Pld cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pld
Entrez Gene ID	35554
Full Name	Phospholipase D
Synonyms	CG12110,Dmel\CG12110,PLD,dPLD,dPld,pld,pld1
General protein information	Preferred Names Phospholipase D Names CG12110 gene product from transcript CG12110-RB CG12110-PA CG12110-PB CG12110-PC CG12110-PD CG12110-PF CG12110-PG Pld-PA Pld-PB Pld-PC Pld-PD Pld-PF Pld-PG phospholipase D
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 42A14-42A14

mRNA and Protein(s)

mRNA	Protein	Name
NM_165470.3	NP_724470.2	phospholipase D, isoform B
NM_078903.4	NP_523627.3	phospholipase D, isoform D
NM_165469.4	NP_724469.2	phospholipase D, isoform A
NM_165472.3	NP_724472.3	phospholipase D, isoform G
NM_165471.3	NP_724471.2	phospholipase D, isoform C
NM_001144138.2	NP_001137610.1	phospholipase D, isoform F

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	si:ch211-168k14.2	XP_001921567.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010687	XP_311402.2
Drosophila melanogaster (fruit fly)	Pld	NP_724472.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Phospholipase D activity couples plasma membrane endocytosis with retromer dependent recycling.
Title: Phospholipase D activity couples plasma membrane endocytosis with retromer dependent recycling.

Propose that MIGA proteins promote mitochondrial fusion by regulating mitochondrial phospholipid metabolism via MitoPLD.
Title: Mitoguardin Regulates Mitochondrial Fusion through MitoPLD and Is Required for Neuronal Homeostasis.

The results suggest that Pld functions to promote trafficking of Golgi-derived fusion-competent vesicles during cellularization.
Title: A role for Phospholipase D in Drosophila embryonic cellularization.

The following Pld gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pld cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09021	NM_165469.4 Latest version!	Drosophila melanogaster phospholipase D, transcript variant A (Pld), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa09021	NM_078903.4 Latest version!	Drosophila melanogaster phospholipase D, transcript variant D (Pld), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa09021	NM_165470.3 Latest version!	Drosophila melanogaster phospholipase D, transcript variant B (Pld), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa09021	NM_165471.3 Latest version!	Drosophila melanogaster phospholipase D, transcript variant C (Pld), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa09023	NM_165472.3 Latest version!	Drosophila melanogaster phospholipase D, transcript variant G (Pld), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa09022	NM_001144138.2 Latest version!	Drosophila melanogaster phospholipase D, transcript variant F (Pld), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pld ( NM_165470.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724470.2 Drosophila melanogaster phospholipase D, transcript variant B (Pld), mRNA.

Price & Availability↑

CloneID	OFa09021
Clone ID Related Accession (Same CDS sequence)	NM_165470.3 , NM_078903.4 , NM_165469.4 , NM_165471.3
Accession Version	NM_165470.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phospholipase D, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165470.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGATG CCGGCACCGT TGGAGGCGCC TTGCCGCCCT ACGCACTTAC CAACTCGGAC 
GAAGAACATG GCAGCGGGGA AGAGGACGCG TCGGAGGAGA ACTCCAACAA TGAAGAGGGA 
GAGGGAGTGT TCCGGGACTG CACAGACGAA GCGGTTGTCG AGCATCATAA CCGCTGCCTG 
CCAGAATTTC AGTTCTCTCT AGTCGATTCT GAGTACGATG AGACCCTCGC TTTTCCTGAT 
TCTGTGACCA TTCTATCCAA CGTGGGCGAC AAGCCGGTGC TGGTGGAGCG CAAGGAGACG 
GACGATGATG AGGAGGAGTT CGACGACGAG GAAAACAACA GTGTAGTCCT GAGACACGAA 
ATACCATTTA CTAGCATATA CGGGCCGAGC GTCAAGTTCA ACTCGTTCCA GCGCAAGGTT 
TTCATCCCGG GCCGTGAGAT TCATGTTCGG ATCGTCGATA CGGAGCGTAG CGTCACTACA 
CATCTGCTAA ACCCCAATCT GTACACAATC GAGCTGACCC ACGGTCCCTT CAAGTGGACG 
ATCAAGCGGC GATACAAGCA CTTTAACTCG TTGCACCAGC AGCTCAGCTT TTTCCGCACC 
TCGCTCAACA TTCCTTTTCC CAGTCGCAGT CACAAGGAGA AGCGTACCAC TTTGAAAGCC 
ACAGCCAGAG AGATGGCTGA CGAGTCCACT CTAAAGGACC TTCCTTCTCA CACCAAGGTC 
AAACAAACTA GCACTCCGCT GAGGGCTGAA GGCAGAAGCA GTAAAATCGC GGGCAGTAAC 
GCCAACAATG CCATGGCTAT GATCAGTCCC AATCACAGCT CCATTCTGGC GGGTCTAACA 
CCACGACGCA TTCAAAAGAA GCGCAAAAAA AAGAAGAAAC GGAAGCTGCC GCGATTCCCA 
AACCGTCCTG AGAGTCTGGT CACCGTAGAG AATCTGAGCG TCAGAATAAA ACAGCTGGAG 
GACTACTTGT ACAACCTGCT GAACATCAGC TTGTACCGAT CTCACCATGA AACGCTAAAC 
TTCGTTGAAG TGTCTAATGT GTCCTTTGTT CCGGGAATGG GAATTAAGGG CAAGGAAGGC 
GTGATTTTAA AGCGAACTGG ATCAACGAGA CCAGGGCAAG CAGGATGCAA TTTTTTTGGG 
TGCTTTCAAA AGAACTGCTG TGTGCGCTGC AACTACTTTT GCTCCGACGT AGTTTGCGGC 
ACGTGGCGGA ACCGATGGTT TTTCGTAAAA GAGACCTGCT TCGGCTACAT CCGTCCAACA 
GACGGAAGCA TCCGGGCAGT GATCCTCTTT GATCAGGGCT TCGACGTTTC CACGGGTATC 
TATCAGACGG GCATGCGCAA GGGCTTGCAG GTACTGACGA ACAACCGTCA CATTGTGCTC 
AAGTGCTGGA CACGGCGTAA GTGTAAAGAG TGGATGCAAT ACCTCAAGAA CACGGCCAAC 
TCGTATGCGC GCGACTTCAC CCTGCCCAAT CCGCACATGT CCTTCGCTCC GATGCGCGCC 
AACACTCATG CCACGTGGTA TGTGGACGGC GCCCAGTATA TGTCAGCCGT GGCCGACGGC 
TTAGAGGCAG CCCTCGAGGA GATCTATATT GCCGACTGGT GGCTCAGTCC CGAGATATAC 
ATGAAGCGAC CCGCACTCGA CGGAGACTAC TGGCGATTGG ACAAGATCCT GTTGCGCAAG 
GCCGAACAGG GAGTGCGCGT CTTTGTGCTG CTCTACAAGG AGGTTGAAAT GGCACTTGGC 
ATAAACAGCT ACTACAGCAA GTCCACGCTG GCCAAGCATG AAAACATCAA GGTCATGCGT 
CATCCGGACC ATGCTAGAGG AGGTATTCTG CTTTGGGCAC ATCACGAAAA GATCGTCGTA 
ATCGACCAAA CCTATGCGTT TATGGGAGGT ATTGATTTGT GCTATGGACG TTGGGATGAT 
CACCACCATC GGCTAACGGA TCTGGGTAGC ATATCTACGT CATCTTTTTC TGGCAGCACG 
CGTCGAACGC CAAGTTTGTA CTTCACCAAA GACGACACGG ACTCAGCTTT CGGATCACGT 
AAGTCCTCGC GAAACGCTCA CTACGATACC TCCGCCAAGG AAAGGCCACC GTCCCCACCC 
CCGGATGAGC CCAATACTAG CATAGAGTTG AAAACTCTTA AGCCTGGTGA TCGACTGCTT 
ATACCGTCTA CGCTCGTTTC GAGTCCGGGT GAAACTCCCG CAGAATCGGG AATCGCTTTA 
GAAGGGATGA AACTCAACAC CCCTGAAATG GAGCGTAAGA ACGTACTCGA TCGCCTGAAG 
AACAACGCGA TGAAGGGCGC CCGTATGGGC AAGGACTTTA TGCACCGTCT AACAGCTACT 
GAGACGGAGG AAAAATCTGC GGAGGTGTAC ACTATCGAGT CCGAGGAAGC TACGGACCAC 
GAAGTCAACC TTAACATGGC TTCAGGTGGG CAGGAAGTGG CGATTACCAC TAGCAGTACA 
CAAATACTCA GTGAGTTCTG CGGCCAGGCC AAGTACTGGT TCGGCAAGGA TTACTCCAAC 
TTTATACTTA AAGACTGGAT GAACCTAAAC TCGCCGTTCG TGGATATCAT AGATCGAACA 
ACAACACCGC GGATGCCATG GCACGACGTG GGTCTGTGTG TGGTGGGTAC TTCCGCTAGG 
GATGTGGCCC GCCACTTCAT TCAGCGCTGG AATGCCATGA AGCTGGAGAA ACTACGCGAT 
AACACGAGAT TCCCCTATTT GATGCCAAAA AGCTATCACC AAGTGAGGCT CAATCCGAAC 
ATTCAGCAAA ACCGTCAGCA ACGGGTCACG TGCCAGCTAC TTCGAAGCGT CTCTGCCTGG 
AGCTGCGGCT TTATAGAGGC GGATCTTGTG GAGCAAAGCA TCCACGATGC CTACATCCAG 
ACGATCACCA AGGCGCAGCA CTACGTGTAC ATCGAAAACC AATTTTTTAT CACTATGCAG 
TTAGGCATGG GTGTGCCAGG TGCTTATAAC AATGTGCGGA ATCAAATCGG GGAAACACTC 
TTTAAACGGA TCGTTAGAGC GCACAAGGAA CGGAAGCCTT TCCGAGTTTA TGTGATTATG 
CCGCTCCTAC CGGGCTTTGA GGGTGATGTC GGTGGCAGTA CTGGGATAGC AGTCAGAGCA 
ATTACACACT GGAACTATGC GTCCATTTCC AGGGGACGCA CATCAATTTT GACCCGCCTG 
CAGGAGGCGG GTATTGCCAA TCCGGAAAAC TATATCTCAT TCCACAGCCT GCGCAACCAT 
TCTTTTTTGA ATAACACACC CATAACAGAG TTGATATATG TCCACTCAAA GCTCTTGATA 
GCCGACGATC GCGTTGTAAT CTGCGGTTCG GCAAACATTA ACGATCGCTC TATGATCGGA 
AAGCGGGACT CCGAGATAGC GGCTATTCTA ATGGACGAGG AGTTCGAGGA CGGACGCATG 
AATGGCAAGA AGTATCCGAG CGGAGTGTTT GCCGGTCGCC TTCGAAAATA CCTTTTTAAA 
GAACACTTAG GCCTCCTGGA AAGCGAAGGT TCCAGTCGGT CTGACCTGGA CATTAACGAT 
CCTGTTTGTG AGAAGTTTTG GCACGGCACC TGGCGTAGGA TTTCAATGCA GAACACAGAG 
ATTTACGACG AGGTGTTTAA GTGCATCCCC ACTGACTTTG TAAAAACCTT TGCCAGCCTT 
CGCAAATACC AGGAGGAGCC GCCTCTTGCC AAAACCGCCC CTGATCTAGC TGCCAACAGA 
GCCAACGACA TTCAGGGTTA CTTGGTCGAC CTGCCATTGG AATTTCTGAA CAAGGAGGTT 
CTCACGCCGC CTGGAACTAG TAAGGAGGGC CTAATCCCTA CCTCTGTATG GACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724470.2
CDS	824..4660
Translation	MADAGTVGGALPPYALTNSDEEHGSGEEDASEENSNNEEGEGVFRDCTDEAVVEHHNRCLPEFQFSLVDSEYDETLAFPDSVTILSNVGDKPVLVERKETDDDEEEFDDEENNSVVLRHEIPFTSIYGPSVKFNSFQRKVFIPGREIHVRIVDTERSVTTHLLNPNLYTIELTHGPFKWTIKRRYKHFNSLHQQLSFFRTSLNIPFPSRSHKEKRTTLKATAREMADESTLKDLPSHTKVKQTSTPLRAEGRSSKIAGSNANNAMAMISPNHSSILAGLTPRRIQKKRKKKKKRKLPRFPNRPESLVTVENLSVRIKQLEDYLYNLLNISLYRSHHETLNFVEVSNVSFVPGMGIKGKEGVILKRTGSTRPGQAGCNFFGCFQKNCCVRCNYFCSDVVCGTWRNRWFFVKETCFGYIRPTDGSIRAVILFDQGFDVSTGIYQTGMRKGLQVLTNNRHIVLKCWTRRKCKEWMQYLKNTANSYARDFTLPNPHMSFAPMRANTHATWYVDGAQYMSAVADGLEAALEEIYIADWWLSPEIYMKRPALDGDYWRLDKILLRKAEQGVRVFVLLYKEVEMALGINSYYSKSTLAKHENIKVMRHPDHARGGILLWAHHEKIVVIDQTYAFMGGIDLCYGRWDDHHHRLTDLGSISTSSFSGSTRRTPSLYFTKDDTDSAFGSRKSSRNAHYDTSAKERPPSPPPDEPNTSIELKTLKPGDRLLIPSTLVSSPGETPAESGIALEGMKLNTPEMERKNVLDRLKNNAMKGARMGKDFMHRLTATETEEKSAEVYTIESEEATDHEVNLNMASGGQEVAITTSSTQILSEFCGQAKYWFGKDYSNFILKDWMNLNSPFVDIIDRTTTPRMPWHDVGLCVVGTSARDVARHFIQRWNAMKLEKLRDNTRFPYLMPKSYHQVRLNPNIQQNRQQRVTCQLLRSVSAWSCGFIEADLVEQSIHDAYIQTITKAQHYVYIENQFFITMQLGMGVPGAYNNVRNQIGETLFKRIVRAHKERKPFRVYVIMPLLPGFEGDVGGSTGIAVRAITHWNYASISRGRTSILTRLQEAGIANPENYISFHSLRNHSFLNNTPITELIYVHSKLLIADDRVVICGSANINDRSMIGKRDSEIAAILMDEEFEDGRMNGKKYPSGVFAGRLRKYLFKEHLGLLESEGSSRSDLDINDPVCEKFWHGTWRRISMQNTEIYDEVFKCIPTDFVKTFASLRKYQEEPPLAKTAPDLAANRANDIQGYLVDLPLEFLNKEVLTPPGTSKEGLIPTSVWT

Target ORF information:

RefSeq Version	NM_165470.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phospholipase D, transcript variant B (Pld), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165470.3

ORF Insert Sequence:

