Dg cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dg
Entrez Gene ID	36773
Full Name	Dystroglycan
Synonyms	CG18250,CT41273,DG,DmDG,Dmel\CG18250,atu,dg,dgn,dys
General protein information	Preferred Names Dystroglycan Names CG18250 gene product from transcript CG18250-RD CG18250-PA CG18250-PB CG18250-PC CG18250-PD Dg-PA Dg-PB Dg-PC Dg-PD alphaDystroglycan atsugari dystroglican dystroglycan dystroglycan-like
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 52E2-52E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_079032.4	NP_523756.1	dystroglycan, isoform A
NM_166138.5	NP_725523.3	dystroglycan, isoform B
NM_176184.4	NP_788364.2	dystroglycan, isoform C
NM_176183.3	NP_788363.1	dystroglycan, isoform D

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005162	XP_001688514.1
Drosophila melanogaster (fruit fly)	Dg	NP_725523.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

in Drosophila the microRNA complex miR-310s acts as an executive mechanism to buffer levels of the muscular dystrophy-associated extracellular matrix receptor dystroglycan via its alternative 3'-UTR
Title: miRNA-based buffering of the cobblestone-lissencephaly-associated extracellular matrix receptor dystroglycan via its alternative 3'-UTR.

Nrk, mbl, capt and Cam genetically interact with dystrophin and/or dystroglycan in the process of axon path-finding in the eye.
Title: New dystrophin/dystroglycan interactors control neuron behavior in Drosophila eye.

only dystroglycan, but not dystrophin deficiency causes myodegeneration induced by energetic stress suggesting that dystroglycan might be a component of the low-energy pathway and act as a transducer of energetic stress in normal and dystrophic muscles
Title: Stress and muscular dystrophy: a genetic screen for dystroglycan and dystrophin interactors in Drosophila identifies cellular stress response components.

photoreceptor(R) cell differentiation defects appear at the same stage in a deficiency line Df(2R)Dg(248) that affects Dystroglycan (DG) and the neighboring mitochondrial ribosomal gene, mRpL34; findings discussed in view of recent work implicating DG as a regulator of cell metabolism and its genetic interaction with mRpL34
Title: Dystroglycan and mitochondrial ribosomal protein L34 regulate differentiation in the Drosophila eye.

reduced expression of dystroglycan induced tolerance to cold as well as preference for low temperature; sustained increase in mitochondrial oxidative metabolism caused by reduced expression of DmDG accounted for the cryophilic phenotype of the atu mutant
Title: Changes in temperature preferences and energy homeostasis in dystroglycan mutants.

The following Dg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10159	NM_166138.5 Latest version!	Drosophila melanogaster dystroglycan, transcript variant B (Dg), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa10158	NM_176183.3 Latest version!	Drosophila melanogaster dystroglycan, transcript variant D (Dg), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa10160	NM_079032.4 Latest version!	Drosophila melanogaster dystroglycan, transcript variant A (Dg), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa10161	NM_176184.4 Latest version!	Drosophila melanogaster dystroglycan, transcript variant C (Dg), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dg ( NM_166138.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725523.3 Drosophila melanogaster dystroglycan, transcript variant B (Dg), mRNA.

Price & Availability↑

CloneID	OFa10159
Clone ID Related Accession (Same CDS sequence)	NM_166138.5
Accession Version	NM_166138.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dystroglycan, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166138.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGATTCC AGTGGTTCTT ATCGGCCAGT GCGAGCTTGT CGCTGTTCCT TCTCCTGGAC 
TTCGTCGTAT GGATTCACGG CGAGCGGGAC TTCAATGTCA ACGACTCCCA GGTCCCTGTA 
ATTGAGCCAA AGGATGTGCC CGCCTACAAA CAGGATCCGT ATGTTACGGA ATTGATGAGT 
TGCCAAAATA CTCCGAGTGA AATTGTGCTT TCACTTTTGT TGAAGAAGCA CGACTGGAGT 
GAGCTGACGG CCACGAAACG AGCTCATGTC CAGGCCAAGT TGGCCAAGTT CTTTGCCATA 
CCCAAGGAAT TCATTTCCCT GGATTCGGTG TCTAAACGCG AACTGAGATC CATGCACAAG 
TTGGCCATGA GAAAAGGAGG CAAGGGCAAC AAGAATATCG AGACCCTGAA TCGTCGCCTG 
GGACGCGCCA GTTTCATGAT CGGCTGTGGT CCAAGCTACT TTGTGATGGG CGAACCGATA 
GCCAAGCAGA TTGCCCACCA AATGAAGGAT GGCACCATTG GCGCTTTGAC GGAGGAGAAC 
TTCGGGCTGT GGTTCATTTG GCGAAAGGAA TTGAAATCAA GATCAAACCG CAAGCGACGT 
CAATCCGAGG GCTCTGGTGC CGATGAAGAT GACTACGACT ATGGGGATGA CGATGAGGAA 
GTTGCTGAGC CCAGTACGGA AGTGCCACCA GTGACCACAC ATGCCCATCG ACATCATCAC 
GGAGCGGAAG GAGGAGAACC CGATGCCATC AGCAGCGGCA ACAATAGCCT GGCCAATAAT 
GAGCTGTCGA CGCCCGCCAC GCCCACATCT TCGGTGGCCA CGACCACGGC ATCTACTCCG 
GAGTCGAGCA GCATCAGCAG CAACTTTGTC TCCACCGACT ACATGGAACC GCAGCCGGAA 
GAGAATAGTC CGCCCATCAT CAAGACACGC CTGCAGAAGC TGGCTGTCAC ATCGGGCAAG 
GCCTTCACCT TCCATGTGCT ACCGGAAACG TTTTACGATG CCGAGGATCA GGGTAATCTC 
CGCCTGGCGC TCACCGACAA GGATGGCCAC GAGCTAAAGG CGAACTCTTG GCTGCAGTTT 
AACGCCGACA AGAGGGAGCT TTATGGCCTA CCTCTGGACG ACACGGTATC CCGCTGGCAA 
TATCGCCTGT CGGCCACGGA TTCGGGCAAC GCCAGTGTCA CGGAAACCGT GGAGATAAGT 
GTGCAGCAGC ATCGAGCGGT TAGGACCATT AACCACGAGA TCAGTGTTTT TGTACGGATC 
AACGAGAAGC CGGGTCACAA CATTGATTGG CAGTTGAAGC TCATTAATGC GGTGGCCAGA 
ACCTTGGATG ACTCCACCAA TTCGGCAGTG GTGGTGCGTG AGATCCGACA GACACCACAC 
GATCCACACA GTGCCACCTT CGTGTATTTT AATGAAACGC TGCCCACCAG CGAGTGTCCC 
GAAAAGGAAC TAAAGGATAT TATTGCACGA CTGGATGCCA ATAGACTGAG TGATTTGGTT 
CAGCCACAGC TGGGAATCAA ATCTATAACA GGACAGTTAA TCGGATCCTG TCAGAAGGAT 
TTAACTCAGG TGAAGCCCAC GCAACACATG ACCAAGAATG TGCCTCCAAT GCCACGCAAC 
CAGGTGGATC GCGTTAACGC TAGCTTGGGC CAGCTGCTGG TCTACAAAGT GCCGGCGGAT 
ACCTTCTATG ATGCGAACGA CAATCAATTG ACTCTTACGT TGAAAACCAG GGATCACCTT 
GAGCTTAGCC CACGCCACTG GCTGCAGTTC GACTCCAAGA ACGAAGAGTT CTATGGCATT 
CCGAAAAGCG GTGACATTGG TTCCGAGGAA TATCTGTTGG TCGCCGAAGA CAGTGGTGGC 
CTGAGTGCCC ATGATGCCCT GGTTGTAGTG GTTAGCCCAG CGCCAAAGCG TGACTTTGGG 
TTCTTCTTCA AAGCGTATCT GTCTATCAAA CACGAGCGAT TCAATGCCGA TCTGCAGCGG 
AAGTTTGTAG AGCGTGTAGC GAAGCTGAAT GGAGATCCTA CTACTGGCCA GATCCAGATC 
CGTTCGATAA CCACGCACCA CGACTCCGAT GGCACCATAG TGAACTTCTA CAATACGACG 
CTGTACAGGA AGCACAACAG TTGTCGGGAG AAGGAAGTGG CCATGACTAG GAGTGTCTAC 
CTAAACAGTG ACCTCAGCCT GAGGGAGGCA GCCAAACGGG CTCTGGGACC CGAGCTCAAT 
TTGACCAACT TTTCGGTGGT GCCCTTCAGT ATTTGTCATC ATACTGAGAA CATAGACACC 
AACCAACTTG ATTATATACC TAGTCGCCCC GAAGAGCCTA CACATAAGTC CTCTTTCGGC 
GAGGATTACA TGATCACGTT CGTATGGCCC ATCGTGATCA TTGTGGCGAT GCTTGTGGCT 
GCCTCAATCA TTGCCTGTTG CCTGCACTGG TGTCGCCAAA GGAGCGGCAA AATGGAGCTA 
GGCGACGAAG AGGAGCGCAA GTCCTTCCGT GCCAAGGGTA TTCCCGTCAT CTTTCAGGAC 
GAGTACGAGG AGAAGCCCGA GATCGGCAAC AAGAGTCCTG TCATACTCAA GGACGAGAAG 
CCGCCGCTGC TGCCACCATC CTACAATACC TCAAACATGA ATGGTGACAA CGACGTGGAC 
GATTATGTGC CACCACCATC GGTGGTTGTG GGCGGCCGGG AAGTGCGTGG CAAGTCGCCA 
GCCACGCCCT CCTACCGCAA ACCACCGCCA TATGTGTCGC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725523.3
CDS	649..3393
Translation	MRFQWFLSASASLSLFLLLDFVVWIHGERDFNVNDSQVPVIEPKDVPAYKQDPYVTELMSCQNTPSEIVLSLLLKKHDWSELTATKRAHVQAKLAKFFAIPKEFISLDSVSKRELRSMHKLAMRKGGKGNKNIETLNRRLGRASFMIGCGPSYFVMGEPIAKQIAHQMKDGTIGALTEENFGLWFIWRKELKSRSNRKRRQSEGSGADEDDYDYGDDDEEVAEPSTEVPPVTTHAHRHHHGAEGGEPDAISSGNNSLANNELSTPATPTSSVATTTASTPESSSISSNFVSTDYMEPQPEENSPPIIKTRLQKLAVTSGKAFTFHVLPETFYDAEDQGNLRLALTDKDGHELKANSWLQFNADKRELYGLPLDDTVSRWQYRLSATDSGNASVTETVEISVQQHRAVRTINHEISVFVRINEKPGHNIDWQLKLINAVARTLDDSTNSAVVVREIRQTPHDPHSATFVYFNETLPTSECPEKELKDIIARLDANRLSDLVQPQLGIKSITGQLIGSCQKDLTQVKPTQHMTKNVPPMPRNQVDRVNASLGQLLVYKVPADTFYDANDNQLTLTLKTRDHLELSPRHWLQFDSKNEEFYGIPKSGDIGSEEYLLVAEDSGGLSAHDALVVVVSPAPKRDFGFFFKAYLSIKHERFNADLQRKFVERVAKLNGDPTTGQIQIRSITTHHDSDGTIVNFYNTTLYRKHNSCREKEVAMTRSVYLNSDLSLREAAKRALGPELNLTNFSVVPFSICHHTENIDTNQLDYIPSRPEEPTHKSSFGEDYMITFVWPIVIIVAMLVAASIIACCLHWCRQRSGKMELGDEEERKSFRAKGIPVIFQDEYEEKPEIGNKSPVILKDEKPPLLPPSYNTSNMNGDNDVDDYVPPPSVVVGGREVRGKSPATPSYRKPPPYVSP

