Itch cDNA ORF clone, Dipodomys ordii(Ord's kangaroo rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Itch
Entrez Gene ID	105981497
Full Name	itchy E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Dipodomys ordii(Ord's kangaroo rat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013010689.1	XP_012866143.1	E3 ubiquitin-protein ligase Itchy homolog

Pathways from BioSystems
Gene Ontology

The following Itch gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Itch cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODi136486	XM_013010689.1 Latest version!	Dipodomys ordii itchy E3 ubiquitin protein ligase (Itch), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Itch ( XM_013010689.1 ) cDNA ORF clone, Dipodomys ordii(Ord's kangaroo rat) -> XP_012866143.1 Dipodomys ordii itchy E3 ubiquitin protein ligase (Itch), mRNA.

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CloneID	ODi136486
Clone ID Related Accession (Same CDS sequence)	XM_013010689.1
Accession Version	XM_013010689.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-25
Organism	Dipodomys ordii(Ord's kangaroo rat)
Product	E3 ubiquitin-protein ligase Itchy homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012267229.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dipodomys ordii Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTCTGACA GTGGATCACA GCTTGGTTCA ATGGGTAGCC TGACCATGAA ATCACAACTT 
CAGATCACTG TCATCTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGACCAAGT 
CCTTATGTAG AAGTCATAGT AGATGGACAG TCAAAGAAGA CAGAAAAGTG TAACAATACA 
AACAGTCCCA AATGGAAGCA ACCCCTTACA GTTATTGTTA CCCCTGTGAG TAAATTACGT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCACTA 
GATGTTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTGGT TGTGACTTTG 
CAGCTTGTAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTGCAGT TAGATGCTGA TGTTGTTACT AATGGTGAAA CTACATGTTC AGAAAGTAAA 
GTGAACACAA ATGGATCAGA TGACCCTGAT GATTCAGGGT CTGGTGAAAA TAGGAGAACC 
AATGGGAACA ATTCTCCATC ACTCTCCAAT GGTGGGTTTA AGCCTTCTAG ACCTCCAAGA 
CCTTCACGAC CACCTCCACC CACACCTCGA AGACCAGCAT CTGTCAATGG CTCACCATCT 
ACCACTTCTG AAAGTGATGG ATCTAGTACT GGTTCTCTAC CATCAACAAA TACAAATACC 
TCTGAAGGAG CAACATCTGG ATTAATAATT CCTCTAACTA TTTCCGGAGG CTCAGGTCCT 
AGACCATTAA ATACTGTAAC TCAAGCTCCC CTACCACCTG GGTGGGAACA GAGAGTGGAC 
CAGCATGGGC GAGTTTATTA TGTAGATCAT GTTGAAAAAA GAACAACATG GGATAGACCT 
GAACCTCTAC CTCCTGGCTG GGAGCGGCGG GTTGACAATA TGGGACGTAT TTATTATGTT 
GACCATTTTA CAAGAACAAC AACATGGCAG AGGCCAACAC TGGAATCTGT CCGCAACTAT 
