MCPH1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	MCPH1
Entrez Gene ID	36272
Full Name	Microcephalin
Synonyms	CG13184,CG30038,CG42572,CG8981,Dmcph1,Dmel\CG42572,Dmel_CG30038,Dmel_CG8981,awol,dMCPH1,mcph1,p91
General protein information	Preferred Names Microcephalin Names CG42572 gene product from transcript CG42572-RD CG42572-PA CG42572-PB CG42572-PC CG42572-PD MCPH1-PA MCPH1-PB MCPH1-PC MCPH1-PD absent without leave microcephalin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 48C5-48C5

mRNA and Protein(s)

mRNA	Protein	Name
NM_165852.2	NP_725088.1	microcephalin, isoform A
NM_136867.3	NP_610711.1	microcephalin, isoform B
NM_001169651.2	NP_001163122.1	microcephalin, isoform C
NM_001169652.2	NP_001163123.1	microcephalin, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study reports that Drosophila MCPH1 is cyclically localised during the cell cycle, co-localising with DNA during interphase, but not with mitotic chromosomes
Title: Microcephalin coordinates mitosis in the syncytial Drosophila embryo.

model proposed in which MCPH1 coordinates the S-M transition in fly embryos: in the absence of mcph1, premature chromosome condensation results in mitotic entry with unreplicated DNA, genomic instability, and Chk2-mediated mitotic arrest
Title: The Drosophila homolog of MCPH1, a human microcephaly gene, is required for genomic stability in the early embryo.

The following MCPH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MCPH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09654	NM_136867.3 Latest version!	Drosophila melanogaster microcephalin, transcript variant B (MCPH1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa09655	NM_165852.2 Latest version!	Drosophila melanogaster microcephalin, transcript variant A (MCPH1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa13285	NM_001169651.2 Latest version!	Drosophila melanogaster microcephalin, transcript variant C (MCPH1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa13284	NM_001169652.2 Latest version!	Drosophila melanogaster microcephalin, transcript variant D (MCPH1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MCPH1 ( NM_136867.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610711.1 Drosophila melanogaster microcephalin, transcript variant B (MCPH1), mRNA.

Price & Availability↑

CloneID	OFa09654
Clone ID Related Accession (Same CDS sequence)	NM_136867.3
Accession Version	NM_136867.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	microcephalin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136867.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAAGT TTCTGTTGCG CAATCCCACG TGGAAGACGA AGACGTACGG ATCGGTCGTG 
GCCAGTTCCG CCGCAGCTGC CAAGAAAACA GCGGCGTCAT CGACCCGCCG ACCGTTGCAC 
GATTCAAATC TGGGCGACTT CAGGTCCAAA GCAGCGGCAC CGTCTCCCAA ATCTCTATCC 
CAGGCCCCGG CAGATGACCA GGCGGCCAGG GATCGGCTGA TGCTCAAGCC GGCATTAGCC 
GTCTCCAAAG CGATCAGCCA GCACCGCTCC GAGTTCCACC AGGACGAGAA CGAGGCGGGA 
GCAGAAGGAT CTGCAGCAAA ACCAGATCCA CCCATCGGAG CAGCCACGCC AACCAGGCAG 
GAAGGACACT GGGCCCGACT GCAGCAAGAT CTCAACAGCC CCAACGCTGC ACTCCGATTA 
CGCGCCATTC GTGCATTAAA AAGCCCCACC AAGTCGGCAT ACAATACCTT CGATGTGCCT 
TTAGCCGAAC AAAGCATCAT CACGATGGAA GAACGCAATC CCCAGGAGCC GCCCTCCCTC 
CCCGAGTTGC TCAAGGACAT AGTCGTATAT GTGGAGGTGC GAACTGGAAA CGACAATCGC 
TCTGAGGGCG TAAAATCAAT TATTGCCAAG ATGGGAGCCC AGGTCAAAGA TCGCCTAACG 
CGAACCACCA CACACGTTGT CTTCAAGGAT GGCCTGCTCT CCACCTATAA AAAGGCCACC 
GAGTGGAATA TACCAGTCGT TTCCATTCTG TGGATTGAAG CTTGCAAGGT GCAGCTGAAA 
ATATGCGATC CCAAGCAATT TCCAATCAGC AATATACGCA TGTACGAGTA TCCCGAGCTC 
TTTGGCAAAA TGCCGCGCGT TAAGTTCATG CAGCCGGACT CCGAGTTGAA TCAGCGACCC 
CGCAAACGCC AAGGAACACC CACATCCTCC AAGGACAACG AGCCAGGATC GACTAACAAA 
CGAGCCCAGA CCGGAACTCC AACAACCACC ACACCAAAGA ATGACATCAG CCGCTTTTTC 
AGAGCCATTA ACCACAATAA GCCATCGGAT GATGAGGCAA TAGAGTCGCC AGCCACCAAA 
CTACTCAACA GGATAAGCAG TGGATGCTAC ACTCCTTTGC CGGCCGGTGG CACTCCCCAG 
CTGATGGGAA CCAATGGTAG TGGAGATGCG GTGGCCCAAA AGGTGCAGGC CCAAAAGAGT 
TTGCAATTTG GAGAGCAGAC GGACAGCAAT ACCCCCCAGG CAAAACCATC GAGCACTTCA 
CGCCCACGGC GGAGCACAGT GGATCCACCG CCGGTAATGA CGCCACCAGT GCGCACCACT 
CGCCGTCGTA GCTGCGTAGC TGAAATCAGC AGTCCTGCTG CAACGGATAC GAGAATGACC 
CGCCGCAGAA GTTCGCTACT CACTTCCACG GTTATTCACG AGGCCGATCC AACTCAAACC 
GCAGTTGCGG AACCCAGAAT GACTCGTCGC AGGAGCTCAT TGCTAGGGAT TGCCAAACAA 
GCAGAAGAAA CCGCCCAAAG GCAAACCATT CAACCCATAA CGGAGGAGCT GGCTCCCGTT 
GAAGAGAACA GGAATTCTAT AATTATGGCC GAGACCAATA TAATCAACAA CACCAATTGT 
CTTGAGCAAT CAATTGAGAT AACTGCCGTG GAGGCAGAAG CTTCTTTTAA AGCGGACAGA 
CGTGGCACGC TCTATGCCTC AGAATTGATG GACATAAGTT CTCCTCGTCG GCGCAGCGTG 
GCCAAGTCGA GCTTAGAGTT GAGGCAGGAA AACTTAGCTC CGAGGGCCAG GCAGTCGCTC 
AGCAACGTGA CTACTCAGAC GTCCTTTTTA GACGTTGAGC AGCAAGTGAC GCCGCTGTTC 
AGCTCCACAC GACTGCCCAC AGGCCAGACT ACAGGAAATC GCAGGCGAAC TATTTTTAAC 
ATGGACATGG ACGTGATCAA CGAGAGCATC GAAAGAATGA ACCAGTCACA TCGAATGTCC 
CTAGCCTTGG CCAATAAGGC GGAGCTGGGC GAGTCTTGCC AAGTCCATGA ACAAGAGCCA 
CCGCAAGCAG AGCCAGTCCC ATCACAAGAG CCCAAAAGGC GGCGCCTTTT CAATCCAAAC 
GACGAGGTGG TCATTTCGCC AGCGATGAGC AACAGAAAGA GTCGTTTGAG CATAACCGGA 
TCCGGATCAT CGAGCTCGGT GCAGAAGCGT CGACGCTCGC TAGCCACTCC CGCAAAGTCT 
TCATCATCAG AGTTGTGTGC CACGACTCCT CTTTCCAAAG CCGTCACAAA CAAGGAGGCA 
GAAAAGTCCA AGAGCAATAC AGAAATATCT GAGGCTTCGT TGGATGCCGT TCCGGAAGTG 
AAGAAATCTA CATCAAACGC CGGGGAAGAG AATGAGAAAC CAAAGGGCAA ATCACGTACA 
CGGATTCGCA CGCTAGTCCA CACCAACATG CACCAGGAGC AGATCAACGT CATCCGCAAG 
GTTGGTAATA CGACGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610711.1
CDS	146..2626
Translation	MDKFLLRNPTWKTKTYGSVVASSAAAAKKTAASSTRRPLHDSNLGDFRSKAAAPSPKSLSQAPADDQAARDRLMLKPALAVSKAISQHRSEFHQDENEAGAEGSAAKPDPPIGAATPTRQEGHWARLQQDLNSPNAALRLRAIRALKSPTKSAYNTFDVPLAEQSIITMEERNPQEPPSLPELLKDIVVYVEVRTGNDNRSEGVKSIIAKMGAQVKDRLTRTTTHVVFKDGLLSTYKKATEWNIPVVSILWIEACKVQLKICDPKQFPISNIRMYEYPELFGKMPRVKFMQPDSELNQRPRKRQGTPTSSKDNEPGSTNKRAQTGTPTTTTPKNDISRFFRAINHNKPSDDEAIESPATKLLNRISSGCYTPLPAGGTPQLMGTNGSGDAVAQKVQAQKSLQFGEQTDSNTPQAKPSSTSRPRRSTVDPPPVMTPPVRTTRRRSCVAEISSPAATDTRMTRRRSSLLTSTVIHEADPTQTAVAEPRMTRRRSSLLGIAKQAEETAQRQTIQPITEELAPVEENRNSIIMAETNIINNTNCLEQSIEITAVEAEASFKADRRGTLYASELMDISSPRRRSVAKSSLELRQENLAPRARQSLSNVTTQTSFLDVEQQVTPLFSSTRLPTGQTTGNRRRTIFNMDMDVINESIERMNQSHRMSLALANKAELGESCQVHEQEPPQAEPVPSQEPKRRRLFNPNDEVVISPAMSNRKSRLSITGSGSSSSVQKRRRSLATPAKSSSSELCATTPLSKAVTNKEAEKSKSNTEISEASLDAVPEVKKSTSNAGEENEKPKGKSRTRIRTLVHTNMHQEQINVIRKVGNTTE

