAGGGAACGG ATAACGATCT GATCTGCTTC 
GCAAGTCCCA CCAGCAAGCA GCCAGAGAGT AGCAGTCCCT TTGGCAAACT TATAGAGGAT 
CTGCAGCGGA TGCAGCCGAC CAATCCGCAG TCGGCCCTGG TGCCAATGGG TCCAGTTGCG 
TCGGCTTCGA TTCCTCCTCA ATACGGCTTT CCACCTCATC AGCAACGTCC AACGGCTGCT 
CAGCCCACAC CGTACGGCAT GGTTGCAGGT GGAGTTGTTG GTGGACCGGC TTACGGTGAC 
CTGCAGTTGG TGCCTTACCA ACCAGCTGCT CAGCAACAGA GGCCACTAAA CAGCGAGGAG 
CTGCAGCGGC TGTACAGCAT GCCCGCTCAA ATGGCCGTGG TTCCAGTGCC GCAGCCAAAC 
GCCTATATGT ACTATCCCGG AGCTGTGGTT ACTCCATACA CGGCTCCCAT TGTTCCCGGA 
TCGGCTGCTT TTATGCCGCC GCAGTATCCC GCACAGGGAT ATGGCTTTGG AGGTGCTTAC 
ACGCACATGG ATTTGCGTCG ACCCCAATCG CAACCAGCTC CCCAACAAAC AGCACCGACA 
ACAAGTCATC ATCACAGCCA ACCGTCCAAC CATTCCACTT CCTCCCCCGC AGAGGCCAAT 
GGAGTAGCCT TCCCAGCGCG TCGCCAAGTG CCCTCGACTG TCGGGGTTAG CTCTAGTAGC 
CACACTGGAA ACAATGGTCA TTCCTCGGTC CCACGCAGGG GCAACGATTT GATCGACCTC 
AACCACGAGG ACTACTCCCG TGTGAGTGTG CTGGAGGCAT TCGATCCCCT GCTAAACGAC 
AATACTGGCA ACGACACCGC CTCCGACAGC ACTTCCTACT ATGCGGAATA CGATCCCTTT 
GATTTTCTGT ACAGCGGAGA TGCAGCAACC CAATATTCCG ATCCAATGTA TGAGGCAGTC 
AACAGGTGGG ACAAAACTGT GGCCACCGTG AGTCCGAATG TTGGTCTAAT CGGTTGGCGC 
CAAGATTTTC TGAGCCAGCC ATCTACATCT TCATCGCAAT ATGGTGTTGC GCCGCCAGAG 
GAGAGTCTGA AGCTTGCGGA GAACGGATCT GGAACTATCT CGCCTCCTCC GCCGTTGCCG 
CCCCGGAACC AGCAGTGCTA TGAATCAAAC CAGGCAGCCA TGCCGGTCTC CAGGCCTCCT 
CAGTCTTCTG TTTTGACGGA CAGCTACACC TCCAGCATTC CGGCCAACGT GGTGCTGGAC 
CGGCGGAAAA CTTGTACACG ACTGTACGAA TTGATCAGCG ACCAGCGCAC TGATGATCCC 
GAACTTTTGG AATTTTACCA CATGGTAAAG GAGGTGAGGG CACGCTATCC GCATGACGAT 
GCGCCCACCA ATGTGGGACA TGTTGTGGCC GCCGAGTTTA ATTATCACTA CATGATGGAC 
ACCAGCATCA AAGTGATTGT GCATCCGGCT CTAAATACAC TTCAATCAAC GGTCCTGGCT 
GCGTCCATGG GCAAGGAACA GGTGAAGGGA TATGGAATGC CAGTAACATT CACTTGCGAT 
ATTGATTCGG TTGTGGCACA GGTGGTGGCA CAAGCTTTGG CCTCGCTGGA GGGACAAGTC 
AAGGGTACCG TCACAGATTA TGCGGTCAAG CCCATTGGTC TTCTGGAGTG GCTGGCACCC 
ACCTCGAGAC TGAGTCAGCT GGAGTGCGTG CACAATAGCT TCCAATTGGA GAAGGATGTA 
CATTTGGGCC TGTGTCTTAG TACGGCGGCA AACATGCAGG CTATTGCACG AACTGAGCGG 
GATGATGAGC ACGATGCGGA TTTGCTGCCG GAACATCTTC TTCCCAACGA GGTGGTGCCA 
ATTGTGACCT ACGACAATAT GATGATACTC ATCGAAACGC TGGAGATGGA GATTGACAAG 
CTGGAATCGG CGGCCGACGG AGTACCCGGA CGGAGTGTCG TGAGCTGCTC CGGAGTTGTC 
CAAGCAGTGA AGGCCATATG CGCACTGCTC GGTTCAATCG ACACAATGGA AATTGCACGA 
TGTGTTGCCG ATCTGAAGCG CATTTGCGAG GTGGAGCAAA AGAAGTACTC GACGGGCGCT 
AGCAACCCAG AGATTGTGAG TGACTATGGT GATTACGCTC AAGTTGTACT CCGCCCGCGC 
TCCATGCTGG AGCAGATCAA GGTCAAGTGC AACGAGCTGC GAGATGCAGT GCAAGAGCTG 
GTTGAATTGT ATGCGAATGT TTTCCGGGTG GCATTCTCCG TGAAGACGCC CGATTACTCA 
ACAACACCCA TACCCATTTC CTGCGTGTCC AAACCAATTG TGGTATGCAT TAGCTGCCTA 
CACAGGCCGC TGCCGAATTG GAAGTTCGAC GATTATTCCC TGTGCGTACA AATCGTTTAT 
GGAACGCGCC TGCTGTCGAA GCCGAATGTG CTGACCTGCT CCAACGATAC AAGTGGAGGC 
CTGTTTCCTC GTCTTAACTT CAGTGCCTGG CTGACTTTCG ATCAGCATCC CATCTGCACT 
CTGCCCAGGG AGGCGCGCCT TACGTTCGTG TTGTATGGAA AACAGGCGGC CAGCGAAGGC 
GAACCCAACG CCGATCAGAA TGGAGAGAGG CGTCAGGTGA CCACTGAACT GGGTTGGTGT 
TCGATCCAAC TGTTTGACTT TAAGCGAGTG ATGATCTGCG GCCCCTACTT ACTGTCTTTA 
TGGCCACCAA TGACGGACAA AATGCTTGGA CCAGCTCCGG CTCGAGGCTG TCATCCGCAA 
CCCGACTTTT GCCCCGTTTT GAGCATTGAA GTACCTCCGT ATGGAGGACG CATTGAGTTT 
CCTGAGCACC AGGAGGTGCC AAAACCTGCA CCACACTACG ATTTTGCCTC TCTGGATGCC 
AATCTTCAAG AGGAGCTGCT GGACACCGCA GAGCTGGGCT ACACAGGAGC CACAGAACGA 
CGTGAAGTGT TCTGGGAAAA ACGGCTCTAC CTGCAGAGCT ATCCCAATGC CCTGCCAAAG 
GTTCTTCATG CCGCTCACAG TTGGGATTAT GCCAATTTGA TCGATTTGCA TGCGCTGCTG 
CACTCCTGGG CACCACTCTC GCCATTGCAG TCGTTGGAGT TACTTCTGCC ACGATATCCG 
GATGCTAAGG TTCGCGAGAA AGCCGTGGAG TGGATCTCCA AGATGCCCAA CGACCAGCTC 
GTCGACTTTC TGCCTCAATT GGTGCAAAGT TTAAAACATG ACACATACGA AGGCTCGGCA 
ATGGCTCGAT TCTTGCTGTC CAAATGCCTG GAGTCACCGC GCTTTGCCCA TCACATGTAT 
TGGCTGCTTG TACACAGTCT GCCTGACGAT CCCCACAACT CTATTGGAGC AGCGATGGTG 
GATCAGGAGT ATGACGAGTC TCAGGTTACC CAGGTCCGTT ACTACCGCCG GAACAAAATG 
ATGCTGCGTG CTTTAATGGC GATTTGCGGC GAAAAGATGC TTCAGCGATT TATGTACCAG 
CACCGAATGT GTCAGAAACT TACTACTATT GCGGAGTCGG TTAAAGAGGC TAAGGAGTCG 
ATGCGTCAAA AAAGCCTAGC CGCAGGCATG GACGAGGTGC ACCAAGACTT ACTGGAGCAA 
CCCACTTGCC TACCGCTGGG ACCAGAACTG GAGGTAACTG GAGTGAGTGT GCGTAACTGT 
AGCTACTTTA ACTCCAACAC GCTGCCGCTG AAGATCAACT TTGTGGGACC TGATGCCGAA 
TCTTTACCGG CTATCTTTAA GTGCGGAGAT GACTTGCAGC AGGATCAGTT AACTATACAG 
CTAATTAGGA TTATGAACAA AATGTGGTTG GCCGAACGAT TGGACCTGAA GATGGTCACC 
TTTAATTGTG TGCCTACGGG ATACAAGAGC GGTATGATTG AGCTGGTTAG CGAGGCGGAA 
ACGTTGAGAA AAATTCAAGT AGAGTGCGGT CTGACGGGGT CCTTTAAGGA TCGCCCGATC 
GCTGAGTGGT TAGGCAAGCA GAATCCCAGT CCTCTCGAGT ACCAGAGTGC TGTGCGAAAT 
TTTACGCTAT CCTGTGCTGG ATACAGTGTG GCCACGTATG TGCTAGGCAT CTGTGATCGC 
CACAATGACA ACATCATGTT AAAGACTTCG GGTCACTTGT TTCACATTGA CTTTGGCAAG 
TTTCTTGGCG ATGCTCAGAT GTTTGGAAAC TTTAAGAGAG ATCGCACTCC ATTTGTCCTG 
ACTTCCGACA TGGCTTATGT CATAAATGGC GGCGATAAGC CCTCCACAGA CTTTCACTAT 
TTCGTGGACC TATGTTGTCG AGCCTTTAAT ATCGTGCGGA AAAATGCTGA TCTACTCTTG 
CACACCCTGG CCCACATGGC TACAGCAGGC ATGCCGGGAG TAAACTCCAA TGCTGTGCAA 
TATGTACGAC GCGCCCTATT GCCATCTCAA TCGAATCCCG AGGCAGCTGC CACATTTGCC 
AAGATGATTC AATCCTCTTT GAAAAGCTGG TTCACGCAAT TCAATTTCTT TCTGCACAAT 
CTGGCCCAGA TGCGTTTCAC CCCAGACGAG GGATCAGGAG AGCTGCTATC GTTCGTGCCA 
CGAAAATATA CAATGCAGCA GGATGGTCGC TTGAAGATTG TAAAGGTGGT GTGTTTCCAG 
AAGCATTACA GCATGGAAAA GTTTTATATG TATATTCTGG AAGTGACGCG ACATGGACAG 
CCCGATCCGA CACATTTGTT CCGGTCATAT CGGGAATTCA CGGAATTCCA TCAGAAGTTA 
TGCATGCACT TTCCTTTGGT TAAACTGCAC AGTCTGCCGG CTGGTGTGCA TGTGGGCCGT 
TCCAATATCA AATCCGTGGC AGAAAAACGA CTACCTCTTA TTCAGCGATT TTTGAAATCG 
TTGTTCGATG CGTCCGAGGA AATAGCCCAT TCCGAGCTCG TTTACACATT CTTTCACCCG 
CTGCTGCGCG ATCAGCAGGA AGCCAAGCTT GGGATGCCGA AGATAAAGGA GGTGAAGCAA 
CAACCGTCGC GGGATAATCC CCACGAGATT GGCCAAATAC GACTATCGCT GCAATATCAA 
CGCGGCGTAC TTACTGTGAT GATACACCAC GCCAAAGGAC TGCCCATGTT ACAGGGCGGT 