Target ORF information:

RefSeq Version	NM_166138.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dystroglycan, transcript variant B (Dg), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166138.5

ORF Insert Sequence:

ATGAGATTCC AGTGGTTCTT ATCGGCCAGT GCGAGCTTGT CGCTGTTCCT TCTCCTGGAC 
TTCGTCGTAT GGATTCACGG CGAGCGGGAC TTCAATGTCA ACGACTCCCA GGTCCCTGTA 
ATTGAGCCAA AGGATGTGCC CGCCTACAAA CAGGATCCGT ATGTTACGGA ATTGATGAGT 
TGCCAAAATA CTCCGAGTGA AATTGTGCTT TCACTTTTGT TGAAGAAGCA CGACTGGAGT 
GAGCTGACGG CCACGAAACG AGCTCATGTC CAGGCCAAGT TGGCCAAGTT CTTTGCCATA 
CCCAAGGAAT TCATTTCCCT GGATTCGGTG TCTAAACGCG AACTGAGATC CATGCACAAG 
TTGGCCATGA GAAAAGGAGG CAAGGGCAAC AAGAATATCG AGACCCTGAA TCGTCGCCTG 
GGACGCGCCA GTTTCATGAT CGGCTGTGGT CCAAGCTACT TTGTGATGGG CGAACCGATA 
GCCAAGCAGA TTGCCCACCA AATGAAGGAT GGCACCATTG GCGCTTTGAC GGAGGAGAAC 
TTCGGGCTGT GGTTCATTTG GCGAAAGGAA TTGAAATCAA GATCAAACCG CAAGCGACGT 
CAATCCGAGG GCTCTGGTGC CGATGAAGAT GACTACGACT ATGGGGATGA CGATGAGGAA 
GTTGCTGAGC CCAGTACGGA AGTGCCACCA GTGACCACAC ATGCCCATCG ACATCATCAC 
GGAGCGGAAG GAGGAGAACC CGATGCCATC AGCAGCGGCA ACAATAGCCT GGCCAATAAT 
GAGCTGTCGA CGCCCGCCAC GCCCACATCT TCGGTGGCCA CGACCACGGC ATCTACTCCG 
GAGTCGAGCA GCATCAGCAG CAACTTTGTC TCCACCGACT ACATGGAACC GCAGCCGGAA 
GAGAATAGTC CGCCCATCAT CAAGACACGC CTGCAGAAGC TGGCTGTCAC ATCGGGCAAG 
GCCTTCACCT TCCATGTGCT ACCGGAAACG TTTTACGATG CCGAGGATCA GGGTAATCTC 
CGCCTGGCGC TCACCGACAA GGATGGCCAC GAGCTAAAGG CGAACTCTTG GCTGCAGTTT 
AACGCCGACA AGAGGGAGCT TTATGGCCTA CCTCTGGACG ACACGGTATC CCGCTGGCAA 
TATCGCCTGT CGGCCACGGA TTCGGGCAAC GCCAGTGTCA CGGAAACCGT GGAGATAAGT 
GTGCAGCAGC ATCGAGCGGT TAGGACCATT AACCACGAGA TCAGTGTTTT TGTACGGATC 
AACGAGAAGC CGGGTCACAA CATTGATTGG CAGTTGAAGC TCATTAATGC GGTGGCCAGA 
ACCTTGGATG ACTCCACCAA TTCGGCAGTG GTGGTGCGTG AGATCCGACA GACACCACAC 
GATCCACACA GTGCCACCTT CGTGTATTTT AATGAAACGC TGCCCACCAG CGAGTGTCCC 
GAAAAGGAAC TAAAGGATAT TATTGCACGA CTGGATGCCA ATAGACTGAG TGATTTGGTT 
CAGCCACAGC TGGGAATCAA ATCTATAACA GGACAGTTAA TCGGATCCTG TCAGAAGGAT 
TTAACTCAGG TGAAGCCCAC GCAACACATG ACCAAGAATG TGCCTCCAAT GCCACGCAAC 
CAGGTGGATC GCGTTAACGC TAGCTTGGGC CAGCTGCTGG TCTACAAAGT GCCGGCGGAT 
ACCTTCTATG ATGCGAACGA CAATCAATTG ACTCTTACGT TGAAAACCAG GGATCACCTT 
GAGCTTAGCC CACGCCACTG GCTGCAGTTC GACTCCAAGA ACGAAGAGTT CTATGGCATT 
CCGAAAAGCG GTGACATTGG TTCCGAGGAA TATCTGTTGG TCGCCGAAGA CAGTGGTGGC 
CTGAGTGCCC ATGATGCCCT GGTTGTAGTG GTTAGCCCAG CGCCAAAGCG TGACTTTGGG 
TTCTTCTTCA AAGCGTATCT GTCTATCAAA CACGAGCGAT TCAATGCCGA TCTGCAGCGG 
AAGTTTGTAG AGCGTGTAGC GAAGCTGAAT GGAGATCCTA CTACTGGCCA GATCCAGATC 
CGTTCGATAA CCACGCACCA CGACTCCGAT GGCACCATAG TGAACTTCTA CAATACGACG 
CTGTACAGGA AGCACAACAG TTGTCGGGAG AAGGAAGTGG CCATGACTAG GAGTGTCTAC 
CTAAACAGTG ACCTCAGCCT GAGGGAGGCA GCCAAACGGG CTCTGGGACC CGAGCTCAAT 
TTGACCAACT TTTCGGTGGT GCCCTTCAGT ATTTGTCATC ATACTGAGAA CATAGACACC 
AACCAACTTG ATTATATACC TAGTCGCCCC GAAGAGCCTA CACATAAGTC CTCTTTCGGC 
GAGGATTACA TGATCACGTT CGTATGGCCC ATCGTGATCA TTGTGGCGAT GCTTGTGGCT 
GCCTCAATCA TTGCCTGTTG CCTGCACTGG TGTCGCCAAA GGAGCGGCAA AATGGAGCTA 
GGCGACGAAG AGGAGCGCAA GTCCTTCCGT GCCAAGGGTA TTCCCGTCAT CTTTCAGGAC 
GAGTACGAGG AGAAGCCCGA GATCGGCAAC AAGAGTCCTG TCATACTCAA GGACGAGAAG 
CCGCCGCTGC TGCCACCATC CTACAATACC TCAAACATGA ATGGTGACAA CGACGTGGAC 
GATTATGTGC CACCACCATC GGTGGTTGTG GGCGGCCGGG AAGTGCGTGG CAAGTCGCCA 
GCCACGCCCT CCTACCGCAA ACCACCGCCA TATGTGTCGC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dg ( NM_176183.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788363.1 Drosophila melanogaster dystroglycan, transcript variant D (Dg), mRNA.