ATGGCAGATG CCGGCACCGT TGGAGGCGCC TTGCCGCCCT ACGCACTTAC CAACTCGGAC 
GAAGAACATG GCAGCGGGGA AGAGGACGCG TCGGAGGAGA ACTCCAACAA TGAAGAGGGA 
GAGGGAGTGT TCCGGGACTG CACAGACGAA GCGGTTGTCG AGCATCATAA CCGCTGCCTG 
CCAGAATTTC AGTTCTCTCT AGTCGATTCT GAGTACGATG AGACCCTCGC TTTTCCTGAT 
TCTGTGACCA TTCTATCCAA CGTGGGCGAC AAGCCGGTGC TGGTGGAGCG CAAGGAGACG 
GACGATGATG AGGAGGAGTT CGACGACGAG GAAAACAACA GTGTAGTCCT GAGACACGAA 
ATACCATTTA CTAGCATATA CGGGCCGAGC GTCAAGTTCA ACTCGTTCCA GCGCAAGGTT 
TTCATCCCGG GCCGTGAGAT TCATGTTCGG ATCGTCGATA CGGAGCGTAG CGTCACTACA 
CATCTGCTAA ACCCCAATCT GTACACAATC GAGCTGACCC ACGGTCCCTT CAAGTGGACG 
ATCAAGCGGC GATACAAGCA CTTTAACTCG TTGCACCAGC AGCTCAGCTT TTTCCGCACC 
TCGCTCAACA TTCCTTTTCC CAGTCGCAGT CACAAGGAGA AGCGTACCAC TTTGAAAGCC 
ACAGCCAGAG AGATGGCTGA CGAGTCCACT CTAAAGGACC TTCCTTCTCA CACCAAGGTC 
AAACAAACTA GCACTCCGCT GAGGGCTGAA GGCAGAAGCA GTAAAATCGC GGGCAGTAAC 
GCCAACAATG CCATGGCTAT GATCAGTCCC AATCACAGCT CCATTCTGGC GGGTCTAACA 
CCACGACGCA TTCAAAAGAA GCGCAAAAAA AAGAAGAAAC GGAAGCTGCC GCGATTCCCA 
AACCGTCCTG AGAGTCTGGT CACCGTAGAG AATCTGAGCG TCAGAATAAA ACAGCTGGAG 
GACTACTTGT ACAACCTGCT GAACATCAGC TTGTACCGAT CTCACCATGA AACGCTAAAC 
TTCGTTGAAG TGTCTAATGT GTCCTTTGTT CCGGGAATGG GAATTAAGGG CAAGGAAGGC 
GTGATTTTAA AGCGAACTGG ATCAACGAGA CCAGGGCAAG CAGGATGCAA TTTTTTTGGG 
TGCTTTCAAA AGAACTGCTG TGTGCGCTGC AACTACTTTT GCTCCGACGT AGTTTGCGGC 
ACGTGGCGGA ACCGATGGTT TTTCGTAAAA GAGACCTGCT TCGGCTACAT CCGTCCAACA 
GACGGAAGCA TCCGGGCAGT GATCCTCTTT GATCAGGGCT TCGACGTTTC CACGGGTATC 
TATCAGACGG GCATGCGCAA GGGCTTGCAG GTACTGACGA ACAACCGTCA CATTGTGCTC 
AAGTGCTGGA CACGGCGTAA GTGTAAAGAG TGGATGCAAT ACCTCAAGAA CACGGCCAAC 
TCGTATGCGC GCGACTTCAC CCTGCCCAAT CCGCACATGT CCTTCGCTCC GATGCGCGCC 
AACACTCATG CCACGTGGTA TGTGGACGGC GCCCAGTATA TGTCAGCCGT GGCCGACGGC 
TTAGAGGCAG CCCTCGAGGA GATCTATATT GCCGACTGGT GGCTCAGTCC CGAGATATAC 
ATGAAGCGAC CCGCACTCGA CGGAGACTAC TGGCGATTGG ACAAGATCCT GTTGCGCAAG 
GCCGAACAGG GAGTGCGCGT CTTTGTGCTG CTCTACAAGG AGGTTGAAAT GGCACTTGGC 
ATAAACAGCT ACTACAGCAA GTCCACGCTG GCCAAGCATG AAAACATCAA GGTCATGCGT 
CATCCGGACC ATGCTAGAGG AGGTATTCTG CTTTGGGCAC ATCACGAAAA GATCGTCGTA 
ATCGACCAAA CCTATGCGTT TATGGGAGGT ATTGATTTGT GCTATGGACG TTGGGATGAT 
CACCACCATC GGCTAACGGA TCTGGGTAGC ATATCTACGT CATCTTTTTC TGGCAGCACG 
CGTCGAACGC CAAGTTTGTA CTTCACCAAA GACGACACGG ACTCAGCTTT CGGATCACGT 
AAGTCCTCGC GAAACGCTCA CTACGATACC TCCGCCAAGG AAAGGCCACC GTCCCCACCC 
CCGGATGAGC CCAATACTAG CATAGAGTTG AAAACTCTTA AGCCTGGTGA TCGACTGCTT 
ATACCGTCTA CGCTCGTTTC GAGTCCGGGT GAAACTCCCG CAGAATCGGG AATCGCTTTA 
GAAGGGATGA AACTCAACAC CCCTGAAATG GAGCGTAAGA ACGTACTCGA TCGCCTGAAG 
AACAACGCGA TGAAGGGCGC CCGTATGGGC AAGGACTTTA TGCACCGTCT AACAGCTACT 
GAGACGGAGG AAAAATCTGC GGAGGTGTAC ACTATCGAGT CCGAGGAAGC TACGGACCAC 
GAAGTCAACC TTAACATGGC TTCAGGTGGG CAGGAAGTGG CGATTACCAC TAGCAGTACA 
CAAATACTCA GTGAGTTCTG CGGCCAGGCC AAGTACTGGT TCGGCAAGGA TTACTCCAAC 
TTTATACTTA AAGACTGGAT GAACCTAAAC TCGCCGTTCG TGGATATCAT AGATCGAACA 
ACAACACCGC GGATGCCATG GCACGACGTG GGTCTGTGTG TGGTGGGTAC TTCCGCTAGG 
GATGTGGCCC GCCACTTCAT TCAGCGCTGG AATGCCATGA AGCTGGAGAA ACTACGCGAT 
AACACGAGAT TCCCCTATTT GATGCCAAAA AGCTATCACC AAGTGAGGCT CAATCCGAAC 
ATTCAGCAAA ACCGTCAGCA ACGGGTCACG TGCCAGCTAC TTCGAAGCGT CTCTGCCTGG 
AGCTGCGGCT TTATAGAGGC GGATCTTGTG GAGCAAAGCA TCCACGATGC CTACATCCAG 
ACGATCACCA AGGCGCAGCA CTACGTGTAC ATCGAAAACC AATTTTTTAT CACTATGCAG 
TTAGGCATGG GTGTGCCAGG TGCTTATAAC AATGTGCGGA ATCAAATCGG GGAAACACTC 
TTTAAACGGA TCGTTAGAGC GCACAAGGAA CGGAAGCCTT TCCGAGTTTA TGTGATTATG 
CCGCTCCTAC CGGGCTTTGA GGGTGATGTC GGTGGCAGTA CTGGGATAGC AGTCAGAGCA 
ATTACACACT GGAACTATGC GTCCATTTCC AGGGGACGCA CATCAATTTT GACCCGCCTG 
CAGGAGGCGG GTATTGCCAA TCCGGAAAAC TATATCTCAT TCCACAGCCT GCGCAACCAT 
TCTTTTTTGA ATAACACACC CATAACAGAG TTGATATATG TCCACTCAAA GCTCTTGATA 
GCCGACGATC GCGTTGTAAT CTGCGGTTCG GCAAACATTA ACGATCGCTC TATGATCGGA 
AAGCGGGACT CCGAGATAGC GGCTATTCTA ATGGACGAGG AGTTCGAGGA CGGACGCATG 
AATGGCAAGA AGTATCCGAG CGGAGTGTTT GCCGGTCGCC TTCGAAAATA CCTTTTTAAA 
GAACACTTAG GCCTCCTGGA AAGCGAAGGT TCCAGTCGGT CTGACCTGGA CATTAACGAT 
CCTGTTTGTG AGAAGTTTTG GCACGGCACC TGGCGTAGGA TTTCAATGCA GAACACAGAG 
ATTTACGACG AGGTGTTTAA GTGCATCCCC ACTGACTTTG TAAAAACCTT TGCCAGCCTT 
CGCAAATACC AGGAGGAGCC GCCTCTTGCC AAAACCGCCC CTGATCTAGC TGCCAACAGA 
GCCAACGACA TTCAGGGTTA CTTGGTCGAC CTGCCATTGG AATTTCTGAA CAAGGAGGTT 
CTCACGCCGC CTGGAACTAG TAAGGAGGGC CTAATCCCTA CCTCTGTATG GACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pld ( NM_078903.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523627.3 Drosophila melanogaster phospholipase D, transcript variant D (Pld), mRNA.

Price & Availability↑

CloneID	OFa09021
Clone ID Related Accession (Same CDS sequence)	NM_165470.3 , NM_078903.4 , NM_165469.4 , NM_165471.3
Accession Version	NM_078903.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phospholipase D, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_078903.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGATG CCGGCACCGT TGGAGGCGCC TTGCCGCCCT ACGCACTTAC CAACTCGGAC 
GAAGAACATG GCAGCGGGGA AGAGGACGCG TCGGAGGAGA ACTCCAACAA TGAAGAGGGA 
GAGGGAGTGT TCCGGGACTG CACAGACGAA GCGGTTGTCG AGCATCATAA CCGCTGCCTG 
CCAGAATTTC AGTTCTCTCT AGTCGATTCT GAGTACGATG AGACCCTCGC TTTTCCTGAT 
TCTGTGACCA TTCTATCCAA CGTGGGCGAC AAGCCGGTGC TGGTGGAGCG CAAGGAGACG 
GACGATGATG AGGAGGAGTT CGACGACGAG GAAAACAACA GTGTAGTCCT GAGACACGAA 
ATACCATTTA CTAGCATATA CGGGCCGAGC GTCAAGTTCA ACTCGTTCCA GCGCAAGGTT 
TTCATCCCGG GCCGTGAGAT TCATGTTCGG ATCGTCGATA CGGAGCGTAG CGTCACTACA 
CATCTGCTAA ACCCCAATCT GTACACAATC GAGCTGACCC ACGGTCCCTT CAAGTGGACG 
ATCAAGCGGC GATACAAGCA CTTTAACTCG TTGCACCAGC AGCTCAGCTT TTTCCGCACC 
TCGCTCAACA TTCCTTTTCC CAGTCGCAGT CACAAGGAGA AGCGTACCAC TTTGAAAGCC 
ACAGCCAGAG AGATGGCTGA CGAGTCCACT CTAAAGGACC TTCCTTCTCA CACCAAGGTC 
AAACAAACTA GCACTCCGCT GAGGGCTGAA GGCAGAAGCA GTAAAATCGC GGGCAGTAAC 
GCCAACAATG CCATGGCTAT GATCAGTCCC AATCACAGCT CCATTCTGGC GGGTCTAACA 
CCACGACGCA TTCAAAAGAA GCGCAAAAAA AAGAAGAAAC GGAAGCTGCC GCGATTCCCA 
AACCGTCCTG AGAGTCTGGT CACCGTAGAG AATCTGAGCG TCAGAATAAA ACAGCTGGAG 
GACTACTTGT ACAACCTGCT GAACATCAGC TTGTACCGAT CTCACCATGA AACGCTAAAC 
TTCGTTGAAG TGTCTAATGT GTCCTTTGTT CCGGGAATGG GAATTAAGGG CAAGGAAGGC 
GTGATTTTAA AGCGAACTGG ATCAACGAGA CCAGGGCAAG CAGGATGCAA TTTTTTTGGG 
TGCTTTCAAA AGAACTGCTG TGTGCGCTGC AACTACTTTT GCTCCGACGT AGTTTGCGGC 
ACGTGGCGGA ACCGATGGTT TTTCGTAAAA GAGACCTGCT TCGGCTACAT CCGTCCAACA 
GACGGAAGCA TCCGGGCAGT GATCCTCTTT GATCAGGGCT TCGACGTTTC CACGGGTATC 
TATCAGACGG GCATGCGCAA GGGCTTGCAG GTACTGACGA ACAACCGTCA CATTGTGCTC 
AAGTGCTGGA CACGGCGTAA GTGTAAAGAG TGGATGCAAT ACCTCAAGAA CACGGCCAAC 
TCGTATGCGC GCGACTTCAC CCTGCCCAAT CCGCACATGT CCTTCGCTCC GATGCGCGCC 
AACACTCATG CCACGTGGTA TGTGGACGGC GCCCAGTATA TGTCAGCCGT GGCCGACGGC 
TTAGAGGCAG CCCTCGAGGA GATCTATATT GCCGACTGGT GGCTCAGTCC CGAGATATAC 
ATGAAGCGAC CCGCACTCGA CGGAGACTAC TGGCGATTGG ACAAGATCCT GTTGCGCAAG 
GCCGAACAGG GAGTGCGCGT CTTTGTGCTG CTCTACAAGG AGGTTGAAAT GGCACTTGGC 
ATAAACAGCT ACTACAGCAA GTCCACGCTG GCCAAGCATG AAAACATCAA GGTCATGCGT 
CATCCGGACC ATGCTAGAGG AGGTATTCTG CTTTGGGCAC ATCACGAAAA GATCGTCGTA 
ATCGACCAAA CCTATGCGTT TATGGGAGGT ATTGATTTGT GCTATGGACG TTGGGATGAT 
CACCACCATC GGCTAACGGA TCTGGGTAGC ATATCTACGT CATCTTTTTC TGGCAGCACG 
CGTCGAACGC CAAGTTTGTA CTTCACCAAA GACGACACGG ACTCAGCTTT CGGATCACGT 
AAGTCCTCGC GAAACGCTCA CTACGATACC TCCGCCAAGG AAAGGCCACC GTCCCCACCC 
CCGGATGAGC CCAATACTAG CATAGAGTTG AAAACTCTTA AGCCTGGTGA TCGACTGCTT 
ATACCGTCTA CGCTCGTTTC GAGTCCGGGT GAAACTCCCG CAGAATCGGG AATCGCTTTA 
GAAGGGATGA AACTCAACAC CCCTGAAATG GAGCGTAAGA ACGTACTCGA TCGCCTGAAG 
AACAACGCGA TGAAGGGCGC CCGTATGGGC AAGGACTTTA TGCACCGTCT AACAGCTACT 
GAGACGGAGG AAAAATCTGC GGAGGTGTAC ACTATCGAGT CCGAGGAAGC TACGGACCAC 
GAAGTCAACC TTAACATGGC TTCAGGTGGG CAGGAAGTGG CGATTACCAC TAGCAGTACA 
CAAATACTCA GTGAGTTCTG CGGCCAGGCC AAGTACTGGT TCGGCAAGGA TTACTCCAAC 
TTTATACTTA AAGACTGGAT GAACCTAAAC TCGCCGTTCG TGGATATCAT AGATCGAACA 
ACAACACCGC GGATGCCATG GCACGACGTG GGTCTGTGTG TGGTGGGTAC TTCCGCTAGG 
GATGTGGCCC GCCACTTCAT TCAGCGCTGG AATGCCATGA AGCTGGAGAA ACTACGCGAT 
AACACGAGAT TCCCCTATTT GATGCCAAAA AGCTATCACC AAGTGAGGCT CAATCCGAAC 
ATTCAGCAAA ACCGTCAGCA ACGGGTCACG TGCCAGCTAC TTCGAAGCGT CTCTGCCTGG 
AGCTGCGGCT TTATAGAGGC GGATCTTGTG GAGCAAAGCA TCCACGATGC CTACATCCAG 
ACGATCACCA AGGCGCAGCA CTACGTGTAC ATCGAAAACC AATTTTTTAT CACTATGCAG 
TTAGGCATGG GTGTGCCAGG TGCTTATAAC AATGTGCGGA ATCAAATCGG GGAAACACTC 
TTTAAACGGA TCGTTAGAGC GCACAAGGAA CGGAAGCCTT TCCGAGTTTA TGTGATTATG 
CCGCTCCTAC CGGGCTTTGA GGGTGATGTC GGTGGCAGTA CTGGGATAGC AGTCAGAGCA 
ATTACACACT GGAACTATGC GTCCATTTCC AGGGGACGCA CATCAATTTT GACCCGCCTG 
CAGGAGGCGG GTATTGCCAA TCCGGAAAAC TATATCTCAT TCCACAGCCT GCGCAACCAT 
TCTTTTTTGA ATAACACACC CATAACAGAG TTGATATATG TCCACTCAAA GCTCTTGATA 
GCCGACGATC GCGTTGTAAT CTGCGGTTCG GCAAACATTA ACGATCGCTC TATGATCGGA 
AAGCGGGACT CCGAGATAGC GGCTATTCTA ATGGACGAGG AGTTCGAGGA CGGACGCATG 
AATGGCAAGA AGTATCCGAG CGGAGTGTTT GCCGGTCGCC TTCGAAAATA CCTTTTTAAA 
GAACACTTAG GCCTCCTGGA AAGCGAAGGT TCCAGTCGGT CTGACCTGGA CATTAACGAT 
CCTGTTTGTG AGAAGTTTTG GCACGGCACC TGGCGTAGGA TTTCAATGCA GAACACAGAG 
ATTTACGACG AGGTGTTTAA GTGCATCCCC ACTGACTTTG TAAAAACCTT TGCCAGCCTT 
CGCAAATACC AGGAGGAGCC GCCTCTTGCC AAAACCGCCC CTGATCTAGC TGCCAACAGA 
GCCAACGACA TTCAGGGTTA CTTGGTCGAC CTGCCATTGG AATTTCTGAA CAAGGAGGTT 
CTCACGCCGC CTGGAACTAG TAAGGAGGGC CTAATCCCTA CCTCTGTATG GACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523627.3
CDS	174..4010
Translation	MADAGTVGGALPPYALTNSDEEHGSGEEDASEENSNNEEGEGVFRDCTDEAVVEHHNRCLPEFQFSLVDSEYDETLAFPDSVTILSNVGDKPVLVERKETDDDEEEFDDEENNSVVLRHEIPFTSIYGPSVKFNSFQRKVFIPGREIHVRIVDTERSVTTHLLNPNLYTIELTHGPFKWTIKRRYKHFNSLHQQLSFFRTSLNIPFPSRSHKEKRTTLKATAREMADESTLKDLPSHTKVKQTSTPLRAEGRSSKIAGSNANNAMAMISPNHSSILAGLTPRRIQKKRKKKKKRKLPRFPNRPESLVTVENLSVRIKQLEDYLYNLLNISLYRSHHETLNFVEVSNVSFVPGMGIKGKEGVILKRTGSTRPGQAGCNFFGCFQKNCCVRCNYFCSDVVCGTWRNRWFFVKETCFGYIRPTDGSIRAVILFDQGFDVSTGIYQTGMRKGLQVLTNNRHIVLKCWTRRKCKEWMQYLKNTANSYARDFTLPNPHMSFAPMRANTHATWYVDGAQYMSAVADGLEAALEEIYIADWWLSPEIYMKRPALDGDYWRLDKILLRKAEQGVRVFVLLYKEVEMALGINSYYSKSTLAKHENIKVMRHPDHARGGILLWAHHEKIVVIDQTYAFMGGIDLCYGRWDDHHHRLTDLGSISTSSFSGSTRRTPSLYFTKDDTDSAFGSRKSSRNAHYDTSAKERPPSPPPDEPNTSIELKTLKPGDRLLIPSTLVSSPGETPAESGIALEGMKLNTPEMERKNVLDRLKNNAMKGARMGKDFMHRLTATETEEKSAEVYTIESEEATDHEVNLNMASGGQEVAITTSSTQILSEFCGQAKYWFGKDYSNFILKDWMNLNSPFVDIIDRTTTPRMPWHDVGLCVVGTSARDVARHFIQRWNAMKLEKLRDNTRFPYLMPKSYHQVRLNPNIQQNRQQRVTCQLLRSVSAWSCGFIEADLVEQSIHDAYIQTITKAQHYVYIENQFFITMQLGMGVPGAYNNVRNQIGETLFKRIVRAHKERKPFRVYVIMPLLPGFEGDVGGSTGIAVRAITHWNYASISRGRTSILTRLQEAGIANPENYISFHSLRNHSFLNNTPITELIYVHSKLLIADDRVVICGSANINDRSMIGKRDSEIAAILMDEEFEDGRMNGKKYPSGVFAGRLRKYLFKEHLGLLESEGSSRSDLDINDPVCEKFWHGTWRRISMQNTEIYDEVFKCIPTDFVKTFASLRKYQEEPPLAKTAPDLAANRANDIQGYLVDLPLEFLNKEVLTPPGTSKEGLIPTSVWT