CAGGAGCCCA ACACATATGT GAAGTGCTAC CTAAAACCGG ATCCCAAAAA GGAGACCAAA 
CGCAAGACCA AAGTGGTGCG CAAGACCTGT GTGCCCAGTT TCATGGAAAC TTTGGAGTAC 
CGAATGCCAC TGAATATTAT TCAAGAGCGC CGCCTTCAGG TTACGGTTTG GTCGCACGAC 
ACCCTGCAGG AGAACGAGCT GCTTGGAGGC TTCGATATGG ATCTGTCGAA GTACGACCTG 
CGACAGGAGC TCGTCGACTG GTATCGCCTG GGCGCGGTGT CCAGGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729743.1
CDS	206..5836
Translation	MSNQAHIDYDKQFQDDLAKATALSLEQHALDDYRRNKKYGSGYQQSSTVAGRDYQAAQRSQSLHQPRRHSEVHQVAISPENAERSRTPPAQGTDNDLICFASPTSKQPESSSPFGKLIEDLQRMQPTNPQSALVPMGPVASASIPPQYGFPPHQQRPTAAQPTPYGMVAGGVVGGPAYGDLQLVPYQPAAQQQRPLNSEELQRLYSMPAQMAVVPVPQPNAYMYYPGAVVTPYTAPIVPGSAAFMPPQYPAQGYGFGGAYTHMDLRRPQSQPAPQQTAPTTSHHHSQPSNHSTSSPAEANGVAFPARRQVPSTVGVSSSSHTGNNGHSSVPRRGNDLIDLNHEDYSRVSVLEAFDPLLNDNTGNDTASDSTSYYAEYDPFDFLYSGDAATQYSDPMYEAVNRWDKTVATVSPNVGLIGWRQDFLSQPSTSSSQYGVAPPEESLKLAENGSGTISPPPPLPPRNQQCYESNQAAMPVSRPPQSSVLTDSYTSSIPANVVLDRRKTCTRLYELISDQRTDDPELLEFYHMVKEVRARYPHDDAPTNVGHVVAAEFNYHYMMDTSIKVIVHPALNTLQSTVLAASMGKEQVKGYGMPVTFTCDIDSVVAQVVAQALASLEGQVKGTVTDYAVKPIGLLEWLAPTSRLSQLECVHNSFQLEKDVHLGLCLSTAANMQAIARTERDDEHDADLLPEHLLPNEVVPIVTYDNMMILIETLEMEIDKLESAADGVPGRSVVSCSGVVQAVKAICALLGSIDTMEIARCVADLKRICEVEQKKYSTGASNPEIVSDYGDYAQVVLRPRSMLEQIKVKCNELRDAVQELVELYANVFRVAFSVKTPDYSTTPIPISCVSKPIVVCISCLHRPLPNWKFDDYSLCVQIVYGTRLLSKPNVLTCSNDTSGGLFPRLNFSAWLTFDQHPICTLPREARLTFVLYGKQAASEGEPNADQNGERRQVTTELGWCSIQLFDFKRVMICGPYLLSLWPPMTDKMLGPAPARGCHPQPDFCPVLSIEVPPYGGRIEFPEHQEVPKPAPHYDFASLDANLQEELLDTAELGYTGATERREVFWEKRLYLQSYPNALPKVLHAAHSWDYANLIDLHALLHSWAPLSPLQSLELLLPRYPDAKVREKAVEWISKMPNDQLVDFLPQLVQSLKHDTYEGSAMARFLLSKCLESPRFAHHMYWLLVHSLPDDPHNSIGAAMVDQEYDESQVTQVRYYRRNKMMLRALMAICGEKMLQRFMYQHRMCQKLTTIAESVKEAKESMRQKSLAAGMDEVHQDLLEQPTCLPLGPELEVTGVSVRNCSYFNSNTLPLKINFVGPDAESLPAIFKCGDDLQQDQLTIQLIRIMNKMWLAERLDLKMVTFNCVPTGYKSGMIELVSEAETLRKIQVECGLTGSFKDRPIAEWLGKQNPSPLEYQSAVRNFTLSCAGYSVATYVLGICDRHNDNIMLKTSGHLFHIDFGKFLGDAQMFGNFKRDRTPFVLTSDMAYVINGGDKPSTDFHYFVDLCCRAFNIVRKNADLLLHTLAHMATAGMPGVNSNAVQYVRRALLPSQSNPEAAATFAKMIQSSLKSWFTQFNFFLHNLAQMRFTPDEGSGELLSFVPRKYTMQQDGRLKIVKVVCFQKHYSMEKFYMYILEVTRHGQPDPTHLFRSYREFTEFHQKLCMHFPLVKLHSLPAGVHVGRSNIKSVAEKRLPLIQRFLKSLFDASEEIAHSELVYTFFHPLLRDQQEAKLGMPKIKEVKQQPSRDNPHEIGQIRLSLQYQRGVLTVMIHHAKGLPMLQGGQEPNTYVKCYLKPDPKKETKRKTKVVRKTCVPSFMETLEYRMPLNIIQERRLQVTVWSHDTLQENELLGGFDMDLSKYDLRQELVDWYRLGAVSRN

Target ORF information:

RefSeq Version	NM_168475.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphotidylinositol 3 kinase 68D, transcript variant C (Pi3K68D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168475.3

ORF Insert Sequence:

ATGTCAAATC AAGCGCATAT CGACTACGAC AAACAATTCC AGGATGACCT GGCCAAGGCG 
ACCGCCCTGA GTCTAGAGCA GCATGCCCTC GATGACTACA GGCGAAACAA GAAGTACGGC 
TCCGGGTATC AGCAAAGCTC CACCGTTGCT GGCCGAGATT ACCAGGCGGC GCAACGTAGT 
CAGAGCCTAC ATCAACCACG ACGGCACTCG GAGGTGCATC AGGTGGCCAT CAGTCCGGAG 
AATGCGGAAC GATCGCGCAC ACCGCCGGCC CAGGGAACGG ATAACGATCT GATCTGCTTC 
GCAAGTCCCA CCAGCAAGCA GCCAGAGAGT AGCAGTCCCT TTGGCAAACT TATAGAGGAT 
CTGCAGCGGA TGCAGCCGAC CAATCCGCAG TCGGCCCTGG TGCCAATGGG TCCAGTTGCG 
TCGGCTTCGA TTCCTCCTCA ATACGGCTTT CCACCTCATC AGCAACGTCC AACGGCTGCT 
CAGCCCACAC CGTACGGCAT GGTTGCAGGT GGAGTTGTTG GTGGACCGGC TTACGGTGAC 
CTGCAGTTGG TGCCTTACCA ACCAGCTGCT CAGCAACAGA GGCCACTAAA CAGCGAGGAG 
CTGCAGCGGC TGTACAGCAT GCCCGCTCAA ATGGCCGTGG TTCCAGTGCC GCAGCCAAAC 
GCCTATATGT ACTATCCCGG AGCTGTGGTT ACTCCATACA CGGCTCCCAT TGTTCCCGGA 
TCGGCTGCTT TTATGCCGCC GCAGTATCCC GCACAGGGAT ATGGCTTTGG AGGTGCTTAC 
ACGCACATGG ATTTGCGTCG ACCCCAATCG CAACCAGCTC CCCAACAAAC AGCACCGACA 
ACAAGTCATC ATCACAGCCA ACCGTCCAAC CATTCCACTT CCTCCCCCGC AGAGGCCAAT 
GGAGTAGCCT TCCCAGCGCG TCGCCAAGTG CCCTCGACTG TCGGGGTTAG CTCTAGTAGC 
CACACTGGAA ACAATGGTCA TTCCTCGGTC CCACGCAGGG GCAACGATTT GATCGACCTC 
AACCACGAGG ACTACTCCCG TGTGAGTGTG CTGGAGGCAT TCGATCCCCT GCTAAACGAC 
AATACTGGCA ACGACACCGC CTCCGACAGC ACTTCCTACT ATGCGGAATA CGATCCCTTT 
GATTTTCTGT ACAGCGGAGA TGCAGCAACC CAATATTCCG ATCCAATGTA TGAGGCAGTC 
AACAGGTGGG ACAAAACTGT GGCCACCGTG AGTCCGAATG TTGGTCTAAT CGGTTGGCGC 
CAAGATTTTC TGAGCCAGCC ATCTACATCT TCATCGCAAT ATGGTGTTGC GCCGCCAGAG 
GAGAGTCTGA AGCTTGCGGA GAACGGATCT GGAACTATCT CGCCTCCTCC GCCGTTGCCG 
CCCCGGAACC AGCAGTGCTA TGAATCAAAC CAGGCAGCCA TGCCGGTCTC CAGGCCTCCT 
CAGTCTTCTG TTTTGACGGA CAGCTACACC TCCAGCATTC CGGCCAACGT GGTGCTGGAC 
CGGCGGAAAA CTTGTACACG ACTGTACGAA TTGATCAGCG ACCAGCGCAC TGATGATCCC 
GAACTTTTGG AATTTTACCA CATGGTAAAG GAGGTGAGGG CACGCTATCC GCATGACGAT 
GCGCCCACCA ATGTGGGACA TGTTGTGGCC GCCGAGTTTA ATTATCACTA CATGATGGAC 
ACCAGCATCA AAGTGATTGT GCATCCGGCT CTAAATACAC TTCAATCAAC GGTCCTGGCT 
GCGTCCATGG GCAAGGAACA GGTGAAGGGA TATGGAATGC CAGTAACATT CACTTGCGAT 
ATTGATTCGG TTGTGGCACA GGTGGTGGCA CAAGCTTTGG CCTCGCTGGA GGGACAAGTC 
AAGGGTACCG TCACAGATTA TGCGGTCAAG CCCATTGGTC TTCTGGAGTG GCTGGCACCC 
ACCTCGAGAC TGAGTCAGCT GGAGTGCGTG CACAATAGCT TCCAATTGGA GAAGGATGTA 
CATTTGGGCC TGTGTCTTAG TACGGCGGCA AACATGCAGG CTATTGCACG AACTGAGCGG 
GATGATGAGC ACGATGCGGA TTTGCTGCCG GAACATCTTC TTCCCAACGA GGTGGTGCCA 
ATTGTGACCT ACGACAATAT GATGATACTC ATCGAAACGC TGGAGATGGA GATTGACAAG 
CTGGAATCGG CGGCCGACGG AGTACCCGGA CGGAGTGTCG TGAGCTGCTC CGGAGTTGTC 
CAAGCAGTGA AGGCCATATG CGCACTGCTC GGTTCAATCG ACACAATGGA AATTGCACGA 
TGTGTTGCCG ATCTGAAGCG CATTTGCGAG GTGGAGCAAA AGAAGTACTC GACGGGCGCT 
AGCAACCCAG AGATTGTGAG TGACTATGGT GATTACGCTC AAGTTGTACT CCGCCCGCGC 
TCCATGCTGG AGCAGATCAA GGTCAAGTGC AACGAGCTGC GAGATGCAGT GCAAGAGCTG 
GTTGAATTGT ATGCGAATGT TTTCCGGGTG GCATTCTCCG TGAAGACGCC CGATTACTCA 
ACAACACCCA TACCCATTTC CTGCGTGTCC AAACCAATTG TGGTATGCAT TAGCTGCCTA 
CACAGGCCGC TGCCGAATTG GAAGTTCGAC GATTATTCCC TGTGCGTACA AATCGTTTAT 
GGAACGCGCC TGCTGTCGAA GCCGAATGTG CTGACCTGCT CCAACGATAC AAGTGGAGGC 
CTGTTTCCTC GTCTTAACTT CAGTGCCTGG CTGACTTTCG ATCAGCATCC CATCTGCACT 
CTGCCCAGGG AGGCGCGCCT TACGTTCGTG TTGTATGGAA AACAGGCGGC CAGCGAAGGC 