Target ORF information:

RefSeq Version	NM_136867.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster microcephalin, transcript variant B (MCPH1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136867.3

ORF Insert Sequence:

ATGGACAAGT TTCTGTTGCG CAATCCCACG TGGAAGACGA AGACGTACGG ATCGGTCGTG 
GCCAGTTCCG CCGCAGCTGC CAAGAAAACA GCGGCGTCAT CGACCCGCCG ACCGTTGCAC 
GATTCAAATC TGGGCGACTT CAGGTCCAAA GCAGCGGCAC CGTCTCCCAA ATCTCTATCC 
CAGGCCCCGG CAGATGACCA GGCGGCCAGG GATCGGCTGA TGCTCAAGCC GGCATTAGCC 
GTCTCCAAAG CGATCAGCCA GCACCGCTCC GAGTTCCACC AGGACGAGAA CGAGGCGGGA 
GCAGAAGGAT CTGCAGCAAA ACCAGATCCA CCCATCGGAG CAGCCACGCC AACCAGGCAG 
GAAGGACACT GGGCCCGACT GCAGCAAGAT CTCAACAGCC CCAACGCTGC ACTCCGATTA 
CGCGCCATTC GTGCATTAAA AAGCCCCACC AAGTCGGCAT ACAATACCTT CGATGTGCCT 
TTAGCCGAAC AAAGCATCAT CACGATGGAA GAACGCAATC CCCAGGAGCC GCCCTCCCTC 
CCCGAGTTGC TCAAGGACAT AGTCGTATAT GTGGAGGTGC GAACTGGAAA CGACAATCGC 
TCTGAGGGCG TAAAATCAAT TATTGCCAAG ATGGGAGCCC AGGTCAAAGA TCGCCTAACG 
CGAACCACCA CACACGTTGT CTTCAAGGAT GGCCTGCTCT CCACCTATAA AAAGGCCACC 
GAGTGGAATA TACCAGTCGT TTCCATTCTG TGGATTGAAG CTTGCAAGGT GCAGCTGAAA 
ATATGCGATC CCAAGCAATT TCCAATCAGC AATATACGCA TGTACGAGTA TCCCGAGCTC 
TTTGGCAAAA TGCCGCGCGT TAAGTTCATG CAGCCGGACT CCGAGTTGAA TCAGCGACCC 
CGCAAACGCC AAGGAACACC CACATCCTCC AAGGACAACG AGCCAGGATC GACTAACAAA 
CGAGCCCAGA CCGGAACTCC AACAACCACC ACACCAAAGA ATGACATCAG CCGCTTTTTC 
AGAGCCATTA ACCACAATAA GCCATCGGAT GATGAGGCAA TAGAGTCGCC AGCCACCAAA 
CTACTCAACA GGATAAGCAG TGGATGCTAC ACTCCTTTGC CGGCCGGTGG CACTCCCCAG 
CTGATGGGAA CCAATGGTAG TGGAGATGCG GTGGCCCAAA AGGTGCAGGC CCAAAAGAGT 
TTGCAATTTG GAGAGCAGAC GGACAGCAAT ACCCCCCAGG CAAAACCATC GAGCACTTCA 
CGCCCACGGC GGAGCACAGT GGATCCACCG CCGGTAATGA CGCCACCAGT GCGCACCACT 
CGCCGTCGTA GCTGCGTAGC TGAAATCAGC AGTCCTGCTG CAACGGATAC GAGAATGACC 
CGCCGCAGAA GTTCGCTACT CACTTCCACG GTTATTCACG AGGCCGATCC AACTCAAACC 
GCAGTTGCGG AACCCAGAAT GACTCGTCGC AGGAGCTCAT TGCTAGGGAT TGCCAAACAA 
GCAGAAGAAA CCGCCCAAAG GCAAACCATT CAACCCATAA CGGAGGAGCT GGCTCCCGTT 
GAAGAGAACA GGAATTCTAT AATTATGGCC GAGACCAATA TAATCAACAA CACCAATTGT 
CTTGAGCAAT CAATTGAGAT AACTGCCGTG GAGGCAGAAG CTTCTTTTAA AGCGGACAGA 
CGTGGCACGC TCTATGCCTC AGAATTGATG GACATAAGTT CTCCTCGTCG GCGCAGCGTG 
GCCAAGTCGA GCTTAGAGTT GAGGCAGGAA AACTTAGCTC CGAGGGCCAG GCAGTCGCTC 
AGCAACGTGA CTACTCAGAC GTCCTTTTTA GACGTTGAGC AGCAAGTGAC GCCGCTGTTC 
AGCTCCACAC GACTGCCCAC AGGCCAGACT ACAGGAAATC GCAGGCGAAC TATTTTTAAC 
ATGGACATGG ACGTGATCAA CGAGAGCATC GAAAGAATGA ACCAGTCACA TCGAATGTCC 
CTAGCCTTGG CCAATAAGGC GGAGCTGGGC GAGTCTTGCC AAGTCCATGA ACAAGAGCCA 
CCGCAAGCAG AGCCAGTCCC ATCACAAGAG CCCAAAAGGC GGCGCCTTTT CAATCCAAAC 
GACGAGGTGG TCATTTCGCC AGCGATGAGC AACAGAAAGA GTCGTTTGAG CATAACCGGA 
TCCGGATCAT CGAGCTCGGT GCAGAAGCGT CGACGCTCGC TAGCCACTCC CGCAAAGTCT 
TCATCATCAG AGTTGTGTGC CACGACTCCT CTTTCCAAAG CCGTCACAAA CAAGGAGGCA 
GAAAAGTCCA AGAGCAATAC AGAAATATCT GAGGCTTCGT TGGATGCCGT TCCGGAAGTG 
AAGAAATCTA CATCAAACGC CGGGGAAGAG AATGAGAAAC CAAAGGGCAA ATCACGTACA 
CGGATTCGCA CGCTAGTCCA CACCAACATG CACCAGGAGC AGATCAACGT CATCCGCAAG 
GTTGGTAATA CGACGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCPH1 ( NM_165852.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725088.1 Drosophila melanogaster microcephalin, transcript variant A (MCPH1), mRNA.