Price & Availability↑

CloneID	OFa10158
Clone ID Related Accession (Same CDS sequence)	NM_176183.3
Accession Version	NM_176183.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dystroglycan, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_176183.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGATTCC AGTGGTTCTT ATCGGCCAGT GCGAGCTTGT CGCTGTTCCT TCTCCTGGAC 
TTCGTCGTAT GGATTCACGG CGAGCGGGAC TTCAATGTCA ACGACTCCCA GGTCCCTGTA 
ATTGAGCCAA AGGATGTGCC CGCCTACAAA CAGGATCCGT ATGTTACGGA ATTGATGAGT 
TGCCAAAATA CTCCGAGTGA AATTGTGCTT TCACTTTTGT TGAAGAAGCA CGACTGGAGT 
GAGCTGACGG CCACGAAACG AGCTCATGTC CAGGCCAAGT TGGCCAAGTT CTTTGCCATA 
CCCAAGGAAT TCATTTCCCT GGATTCGGTG TCTAAACGCG AACTGAGATC CATGCACAAG 
TTGGCCATGA GAAAAGGAGG CAAGGGCAAC AAGAATATCG AGACCCTGAA TCGTCGCCTG 
GGACGCGCCA GTTTCATGAT CGGCTGTGGT CCAAGCTACT TTGTGATGGG CGAACCGATA 
GCCAAGCAGA TTGCCCACCA AATGAAGGAT GGCACCATTG GCGCTTTGAC GGAGGAGAAC 
TTCGGGCTGT GGTTCATTTG GCGAAAGGAA TTGAAATCAA GATCAAACCG CAAGCGACGT 
CAATCCGAGG GCTCTGGTGC CGATGAAGAT GACTACGACT ATGGGGATGA CGATGAGGAA 
GTTGCTGAGC CCAGTACGGA AGTGCCACCA GTGACCACAC ATGCCCATCG ACATCATCAC 
GGAGCGTTGG AGGTGTCGGA GAAGATAGTG TCGCCCGAAT CCGTCTCTTC GTCGGCTGTT 
CCCCTGGTTC CGGACGTGGA GGAGGAGATC GAAGAGAGCG TCTCCAAGCT GGAGTCCGTC 
ATCAGCAAGA CGATTGAGAA TACGAAGAAT ATTAAGGAGC TGCCCGTTCT GGGCGAAGCC 
GACGATGGTG AAGATGAAGA GGAAGTGGAA CTACAGCAAT TGGGAGTTCC ATTGGCCGCG 
GAGATTGTGC CAGACGAAAC CACCAGTGCG GCGGCCACAC GTGCCACGGA AATGGTAAAG 
CCCGTTGATT TGGATGAGCA AGAAATTTCG GTGGACTCCG GCGCTGTGCC GGCTATCGAT 
GTGGAGGCCT CGGCCACGCC CACGCCCTCG ATGTCCGCTC GCGAAACACA GAAGCCCTTT 
TCACCCTATG ACGCCACCTC TTCGGTGTCG TCCTCGTTCT CGCCCGTCGC ATCGATTGCA 
TCTGGCAGCG AAGCTGGTGA AGATTCAACT TCGGTGTCGA CCCCTCACCT GTCCCCAGCT 
AACTCACCCA CGGTCATGCC CACAGAGCTA GACAGAAATG GCCTAGCCAC CACATCATCC 
TCATCATCTT CATCCGCCTC ACCTCCTCCA TCGTCATCCG TTCCAACACA AGCCACTCAA 
CCAACCACAT CTGCATCATC ATTCTCATCA TCATCATCAA CAACAACAAC AAGCACAACA 
GCAACAGTCT CAACAACAAC AACAGCATCA TCAACAGCAA CAACAACTAC AACAACAACA 
ACACTGTCTG AATCGCCAAA GCCAAATACT GTGCTTAATG AGCTCGAGCT GAGCACCCAG 
CTGCCCATCG ACGATTTTGA AGGCACTGCT ACTGCAACAC AGCCAACTGG CACTGCAACT 
GCAACTGCCA TTGCAAGCAC AGCAGCAGCA GCAGCAGCAA CAGCAGCAGC AGCAACCGCA 
ACAGCCACAA CTGCCAATGC ATCTGCAACA ACAGCAGGCG AAAGCGAATT TCATATAGAG 
TCCACAACGC ACCGCACGAT TAGCTCTAAG GAAGGAGGAG AACCCGATGC CATCAGCAGC 
GGCAACAATA GCCTGGCCAA TAATGAGCTG TCGACGCCCG CCACGCCCAC ATCTTCGGTG 
GCCACGACCA CGGCATCTAC TCCGGAGTCG AGCAGCATCA GCAGCAACTT TGTCTCCACC 
GACTACATGG AACCGCAGCC GGAAGAGAAT AGTCCGCCCA TCATCAAGAC ACGCCTGCAG 
AAGCTGGCTG TCACATCGGG CAAGGCCTTC ACCTTCCATG TGCTACCGGA AACGTTTTAC 
GATGCCGAGG ATCAGGGTAA TCTCCGCCTG GCGCTCACCG ACAAGGATGG CCACGAGCTA 
AAGGCGAACT CTTGGCTGCA GTTTAACGCC GACAAGAGGG AGCTTTATGG CCTACCTCTG 
GACGACACGG TATCCCGCTG GCAATATCGC CTGTCGGCCA CGGATTCGGG CAACGCCAGT 
GTCACGGAAA CCGTGGAGAT AAGTGTGCAG CAGCATCGAG CGGTTAGGAC CATTAACCAC 
GAGATCAGTG TTTTTGTACG GATCAACGAG AAGCCGGGTC ACAACATTGA TTGGCAGTTG 
AAGCTCATTA ATGCGGTGGC CAGAACCTTG GATGACTCCA CCAATTCGGC AGTGGTGGTG 
CGTGAGATCC GACAGACACC ACACGATCCA CACAGTGCCA CCTTCGTGTA TTTTAATGAA 
ACGCTGCCCA CCAGCGAGTG TCCCGAAAAG GAACTAAAGG ATATTATTGC ACGACTGGAT 
GCCAATAGAC TGAGTGATTT GGTTCAGCCA CAGCTGGGAA TCAAATCTAT AACAGGACAG 
TTAATCGGAT CCTGTCAGAA GGATTTAACT CAGGTGAAGC CCACGCAACA CATGACCAAG 
AATGTGCCTC CAATGCCACG CAACCAGGTG GATCGCGTTA ACGCTAGCTT GGGCCAGCTG 
CTGGTCTACA AAGTGCCGGC GGATACCTTC TATGATGCGA ACGACAATCA ATTGACTCTT 
ACGTTGAAAA CCAGGGATCA CCTTGAGCTT AGCCCACGCC ACTGGCTGCA GTTCGACTCC 
AAGAACGAAG AGTTCTATGG CATTCCGAAA AGCGGTGACA TTGGTTCCGA GGAATATCTG 
TTGGTCGCCG AAGACAGTGG TGGCCTGAGT GCCCATGATG CCCTGGTTGT AGTGGTTAGC 
CCAGCGCCAA AGCGTGACTT TGGGTTCTTC TTCAAAGCGT ATCTGTCTAT CAAACACGAG 
CGATTCAATG CCGATCTGCA GCGGAAGTTT GTAGAGCGTG TAGCGAAGCT GAATGGAGAT 
CCTACTACTG GCCAGATCCA GATCCGTTCG ATAACCACGC ACCACGACTC CGATGGCACC 
ATAGTGAACT TCTACAATAC GACGCTGTAC AGGAAGCACA ACAGTTGTCG GGAGAAGGAA 
GTGGCCATGA CTAGGAGTGT CTACCTAAAC AGTGACCTCA GCCTGAGGGA GGCAGCCAAA 
CGGGCTCTGG GACCCGAGCT CAATTTGACC AACTTTTCGG TGGTGCCCTT CAGTATTTGT 
CATCATACTG AGAACATAGA CACCAACCAA CTTGATTATA TACCTAGTCG CCCCGAAGAG 
CCTACACATA AGTCCTCTTT CGGCGAGGAT TACATGATCA CGTTCGTATG GCCCATCGTG 
ATCATTGTGG CGATGCTTGT GGCTGCCTCA ATCATTGCCT GTTGCCTGCA CTGGTGTCGC 
CAAAGGAGCG GCAAAATGGA GCTAGGCGAC GAAGAGGAGC GCAAGTCCTT CCGTGCCAAG 
GGTATTCCCG TCATCTTTCA GGACGAGTAC GAGGAGAAGC CCGAGATCGG CAACAAGAGT 
CCTGTCATAC TCAAGGACGA GAAGCCGCCG CTGCTGCCAC CATCCTACAA TACCTCAAAC 
ATGAATGGTG ACAACGACGT GGACGATTAT GTGCCACCAC CATCGGTGGT TGTGGGCGGC 
CGGGAAGTGC GTGGCAAGTC GCCAGCCACG CCCTCCTACC GCAAACCACC GCCATATGTG 
TCGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788363.1
CDS	649..4437
Translation	MRFQWFLSASASLSLFLLLDFVVWIHGERDFNVNDSQVPVIEPKDVPAYKQDPYVTELMSCQNTPSEIVLSLLLKKHDWSELTATKRAHVQAKLAKFFAIPKEFISLDSVSKRELRSMHKLAMRKGGKGNKNIETLNRRLGRASFMIGCGPSYFVMGEPIAKQIAHQMKDGTIGALTEENFGLWFIWRKELKSRSNRKRRQSEGSGADEDDYDYGDDDEEVAEPSTEVPPVTTHAHRHHHGALEVSEKIVSPESVSSSAVPLVPDVEEEIEESVSKLESVISKTIENTKNIKELPVLGEADDGEDEEEVELQQLGVPLAAEIVPDETTSAAATRATEMVKPVDLDEQEISVDSGAVPAIDVEASATPTPSMSARETQKPFSPYDATSSVSSSFSPVASIASGSEAGEDSTSVSTPHLSPANSPTVMPTELDRNGLATTSSSSSSSASPPPSSSVPTQATQPTTSASSFSSSSSTTTTSTTATVSTTTTASSTATTTTTTTTLSESPKPNTVLNELELSTQLPIDDFEGTATATQPTGTATATAIASTAAAAAATAAAATATATTANASATTAGESEFHIESTTHRTISSKEGGEPDAISSGNNSLANNELSTPATPTSSVATTTASTPESSSISSNFVSTDYMEPQPEENSPPIIKTRLQKLAVTSGKAFTFHVLPETFYDAEDQGNLRLALTDKDGHELKANSWLQFNADKRELYGLPLDDTVSRWQYRLSATDSGNASVTETVEISVQQHRAVRTINHEISVFVRINEKPGHNIDWQLKLINAVARTLDDSTNSAVVVREIRQTPHDPHSATFVYFNETLPTSECPEKELKDIIARLDANRLSDLVQPQLGIKSITGQLIGSCQKDLTQVKPTQHMTKNVPPMPRNQVDRVNASLGQLLVYKVPADTFYDANDNQLTLTLKTRDHLELSPRHWLQFDSKNEEFYGIPKSGDIGSEEYLLVAEDSGGLSAHDALVVVVSPAPKRDFGFFFKAYLSIKHERFNADLQRKFVERVAKLNGDPTTGQIQIRSITTHHDSDGTIVNFYNTTLYRKHNSCREKEVAMTRSVYLNSDLSLREAAKRALGPELNLTNFSVVPFSICHHTENIDTNQLDYIPSRPEEPTHKSSFGEDYMITFVWPIVIIVAMLVAASIIACCLHWCRQRSGKMELGDEEERKSFRAKGIPVIFQDEYEEKPEIGNKSPVILKDEKPPLLPPSYNTSNMNGDNDVDDYVPPPSVVVGGREVRGKSPATPSYRKPPPYVSP

