Doc1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Doc1
Entrez Gene ID	39039
Full Name	Dorsocross1
Synonyms	CG5133,Dm-DOC1,Dmel\CG5133,Doc,Ect2,TBX6,Tb66F2,doc,doc1
General protein information	Preferred Names Dorsocross1 Names CG5133 gene product from transcript CG5133-RA CG5133-PA CG5133-PB Doc1-PA Doc1-PB dorsocross
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66F2-66F3

mRNA and Protein(s)

mRNA	Protein	Name
NM_140026.3	NP_648283.1	Dorsocross1, isoform A
NM_001274683.1	NP_001261612.1	Dorsocross1, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Doc2	NP_648282.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005896	XP_315925.4
Drosophila melanogaster (fruit fly)	Doc3	NP_648280.1
Drosophila melanogaster (fruit fly)	Doc1	NP_648283.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dorsocross T-box transcription factors promote tissue morphogenesis in the Drosophila wing imaginal disc
Title: The Dorsocross T-box transcription factors promote tissue morphogenesis in the Drosophila wing imaginal disc.

Genetic interaction analysis shows that spir functionally interacts with Dorsocross, tin, and pannier to properly specify the cardiac fate.
Title: Spire, an actin nucleation factor, regulates cell division during Drosophila heart development.

Required for the formation of all myocardial and pericardial cell types, with the exception of the Eve-positive pericardial cells.
Title: The Dorsocross T-box genes are key components of the regulatory network controlling early cardiogenesis in Drosophila.

Dorsocross genes are required for normal patterning of the dorsolateral ectoderm and, in particular, the repression of wingless and the ladybird homeobox genes within this area of the germ band
Title: The T-box-encoding Dorsocross genes function in amnioserosa development and the patterning of the dorsolateral germ band downstream of Dpp.

The following Doc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Doc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17980	NM_140026.3 Latest version!	Drosophila melanogaster Dorsocross1 (Doc1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa20771	NM_001274683.1 Latest version!	Drosophila melanogaster Dorsocross1 (Doc1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Doc1 ( NM_140026.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648283.1 Drosophila melanogaster Dorsocross1 (Doc1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17980
Clone ID Related Accession (Same CDS sequence)	NM_140026.3
Accession Version	NM_140026.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Dorsocross1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140026.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTTTCCA TGCAAGAGCT AATGGATATG CGGATGCAGC AGCAGCTGGC CCACGAGATC 
TACCGCCAGC AGATCATGCA GCGGATTCCA GATCCCTTTC CATCGATGTT ACCTTTCAAC 
ATGCCCCACC AGCAGCCGCA CATGATGCTG CCCAGTCGTC CCACCCTGCC CGGCGTGGAG 
GCCAAGTTGG AGAACAACGA TTTGTGGCAA CAGTTCCATA AAATCGGCAC CGAGATGATC 
ATCACAAAGA GCGGCCGACG CATGTTCCCC TCGATGCGAG TTTCCCTCAG CGGACTGGAG 
GAGGAGGCCA GCTATTGCGT ACTCCTCGAG ATGGTGCCCA TTGGCGATTG CCGCTACAAG 
TTCTCCGGTT CCCAGTGGGT TCCGGCCGGA GGAGCCGAGC CCCAGAGTCC GCAGCGCATG 
TATCTGCATC CGGACAGTCC GGCGACCGGT GCCCATTGGC AATCCCAGGC ATTGCTCTTC 
AACAAGGTCA AACTGACCAA CAATACACTG GACAGCAGCG GACATATCGT TTTGGCCAGC 
ATGCATAAGT ACCAGCCACG CCTGCACATC ATCCGCAGCA GCGAGCTCAC CCAACTTCCA 
TGGGCTCCTC AGCAGGCGTT CGTTTTCCCG GAGACGGAGT TCGTAGCTGT CACAGCCTAT 
CAGAACGATC GCATCACCAA ATTGAAGATC GATAACAATC CGTTCGCTAA GGGTTTCCGC 
GAGTCGGGTC AATCCCGCTG CAAAAGGAAG CTGAACAGCA GCGGAAACTC GACGCTGGAG 
TCGGAGGATG ATGGCTCCAG TGTGAGCAGC TGCGATTCTC CGCAGGCGAA GCGCCAGCGC 
CAGGATTTTG AGCAGGACTC AACGGGTAGT GTTTCGCCAG CTTACTATGG AGCAACTCAT 
CCAGTTGCCA ATCCTATGTC GCCGTATCTC CGCCATCACT TGAGCTATGG ACCCAGTGGA 
GCTGCCGGAT TGCCGCCCAG CATTGCAGCT GCCTATTTTG GTGGAGTTCC TCCGCAGATT 
CTGCCAATGC CACCAGCCAC ATATGCGCCA ACTGTCGCGC CTGTTAGTCC TGTGGCCTCG 
CAACCCGTTG GCTCCAGCAC ACCCACAACA TCACCAAGGC CTTCAACCTC AACGAAAAGA 
AATAGCTTCA GCATTTCAGC CATTTTGGCC TATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648283.1
CDS	166..1341
Translation	MLSMQELMDMRMQQQLAHEIYRQQIMQRIPDPFPSMLPFNMPHQQPHMMLPSRPTLPGVEAKLENNDLWQQFHKIGTEMIITKSGRRMFPSMRVSLSGLEEEASYCVLLEMVPIGDCRYKFSGSQWVPAGGAEPQSPQRMYLHPDSPATGAHWQSQALLFNKVKLTNNTLDSSGHIVLASMHKYQPRLHIIRSSELTQLPWAPQQAFVFPETEFVAVTAYQNDRITKLKIDNNPFAKGFRESGQSRCKRKLNSSGNSTLESEDDGSSVSSCDSPQAKRQRQDFEQDSTGSVSPAYYGATHPVANPMSPYLRHHLSYGPSGAAGLPPSIAAAYFGGVPPQILPMPPATYAPTVAPVSPVASQPVGSSTPTTSPRPSTSTKRNSFSISAILAY