Price & Availability↑

CloneID	OFa09655
Clone ID Related Accession (Same CDS sequence)	NM_165852.2
Accession Version	NM_165852.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	microcephalin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_165852.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGACAA ACGAACCGAG CATTACTAAC AAGTTGCGTT CCCTCCTGCT AGATGACCAG 
GCGGCCAGGG ATCGGCTGAT GCTCAAGCCG GCATTAGCCG TCTCCAAAGC GATCAGCCAG 
CACCGCTCCG AGTTCCACCA GGACGAGAAC GAGGCGGGAG CAGAAGGATC TGCAGCAAAA 
CCAGATCCAC CCATCGGAGC AGCCACGCCA ACCAGGCAGG AAGGACACTG GGCCCGACTG 
CAGCAAGATC TCAACAGCCC CAACGCTGCA CTCCGATTAC GCGCCATTCG TGCATTAAAA 
AGCCCCACCA AGTCGGCATA CAATACCTTC GATGTGCCTT TAGCCGAACA AAGCATCATC 
ACGATGGAAG AACGCAATCC CCAGGAGCCG CCCTCCCTCC CCGAGTTGCT CAAGGACATA 
GTCGTATATG TGGAGGTGCG AACTGGAAAC GACAATCGCT CTGAGGGCGT AAAATCAATT 
ATTGCCAAGA TGGGAGCCCA GGTCAAAGAT CGCCTAACGC GAACCACCAC ACACGTTGTC 
TTCAAGGATG GCCTGCTCTC CACCTATAAA AAGGCCACCG AGTGGAATAT ACCAGTCGTT 
TCCATTCTGT GGATTGAAGC TTGCAAGGTG CAGCTGAAAA TATGCGATCC CAAGCAATTT 
CCAATCAGCA ATATACGCAT GTACGAGTAT CCCGAGCTCT TTGGCAAAAT GCCGCGCGTT 
AAGTTCATGC AGCCGGACTC CGAGTTGAAT CAGCGACCCC GCAAACGCCA AGGAACACCC 
ACATCCTCCA AGGACAACGA GCCAGGATCG ACTAACAAAC GAGCCCAGAC CGGAACTCCA 
ACAACCACCA CACCAAAGAA TGACATCAGC CGCTTTTTCA GAGCCATTAA CCACAATAAG 
CCATCGGATG ATGAGGCAAT AGAGTCGCCA GCCACCAAAC TACTCAACAG GATAAGCAGT 
GGATGCTACA CTCCTTTGCC GGCCGGTGGC ACTCCCCAGC TGATGGGAAC CAATGGTAGT 
GGAGATGCGG TGGCCCAAAA GGTGCAGGCC CAAAAGAGTT TGCAATTTGG AGAGCAGACG 
GACAGCAATA CCCCCCAGGC AAAACCATCG AGCACTTCAC GCCCACGGCG GAGCACAGTG 
GATCCACCGC CGGTAATGAC GCCACCAGTG CGCACCACTC GCCGTCGTAG CTGCGTAGCT 
GAAATCAGCA GTCCTGCTGC AACGGATACG AGAATGACCC GCCGCAGAAG TTCGCTACTC 
ACTTCCACGG TTATTCACGA GGCCGATCCA ACTCAAACCG CAGTTGCGGA ACCCAGAATG 
ACTCGTCGCA GGAGCTCATT GCTAGGGATT GCCAAACAAG CAGAAGAAAC CGCCCAAAGG 
CAAACCATTC AACCCATAAC GGAGGAGCTG GCTCCCGTTG AAGAGAACAG GAATTCTATA 
ATTATGGCCG AGACCAATAT AATCAACAAC ACCAATTGTC TTGAGCAATC AATTGAGATA 
ACTGCCGTGG AGGCAGAAGC TTCTTTTAAA GCGGACAGAC GTGGCACGCT CTATGCCTCA 
GAATTGATGG ACATAAGTTC TCCTCGTCGG CGCAGCGTGG CCAAGTCGAG CTTAGAGTTG 
AGGCAGGAAA ACTTAGCTCC GAGGGCCAGG CAGTCGCTCA GCAACGTGAC TACTCAGACG 
TCCTTTTTAG ACGTTGAGCA GCAAGTGACG CCGCTGTTCA GCTCCACACG ACTGCCCACA 
GGCCAGACTA CAGGAAATCG CAGGCGAACT ATTTTTAACA TGGACATGGA CGTGATCAAC 
GAGAGCATCG AAAGAATGAA CCAGTCACAT CGAATGTCCC TAGCCTTGGC CAATAAGGCG 
GAGCTGGGCG AGTCTTGCCA AGTCCATGAA CAAGAGCCAC CGCAAGCAGA GCCAGTCCCA 
TCACAAGAGC CCAAAAGGCG GCGCCTTTTC AATCCAAACG ACGAGGTGGT CATTTCGCCA 
GCGATGAGCA ACAGAAAGAG TCGTTTGAGC ATAACCGGAT CCGGATCATC GAGCTCGGTG 
CAGAAGCGTC GACGCTCGCT AGCCACTCCC GCAAAGTCTT CATCATCAGA GTTGTGTGCC 
ACGACTCCTC TTTCCAAAGC CGTCACAAAC AAGGAGGCAG AAAAGTCCAA GAGCAATACA 
GAAATATCTG AGGCTTCGTT GGATGCCGTT CCGGAAGTGA AGAAATCTAC ATCAAACGCC 
GGGGAAGAGA ATGAGAAACC AAAGGGCAAA TCACGTACAC GGATTCGCAC GCTAGTCCAC 
ACCAACATGC ACCAGGAGCA GATCAACGTC ATCCGCAAGG TTGGTAATAC GACGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725088.1
CDS	76..2415
Translation	MSTNEPSITNKLRSLLLDDQAARDRLMLKPALAVSKAISQHRSEFHQDENEAGAEGSAAKPDPPIGAATPTRQEGHWARLQQDLNSPNAALRLRAIRALKSPTKSAYNTFDVPLAEQSIITMEERNPQEPPSLPELLKDIVVYVEVRTGNDNRSEGVKSIIAKMGAQVKDRLTRTTTHVVFKDGLLSTYKKATEWNIPVVSILWIEACKVQLKICDPKQFPISNIRMYEYPELFGKMPRVKFMQPDSELNQRPRKRQGTPTSSKDNEPGSTNKRAQTGTPTTTTPKNDISRFFRAINHNKPSDDEAIESPATKLLNRISSGCYTPLPAGGTPQLMGTNGSGDAVAQKVQAQKSLQFGEQTDSNTPQAKPSSTSRPRRSTVDPPPVMTPPVRTTRRRSCVAEISSPAATDTRMTRRRSSLLTSTVIHEADPTQTAVAEPRMTRRRSSLLGIAKQAEETAQRQTIQPITEELAPVEENRNSIIMAETNIINNTNCLEQSIEITAVEAEASFKADRRGTLYASELMDISSPRRRSVAKSSLELRQENLAPRARQSLSNVTTQTSFLDVEQQVTPLFSSTRLPTGQTTGNRRRTIFNMDMDVINESIERMNQSHRMSLALANKAELGESCQVHEQEPPQAEPVPSQEPKRRRLFNPNDEVVISPAMSNRKSRLSITGSGSSSSVQKRRRSLATPAKSSSSELCATTPLSKAVTNKEAEKSKSNTEISEASLDAVPEVKKSTSNAGEENEKPKGKSRTRIRTLVHTNMHQEQINVIRKVGNTTE

Target ORF information:

RefSeq Version	NM_165852.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster microcephalin, transcript variant A (MCPH1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165852.2