Target ORF information:

RefSeq Version	NM_078903.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phospholipase D, transcript variant D (Pld), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078903.4

ORF Insert Sequence:

ATGGCAGATG CCGGCACCGT TGGAGGCGCC TTGCCGCCCT ACGCACTTAC CAACTCGGAC 
GAAGAACATG GCAGCGGGGA AGAGGACGCG TCGGAGGAGA ACTCCAACAA TGAAGAGGGA 
GAGGGAGTGT TCCGGGACTG CACAGACGAA GCGGTTGTCG AGCATCATAA CCGCTGCCTG 
CCAGAATTTC AGTTCTCTCT AGTCGATTCT GAGTACGATG AGACCCTCGC TTTTCCTGAT 
TCTGTGACCA TTCTATCCAA CGTGGGCGAC AAGCCGGTGC TGGTGGAGCG CAAGGAGACG 
GACGATGATG AGGAGGAGTT CGACGACGAG GAAAACAACA GTGTAGTCCT GAGACACGAA 
ATACCATTTA CTAGCATATA CGGGCCGAGC GTCAAGTTCA ACTCGTTCCA GCGCAAGGTT 
TTCATCCCGG GCCGTGAGAT TCATGTTCGG ATCGTCGATA CGGAGCGTAG CGTCACTACA 
CATCTGCTAA ACCCCAATCT GTACACAATC GAGCTGACCC ACGGTCCCTT CAAGTGGACG 
ATCAAGCGGC GATACAAGCA CTTTAACTCG TTGCACCAGC AGCTCAGCTT TTTCCGCACC 
TCGCTCAACA TTCCTTTTCC CAGTCGCAGT CACAAGGAGA AGCGTACCAC TTTGAAAGCC 
ACAGCCAGAG AGATGGCTGA CGAGTCCACT CTAAAGGACC TTCCTTCTCA CACCAAGGTC 
AAACAAACTA GCACTCCGCT GAGGGCTGAA GGCAGAAGCA GTAAAATCGC GGGCAGTAAC 
GCCAACAATG CCATGGCTAT GATCAGTCCC AATCACAGCT CCATTCTGGC GGGTCTAACA 
CCACGACGCA TTCAAAAGAA GCGCAAAAAA AAGAAGAAAC GGAAGCTGCC GCGATTCCCA 
AACCGTCCTG AGAGTCTGGT CACCGTAGAG AATCTGAGCG TCAGAATAAA ACAGCTGGAG 
GACTACTTGT ACAACCTGCT GAACATCAGC TTGTACCGAT CTCACCATGA AACGCTAAAC 
TTCGTTGAAG TGTCTAATGT GTCCTTTGTT CCGGGAATGG GAATTAAGGG CAAGGAAGGC 
GTGATTTTAA AGCGAACTGG ATCAACGAGA CCAGGGCAAG CAGGATGCAA TTTTTTTGGG 
TGCTTTCAAA AGAACTGCTG TGTGCGCTGC AACTACTTTT GCTCCGACGT AGTTTGCGGC 
ACGTGGCGGA ACCGATGGTT TTTCGTAAAA GAGACCTGCT TCGGCTACAT CCGTCCAACA 
GACGGAAGCA TCCGGGCAGT GATCCTCTTT GATCAGGGCT TCGACGTTTC CACGGGTATC 
TATCAGACGG GCATGCGCAA GGGCTTGCAG GTACTGACGA ACAACCGTCA CATTGTGCTC 
AAGTGCTGGA CACGGCGTAA GTGTAAAGAG TGGATGCAAT ACCTCAAGAA CACGGCCAAC 
TCGTATGCGC GCGACTTCAC CCTGCCCAAT CCGCACATGT CCTTCGCTCC GATGCGCGCC 
AACACTCATG CCACGTGGTA TGTGGACGGC GCCCAGTATA TGTCAGCCGT GGCCGACGGC 
TTAGAGGCAG CCCTCGAGGA GATCTATATT GCCGACTGGT GGCTCAGTCC CGAGATATAC 
ATGAAGCGAC CCGCACTCGA CGGAGACTAC TGGCGATTGG ACAAGATCCT GTTGCGCAAG 
GCCGAACAGG GAGTGCGCGT CTTTGTGCTG CTCTACAAGG AGGTTGAAAT GGCACTTGGC 
ATAAACAGCT ACTACAGCAA GTCCACGCTG GCCAAGCATG AAAACATCAA GGTCATGCGT 
CATCCGGACC ATGCTAGAGG AGGTATTCTG CTTTGGGCAC ATCACGAAAA GATCGTCGTA 
ATCGACCAAA CCTATGCGTT TATGGGAGGT ATTGATTTGT GCTATGGACG TTGGGATGAT 
CACCACCATC GGCTAACGGA TCTGGGTAGC ATATCTACGT CATCTTTTTC TGGCAGCACG 
CGTCGAACGC CAAGTTTGTA CTTCACCAAA GACGACACGG ACTCAGCTTT CGGATCACGT 
AAGTCCTCGC GAAACGCTCA CTACGATACC TCCGCCAAGG AAAGGCCACC GTCCCCACCC 
CCGGATGAGC CCAATACTAG CATAGAGTTG AAAACTCTTA AGCCTGGTGA TCGACTGCTT 
ATACCGTCTA CGCTCGTTTC GAGTCCGGGT GAAACTCCCG CAGAATCGGG AATCGCTTTA 
GAAGGGATGA AACTCAACAC CCCTGAAATG GAGCGTAAGA ACGTACTCGA TCGCCTGAAG 
AACAACGCGA TGAAGGGCGC CCGTATGGGC AAGGACTTTA TGCACCGTCT AACAGCTACT 
GAGACGGAGG AAAAATCTGC GGAGGTGTAC ACTATCGAGT CCGAGGAAGC TACGGACCAC 
GAAGTCAACC TTAACATGGC TTCAGGTGGG CAGGAAGTGG CGATTACCAC TAGCAGTACA 
CAAATACTCA GTGAGTTCTG CGGCCAGGCC AAGTACTGGT TCGGCAAGGA TTACTCCAAC 
TTTATACTTA AAGACTGGAT GAACCTAAAC TCGCCGTTCG TGGATATCAT AGATCGAACA 
ACAACACCGC GGATGCCATG GCACGACGTG GGTCTGTGTG TGGTGGGTAC TTCCGCTAGG 
GATGTGGCCC GCCACTTCAT TCAGCGCTGG AATGCCATGA AGCTGGAGAA ACTACGCGAT 
AACACGAGAT TCCCCTATTT GATGCCAAAA AGCTATCACC AAGTGAGGCT CAATCCGAAC 
ATTCAGCAAA ACCGTCAGCA ACGGGTCACG TGCCAGCTAC TTCGAAGCGT CTCTGCCTGG 
AGCTGCGGCT TTATAGAGGC GGATCTTGTG GAGCAAAGCA TCCACGATGC CTACATCCAG 
ACGATCACCA AGGCGCAGCA CTACGTGTAC ATCGAAAACC AATTTTTTAT CACTATGCAG 
TTAGGCATGG GTGTGCCAGG TGCTTATAAC AATGTGCGGA ATCAAATCGG GGAAACACTC 
TTTAAACGGA TCGTTAGAGC GCACAAGGAA CGGAAGCCTT TCCGAGTTTA TGTGATTATG 
CCGCTCCTAC CGGGCTTTGA GGGTGATGTC GGTGGCAGTA CTGGGATAGC AGTCAGAGCA 
ATTACACACT GGAACTATGC GTCCATTTCC AGGGGACGCA CATCAATTTT GACCCGCCTG 
CAGGAGGCGG GTATTGCCAA TCCGGAAAAC TATATCTCAT TCCACAGCCT GCGCAACCAT 
TCTTTTTTGA ATAACACACC CATAACAGAG TTGATATATG TCCACTCAAA GCTCTTGATA 
GCCGACGATC GCGTTGTAAT CTGCGGTTCG GCAAACATTA ACGATCGCTC TATGATCGGA 
AAGCGGGACT CCGAGATAGC GGCTATTCTA ATGGACGAGG AGTTCGAGGA CGGACGCATG 
AATGGCAAGA AGTATCCGAG CGGAGTGTTT GCCGGTCGCC TTCGAAAATA CCTTTTTAAA 
GAACACTTAG GCCTCCTGGA AAGCGAAGGT TCCAGTCGGT CTGACCTGGA CATTAACGAT 
CCTGTTTGTG AGAAGTTTTG GCACGGCACC TGGCGTAGGA TTTCAATGCA GAACACAGAG 
ATTTACGACG AGGTGTTTAA GTGCATCCCC ACTGACTTTG TAAAAACCTT TGCCAGCCTT 
CGCAAATACC AGGAGGAGCC GCCTCTTGCC AAAACCGCCC CTGATCTAGC TGCCAACAGA 
GCCAACGACA TTCAGGGTTA CTTGGTCGAC CTGCCATTGG AATTTCTGAA CAAGGAGGTT 
CTCACGCCGC CTGGAACTAG TAAGGAGGGC CTAATCCCTA CCTCTGTATG GACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pld ( NM_165469.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724469.2 Drosophila melanogaster phospholipase D, transcript variant A (Pld), mRNA.