Target ORF information:

RefSeq Version	NM_176183.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dystroglycan, transcript variant D (Dg), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176183.3

ORF Insert Sequence:

ATGAGATTCC AGTGGTTCTT ATCGGCCAGT GCGAGCTTGT CGCTGTTCCT TCTCCTGGAC 
TTCGTCGTAT GGATTCACGG CGAGCGGGAC TTCAATGTCA ACGACTCCCA GGTCCCTGTA 
ATTGAGCCAA AGGATGTGCC CGCCTACAAA CAGGATCCGT ATGTTACGGA ATTGATGAGT 
TGCCAAAATA CTCCGAGTGA AATTGTGCTT TCACTTTTGT TGAAGAAGCA CGACTGGAGT 
GAGCTGACGG CCACGAAACG AGCTCATGTC CAGGCCAAGT TGGCCAAGTT CTTTGCCATA 
CCCAAGGAAT TCATTTCCCT GGATTCGGTG TCTAAACGCG AACTGAGATC CATGCACAAG 
TTGGCCATGA GAAAAGGAGG CAAGGGCAAC AAGAATATCG AGACCCTGAA TCGTCGCCTG 
GGACGCGCCA GTTTCATGAT CGGCTGTGGT CCAAGCTACT TTGTGATGGG CGAACCGATA 
GCCAAGCAGA TTGCCCACCA AATGAAGGAT GGCACCATTG GCGCTTTGAC GGAGGAGAAC 
TTCGGGCTGT GGTTCATTTG GCGAAAGGAA TTGAAATCAA GATCAAACCG CAAGCGACGT 
CAATCCGAGG GCTCTGGTGC CGATGAAGAT GACTACGACT ATGGGGATGA CGATGAGGAA 
GTTGCTGAGC CCAGTACGGA AGTGCCACCA GTGACCACAC ATGCCCATCG ACATCATCAC 
GGAGCGTTGG AGGTGTCGGA GAAGATAGTG TCGCCCGAAT CCGTCTCTTC GTCGGCTGTT 
CCCCTGGTTC CGGACGTGGA GGAGGAGATC GAAGAGAGCG TCTCCAAGCT GGAGTCCGTC 
ATCAGCAAGA CGATTGAGAA TACGAAGAAT ATTAAGGAGC TGCCCGTTCT GGGCGAAGCC 
GACGATGGTG AAGATGAAGA GGAAGTGGAA CTACAGCAAT TGGGAGTTCC ATTGGCCGCG 
GAGATTGTGC CAGACGAAAC CACCAGTGCG GCGGCCACAC GTGCCACGGA AATGGTAAAG 
CCCGTTGATT TGGATGAGCA AGAAATTTCG GTGGACTCCG GCGCTGTGCC GGCTATCGAT 
GTGGAGGCCT CGGCCACGCC CACGCCCTCG ATGTCCGCTC GCGAAACACA GAAGCCCTTT 
TCACCCTATG ACGCCACCTC TTCGGTGTCG TCCTCGTTCT CGCCCGTCGC ATCGATTGCA 
TCTGGCAGCG AAGCTGGTGA AGATTCAACT TCGGTGTCGA CCCCTCACCT GTCCCCAGCT 
AACTCACCCA CGGTCATGCC CACAGAGCTA GACAGAAATG GCCTAGCCAC CACATCATCC 
TCATCATCTT CATCCGCCTC ACCTCCTCCA TCGTCATCCG TTCCAACACA AGCCACTCAA 
CCAACCACAT CTGCATCATC ATTCTCATCA TCATCATCAA CAACAACAAC AAGCACAACA 
GCAACAGTCT CAACAACAAC AACAGCATCA TCAACAGCAA CAACAACTAC AACAACAACA 
ACACTGTCTG AATCGCCAAA GCCAAATACT GTGCTTAATG AGCTCGAGCT GAGCACCCAG 
CTGCCCATCG ACGATTTTGA AGGCACTGCT ACTGCAACAC AGCCAACTGG CACTGCAACT 
GCAACTGCCA TTGCAAGCAC AGCAGCAGCA GCAGCAGCAA CAGCAGCAGC AGCAACCGCA 
ACAGCCACAA CTGCCAATGC ATCTGCAACA ACAGCAGGCG AAAGCGAATT TCATATAGAG 
TCCACAACGC ACCGCACGAT TAGCTCTAAG GAAGGAGGAG AACCCGATGC CATCAGCAGC 
GGCAACAATA GCCTGGCCAA TAATGAGCTG TCGACGCCCG CCACGCCCAC ATCTTCGGTG 
GCCACGACCA CGGCATCTAC TCCGGAGTCG AGCAGCATCA GCAGCAACTT TGTCTCCACC 
GACTACATGG AACCGCAGCC GGAAGAGAAT AGTCCGCCCA TCATCAAGAC ACGCCTGCAG 
AAGCTGGCTG TCACATCGGG CAAGGCCTTC ACCTTCCATG TGCTACCGGA AACGTTTTAC 
GATGCCGAGG ATCAGGGTAA TCTCCGCCTG GCGCTCACCG ACAAGGATGG CCACGAGCTA 
AAGGCGAACT CTTGGCTGCA GTTTAACGCC GACAAGAGGG AGCTTTATGG CCTACCTCTG 
GACGACACGG TATCCCGCTG GCAATATCGC CTGTCGGCCA CGGATTCGGG CAACGCCAGT 
GTCACGGAAA CCGTGGAGAT AAGTGTGCAG CAGCATCGAG CGGTTAGGAC CATTAACCAC 
GAGATCAGTG TTTTTGTACG GATCAACGAG AAGCCGGGTC ACAACATTGA TTGGCAGTTG 
AAGCTCATTA ATGCGGTGGC CAGAACCTTG GATGACTCCA CCAATTCGGC AGTGGTGGTG 
CGTGAGATCC GACAGACACC ACACGATCCA CACAGTGCCA CCTTCGTGTA TTTTAATGAA 
ACGCTGCCCA CCAGCGAGTG TCCCGAAAAG GAACTAAAGG ATATTATTGC ACGACTGGAT 
GCCAATAGAC TGAGTGATTT GGTTCAGCCA CAGCTGGGAA TCAAATCTAT AACAGGACAG 
TTAATCGGAT CCTGTCAGAA GGATTTAACT CAGGTGAAGC CCACGCAACA CATGACCAAG 
AATGTGCCTC CAATGCCACG CAACCAGGTG GATCGCGTTA ACGCTAGCTT GGGCCAGCTG 
CTGGTCTACA AAGTGCCGGC GGATACCTTC TATGATGCGA ACGACAATCA ATTGACTCTT 
ACGTTGAAAA CCAGGGATCA CCTTGAGCTT AGCCCACGCC ACTGGCTGCA GTTCGACTCC 
AAGAACGAAG AGTTCTATGG CATTCCGAAA AGCGGTGACA TTGGTTCCGA GGAATATCTG 
TTGGTCGCCG AAGACAGTGG TGGCCTGAGT GCCCATGATG CCCTGGTTGT AGTGGTTAGC 
CCAGCGCCAA AGCGTGACTT TGGGTTCTTC TTCAAAGCGT ATCTGTCTAT CAAACACGAG 
CGATTCAATG CCGATCTGCA GCGGAAGTTT GTAGAGCGTG TAGCGAAGCT GAATGGAGAT 
CCTACTACTG GCCAGATCCA GATCCGTTCG ATAACCACGC ACCACGACTC CGATGGCACC 
ATAGTGAACT TCTACAATAC GACGCTGTAC AGGAAGCACA ACAGTTGTCG GGAGAAGGAA 
GTGGCCATGA CTAGGAGTGT CTACCTAAAC AGTGACCTCA GCCTGAGGGA GGCAGCCAAA 
CGGGCTCTGG GACCCGAGCT CAATTTGACC AACTTTTCGG TGGTGCCCTT CAGTATTTGT 
CATCATACTG AGAACATAGA CACCAACCAA CTTGATTATA TACCTAGTCG CCCCGAAGAG 
CCTACACATA AGTCCTCTTT CGGCGAGGAT TACATGATCA CGTTCGTATG GCCCATCGTG 
ATCATTGTGG CGATGCTTGT GGCTGCCTCA ATCATTGCCT GTTGCCTGCA CTGGTGTCGC 
CAAAGGAGCG GCAAAATGGA GCTAGGCGAC GAAGAGGAGC GCAAGTCCTT CCGTGCCAAG 
GGTATTCCCG TCATCTTTCA GGACGAGTAC GAGGAGAAGC CCGAGATCGG CAACAAGAGT 
CCTGTCATAC TCAAGGACGA GAAGCCGCCG CTGCTGCCAC CATCCTACAA TACCTCAAAC 
ATGAATGGTG ACAACGACGT GGACGATTAT GTGCCACCAC CATCGGTGGT TGTGGGCGGC 
CGGGAAGTGC GTGGCAAGTC GCCAGCCACG CCCTCCTACC GCAAACCACC GCCATATGTG 
TCGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dg ( NM_079032.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523756.1 Drosophila melanogaster dystroglycan, transcript variant A (Dg), mRNA.