Target ORF information:

RefSeq Version	NM_140026.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Dorsocross1 (Doc1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140026.3

ORF Insert Sequence:

ATGCTTTCCA TGCAAGAGCT AATGGATATG CGGATGCAGC AGCAGCTGGC CCACGAGATC 
TACCGCCAGC AGATCATGCA GCGGATTCCA GATCCCTTTC CATCGATGTT ACCTTTCAAC 
ATGCCCCACC AGCAGCCGCA CATGATGCTG CCCAGTCGTC CCACCCTGCC CGGCGTGGAG 
GCCAAGTTGG AGAACAACGA TTTGTGGCAA CAGTTCCATA AAATCGGCAC CGAGATGATC 
ATCACAAAGA GCGGCCGACG CATGTTCCCC TCGATGCGAG TTTCCCTCAG CGGACTGGAG 
GAGGAGGCCA GCTATTGCGT ACTCCTCGAG ATGGTGCCCA TTGGCGATTG CCGCTACAAG 
TTCTCCGGTT CCCAGTGGGT TCCGGCCGGA GGAGCCGAGC CCCAGAGTCC GCAGCGCATG 
TATCTGCATC CGGACAGTCC GGCGACCGGT GCCCATTGGC AATCCCAGGC ATTGCTCTTC 
AACAAGGTCA AACTGACCAA CAATACACTG GACAGCAGCG GACATATCGT TTTGGCCAGC 
ATGCATAAGT ACCAGCCACG CCTGCACATC ATCCGCAGCA GCGAGCTCAC CCAACTTCCA 
TGGGCTCCTC AGCAGGCGTT CGTTTTCCCG GAGACGGAGT TCGTAGCTGT CACAGCCTAT 
CAGAACGATC GCATCACCAA ATTGAAGATC GATAACAATC CGTTCGCTAA GGGTTTCCGC 
GAGTCGGGTC AATCCCGCTG CAAAAGGAAG CTGAACAGCA GCGGAAACTC GACGCTGGAG 
TCGGAGGATG ATGGCTCCAG TGTGAGCAGC TGCGATTCTC CGCAGGCGAA GCGCCAGCGC 
CAGGATTTTG AGCAGGACTC AACGGGTAGT GTTTCGCCAG CTTACTATGG AGCAACTCAT 
CCAGTTGCCA ATCCTATGTC GCCGTATCTC CGCCATCACT TGAGCTATGG ACCCAGTGGA 
GCTGCCGGAT TGCCGCCCAG CATTGCAGCT GCCTATTTTG GTGGAGTTCC TCCGCAGATT 
CTGCCAATGC CACCAGCCAC ATATGCGCCA ACTGTCGCGC CTGTTAGTCC TGTGGCCTCG 
CAACCCGTTG GCTCCAGCAC ACCCACAACA TCACCAAGGC CTTCAACCTC AACGAAAAGA 
AATAGCTTCA GCATTTCAGC CATTTTGGCC TATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Doc1 ( NM_001274683.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261612.1 Drosophila melanogaster Dorsocross1 (Doc1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa20771
Clone ID Related Accession (Same CDS sequence)	NM_001274683.1
Accession Version	NM_001274683.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Dorsocross1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTACCTT TCAACATGCC CCACCAGCAG CCGCACATGA TGCTGCCCAG TCGTCCCACC 
CTGCCCGGCG TGGAGGCCAA GTTGGAGAAC AACGATTTGT GGCAACAGTT CCATAAAATC 
GGCACCGAGA TGATCATCAC AAAGAGCGGC CGACGCATGT TCCCCTCGAT GCGAGTTTCC 
CTCAGCGGAC TGGAGGAGGA GGCCAGCTAT TGCGTACTCC TCGAGATGGT GCCCATTGGC 
GATTGCCGCT ACAAGTTCTC CGGTTCCCAG TGGGTTCCGG CCGGAGGAGC CGAGCCCCAG 
AGTCCGCAGC GCATGTATCT GCATCCGGAC AGTCCGGCGA CCGGTGCCCA TTGGCAATCC 
CAGGCATTGC TCTTCAACAA GGTCAAACTG ACCAACAATA CACTGGACAG CAGCGGACAT 
ATCGTTTTGG CCAGCATGCA TAAGTACCAG CCACGCCTGC ACATCATCCG CAGCAGCGAG 
CTCACCCAAC TTCCATGGGC TCCTCAGCAG GCGTTCGTTT TCCCGGAGAC GGAGTTCGTA 
GCTGTCACAG CCTATCAGAA CGATCGCATC ACCAAATTGA AGATCGATAA CAATCCGTTC 
GCTAAGGGTT TCCGCGAGTC GGGTCAATCC CGCTGCAAAA GGAAGCTGAA CAGCAGCGGA 
AACTCGACGC TGGAGTCGGA GGATGATGGC TCCAGTGTGA GCAGCTGCGA TTCTCCGCAG 
GCGAAGCGCC AGCGCCAGGA TTTTGAGCAG GACTCAACGG GTAGTGTTTC GCCAGCTTAC 
TATGGAGCAA CTCATCCAGT TGCCAATCCT ATGTCGCCGT ATCTCCGCCA TCACTTGAGC 
TATGGACCCA GTGGAGCTGC CGGATTGCCG CCCAGCATTG CAGCTGCCTA TTTTGGTGGA 
GTTCCTCCGC AGATTCTGCC AATGCCACCA GCCACATATG CGCCAACTGT CGCGCCTGTT 
AGTCCTGTGG CCTCGCAACC CGTTGGCTCC AGCACACCCA CAACATCACC AAGGCCTTCA 
ACCTCAACGA AAAGAAATAG CTTCAGCATT TCAGCCATTT TGGCCTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261612.1
CDS	84..1154
Translation	MLPFNMPHQQPHMMLPSRPTLPGVEAKLENNDLWQQFHKIGTEMIITKSGRRMFPSMRVSLSGLEEEASYCVLLEMVPIGDCRYKFSGSQWVPAGGAEPQSPQRMYLHPDSPATGAHWQSQALLFNKVKLTNNTLDSSGHIVLASMHKYQPRLHIIRSSELTQLPWAPQQAFVFPETEFVAVTAYQNDRITKLKIDNNPFAKGFRESGQSRCKRKLNSSGNSTLESEDDGSSVSSCDSPQAKRQRQDFEQDSTGSVSPAYYGATHPVANPMSPYLRHHLSYGPSGAAGLPPSIAAAYFGGVPPQILPMPPATYAPTVAPVSPVASQPVGSSTPTTSPRPSTSTKRNSFSISAILAY