ORF Insert Sequence:

ATGTCGACAA ACGAACCGAG CATTACTAAC AAGTTGCGTT CCCTCCTGCT AGATGACCAG 
GCGGCCAGGG ATCGGCTGAT GCTCAAGCCG GCATTAGCCG TCTCCAAAGC GATCAGCCAG 
CACCGCTCCG AGTTCCACCA GGACGAGAAC GAGGCGGGAG CAGAAGGATC TGCAGCAAAA 
CCAGATCCAC CCATCGGAGC AGCCACGCCA ACCAGGCAGG AAGGACACTG GGCCCGACTG 
CAGCAAGATC TCAACAGCCC CAACGCTGCA CTCCGATTAC GCGCCATTCG TGCATTAAAA 
AGCCCCACCA AGTCGGCATA CAATACCTTC GATGTGCCTT TAGCCGAACA AAGCATCATC 
ACGATGGAAG AACGCAATCC CCAGGAGCCG CCCTCCCTCC CCGAGTTGCT CAAGGACATA 
GTCGTATATG TGGAGGTGCG AACTGGAAAC GACAATCGCT CTGAGGGCGT AAAATCAATT 
ATTGCCAAGA TGGGAGCCCA GGTCAAAGAT CGCCTAACGC GAACCACCAC ACACGTTGTC 
TTCAAGGATG GCCTGCTCTC CACCTATAAA AAGGCCACCG AGTGGAATAT ACCAGTCGTT 
TCCATTCTGT GGATTGAAGC TTGCAAGGTG CAGCTGAAAA TATGCGATCC CAAGCAATTT 
CCAATCAGCA ATATACGCAT GTACGAGTAT CCCGAGCTCT TTGGCAAAAT GCCGCGCGTT 
AAGTTCATGC AGCCGGACTC CGAGTTGAAT CAGCGACCCC GCAAACGCCA AGGAACACCC 
ACATCCTCCA AGGACAACGA GCCAGGATCG ACTAACAAAC GAGCCCAGAC CGGAACTCCA 
ACAACCACCA CACCAAAGAA TGACATCAGC CGCTTTTTCA GAGCCATTAA CCACAATAAG 
CCATCGGATG ATGAGGCAAT AGAGTCGCCA GCCACCAAAC TACTCAACAG GATAAGCAGT 
GGATGCTACA CTCCTTTGCC GGCCGGTGGC ACTCCCCAGC TGATGGGAAC CAATGGTAGT 
GGAGATGCGG TGGCCCAAAA GGTGCAGGCC CAAAAGAGTT TGCAATTTGG AGAGCAGACG 
GACAGCAATA CCCCCCAGGC AAAACCATCG AGCACTTCAC GCCCACGGCG GAGCACAGTG 
GATCCACCGC CGGTAATGAC GCCACCAGTG CGCACCACTC GCCGTCGTAG CTGCGTAGCT 
GAAATCAGCA GTCCTGCTGC AACGGATACG AGAATGACCC GCCGCAGAAG TTCGCTACTC 
ACTTCCACGG TTATTCACGA GGCCGATCCA ACTCAAACCG CAGTTGCGGA ACCCAGAATG 
ACTCGTCGCA GGAGCTCATT GCTAGGGATT GCCAAACAAG CAGAAGAAAC CGCCCAAAGG 
CAAACCATTC AACCCATAAC GGAGGAGCTG GCTCCCGTTG AAGAGAACAG GAATTCTATA 
ATTATGGCCG AGACCAATAT AATCAACAAC ACCAATTGTC TTGAGCAATC AATTGAGATA 
ACTGCCGTGG AGGCAGAAGC TTCTTTTAAA GCGGACAGAC GTGGCACGCT CTATGCCTCA 
GAATTGATGG ACATAAGTTC TCCTCGTCGG CGCAGCGTGG CCAAGTCGAG CTTAGAGTTG 
AGGCAGGAAA ACTTAGCTCC GAGGGCCAGG CAGTCGCTCA GCAACGTGAC TACTCAGACG 
TCCTTTTTAG ACGTTGAGCA GCAAGTGACG CCGCTGTTCA GCTCCACACG ACTGCCCACA 
GGCCAGACTA CAGGAAATCG CAGGCGAACT ATTTTTAACA TGGACATGGA CGTGATCAAC 
GAGAGCATCG AAAGAATGAA CCAGTCACAT CGAATGTCCC TAGCCTTGGC CAATAAGGCG 
GAGCTGGGCG AGTCTTGCCA AGTCCATGAA CAAGAGCCAC CGCAAGCAGA GCCAGTCCCA 
TCACAAGAGC CCAAAAGGCG GCGCCTTTTC AATCCAAACG ACGAGGTGGT CATTTCGCCA 
GCGATGAGCA ACAGAAAGAG TCGTTTGAGC ATAACCGGAT CCGGATCATC GAGCTCGGTG 
CAGAAGCGTC GACGCTCGCT AGCCACTCCC GCAAAGTCTT CATCATCAGA GTTGTGTGCC 
ACGACTCCTC TTTCCAAAGC CGTCACAAAC AAGGAGGCAG AAAAGTCCAA GAGCAATACA 
GAAATATCTG AGGCTTCGTT GGATGCCGTT CCGGAAGTGA AGAAATCTAC ATCAAACGCC 
GGGGAAGAGA ATGAGAAACC AAAGGGCAAA TCACGTACAC GGATTCGCAC GCTAGTCCAC 
ACCAACATGC ACCAGGAGCA GATCAACGTC ATCCGCAAGG TTGGTAATAC GACGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCPH1 ( NM_001169651.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163122.1 Drosophila melanogaster microcephalin, transcript variant C (MCPH1), mRNA.