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CloneID	OFa09021
Clone ID Related Accession (Same CDS sequence)	NM_165470.3 , NM_078903.4 , NM_165469.4 , NM_165471.3
Accession Version	NM_165469.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phospholipase D, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165469.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGATG CCGGCACCGT TGGAGGCGCC TTGCCGCCCT ACGCACTTAC CAACTCGGAC 
GAAGAACATG GCAGCGGGGA AGAGGACGCG TCGGAGGAGA ACTCCAACAA TGAAGAGGGA 
GAGGGAGTGT TCCGGGACTG CACAGACGAA GCGGTTGTCG AGCATCATAA CCGCTGCCTG 
CCAGAATTTC AGTTCTCTCT AGTCGATTCT GAGTACGATG AGACCCTCGC TTTTCCTGAT 
TCTGTGACCA TTCTATCCAA CGTGGGCGAC AAGCCGGTGC TGGTGGAGCG CAAGGAGACG 
GACGATGATG AGGAGGAGTT CGACGACGAG GAAAACAACA GTGTAGTCCT GAGACACGAA 
ATACCATTTA CTAGCATATA CGGGCCGAGC GTCAAGTTCA ACTCGTTCCA GCGCAAGGTT 
TTCATCCCGG GCCGTGAGAT TCATGTTCGG ATCGTCGATA CGGAGCGTAG CGTCACTACA 
CATCTGCTAA ACCCCAATCT GTACACAATC GAGCTGACCC ACGGTCCCTT CAAGTGGACG 
ATCAAGCGGC GATACAAGCA CTTTAACTCG TTGCACCAGC AGCTCAGCTT TTTCCGCACC 
TCGCTCAACA TTCCTTTTCC CAGTCGCAGT CACAAGGAGA AGCGTACCAC TTTGAAAGCC 
ACAGCCAGAG AGATGGCTGA CGAGTCCACT CTAAAGGACC TTCCTTCTCA CACCAAGGTC 
AAACAAACTA GCACTCCGCT GAGGGCTGAA GGCAGAAGCA GTAAAATCGC GGGCAGTAAC 
GCCAACAATG CCATGGCTAT GATCAGTCCC AATCACAGCT CCATTCTGGC GGGTCTAACA 
CCACGACGCA TTCAAAAGAA GCGCAAAAAA AAGAAGAAAC GGAAGCTGCC GCGATTCCCA 
AACCGTCCTG AGAGTCTGGT CACCGTAGAG AATCTGAGCG TCAGAATAAA ACAGCTGGAG 
GACTACTTGT ACAACCTGCT GAACATCAGC TTGTACCGAT CTCACCATGA AACGCTAAAC 
TTCGTTGAAG TGTCTAATGT GTCCTTTGTT CCGGGAATGG GAATTAAGGG CAAGGAAGGC 
GTGATTTTAA AGCGAACTGG ATCAACGAGA CCAGGGCAAG CAGGATGCAA TTTTTTTGGG 
TGCTTTCAAA AGAACTGCTG TGTGCGCTGC AACTACTTTT GCTCCGACGT AGTTTGCGGC 
ACGTGGCGGA ACCGATGGTT TTTCGTAAAA GAGACCTGCT TCGGCTACAT CCGTCCAACA 
GACGGAAGCA TCCGGGCAGT GATCCTCTTT GATCAGGGCT TCGACGTTTC CACGGGTATC 
TATCAGACGG GCATGCGCAA GGGCTTGCAG GTACTGACGA ACAACCGTCA CATTGTGCTC 
AAGTGCTGGA CACGGCGTAA GTGTAAAGAG TGGATGCAAT ACCTCAAGAA CACGGCCAAC 
TCGTATGCGC GCGACTTCAC CCTGCCCAAT CCGCACATGT CCTTCGCTCC GATGCGCGCC 
AACACTCATG CCACGTGGTA TGTGGACGGC GCCCAGTATA TGTCAGCCGT GGCCGACGGC 
TTAGAGGCAG CCCTCGAGGA GATCTATATT GCCGACTGGT GGCTCAGTCC CGAGATATAC 
ATGAAGCGAC CCGCACTCGA CGGAGACTAC TGGCGATTGG ACAAGATCCT GTTGCGCAAG 
GCCGAACAGG GAGTGCGCGT CTTTGTGCTG CTCTACAAGG AGGTTGAAAT GGCACTTGGC 
ATAAACAGCT ACTACAGCAA GTCCACGCTG GCCAAGCATG AAAACATCAA GGTCATGCGT 
CATCCGGACC ATGCTAGAGG AGGTATTCTG CTTTGGGCAC ATCACGAAAA GATCGTCGTA 
ATCGACCAAA CCTATGCGTT TATGGGAGGT ATTGATTTGT GCTATGGACG TTGGGATGAT 
CACCACCATC GGCTAACGGA TCTGGGTAGC ATATCTACGT CATCTTTTTC TGGCAGCACG 
CGTCGAACGC CAAGTTTGTA CTTCACCAAA GACGACACGG ACTCAGCTTT CGGATCACGT 
AAGTCCTCGC GAAACGCTCA CTACGATACC TCCGCCAAGG AAAGGCCACC GTCCCCACCC 
CCGGATGAGC CCAATACTAG CATAGAGTTG AAAACTCTTA AGCCTGGTGA TCGACTGCTT 
ATACCGTCTA CGCTCGTTTC GAGTCCGGGT GAAACTCCCG CAGAATCGGG AATCGCTTTA 
GAAGGGATGA AACTCAACAC CCCTGAAATG GAGCGTAAGA ACGTACTCGA TCGCCTGAAG 
AACAACGCGA TGAAGGGCGC CCGTATGGGC AAGGACTTTA TGCACCGTCT AACAGCTACT 
GAGACGGAGG AAAAATCTGC GGAGGTGTAC ACTATCGAGT CCGAGGAAGC TACGGACCAC 
GAAGTCAACC TTAACATGGC TTCAGGTGGG CAGGAAGTGG CGATTACCAC TAGCAGTACA 
CAAATACTCA GTGAGTTCTG CGGCCAGGCC AAGTACTGGT TCGGCAAGGA TTACTCCAAC 
TTTATACTTA AAGACTGGAT GAACCTAAAC TCGCCGTTCG TGGATATCAT AGATCGAACA 
ACAACACCGC GGATGCCATG GCACGACGTG GGTCTGTGTG TGGTGGGTAC TTCCGCTAGG 
GATGTGGCCC GCCACTTCAT TCAGCGCTGG AATGCCATGA AGCTGGAGAA ACTACGCGAT 
AACACGAGAT TCCCCTATTT GATGCCAAAA AGCTATCACC AAGTGAGGCT CAATCCGAAC 
ATTCAGCAAA ACCGTCAGCA ACGGGTCACG TGCCAGCTAC TTCGAAGCGT CTCTGCCTGG 
AGCTGCGGCT TTATAGAGGC GGATCTTGTG GAGCAAAGCA TCCACGATGC CTACATCCAG 
ACGATCACCA AGGCGCAGCA CTACGTGTAC ATCGAAAACC AATTTTTTAT CACTATGCAG 
TTAGGCATGG GTGTGCCAGG TGCTTATAAC AATGTGCGGA ATCAAATCGG GGAAACACTC 
TTTAAACGGA TCGTTAGAGC GCACAAGGAA CGGAAGCCTT TCCGAGTTTA TGTGATTATG 
CCGCTCCTAC CGGGCTTTGA GGGTGATGTC GGTGGCAGTA CTGGGATAGC AGTCAGAGCA 
ATTACACACT GGAACTATGC GTCCATTTCC AGGGGACGCA CATCAATTTT GACCCGCCTG 
CAGGAGGCGG GTATTGCCAA TCCGGAAAAC TATATCTCAT TCCACAGCCT GCGCAACCAT 
TCTTTTTTGA ATAACACACC CATAACAGAG TTGATATATG TCCACTCAAA GCTCTTGATA 
GCCGACGATC GCGTTGTAAT CTGCGGTTCG GCAAACATTA ACGATCGCTC TATGATCGGA 
AAGCGGGACT CCGAGATAGC GGCTATTCTA ATGGACGAGG AGTTCGAGGA CGGACGCATG 
AATGGCAAGA AGTATCCGAG CGGAGTGTTT GCCGGTCGCC TTCGAAAATA CCTTTTTAAA 
GAACACTTAG GCCTCCTGGA AAGCGAAGGT TCCAGTCGGT CTGACCTGGA CATTAACGAT 
CCTGTTTGTG AGAAGTTTTG GCACGGCACC TGGCGTAGGA TTTCAATGCA GAACACAGAG 
ATTTACGACG AGGTGTTTAA GTGCATCCCC ACTGACTTTG TAAAAACCTT TGCCAGCCTT 
CGCAAATACC AGGAGGAGCC GCCTCTTGCC AAAACCGCCC CTGATCTAGC TGCCAACAGA 
GCCAACGACA TTCAGGGTTA CTTGGTCGAC CTGCCATTGG AATTTCTGAA CAAGGAGGTT 
CTCACGCCGC CTGGAACTAG TAAGGAGGGC CTAATCCCTA CCTCTGTATG GACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724469.2
CDS	336..4172
Translation	MADAGTVGGALPPYALTNSDEEHGSGEEDASEENSNNEEGEGVFRDCTDEAVVEHHNRCLPEFQFSLVDSEYDETLAFPDSVTILSNVGDKPVLVERKETDDDEEEFDDEENNSVVLRHEIPFTSIYGPSVKFNSFQRKVFIPGREIHVRIVDTERSVTTHLLNPNLYTIELTHGPFKWTIKRRYKHFNSLHQQLSFFRTSLNIPFPSRSHKEKRTTLKATAREMADESTLKDLPSHTKVKQTSTPLRAEGRSSKIAGSNANNAMAMISPNHSSILAGLTPRRIQKKRKKKKKRKLPRFPNRPESLVTVENLSVRIKQLEDYLYNLLNISLYRSHHETLNFVEVSNVSFVPGMGIKGKEGVILKRTGSTRPGQAGCNFFGCFQKNCCVRCNYFCSDVVCGTWRNRWFFVKETCFGYIRPTDGSIRAVILFDQGFDVSTGIYQTGMRKGLQVLTNNRHIVLKCWTRRKCKEWMQYLKNTANSYARDFTLPNPHMSFAPMRANTHATWYVDGAQYMSAVADGLEAALEEIYIADWWLSPEIYMKRPALDGDYWRLDKILLRKAEQGVRVFVLLYKEVEMALGINSYYSKSTLAKHENIKVMRHPDHARGGILLWAHHEKIVVIDQTYAFMGGIDLCYGRWDDHHHRLTDLGSISTSSFSGSTRRTPSLYFTKDDTDSAFGSRKSSRNAHYDTSAKERPPSPPPDEPNTSIELKTLKPGDRLLIPSTLVSSPGETPAESGIALEGMKLNTPEMERKNVLDRLKNNAMKGARMGKDFMHRLTATETEEKSAEVYTIESEEATDHEVNLNMASGGQEVAITTSSTQILSEFCGQAKYWFGKDYSNFILKDWMNLNSPFVDIIDRTTTPRMPWHDVGLCVVGTSARDVARHFIQRWNAMKLEKLRDNTRFPYLMPKSYHQVRLNPNIQQNRQQRVTCQLLRSVSAWSCGFIEADLVEQSIHDAYIQTITKAQHYVYIENQFFITMQLGMGVPGAYNNVRNQIGETLFKRIVRAHKERKPFRVYVIMPLLPGFEGDVGGSTGIAVRAITHWNYASISRGRTSILTRLQEAGIANPENYISFHSLRNHSFLNNTPITELIYVHSKLLIADDRVVICGSANINDRSMIGKRDSEIAAILMDEEFEDGRMNGKKYPSGVFAGRLRKYLFKEHLGLLESEGSSRSDLDINDPVCEKFWHGTWRRISMQNTEIYDEVFKCIPTDFVKTFASLRKYQEEPPLAKTAPDLAANRANDIQGYLVDLPLEFLNKEVLTPPGTSKEGLIPTSVWT

Target ORF information:

RefSeq Version	NM_165469.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phospholipase D, transcript variant A (Pld), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165469.4

ORF Insert Sequence:

ATGGCAGATG CCGGCACCGT TGGAGGCGCC TTGCCGCCCT ACGCACTTAC CAACTCGGAC 
GAAGAACATG GCAGCGGGGA AGAGGACGCG TCGGAGGAGA ACTCCAACAA TGAAGAGGGA 
GAGGGAGTGT TCCGGGACTG CACAGACGAA GCGGTTGTCG AGCATCATAA CCGCTGCCTG 
CCAGAATTTC AGTTCTCTCT AGTCGATTCT GAGTACGATG AGACCCTCGC TTTTCCTGAT 
TCTGTGACCA TTCTATCCAA CGTGGGCGAC AAGCCGGTGC TGGTGGAGCG CAAGGAGACG 
GACGATGATG AGGAGGAGTT CGACGACGAG GAAAACAACA GTGTAGTCCT GAGACACGAA 
ATACCATTTA CTAGCATATA CGGGCCGAGC GTCAAGTTCA ACTCGTTCCA GCGCAAGGTT 
TTCATCCCGG GCCGTGAGAT TCATGTTCGG ATCGTCGATA CGGAGCGTAG CGTCACTACA 
CATCTGCTAA ACCCCAATCT GTACACAATC GAGCTGACCC ACGGTCCCTT CAAGTGGACG 
ATCAAGCGGC GATACAAGCA CTTTAACTCG TTGCACCAGC AGCTCAGCTT TTTCCGCACC 
TCGCTCAACA TTCCTTTTCC CAGTCGCAGT CACAAGGAGA AGCGTACCAC TTTGAAAGCC 
ACAGCCAGAG AGATGGCTGA CGAGTCCACT CTAAAGGACC TTCCTTCTCA CACCAAGGTC 
AAACAAACTA GCACTCCGCT GAGGGCTGAA GGCAGAAGCA GTAAAATCGC GGGCAGTAAC 
GCCAACAATG CCATGGCTAT GATCAGTCCC AATCACAGCT CCATTCTGGC GGGTCTAACA 
CCACGACGCA TTCAAAAGAA GCGCAAAAAA AAGAAGAAAC GGAAGCTGCC GCGATTCCCA 
AACCGTCCTG AGAGTCTGGT CACCGTAGAG AATCTGAGCG TCAGAATAAA ACAGCTGGAG 
GACTACTTGT ACAACCTGCT GAACATCAGC TTGTACCGAT CTCACCATGA AACGCTAAAC 
TTCGTTGAAG TGTCTAATGT GTCCTTTGTT CCGGGAATGG GAATTAAGGG CAAGGAAGGC 
GTGATTTTAA AGCGAACTGG ATCAACGAGA CCAGGGCAAG CAGGATGCAA TTTTTTTGGG 
TGCTTTCAAA AGAACTGCTG TGTGCGCTGC AACTACTTTT GCTCCGACGT AGTTTGCGGC 
ACGTGGCGGA ACCGATGGTT TTTCGTAAAA GAGACCTGCT TCGGCTACAT CCGTCCAACA 
GACGGAAGCA TCCGGGCAGT GATCCTCTTT GATCAGGGCT TCGACGTTTC CACGGGTATC 
TATCAGACGG GCATGCGCAA GGGCTTGCAG GTACTGACGA ACAACCGTCA CATTGTGCTC 
AAGTGCTGGA CACGGCGTAA GTGTAAAGAG TGGATGCAAT ACCTCAAGAA CACGGCCAAC 
TCGTATGCGC GCGACTTCAC CCTGCCCAAT CCGCACATGT CCTTCGCTCC GATGCGCGCC 
AACACTCATG CCACGTGGTA TGTGGACGGC GCCCAGTATA TGTCAGCCGT GGCCGACGGC 
TTAGAGGCAG CCCTCGAGGA GATCTATATT GCCGACTGGT GGCTCAGTCC CGAGATATAC 
ATGAAGCGAC CCGCACTCGA CGGAGACTAC TGGCGATTGG ACAAGATCCT GTTGCGCAAG 
GCCGAACAGG GAGTGCGCGT CTTTGTGCTG CTCTACAAGG AGGTTGAAAT GGCACTTGGC 
ATAAACAGCT ACTACAGCAA GTCCACGCTG GCCAAGCATG AAAACATCAA GGTCATGCGT 
CATCCGGACC ATGCTAGAGG AGGTATTCTG CTTTGGGCAC ATCACGAAAA GATCGTCGTA 
ATCGACCAAA CCTATGCGTT TATGGGAGGT ATTGATTTGT GCTATGGACG TTGGGATGAT 
CACCACCATC GGCTAACGGA TCTGGGTAGC ATATCTACGT CATCTTTTTC TGGCAGCACG 
CGTCGAACGC CAAGTTTGTA CTTCACCAAA GACGACACGG ACTCAGCTTT CGGATCACGT 
AAGTCCTCGC GAAACGCTCA CTACGATACC TCCGCCAAGG AAAGGCCACC GTCCCCACCC 
CCGGATGAGC CCAATACTAG CATAGAGTTG AAAACTCTTA AGCCTGGTGA TCGACTGCTT 
ATACCGTCTA CGCTCGTTTC GAGTCCGGGT GAAACTCCCG CAGAATCGGG AATCGCTTTA 
GAAGGGATGA AACTCAACAC CCCTGAAATG GAGCGTAAGA ACGTACTCGA TCGCCTGAAG 
AACAACGCGA TGAAGGGCGC CCGTATGGGC AAGGACTTTA TGCACCGTCT AACAGCTACT 
GAGACGGAGG AAAAATCTGC GGAGGTGTAC ACTATCGAGT CCGAGGAAGC TACGGACCAC 
GAAGTCAACC TTAACATGGC TTCAGGTGGG CAGGAAGTGG CGATTACCAC TAGCAGTACA 
CAAATACTCA GTGAGTTCTG CGGCCAGGCC AAGTACTGGT TCGGCAAGGA TTACTCCAAC 
TTTATACTTA AAGACTGGAT GAACCTAAAC TCGCCGTTCG TGGATATCAT AGATCGAACA 
ACAACACCGC GGATGCCATG GCACGACGTG GGTCTGTGTG TGGTGGGTAC TTCCGCTAGG 
GATGTGGCCC GCCACTTCAT TCAGCGCTGG AATGCCATGA AGCTGGAGAA ACTACGCGAT 
AACACGAGAT TCCCCTATTT GATGCCAAAA AGCTATCACC AAGTGAGGCT CAATCCGAAC 
ATTCAGCAAA ACCGTCAGCA ACGGGTCACG TGCCAGCTAC TTCGAAGCGT CTCTGCCTGG 
AGCTGCGGCT TTATAGAGGC GGATCTTGTG GAGCAAAGCA TCCACGATGC CTACATCCAG 
ACGATCACCA AGGCGCAGCA CTACGTGTAC ATCGAAAACC AATTTTTTAT CACTATGCAG 
TTAGGCATGG GTGTGCCAGG TGCTTATAAC AATGTGCGGA ATCAAATCGG GGAAACACTC 
TTTAAACGGA TCGTTAGAGC GCACAAGGAA CGGAAGCCTT TCCGAGTTTA TGTGATTATG 
CCGCTCCTAC CGGGCTTTGA GGGTGATGTC GGTGGCAGTA CTGGGATAGC AGTCAGAGCA 
ATTACACACT GGAACTATGC GTCCATTTCC AGGGGACGCA CATCAATTTT GACCCGCCTG 
CAGGAGGCGG GTATTGCCAA TCCGGAAAAC TATATCTCAT TCCACAGCCT GCGCAACCAT 
TCTTTTTTGA ATAACACACC CATAACAGAG TTGATATATG TCCACTCAAA GCTCTTGATA 
GCCGACGATC GCGTTGTAAT CTGCGGTTCG GCAAACATTA ACGATCGCTC TATGATCGGA 
AAGCGGGACT CCGAGATAGC GGCTATTCTA ATGGACGAGG AGTTCGAGGA CGGACGCATG 
AATGGCAAGA AGTATCCGAG CGGAGTGTTT GCCGGTCGCC TTCGAAAATA CCTTTTTAAA 
GAACACTTAG GCCTCCTGGA AAGCGAAGGT TCCAGTCGGT CTGACCTGGA CATTAACGAT 
CCTGTTTGTG AGAAGTTTTG GCACGGCACC TGGCGTAGGA TTTCAATGCA GAACACAGAG 
ATTTACGACG AGGTGTTTAA GTGCATCCCC ACTGACTTTG TAAAAACCTT TGCCAGCCTT 
CGCAAATACC AGGAGGAGCC GCCTCTTGCC AAAACCGCCC CTGATCTAGC TGCCAACAGA 
GCCAACGACA TTCAGGGTTA CTTGGTCGAC CTGCCATTGG AATTTCTGAA CAAGGAGGTT 
CTCACGCCGC CTGGAACTAG TAAGGAGGGC CTAATCCCTA CCTCTGTATG GACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pld ( NM_165471.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724471.2 Drosophila melanogaster phospholipase D, transcript variant C (Pld), mRNA.