GAACCCAACG CCGATCAGAA TGGAGAGAGG CGTCAGGTGA CCACTGAACT GGGTTGGTGT 
TCGATCCAAC TGTTTGACTT TAAGCGAGTG ATGATCTGCG GCCCCTACTT ACTGTCTTTA 
TGGCCACCAA TGACGGACAA AATGCTTGGA CCAGCTCCGG CTCGAGGCTG TCATCCGCAA 
CCCGACTTTT GCCCCGTTTT GAGCATTGAA GTACCTCCGT ATGGAGGACG CATTGAGTTT 
CCTGAGCACC AGGAGGTGCC AAAACCTGCA CCACACTACG ATTTTGCCTC TCTGGATGCC 
AATCTTCAAG AGGAGCTGCT GGACACCGCA GAGCTGGGCT ACACAGGAGC CACAGAACGA 
CGTGAAGTGT TCTGGGAAAA ACGGCTCTAC CTGCAGAGCT ATCCCAATGC CCTGCCAAAG 
GTTCTTCATG CCGCTCACAG TTGGGATTAT GCCAATTTGA TCGATTTGCA TGCGCTGCTG 
CACTCCTGGG CACCACTCTC GCCATTGCAG TCGTTGGAGT TACTTCTGCC ACGATATCCG 
GATGCTAAGG TTCGCGAGAA AGCCGTGGAG TGGATCTCCA AGATGCCCAA CGACCAGCTC 
GTCGACTTTC TGCCTCAATT GGTGCAAAGT TTAAAACATG ACACATACGA AGGCTCGGCA 
ATGGCTCGAT TCTTGCTGTC CAAATGCCTG GAGTCACCGC GCTTTGCCCA TCACATGTAT 
TGGCTGCTTG TACACAGTCT GCCTGACGAT CCCCACAACT CTATTGGAGC AGCGATGGTG 
GATCAGGAGT ATGACGAGTC TCAGGTTACC CAGGTCCGTT ACTACCGCCG GAACAAAATG 
ATGCTGCGTG CTTTAATGGC GATTTGCGGC GAAAAGATGC TTCAGCGATT TATGTACCAG 
CACCGAATGT GTCAGAAACT TACTACTATT GCGGAGTCGG TTAAAGAGGC TAAGGAGTCG 
ATGCGTCAAA AAAGCCTAGC CGCAGGCATG GACGAGGTGC ACCAAGACTT ACTGGAGCAA 
CCCACTTGCC TACCGCTGGG ACCAGAACTG GAGGTAACTG GAGTGAGTGT GCGTAACTGT 
AGCTACTTTA ACTCCAACAC GCTGCCGCTG AAGATCAACT TTGTGGGACC TGATGCCGAA 
TCTTTACCGG CTATCTTTAA GTGCGGAGAT GACTTGCAGC AGGATCAGTT AACTATACAG 
CTAATTAGGA TTATGAACAA AATGTGGTTG GCCGAACGAT TGGACCTGAA GATGGTCACC 
TTTAATTGTG TGCCTACGGG ATACAAGAGC GGTATGATTG AGCTGGTTAG CGAGGCGGAA 
ACGTTGAGAA AAATTCAAGT AGAGTGCGGT CTGACGGGGT CCTTTAAGGA TCGCCCGATC 
GCTGAGTGGT TAGGCAAGCA GAATCCCAGT CCTCTCGAGT ACCAGAGTGC TGTGCGAAAT 
TTTACGCTAT CCTGTGCTGG ATACAGTGTG GCCACGTATG TGCTAGGCAT CTGTGATCGC 
CACAATGACA ACATCATGTT AAAGACTTCG GGTCACTTGT TTCACATTGA CTTTGGCAAG 
TTTCTTGGCG ATGCTCAGAT GTTTGGAAAC TTTAAGAGAG ATCGCACTCC ATTTGTCCTG 
ACTTCCGACA TGGCTTATGT CATAAATGGC GGCGATAAGC CCTCCACAGA CTTTCACTAT 
TTCGTGGACC TATGTTGTCG AGCCTTTAAT ATCGTGCGGA AAAATGCTGA TCTACTCTTG 
CACACCCTGG CCCACATGGC TACAGCAGGC ATGCCGGGAG TAAACTCCAA TGCTGTGCAA 
TATGTACGAC GCGCCCTATT GCCATCTCAA TCGAATCCCG AGGCAGCTGC CACATTTGCC 
AAGATGATTC AATCCTCTTT GAAAAGCTGG TTCACGCAAT TCAATTTCTT TCTGCACAAT 
CTGGCCCAGA TGCGTTTCAC CCCAGACGAG GGATCAGGAG AGCTGCTATC GTTCGTGCCA 
CGAAAATATA CAATGCAGCA GGATGGTCGC TTGAAGATTG TAAAGGTGGT GTGTTTCCAG 
AAGCATTACA GCATGGAAAA GTTTTATATG TATATTCTGG AAGTGACGCG ACATGGACAG 
CCCGATCCGA CACATTTGTT CCGGTCATAT CGGGAATTCA CGGAATTCCA TCAGAAGTTA 
TGCATGCACT TTCCTTTGGT TAAACTGCAC AGTCTGCCGG CTGGTGTGCA TGTGGGCCGT 
TCCAATATCA AATCCGTGGC AGAAAAACGA CTACCTCTTA TTCAGCGATT TTTGAAATCG 
TTGTTCGATG CGTCCGAGGA AATAGCCCAT TCCGAGCTCG TTTACACATT CTTTCACCCG 
CTGCTGCGCG ATCAGCAGGA AGCCAAGCTT GGGATGCCGA AGATAAAGGA GGTGAAGCAA 
CAACCGTCGC GGGATAATCC CCACGAGATT GGCCAAATAC GACTATCGCT GCAATATCAA 
CGCGGCGTAC TTACTGTGAT GATACACCAC GCCAAAGGAC TGCCCATGTT ACAGGGCGGT 
CAGGAGCCCA ACACATATGT GAAGTGCTAC CTAAAACCGG ATCCCAAAAA GGAGACCAAA 
CGCAAGACCA AAGTGGTGCG CAAGACCTGT GTGCCCAGTT TCATGGAAAC TTTGGAGTAC 
CGAATGCCAC TGAATATTAT TCAAGAGCGC CGCCTTCAGG TTACGGTTTG GTCGCACGAC 
ACCCTGCAGG AGAACGAGCT GCTTGGAGGC TTCGATATGG ATCTGTCGAA GTACGACCTG 
CGACAGGAGC TCGTCGACTG GTATCGCCTG GGCGCGGTGT CCAGGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pi3K68D ( NM_001169949.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163420.1 Drosophila melanogaster phosphotidylinositol 3 kinase 68D, transcript variant D (Pi3K68D), mRNA.

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CloneID	OFa18284
Clone ID Related Accession (Same CDS sequence)	NM_168475.3 , NM_001169949.2 , NM_079304.4
Accession Version	NM_001169949.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphotidylinositol 3 kinase 68D, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001169949.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAAATC AAGCGCATAT CGACTACGAC AAACAATTCC AGGATGACCT GGCCAAGGCG 
ACCGCCCTGA GTCTAGAGCA GCATGCCCTC GATGACTACA GGCGAAACAA GAAGTACGGC 
TCCGGGTATC AGCAAAGCTC CACCGTTGCT GGCCGAGATT ACCAGGCGGC GCAACGTAGT 
CAGAGCCTAC ATCAACCACG ACGGCACTCG GAGGTGCATC AGGTGGCCAT CAGTCCGGAG 
AATGCGGAAC GATCGCGCAC ACCGCCGGCC CAGGGAACGG ATAACGATCT GATCTGCTTC 
GCAAGTCCCA CCAGCAAGCA GCCAGAGAGT AGCAGTCCCT TTGGCAAACT TATAGAGGAT 
CTGCAGCGGA TGCAGCCGAC CAATCCGCAG TCGGCCCTGG TGCCAATGGG TCCAGTTGCG 
TCGGCTTCGA TTCCTCCTCA ATACGGCTTT CCACCTCATC AGCAACGTCC AACGGCTGCT 
CAGCCCACAC CGTACGGCAT GGTTGCAGGT GGAGTTGTTG GTGGACCGGC TTACGGTGAC 
CTGCAGTTGG TGCCTTACCA ACCAGCTGCT CAGCAACAGA GGCCACTAAA CAGCGAGGAG 
CTGCAGCGGC TGTACAGCAT GCCCGCTCAA ATGGCCGTGG TTCCAGTGCC GCAGCCAAAC 
GCCTATATGT ACTATCCCGG AGCTGTGGTT ACTCCATACA CGGCTCCCAT TGTTCCCGGA 
TCGGCTGCTT TTATGCCGCC GCAGTATCCC GCACAGGGAT ATGGCTTTGG AGGTGCTTAC 
ACGCACATGG ATTTGCGTCG ACCCCAATCG CAACCAGCTC CCCAACAAAC AGCACCGACA 
ACAAGTCATC ATCACAGCCA ACCGTCCAAC CATTCCACTT CCTCCCCCGC AGAGGCCAAT 
GGAGTAGCCT TCCCAGCGCG TCGCCAAGTG CCCTCGACTG TCGGGGTTAG CTCTAGTAGC 
CACACTGGAA ACAATGGTCA TTCCTCGGTC CCACGCAGGG GCAACGATTT GATCGACCTC 
AACCACGAGG ACTACTCCCG TGTGAGTGTG CTGGAGGCAT TCGATCCCCT GCTAAACGAC 
AATACTGGCA ACGACACCGC CTCCGACAGC ACTTCCTACT ATGCGGAATA CGATCCCTTT 
GATTTTCTGT ACAGCGGAGA TGCAGCAACC CAATATTCCG ATCCAATGTA TGAGGCAGTC 
AACAGGTGGG ACAAAACTGT GGCCACCGTG AGTCCGAATG TTGGTCTAAT CGGTTGGCGC 
CAAGATTTTC TGAGCCAGCC ATCTACATCT TCATCGCAAT ATGGTGTTGC GCCGCCAGAG 
GAGAGTCTGA AGCTTGCGGA GAACGGATCT GGAACTATCT CGCCTCCTCC GCCGTTGCCG 
CCCCGGAACC AGCAGTGCTA TGAATCAAAC CAGGCAGCCA TGCCGGTCTC CAGGCCTCCT 
CAGTCTTCTG TTTTGACGGA CAGCTACACC TCCAGCATTC CGGCCAACGT GGTGCTGGAC 
CGGCGGAAAA CTTGTACACG ACTGTACGAA TTGATCAGCG ACCAGCGCAC TGATGATCCC 
GAACTTTTGG AATTTTACCA CATGGTAAAG GAGGTGAGGG CACGCTATCC GCATGACGAT 
GCGCCCACCA ATGTGGGACA TGTTGTGGCC GCCGAGTTTA ATTATCACTA CATGATGGAC 
ACCAGCATCA AAGTGATTGT GCATCCGGCT CTAAATACAC TTCAATCAAC GGTCCTGGCT 