Price & Availability↑

CloneID	OFa13285
Clone ID Related Accession (Same CDS sequence)	NM_001169651.2
Accession Version	NM_001169651.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	microcephalin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001169651.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGACAA ACGAACCGAG CATTACTAAC AAGTTGCGTT CCCTCCTGCT AGATGACCAG 
GCGGCCAGGG ATCGGCTGAT GCTCAAGCCG GCATTAGCCG TCTCCAAAGC GATCAGCCAG 
CACCGCTCCG AGTTCCACCA GGACGAGAAC GAGGCGGGAG CAGAAGGATC TGCAGCAAAA 
CCAGATCCAC CCATCGGAGC AGCCACGCCA ACCAGGCAGG AAGGACACTG GGCCCGACTG 
CAGCAAGATC TCAACAGCCC CAACGCTGCA CTCCGATTAC GCGCCATTCG TGCATTAAAA 
AGCCCCACCA AGTCGGCATA CAATACCTTC GATGTGCCTT TAGCCGAACA AAGCATCATC 
ACGATGGAAG AACGCAATCC CCAGGAGCCG CCCTCCCTCC CCGAGTTGCT CAAGGACATA 
GTCGTATATG TGGAGGTGCG AACTGGAAAC GACAATCGCT CTGAGGGCGT AAAATCAATT 
ATTGCCAAGA TGGGAGCCCA GGTCAAAGAT CGCCTAACGC GAACCACCAC ACACGTTGTC 
TTCAAGGATG GCCTGCTCTC CACCTATAAA AAGGCCACCG AGTGGAATAT ACCAGTCGTT 
TCCATTCTGT GGATTGAAGC TTGCAAGGTG CAGCTGAAAA TATGCGATCC CAAGCAATTT 
CCAATCAGCA ATATACGCAT GTACGAGTAT CCCGAGCTCT TTGGCAAAAT GCCGCGCGTT 
AAGTTCATGC AGCCGGACTC CGAGTTGAAT CAGCGACCCC GCAAACGCCA AGGAACACCC 
ACATCCTCCA AGGACAACGA GCCAGGATCG ACTAACAAAC GAGCCCAGAC CGGAACTCCA 
ACAACCACCA CACCAAAGAA TGACATCAGC CGCTTTTTCA GAGCCATTAA CCACAATAAG 
CCATCGGATG ATGAGGCAAT AGAGTCGCCA GCCACCAAAC TACTCAACAG GATAAGCAGT 
GGATGCTACA CTCCTTTGCC GGCCGGTGGC ACTCCCCAGC TGATGGGAAC CAATGGTAGT 
GGAGATGCGG TGGCCCAAAA GGTGCAGGCC CAAAAGAGTT TGCAATTTGG AGAGCAGACG 
GACAGCAATA CCCCCCAGGC AAAACCATCG AGCACTTCAC GCCCACGGCG GAGCACAGTG 
GATCCACCGC CGGTAATGAC GCCACCAGTG CGCACCACTC GCCGTCGTAG CTGCGTAGCT 
GAAATCAGCA GTCCTGCTGC AACGGATACG AGAATGACCC GCCGCAGAAG TTCGCTACTC 
ACTTCCACGG TTATTCACGA GGCCGATCCA ACTCAAACCG CAGTTGCGGA ACCCAGAATG 
ACTCGTCGCA GGAGCTCATT GCTAGGGATT GCCAAACAAG CAGAAGAAAC CGCCCAAAGG 
CAAACCATTC AACCCATAAC GGAGGAGCTG GCTCCCGTTG AAGAGAACAG GAATTCTATA 
ATTATGGCCG AGACCAATAT AATCAACAAC ACCAATTGTC TTGAGCAATC AATTGAGATA 
ACTGCCGTGG AGGCAGAAGC TTCTTTTAAA GCGGACAGAC GTGGCACGCT CTATGCCTCA 
GAATTGATGG ACATAAGTTC TCCTCGTCGG CGCAGCGTGG CCAAGTCGAG CTTAGAGTTG 
AGGCAGGAAA ACTTAGCTCC GAGGGCCAGG CAGTCGCTCA GCAACGTGAC TACTCAGACG 
TCCTTTTTAG ACGTTGAGCA GCAAGTGACG CCGCTGTTCA GCTCCACACG ACTGCCCACA 
GGCCAGACTA CAGGAAATCG CAGGCGAACT ATTTTTAACA TGGACATGGA CGTGATCAAC 
GAGAGCATCG AAAGAATGAA CCAGTCACAT CGAATGTCCC TAGCCTTGGC CAATAAGGCG 
GAGCTGGGCG AGTCTTGCCA AGTCCATGAA CAAGAGCCAC CGCAAGCAGA GCCAGTCCCA 
TCACAAGAGC CCAAAAGGCG GCGCCTTTTC AATCCAAACG ACGAGGTGGT CATTTCGCCA 
GCGATGAGCA ACAGAAAGAG TCGTTTGAGC ATAACCGGAT CCGGATCATC GAGCTCGGTG 
CAGAAGCGTC GACGCTCGCT AGCCACTCCC GCAAAGTCTT CATCATCAGA GTTGTGTGCC 
ACGACTCCTC TTTCCAAAGC CGTCACAAAC AAGGAGGCAG AAAAGTCCAA GAGCAATACA 
GAAATATCTG AGGCTTCGTT GGATGCCGTT CCGGAAGTGA AGAAATCTAC ATCAAACGCC 
GGGGAAGAGA ATGAGAAACC AAAGGGCAAA TCACGTACAC GGATTCGCAC GCTAGTCCAC 
ACCAACATGC ACCAGGAGCA GATCAACGTC ATCCGCAAGG CCCTGCGCAA GTTACGCGGC 
ATGCGGCTGG ATCCAACCGT GACCCAGCAC ACCACCCACC TGGTTAGCCT GGAGCCGCGA 
CGTACCCTGA ATCTCCTGCG CGGATTGATG CGCGGAGTCT GGATAGTTAG CTACCAGTGG 
GTGTTGGCCT CCATTCGGGC TGGCAAGTGG ATCAATGAAG AGCCCTATGA GCTGACCAGG 
TTTTCGCGTG CAATCGAGAT TTGTCGTACA GAAAGGCAGG CCTTTGGCGT CCACTACCAG 
TGTGAGTTGT TTCGCTTTAT GGAGCCCTTC TATGTGTCCT CCCTCTGCCG ACCGGTGCAG 
TTCAACAATA TGAAGGAGCT GCTCCTGCTG GGCGGTGCCA CCCTGACCGA GAACCGCTTC 
AAGGCCAAGT ACATCATCGG GGACAAGCGG CGCGCCGAGG ACGATCGCGT CTATCTCGAT 
CCTTACTGGG TGCTGGACAG CATCACGAAT ATGCAAATTC AGCGCTTTGG CAAATATCTT 
ATGAAGAGCG CCATTATCTC GGCCGATGGG ATCCGGTACG AGGATCCTAG GGATCGCGAT 
GAGGACAAAA CACAAAGAAG GCACCTTCAC GACTTCGTCG ATCCACCGTT TGTGCTGGAC 
AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163122.1
CDS	76..3021
Translation	MSTNEPSITNKLRSLLLDDQAARDRLMLKPALAVSKAISQHRSEFHQDENEAGAEGSAAKPDPPIGAATPTRQEGHWARLQQDLNSPNAALRLRAIRALKSPTKSAYNTFDVPLAEQSIITMEERNPQEPPSLPELLKDIVVYVEVRTGNDNRSEGVKSIIAKMGAQVKDRLTRTTTHVVFKDGLLSTYKKATEWNIPVVSILWIEACKVQLKICDPKQFPISNIRMYEYPELFGKMPRVKFMQPDSELNQRPRKRQGTPTSSKDNEPGSTNKRAQTGTPTTTTPKNDISRFFRAINHNKPSDDEAIESPATKLLNRISSGCYTPLPAGGTPQLMGTNGSGDAVAQKVQAQKSLQFGEQTDSNTPQAKPSSTSRPRRSTVDPPPVMTPPVRTTRRRSCVAEISSPAATDTRMTRRRSSLLTSTVIHEADPTQTAVAEPRMTRRRSSLLGIAKQAEETAQRQTIQPITEELAPVEENRNSIIMAETNIINNTNCLEQSIEITAVEAEASFKADRRGTLYASELMDISSPRRRSVAKSSLELRQENLAPRARQSLSNVTTQTSFLDVEQQVTPLFSSTRLPTGQTTGNRRRTIFNMDMDVINESIERMNQSHRMSLALANKAELGESCQVHEQEPPQAEPVPSQEPKRRRLFNPNDEVVISPAMSNRKSRLSITGSGSSSSVQKRRRSLATPAKSSSSELCATTPLSKAVTNKEAEKSKSNTEISEASLDAVPEVKKSTSNAGEENEKPKGKSRTRIRTLVHTNMHQEQINVIRKALRKLRGMRLDPTVTQHTTHLVSLEPRRTLNLLRGLMRGVWIVSYQWVLASIRAGKWINEEPYELTRFSRAIEICRTERQAFGVHYQCELFRFMEPFYVSSLCRPVQFNNMKELLLLGGATLTENRFKAKYIIGDKRRAEDDRVYLDPYWVLDSITNMQIQRFGKYLMKSAIISADGIRYEDPRDRDEDKTQRRHLHDFVDPPFVLDK

Target ORF information:

RefSeq Version	NM_001169651.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster microcephalin, transcript variant C (MCPH1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169651.2

ORF Insert Sequence:

ATGTCGACAA ACGAACCGAG CATTACTAAC AAGTTGCGTT CCCTCCTGCT AGATGACCAG 
GCGGCCAGGG ATCGGCTGAT GCTCAAGCCG GCATTAGCCG TCTCCAAAGC GATCAGCCAG 
CACCGCTCCG AGTTCCACCA GGACGAGAAC GAGGCGGGAG CAGAAGGATC TGCAGCAAAA 
CCAGATCCAC CCATCGGAGC AGCCACGCCA ACCAGGCAGG AAGGACACTG GGCCCGACTG 
CAGCAAGATC TCAACAGCCC CAACGCTGCA CTCCGATTAC GCGCCATTCG TGCATTAAAA 
AGCCCCACCA AGTCGGCATA CAATACCTTC GATGTGCCTT TAGCCGAACA AAGCATCATC 
ACGATGGAAG AACGCAATCC CCAGGAGCCG CCCTCCCTCC CCGAGTTGCT CAAGGACATA 
GTCGTATATG TGGAGGTGCG AACTGGAAAC GACAATCGCT CTGAGGGCGT AAAATCAATT 
ATTGCCAAGA TGGGAGCCCA GGTCAAAGAT CGCCTAACGC GAACCACCAC ACACGTTGTC 
TTCAAGGATG GCCTGCTCTC CACCTATAAA AAGGCCACCG AGTGGAATAT ACCAGTCGTT 
TCCATTCTGT GGATTGAAGC TTGCAAGGTG CAGCTGAAAA TATGCGATCC CAAGCAATTT 
CCAATCAGCA ATATACGCAT GTACGAGTAT CCCGAGCTCT TTGGCAAAAT GCCGCGCGTT 
AAGTTCATGC AGCCGGACTC CGAGTTGAAT CAGCGACCCC GCAAACGCCA AGGAACACCC 
ACATCCTCCA AGGACAACGA GCCAGGATCG ACTAACAAAC GAGCCCAGAC CGGAACTCCA 
ACAACCACCA CACCAAAGAA TGACATCAGC CGCTTTTTCA GAGCCATTAA CCACAATAAG 
CCATCGGATG ATGAGGCAAT AGAGTCGCCA GCCACCAAAC TACTCAACAG GATAAGCAGT 
GGATGCTACA CTCCTTTGCC GGCCGGTGGC ACTCCCCAGC TGATGGGAAC CAATGGTAGT 
GGAGATGCGG TGGCCCAAAA GGTGCAGGCC CAAAAGAGTT TGCAATTTGG AGAGCAGACG 
GACAGCAATA CCCCCCAGGC AAAACCATCG AGCACTTCAC GCCCACGGCG GAGCACAGTG 
GATCCACCGC CGGTAATGAC GCCACCAGTG CGCACCACTC GCCGTCGTAG CTGCGTAGCT 
GAAATCAGCA GTCCTGCTGC AACGGATACG AGAATGACCC GCCGCAGAAG TTCGCTACTC 
ACTTCCACGG TTATTCACGA GGCCGATCCA ACTCAAACCG CAGTTGCGGA ACCCAGAATG 
ACTCGTCGCA GGAGCTCATT GCTAGGGATT GCCAAACAAG CAGAAGAAAC CGCCCAAAGG 
CAAACCATTC AACCCATAAC GGAGGAGCTG GCTCCCGTTG AAGAGAACAG GAATTCTATA 
ATTATGGCCG AGACCAATAT AATCAACAAC ACCAATTGTC TTGAGCAATC AATTGAGATA 
ACTGCCGTGG AGGCAGAAGC TTCTTTTAAA GCGGACAGAC GTGGCACGCT CTATGCCTCA 
GAATTGATGG ACATAAGTTC TCCTCGTCGG CGCAGCGTGG CCAAGTCGAG CTTAGAGTTG 
AGGCAGGAAA ACTTAGCTCC GAGGGCCAGG CAGTCGCTCA GCAACGTGAC TACTCAGACG 
TCCTTTTTAG ACGTTGAGCA GCAAGTGACG CCGCTGTTCA GCTCCACACG ACTGCCCACA 
GGCCAGACTA CAGGAAATCG CAGGCGAACT ATTTTTAACA TGGACATGGA CGTGATCAAC 
GAGAGCATCG AAAGAATGAA CCAGTCACAT CGAATGTCCC TAGCCTTGGC CAATAAGGCG 
GAGCTGGGCG AGTCTTGCCA AGTCCATGAA CAAGAGCCAC CGCAAGCAGA GCCAGTCCCA 
TCACAAGAGC CCAAAAGGCG GCGCCTTTTC AATCCAAACG ACGAGGTGGT CATTTCGCCA 
GCGATGAGCA ACAGAAAGAG TCGTTTGAGC ATAACCGGAT CCGGATCATC GAGCTCGGTG 
CAGAAGCGTC GACGCTCGCT AGCCACTCCC GCAAAGTCTT CATCATCAGA GTTGTGTGCC 
ACGACTCCTC TTTCCAAAGC CGTCACAAAC AAGGAGGCAG AAAAGTCCAA GAGCAATACA 
GAAATATCTG AGGCTTCGTT GGATGCCGTT CCGGAAGTGA AGAAATCTAC ATCAAACGCC 
GGGGAAGAGA ATGAGAAACC AAAGGGCAAA TCACGTACAC GGATTCGCAC GCTAGTCCAC 
ACCAACATGC ACCAGGAGCA GATCAACGTC ATCCGCAAGG CCCTGCGCAA GTTACGCGGC 
ATGCGGCTGG ATCCAACCGT GACCCAGCAC ACCACCCACC TGGTTAGCCT GGAGCCGCGA 
CGTACCCTGA ATCTCCTGCG CGGATTGATG CGCGGAGTCT GGATAGTTAG CTACCAGTGG 
GTGTTGGCCT CCATTCGGGC TGGCAAGTGG ATCAATGAAG AGCCCTATGA GCTGACCAGG 
TTTTCGCGTG CAATCGAGAT TTGTCGTACA GAAAGGCAGG CCTTTGGCGT CCACTACCAG 
TGTGAGTTGT TTCGCTTTAT GGAGCCCTTC TATGTGTCCT CCCTCTGCCG ACCGGTGCAG 
TTCAACAATA TGAAGGAGCT GCTCCTGCTG GGCGGTGCCA CCCTGACCGA GAACCGCTTC 
AAGGCCAAGT ACATCATCGG GGACAAGCGG CGCGCCGAGG ACGATCGCGT CTATCTCGAT 
CCTTACTGGG TGCTGGACAG CATCACGAAT ATGCAAATTC AGCGCTTTGG CAAATATCTT 
ATGAAGAGCG CCATTATCTC GGCCGATGGG ATCCGGTACG AGGATCCTAG GGATCGCGAT 
GAGGACAAAA CACAAAGAAG GCACCTTCAC GACTTCGTCG ATCCACCGTT TGTGCTGGAC 
AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCPH1 ( NM_001169652.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163123.1 Drosophila melanogaster microcephalin, transcript variant D (MCPH1), mRNA.