GAACAATGGC AGCTACAACG TAGCCAACTT CAAGGAGCAA TGCAGCAGTT TAATCAGAGA 
TTTATTTATG GGAATCAAGA TTTGTTTGCT ACATCACAAA ATAAAGAATT TGATCCGCTT 
GGTCCTTTGC CACCTGGGTG GGAGAAAAGA ACTGATAACA ATGGCAGAGT ATATTTTGTC 
AACCACAACA CTCGAATTAC TCAATGGGAA GACCCCCGAA GTCAAGGTCA ATTAAATGAA 
AAGCCCCTAC CTGAAGGCTG GGAAATGAGA TTCACAGTGG ATGGGATTCC ATATTTTGTG 
GACCACAATA GAAGAACAAC AACCTACATA GATCCCCGAA CAGGAAAATC TGCCTTAGAC 
AATGGACCCC AAATAGCCTA TGTTCGAGAC TTCAAAGCAA AGGTTCAGTA CTTCCGGTTT 
TGGTGTCAGC AACTGGCCAT GCCACAGCAC ATAAAGATTA CAGTGACAAG AAAAACATTG 
TTTGAAGATT CCTTTCAGCA GATAATGAGC TTCAGTCCCC AAGATCTGCG AAGACGTTTG 
TGGGTGATTT TTCCAGGAGA AGAAGGTTTA GATTATGGAG GTGTAGCAAG AGAATGGTTC 
TTTCTTTTGT CACATGAAGT GTTGAACCCC ATGTATTGCC TGTTTGAATA TGCAGGGAAG 
GATAATTACT GCTTACAGAT AAACCCTGCA TCTTACATCA ATCCTGATCA TCTGAAATAT 
TTTCGCTTTA TTGGCAGGTT TATTGCCATG GCTCTATTCC ATGGAAAATT CATAGACACG 
GGTTTTTCCT TACCATTCTA TAAACGTATC TTGAACAAAC CAGTTGGACT TAAAGATTTA 
GAATCTATTG ATCCAGAATT TTACAATTCT CTCATCTGGG TTAAAGAGAA CAATATTGAA 
GAATGTGGCT TGGAAATGTA CTTCTCTGTG GATAAAGAAA TTTTAGGTGA AATTAAGAGC 
CATGATTTGA AACCCAATGG TGGCAATATT CTTGTGACAG AAGAAAATAA AGAGGAATAT 
ATAAGAATGG TGGCTGAATG GAGGTTGTCT CGAGGAGTTG AAGAACAGAC ACAAGCTTTC 
TTTGAGGGCT TTAATGAAAT TCTTCCCCAG CAGTATTTGC AGTACTTCGA TGCAAAGGAA 
TTAGAGGTAC TTTTATGTGG AATGCAAGAG ATTGATTTGA ATGACTGGCA AAGACATGCT 
ATCTACCGCC ATTACACTAG GACAAGCAAA CAAATCATGT GGTTTTGGCA GTTTGTTAAA 
GAAATTGATA ATGAGAAGAG AATGAGACTT CTGCAATTTG TTACTGGAAC TTGCCGATTG 
CCAGTAGGAG GATTTGCTGA CCTCATGGGG AGCAATGGGC CACAGAAATT CTGCATTGAA 
AAAGTTGGGA AAGAAAATTG GCTACCCAGA AGTCATACCT GTTTTAATCG CCTGGACCTT 
CCACCCTATA AGAGCTATGA GCAACTGAAG GAGAAGCTGT TGTTTGCCAT TGAAGAAACA 
GAAGGATTTG GACAAGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012866143.1
CDS	189..2729
Translation	MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVIVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLRFRVWSHQTLKSDVLLGTAALDVYETLKSNNMKLEEVVVTLQLVGDKEPTETIGDLSICLDGLQLDADVVTNGETTCSESKVNTNGSDDPDDSGSGENRRTNGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSTTSESDGSSTGSLPSTNTNTSEGATSGLIIPLTISGGSGPRPLNTVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQNKEFDPLGPLPPGWEKRTDNNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_013010689.1
Organism	Dipodomys ordii(Ord's kangaroo rat)
Definition	Dipodomys ordii itchy E3 ubiquitin protein ligase (Itch), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013010689.1