Price & Availability↑

CloneID	OFa10160
Clone ID Related Accession (Same CDS sequence)	NM_079032.4
Accession Version	NM_079032.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dystroglycan, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_079032.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGATTCC AGTGGTTCTT ATCGGCCAGT GCGAGCTTGT CGCTGTTCCT TCTCCTGGAC 
TTCGTCGTAT GGATTCACGG CGAGCGGGAC TTCAATGTCA ACGACTCCCA GGTCCCTGTA 
ATTGAGCCAA AGGATGTGCC CGCCTACAAA CAGGATCCGT ATGTTACGGA ATTGATGAGT 
TGCCAAAATA CTCCGAGTGA AATTGTGCTT TCACTTTTGT TGAAGAAGCA CGACTGGAGT 
GAGCTGACGG CCACGAAACG AGCTCATGTC CAGGCCAAGT TGGCCAAGTT CTTTGCCATA 
CCCAAGGAAT TCATTTCCCT GGATTCGGTG TCTAAACGCG AACTGAGATC CATGCACAAG 
TTGGCCATGA GAAAAGGAGG CAAGGGCAAC AAGAATATCG AGACCCTGAA TCGTCGCCTG 
GGACGCGCCA GTTTCATGAT CGGCTGTGGT CCAAGCTACT TTGTGATGGG CGAACCGATA 
GCCAAGCAGA TTGCCCACCA AATGAAGGAT GGCACCATTG GCGCTTTGAC GGAGGAGAAC 
TTCGGGCTGT GGTTCATTTG GCGAAAGGAA TTGAAATCAA GATCAAACCG CAAGCGACGT 
CAATCCGAGG GCTCTGGTGC CGATGAAGAT GACTACGACT ATGGGGATGA CGATGAGGAA 
GTTGCTGAGC CCAGTACGGA AGTGCCACCA GTGACCACAC ATGCCCATCG ACATCATCAC 
GGAGCGCCAA ATACTGTGCT TAATGAGCTC GAGCTGAGCA CCCAGCTGCC CATCGACGAT 
TTTGAAGGCA CTGCTACTGC AACACAGCCA ACTGGCACTG CAACTGCAAC TGCCATTGCA 
AGCACAGCAG CAGCAGCAGC AGCAACAGCA GCAGCAGCAA CCGCAACAGC CACAACTGCC 
AATGCATCTG CAACAACAGC AGGCGAAAGC GAATTTCATA TAGAGTCCAC AACGCACCGC 
ACGATTAGCT CTAAGGAAGG AGGAGAACCC GATGCCATCA GCAGCGGCAA CAATAGCCTG 
GCCAATAATG AGCTGTCGAC GCCCGCCACG CCCACATCTT CGGTGGCCAC GACCACGGCA 
TCTACTCCGG AGTCGAGCAG CATCAGCAGC AACTTTGTCT CCACCGACTA CATGGAACCG 
CAGCCGGAAG AGAATAGTCC GCCCATCATC AAGACACGCC TGCAGAAGCT GGCTGTCACA 
TCGGGCAAGG CCTTCACCTT CCATGTGCTA CCGGAAACGT TTTACGATGC CGAGGATCAG 
GGTAATCTCC GCCTGGCGCT CACCGACAAG GATGGCCACG AGCTAAAGGC GAACTCTTGG 
CTGCAGTTTA ACGCCGACAA GAGGGAGCTT TATGGCCTAC CTCTGGACGA CACGGTATCC 
CGCTGGCAAT ATCGCCTGTC GGCCACGGAT TCGGGCAACG CCAGTGTCAC GGAAACCGTG 
GAGATAAGTG TGCAGCAGCA TCGAGCGGTT AGGACCATTA ACCACGAGAT CAGTGTTTTT 
GTACGGATCA ACGAGAAGCC GGGTCACAAC ATTGATTGGC AGTTGAAGCT CATTAATGCG 
GTGGCCAGAA CCTTGGATGA CTCCACCAAT TCGGCAGTGG TGGTGCGTGA GATCCGACAG 
ACACCACACG ATCCACACAG TGCCACCTTC GTGTATTTTA ATGAAACGCT GCCCACCAGC 
GAGTGTCCCG AAAAGGAACT AAAGGATATT ATTGCACGAC TGGATGCCAA TAGACTGAGT 
GATTTGGTTC AGCCACAGCT GGGAATCAAA TCTATAACAG GACAGTTAAT CGGATCCTGT 
CAGAAGGATT TAACTCAGGT GAAGCCCACG CAACACATGA CCAAGAATGT GCCTCCAATG 
CCACGCAACC AGGTGGATCG CGTTAACGCT AGCTTGGGCC AGCTGCTGGT CTACAAAGTG 
CCGGCGGATA CCTTCTATGA TGCGAACGAC AATCAATTGA CTCTTACGTT GAAAACCAGG 
GATCACCTTG AGCTTAGCCC ACGCCACTGG CTGCAGTTCG ACTCCAAGAA CGAAGAGTTC 
TATGGCATTC CGAAAAGCGG TGACATTGGT TCCGAGGAAT ATCTGTTGGT CGCCGAAGAC 
AGTGGTGGCC TGAGTGCCCA TGATGCCCTG GTTGTAGTGG TTAGCCCAGC GCCAAAGCGT 
GACTTTGGGT TCTTCTTCAA AGCGTATCTG TCTATCAAAC ACGAGCGATT CAATGCCGAT 
CTGCAGCGGA AGTTTGTAGA GCGTGTAGCG AAGCTGAATG GAGATCCTAC TACTGGCCAG 
ATCCAGATCC GTTCGATAAC CACGCACCAC GACTCCGATG GCACCATAGT GAACTTCTAC 
AATACGACGC TGTACAGGAA GCACAACAGT TGTCGGGAGA AGGAAGTGGC CATGACTAGG 
AGTGTCTACC TAAACAGTGA CCTCAGCCTG AGGGAGGCAG CCAAACGGGC TCTGGGACCC 
GAGCTCAATT TGACCAACTT TTCGGTGGTG CCCTTCAGTA TTTGTCATCA TACTGAGAAC 
ATAGACACCA ACCAACTTGA TTATATACCT AGTCGCCCCG AAGAGCCTAC ACATAAGTCC 
TCTTTCGGCG AGGATTACAT GATCACGTTC GTATGGCCCA TCGTGATCAT TGTGGCGATG 
CTTGTGGCTG CCTCAATCAT TGCCTGTTGC CTGCACTGGT GTCGCCAAAG GAGCGGCAAA 
ATGGAGCTAG GCGACGAAGA GGAGCGCAAG TCCTTCCGTG CCAAGGGTAT TCCCGTCATC 
TTTCAGGACG AGTACGAGGA GAAGCCCGAG ATCGGCAACA AGAGTCCTGT CATACTCAAG 
GACGAGAAGC CGCCGCTGCT GCCACCATCC TACAATACCT CAAACATGAA TGGTGACAAC 
GACGTGGACG ATTATGTGCC ACCACCATCG GTGGTTGTGG GCGGCCGGGA AGTGCGTGGC 
AAGTCGCCAG CCACGCCCTC CTACCGCAAA CCACCGCCAT ATGTGTCGCC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523756.1
CDS	649..3642
Translation	MRFQWFLSASASLSLFLLLDFVVWIHGERDFNVNDSQVPVIEPKDVPAYKQDPYVTELMSCQNTPSEIVLSLLLKKHDWSELTATKRAHVQAKLAKFFAIPKEFISLDSVSKRELRSMHKLAMRKGGKGNKNIETLNRRLGRASFMIGCGPSYFVMGEPIAKQIAHQMKDGTIGALTEENFGLWFIWRKELKSRSNRKRRQSEGSGADEDDYDYGDDDEEVAEPSTEVPPVTTHAHRHHHGAPNTVLNELELSTQLPIDDFEGTATATQPTGTATATAIASTAAAAAATAAAATATATTANASATTAGESEFHIESTTHRTISSKEGGEPDAISSGNNSLANNELSTPATPTSSVATTTASTPESSSISSNFVSTDYMEPQPEENSPPIIKTRLQKLAVTSGKAFTFHVLPETFYDAEDQGNLRLALTDKDGHELKANSWLQFNADKRELYGLPLDDTVSRWQYRLSATDSGNASVTETVEISVQQHRAVRTINHEISVFVRINEKPGHNIDWQLKLINAVARTLDDSTNSAVVVREIRQTPHDPHSATFVYFNETLPTSECPEKELKDIIARLDANRLSDLVQPQLGIKSITGQLIGSCQKDLTQVKPTQHMTKNVPPMPRNQVDRVNASLGQLLVYKVPADTFYDANDNQLTLTLKTRDHLELSPRHWLQFDSKNEEFYGIPKSGDIGSEEYLLVAEDSGGLSAHDALVVVVSPAPKRDFGFFFKAYLSIKHERFNADLQRKFVERVAKLNGDPTTGQIQIRSITTHHDSDGTIVNFYNTTLYRKHNSCREKEVAMTRSVYLNSDLSLREAAKRALGPELNLTNFSVVPFSICHHTENIDTNQLDYIPSRPEEPTHKSSFGEDYMITFVWPIVIIVAMLVAASIIACCLHWCRQRSGKMELGDEEERKSFRAKGIPVIFQDEYEEKPEIGNKSPVILKDEKPPLLPPSYNTSNMNGDNDVDDYVPPPSVVVGGREVRGKSPATPSYRKPPPYVSP