GCGTCCATGG GCAAGGAACA GGTGAAGGGA TATGGAATGC CAGTAACATT CACTTGCGAT 
ATTGATTCGG TTGTGGCACA GGTGGTGGCA CAAGCTTTGG CCTCGCTGGA GGGACAAGTC 
AAGGGTACCG TCACAGATTA TGCGGTCAAG CCCATTGGTC TTCTGGAGTG GCTGGCACCC 
ACCTCGAGAC TGAGTCAGCT GGAGTGCGTG CACAATAGCT TCCAATTGGA GAAGGATGTA 
CATTTGGGCC TGTGTCTTAG TACGGCGGCA AACATGCAGG CTATTGCACG AACTGAGCGG 
GATGATGAGC ACGATGCGGA TTTGCTGCCG GAACATCTTC TTCCCAACGA GGTGGTGCCA 
ATTGTGACCT ACGACAATAT GATGATACTC ATCGAAACGC TGGAGATGGA GATTGACAAG 
CTGGAATCGG CGGCCGACGG AGTACCCGGA CGGAGTGTCG TGAGCTGCTC CGGAGTTGTC 
CAAGCAGTGA AGGCCATATG CGCACTGCTC GGTTCAATCG ACACAATGGA AATTGCACGA 
TGTGTTGCCG ATCTGAAGCG CATTTGCGAG GTGGAGCAAA AGAAGTACTC GACGGGCGCT 
AGCAACCCAG AGATTGTGAG TGACTATGGT GATTACGCTC AAGTTGTACT CCGCCCGCGC 
TCCATGCTGG AGCAGATCAA GGTCAAGTGC AACGAGCTGC GAGATGCAGT GCAAGAGCTG 
GTTGAATTGT ATGCGAATGT TTTCCGGGTG GCATTCTCCG TGAAGACGCC CGATTACTCA 
ACAACACCCA TACCCATTTC CTGCGTGTCC AAACCAATTG TGGTATGCAT TAGCTGCCTA 
CACAGGCCGC TGCCGAATTG GAAGTTCGAC GATTATTCCC TGTGCGTACA AATCGTTTAT 
GGAACGCGCC TGCTGTCGAA GCCGAATGTG CTGACCTGCT CCAACGATAC AAGTGGAGGC 
CTGTTTCCTC GTCTTAACTT CAGTGCCTGG CTGACTTTCG ATCAGCATCC CATCTGCACT 
CTGCCCAGGG AGGCGCGCCT TACGTTCGTG TTGTATGGAA AACAGGCGGC CAGCGAAGGC 
GAACCCAACG CCGATCAGAA TGGAGAGAGG CGTCAGGTGA CCACTGAACT GGGTTGGTGT 
TCGATCCAAC TGTTTGACTT TAAGCGAGTG ATGATCTGCG GCCCCTACTT ACTGTCTTTA 
TGGCCACCAA TGACGGACAA AATGCTTGGA CCAGCTCCGG CTCGAGGCTG TCATCCGCAA 
CCCGACTTTT GCCCCGTTTT GAGCATTGAA GTACCTCCGT ATGGAGGACG CATTGAGTTT 
CCTGAGCACC AGGAGGTGCC AAAACCTGCA CCACACTACG ATTTTGCCTC TCTGGATGCC 
AATCTTCAAG AGGAGCTGCT GGACACCGCA GAGCTGGGCT ACACAGGAGC CACAGAACGA 
CGTGAAGTGT TCTGGGAAAA ACGGCTCTAC CTGCAGAGCT ATCCCAATGC CCTGCCAAAG 
GTTCTTCATG CCGCTCACAG TTGGGATTAT GCCAATTTGA TCGATTTGCA TGCGCTGCTG 
CACTCCTGGG CACCACTCTC GCCATTGCAG TCGTTGGAGT TACTTCTGCC ACGATATCCG 
GATGCTAAGG TTCGCGAGAA AGCCGTGGAG TGGATCTCCA AGATGCCCAA CGACCAGCTC 
GTCGACTTTC TGCCTCAATT GGTGCAAAGT TTAAAACATG ACACATACGA AGGCTCGGCA 
ATGGCTCGAT TCTTGCTGTC CAAATGCCTG GAGTCACCGC GCTTTGCCCA TCACATGTAT 
TGGCTGCTTG TACACAGTCT GCCTGACGAT CCCCACAACT CTATTGGAGC AGCGATGGTG 
GATCAGGAGT ATGACGAGTC TCAGGTTACC CAGGTCCGTT ACTACCGCCG GAACAAAATG 
ATGCTGCGTG CTTTAATGGC GATTTGCGGC GAAAAGATGC TTCAGCGATT TATGTACCAG 
CACCGAATGT GTCAGAAACT TACTACTATT GCGGAGTCGG TTAAAGAGGC TAAGGAGTCG 
ATGCGTCAAA AAAGCCTAGC CGCAGGCATG GACGAGGTGC ACCAAGACTT ACTGGAGCAA 
CCCACTTGCC TACCGCTGGG ACCAGAACTG GAGGTAACTG GAGTGAGTGT GCGTAACTGT 
AGCTACTTTA ACTCCAACAC GCTGCCGCTG AAGATCAACT TTGTGGGACC TGATGCCGAA 
TCTTTACCGG CTATCTTTAA GTGCGGAGAT GACTTGCAGC AGGATCAGTT AACTATACAG 
CTAATTAGGA TTATGAACAA AATGTGGTTG GCCGAACGAT TGGACCTGAA GATGGTCACC 
TTTAATTGTG TGCCTACGGG ATACAAGAGC GGTATGATTG AGCTGGTTAG CGAGGCGGAA 
ACGTTGAGAA AAATTCAAGT AGAGTGCGGT CTGACGGGGT CCTTTAAGGA TCGCCCGATC 
GCTGAGTGGT TAGGCAAGCA GAATCCCAGT CCTCTCGAGT ACCAGAGTGC TGTGCGAAAT 
TTTACGCTAT CCTGTGCTGG ATACAGTGTG GCCACGTATG TGCTAGGCAT CTGTGATCGC 
CACAATGACA ACATCATGTT AAAGACTTCG GGTCACTTGT TTCACATTGA CTTTGGCAAG 
TTTCTTGGCG ATGCTCAGAT GTTTGGAAAC TTTAAGAGAG ATCGCACTCC ATTTGTCCTG 
ACTTCCGACA TGGCTTATGT CATAAATGGC GGCGATAAGC CCTCCACAGA CTTTCACTAT 
TTCGTGGACC TATGTTGTCG AGCCTTTAAT ATCGTGCGGA AAAATGCTGA TCTACTCTTG 
CACACCCTGG CCCACATGGC TACAGCAGGC ATGCCGGGAG TAAACTCCAA TGCTGTGCAA 
TATGTACGAC GCGCCCTATT GCCATCTCAA TCGAATCCCG AGGCAGCTGC CACATTTGCC 
AAGATGATTC AATCCTCTTT GAAAAGCTGG TTCACGCAAT TCAATTTCTT TCTGCACAAT 
CTGGCCCAGA TGCGTTTCAC CCCAGACGAG GGATCAGGAG AGCTGCTATC GTTCGTGCCA 
CGAAAATATA CAATGCAGCA GGATGGTCGC TTGAAGATTG TAAAGGTGGT GTGTTTCCAG 
AAGCATTACA GCATGGAAAA GTTTTATATG TATATTCTGG AAGTGACGCG ACATGGACAG 
CCCGATCCGA CACATTTGTT CCGGTCATAT CGGGAATTCA CGGAATTCCA TCAGAAGTTA 
TGCATGCACT TTCCTTTGGT TAAACTGCAC AGTCTGCCGG CTGGTGTGCA TGTGGGCCGT 
TCCAATATCA AATCCGTGGC AGAAAAACGA CTACCTCTTA TTCAGCGATT TTTGAAATCG 
TTGTTCGATG CGTCCGAGGA AATAGCCCAT TCCGAGCTCG TTTACACATT CTTTCACCCG 
CTGCTGCGCG ATCAGCAGGA AGCCAAGCTT GGGATGCCGA AGATAAAGGA GGTGAAGCAA 
CAACCGTCGC GGGATAATCC CCACGAGATT GGCCAAATAC GACTATCGCT GCAATATCAA 
CGCGGCGTAC TTACTGTGAT GATACACCAC GCCAAAGGAC TGCCCATGTT ACAGGGCGGT 
CAGGAGCCCA ACACATATGT GAAGTGCTAC CTAAAACCGG ATCCCAAAAA GGAGACCAAA 
CGCAAGACCA AAGTGGTGCG CAAGACCTGT GTGCCCAGTT TCATGGAAAC TTTGGAGTAC 
CGAATGCCAC TGAATATTAT TCAAGAGCGC CGCCTTCAGG TTACGGTTTG GTCGCACGAC 
ACCCTGCAGG AGAACGAGCT GCTTGGAGGC TTCGATATGG ATCTGTCGAA GTACGACCTG 
CGACAGGAGC TCGTCGACTG GTATCGCCTG GGCGCGGTGT CCAGGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163420.1
CDS	158..5788
Translation	MSNQAHIDYDKQFQDDLAKATALSLEQHALDDYRRNKKYGSGYQQSSTVAGRDYQAAQRSQSLHQPRRHSEVHQVAISPENAERSRTPPAQGTDNDLICFASPTSKQPESSSPFGKLIEDLQRMQPTNPQSALVPMGPVASASIPPQYGFPPHQQRPTAAQPTPYGMVAGGVVGGPAYGDLQLVPYQPAAQQQRPLNSEELQRLYSMPAQMAVVPVPQPNAYMYYPGAVVTPYTAPIVPGSAAFMPPQYPAQGYGFGGAYTHMDLRRPQSQPAPQQTAPTTSHHHSQPSNHSTSSPAEANGVAFPARRQVPSTVGVSSSSHTGNNGHSSVPRRGNDLIDLNHEDYSRVSVLEAFDPLLNDNTGNDTASDSTSYYAEYDPFDFLYSGDAATQYSDPMYEAVNRWDKTVATVSPNVGLIGWRQDFLSQPSTSSSQYGVAPPEESLKLAENGSGTISPPPPLPPRNQQCYESNQAAMPVSRPPQSSVLTDSYTSSIPANVVLDRRKTCTRLYELISDQRTDDPELLEFYHMVKEVRARYPHDDAPTNVGHVVAAEFNYHYMMDTSIKVIVHPALNTLQSTVLAASMGKEQVKGYGMPVTFTCDIDSVVAQVVAQALASLEGQVKGTVTDYAVKPIGLLEWLAPTSRLSQLECVHNSFQLEKDVHLGLCLSTAANMQAIARTERDDEHDADLLPEHLLPNEVVPIVTYDNMMILIETLEMEIDKLESAADGVPGRSVVSCSGVVQAVKAICALLGSIDTMEIARCVADLKRICEVEQKKYSTGASNPEIVSDYGDYAQVVLRPRSMLEQIKVKCNELRDAVQELVELYANVFRVAFSVKTPDYSTTPIPISCVSKPIVVCISCLHRPLPNWKFDDYSLCVQIVYGTRLLSKPNVLTCSNDTSGGLFPRLNFSAWLTFDQHPICTLPREARLTFVLYGKQAASEGEPNADQNGERRQVTTELGWCSIQLFDFKRVMICGPYLLSLWPPMTDKMLGPAPARGCHPQPDFCPVLSIEVPPYGGRIEFPEHQEVPKPAPHYDFASLDANLQEELLDTAELGYTGATERREVFWEKRLYLQSYPNALPKVLHAAHSWDYANLIDLHALLHSWAPLSPLQSLELLLPRYPDAKVREKAVEWISKMPNDQLVDFLPQLVQSLKHDTYEGSAMARFLLSKCLESPRFAHHMYWLLVHSLPDDPHNSIGAAMVDQEYDESQVTQVRYYRRNKMMLRALMAICGEKMLQRFMYQHRMCQKLTTIAESVKEAKESMRQKSLAAGMDEVHQDLLEQPTCLPLGPELEVTGVSVRNCSYFNSNTLPLKINFVGPDAESLPAIFKCGDDLQQDQLTIQLIRIMNKMWLAERLDLKMVTFNCVPTGYKSGMIELVSEAETLRKIQVECGLTGSFKDRPIAEWLGKQNPSPLEYQSAVRNFTLSCAGYSVATYVLGICDRHNDNIMLKTSGHLFHIDFGKFLGDAQMFGNFKRDRTPFVLTSDMAYVINGGDKPSTDFHYFVDLCCRAFNIVRKNADLLLHTLAHMATAGMPGVNSNAVQYVRRALLPSQSNPEAAATFAKMIQSSLKSWFTQFNFFLHNLAQMRFTPDEGSGELLSFVPRKYTMQQDGRLKIVKVVCFQKHYSMEKFYMYILEVTRHGQPDPTHLFRSYREFTEFHQKLCMHFPLVKLHSLPAGVHVGRSNIKSVAEKRLPLIQRFLKSLFDASEEIAHSELVYTFFHPLLRDQQEAKLGMPKIKEVKQQPSRDNPHEIGQIRLSLQYQRGVLTVMIHHAKGLPMLQGGQEPNTYVKCYLKPDPKKETKRKTKVVRKTCVPSFMETLEYRMPLNIIQERRLQVTVWSHDTLQENELLGGFDMDLSKYDLRQELVDWYRLGAVSRN