Price & Availability↑

CloneID	OFa09021
Clone ID Related Accession (Same CDS sequence)	NM_165470.3 , NM_078903.4 , NM_165469.4 , NM_165471.3
Accession Version	NM_165471.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phospholipase D, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165471.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGATG CCGGCACCGT TGGAGGCGCC TTGCCGCCCT ACGCACTTAC CAACTCGGAC 
GAAGAACATG GCAGCGGGGA AGAGGACGCG TCGGAGGAGA ACTCCAACAA TGAAGAGGGA 
GAGGGAGTGT TCCGGGACTG CACAGACGAA GCGGTTGTCG AGCATCATAA CCGCTGCCTG 
CCAGAATTTC AGTTCTCTCT AGTCGATTCT GAGTACGATG AGACCCTCGC TTTTCCTGAT 
TCTGTGACCA TTCTATCCAA CGTGGGCGAC AAGCCGGTGC TGGTGGAGCG CAAGGAGACG 
GACGATGATG AGGAGGAGTT CGACGACGAG GAAAACAACA GTGTAGTCCT GAGACACGAA 
ATACCATTTA CTAGCATATA CGGGCCGAGC GTCAAGTTCA ACTCGTTCCA GCGCAAGGTT 
TTCATCCCGG GCCGTGAGAT TCATGTTCGG ATCGTCGATA CGGAGCGTAG CGTCACTACA 
CATCTGCTAA ACCCCAATCT GTACACAATC GAGCTGACCC ACGGTCCCTT CAAGTGGACG 
ATCAAGCGGC GATACAAGCA CTTTAACTCG TTGCACCAGC AGCTCAGCTT TTTCCGCACC 
TCGCTCAACA TTCCTTTTCC CAGTCGCAGT CACAAGGAGA AGCGTACCAC TTTGAAAGCC 
ACAGCCAGAG AGATGGCTGA CGAGTCCACT CTAAAGGACC TTCCTTCTCA CACCAAGGTC 
AAACAAACTA GCACTCCGCT GAGGGCTGAA GGCAGAAGCA GTAAAATCGC GGGCAGTAAC 
GCCAACAATG CCATGGCTAT GATCAGTCCC AATCACAGCT CCATTCTGGC GGGTCTAACA 
CCACGACGCA TTCAAAAGAA GCGCAAAAAA AAGAAGAAAC GGAAGCTGCC GCGATTCCCA 
AACCGTCCTG AGAGTCTGGT CACCGTAGAG AATCTGAGCG TCAGAATAAA ACAGCTGGAG 
GACTACTTGT ACAACCTGCT GAACATCAGC TTGTACCGAT CTCACCATGA AACGCTAAAC 
TTCGTTGAAG TGTCTAATGT GTCCTTTGTT CCGGGAATGG GAATTAAGGG CAAGGAAGGC 
GTGATTTTAA AGCGAACTGG ATCAACGAGA CCAGGGCAAG CAGGATGCAA TTTTTTTGGG 
TGCTTTCAAA AGAACTGCTG TGTGCGCTGC AACTACTTTT GCTCCGACGT AGTTTGCGGC 
ACGTGGCGGA ACCGATGGTT TTTCGTAAAA GAGACCTGCT TCGGCTACAT CCGTCCAACA 
GACGGAAGCA TCCGGGCAGT GATCCTCTTT GATCAGGGCT TCGACGTTTC CACGGGTATC 
TATCAGACGG GCATGCGCAA GGGCTTGCAG GTACTGACGA ACAACCGTCA CATTGTGCTC 
AAGTGCTGGA CACGGCGTAA GTGTAAAGAG TGGATGCAAT ACCTCAAGAA CACGGCCAAC 
TCGTATGCGC GCGACTTCAC CCTGCCCAAT CCGCACATGT CCTTCGCTCC GATGCGCGCC 
AACACTCATG CCACGTGGTA TGTGGACGGC GCCCAGTATA TGTCAGCCGT GGCCGACGGC 
TTAGAGGCAG CCCTCGAGGA GATCTATATT GCCGACTGGT GGCTCAGTCC CGAGATATAC 
ATGAAGCGAC CCGCACTCGA CGGAGACTAC TGGCGATTGG ACAAGATCCT GTTGCGCAAG 
GCCGAACAGG GAGTGCGCGT CTTTGTGCTG CTCTACAAGG AGGTTGAAAT GGCACTTGGC 
ATAAACAGCT ACTACAGCAA GTCCACGCTG GCCAAGCATG AAAACATCAA GGTCATGCGT 
CATCCGGACC ATGCTAGAGG AGGTATTCTG CTTTGGGCAC ATCACGAAAA GATCGTCGTA 
ATCGACCAAA CCTATGCGTT TATGGGAGGT ATTGATTTGT GCTATGGACG TTGGGATGAT 
CACCACCATC GGCTAACGGA TCTGGGTAGC ATATCTACGT CATCTTTTTC TGGCAGCACG 
CGTCGAACGC CAAGTTTGTA CTTCACCAAA GACGACACGG ACTCAGCTTT CGGATCACGT 
AAGTCCTCGC GAAACGCTCA CTACGATACC TCCGCCAAGG AAAGGCCACC GTCCCCACCC 
CCGGATGAGC CCAATACTAG CATAGAGTTG AAAACTCTTA AGCCTGGTGA TCGACTGCTT 
ATACCGTCTA CGCTCGTTTC GAGTCCGGGT GAAACTCCCG CAGAATCGGG AATCGCTTTA 
GAAGGGATGA AACTCAACAC CCCTGAAATG GAGCGTAAGA ACGTACTCGA TCGCCTGAAG 
AACAACGCGA TGAAGGGCGC CCGTATGGGC AAGGACTTTA TGCACCGTCT AACAGCTACT 
GAGACGGAGG AAAAATCTGC GGAGGTGTAC ACTATCGAGT CCGAGGAAGC TACGGACCAC 
GAAGTCAACC TTAACATGGC TTCAGGTGGG CAGGAAGTGG CGATTACCAC TAGCAGTACA 
CAAATACTCA GTGAGTTCTG CGGCCAGGCC AAGTACTGGT TCGGCAAGGA TTACTCCAAC 
TTTATACTTA AAGACTGGAT GAACCTAAAC TCGCCGTTCG TGGATATCAT AGATCGAACA 
ACAACACCGC GGATGCCATG GCACGACGTG GGTCTGTGTG TGGTGGGTAC TTCCGCTAGG 
GATGTGGCCC GCCACTTCAT TCAGCGCTGG AATGCCATGA AGCTGGAGAA ACTACGCGAT 
AACACGAGAT TCCCCTATTT GATGCCAAAA AGCTATCACC AAGTGAGGCT CAATCCGAAC 
ATTCAGCAAA ACCGTCAGCA ACGGGTCACG TGCCAGCTAC TTCGAAGCGT CTCTGCCTGG 
AGCTGCGGCT TTATAGAGGC GGATCTTGTG GAGCAAAGCA TCCACGATGC CTACATCCAG 
ACGATCACCA AGGCGCAGCA CTACGTGTAC ATCGAAAACC AATTTTTTAT CACTATGCAG 
TTAGGCATGG GTGTGCCAGG TGCTTATAAC AATGTGCGGA ATCAAATCGG GGAAACACTC 
TTTAAACGGA TCGTTAGAGC GCACAAGGAA CGGAAGCCTT TCCGAGTTTA TGTGATTATG 
CCGCTCCTAC CGGGCTTTGA GGGTGATGTC GGTGGCAGTA CTGGGATAGC AGTCAGAGCA 
ATTACACACT GGAACTATGC GTCCATTTCC AGGGGACGCA CATCAATTTT GACCCGCCTG 
CAGGAGGCGG GTATTGCCAA TCCGGAAAAC TATATCTCAT TCCACAGCCT GCGCAACCAT 
TCTTTTTTGA ATAACACACC CATAACAGAG TTGATATATG TCCACTCAAA GCTCTTGATA 
GCCGACGATC GCGTTGTAAT CTGCGGTTCG GCAAACATTA ACGATCGCTC TATGATCGGA 
AAGCGGGACT CCGAGATAGC GGCTATTCTA ATGGACGAGG AGTTCGAGGA CGGACGCATG 
AATGGCAAGA AGTATCCGAG CGGAGTGTTT GCCGGTCGCC TTCGAAAATA CCTTTTTAAA 
GAACACTTAG GCCTCCTGGA AAGCGAAGGT TCCAGTCGGT CTGACCTGGA CATTAACGAT 
CCTGTTTGTG AGAAGTTTTG GCACGGCACC TGGCGTAGGA TTTCAATGCA GAACACAGAG 
ATTTACGACG AGGTGTTTAA GTGCATCCCC ACTGACTTTG TAAAAACCTT TGCCAGCCTT 
CGCAAATACC AGGAGGAGCC GCCTCTTGCC AAAACCGCCC CTGATCTAGC TGCCAACAGA 
GCCAACGACA TTCAGGGTTA CTTGGTCGAC CTGCCATTGG AATTTCTGAA CAAGGAGGTT 
CTCACGCCGC CTGGAACTAG TAAGGAGGGC CTAATCCCTA CCTCTGTATG GACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724471.2
CDS	548..4384
Translation	MADAGTVGGALPPYALTNSDEEHGSGEEDASEENSNNEEGEGVFRDCTDEAVVEHHNRCLPEFQFSLVDSEYDETLAFPDSVTILSNVGDKPVLVERKETDDDEEEFDDEENNSVVLRHEIPFTSIYGPSVKFNSFQRKVFIPGREIHVRIVDTERSVTTHLLNPNLYTIELTHGPFKWTIKRRYKHFNSLHQQLSFFRTSLNIPFPSRSHKEKRTTLKATAREMADESTLKDLPSHTKVKQTSTPLRAEGRSSKIAGSNANNAMAMISPNHSSILAGLTPRRIQKKRKKKKKRKLPRFPNRPESLVTVENLSVRIKQLEDYLYNLLNISLYRSHHETLNFVEVSNVSFVPGMGIKGKEGVILKRTGSTRPGQAGCNFFGCFQKNCCVRCNYFCSDVVCGTWRNRWFFVKETCFGYIRPTDGSIRAVILFDQGFDVSTGIYQTGMRKGLQVLTNNRHIVLKCWTRRKCKEWMQYLKNTANSYARDFTLPNPHMSFAPMRANTHATWYVDGAQYMSAVADGLEAALEEIYIADWWLSPEIYMKRPALDGDYWRLDKILLRKAEQGVRVFVLLYKEVEMALGINSYYSKSTLAKHENIKVMRHPDHARGGILLWAHHEKIVVIDQTYAFMGGIDLCYGRWDDHHHRLTDLGSISTSSFSGSTRRTPSLYFTKDDTDSAFGSRKSSRNAHYDTSAKERPPSPPPDEPNTSIELKTLKPGDRLLIPSTLVSSPGETPAESGIALEGMKLNTPEMERKNVLDRLKNNAMKGARMGKDFMHRLTATETEEKSAEVYTIESEEATDHEVNLNMASGGQEVAITTSSTQILSEFCGQAKYWFGKDYSNFILKDWMNLNSPFVDIIDRTTTPRMPWHDVGLCVVGTSARDVARHFIQRWNAMKLEKLRDNTRFPYLMPKSYHQVRLNPNIQQNRQQRVTCQLLRSVSAWSCGFIEADLVEQSIHDAYIQTITKAQHYVYIENQFFITMQLGMGVPGAYNNVRNQIGETLFKRIVRAHKERKPFRVYVIMPLLPGFEGDVGGSTGIAVRAITHWNYASISRGRTSILTRLQEAGIANPENYISFHSLRNHSFLNNTPITELIYVHSKLLIADDRVVICGSANINDRSMIGKRDSEIAAILMDEEFEDGRMNGKKYPSGVFAGRLRKYLFKEHLGLLESEGSSRSDLDINDPVCEKFWHGTWRRISMQNTEIYDEVFKCIPTDFVKTFASLRKYQEEPPLAKTAPDLAANRANDIQGYLVDLPLEFLNKEVLTPPGTSKEGLIPTSVWT

Target ORF information:

RefSeq Version	NM_165471.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phospholipase D, transcript variant C (Pld), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165471.3

ORF Insert Sequence:

ATGGCAGATG CCGGCACCGT TGGAGGCGCC TTGCCGCCCT ACGCACTTAC CAACTCGGAC 
GAAGAACATG GCAGCGGGGA AGAGGACGCG TCGGAGGAGA ACTCCAACAA TGAAGAGGGA 
GAGGGAGTGT TCCGGGACTG CACAGACGAA GCGGTTGTCG AGCATCATAA CCGCTGCCTG 
CCAGAATTTC AGTTCTCTCT AGTCGATTCT GAGTACGATG AGACCCTCGC TTTTCCTGAT 
TCTGTGACCA TTCTATCCAA CGTGGGCGAC AAGCCGGTGC TGGTGGAGCG CAAGGAGACG 
GACGATGATG AGGAGGAGTT CGACGACGAG GAAAACAACA GTGTAGTCCT GAGACACGAA 
ATACCATTTA CTAGCATATA CGGGCCGAGC GTCAAGTTCA ACTCGTTCCA GCGCAAGGTT 
TTCATCCCGG GCCGTGAGAT TCATGTTCGG ATCGTCGATA CGGAGCGTAG CGTCACTACA 
CATCTGCTAA ACCCCAATCT GTACACAATC GAGCTGACCC ACGGTCCCTT CAAGTGGACG 
ATCAAGCGGC GATACAAGCA CTTTAACTCG TTGCACCAGC AGCTCAGCTT TTTCCGCACC 
TCGCTCAACA TTCCTTTTCC CAGTCGCAGT CACAAGGAGA AGCGTACCAC TTTGAAAGCC 
ACAGCCAGAG AGATGGCTGA CGAGTCCACT CTAAAGGACC TTCCTTCTCA CACCAAGGTC 
AAACAAACTA GCACTCCGCT GAGGGCTGAA GGCAGAAGCA GTAAAATCGC GGGCAGTAAC 
GCCAACAATG CCATGGCTAT GATCAGTCCC AATCACAGCT CCATTCTGGC GGGTCTAACA 
CCACGACGCA TTCAAAAGAA GCGCAAAAAA AAGAAGAAAC GGAAGCTGCC GCGATTCCCA 
AACCGTCCTG AGAGTCTGGT CACCGTAGAG AATCTGAGCG TCAGAATAAA ACAGCTGGAG 
GACTACTTGT ACAACCTGCT GAACATCAGC TTGTACCGAT CTCACCATGA AACGCTAAAC 
TTCGTTGAAG TGTCTAATGT GTCCTTTGTT CCGGGAATGG GAATTAAGGG CAAGGAAGGC 
GTGATTTTAA AGCGAACTGG ATCAACGAGA CCAGGGCAAG CAGGATGCAA TTTTTTTGGG 
TGCTTTCAAA AGAACTGCTG TGTGCGCTGC AACTACTTTT GCTCCGACGT AGTTTGCGGC 
ACGTGGCGGA ACCGATGGTT TTTCGTAAAA GAGACCTGCT TCGGCTACAT CCGTCCAACA 
GACGGAAGCA TCCGGGCAGT GATCCTCTTT GATCAGGGCT TCGACGTTTC CACGGGTATC 
TATCAGACGG GCATGCGCAA GGGCTTGCAG GTACTGACGA ACAACCGTCA CATTGTGCTC 
AAGTGCTGGA CACGGCGTAA GTGTAAAGAG TGGATGCAAT ACCTCAAGAA CACGGCCAAC 
TCGTATGCGC GCGACTTCAC CCTGCCCAAT CCGCACATGT CCTTCGCTCC GATGCGCGCC 
AACACTCATG CCACGTGGTA TGTGGACGGC GCCCAGTATA TGTCAGCCGT GGCCGACGGC 
TTAGAGGCAG CCCTCGAGGA GATCTATATT GCCGACTGGT GGCTCAGTCC CGAGATATAC 
ATGAAGCGAC CCGCACTCGA CGGAGACTAC TGGCGATTGG ACAAGATCCT GTTGCGCAAG 
GCCGAACAGG GAGTGCGCGT CTTTGTGCTG CTCTACAAGG AGGTTGAAAT GGCACTTGGC 
ATAAACAGCT ACTACAGCAA GTCCACGCTG GCCAAGCATG AAAACATCAA GGTCATGCGT 
CATCCGGACC ATGCTAGAGG AGGTATTCTG CTTTGGGCAC ATCACGAAAA GATCGTCGTA 
ATCGACCAAA CCTATGCGTT TATGGGAGGT ATTGATTTGT GCTATGGACG TTGGGATGAT 
CACCACCATC GGCTAACGGA TCTGGGTAGC ATATCTACGT CATCTTTTTC TGGCAGCACG 
CGTCGAACGC CAAGTTTGTA CTTCACCAAA GACGACACGG ACTCAGCTTT CGGATCACGT 
AAGTCCTCGC GAAACGCTCA CTACGATACC TCCGCCAAGG AAAGGCCACC GTCCCCACCC 
CCGGATGAGC CCAATACTAG CATAGAGTTG AAAACTCTTA AGCCTGGTGA TCGACTGCTT 
ATACCGTCTA CGCTCGTTTC GAGTCCGGGT GAAACTCCCG CAGAATCGGG AATCGCTTTA 
GAAGGGATGA AACTCAACAC CCCTGAAATG GAGCGTAAGA ACGTACTCGA TCGCCTGAAG 
AACAACGCGA TGAAGGGCGC CCGTATGGGC AAGGACTTTA TGCACCGTCT AACAGCTACT 
GAGACGGAGG AAAAATCTGC GGAGGTGTAC ACTATCGAGT CCGAGGAAGC TACGGACCAC 
GAAGTCAACC TTAACATGGC TTCAGGTGGG CAGGAAGTGG CGATTACCAC TAGCAGTACA 
CAAATACTCA GTGAGTTCTG CGGCCAGGCC AAGTACTGGT TCGGCAAGGA TTACTCCAAC 
TTTATACTTA AAGACTGGAT GAACCTAAAC TCGCCGTTCG TGGATATCAT AGATCGAACA 
ACAACACCGC GGATGCCATG GCACGACGTG GGTCTGTGTG TGGTGGGTAC TTCCGCTAGG 
GATGTGGCCC GCCACTTCAT TCAGCGCTGG AATGCCATGA AGCTGGAGAA ACTACGCGAT 
AACACGAGAT TCCCCTATTT GATGCCAAAA AGCTATCACC AAGTGAGGCT CAATCCGAAC 
ATTCAGCAAA ACCGTCAGCA ACGGGTCACG TGCCAGCTAC TTCGAAGCGT CTCTGCCTGG 
AGCTGCGGCT TTATAGAGGC GGATCTTGTG GAGCAAAGCA TCCACGATGC CTACATCCAG 
ACGATCACCA AGGCGCAGCA CTACGTGTAC ATCGAAAACC AATTTTTTAT CACTATGCAG 
TTAGGCATGG GTGTGCCAGG TGCTTATAAC AATGTGCGGA ATCAAATCGG GGAAACACTC 
TTTAAACGGA TCGTTAGAGC GCACAAGGAA CGGAAGCCTT TCCGAGTTTA TGTGATTATG 
CCGCTCCTAC CGGGCTTTGA GGGTGATGTC GGTGGCAGTA CTGGGATAGC AGTCAGAGCA 
ATTACACACT GGAACTATGC GTCCATTTCC AGGGGACGCA CATCAATTTT GACCCGCCTG 
CAGGAGGCGG GTATTGCCAA TCCGGAAAAC TATATCTCAT TCCACAGCCT GCGCAACCAT 
TCTTTTTTGA ATAACACACC CATAACAGAG TTGATATATG TCCACTCAAA GCTCTTGATA 
GCCGACGATC GCGTTGTAAT CTGCGGTTCG GCAAACATTA ACGATCGCTC TATGATCGGA 
AAGCGGGACT CCGAGATAGC GGCTATTCTA ATGGACGAGG AGTTCGAGGA CGGACGCATG 
AATGGCAAGA AGTATCCGAG CGGAGTGTTT GCCGGTCGCC TTCGAAAATA CCTTTTTAAA 
GAACACTTAG GCCTCCTGGA AAGCGAAGGT TCCAGTCGGT CTGACCTGGA CATTAACGAT 
CCTGTTTGTG AGAAGTTTTG GCACGGCACC TGGCGTAGGA TTTCAATGCA GAACACAGAG 
ATTTACGACG AGGTGTTTAA GTGCATCCCC ACTGACTTTG TAAAAACCTT TGCCAGCCTT 
CGCAAATACC AGGAGGAGCC GCCTCTTGCC AAAACCGCCC CTGATCTAGC TGCCAACAGA 
GCCAACGACA TTCAGGGTTA CTTGGTCGAC CTGCCATTGG AATTTCTGAA CAAGGAGGTT 
CTCACGCCGC CTGGAACTAG TAAGGAGGGC CTAATCCCTA CCTCTGTATG GACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pld ( NM_165472.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724472.3 Drosophila melanogaster phospholipase D, transcript variant G (Pld), mRNA.

Price & Availability↑

CloneID	OFa09023
Clone ID Related Accession (Same CDS sequence)	NM_165472.3
Accession Version	NM_165472.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phospholipase D, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165472.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGATG CCGGCACCGT TGGAGGCGCC TTGCCGCCCT ACGCACTTAC CAACTCGGAC 
GAAGAACATG GCAGCGGGGA AGAGGACGCG TCGGAGGAGA ACTCCAACAA TGAAGAGGGA 
GAGGGAGTGT TCCGGGACTG CACAGACGAA GCGGTTGTCG AGCATCATAA CCGCTGCCTG 
CCAGAATTTC AGTTCTCTCT AGTCGATTCT GAGTACGATG AGACCCTCGC TTTTCCTGAT 
TCTGTGACCA TTCTATCCAA CGTGGGCGAC AAGCCGGTGC TGGTGGAGCG CAAGGAGACG 
GACGATGATG AGGAGGAGTT CGACGACGAG GAAAACAACA GTGTAGTCCT GAGACACGAA 
ATACCATTTA CTAGCATATA CGGGCCGAGC GTCAAGTTCA ACTCGTTCCA GCGCAAGGTT 
TTCATCCCGG GCCGTGAGAT TCATGTTCGG ATCGTCGATA CGGAGCGTAG CGTCACTACA 
CATCTGCTAA ACCCCAATCT GTACACAATC GAGCTGACCC ACGGTCCCTT CAAGTGGACG 
ATCAAGCGGC GATACAAGCA CTTTAACTCG TTGCACCAGC AGCTCAGCTT TTTCCGCACC 
TCGCTCAACA TTCCTTTTCC CAGTCGCAGT CACAAGGAGA AGCGTACCAC TTTGAAAGCC 
ACAGCCAGAG AGATGGCTGA CGAGTCCACT CTAAAGGACC TTCCTTCTCA CACCAAGGTC 
AAACAAACTA GCACTCCGCT GAGGGCTGAA GGCAGAAGCA GTAAAATCGC GGGCAGTAAC 
GCCAACAATG CCATGGCTAT GATCAGTCCC AATCACAGCT CCATTCTGGC GGGTCTAACA 
CCACGACGCA TTCAAAAGAA GCGCAAAAAA AAGAAGAAAC GGAAGCTGCC GCGATTCCCA 
AACCGTCCTG AGAGTCTGGT CACCGTAGAG AATCTGAGCG TCAGAATAAA ACAGCTGGAG 
GACTACTTGT ACAACCTGCT GAACATCAGC TTGTACCGAT CTCACCATGA AACGGGCAAG 
GAAGGCGTGA TTTTAAAGCG AACTGGATCA ACGAGACCAG GGCAAGCAGG ATGCAATTTT 
TTTGGGTGCT TTCAAAAGAA CTGCTGTGTG CGCTGCAACT ACTTTTGCTC CGACGTAGTT 
TGCGGCACGT GGCGGAACCG ATGGTTTTTC GTAAAAGAGA CCTGCTTCGG CTACATCCGT 
CCAACAGACG GAAGCATCCG GGCAGTGATC CTCTTTGATC AGGGCTTCGA CGTTTCCACG 
GGTATCTATC AGACGGGCAT GCGCAAGGGC TTGCAGGTAC TGACGAACAA CCGTCACATT 
GTGCTCAAGT GCTGGACACG GCGTAAGTGT AAAGAGTGGA TGCAATACCT CAAGAACACG 
GCCAACTCGT ATGCGCGCGA CTTCACCCTG CCCAATCCGC ACATGTCCTT CGCTCCGATG 
CGCGCCAACA CTCATGCCAC GTGGTATGTG GACGGCGCCC AGTATATGTC AGCCGTGGCC 
GACGGCTTAG AGGCAGCCCT CGAGGAGATC TATATTGCCG ACTGGTGGCT CAGTCCCGAG 
ATATACATGA AGCGACCCGC ACTCGACGGA GACTACTGGC GATTGGACAA GATCCTGTTG 
CGCAAGGCCG AACAGGGAGT GCGCGTCTTT GTGCTGCTCT ACAAGGAGGT TGAAATGGCA 
CTTGGCATAA ACAGCTACTA CAGCAAGTCC ACGCTGGCCA AGCATGAAAA CATCAAGGTC 
ATGCGTCATC CGGACCATGC TAGAGGAGGT ATTCTGCTTT GGGCACATCA CGAAAAGATC 
GTCGTAATCG ACCAAACCTA TGCGTTTATG GGAGGTATTG ATTTGTGCTA TGGACGTTGG 
GATGATCACC ACCATCGGCT AACGGATCTG GGTAGCATAT CTACGTCATC TTTTTCTGGC 
AGCACGCGTC GAACGCCAAG TTTGTACTTC ACCAAAGACG ACACGGACTC AGCTTTCGGA 
TCACGTAAGT CCTCGCGAAA CGCTCACTAC GATACCTCCG CCAAGGAAAG GCCACCGTCC 
CCACCCCCGG ATGAGCCCAA TACTAGCATA GAGTTGAAAA CTCTTAAGCC TGGTGATCGA 
CTGCTTATAC CGTCTACGCT CGTTTCGAGT CCGGGTGAAA CTCCCGCAGA ATCGGGAATC 
GCTTTAGAAG GGATGAAACT CAACACCCCT GAAATGGAGC GTAAGAACGT ACTCGATCGC 
CTGAAGAACA ACGCGATGAA GGGCGCCCGT ATGGGCAAGG ACTTTATGCA CCGTCTAACA 
GCTACTGAGA CGGAGGAAAA ATCTGCGGAG GTGTACACTA TCGAGTCCGA GGAAGCTACG 
GACCACGAAG TCAACCTTAA CATGGCTTCA GGTGGGCAGG AAGTGGCGAT TACCACTAGC 
AGTACACAAA TACTCAGTGA GTTCTGCGGC CAGGCCAAGT ACTGGTTCGG CAAGGATTAC 
TCCAACTTTA TACTTAAAGA CTGGATGAAC CTAAACTCGC CGTTCGTGGA TATCATAGAT 
CGAACAACAA CACCGCGGAT GCCATGGCAC GACGTGGGTC TGTGTGTGGT GGGTACTTCC 
GCTAGGGATG TGGCCCGCCA CTTCATTCAG CGCTGGAATG CCATGAAGCT GGAGAAACTA 
CGCGATAACA CGAGATTCCC CTATTTGATG CCAAAAAGCT ATCACCAAGT GAGGCTCAAT 
CCGAACATTC AGCAAAACCG TCAGCAACGG GTCACGTGCC AGCTACTTCG AAGCGTCTCT 
GCCTGGAGCT GCGGCTTTAT AGAGGCGGAT CTTGTGGAGC AAAGCATCCA CGATGCCTAC 
ATCCAGACGA TCACCAAGGC GCAGCACTAC GTGTACATCG AAAACCAATT TTTTATCACT 
ATGCAGTTAG GCATGGGTGT GCCAGGTGCT TATAACAATG TGCGGAATCA AATCGGGGAA 
ACACTCTTTA AACGGATCGT TAGAGCGCAC AAGGAACGGA AGCCTTTCCG AGTTTATGTG 
ATTATGCCGC TCCTACCGGG CTTTGAGGGT GATGTCGGTG GCAGTACTGG GATAGCAGTC 
AGAGCAATTA CACACTGGAA CTATGCGTCC ATTTCCAGGG GACGCACATC AATTTTGACC 
CGCCTGCAGG AGGCGGGTAT TGCCAATCCG GAAAACTATA TCTCATTCCA CAGCCTGCGC 
AACCATTCTT TTTTGAATAA CACACCCATA ACAGAGTTGA TATATGTCCA CTCAAAGCTC 
TTGATAGCCG ACGATCGCGT TGTAATCTGC GGTTCGGCAA ACATTAACGA TCGCTCTATG 
ATCGGAAAGC GGGACTCCGA GATAGCGGCT ATTCTAATGG ACGAGGAGTT CGAGGACGGA 
CGCATGAATG GCAAGAAGTA TCCGAGCGGA GTGTTTGCCG GTCGCCTTCG AAAATACCTT 
TTTAAAGAAC ACTTAGGCCT CCTGGAAAGC GAAGGTTCCA GTCGGTCTGA CCTGGACATT 
AACGATCCTG TTTGTGAGAA GTTTTGGCAC GGCACCTGGC GTAGGATTTC AATGCAGAAC 
ACAGAGATTT ACGACGAGGT GTTTAAGTGC ATCCCCACTG ACTTTGTAAA AACCTTTGCC 
AGCCTTCGCA AATACCAGGA GGAGCCGCCT CTTGCCAAAA CCGCCCCTGA TCTAGCTGCC 
AACAGAGCCA ACGACATTCA GGGTTACTTG GTCGACCTGC CATTGGAATT TCTGAACAAG 
GAGGTTCTCA CGCCGCCTGG AACTAGTAAG GAGGGCCTAA TCCCTACCTC TGTATGGACA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724472.3
CDS	174..3956
Translation	MADAGTVGGALPPYALTNSDEEHGSGEEDASEENSNNEEGEGVFRDCTDEAVVEHHNRCLPEFQFSLVDSEYDETLAFPDSVTILSNVGDKPVLVERKETDDDEEEFDDEENNSVVLRHEIPFTSIYGPSVKFNSFQRKVFIPGREIHVRIVDTERSVTTHLLNPNLYTIELTHGPFKWTIKRRYKHFNSLHQQLSFFRTSLNIPFPSRSHKEKRTTLKATAREMADESTLKDLPSHTKVKQTSTPLRAEGRSSKIAGSNANNAMAMISPNHSSILAGLTPRRIQKKRKKKKKRKLPRFPNRPESLVTVENLSVRIKQLEDYLYNLLNISLYRSHHETGKEGVILKRTGSTRPGQAGCNFFGCFQKNCCVRCNYFCSDVVCGTWRNRWFFVKETCFGYIRPTDGSIRAVILFDQGFDVSTGIYQTGMRKGLQVLTNNRHIVLKCWTRRKCKEWMQYLKNTANSYARDFTLPNPHMSFAPMRANTHATWYVDGAQYMSAVADGLEAALEEIYIADWWLSPEIYMKRPALDGDYWRLDKILLRKAEQGVRVFVLLYKEVEMALGINSYYSKSTLAKHENIKVMRHPDHARGGILLWAHHEKIVVIDQTYAFMGGIDLCYGRWDDHHHRLTDLGSISTSSFSGSTRRTPSLYFTKDDTDSAFGSRKSSRNAHYDTSAKERPPSPPPDEPNTSIELKTLKPGDRLLIPSTLVSSPGETPAESGIALEGMKLNTPEMERKNVLDRLKNNAMKGARMGKDFMHRLTATETEEKSAEVYTIESEEATDHEVNLNMASGGQEVAITTSSTQILSEFCGQAKYWFGKDYSNFILKDWMNLNSPFVDIIDRTTTPRMPWHDVGLCVVGTSARDVARHFIQRWNAMKLEKLRDNTRFPYLMPKSYHQVRLNPNIQQNRQQRVTCQLLRSVSAWSCGFIEADLVEQSIHDAYIQTITKAQHYVYIENQFFITMQLGMGVPGAYNNVRNQIGETLFKRIVRAHKERKPFRVYVIMPLLPGFEGDVGGSTGIAVRAITHWNYASISRGRTSILTRLQEAGIANPENYISFHSLRNHSFLNNTPITELIYVHSKLLIADDRVVICGSANINDRSMIGKRDSEIAAILMDEEFEDGRMNGKKYPSGVFAGRLRKYLFKEHLGLLESEGSSRSDLDINDPVCEKFWHGTWRRISMQNTEIYDEVFKCIPTDFVKTFASLRKYQEEPPLAKTAPDLAANRANDIQGYLVDLPLEFLNKEVLTPPGTSKEGLIPTSVWT