Price & Availability↑

CloneID	OFa13284
Clone ID Related Accession (Same CDS sequence)	NM_001169652.2
Accession Version	NM_001169652.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	microcephalin, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001169652.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAAGT TTCTGTTGCG CAATCCCACG TGGAAGACGA AGACGTACGG ATCGGTCGTG 
GCCAGTTCCG CCGCAGCTGC CAAGAAAACA GCGGCGTCAT CGACCCGCCG ACCGTTGCAC 
GATTCAAATC TGGGCGACTT CAGGTCCAAA GCAGCGGCAC CGTCTCCCAA ATCTCTATCC 
CAGGCCCCGG CAGATGACCA GGCGGCCAGG GATCGGCTGA TGCTCAAGCC GGCATTAGCC 
GTCTCCAAAG CGATCAGCCA GCACCGCTCC GAGTTCCACC AGGACGAGAA CGAGGCGGGA 
GCAGAAGGAT CTGCAGCAAA ACCAGATCCA CCCATCGGAG CAGCCACGCC AACCAGGCAG 
GAAGGACACT GGGCCCGACT GCAGCAAGAT CTCAACAGCC CCAACGCTGC ACTCCGATTA 
CGCGCCATTC GTGCATTAAA AAGCCCCACC AAGTCGGCAT ACAATACCTT CGATGTGCCT 
TTAGCCGAAC AAAGCATCAT CACGATGGAA GAACGCAATC CCCAGGAGCC GCCCTCCCTC 
CCCGAGTTGC TCAAGGACAT AGTCGTATAT GTGGAGGTGC GAACTGGAAA CGACAATCGC 
TCTGAGGGCG TAAAATCAAT TATTGCCAAG ATGGGAGCCC AGGTCAAAGA TCGCCTAACG 
CGAACCACCA CACACGTTGT CTTCAAGGAT GGCCTGCTCT CCACCTATAA AAAGGCCACC 
GAGTGGAATA TACCAGTCGT TTCCATTCTG TGGATTGAAG CTTGCAAGGT GCAGCTGAAA 
ATATGCGATC CCAAGCAATT TCCAATCAGC AATATACGCA TGTACGAGTA TCCCGAGCTC 
TTTGGCAAAA TGCCGCGCGT TAAGTTCATG CAGCCGGACT CCGAGTTGAA TCAGCGACCC 
CGCAAACGCC AAGGAACACC CACATCCTCC AAGGACAACG AGCCAGGATC GACTAACAAA 
CGAGCCCAGA CCGGAACTCC AACAACCACC ACACCAAAGA ATGACATCAG CCGCTTTTTC 
AGAGCCATTA ACCACAATAA GCCATCGGAT GATGAGGCAA TAGAGTCGCC AGCCACCAAA 
CTACTCAACA GGATAAGCAG TGGATGCTAC ACTCCTTTGC CGGCCGGTGG CACTCCCCAG 
CTGATGGGAA CCAATGGTAG TGGAGATGCG GTGGCCCAAA AGGTGCAGGC CCAAAAGAGT 
TTGCAATTTG GAGAGCAGAC GGACAGCAAT ACCCCCCAGG CAAAACCATC GAGCACTTCA 
CGCCCACGGC GGAGCACAGT GGATCCACCG CCGGTAATGA CGCCACCAGT GCGCACCACT 
CGCCGTCGTA GCTGCGTAGC TGAAATCAGC AGTCCTGCTG CAACGGATAC GAGAATGACC 
CGCCGCAGAA GTTCGCTACT CACTTCCACG GTTATTCACG AGGCCGATCC AACTCAAACC 
GCAGTTGCGG AACCCAGAAT GACTCGTCGC AGGAGCTCAT TGCTAGGGAT TGCCAAACAA 
GCAGAAGAAA CCGCCCAAAG GCAAACCATT CAACCCATAA CGGAGGAGCT GGCTCCCGTT 
GAAGAGAACA GGAATTCTAT AATTATGGCC GAGACCAATA TAATCAACAA CACCAATTGT 
CTTGAGCAAT CAATTGAGAT AACTGCCGTG GAGGCAGAAG CTTCTTTTAA AGCGGACAGA 
CGTGGCACGC TCTATGCCTC AGAATTGATG GACATAAGTT CTCCTCGTCG GCGCAGCGTG 
GCCAAGTCGA GCTTAGAGTT GAGGCAGGAA AACTTAGCTC CGAGGGCCAG GCAGTCGCTC 
AGCAACGTGA CTACTCAGAC GTCCTTTTTA GACGTTGAGC AGCAAGTGAC GCCGCTGTTC 
AGCTCCACAC GACTGCCCAC AGGCCAGACT ACAGGAAATC GCAGGCGAAC TATTTTTAAC 
ATGGACATGG ACGTGATCAA CGAGAGCATC GAAAGAATGA ACCAGTCACA TCGAATGTCC 
CTAGCCTTGG CCAATAAGGC GGAGCTGGGC GAGTCTTGCC AAGTCCATGA ACAAGAGCCA 
CCGCAAGCAG AGCCAGTCCC ATCACAAGAG CCCAAAAGGC GGCGCCTTTT CAATCCAAAC 
GACGAGGTGG TCATTTCGCC AGCGATGAGC AACAGAAAGA GTCGTTTGAG CATAACCGGA 
TCCGGATCAT CGAGCTCGGT GCAGAAGCGT CGACGCTCGC TAGCCACTCC CGCAAAGTCT 
TCATCATCAG AGTTGTGTGC CACGACTCCT CTTTCCAAAG CCGTCACAAA CAAGGAGGCA 
GAAAAGTCCA AGAGCAATAC AGAAATATCT GAGGCTTCGT TGGATGCCGT TCCGGAAGTG 
AAGAAATCTA CATCAAACGC CGGGGAAGAG AATGAGAAAC CAAAGGGCAA ATCACGTACA 
CGGATTCGCA CGCTAGTCCA CACCAACATG CACCAGGAGC AGATCAACGT CATCCGCAAG 
GCCCTGCGCA AGTTACGCGG CATGCGGCTG GATCCAACCG TGACCCAGCA CACCACCCAC 
CTGGTTAGCC TGGAGCCGCG ACGTACCCTG AATCTCCTGC GCGGATTGAT GCGCGGAGTC 
TGGATAGTTA GCTACCAGTG GGTGTTGGCC TCCATTCGGG CTGGCAAGTG GATCAATGAA 
GAGCCCTATG AGCTGACCAG GTTTTCGCGT GCAATCGAGA TTTGTCGTAC AGAAAGGCAG 
GCCTTTGGCG TCCACTACCA GTGTGAGTTG TTTCGCTTTA TGGAGCCCTT CTATGTGTCC 
TCCCTCTGCC GACCGGTGCA GTTCAACAAT ATGAAGGAGC TGCTCCTGCT GGGCGGTGCC 
ACCCTGACCG AGAACCGCTT CAAGGCCAAG TACATCATCG GGGACAAGCG GCGCGCCGAG 
GACGATCGCG TCTATCTCGA TCCTTACTGG GTGCTGGACA GCATCACGAA TATGCAAATT 
CAGCGCTTTG GCAAATATCT TATGAAGAGC GCCATTATCT CGGCCGATGG GATCCGGTAC 
GAGGATCCTA GGGATCGCGA TGAGGACAAA ACACAAAGAA GGCACCTTCA CGACTTCGTC 
GATCCACCGT TTGTGCTGGA CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163123.1
CDS	146..3232
Translation	MDKFLLRNPTWKTKTYGSVVASSAAAAKKTAASSTRRPLHDSNLGDFRSKAAAPSPKSLSQAPADDQAARDRLMLKPALAVSKAISQHRSEFHQDENEAGAEGSAAKPDPPIGAATPTRQEGHWARLQQDLNSPNAALRLRAIRALKSPTKSAYNTFDVPLAEQSIITMEERNPQEPPSLPELLKDIVVYVEVRTGNDNRSEGVKSIIAKMGAQVKDRLTRTTTHVVFKDGLLSTYKKATEWNIPVVSILWIEACKVQLKICDPKQFPISNIRMYEYPELFGKMPRVKFMQPDSELNQRPRKRQGTPTSSKDNEPGSTNKRAQTGTPTTTTPKNDISRFFRAINHNKPSDDEAIESPATKLLNRISSGCYTPLPAGGTPQLMGTNGSGDAVAQKVQAQKSLQFGEQTDSNTPQAKPSSTSRPRRSTVDPPPVMTPPVRTTRRRSCVAEISSPAATDTRMTRRRSSLLTSTVIHEADPTQTAVAEPRMTRRRSSLLGIAKQAEETAQRQTIQPITEELAPVEENRNSIIMAETNIINNTNCLEQSIEITAVEAEASFKADRRGTLYASELMDISSPRRRSVAKSSLELRQENLAPRARQSLSNVTTQTSFLDVEQQVTPLFSSTRLPTGQTTGNRRRTIFNMDMDVINESIERMNQSHRMSLALANKAELGESCQVHEQEPPQAEPVPSQEPKRRRLFNPNDEVVISPAMSNRKSRLSITGSGSSSSVQKRRRSLATPAKSSSSELCATTPLSKAVTNKEAEKSKSNTEISEASLDAVPEVKKSTSNAGEENEKPKGKSRTRIRTLVHTNMHQEQINVIRKALRKLRGMRLDPTVTQHTTHLVSLEPRRTLNLLRGLMRGVWIVSYQWVLASIRAGKWINEEPYELTRFSRAIEICRTERQAFGVHYQCELFRFMEPFYVSSLCRPVQFNNMKELLLLGGATLTENRFKAKYIIGDKRRAEDDRVYLDPYWVLDSITNMQIQRFGKYLMKSAIISADGIRYEDPRDRDEDKTQRRHLHDFVDPPFVLDK