Target ORF information:

RefSeq Version	NM_001274683.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Dorsocross1 (Doc1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274683.1

ORF Insert Sequence:

ATGTTACCTT TCAACATGCC CCACCAGCAG CCGCACATGA TGCTGCCCAG TCGTCCCACC 
CTGCCCGGCG TGGAGGCCAA GTTGGAGAAC AACGATTTGT GGCAACAGTT CCATAAAATC 
GGCACCGAGA TGATCATCAC AAAGAGCGGC CGACGCATGT TCCCCTCGAT GCGAGTTTCC 
CTCAGCGGAC TGGAGGAGGA GGCCAGCTAT TGCGTACTCC TCGAGATGGT GCCCATTGGC 
GATTGCCGCT ACAAGTTCTC CGGTTCCCAG TGGGTTCCGG CCGGAGGAGC CGAGCCCCAG 
AGTCCGCAGC GCATGTATCT GCATCCGGAC AGTCCGGCGA CCGGTGCCCA TTGGCAATCC 
CAGGCATTGC TCTTCAACAA GGTCAAACTG ACCAACAATA CACTGGACAG CAGCGGACAT 
ATCGTTTTGG CCAGCATGCA TAAGTACCAG CCACGCCTGC ACATCATCCG CAGCAGCGAG 
CTCACCCAAC TTCCATGGGC TCCTCAGCAG GCGTTCGTTT TCCCGGAGAC GGAGTTCGTA 
GCTGTCACAG CCTATCAGAA CGATCGCATC ACCAAATTGA AGATCGATAA CAATCCGTTC 
GCTAAGGGTT TCCGCGAGTC GGGTCAATCC CGCTGCAAAA GGAAGCTGAA CAGCAGCGGA 
AACTCGACGC TGGAGTCGGA GGATGATGGC TCCAGTGTGA GCAGCTGCGA TTCTCCGCAG 
GCGAAGCGCC AGCGCCAGGA TTTTGAGCAG GACTCAACGG GTAGTGTTTC GCCAGCTTAC 
TATGGAGCAA CTCATCCAGT TGCCAATCCT ATGTCGCCGT ATCTCCGCCA TCACTTGAGC 
TATGGACCCA GTGGAGCTGC CGGATTGCCG CCCAGCATTG CAGCTGCCTA TTTTGGTGGA 
GTTCCTCCGC AGATTCTGCC AATGCCACCA GCCACATATG CGCCAACTGT CGCGCCTGTT 
AGTCCTGTGG CCTCGCAACC CGTTGGCTCC AGCACACCCA CAACATCACC AAGGCCTTCA 
ACCTCAACGA AAAGAAATAG CTTCAGCATT TCAGCCATTT TGGCCTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Doc1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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