Target ORF information:

RefSeq Version	NM_079032.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dystroglycan, transcript variant A (Dg), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079032.4

ORF Insert Sequence:

ATGAGATTCC AGTGGTTCTT ATCGGCCAGT GCGAGCTTGT CGCTGTTCCT TCTCCTGGAC 
TTCGTCGTAT GGATTCACGG CGAGCGGGAC TTCAATGTCA ACGACTCCCA GGTCCCTGTA 
ATTGAGCCAA AGGATGTGCC CGCCTACAAA CAGGATCCGT ATGTTACGGA ATTGATGAGT 
TGCCAAAATA CTCCGAGTGA AATTGTGCTT TCACTTTTGT TGAAGAAGCA CGACTGGAGT 
GAGCTGACGG CCACGAAACG AGCTCATGTC CAGGCCAAGT TGGCCAAGTT CTTTGCCATA 
CCCAAGGAAT TCATTTCCCT GGATTCGGTG TCTAAACGCG AACTGAGATC CATGCACAAG 
TTGGCCATGA GAAAAGGAGG CAAGGGCAAC AAGAATATCG AGACCCTGAA TCGTCGCCTG 
GGACGCGCCA GTTTCATGAT CGGCTGTGGT CCAAGCTACT TTGTGATGGG CGAACCGATA 
GCCAAGCAGA TTGCCCACCA AATGAAGGAT GGCACCATTG GCGCTTTGAC GGAGGAGAAC 
TTCGGGCTGT GGTTCATTTG GCGAAAGGAA TTGAAATCAA GATCAAACCG CAAGCGACGT 
CAATCCGAGG GCTCTGGTGC CGATGAAGAT GACTACGACT ATGGGGATGA CGATGAGGAA 
GTTGCTGAGC CCAGTACGGA AGTGCCACCA GTGACCACAC ATGCCCATCG ACATCATCAC 
GGAGCGCCAA ATACTGTGCT TAATGAGCTC GAGCTGAGCA CCCAGCTGCC CATCGACGAT 
TTTGAAGGCA CTGCTACTGC AACACAGCCA ACTGGCACTG CAACTGCAAC TGCCATTGCA 
AGCACAGCAG CAGCAGCAGC AGCAACAGCA GCAGCAGCAA CCGCAACAGC CACAACTGCC 
AATGCATCTG CAACAACAGC AGGCGAAAGC GAATTTCATA TAGAGTCCAC AACGCACCGC 
ACGATTAGCT CTAAGGAAGG AGGAGAACCC GATGCCATCA GCAGCGGCAA CAATAGCCTG 
GCCAATAATG AGCTGTCGAC GCCCGCCACG CCCACATCTT CGGTGGCCAC GACCACGGCA 
TCTACTCCGG AGTCGAGCAG CATCAGCAGC AACTTTGTCT CCACCGACTA CATGGAACCG 
CAGCCGGAAG AGAATAGTCC GCCCATCATC AAGACACGCC TGCAGAAGCT GGCTGTCACA 
TCGGGCAAGG CCTTCACCTT CCATGTGCTA CCGGAAACGT TTTACGATGC CGAGGATCAG 
GGTAATCTCC GCCTGGCGCT CACCGACAAG GATGGCCACG AGCTAAAGGC GAACTCTTGG 
CTGCAGTTTA ACGCCGACAA GAGGGAGCTT TATGGCCTAC CTCTGGACGA CACGGTATCC 
CGCTGGCAAT ATCGCCTGTC GGCCACGGAT TCGGGCAACG CCAGTGTCAC GGAAACCGTG 
GAGATAAGTG TGCAGCAGCA TCGAGCGGTT AGGACCATTA ACCACGAGAT CAGTGTTTTT 
GTACGGATCA ACGAGAAGCC GGGTCACAAC ATTGATTGGC AGTTGAAGCT CATTAATGCG 
GTGGCCAGAA CCTTGGATGA CTCCACCAAT TCGGCAGTGG TGGTGCGTGA GATCCGACAG 
ACACCACACG ATCCACACAG TGCCACCTTC GTGTATTTTA ATGAAACGCT GCCCACCAGC 
GAGTGTCCCG AAAAGGAACT AAAGGATATT ATTGCACGAC TGGATGCCAA TAGACTGAGT 
GATTTGGTTC AGCCACAGCT GGGAATCAAA TCTATAACAG GACAGTTAAT CGGATCCTGT 
CAGAAGGATT TAACTCAGGT GAAGCCCACG CAACACATGA CCAAGAATGT GCCTCCAATG 
CCACGCAACC AGGTGGATCG CGTTAACGCT AGCTTGGGCC AGCTGCTGGT CTACAAAGTG 
CCGGCGGATA CCTTCTATGA TGCGAACGAC AATCAATTGA CTCTTACGTT GAAAACCAGG 
GATCACCTTG AGCTTAGCCC ACGCCACTGG CTGCAGTTCG ACTCCAAGAA CGAAGAGTTC 
TATGGCATTC CGAAAAGCGG TGACATTGGT TCCGAGGAAT ATCTGTTGGT CGCCGAAGAC 
AGTGGTGGCC TGAGTGCCCA TGATGCCCTG GTTGTAGTGG TTAGCCCAGC GCCAAAGCGT 
GACTTTGGGT TCTTCTTCAA AGCGTATCTG TCTATCAAAC ACGAGCGATT CAATGCCGAT 
CTGCAGCGGA AGTTTGTAGA GCGTGTAGCG AAGCTGAATG GAGATCCTAC TACTGGCCAG 
ATCCAGATCC GTTCGATAAC CACGCACCAC GACTCCGATG GCACCATAGT GAACTTCTAC 
AATACGACGC TGTACAGGAA GCACAACAGT TGTCGGGAGA AGGAAGTGGC CATGACTAGG 
AGTGTCTACC TAAACAGTGA CCTCAGCCTG AGGGAGGCAG CCAAACGGGC TCTGGGACCC 
GAGCTCAATT TGACCAACTT TTCGGTGGTG CCCTTCAGTA TTTGTCATCA TACTGAGAAC 
ATAGACACCA ACCAACTTGA TTATATACCT AGTCGCCCCG AAGAGCCTAC ACATAAGTCC 
TCTTTCGGCG AGGATTACAT GATCACGTTC GTATGGCCCA TCGTGATCAT TGTGGCGATG 
CTTGTGGCTG CCTCAATCAT TGCCTGTTGC CTGCACTGGT GTCGCCAAAG GAGCGGCAAA 
ATGGAGCTAG GCGACGAAGA GGAGCGCAAG TCCTTCCGTG CCAAGGGTAT TCCCGTCATC 
TTTCAGGACG AGTACGAGGA GAAGCCCGAG ATCGGCAACA AGAGTCCTGT CATACTCAAG 
GACGAGAAGC CGCCGCTGCT GCCACCATCC TACAATACCT CAAACATGAA TGGTGACAAC 
GACGTGGACG ATTATGTGCC ACCACCATCG GTGGTTGTGG GCGGCCGGGA AGTGCGTGGC 
AAGTCGCCAG CCACGCCCTC CTACCGCAAA CCACCGCCAT ATGTGTCGCC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dg ( NM_176184.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788364.2 Drosophila melanogaster dystroglycan, transcript variant C (Dg), mRNA.