Target ORF information:

RefSeq Version	NM_165472.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phospholipase D, transcript variant G (Pld), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165472.3

ORF Insert Sequence:

ATGGCAGATG CCGGCACCGT TGGAGGCGCC TTGCCGCCCT ACGCACTTAC CAACTCGGAC 
GAAGAACATG GCAGCGGGGA AGAGGACGCG TCGGAGGAGA ACTCCAACAA TGAAGAGGGA 
GAGGGAGTGT TCCGGGACTG CACAGACGAA GCGGTTGTCG AGCATCATAA CCGCTGCCTG 
CCAGAATTTC AGTTCTCTCT AGTCGATTCT GAGTACGATG AGACCCTCGC TTTTCCTGAT 
TCTGTGACCA TTCTATCCAA CGTGGGCGAC AAGCCGGTGC TGGTGGAGCG CAAGGAGACG 
GACGATGATG AGGAGGAGTT CGACGACGAG GAAAACAACA GTGTAGTCCT GAGACACGAA 
ATACCATTTA CTAGCATATA CGGGCCGAGC GTCAAGTTCA ACTCGTTCCA GCGCAAGGTT 
TTCATCCCGG GCCGTGAGAT TCATGTTCGG ATCGTCGATA CGGAGCGTAG CGTCACTACA 
CATCTGCTAA ACCCCAATCT GTACACAATC GAGCTGACCC ACGGTCCCTT CAAGTGGACG 
ATCAAGCGGC GATACAAGCA CTTTAACTCG TTGCACCAGC AGCTCAGCTT TTTCCGCACC 
TCGCTCAACA TTCCTTTTCC CAGTCGCAGT CACAAGGAGA AGCGTACCAC TTTGAAAGCC 
ACAGCCAGAG AGATGGCTGA CGAGTCCACT CTAAAGGACC TTCCTTCTCA CACCAAGGTC 
AAACAAACTA GCACTCCGCT GAGGGCTGAA GGCAGAAGCA GTAAAATCGC GGGCAGTAAC 
GCCAACAATG CCATGGCTAT GATCAGTCCC AATCACAGCT CCATTCTGGC GGGTCTAACA 
CCACGACGCA TTCAAAAGAA GCGCAAAAAA AAGAAGAAAC GGAAGCTGCC GCGATTCCCA 
AACCGTCCTG AGAGTCTGGT CACCGTAGAG AATCTGAGCG TCAGAATAAA ACAGCTGGAG 
GACTACTTGT ACAACCTGCT GAACATCAGC TTGTACCGAT CTCACCATGA AACGGGCAAG 
GAAGGCGTGA TTTTAAAGCG AACTGGATCA ACGAGACCAG GGCAAGCAGG ATGCAATTTT 
TTTGGGTGCT TTCAAAAGAA CTGCTGTGTG CGCTGCAACT ACTTTTGCTC CGACGTAGTT 
TGCGGCACGT GGCGGAACCG ATGGTTTTTC GTAAAAGAGA CCTGCTTCGG CTACATCCGT 
CCAACAGACG GAAGCATCCG GGCAGTGATC CTCTTTGATC AGGGCTTCGA CGTTTCCACG 
GGTATCTATC AGACGGGCAT GCGCAAGGGC TTGCAGGTAC TGACGAACAA CCGTCACATT 
GTGCTCAAGT GCTGGACACG GCGTAAGTGT AAAGAGTGGA TGCAATACCT CAAGAACACG 
GCCAACTCGT ATGCGCGCGA CTTCACCCTG CCCAATCCGC ACATGTCCTT CGCTCCGATG 
CGCGCCAACA CTCATGCCAC GTGGTATGTG GACGGCGCCC AGTATATGTC AGCCGTGGCC 
GACGGCTTAG AGGCAGCCCT CGAGGAGATC TATATTGCCG ACTGGTGGCT CAGTCCCGAG 
ATATACATGA AGCGACCCGC ACTCGACGGA GACTACTGGC GATTGGACAA GATCCTGTTG 
CGCAAGGCCG AACAGGGAGT GCGCGTCTTT GTGCTGCTCT ACAAGGAGGT TGAAATGGCA 
CTTGGCATAA ACAGCTACTA CAGCAAGTCC ACGCTGGCCA AGCATGAAAA CATCAAGGTC 
ATGCGTCATC CGGACCATGC TAGAGGAGGT ATTCTGCTTT GGGCACATCA CGAAAAGATC 
GTCGTAATCG ACCAAACCTA TGCGTTTATG GGAGGTATTG ATTTGTGCTA TGGACGTTGG 
GATGATCACC ACCATCGGCT AACGGATCTG GGTAGCATAT CTACGTCATC TTTTTCTGGC 
AGCACGCGTC GAACGCCAAG TTTGTACTTC ACCAAAGACG ACACGGACTC AGCTTTCGGA 
TCACGTAAGT CCTCGCGAAA CGCTCACTAC GATACCTCCG CCAAGGAAAG GCCACCGTCC 
CCACCCCCGG ATGAGCCCAA TACTAGCATA GAGTTGAAAA CTCTTAAGCC TGGTGATCGA 
CTGCTTATAC CGTCTACGCT CGTTTCGAGT CCGGGTGAAA CTCCCGCAGA ATCGGGAATC 
GCTTTAGAAG GGATGAAACT CAACACCCCT GAAATGGAGC GTAAGAACGT ACTCGATCGC 
CTGAAGAACA ACGCGATGAA GGGCGCCCGT ATGGGCAAGG ACTTTATGCA CCGTCTAACA 
GCTACTGAGA CGGAGGAAAA ATCTGCGGAG GTGTACACTA TCGAGTCCGA GGAAGCTACG 
GACCACGAAG TCAACCTTAA CATGGCTTCA GGTGGGCAGG AAGTGGCGAT TACCACTAGC 
AGTACACAAA TACTCAGTGA GTTCTGCGGC CAGGCCAAGT ACTGGTTCGG CAAGGATTAC 
TCCAACTTTA TACTTAAAGA CTGGATGAAC CTAAACTCGC CGTTCGTGGA TATCATAGAT 
CGAACAACAA CACCGCGGAT GCCATGGCAC GACGTGGGTC TGTGTGTGGT GGGTACTTCC 
GCTAGGGATG TGGCCCGCCA CTTCATTCAG CGCTGGAATG CCATGAAGCT GGAGAAACTA 
CGCGATAACA CGAGATTCCC CTATTTGATG CCAAAAAGCT ATCACCAAGT GAGGCTCAAT 
CCGAACATTC AGCAAAACCG TCAGCAACGG GTCACGTGCC AGCTACTTCG AAGCGTCTCT 
GCCTGGAGCT GCGGCTTTAT AGAGGCGGAT CTTGTGGAGC AAAGCATCCA CGATGCCTAC 
ATCCAGACGA TCACCAAGGC GCAGCACTAC GTGTACATCG AAAACCAATT TTTTATCACT 
ATGCAGTTAG GCATGGGTGT GCCAGGTGCT TATAACAATG TGCGGAATCA AATCGGGGAA 
ACACTCTTTA AACGGATCGT TAGAGCGCAC AAGGAACGGA AGCCTTTCCG AGTTTATGTG 
ATTATGCCGC TCCTACCGGG CTTTGAGGGT GATGTCGGTG GCAGTACTGG GATAGCAGTC 
AGAGCAATTA CACACTGGAA CTATGCGTCC ATTTCCAGGG GACGCACATC AATTTTGACC 
CGCCTGCAGG AGGCGGGTAT TGCCAATCCG GAAAACTATA TCTCATTCCA CAGCCTGCGC 
AACCATTCTT TTTTGAATAA CACACCCATA ACAGAGTTGA TATATGTCCA CTCAAAGCTC 
TTGATAGCCG ACGATCGCGT TGTAATCTGC GGTTCGGCAA ACATTAACGA TCGCTCTATG 
ATCGGAAAGC GGGACTCCGA GATAGCGGCT ATTCTAATGG ACGAGGAGTT CGAGGACGGA 
CGCATGAATG GCAAGAAGTA TCCGAGCGGA GTGTTTGCCG GTCGCCTTCG AAAATACCTT 
TTTAAAGAAC ACTTAGGCCT CCTGGAAAGC GAAGGTTCCA GTCGGTCTGA CCTGGACATT 
AACGATCCTG TTTGTGAGAA GTTTTGGCAC GGCACCTGGC GTAGGATTTC AATGCAGAAC 
ACAGAGATTT ACGACGAGGT GTTTAAGTGC ATCCCCACTG ACTTTGTAAA AACCTTTGCC 
AGCCTTCGCA AATACCAGGA GGAGCCGCCT CTTGCCAAAA CCGCCCCTGA TCTAGCTGCC 
AACAGAGCCA ACGACATTCA GGGTTACTTG GTCGACCTGC CATTGGAATT TCTGAACAAG 
GAGGTTCTCA CGCCGCCTGG AACTAGTAAG GAGGGCCTAA TCCCTACCTC TGTATGGACA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pld ( NM_001144138.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137610.1 Drosophila melanogaster phospholipase D, transcript variant F (Pld), mRNA.