Target ORF information:

RefSeq Version	NM_001169652.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster microcephalin, transcript variant D (MCPH1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169652.2

ORF Insert Sequence:

ATGGACAAGT TTCTGTTGCG CAATCCCACG TGGAAGACGA AGACGTACGG ATCGGTCGTG 
GCCAGTTCCG CCGCAGCTGC CAAGAAAACA GCGGCGTCAT CGACCCGCCG ACCGTTGCAC 
GATTCAAATC TGGGCGACTT CAGGTCCAAA GCAGCGGCAC CGTCTCCCAA ATCTCTATCC 
CAGGCCCCGG CAGATGACCA GGCGGCCAGG GATCGGCTGA TGCTCAAGCC GGCATTAGCC 
GTCTCCAAAG CGATCAGCCA GCACCGCTCC GAGTTCCACC AGGACGAGAA CGAGGCGGGA 
GCAGAAGGAT CTGCAGCAAA ACCAGATCCA CCCATCGGAG CAGCCACGCC AACCAGGCAG 
GAAGGACACT GGGCCCGACT GCAGCAAGAT CTCAACAGCC CCAACGCTGC ACTCCGATTA 
CGCGCCATTC GTGCATTAAA AAGCCCCACC AAGTCGGCAT ACAATACCTT CGATGTGCCT 
TTAGCCGAAC AAAGCATCAT CACGATGGAA GAACGCAATC CCCAGGAGCC GCCCTCCCTC 
CCCGAGTTGC TCAAGGACAT AGTCGTATAT GTGGAGGTGC GAACTGGAAA CGACAATCGC 
TCTGAGGGCG TAAAATCAAT TATTGCCAAG ATGGGAGCCC AGGTCAAAGA TCGCCTAACG 
CGAACCACCA CACACGTTGT CTTCAAGGAT GGCCTGCTCT CCACCTATAA AAAGGCCACC 
GAGTGGAATA TACCAGTCGT TTCCATTCTG TGGATTGAAG CTTGCAAGGT GCAGCTGAAA 
ATATGCGATC CCAAGCAATT TCCAATCAGC AATATACGCA TGTACGAGTA TCCCGAGCTC 
TTTGGCAAAA TGCCGCGCGT TAAGTTCATG CAGCCGGACT CCGAGTTGAA TCAGCGACCC 
CGCAAACGCC AAGGAACACC CACATCCTCC AAGGACAACG AGCCAGGATC GACTAACAAA 
CGAGCCCAGA CCGGAACTCC AACAACCACC ACACCAAAGA ATGACATCAG CCGCTTTTTC 
AGAGCCATTA ACCACAATAA GCCATCGGAT GATGAGGCAA TAGAGTCGCC AGCCACCAAA 
CTACTCAACA GGATAAGCAG TGGATGCTAC ACTCCTTTGC CGGCCGGTGG CACTCCCCAG 
CTGATGGGAA CCAATGGTAG TGGAGATGCG GTGGCCCAAA AGGTGCAGGC CCAAAAGAGT 
TTGCAATTTG GAGAGCAGAC GGACAGCAAT ACCCCCCAGG CAAAACCATC GAGCACTTCA 
CGCCCACGGC GGAGCACAGT GGATCCACCG CCGGTAATGA CGCCACCAGT GCGCACCACT 
CGCCGTCGTA GCTGCGTAGC TGAAATCAGC AGTCCTGCTG CAACGGATAC GAGAATGACC 
CGCCGCAGAA GTTCGCTACT CACTTCCACG GTTATTCACG AGGCCGATCC AACTCAAACC 
GCAGTTGCGG AACCCAGAAT GACTCGTCGC AGGAGCTCAT TGCTAGGGAT TGCCAAACAA 
GCAGAAGAAA CCGCCCAAAG GCAAACCATT CAACCCATAA CGGAGGAGCT GGCTCCCGTT 
GAAGAGAACA GGAATTCTAT AATTATGGCC GAGACCAATA TAATCAACAA CACCAATTGT 
CTTGAGCAAT CAATTGAGAT AACTGCCGTG GAGGCAGAAG CTTCTTTTAA AGCGGACAGA 
CGTGGCACGC TCTATGCCTC AGAATTGATG GACATAAGTT CTCCTCGTCG GCGCAGCGTG 
GCCAAGTCGA GCTTAGAGTT GAGGCAGGAA AACTTAGCTC CGAGGGCCAG GCAGTCGCTC 
AGCAACGTGA CTACTCAGAC GTCCTTTTTA GACGTTGAGC AGCAAGTGAC GCCGCTGTTC 
AGCTCCACAC GACTGCCCAC AGGCCAGACT ACAGGAAATC GCAGGCGAAC TATTTTTAAC 
ATGGACATGG ACGTGATCAA CGAGAGCATC GAAAGAATGA ACCAGTCACA TCGAATGTCC 
CTAGCCTTGG CCAATAAGGC GGAGCTGGGC GAGTCTTGCC AAGTCCATGA ACAAGAGCCA 
CCGCAAGCAG AGCCAGTCCC ATCACAAGAG CCCAAAAGGC GGCGCCTTTT CAATCCAAAC 
GACGAGGTGG TCATTTCGCC AGCGATGAGC AACAGAAAGA GTCGTTTGAG CATAACCGGA 
TCCGGATCAT CGAGCTCGGT GCAGAAGCGT CGACGCTCGC TAGCCACTCC CGCAAAGTCT 
TCATCATCAG AGTTGTGTGC CACGACTCCT CTTTCCAAAG CCGTCACAAA CAAGGAGGCA 
GAAAAGTCCA AGAGCAATAC AGAAATATCT GAGGCTTCGT TGGATGCCGT TCCGGAAGTG 
AAGAAATCTA CATCAAACGC CGGGGAAGAG AATGAGAAAC CAAAGGGCAA ATCACGTACA 
CGGATTCGCA CGCTAGTCCA CACCAACATG CACCAGGAGC AGATCAACGT CATCCGCAAG 
GCCCTGCGCA AGTTACGCGG CATGCGGCTG GATCCAACCG TGACCCAGCA CACCACCCAC 
CTGGTTAGCC TGGAGCCGCG ACGTACCCTG AATCTCCTGC GCGGATTGAT GCGCGGAGTC 
TGGATAGTTA GCTACCAGTG GGTGTTGGCC TCCATTCGGG CTGGCAAGTG GATCAATGAA 
GAGCCCTATG AGCTGACCAG GTTTTCGCGT GCAATCGAGA TTTGTCGTAC AGAAAGGCAG 
GCCTTTGGCG TCCACTACCA GTGTGAGTTG TTTCGCTTTA TGGAGCCCTT CTATGTGTCC 
TCCCTCTGCC GACCGGTGCA GTTCAACAAT ATGAAGGAGC TGCTCCTGCT GGGCGGTGCC 
ACCCTGACCG AGAACCGCTT CAAGGCCAAG TACATCATCG GGGACAAGCG GCGCGCCGAG 
GACGATCGCG TCTATCTCGA TCCTTACTGG GTGCTGGACA GCATCACGAA TATGCAAATT 
CAGCGCTTTG GCAAATATCT TATGAAGAGC GCCATTATCT CGGCCGATGG GATCCGGTAC 
GAGGATCCTA GGGATCGCGA TGAGGACAAA ACACAAAGAA GGCACCTTCA CGACTTCGTC 
GATCCACCGT TTGTGCTGGA CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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MCPH1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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