Target ORF information:

RefSeq Version	NM_001169949.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphotidylinositol 3 kinase 68D, transcript variant D (Pi3K68D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169949.2

ORF Insert Sequence:

ATGTCAAATC AAGCGCATAT CGACTACGAC AAACAATTCC AGGATGACCT GGCCAAGGCG 
ACCGCCCTGA GTCTAGAGCA GCATGCCCTC GATGACTACA GGCGAAACAA GAAGTACGGC 
TCCGGGTATC AGCAAAGCTC CACCGTTGCT GGCCGAGATT ACCAGGCGGC GCAACGTAGT 
CAGAGCCTAC ATCAACCACG ACGGCACTCG GAGGTGCATC AGGTGGCCAT CAGTCCGGAG 
AATGCGGAAC GATCGCGCAC ACCGCCGGCC CAGGGAACGG ATAACGATCT GATCTGCTTC 
GCAAGTCCCA CCAGCAAGCA GCCAGAGAGT AGCAGTCCCT TTGGCAAACT TATAGAGGAT 
CTGCAGCGGA TGCAGCCGAC CAATCCGCAG TCGGCCCTGG TGCCAATGGG TCCAGTTGCG 
TCGGCTTCGA TTCCTCCTCA ATACGGCTTT CCACCTCATC AGCAACGTCC AACGGCTGCT 
CAGCCCACAC CGTACGGCAT GGTTGCAGGT GGAGTTGTTG GTGGACCGGC TTACGGTGAC 
CTGCAGTTGG TGCCTTACCA ACCAGCTGCT CAGCAACAGA GGCCACTAAA CAGCGAGGAG 
CTGCAGCGGC TGTACAGCAT GCCCGCTCAA ATGGCCGTGG TTCCAGTGCC GCAGCCAAAC 
GCCTATATGT ACTATCCCGG AGCTGTGGTT ACTCCATACA CGGCTCCCAT TGTTCCCGGA 
TCGGCTGCTT TTATGCCGCC GCAGTATCCC GCACAGGGAT ATGGCTTTGG AGGTGCTTAC 
ACGCACATGG ATTTGCGTCG ACCCCAATCG CAACCAGCTC CCCAACAAAC AGCACCGACA 
ACAAGTCATC ATCACAGCCA ACCGTCCAAC CATTCCACTT CCTCCCCCGC AGAGGCCAAT 
GGAGTAGCCT TCCCAGCGCG TCGCCAAGTG CCCTCGACTG TCGGGGTTAG CTCTAGTAGC 
CACACTGGAA ACAATGGTCA TTCCTCGGTC CCACGCAGGG GCAACGATTT GATCGACCTC 
AACCACGAGG ACTACTCCCG TGTGAGTGTG CTGGAGGCAT TCGATCCCCT GCTAAACGAC 
AATACTGGCA ACGACACCGC CTCCGACAGC ACTTCCTACT ATGCGGAATA CGATCCCTTT 
GATTTTCTGT ACAGCGGAGA TGCAGCAACC CAATATTCCG ATCCAATGTA TGAGGCAGTC 
AACAGGTGGG ACAAAACTGT GGCCACCGTG AGTCCGAATG TTGGTCTAAT CGGTTGGCGC 
CAAGATTTTC TGAGCCAGCC ATCTACATCT TCATCGCAAT ATGGTGTTGC GCCGCCAGAG 
GAGAGTCTGA AGCTTGCGGA GAACGGATCT GGAACTATCT CGCCTCCTCC GCCGTTGCCG 
CCCCGGAACC AGCAGTGCTA TGAATCAAAC CAGGCAGCCA TGCCGGTCTC CAGGCCTCCT 
CAGTCTTCTG TTTTGACGGA CAGCTACACC TCCAGCATTC CGGCCAACGT GGTGCTGGAC 
CGGCGGAAAA CTTGTACACG ACTGTACGAA TTGATCAGCG ACCAGCGCAC TGATGATCCC 
GAACTTTTGG AATTTTACCA CATGGTAAAG GAGGTGAGGG CACGCTATCC GCATGACGAT 
GCGCCCACCA ATGTGGGACA TGTTGTGGCC GCCGAGTTTA ATTATCACTA CATGATGGAC 
ACCAGCATCA AAGTGATTGT GCATCCGGCT CTAAATACAC TTCAATCAAC GGTCCTGGCT 
GCGTCCATGG GCAAGGAACA GGTGAAGGGA TATGGAATGC CAGTAACATT CACTTGCGAT 
ATTGATTCGG TTGTGGCACA GGTGGTGGCA CAAGCTTTGG CCTCGCTGGA GGGACAAGTC 
AAGGGTACCG TCACAGATTA TGCGGTCAAG CCCATTGGTC TTCTGGAGTG GCTGGCACCC 
ACCTCGAGAC TGAGTCAGCT GGAGTGCGTG CACAATAGCT TCCAATTGGA GAAGGATGTA 
CATTTGGGCC TGTGTCTTAG TACGGCGGCA AACATGCAGG CTATTGCACG AACTGAGCGG 
GATGATGAGC ACGATGCGGA TTTGCTGCCG GAACATCTTC TTCCCAACGA GGTGGTGCCA 
ATTGTGACCT ACGACAATAT GATGATACTC ATCGAAACGC TGGAGATGGA GATTGACAAG 
CTGGAATCGG CGGCCGACGG AGTACCCGGA CGGAGTGTCG TGAGCTGCTC CGGAGTTGTC 
CAAGCAGTGA AGGCCATATG CGCACTGCTC GGTTCAATCG ACACAATGGA AATTGCACGA 
TGTGTTGCCG ATCTGAAGCG CATTTGCGAG GTGGAGCAAA AGAAGTACTC GACGGGCGCT 
AGCAACCCAG AGATTGTGAG TGACTATGGT GATTACGCTC AAGTTGTACT CCGCCCGCGC 
TCCATGCTGG AGCAGATCAA GGTCAAGTGC AACGAGCTGC GAGATGCAGT GCAAGAGCTG 
GTTGAATTGT ATGCGAATGT TTTCCGGGTG GCATTCTCCG TGAAGACGCC CGATTACTCA 
ACAACACCCA TACCCATTTC CTGCGTGTCC AAACCAATTG TGGTATGCAT TAGCTGCCTA 
CACAGGCCGC TGCCGAATTG GAAGTTCGAC GATTATTCCC TGTGCGTACA AATCGTTTAT 
GGAACGCGCC TGCTGTCGAA GCCGAATGTG CTGACCTGCT CCAACGATAC AAGTGGAGGC 
CTGTTTCCTC GTCTTAACTT CAGTGCCTGG CTGACTTTCG ATCAGCATCC CATCTGCACT 
CTGCCCAGGG AGGCGCGCCT TACGTTCGTG TTGTATGGAA AACAGGCGGC CAGCGAAGGC 
GAACCCAACG CCGATCAGAA TGGAGAGAGG CGTCAGGTGA CCACTGAACT GGGTTGGTGT 
TCGATCCAAC TGTTTGACTT TAAGCGAGTG ATGATCTGCG GCCCCTACTT ACTGTCTTTA 
TGGCCACCAA TGACGGACAA AATGCTTGGA CCAGCTCCGG CTCGAGGCTG TCATCCGCAA 
CCCGACTTTT GCCCCGTTTT GAGCATTGAA GTACCTCCGT ATGGAGGACG CATTGAGTTT 
CCTGAGCACC AGGAGGTGCC AAAACCTGCA CCACACTACG ATTTTGCCTC TCTGGATGCC 
AATCTTCAAG AGGAGCTGCT GGACACCGCA GAGCTGGGCT ACACAGGAGC CACAGAACGA 
CGTGAAGTGT TCTGGGAAAA ACGGCTCTAC CTGCAGAGCT ATCCCAATGC CCTGCCAAAG 
GTTCTTCATG CCGCTCACAG TTGGGATTAT GCCAATTTGA TCGATTTGCA TGCGCTGCTG 
CACTCCTGGG CACCACTCTC GCCATTGCAG TCGTTGGAGT TACTTCTGCC ACGATATCCG 
GATGCTAAGG TTCGCGAGAA AGCCGTGGAG TGGATCTCCA AGATGCCCAA CGACCAGCTC 
GTCGACTTTC TGCCTCAATT GGTGCAAAGT TTAAAACATG ACACATACGA AGGCTCGGCA 
ATGGCTCGAT TCTTGCTGTC CAAATGCCTG GAGTCACCGC GCTTTGCCCA TCACATGTAT 
TGGCTGCTTG TACACAGTCT GCCTGACGAT CCCCACAACT CTATTGGAGC AGCGATGGTG 
GATCAGGAGT ATGACGAGTC TCAGGTTACC CAGGTCCGTT ACTACCGCCG GAACAAAATG 
ATGCTGCGTG CTTTAATGGC GATTTGCGGC GAAAAGATGC TTCAGCGATT TATGTACCAG 
CACCGAATGT GTCAGAAACT TACTACTATT GCGGAGTCGG TTAAAGAGGC TAAGGAGTCG 
ATGCGTCAAA AAAGCCTAGC CGCAGGCATG GACGAGGTGC ACCAAGACTT ACTGGAGCAA 
CCCACTTGCC TACCGCTGGG ACCAGAACTG GAGGTAACTG GAGTGAGTGT GCGTAACTGT 
AGCTACTTTA ACTCCAACAC GCTGCCGCTG AAGATCAACT TTGTGGGACC TGATGCCGAA 
TCTTTACCGG CTATCTTTAA GTGCGGAGAT GACTTGCAGC AGGATCAGTT AACTATACAG 
CTAATTAGGA TTATGAACAA AATGTGGTTG GCCGAACGAT TGGACCTGAA GATGGTCACC 
TTTAATTGTG TGCCTACGGG ATACAAGAGC GGTATGATTG AGCTGGTTAG CGAGGCGGAA 
ACGTTGAGAA AAATTCAAGT AGAGTGCGGT CTGACGGGGT CCTTTAAGGA TCGCCCGATC 
GCTGAGTGGT TAGGCAAGCA GAATCCCAGT CCTCTCGAGT ACCAGAGTGC TGTGCGAAAT 
TTTACGCTAT CCTGTGCTGG ATACAGTGTG GCCACGTATG TGCTAGGCAT CTGTGATCGC 