Price & Availability↑

CloneID	OFa10161
Clone ID Related Accession (Same CDS sequence)	NM_176184.4
Accession Version	NM_176184.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dystroglycan, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_176184.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGATTCC AGTGGTTCTT ATCGGCCAGT GCGAGCTTGT CGCTGTTCCT TCTCCTGGAC 
TTCGTCGTAT GGATTCACGG CGAGCGGGAC TTCAATGTCA ACGACTCCCA GGTCCCTGTA 
ATTGAGCCAA AGGATGTGCC CGCCTACAAA CAGGATCCGT ATGTTACGGA ATTGATGAGT 
TGCCAAAATA CTCCGAGTGA AATTGTGCTT TCACTTTTGT TGAAGAAGCA CGACTGGAGT 
GAGCTGACGG CCACGAAACG AGCTCATGTC CAGGCCAAGT TGGCCAAGTT CTTTGCCATA 
CCCAAGGAAT TCATTTCCCT GGATTCGGTG TCTAAACGCG AACTGAGATC CATGCACAAG 
TTGGCCATGA GAAAAGGAGG CAAGGGCAAC AAGAATATCG AGACCCTGAA TCGTCGCCTG 
GGACGCGCCA GTTTCATGAT CGGCTGTGGT CCAAGCTACT TTGTGATGGG CGAACCGATA 
GCCAAGCAGA TTGCCCACCA AATGAAGGAT GGCACCATTG GCGCTTTGAC GGAGGAGAAC 
TTCGGGCTGT GGTTCATTTG GCGAAAGGAA TTGAAATCAA GATCAAACCG CAAGCGACGT 
CAATCCGAGG GCTCTGGTGC CGATGAAGAT GACTACGACT ATGGGGATGA CGATGAGGAA 
GTTGCTGAGC CCAGTACGGA AGTGCCACCA GTGACCACAC ATGCCCATCG ACATCATCAC 
GGAGCGTTGG AGGTGTCGGA GAAGATAGTG TCGCCCGAAT CCGTCTCTTC GTCGGCTGTT 
CCCCTGGTTC CGGACGTGGA GGAGGAGATC GAAGAGAGCG TCTCCAAGCT GGAGTCCGTC 
ATCAGCAAGA CGATTGAGAA TACGAAGAAT ATTAAGGAGC TGCCCGTTCT GGGCGAAGCC 
GACGATGGTG AAGATGAAGA GGAAGTGGAA CTACAGCAAT TGGGAGTTCC ATTGGCCGCG 
GAGATTGTGC CAGACGAAAC CACCAGTGCG GCGGCCACAC GTGCCACGGA AATGGTAAAG 
CCCGTTGATT TGGATGAGCA AGAAATTTCG GTGGACTCCG GCGCTGTGCC GGCTATCGAT 
GTGGAGGCCT CGGCCACGCC CACGCCCTCG ATGTCCGCTC GCGAAACACA GAAGCCCTTT 
TCACCCTATG ACGCCACCTC TTCGGTGTCG TCCTCGTTCT CGCCCGTCGC ATCGATTGCA 
TCTGGCAGCG AAGCTGGTGA AGATTCAACT TCGGTGTCGA CCCCTCACCT GTCCCCAGCT 
AACTCACCCA CGGTCATGCC CACAGAGCTA GACAGAAATG GCCTAGCCAC CACATCATCC 
TCATCATCTT CATCCGCCTC ACCTCCTCCA TCGTCATCCG TTCCAACACA AGCCACTCAA 
CCAACCACAT CTGCATCATC ATTCTCATCA TCATCATCAA CAACAACAAC AAGCACAACA 
GCAACAGTCT CAACAACAAC AACAGCATCA TCAACAGCAA CAACAACTAC AACAACAACA 
ACACTGTCTG AATCGCCAAA GGAAGGAGGA GAACCCGATG CCATCAGCAG CGGCAACAAT 
AGCCTGGCCA ATAATGAGCT GTCGACGCCC GCCACGCCCA CATCTTCGGT GGCCACGACC 
ACGGCATCTA CTCCGGAGTC GAGCAGCATC AGCAGCAACT TTGTCTCCAC CGACTACATG 
GAACCGCAGC CGGAAGAGAA TAGTCCGCCC ATCATCAAGA CACGCCTGCA GAAGCTGGCT 
GTCACATCGG GCAAGGCCTT CACCTTCCAT GTGCTACCGG AAACGTTTTA CGATGCCGAG 
GATCAGGGTA ATCTCCGCCT GGCGCTCACC GACAAGGATG GCCACGAGCT AAAGGCGAAC 
TCTTGGCTGC AGTTTAACGC CGACAAGAGG GAGCTTTATG GCCTACCTCT GGACGACACG 
GTATCCCGCT GGCAATATCG CCTGTCGGCC ACGGATTCGG GCAACGCCAG TGTCACGGAA 
ACCGTGGAGA TAAGTGTGCA GCAGCATCGA GCGGTTAGGA CCATTAACCA CGAGATCAGT 
GTTTTTGTAC GGATCAACGA GAAGCCGGGT CACAACATTG ATTGGCAGTT GAAGCTCATT 
AATGCGGTGG CCAGAACCTT GGATGACTCC ACCAATTCGG CAGTGGTGGT GCGTGAGATC 
CGACAGACAC CACACGATCC ACACAGTGCC ACCTTCGTGT ATTTTAATGA AACGCTGCCC 
ACCAGCGAGT GTCCCGAAAA GGAACTAAAG GATATTATTG CACGACTGGA TGCCAATAGA 
CTGAGTGATT TGGTTCAGCC ACAGCTGGGA ATCAAATCTA TAACAGGACA GTTAATCGGA 
TCCTGTCAGA AGGATTTAAC TCAGGTGAAG CCCACGCAAC ACATGACCAA GAATGTGCCT 
CCAATGCCAC GCAACCAGGT GGATCGCGTT AACGCTAGCT TGGGCCAGCT GCTGGTCTAC 
AAAGTGCCGG CGGATACCTT CTATGATGCG AACGACAATC AATTGACTCT TACGTTGAAA 
ACCAGGGATC ACCTTGAGCT TAGCCCACGC CACTGGCTGC AGTTCGACTC CAAGAACGAA 
GAGTTCTATG GCATTCCGAA AAGCGGTGAC ATTGGTTCCG AGGAATATCT GTTGGTCGCC 
GAAGACAGTG GTGGCCTGAG TGCCCATGAT GCCCTGGTTG TAGTGGTTAG CCCAGCGCCA 
AAGCGTGACT TTGGGTTCTT CTTCAAAGCG TATCTGTCTA TCAAACACGA GCGATTCAAT 
GCCGATCTGC AGCGGAAGTT TGTAGAGCGT GTAGCGAAGC TGAATGGAGA TCCTACTACT 
GGCCAGATCC AGATCCGTTC GATAACCACG CACCACGACT CCGATGGCAC CATAGTGAAC 
TTCTACAATA CGACGCTGTA CAGGAAGCAC AACAGTTGTC GGGAGAAGGA AGTGGCCATG 
ACTAGGAGTG TCTACCTAAA CAGTGACCTC AGCCTGAGGG AGGCAGCCAA ACGGGCTCTG 
GGACCCGAGC TCAATTTGAC CAACTTTTCG GTGGTGCCCT TCAGTATTTG TCATCATACT 
GAGAACATAG ACACCAACCA ACTTGATTAT ATACCTAGTC GCCCCGAAGA GCCTACACAT 
AAGTCCTCTT TCGGCGAGGA TTACATGATC ACGTTCGTAT GGCCCATCGT GATCATTGTG 
GCGATGCTTG TGGCTGCCTC AATCATTGCC TGTTGCCTGC ACTGGTGTCG CCAAAGGAGC 
GGCAAAATGG AGCTAGGCGA CGAAGAGGAG CGCAAGTCCT TCCGTGCCAA GGGTATTCCC 
GTCATCTTTC AGGACGAGTA CGAGGAGAAG CCCGAGATCG GCAACAAGAG TCCTGTCATA 
CTCAAGGACG AGAAGCCGCC GCTGCTGCCA CCATCCTACA ATACCTCAAA CATGAATGGT 
GACAACGACG TGGACGATTA TGTGCCACCA CCATCGGTGG TTGTGGGCGG CCGGGAAGTG 
CGTGGCAAGT CGCCAGCCAC GCCCTCCTAC CGCAAACCAC CGCCATATGT GTCGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788364.2
CDS	649..4188
Translation	MRFQWFLSASASLSLFLLLDFVVWIHGERDFNVNDSQVPVIEPKDVPAYKQDPYVTELMSCQNTPSEIVLSLLLKKHDWSELTATKRAHVQAKLAKFFAIPKEFISLDSVSKRELRSMHKLAMRKGGKGNKNIETLNRRLGRASFMIGCGPSYFVMGEPIAKQIAHQMKDGTIGALTEENFGLWFIWRKELKSRSNRKRRQSEGSGADEDDYDYGDDDEEVAEPSTEVPPVTTHAHRHHHGALEVSEKIVSPESVSSSAVPLVPDVEEEIEESVSKLESVISKTIENTKNIKELPVLGEADDGEDEEEVELQQLGVPLAAEIVPDETTSAAATRATEMVKPVDLDEQEISVDSGAVPAIDVEASATPTPSMSARETQKPFSPYDATSSVSSSFSPVASIASGSEAGEDSTSVSTPHLSPANSPTVMPTELDRNGLATTSSSSSSSASPPPSSSVPTQATQPTTSASSFSSSSSTTTTSTTATVSTTTTASSTATTTTTTTTLSESPKEGGEPDAISSGNNSLANNELSTPATPTSSVATTTASTPESSSISSNFVSTDYMEPQPEENSPPIIKTRLQKLAVTSGKAFTFHVLPETFYDAEDQGNLRLALTDKDGHELKANSWLQFNADKRELYGLPLDDTVSRWQYRLSATDSGNASVTETVEISVQQHRAVRTINHEISVFVRINEKPGHNIDWQLKLINAVARTLDDSTNSAVVVREIRQTPHDPHSATFVYFNETLPTSECPEKELKDIIARLDANRLSDLVQPQLGIKSITGQLIGSCQKDLTQVKPTQHMTKNVPPMPRNQVDRVNASLGQLLVYKVPADTFYDANDNQLTLTLKTRDHLELSPRHWLQFDSKNEEFYGIPKSGDIGSEEYLLVAEDSGGLSAHDALVVVVSPAPKRDFGFFFKAYLSIKHERFNADLQRKFVERVAKLNGDPTTGQIQIRSITTHHDSDGTIVNFYNTTLYRKHNSCREKEVAMTRSVYLNSDLSLREAAKRALGPELNLTNFSVVPFSICHHTENIDTNQLDYIPSRPEEPTHKSSFGEDYMITFVWPIVIIVAMLVAASIIACCLHWCRQRSGKMELGDEEERKSFRAKGIPVIFQDEYEEKPEIGNKSPVILKDEKPPLLPPSYNTSNMNGDNDVDDYVPPPSVVVGGREVRGKSPATPSYRKPPPYVSP