ORF Insert Sequence:

ATGTCTGACA GTGGATCACA GCTTGGTTCA ATGGGTAGCC TGACCATGAA ATCACAACTT 
CAGATCACTG TCATCTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGACCAAGT 
CCTTATGTAG AAGTCATAGT AGATGGACAG TCAAAGAAGA CAGAAAAGTG TAACAATACA 
AACAGTCCCA AATGGAAGCA ACCCCTTACA GTTATTGTTA CCCCTGTGAG TAAATTACGT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCACTA 
GATGTTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTGGT TGTGACTTTG 
CAGCTTGTAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTGCAGT TAGATGCTGA TGTTGTTACT AATGGTGAAA CTACATGTTC AGAAAGTAAA 
GTGAACACAA ATGGATCAGA TGACCCTGAT GATTCAGGGT CTGGTGAAAA TAGGAGAACC 
AATGGGAACA ATTCTCCATC ACTCTCCAAT GGTGGGTTTA AGCCTTCTAG ACCTCCAAGA 
CCTTCACGAC CACCTCCACC CACACCTCGA AGACCAGCAT CTGTCAATGG CTCACCATCT 
ACCACTTCTG AAAGTGATGG ATCTAGTACT GGTTCTCTAC CATCAACAAA TACAAATACC 
TCTGAAGGAG CAACATCTGG ATTAATAATT CCTCTAACTA TTTCCGGAGG CTCAGGTCCT 
AGACCATTAA ATACTGTAAC TCAAGCTCCC CTACCACCTG GGTGGGAACA GAGAGTGGAC 
CAGCATGGGC GAGTTTATTA TGTAGATCAT GTTGAAAAAA GAACAACATG GGATAGACCT 
GAACCTCTAC CTCCTGGCTG GGAGCGGCGG GTTGACAATA TGGGACGTAT TTATTATGTT 
GACCATTTTA CAAGAACAAC AACATGGCAG AGGCCAACAC TGGAATCTGT CCGCAACTAT 
GAACAATGGC AGCTACAACG TAGCCAACTT CAAGGAGCAA TGCAGCAGTT TAATCAGAGA 
TTTATTTATG GGAATCAAGA TTTGTTTGCT ACATCACAAA ATAAAGAATT TGATCCGCTT 
GGTCCTTTGC CACCTGGGTG GGAGAAAAGA ACTGATAACA ATGGCAGAGT ATATTTTGTC 
AACCACAACA CTCGAATTAC TCAATGGGAA GACCCCCGAA GTCAAGGTCA ATTAAATGAA 
AAGCCCCTAC CTGAAGGCTG GGAAATGAGA TTCACAGTGG ATGGGATTCC ATATTTTGTG 
GACCACAATA GAAGAACAAC AACCTACATA GATCCCCGAA CAGGAAAATC TGCCTTAGAC 
AATGGACCCC AAATAGCCTA TGTTCGAGAC TTCAAAGCAA AGGTTCAGTA CTTCCGGTTT 
TGGTGTCAGC AACTGGCCAT GCCACAGCAC ATAAAGATTA CAGTGACAAG AAAAACATTG 
TTTGAAGATT CCTTTCAGCA GATAATGAGC TTCAGTCCCC AAGATCTGCG AAGACGTTTG 
TGGGTGATTT TTCCAGGAGA AGAAGGTTTA GATTATGGAG GTGTAGCAAG AGAATGGTTC 
TTTCTTTTGT CACATGAAGT GTTGAACCCC ATGTATTGCC TGTTTGAATA TGCAGGGAAG 
GATAATTACT GCTTACAGAT AAACCCTGCA TCTTACATCA ATCCTGATCA TCTGAAATAT 
TTTCGCTTTA TTGGCAGGTT TATTGCCATG GCTCTATTCC ATGGAAAATT CATAGACACG 
GGTTTTTCCT TACCATTCTA TAAACGTATC TTGAACAAAC CAGTTGGACT TAAAGATTTA 
GAATCTATTG ATCCAGAATT TTACAATTCT CTCATCTGGG TTAAAGAGAA CAATATTGAA 
GAATGTGGCT TGGAAATGTA CTTCTCTGTG GATAAAGAAA TTTTAGGTGA AATTAAGAGC 
CATGATTTGA AACCCAATGG TGGCAATATT CTTGTGACAG AAGAAAATAA AGAGGAATAT 
ATAAGAATGG TGGCTGAATG GAGGTTGTCT CGAGGAGTTG AAGAACAGAC ACAAGCTTTC 
TTTGAGGGCT TTAATGAAAT TCTTCCCCAG CAGTATTTGC AGTACTTCGA TGCAAAGGAA 
TTAGAGGTAC TTTTATGTGG AATGCAAGAG ATTGATTTGA ATGACTGGCA AAGACATGCT 
ATCTACCGCC ATTACACTAG GACAAGCAAA CAAATCATGT GGTTTTGGCA GTTTGTTAAA 
GAAATTGATA ATGAGAAGAG AATGAGACTT CTGCAATTTG TTACTGGAAC TTGCCGATTG 
CCAGTAGGAG GATTTGCTGA CCTCATGGGG AGCAATGGGC CACAGAAATT CTGCATTGAA 
AAAGTTGGGA AAGAAAATTG GCTACCCAGA AGTCATACCT GTTTTAATCG CCTGGACCTT 
CCACCCTATA AGAGCTATGA GCAACTGAAG GAGAAGCTGT TGTTTGCCAT TGAAGAAACA 
GAAGGATTTG GACAAGAGTA A