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CloneID	OFa09022
Clone ID Related Accession (Same CDS sequence)	NM_001144138.2
Accession Version	NM_001144138.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phospholipase D, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001144138.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGGCCA CGTGCTTGGA TACCAAAACC AGCAAGAACC ATGAGGCCCA GCAACTGCAA 
CTGCCCCGGC AGGCGTCATC GAACGCCAAC TTGCCACATT TGCAGGTCCG ACGTCATCGC 
CGCAGCATCT CACAGCTGAT GGCCAGCATG GTTGAACACC CCACTGACGA TGTTTACCGG 
CCGGCGCACT ATGGCGGCTA CGTTAACAGA GGCTACGATG ATTTAGACAG TTCCTACTAC 
TTTGCCCAGT ACGAGGCGAT GGCAGATGCC GGCACCGTTG GAGGCGCCTT GCCGCCCTAC 
GCACTTACCA ACTCGGACGA AGAACATGGC AGCGGGGAAG AGGACGCGTC GGAGGAGAAC 
TCCAACAATG AAGAGGGAGA GGGAGTGTTC CGGGACTGCA CAGACGAAGC GGTTGTCGAG 
CATCATAACC GCTGCCTGCC AGAATTTCAG TTCTCTCTAG TCGATTCTGA GTACGATGAG 
ACCCTCGCTT TTCCTGATTC TGTGACCATT CTATCCAACG TGGGCGACAA GCCGGTGCTG 
GTGGAGCGCA AGGAGACGGA CGATGATGAG GAGGAGTTCG ACGACGAGGA AAACAACAGT 
GTAGTCCTGA GACACGAAAT ACCATTTACT AGCATATACG GGCCGAGCGT CAAGTTCAAC 
TCGTTCCAGC GCAAGGTTTT CATCCCGGGC CGTGAGATTC ATGTTCGGAT CGTCGATACG 
GAGCGTAGCG TCACTACACA TCTGCTAAAC CCCAATCTGT ACACAATCGA GCTGACCCAC 
GGTCCCTTCA AGTGGACGAT CAAGCGGCGA TACAAGCACT TTAACTCGTT GCACCAGCAG 
CTCAGCTTTT TCCGCACCTC GCTCAACATT CCTTTTCCCA GTCGCAGTCA CAAGGAGAAG 
CGTACCACTT TGAAAGCCAC AGCCAGAGAG ATGGCTGACG AGTCCACTCT AAAGGACCTT 
CCTTCTCACA CCAAGGTCAA ACAAACTAGC ACTCCGCTGA GGGCTGAAGG CAGAAGCAGT 
AAAATCGCGG GCAGTAACGC CAACAATGCC ATGGCTATGA TCAGTCCCAA TCACAGCTCC 
ATTCTGGCGG GTCTAACACC ACGACGCATT CAAAAGAAGC GCAAAAAAAA GAAGAAACGG 
AAGCTGCCGC GATTCCCAAA CCGTCCTGAG AGTCTGGTCA CCGTAGAGAA TCTGAGCGTC 
AGAATAAAAC AGCTGGAGGA CTACTTGTAC AACCTGCTGA ACATCAGCTT GTACCGATCT 
CACCATGAAA CGCTAAACTT CGTTGAAGTG TCTAATGTGT CCTTTGTTCC GGGAATGGGA 
ATTAAGGGCA AGGAAGGCGT GATTTTAAAG CGAACTGGAT CAACGAGACC AGGGCAAGCA 
GGATGCAATT TTTTTGGGTG CTTTCAAAAG AACTGCTGTG TGCGCTGCAA CTACTTTTGC 
TCCGACGTAG TTTGCGGCAC GTGGCGGAAC CGATGGTTTT TCGTAAAAGA GACCTGCTTC 
GGCTACATCC GTCCAACAGA CGGAAGCATC CGGGCAGTGA TCCTCTTTGA TCAGGGCTTC 
GACGTTTCCA CGGGTATCTA TCAGACGGGC ATGCGCAAGG GCTTGCAGGT ACTGACGAAC 
AACCGTCACA TTGTGCTCAA GTGCTGGACA CGGCGTAAGT GTAAAGAGTG GATGCAATAC 
CTCAAGAACA CGGCCAACTC GTATGCGCGC GACTTCACCC TGCCCAATCC GCACATGTCC 
TTCGCTCCGA TGCGCGCCAA CACTCATGCC ACGTGGTATG TGGACGGCGC CCAGTATATG 
TCAGCCGTGG CCGACGGCTT AGAGGCAGCC CTCGAGGAGA TCTATATTGC CGACTGGTGG 
CTCAGTCCCG AGATATACAT GAAGCGACCC GCACTCGACG GAGACTACTG GCGATTGGAC 
AAGATCCTGT TGCGCAAGGC CGAACAGGGA GTGCGCGTCT TTGTGCTGCT CTACAAGGAG 
GTTGAAATGG CACTTGGCAT AAACAGCTAC TACAGCAAGT CCACGCTGGC CAAGCATGAA 
AACATCAAGG TCATGCGTCA TCCGGACCAT GCTAGAGGAG GTATTCTGCT TTGGGCACAT 
CACGAAAAGA TCGTCGTAAT CGACCAAACC TATGCGTTTA TGGGAGGTAT TGATTTGTGC 
TATGGACGTT GGGATGATCA CCACCATCGG CTAACGGATC TGGGTAGCAT ATCTACGTCA 
TCTTTTTCTG GCAGCACGCG TCGAACGCCA AGTTTGTACT TCACCAAAGA CGACACGGAC 
TCAGCTTTCG GATCACGTAA GTCCTCGCGA AACGCTCACT ACGATACCTC CGCCAAGGAA 
AGGCCACCGT CCCCACCCCC GGATGAGCCC AATACTAGCA TAGAGTTGAA AACTCTTAAG 
CCTGGTGATC GACTGCTTAT ACCGTCTACG CTCGTTTCGA GTCCGGGTGA AACTCCCGCA 
GAATCGGGAA TCGCTTTAGA AGGGATGAAA CTCAACACCC CTGAAATGGA GCGTAAGAAC 
GTACTCGATC GCCTGAAGAA CAACGCGATG AAGGGCGCCC GTATGGGCAA GGACTTTATG 
CACCGTCTAA CAGCTACTGA GACGGAGGAA AAATCTGCGG AGGTGTACAC TATCGAGTCC 
GAGGAAGCTA CGGACCACGA AGTCAACCTT AACATGGCTT CAGGTGGGCA GGAAGTGGCG 
ATTACCACTA GCAGTACACA AATACTCAGT GAGTTCTGCG GCCAGGCCAA GTACTGGTTC 
GGCAAGGATT ACTCCAACTT TATACTTAAA GACTGGATGA ACCTAAACTC GCCGTTCGTG 
GATATCATAG ATCGAACAAC AACACCGCGG ATGCCATGGC ACGACGTGGG TCTGTGTGTG 
GTGGGTACTT CCGCTAGGGA TGTGGCCCGC CACTTCATTC AGCGCTGGAA TGCCATGAAG 
CTGGAGAAAC TACGCGATAA CACGAGATTC CCCTATTTGA TGCCAAAAAG CTATCACCAA 
GTGAGGCTCA ATCCGAACAT TCAGCAAAAC CGTCAGCAAC GGGTCACGTG CCAGCTACTT 
CGAAGCGTCT CTGCCTGGAG CTGCGGCTTT ATAGAGGCGG ATCTTGTGGA GCAAAGCATC 
CACGATGCCT ACATCCAGAC GATCACCAAG GCGCAGCACT ACGTGTACAT CGAAAACCAA 
TTTTTTATCA CTATGCAGTT AGGCATGGGT GTGCCAGGTG CTTATAACAA TGTGCGGAAT 
CAAATCGGGG AAACACTCTT TAAACGGATC GTTAGAGCGC ACAAGGAACG GAAGCCTTTC 
CGAGTTTATG TGATTATGCC GCTCCTACCG GGCTTTGAGG GTGATGTCGG TGGCAGTACT 
GGGATAGCAG TCAGAGCAAT TACACACTGG AACTATGCGT CCATTTCCAG GGGACGCACA 
TCAATTTTGA CCCGCCTGCA GGAGGCGGGT ATTGCCAATC CGGAAAACTA TATCTCATTC 
CACAGCCTGC GCAACCATTC TTTTTTGAAT AACACACCCA TAACAGAGTT GATATATGTC 
CACTCAAAGC TCTTGATAGC CGACGATCGC GTTGTAATCT GCGGTTCGGC AAACATTAAC 
GATCGCTCTA TGATCGGAAA GCGGGACTCC GAGATAGCGG CTATTCTAAT GGACGAGGAG 
TTCGAGGACG GACGCATGAA TGGCAAGAAG TATCCGAGCG GAGTGTTTGC CGGTCGCCTT 
CGAAAATACC TTTTTAAAGA ACACTTAGGC CTCCTGGAAA GCGAAGGTTC CAGTCGGTCT 
GACCTGGACA TTAACGATCC TGTTTGTGAG AAGTTTTGGC ACGGCACCTG GCGTAGGATT 
TCAATGCAGA ACACAGAGAT TTACGACGAG GTGTTTAAGT GCATCCCCAC TGACTTTGTA 
AAAACCTTTG CCAGCCTTCG CAAATACCAG GAGGAGCCGC CTCTTGCCAA AACCGCCCCT 
GATCTAGCTG CCAACAGAGC CAACGACATT CAGGGTTACT TGGTCGACCT GCCATTGGAA 
TTTCTGAACA AGGAGGTTCT CACGCCGCCT GGAACTAGTA AGGAGGGCCT AATCCCTACC 
TCTGTATGGA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137610.1
CDS	237..4331
Translation	MMATCLDTKTSKNHEAQQLQLPRQASSNANLPHLQVRRHRRSISQLMASMVEHPTDDVYRPAHYGGYVNRGYDDLDSSYYFAQYEAMADAGTVGGALPPYALTNSDEEHGSGEEDASEENSNNEEGEGVFRDCTDEAVVEHHNRCLPEFQFSLVDSEYDETLAFPDSVTILSNVGDKPVLVERKETDDDEEEFDDEENNSVVLRHEIPFTSIYGPSVKFNSFQRKVFIPGREIHVRIVDTERSVTTHLLNPNLYTIELTHGPFKWTIKRRYKHFNSLHQQLSFFRTSLNIPFPSRSHKEKRTTLKATAREMADESTLKDLPSHTKVKQTSTPLRAEGRSSKIAGSNANNAMAMISPNHSSILAGLTPRRIQKKRKKKKKRKLPRFPNRPESLVTVENLSVRIKQLEDYLYNLLNISLYRSHHETLNFVEVSNVSFVPGMGIKGKEGVILKRTGSTRPGQAGCNFFGCFQKNCCVRCNYFCSDVVCGTWRNRWFFVKETCFGYIRPTDGSIRAVILFDQGFDVSTGIYQTGMRKGLQVLTNNRHIVLKCWTRRKCKEWMQYLKNTANSYARDFTLPNPHMSFAPMRANTHATWYVDGAQYMSAVADGLEAALEEIYIADWWLSPEIYMKRPALDGDYWRLDKILLRKAEQGVRVFVLLYKEVEMALGINSYYSKSTLAKHENIKVMRHPDHARGGILLWAHHEKIVVIDQTYAFMGGIDLCYGRWDDHHHRLTDLGSISTSSFSGSTRRTPSLYFTKDDTDSAFGSRKSSRNAHYDTSAKERPPSPPPDEPNTSIELKTLKPGDRLLIPSTLVSSPGETPAESGIALEGMKLNTPEMERKNVLDRLKNNAMKGARMGKDFMHRLTATETEEKSAEVYTIESEEATDHEVNLNMASGGQEVAITTSSTQILSEFCGQAKYWFGKDYSNFILKDWMNLNSPFVDIIDRTTTPRMPWHDVGLCVVGTSARDVARHFIQRWNAMKLEKLRDNTRFPYLMPKSYHQVRLNPNIQQNRQQRVTCQLLRSVSAWSCGFIEADLVEQSIHDAYIQTITKAQHYVYIENQFFITMQLGMGVPGAYNNVRNQIGETLFKRIVRAHKERKPFRVYVIMPLLPGFEGDVGGSTGIAVRAITHWNYASISRGRTSILTRLQEAGIANPENYISFHSLRNHSFLNNTPITELIYVHSKLLIADDRVVICGSANINDRSMIGKRDSEIAAILMDEEFEDGRMNGKKYPSGVFAGRLRKYLFKEHLGLLESEGSSRSDLDINDPVCEKFWHGTWRRISMQNTEIYDEVFKCIPTDFVKTFASLRKYQEEPPLAKTAPDLAANRANDIQGYLVDLPLEFLNKEVLTPPGTSKEGLIPTSVWT

Target ORF information:

RefSeq Version	NM_001144138.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phospholipase D, transcript variant F (Pld), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144138.2

ORF Insert Sequence:

ATGATGGCCA CGTGCTTGGA TACCAAAACC AGCAAGAACC ATGAGGCCCA GCAACTGCAA 
CTGCCCCGGC AGGCGTCATC GAACGCCAAC TTGCCACATT TGCAGGTCCG ACGTCATCGC 
CGCAGCATCT CACAGCTGAT GGCCAGCATG GTTGAACACC CCACTGACGA TGTTTACCGG 
CCGGCGCACT ATGGCGGCTA CGTTAACAGA GGCTACGATG ATTTAGACAG TTCCTACTAC 
TTTGCCCAGT ACGAGGCGAT GGCAGATGCC GGCACCGTTG GAGGCGCCTT GCCGCCCTAC 
GCACTTACCA ACTCGGACGA AGAACATGGC AGCGGGGAAG AGGACGCGTC GGAGGAGAAC 
TCCAACAATG AAGAGGGAGA GGGAGTGTTC CGGGACTGCA CAGACGAAGC GGTTGTCGAG 
CATCATAACC GCTGCCTGCC AGAATTTCAG TTCTCTCTAG TCGATTCTGA GTACGATGAG 
ACCCTCGCTT TTCCTGATTC TGTGACCATT CTATCCAACG TGGGCGACAA GCCGGTGCTG 
GTGGAGCGCA AGGAGACGGA CGATGATGAG GAGGAGTTCG ACGACGAGGA AAACAACAGT 
GTAGTCCTGA GACACGAAAT ACCATTTACT AGCATATACG GGCCGAGCGT CAAGTTCAAC 
TCGTTCCAGC GCAAGGTTTT CATCCCGGGC CGTGAGATTC ATGTTCGGAT CGTCGATACG 
GAGCGTAGCG TCACTACACA TCTGCTAAAC CCCAATCTGT ACACAATCGA GCTGACCCAC 
GGTCCCTTCA AGTGGACGAT CAAGCGGCGA TACAAGCACT TTAACTCGTT GCACCAGCAG 
CTCAGCTTTT TCCGCACCTC GCTCAACATT CCTTTTCCCA GTCGCAGTCA CAAGGAGAAG 
CGTACCACTT TGAAAGCCAC AGCCAGAGAG ATGGCTGACG AGTCCACTCT AAAGGACCTT 
CCTTCTCACA CCAAGGTCAA ACAAACTAGC ACTCCGCTGA GGGCTGAAGG CAGAAGCAGT 
AAAATCGCGG GCAGTAACGC CAACAATGCC ATGGCTATGA TCAGTCCCAA TCACAGCTCC 
ATTCTGGCGG GTCTAACACC ACGACGCATT CAAAAGAAGC GCAAAAAAAA GAAGAAACGG 
AAGCTGCCGC GATTCCCAAA CCGTCCTGAG AGTCTGGTCA CCGTAGAGAA TCTGAGCGTC 
AGAATAAAAC AGCTGGAGGA CTACTTGTAC AACCTGCTGA ACATCAGCTT GTACCGATCT 
CACCATGAAA CGCTAAACTT CGTTGAAGTG TCTAATGTGT CCTTTGTTCC GGGAATGGGA 
ATTAAGGGCA AGGAAGGCGT GATTTTAAAG CGAACTGGAT CAACGAGACC AGGGCAAGCA 
GGATGCAATT TTTTTGGGTG CTTTCAAAAG AACTGCTGTG TGCGCTGCAA CTACTTTTGC 
TCCGACGTAG TTTGCGGCAC GTGGCGGAAC CGATGGTTTT TCGTAAAAGA GACCTGCTTC 
GGCTACATCC GTCCAACAGA CGGAAGCATC CGGGCAGTGA TCCTCTTTGA TCAGGGCTTC 
GACGTTTCCA CGGGTATCTA TCAGACGGGC ATGCGCAAGG GCTTGCAGGT ACTGACGAAC 
AACCGTCACA TTGTGCTCAA GTGCTGGACA CGGCGTAAGT GTAAAGAGTG GATGCAATAC 
CTCAAGAACA CGGCCAACTC GTATGCGCGC GACTTCACCC TGCCCAATCC GCACATGTCC 
TTCGCTCCGA TGCGCGCCAA CACTCATGCC ACGTGGTATG TGGACGGCGC CCAGTATATG 
TCAGCCGTGG CCGACGGCTT AGAGGCAGCC CTCGAGGAGA TCTATATTGC CGACTGGTGG 
CTCAGTCCCG AGATATACAT GAAGCGACCC GCACTCGACG GAGACTACTG GCGATTGGAC 
AAGATCCTGT TGCGCAAGGC CGAACAGGGA GTGCGCGTCT TTGTGCTGCT CTACAAGGAG 
GTTGAAATGG CACTTGGCAT AAACAGCTAC TACAGCAAGT CCACGCTGGC CAAGCATGAA 
AACATCAAGG TCATGCGTCA TCCGGACCAT GCTAGAGGAG GTATTCTGCT TTGGGCACAT 
CACGAAAAGA TCGTCGTAAT CGACCAAACC TATGCGTTTA TGGGAGGTAT TGATTTGTGC 
TATGGACGTT GGGATGATCA CCACCATCGG CTAACGGATC TGGGTAGCAT ATCTACGTCA 
TCTTTTTCTG GCAGCACGCG TCGAACGCCA AGTTTGTACT TCACCAAAGA CGACACGGAC 
TCAGCTTTCG GATCACGTAA GTCCTCGCGA AACGCTCACT ACGATACCTC CGCCAAGGAA 
AGGCCACCGT CCCCACCCCC GGATGAGCCC AATACTAGCA TAGAGTTGAA AACTCTTAAG 
CCTGGTGATC GACTGCTTAT ACCGTCTACG CTCGTTTCGA GTCCGGGTGA AACTCCCGCA 
GAATCGGGAA TCGCTTTAGA AGGGATGAAA CTCAACACCC CTGAAATGGA GCGTAAGAAC 
GTACTCGATC GCCTGAAGAA CAACGCGATG AAGGGCGCCC GTATGGGCAA GGACTTTATG 
CACCGTCTAA CAGCTACTGA GACGGAGGAA AAATCTGCGG AGGTGTACAC TATCGAGTCC 
GAGGAAGCTA CGGACCACGA AGTCAACCTT AACATGGCTT CAGGTGGGCA GGAAGTGGCG 
ATTACCACTA GCAGTACACA AATACTCAGT GAGTTCTGCG GCCAGGCCAA GTACTGGTTC 
GGCAAGGATT ACTCCAACTT TATACTTAAA GACTGGATGA ACCTAAACTC GCCGTTCGTG 
GATATCATAG ATCGAACAAC AACACCGCGG ATGCCATGGC ACGACGTGGG TCTGTGTGTG 
GTGGGTACTT CCGCTAGGGA TGTGGCCCGC CACTTCATTC AGCGCTGGAA TGCCATGAAG 
CTGGAGAAAC TACGCGATAA CACGAGATTC CCCTATTTGA TGCCAAAAAG CTATCACCAA 
GTGAGGCTCA ATCCGAACAT TCAGCAAAAC CGTCAGCAAC GGGTCACGTG CCAGCTACTT 
CGAAGCGTCT CTGCCTGGAG CTGCGGCTTT ATAGAGGCGG ATCTTGTGGA GCAAAGCATC 
CACGATGCCT ACATCCAGAC GATCACCAAG GCGCAGCACT ACGTGTACAT CGAAAACCAA 
TTTTTTATCA CTATGCAGTT AGGCATGGGT GTGCCAGGTG CTTATAACAA TGTGCGGAAT 
CAAATCGGGG AAACACTCTT TAAACGGATC GTTAGAGCGC ACAAGGAACG GAAGCCTTTC 
CGAGTTTATG TGATTATGCC GCTCCTACCG GGCTTTGAGG GTGATGTCGG TGGCAGTACT 
GGGATAGCAG TCAGAGCAAT TACACACTGG AACTATGCGT CCATTTCCAG GGGACGCACA 
TCAATTTTGA CCCGCCTGCA GGAGGCGGGT ATTGCCAATC CGGAAAACTA TATCTCATTC 
CACAGCCTGC GCAACCATTC TTTTTTGAAT AACACACCCA TAACAGAGTT GATATATGTC 
CACTCAAAGC TCTTGATAGC CGACGATCGC GTTGTAATCT GCGGTTCGGC AAACATTAAC 
GATCGCTCTA TGATCGGAAA GCGGGACTCC GAGATAGCGG CTATTCTAAT GGACGAGGAG 
TTCGAGGACG GACGCATGAA TGGCAAGAAG TATCCGAGCG GAGTGTTTGC CGGTCGCCTT 
CGAAAATACC TTTTTAAAGA ACACTTAGGC CTCCTGGAAA GCGAAGGTTC CAGTCGGTCT 
GACCTGGACA TTAACGATCC TGTTTGTGAG AAGTTTTGGC ACGGCACCTG GCGTAGGATT 
TCAATGCAGA ACACAGAGAT TTACGACGAG GTGTTTAAGT GCATCCCCAC TGACTTTGTA 
AAAACCTTTG CCAGCCTTCG CAAATACCAG GAGGAGCCGC CTCTTGCCAA AACCGCCCCT 
GATCTAGCTG CCAACAGAGC CAACGACATT CAGGGTTACT TGGTCGACCT GCCATTGGAA 
TTTCTGAACA AGGAGGTTCT CACGCCGCCT GGAACTAGTA AGGAGGGCCT AATCCCTACC 
TCTGTATGGA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pld cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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