CACAATGACA ACATCATGTT AAAGACTTCG GGTCACTTGT TTCACATTGA CTTTGGCAAG 
TTTCTTGGCG ATGCTCAGAT GTTTGGAAAC TTTAAGAGAG ATCGCACTCC ATTTGTCCTG 
ACTTCCGACA TGGCTTATGT CATAAATGGC GGCGATAAGC CCTCCACAGA CTTTCACTAT 
TTCGTGGACC TATGTTGTCG AGCCTTTAAT ATCGTGCGGA AAAATGCTGA TCTACTCTTG 
CACACCCTGG CCCACATGGC TACAGCAGGC ATGCCGGGAG TAAACTCCAA TGCTGTGCAA 
TATGTACGAC GCGCCCTATT GCCATCTCAA TCGAATCCCG AGGCAGCTGC CACATTTGCC 
AAGATGATTC AATCCTCTTT GAAAAGCTGG TTCACGCAAT TCAATTTCTT TCTGCACAAT 
CTGGCCCAGA TGCGTTTCAC CCCAGACGAG GGATCAGGAG AGCTGCTATC GTTCGTGCCA 
CGAAAATATA CAATGCAGCA GGATGGTCGC TTGAAGATTG TAAAGGTGGT GTGTTTCCAG 
AAGCATTACA GCATGGAAAA GTTTTATATG TATATTCTGG AAGTGACGCG ACATGGACAG 
CCCGATCCGA CACATTTGTT CCGGTCATAT CGGGAATTCA CGGAATTCCA TCAGAAGTTA 
TGCATGCACT TTCCTTTGGT TAAACTGCAC AGTCTGCCGG CTGGTGTGCA TGTGGGCCGT 
TCCAATATCA AATCCGTGGC AGAAAAACGA CTACCTCTTA TTCAGCGATT TTTGAAATCG 
TTGTTCGATG CGTCCGAGGA AATAGCCCAT TCCGAGCTCG TTTACACATT CTTTCACCCG 
CTGCTGCGCG ATCAGCAGGA AGCCAAGCTT GGGATGCCGA AGATAAAGGA GGTGAAGCAA 
CAACCGTCGC GGGATAATCC CCACGAGATT GGCCAAATAC GACTATCGCT GCAATATCAA 
CGCGGCGTAC TTACTGTGAT GATACACCAC GCCAAAGGAC TGCCCATGTT ACAGGGCGGT 
CAGGAGCCCA ACACATATGT GAAGTGCTAC CTAAAACCGG ATCCCAAAAA GGAGACCAAA 
CGCAAGACCA AAGTGGTGCG CAAGACCTGT GTGCCCAGTT TCATGGAAAC TTTGGAGTAC 
CGAATGCCAC TGAATATTAT TCAAGAGCGC CGCCTTCAGG TTACGGTTTG GTCGCACGAC 
ACCCTGCAGG AGAACGAGCT GCTTGGAGGC TTCGATATGG ATCTGTCGAA GTACGACCTG 
CGACAGGAGC TCGTCGACTG GTATCGCCTG GGCGCGGTGT CCAGGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pi3K68D ( NM_079304.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524028.2 Drosophila melanogaster phosphotidylinositol 3 kinase 68D, transcript variant A (Pi3K68D), mRNA.

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CloneID	OFa18284
Clone ID Related Accession (Same CDS sequence)	NM_168475.3 , NM_001169949.2 , NM_079304.4
Accession Version	NM_079304.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphotidylinositol 3 kinase 68D, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079304.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAAATC AAGCGCATAT CGACTACGAC AAACAATTCC AGGATGACCT GGCCAAGGCG 
ACCGCCCTGA GTCTAGAGCA GCATGCCCTC GATGACTACA GGCGAAACAA GAAGTACGGC 
TCCGGGTATC AGCAAAGCTC CACCGTTGCT GGCCGAGATT ACCAGGCGGC GCAACGTAGT 
CAGAGCCTAC ATCAACCACG ACGGCACTCG GAGGTGCATC AGGTGGCCAT CAGTCCGGAG 
AATGCGGAAC GATCGCGCAC ACCGCCGGCC CAGGGAACGG ATAACGATCT GATCTGCTTC 
GCAAGTCCCA CCAGCAAGCA GCCAGAGAGT AGCAGTCCCT TTGGCAAACT TATAGAGGAT 
CTGCAGCGGA TGCAGCCGAC CAATCCGCAG TCGGCCCTGG TGCCAATGGG TCCAGTTGCG 
TCGGCTTCGA TTCCTCCTCA ATACGGCTTT CCACCTCATC AGCAACGTCC AACGGCTGCT 
CAGCCCACAC CGTACGGCAT GGTTGCAGGT GGAGTTGTTG GTGGACCGGC TTACGGTGAC 
CTGCAGTTGG TGCCTTACCA ACCAGCTGCT CAGCAACAGA GGCCACTAAA CAGCGAGGAG 
CTGCAGCGGC TGTACAGCAT GCCCGCTCAA ATGGCCGTGG TTCCAGTGCC GCAGCCAAAC 
GCCTATATGT ACTATCCCGG AGCTGTGGTT ACTCCATACA CGGCTCCCAT TGTTCCCGGA 
TCGGCTGCTT TTATGCCGCC GCAGTATCCC GCACAGGGAT ATGGCTTTGG AGGTGCTTAC 
ACGCACATGG ATTTGCGTCG ACCCCAATCG CAACCAGCTC CCCAACAAAC AGCACCGACA 
ACAAGTCATC ATCACAGCCA ACCGTCCAAC CATTCCACTT CCTCCCCCGC AGAGGCCAAT 
GGAGTAGCCT TCCCAGCGCG TCGCCAAGTG CCCTCGACTG TCGGGGTTAG CTCTAGTAGC 
CACACTGGAA ACAATGGTCA TTCCTCGGTC CCACGCAGGG GCAACGATTT GATCGACCTC 
AACCACGAGG ACTACTCCCG TGTGAGTGTG CTGGAGGCAT TCGATCCCCT GCTAAACGAC 
AATACTGGCA ACGACACCGC CTCCGACAGC ACTTCCTACT ATGCGGAATA CGATCCCTTT 
GATTTTCTGT ACAGCGGAGA TGCAGCAACC CAATATTCCG ATCCAATGTA TGAGGCAGTC 
AACAGGTGGG ACAAAACTGT GGCCACCGTG AGTCCGAATG TTGGTCTAAT CGGTTGGCGC 
CAAGATTTTC TGAGCCAGCC ATCTACATCT TCATCGCAAT ATGGTGTTGC GCCGCCAGAG 
GAGAGTCTGA AGCTTGCGGA GAACGGATCT GGAACTATCT CGCCTCCTCC GCCGTTGCCG 
CCCCGGAACC AGCAGTGCTA TGAATCAAAC CAGGCAGCCA TGCCGGTCTC CAGGCCTCCT 
CAGTCTTCTG TTTTGACGGA CAGCTACACC TCCAGCATTC CGGCCAACGT GGTGCTGGAC 
CGGCGGAAAA CTTGTACACG ACTGTACGAA TTGATCAGCG ACCAGCGCAC TGATGATCCC 
GAACTTTTGG AATTTTACCA CATGGTAAAG GAGGTGAGGG CACGCTATCC GCATGACGAT 
GCGCCCACCA ATGTGGGACA TGTTGTGGCC GCCGAGTTTA ATTATCACTA CATGATGGAC 
ACCAGCATCA AAGTGATTGT GCATCCGGCT CTAAATACAC TTCAATCAAC GGTCCTGGCT 
GCGTCCATGG GCAAGGAACA GGTGAAGGGA TATGGAATGC CAGTAACATT CACTTGCGAT 
ATTGATTCGG TTGTGGCACA GGTGGTGGCA CAAGCTTTGG CCTCGCTGGA GGGACAAGTC 
AAGGGTACCG TCACAGATTA TGCGGTCAAG CCCATTGGTC TTCTGGAGTG GCTGGCACCC 
ACCTCGAGAC TGAGTCAGCT GGAGTGCGTG CACAATAGCT TCCAATTGGA GAAGGATGTA 
CATTTGGGCC TGTGTCTTAG TACGGCGGCA AACATGCAGG CTATTGCACG AACTGAGCGG 
GATGATGAGC ACGATGCGGA TTTGCTGCCG GAACATCTTC TTCCCAACGA GGTGGTGCCA 
ATTGTGACCT ACGACAATAT GATGATACTC ATCGAAACGC TGGAGATGGA GATTGACAAG 
CTGGAATCGG CGGCCGACGG AGTACCCGGA CGGAGTGTCG TGAGCTGCTC CGGAGTTGTC 
CAAGCAGTGA AGGCCATATG CGCACTGCTC GGTTCAATCG ACACAATGGA AATTGCACGA 
TGTGTTGCCG ATCTGAAGCG CATTTGCGAG GTGGAGCAAA AGAAGTACTC GACGGGCGCT 
AGCAACCCAG AGATTGTGAG TGACTATGGT GATTACGCTC AAGTTGTACT CCGCCCGCGC 
TCCATGCTGG AGCAGATCAA GGTCAAGTGC AACGAGCTGC GAGATGCAGT GCAAGAGCTG 
GTTGAATTGT ATGCGAATGT TTTCCGGGTG GCATTCTCCG TGAAGACGCC CGATTACTCA 
ACAACACCCA TACCCATTTC CTGCGTGTCC AAACCAATTG TGGTATGCAT TAGCTGCCTA 
CACAGGCCGC TGCCGAATTG GAAGTTCGAC GATTATTCCC TGTGCGTACA AATCGTTTAT 
GGAACGCGCC TGCTGTCGAA GCCGAATGTG CTGACCTGCT CCAACGATAC AAGTGGAGGC 
CTGTTTCCTC GTCTTAACTT CAGTGCCTGG CTGACTTTCG ATCAGCATCC CATCTGCACT 
CTGCCCAGGG AGGCGCGCCT TACGTTCGTG TTGTATGGAA AACAGGCGGC CAGCGAAGGC 
GAACCCAACG CCGATCAGAA TGGAGAGAGG CGTCAGGTGA CCACTGAACT GGGTTGGTGT 
TCGATCCAAC TGTTTGACTT TAAGCGAGTG ATGATCTGCG GCCCCTACTT ACTGTCTTTA 
TGGCCACCAA TGACGGACAA AATGCTTGGA CCAGCTCCGG CTCGAGGCTG TCATCCGCAA 
CCCGACTTTT GCCCCGTTTT GAGCATTGAA GTACCTCCGT ATGGAGGACG CATTGAGTTT 
CCTGAGCACC AGGAGGTGCC AAAACCTGCA CCACACTACG ATTTTGCCTC TCTGGATGCC 
AATCTTCAAG AGGAGCTGCT GGACACCGCA GAGCTGGGCT ACACAGGAGC CACAGAACGA 