Target ORF information:

RefSeq Version	NM_176184.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dystroglycan, transcript variant C (Dg), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176184.4

ORF Insert Sequence:

ATGAGATTCC AGTGGTTCTT ATCGGCCAGT GCGAGCTTGT CGCTGTTCCT TCTCCTGGAC 
TTCGTCGTAT GGATTCACGG CGAGCGGGAC TTCAATGTCA ACGACTCCCA GGTCCCTGTA 
ATTGAGCCAA AGGATGTGCC CGCCTACAAA CAGGATCCGT ATGTTACGGA ATTGATGAGT 
TGCCAAAATA CTCCGAGTGA AATTGTGCTT TCACTTTTGT TGAAGAAGCA CGACTGGAGT 
GAGCTGACGG CCACGAAACG AGCTCATGTC CAGGCCAAGT TGGCCAAGTT CTTTGCCATA 
CCCAAGGAAT TCATTTCCCT GGATTCGGTG TCTAAACGCG AACTGAGATC CATGCACAAG 
TTGGCCATGA GAAAAGGAGG CAAGGGCAAC AAGAATATCG AGACCCTGAA TCGTCGCCTG 
GGACGCGCCA GTTTCATGAT CGGCTGTGGT CCAAGCTACT TTGTGATGGG CGAACCGATA 
GCCAAGCAGA TTGCCCACCA AATGAAGGAT GGCACCATTG GCGCTTTGAC GGAGGAGAAC 
TTCGGGCTGT GGTTCATTTG GCGAAAGGAA TTGAAATCAA GATCAAACCG CAAGCGACGT 
CAATCCGAGG GCTCTGGTGC CGATGAAGAT GACTACGACT ATGGGGATGA CGATGAGGAA 
GTTGCTGAGC CCAGTACGGA AGTGCCACCA GTGACCACAC ATGCCCATCG ACATCATCAC 
GGAGCGTTGG AGGTGTCGGA GAAGATAGTG TCGCCCGAAT CCGTCTCTTC GTCGGCTGTT 
CCCCTGGTTC CGGACGTGGA GGAGGAGATC GAAGAGAGCG TCTCCAAGCT GGAGTCCGTC 
ATCAGCAAGA CGATTGAGAA TACGAAGAAT ATTAAGGAGC TGCCCGTTCT GGGCGAAGCC 
GACGATGGTG AAGATGAAGA GGAAGTGGAA CTACAGCAAT TGGGAGTTCC ATTGGCCGCG 
GAGATTGTGC CAGACGAAAC CACCAGTGCG GCGGCCACAC GTGCCACGGA AATGGTAAAG 
CCCGTTGATT TGGATGAGCA AGAAATTTCG GTGGACTCCG GCGCTGTGCC GGCTATCGAT 
GTGGAGGCCT CGGCCACGCC CACGCCCTCG ATGTCCGCTC GCGAAACACA GAAGCCCTTT 
TCACCCTATG ACGCCACCTC TTCGGTGTCG TCCTCGTTCT CGCCCGTCGC ATCGATTGCA 
TCTGGCAGCG AAGCTGGTGA AGATTCAACT TCGGTGTCGA CCCCTCACCT GTCCCCAGCT 
AACTCACCCA CGGTCATGCC CACAGAGCTA GACAGAAATG GCCTAGCCAC CACATCATCC 
TCATCATCTT CATCCGCCTC ACCTCCTCCA TCGTCATCCG TTCCAACACA AGCCACTCAA 
CCAACCACAT CTGCATCATC ATTCTCATCA TCATCATCAA CAACAACAAC AAGCACAACA 
GCAACAGTCT CAACAACAAC AACAGCATCA TCAACAGCAA CAACAACTAC AACAACAACA 
ACACTGTCTG AATCGCCAAA GGAAGGAGGA GAACCCGATG CCATCAGCAG CGGCAACAAT 
AGCCTGGCCA ATAATGAGCT GTCGACGCCC GCCACGCCCA CATCTTCGGT GGCCACGACC 
ACGGCATCTA CTCCGGAGTC GAGCAGCATC AGCAGCAACT TTGTCTCCAC CGACTACATG 
GAACCGCAGC CGGAAGAGAA TAGTCCGCCC ATCATCAAGA CACGCCTGCA GAAGCTGGCT 
GTCACATCGG GCAAGGCCTT CACCTTCCAT GTGCTACCGG AAACGTTTTA CGATGCCGAG 
GATCAGGGTA ATCTCCGCCT GGCGCTCACC GACAAGGATG GCCACGAGCT AAAGGCGAAC 
TCTTGGCTGC AGTTTAACGC CGACAAGAGG GAGCTTTATG GCCTACCTCT GGACGACACG 
GTATCCCGCT GGCAATATCG CCTGTCGGCC ACGGATTCGG GCAACGCCAG TGTCACGGAA 
ACCGTGGAGA TAAGTGTGCA GCAGCATCGA GCGGTTAGGA CCATTAACCA CGAGATCAGT 
GTTTTTGTAC GGATCAACGA GAAGCCGGGT CACAACATTG ATTGGCAGTT GAAGCTCATT 
AATGCGGTGG CCAGAACCTT GGATGACTCC ACCAATTCGG CAGTGGTGGT GCGTGAGATC 
CGACAGACAC CACACGATCC ACACAGTGCC ACCTTCGTGT ATTTTAATGA AACGCTGCCC 
ACCAGCGAGT GTCCCGAAAA GGAACTAAAG GATATTATTG CACGACTGGA TGCCAATAGA 
CTGAGTGATT TGGTTCAGCC ACAGCTGGGA ATCAAATCTA TAACAGGACA GTTAATCGGA 
TCCTGTCAGA AGGATTTAAC TCAGGTGAAG CCCACGCAAC ACATGACCAA GAATGTGCCT 
CCAATGCCAC GCAACCAGGT GGATCGCGTT AACGCTAGCT TGGGCCAGCT GCTGGTCTAC 
AAAGTGCCGG CGGATACCTT CTATGATGCG AACGACAATC AATTGACTCT TACGTTGAAA 
ACCAGGGATC ACCTTGAGCT TAGCCCACGC CACTGGCTGC AGTTCGACTC CAAGAACGAA 
GAGTTCTATG GCATTCCGAA AAGCGGTGAC ATTGGTTCCG AGGAATATCT GTTGGTCGCC 
GAAGACAGTG GTGGCCTGAG TGCCCATGAT GCCCTGGTTG TAGTGGTTAG CCCAGCGCCA 
AAGCGTGACT TTGGGTTCTT CTTCAAAGCG TATCTGTCTA TCAAACACGA GCGATTCAAT 
GCCGATCTGC AGCGGAAGTT TGTAGAGCGT GTAGCGAAGC TGAATGGAGA TCCTACTACT 
GGCCAGATCC AGATCCGTTC GATAACCACG CACCACGACT CCGATGGCAC CATAGTGAAC 
TTCTACAATA CGACGCTGTA CAGGAAGCAC AACAGTTGTC GGGAGAAGGA AGTGGCCATG 
ACTAGGAGTG TCTACCTAAA CAGTGACCTC AGCCTGAGGG AGGCAGCCAA ACGGGCTCTG 
GGACCCGAGC TCAATTTGAC CAACTTTTCG GTGGTGCCCT TCAGTATTTG TCATCATACT 
GAGAACATAG ACACCAACCA ACTTGATTAT ATACCTAGTC GCCCCGAAGA GCCTACACAT 
AAGTCCTCTT TCGGCGAGGA TTACATGATC ACGTTCGTAT GGCCCATCGT GATCATTGTG 
GCGATGCTTG TGGCTGCCTC AATCATTGCC TGTTGCCTGC ACTGGTGTCG CCAAAGGAGC 
GGCAAAATGG AGCTAGGCGA CGAAGAGGAG CGCAAGTCCT TCCGTGCCAA GGGTATTCCC 
GTCATCTTTC AGGACGAGTA CGAGGAGAAG CCCGAGATCG GCAACAAGAG TCCTGTCATA 
CTCAAGGACG AGAAGCCGCC GCTGCTGCCA CCATCCTACA ATACCTCAAA CATGAATGGT 
GACAACGACG TGGACGATTA TGTGCCACCA CCATCGGTGG TTGTGGGCGG CCGGGAAGTG 
CGTGGCAAGT CGCCAGCCAC GCCCTCCTAC CGCAAACCAC CGCCATATGT GTCGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Dg cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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