CGTGAAGTGT TCTGGGAAAA ACGGCTCTAC CTGCAGAGCT ATCCCAATGC CCTGCCAAAG 
GTTCTTCATG CCGCTCACAG TTGGGATTAT GCCAATTTGA TCGATTTGCA TGCGCTGCTG 
CACTCCTGGG CACCACTCTC GCCATTGCAG TCGTTGGAGT TACTTCTGCC ACGATATCCG 
GATGCTAAGG TTCGCGAGAA AGCCGTGGAG TGGATCTCCA AGATGCCCAA CGACCAGCTC 
GTCGACTTTC TGCCTCAATT GGTGCAAAGT TTAAAACATG ACACATACGA AGGCTCGGCA 
ATGGCTCGAT TCTTGCTGTC CAAATGCCTG GAGTCACCGC GCTTTGCCCA TCACATGTAT 
TGGCTGCTTG TACACAGTCT GCCTGACGAT CCCCACAACT CTATTGGAGC AGCGATGGTG 
GATCAGGAGT ATGACGAGTC TCAGGTTACC CAGGTCCGTT ACTACCGCCG GAACAAAATG 
ATGCTGCGTG CTTTAATGGC GATTTGCGGC GAAAAGATGC TTCAGCGATT TATGTACCAG 
CACCGAATGT GTCAGAAACT TACTACTATT GCGGAGTCGG TTAAAGAGGC TAAGGAGTCG 
ATGCGTCAAA AAAGCCTAGC CGCAGGCATG GACGAGGTGC ACCAAGACTT ACTGGAGCAA 
CCCACTTGCC TACCGCTGGG ACCAGAACTG GAGGTAACTG GAGTGAGTGT GCGTAACTGT 
AGCTACTTTA ACTCCAACAC GCTGCCGCTG AAGATCAACT TTGTGGGACC TGATGCCGAA 
TCTTTACCGG CTATCTTTAA GTGCGGAGAT GACTTGCAGC AGGATCAGTT AACTATACAG 
CTAATTAGGA TTATGAACAA AATGTGGTTG GCCGAACGAT TGGACCTGAA GATGGTCACC 
TTTAATTGTG TGCCTACGGG ATACAAGAGC GGTATGATTG AGCTGGTTAG CGAGGCGGAA 
ACGTTGAGAA AAATTCAAGT AGAGTGCGGT CTGACGGGGT CCTTTAAGGA TCGCCCGATC 
GCTGAGTGGT TAGGCAAGCA GAATCCCAGT CCTCTCGAGT ACCAGAGTGC TGTGCGAAAT 
TTTACGCTAT CCTGTGCTGG ATACAGTGTG GCCACGTATG TGCTAGGCAT CTGTGATCGC 
CACAATGACA ACATCATGTT AAAGACTTCG GGTCACTTGT TTCACATTGA CTTTGGCAAG 
TTTCTTGGCG ATGCTCAGAT GTTTGGAAAC TTTAAGAGAG ATCGCACTCC ATTTGTCCTG 
ACTTCCGACA TGGCTTATGT CATAAATGGC GGCGATAAGC CCTCCACAGA CTTTCACTAT 
TTCGTGGACC TATGTTGTCG AGCCTTTAAT ATCGTGCGGA AAAATGCTGA TCTACTCTTG 
CACACCCTGG CCCACATGGC TACAGCAGGC ATGCCGGGAG TAAACTCCAA TGCTGTGCAA 
TATGTACGAC GCGCCCTATT GCCATCTCAA TCGAATCCCG AGGCAGCTGC CACATTTGCC 
AAGATGATTC AATCCTCTTT GAAAAGCTGG TTCACGCAAT TCAATTTCTT TCTGCACAAT 
CTGGCCCAGA TGCGTTTCAC CCCAGACGAG GGATCAGGAG AGCTGCTATC GTTCGTGCCA 
CGAAAATATA CAATGCAGCA GGATGGTCGC TTGAAGATTG TAAAGGTGGT GTGTTTCCAG 
AAGCATTACA GCATGGAAAA GTTTTATATG TATATTCTGG AAGTGACGCG ACATGGACAG 
CCCGATCCGA CACATTTGTT CCGGTCATAT CGGGAATTCA CGGAATTCCA TCAGAAGTTA 
TGCATGCACT TTCCTTTGGT TAAACTGCAC AGTCTGCCGG CTGGTGTGCA TGTGGGCCGT 
TCCAATATCA AATCCGTGGC AGAAAAACGA CTACCTCTTA TTCAGCGATT TTTGAAATCG 
TTGTTCGATG CGTCCGAGGA AATAGCCCAT TCCGAGCTCG TTTACACATT CTTTCACCCG 
CTGCTGCGCG ATCAGCAGGA AGCCAAGCTT GGGATGCCGA AGATAAAGGA GGTGAAGCAA 
CAACCGTCGC GGGATAATCC CCACGAGATT GGCCAAATAC GACTATCGCT GCAATATCAA 
CGCGGCGTAC TTACTGTGAT GATACACCAC GCCAAAGGAC TGCCCATGTT ACAGGGCGGT 
CAGGAGCCCA ACACATATGT GAAGTGCTAC CTAAAACCGG ATCCCAAAAA GGAGACCAAA 
CGCAAGACCA AAGTGGTGCG CAAGACCTGT GTGCCCAGTT TCATGGAAAC TTTGGAGTAC 
CGAATGCCAC TGAATATTAT TCAAGAGCGC CGCCTTCAGG TTACGGTTTG GTCGCACGAC 
ACCCTGCAGG AGAACGAGCT GCTTGGAGGC TTCGATATGG ATCTGTCGAA GTACGACCTG 
CGACAGGAGC TCGTCGACTG GTATCGCCTG GGCGCGGTGT CCAGGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524028.2
CDS	507..6137
Translation	MSNQAHIDYDKQFQDDLAKATALSLEQHALDDYRRNKKYGSGYQQSSTVAGRDYQAAQRSQSLHQPRRHSEVHQVAISPENAERSRTPPAQGTDNDLICFASPTSKQPESSSPFGKLIEDLQRMQPTNPQSALVPMGPVASASIPPQYGFPPHQQRPTAAQPTPYGMVAGGVVGGPAYGDLQLVPYQPAAQQQRPLNSEELQRLYSMPAQMAVVPVPQPNAYMYYPGAVVTPYTAPIVPGSAAFMPPQYPAQGYGFGGAYTHMDLRRPQSQPAPQQTAPTTSHHHSQPSNHSTSSPAEANGVAFPARRQVPSTVGVSSSSHTGNNGHSSVPRRGNDLIDLNHEDYSRVSVLEAFDPLLNDNTGNDTASDSTSYYAEYDPFDFLYSGDAATQYSDPMYEAVNRWDKTVATVSPNVGLIGWRQDFLSQPSTSSSQYGVAPPEESLKLAENGSGTISPPPPLPPRNQQCYESNQAAMPVSRPPQSSVLTDSYTSSIPANVVLDRRKTCTRLYELISDQRTDDPELLEFYHMVKEVRARYPHDDAPTNVGHVVAAEFNYHYMMDTSIKVIVHPALNTLQSTVLAASMGKEQVKGYGMPVTFTCDIDSVVAQVVAQALASLEGQVKGTVTDYAVKPIGLLEWLAPTSRLSQLECVHNSFQLEKDVHLGLCLSTAANMQAIARTERDDEHDADLLPEHLLPNEVVPIVTYDNMMILIETLEMEIDKLESAADGVPGRSVVSCSGVVQAVKAICALLGSIDTMEIARCVADLKRICEVEQKKYSTGASNPEIVSDYGDYAQVVLRPRSMLEQIKVKCNELRDAVQELVELYANVFRVAFSVKTPDYSTTPIPISCVSKPIVVCISCLHRPLPNWKFDDYSLCVQIVYGTRLLSKPNVLTCSNDTSGGLFPRLNFSAWLTFDQHPICTLPREARLTFVLYGKQAASEGEPNADQNGERRQVTTELGWCSIQLFDFKRVMICGPYLLSLWPPMTDKMLGPAPARGCHPQPDFCPVLSIEVPPYGGRIEFPEHQEVPKPAPHYDFASLDANLQEELLDTAELGYTGATERREVFWEKRLYLQSYPNALPKVLHAAHSWDYANLIDLHALLHSWAPLSPLQSLELLLPRYPDAKVREKAVEWISKMPNDQLVDFLPQLVQSLKHDTYEGSAMARFLLSKCLESPRFAHHMYWLLVHSLPDDPHNSIGAAMVDQEYDESQVTQVRYYRRNKMMLRALMAICGEKMLQRFMYQHRMCQKLTTIAESVKEAKESMRQKSLAAGMDEVHQDLLEQPTCLPLGPELEVTGVSVRNCSYFNSNTLPLKINFVGPDAESLPAIFKCGDDLQQDQLTIQLIRIMNKMWLAERLDLKMVTFNCVPTGYKSGMIELVSEAETLRKIQVECGLTGSFKDRPIAEWLGKQNPSPLEYQSAVRNFTLSCAGYSVATYVLGICDRHNDNIMLKTSGHLFHIDFGKFLGDAQMFGNFKRDRTPFVLTSDMAYVINGGDKPSTDFHYFVDLCCRAFNIVRKNADLLLHTLAHMATAGMPGVNSNAVQYVRRALLPSQSNPEAAATFAKMIQSSLKSWFTQFNFFLHNLAQMRFTPDEGSGELLSFVPRKYTMQQDGRLKIVKVVCFQKHYSMEKFYMYILEVTRHGQPDPTHLFRSYREFTEFHQKLCMHFPLVKLHSLPAGVHVGRSNIKSVAEKRLPLIQRFLKSLFDASEEIAHSELVYTFFHPLLRDQQEAKLGMPKIKEVKQQPSRDNPHEIGQIRLSLQYQRGVLTVMIHHAKGLPMLQGGQEPNTYVKCYLKPDPKKETKRKTKVVRKTCVPSFMETLEYRMPLNIIQERRLQVTVWSHDTLQENELLGGFDMDLSKYDLRQELVDWYRLGAVSRN

Target ORF information:

RefSeq Version	NM_079304.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphotidylinositol 3 kinase 68D, transcript variant A (Pi3K68D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079304.4

ORF Insert Sequence:

ATGTCAAATC AAGCGCATAT CGACTACGAC AAACAATTCC AGGATGACCT GGCCAAGGCG 
ACCGCCCTGA GTCTAGAGCA GCATGCCCTC GATGACTACA GGCGAAACAA GAAGTACGGC 
TCCGGGTATC AGCAAAGCTC CACCGTTGCT GGCCGAGATT ACCAGGCGGC GCAACGTAGT 
CAGAGCCTAC ATCAACCACG ACGGCACTCG GAGGTGCATC AGGTGGCCAT CAGTCCGGAG 
AATGCGGAAC GATCGCGCAC ACCGCCGGCC CAGGGAACGG ATAACGATCT GATCTGCTTC 
GCAAGTCCCA CCAGCAAGCA GCCAGAGAGT AGCAGTCCCT TTGGCAAACT TATAGAGGAT 
CTGCAGCGGA TGCAGCCGAC CAATCCGCAG TCGGCCCTGG TGCCAATGGG TCCAGTTGCG 
TCGGCTTCGA TTCCTCCTCA ATACGGCTTT CCACCTCATC AGCAACGTCC AACGGCTGCT 
CAGCCCACAC CGTACGGCAT GGTTGCAGGT GGAGTTGTTG GTGGACCGGC TTACGGTGAC 
CTGCAGTTGG TGCCTTACCA ACCAGCTGCT CAGCAACAGA GGCCACTAAA CAGCGAGGAG 
CTGCAGCGGC TGTACAGCAT GCCCGCTCAA ATGGCCGTGG TTCCAGTGCC GCAGCCAAAC 
GCCTATATGT ACTATCCCGG AGCTGTGGTT ACTCCATACA CGGCTCCCAT TGTTCCCGGA 
TCGGCTGCTT TTATGCCGCC GCAGTATCCC GCACAGGGAT ATGGCTTTGG AGGTGCTTAC 
ACGCACATGG ATTTGCGTCG ACCCCAATCG CAACCAGCTC CCCAACAAAC AGCACCGACA 
ACAAGTCATC ATCACAGCCA ACCGTCCAAC CATTCCACTT CCTCCCCCGC AGAGGCCAAT 
GGAGTAGCCT TCCCAGCGCG TCGCCAAGTG CCCTCGACTG TCGGGGTTAG CTCTAGTAGC 
CACACTGGAA ACAATGGTCA TTCCTCGGTC CCACGCAGGG GCAACGATTT GATCGACCTC 
AACCACGAGG ACTACTCCCG TGTGAGTGTG CTGGAGGCAT TCGATCCCCT GCTAAACGAC 
AATACTGGCA ACGACACCGC CTCCGACAGC ACTTCCTACT ATGCGGAATA CGATCCCTTT 
GATTTTCTGT ACAGCGGAGA TGCAGCAACC CAATATTCCG ATCCAATGTA TGAGGCAGTC 
AACAGGTGGG ACAAAACTGT GGCCACCGTG AGTCCGAATG TTGGTCTAAT CGGTTGGCGC 
CAAGATTTTC TGAGCCAGCC ATCTACATCT TCATCGCAAT ATGGTGTTGC GCCGCCAGAG 
GAGAGTCTGA AGCTTGCGGA GAACGGATCT GGAACTATCT CGCCTCCTCC GCCGTTGCCG 
CCCCGGAACC AGCAGTGCTA TGAATCAAAC CAGGCAGCCA TGCCGGTCTC CAGGCCTCCT 
CAGTCTTCTG TTTTGACGGA CAGCTACACC TCCAGCATTC CGGCCAACGT GGTGCTGGAC 
CGGCGGAAAA CTTGTACACG ACTGTACGAA TTGATCAGCG ACCAGCGCAC TGATGATCCC 
GAACTTTTGG AATTTTACCA CATGGTAAAG GAGGTGAGGG CACGCTATCC GCATGACGAT 
GCGCCCACCA ATGTGGGACA TGTTGTGGCC GCCGAGTTTA ATTATCACTA CATGATGGAC 
ACCAGCATCA AAGTGATTGT GCATCCGGCT CTAAATACAC TTCAATCAAC GGTCCTGGCT 
GCGTCCATGG GCAAGGAACA GGTGAAGGGA TATGGAATGC CAGTAACATT CACTTGCGAT 
ATTGATTCGG TTGTGGCACA GGTGGTGGCA CAAGCTTTGG CCTCGCTGGA GGGACAAGTC 
AAGGGTACCG TCACAGATTA TGCGGTCAAG CCCATTGGTC TTCTGGAGTG GCTGGCACCC 
ACCTCGAGAC TGAGTCAGCT GGAGTGCGTG CACAATAGCT TCCAATTGGA GAAGGATGTA 
CATTTGGGCC TGTGTCTTAG TACGGCGGCA AACATGCAGG CTATTGCACG AACTGAGCGG 
GATGATGAGC ACGATGCGGA TTTGCTGCCG GAACATCTTC TTCCCAACGA GGTGGTGCCA 
ATTGTGACCT ACGACAATAT GATGATACTC ATCGAAACGC TGGAGATGGA GATTGACAAG 
CTGGAATCGG CGGCCGACGG AGTACCCGGA CGGAGTGTCG TGAGCTGCTC CGGAGTTGTC 
CAAGCAGTGA AGGCCATATG CGCACTGCTC GGTTCAATCG ACACAATGGA AATTGCACGA 
TGTGTTGCCG ATCTGAAGCG CATTTGCGAG GTGGAGCAAA AGAAGTACTC GACGGGCGCT 
AGCAACCCAG AGATTGTGAG TGACTATGGT GATTACGCTC AAGTTGTACT CCGCCCGCGC 
TCCATGCTGG AGCAGATCAA GGTCAAGTGC AACGAGCTGC GAGATGCAGT GCAAGAGCTG 
GTTGAATTGT ATGCGAATGT TTTCCGGGTG GCATTCTCCG TGAAGACGCC CGATTACTCA 
ACAACACCCA TACCCATTTC CTGCGTGTCC AAACCAATTG TGGTATGCAT TAGCTGCCTA 
CACAGGCCGC TGCCGAATTG GAAGTTCGAC GATTATTCCC TGTGCGTACA AATCGTTTAT 
GGAACGCGCC TGCTGTCGAA GCCGAATGTG CTGACCTGCT CCAACGATAC AAGTGGAGGC 
CTGTTTCCTC GTCTTAACTT CAGTGCCTGG CTGACTTTCG ATCAGCATCC CATCTGCACT 
CTGCCCAGGG AGGCGCGCCT TACGTTCGTG TTGTATGGAA AACAGGCGGC CAGCGAAGGC 
GAACCCAACG CCGATCAGAA TGGAGAGAGG CGTCAGGTGA CCACTGAACT GGGTTGGTGT 
TCGATCCAAC TGTTTGACTT TAAGCGAGTG ATGATCTGCG GCCCCTACTT ACTGTCTTTA 
TGGCCACCAA TGACGGACAA AATGCTTGGA CCAGCTCCGG CTCGAGGCTG TCATCCGCAA 
CCCGACTTTT GCCCCGTTTT GAGCATTGAA GTACCTCCGT ATGGAGGACG CATTGAGTTT 
CCTGAGCACC AGGAGGTGCC AAAACCTGCA CCACACTACG ATTTTGCCTC TCTGGATGCC 
AATCTTCAAG AGGAGCTGCT GGACACCGCA GAGCTGGGCT ACACAGGAGC CACAGAACGA 
CGTGAAGTGT TCTGGGAAAA ACGGCTCTAC CTGCAGAGCT ATCCCAATGC CCTGCCAAAG 
GTTCTTCATG CCGCTCACAG TTGGGATTAT GCCAATTTGA TCGATTTGCA TGCGCTGCTG 
CACTCCTGGG CACCACTCTC GCCATTGCAG TCGTTGGAGT TACTTCTGCC ACGATATCCG 
GATGCTAAGG TTCGCGAGAA AGCCGTGGAG TGGATCTCCA AGATGCCCAA CGACCAGCTC 
GTCGACTTTC TGCCTCAATT GGTGCAAAGT TTAAAACATG ACACATACGA AGGCTCGGCA 
ATGGCTCGAT TCTTGCTGTC CAAATGCCTG GAGTCACCGC GCTTTGCCCA TCACATGTAT 
TGGCTGCTTG TACACAGTCT GCCTGACGAT CCCCACAACT CTATTGGAGC AGCGATGGTG 
GATCAGGAGT ATGACGAGTC TCAGGTTACC CAGGTCCGTT ACTACCGCCG GAACAAAATG 
ATGCTGCGTG CTTTAATGGC GATTTGCGGC GAAAAGATGC TTCAGCGATT TATGTACCAG 
CACCGAATGT GTCAGAAACT TACTACTATT GCGGAGTCGG TTAAAGAGGC TAAGGAGTCG 
ATGCGTCAAA AAAGCCTAGC CGCAGGCATG GACGAGGTGC ACCAAGACTT ACTGGAGCAA 
CCCACTTGCC TACCGCTGGG ACCAGAACTG GAGGTAACTG GAGTGAGTGT GCGTAACTGT 
AGCTACTTTA ACTCCAACAC GCTGCCGCTG AAGATCAACT TTGTGGGACC TGATGCCGAA 
TCTTTACCGG CTATCTTTAA GTGCGGAGAT GACTTGCAGC AGGATCAGTT AACTATACAG 
CTAATTAGGA TTATGAACAA AATGTGGTTG GCCGAACGAT TGGACCTGAA GATGGTCACC 
TTTAATTGTG TGCCTACGGG ATACAAGAGC GGTATGATTG AGCTGGTTAG CGAGGCGGAA 
ACGTTGAGAA AAATTCAAGT AGAGTGCGGT CTGACGGGGT CCTTTAAGGA TCGCCCGATC 
GCTGAGTGGT TAGGCAAGCA GAATCCCAGT CCTCTCGAGT ACCAGAGTGC TGTGCGAAAT 
TTTACGCTAT CCTGTGCTGG ATACAGTGTG GCCACGTATG TGCTAGGCAT CTGTGATCGC 
CACAATGACA ACATCATGTT AAAGACTTCG GGTCACTTGT TTCACATTGA CTTTGGCAAG 
TTTCTTGGCG ATGCTCAGAT GTTTGGAAAC TTTAAGAGAG ATCGCACTCC ATTTGTCCTG 
ACTTCCGACA TGGCTTATGT CATAAATGGC GGCGATAAGC CCTCCACAGA CTTTCACTAT 
TTCGTGGACC TATGTTGTCG AGCCTTTAAT ATCGTGCGGA AAAATGCTGA TCTACTCTTG 
CACACCCTGG CCCACATGGC TACAGCAGGC ATGCCGGGAG TAAACTCCAA TGCTGTGCAA 
TATGTACGAC GCGCCCTATT GCCATCTCAA TCGAATCCCG AGGCAGCTGC CACATTTGCC 
AAGATGATTC AATCCTCTTT GAAAAGCTGG TTCACGCAAT TCAATTTCTT TCTGCACAAT 
CTGGCCCAGA TGCGTTTCAC CCCAGACGAG GGATCAGGAG AGCTGCTATC GTTCGTGCCA 
CGAAAATATA CAATGCAGCA GGATGGTCGC TTGAAGATTG TAAAGGTGGT GTGTTTCCAG 
AAGCATTACA GCATGGAAAA GTTTTATATG TATATTCTGG AAGTGACGCG ACATGGACAG 
CCCGATCCGA CACATTTGTT CCGGTCATAT CGGGAATTCA CGGAATTCCA TCAGAAGTTA 
TGCATGCACT TTCCTTTGGT TAAACTGCAC AGTCTGCCGG CTGGTGTGCA TGTGGGCCGT 
TCCAATATCA AATCCGTGGC AGAAAAACGA CTACCTCTTA TTCAGCGATT TTTGAAATCG 
TTGTTCGATG CGTCCGAGGA AATAGCCCAT TCCGAGCTCG TTTACACATT CTTTCACCCG 
CTGCTGCGCG ATCAGCAGGA AGCCAAGCTT GGGATGCCGA AGATAAAGGA GGTGAAGCAA 
CAACCGTCGC GGGATAATCC CCACGAGATT GGCCAAATAC GACTATCGCT GCAATATCAA 
CGCGGCGTAC TTACTGTGAT GATACACCAC GCCAAAGGAC TGCCCATGTT ACAGGGCGGT 
CAGGAGCCCA ACACATATGT GAAGTGCTAC CTAAAACCGG ATCCCAAAAA GGAGACCAAA 
CGCAAGACCA AAGTGGTGCG CAAGACCTGT GTGCCCAGTT TCATGGAAAC TTTGGAGTAC 
CGAATGCCAC TGAATATTAT TCAAGAGCGC CGCCTTCAGG TTACGGTTTG GTCGCACGAC 
ACCCTGCAGG AGAACGAGCT GCTTGGAGGC TTCGATATGG ATCTGTCGAA GTACGACCTG 
CGACAGGAGC TCGTCGACTG GTATCGCCTG GGCGCGGTGT CCAGGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pi3K68D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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